CN105944149A - Absorbable cartilage repair system and preparation method thereof - Google Patents
Absorbable cartilage repair system and preparation method thereof Download PDFInfo
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- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/06—Materials or treatment for tissue regeneration for cartilage reconstruction, e.g. meniscus
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Abstract
The invention belongs to the technical field of cartilage repair, in particular to an absorbable cartilage repair system. The system is composed of an absorbable cartilage repair stent and autoblood, is in a gel state at the human physiological temperature of 37 DEG C, and is implanted into a cartilage defect part to repair cartilage tissue. The absorbable cartilage repair stent is mainly prepared from chitosan, glycerophosphate, a pH value regulator, an osmotic pressure regulator and water. The repair system has the advantages that the repair system has sterility, apyrogeneity and non-immunogenicity, is good in biocompatibility and is degradable and absorbable; chitosan and autoblood can form stable stent gel after being mixed, the stent gel is stable and reliable after being implanted into a bone defect area, and growth factors in autoblood can promote the growth of cartilage cells and shorten the formation time of cartilage tissue; cartilage repair can be completed through a minimally invasive operation, time is saved for doctors, and the pain of a patient is relieved; the risks of immunogenicity and animal virus infection and contagion are avoided.
Description
Technical field
The invention belongs to cartilage repair techniques field, being specifically related to a kind of absorbable cartilage repair systems, this system is by caning absorb repair of cartilage support and autologous blood forms, gel at Human Physiology temperature 37 DEG C, repair in cartilage defect, the reparation of cartilaginous tissue can be realized.
Background technology
Osteoarthritis is moved back sexually transmitted disease (STD) for a kind of row and is become, and is articular cartilage degeneration damage, joint margins and the subchondral bone reactive hyperplasia caused due to many factors.Clinical manifestation is the slowly arthralgia of development, tenderness, stiff, arthroncus, limitation of activity and joint deformity etc..X-ray plain film later stage visible joint narrow gaps, CT and MRI checks and can find articular cartilage and the abnormal change of subchondral bone matter in getting up early.At present, in more than 60 years old old man of China, having the people of 55% to suffer from osteoarthritis, the whole nation there are about 1.2 hundred million people and suffers from this disease.
Currently the treatment to osteoarthritis includes following aspect: for early-stage cases, physical therapy, motion exercise treatment can be carried out, make analgesic medications, intraarticular injection hyaluronic acid series lubricant agent, but, these Therapeutic Method all can only the symptom of relief from osteoarthritis to a certain extent, it is impossible to enough reverses osteoarthritic condition progress, thoroughly cure this disease；For late case, under conditions of systemic conditions is resistant to operation, carry out artificial joint replacement, be generally acknowledge at present elimination pain, correction deformity, improve the effective ways of function, but, it has strict demand to patient age, health, and post-operative complication is more, such as infection, venae profunda conducted etc., and every example artificial joint only fee of material is accomplished by 4～60,000 yuan.
The focus of osteoarthritis treatment research it is currently for the research in terms of Regeneration of Articular Cartilage and reparation.Current trial has following a few class:
The most micro-fracture method: for arthroscopic surgery, remove calcified cartilage, turn hole in defect, makes bone marrow and blood ooze out, and forms sludged blood, and Induction of chondrocytes grows, and shortcoming: non-real cartilage, convalescent period length needs half a year to more than 1 year.
2. bone and cartilage autotransplantation: by self non-bearing cartilage transplantation to defective region, shortcoming: operation technique is loaded down with trivial details, human body wound is big, and cartilage is limited for district.
3. Autologous Chondrocyte transplants (ACI): takes from body articular chondrocytes and cultivates in vitro, replants into defective region, shortcoming: need second operation, condition of culture harsh, need the laboratory of corresponding certification to carry out.
4. platelet rich plasma gel repair: for autologous hemoconcentration containing hematoblastic blood plasma, add thrombin of beef and calcium chloride gel, implant defective region to repair, shortcoming: need sterile working, environment, personnel requirement are higher, operating process there is no standardized program, and thrombin of beef there was added immunogenicity risk.
Therefore, being badly in need of a kind of easy to operate clinically, the notable and stable product of regeneration effect is for repair of cartilage.
Owing to articular cartilage does not has blood supply, self repair ability extreme difference, articular cartilage damage caused by wound, osteoarthritis and defect are difficult problems for clinical treatment.In recent years, application gene technology and Method of Tissue Engineering treat the two big focuses that articular cartilage damage is research.Organizational project passes through dimensional culture, and simulation vivo biodistribution and mechanical environment carry out constructing function tissue engineering bone/cartilage.Building during organization engineered cartilage, cytoskeleton is that cell provides a stable three-D space structure, and keeps enough porosity, carries out mass exchange, growth metabolism for it, thus guides Subchondral drilling.The biomaterial of syringeability has and uniformly can mix with cell, and it is fitting to connection between repair tissue, the easy feature of plastotype and arthroscopic procedures can be passed through, treatment is simply, safely, effectively, meet the requirement of modern minimal invasive operation, therefore, the cartilage tissue engineered product of syringeability should be the developing direction of clinical practice.
Syringeability shell is poly-/and β phosphoglycerol two sodium gel is compound repairs rabbit knee cartilage defect and the intervention effect of Radix Clematidis by planting a different chondrocyte of stopping, disclose the material selecting chitosan, β-phosphoglycerol disodium as synthesis support, be combined the preparation of chondrocyte gel for injectable type C/ β-GP.It comprises the concrete steps that, is dissolved in 60ml0.1mol/l dilute hydrochloric acid solution by 1.2 grams of chitosans, is sufficiently stirred for mixing under room temperature, and high pressure steam sterilization is sterilized 10 minutes, 4 DEG C of Refrigerator stores.2.8 grams of β-phosphoglycerol disodiums are completely dissolved in 5ml deionized water, 0.22 μm filter filtration sterilization, 4 DEG C of Refrigerator stores.
Injectable type C/ β-GP is combined the preparation of chondrocyte gel: the 20g/L chitosan solution prepared and 560g/L β-phosphoglycerol two sodium solution are mixed with 7:1 under condition of ice bath, pH value surveyed by pH meter is 7.2, standby with chondrocyte mixing afterwards, cell concentration is 5 × 1010L-1.The shortcoming of the method is: (1) needs second operation, and operation for the first time is taken from body articular chondrocytes and cultivated in vitro, and cartilage and support are mixed by second time operation again implants defective region；(2) condition of culture is harsh, needs the laboratory of corresponding certification to carry out；(3) infecting and the risk of virus spread is big, need to use complete medium when cultivating, complete medium is contained within animal serum, infects and the risk of virus spread increases.
Summary of the invention
In order to solve above-mentioned technical problem, the invention provides a kind of absorbable cartilage repair systems, this system includes absorbable repair of cartilage support and autologous blood, and can absorb repair of cartilage support is mainly composed of chitosan, glycerophosphate, pH value regulator, osmotic pressure regulator and water.This system is gel at Human Physiology temperature (37 DEG C), repairs in cartilage defect, can realize the reparation of cartilaginous tissue.
In the main component of absorbable repair of cartilage support, chitosan 0.1%～5%, glycerophosphate 0.1%～10%, pH value regulator 0.01%～5%, osmotic pressure regulator 0.001%～2%, surplus is water (above ratio is mass concentration percentage ratio).
In above-mentioned raw material, its deacetylation of chitosan should be not less than 70%.Chitosan (Chitosan) has another name called chitosan；Chitosan；Chitosan；Chitan；Chitosan, for Crusta Penaeus seu Panulirus, Carapax Eriocheir sinensis extract chitin through deacetylated product.The chitosan biological compatibility is good, nontoxic, non-immunogenicity, has many application at medical treatment, pharmacy, cosmetics and field of food.In the medical field, chitosan has hemostasis, promotes wound healing, prevents pathologic adhesion, antibacterial, antifungal and antitumor performance.
Chitosan can be degraded into glucosamine and acetylglucosamine by some certain enzyme of such as lysozyme, eventually enters in the carbohydrate metabolism circulation of human body, is a kind of good absorbable biological macromole.
Chitosan can be used for stopping blooding, and chitosan is positively charged macromolecular polysaccharide, and the anion binding of Surface of Erythrocytes makes red cell agglutination, activates platelet aggregation, activated thrombin simultaneously, thus reaches the purpose of quick-acting haemostatic powder.
The glycerophosphate of compositions of the present invention is the one or more combination of sodium glycerophosphate, potassium glycerinophosphate, calcium glycerophosphate, ferric glycerylphosphate.Glycerophosphate is one of main component of cell wall teichoic acid, can be as human body phosphorus supplement, in order to meet the human body every day of the needs to phosphorus.Phosphoglycerol participates in the formation of sclerotin, participates in the composition of cell membrane with phospholipid form, and phosphorus is relevant with the enzymatic activity in many metabolism simultaneously, and the effect in energy metabolism is most important.
Composition pH of the present invention should be between 6.0～8.0, and pH value regulator is hydrochloric acid, sulphuric acid, phosphoric acid, nitric acid, acetic acid, citric acid, succinic acid, tartaric one or more combination.
Compositions osmotic pressure of the present invention should be 160mOsm/Kg～360mOsm/Kg, and osmotic pressure regulator is the one or more combination of sodium chloride, disodium hydrogen phosphate, sodium dihydrogen phosphate, calcium chloride, potassium chloride, sodium lactate, sodium acetate.
Compositions of the present invention can form a kind of stable hydrogel after preparation, and hydrogel is the macromolecular network of high degree of hydration, and the macromolecular network of said composition is chitosan.This preparation method comprises the following steps: dissolve the chitosan in acid solution, glycerophosphate is dissolved in the water, the two is mixed to form composite solution, by the pH regulator of composite solution to 6.0～8.0, add osmotic pressure regulator and the osmotic pressure of described composite solution is regulated to 160mOsm/Kg～360mOsm/Kg.The composite solution being thusly-formed is absorbable repair of cartilage support, after itself and autologous blood mix, can form stable hydrogel being warmed up to 37 DEG C, and this hydrogel is absorbable cartilage repair systems of the present invention.
The concrete compound method of recovery support is, preparation mass percent is 0.1%～5% chitosan solution, is placed in 0.1M～10M acid solution by above-mentioned chitosan solution, obtains mixed solution；
Preparation mass percent is 0.1%～5%0.1%～20% phosphoglycerol saline solution；
Being mixed by the volume ratio of 0.1～10:1 with phosphoglycerol saline solution by mixed solution, regulate pH to 6.0～8.0, adding osmotic pressure regulator and adjusting osmotic pressure is 160mOsm/Kg～360mOsm/Kg, obtains absorbable cartilage frame.
In repair system, absorbable cartilage frame is 1-20:1 with the volume ratio of autologous blood, after they combine, and gel at Human Physiology temperature 37 DEG C, repair in cartilage defect, it is achieved the reparation of cartilaginous tissue.
The absorbable cartilage repair systems of the present invention implants person joint's damage location, forms stable hydrogel under Human Physiology temperature conditions, has the effect promoting that cartilaginous tissue is repaired.
Absorbable cartilage repair systems of the present invention utilizes chitosan to form support gel after caning absorb repair of cartilage support and the mixing of autologous blood, it is implanted directly into damaged cartilage region, utilize the various somatomedin of autologous blood and chitosan Induction of chondrocytes and adhere to the effect of chondrocyte, accelerating reparation and the regeneration of cartilaginous tissue, one time Minimally Invasive Surgery can complete.Solve articular cartilage there is no blood supply, provide for chondrocyte growth and stablize three dimensions and Minimally Invasive Surgery completes many clinical problem such as repair of cartilage.
Absorbable repair of cartilage support has the advantage that for repair of cartilage
The most aseptic, apyrogeneity, non-immunogenicity, biocompatibility is good, and degradable absorbs；
2. utilize the chitosan can Induction of chondrocytes adhere to its surface as support；
3. chitosan and the mixing of autologous blood can form stable support gel, implant Cranial defect regional stability reliable, and the somatomedin in autologous blood can promote the growth of chondrocyte, shortens the formation time of cartilaginous tissue；
4. a Minimally Invasive Surgery can complete repair of cartilage, saves doctor's time, reduces the misery of patient；
5. operating process is simple, and it is little that probability is infected in microorganism；
6., relative to the method provided in background technology, the present invention is absorbable repair of cartilage support and the mixing of autologous blood, it is only necessary to once perform the operation, it is not necessary to In vitro culture, there is not immunogenicity and infects and the risk of affecting animals virus.
Detailed description of the invention
Below by specific embodiment, the present invention is further elaborated, in order to those skilled in the art know more about the present invention, but and are not so limited the present invention.
Embodiment 1
Absorbable repair of cartilage support preparation method step specific as follows:
The chitosan of preparation 1% is in 0.2mol/L acetum, the phosphoglycerol aqueous solutions of potassium of preparation 10%, weigh 1% chitosan solution 20g, drip 10% β-phosphoglycerol potassium 12.0g, stirring while adding, being 7.54 with vinegar acid for adjusting pH after dropping, being subsequently adding sodium acetate and regulating osmotic pressure in right amount is 300 mOsm/Kg, continues stirring 30min.This mixed liquor 2ml, adds 1ml rat blood, after mix homogeneously, is placed in the water bath of 37 DEG C, gets final product the absorbable repair of cartilage support of gel.
Embodiment 2
The chitosan of preparation 2% is in 0.1mol/L hydrochloric acid solution, the phosphoglycerol sodium water solution of preparation 7.5%, weigh 2% chitosan solution 40g, drip 7.5% β-phosphoglycerol sodium 36g, stirring while adding, being 6.56 with salt acid for adjusting pH after dropping, being subsequently adding sodium chloride regulation osmotic pressure is 310 mOsm/Kg, continues stirring 30min.This mixed liquor 5ml, adds 1ml rabbit blood, after mix homogeneously, is placed in the water bath of 37 DEG C, can absorb repair of cartilage support by gel.
Embodiment 3
Preparation phosphate buffer: take disodium hydrogen phosphate 71.64g, be dissolved in water into 1000ml, as solution A.Separately take sodium dihydrogen phosphate 27.60g, be dissolved in water into 1000ml, as second liquid.Taking solution A 81ml and second liquid 19ml, mixing, regulation pH value is to 7.3.
The chitosan of preparation 4% is in 0.1mol/L phosphoric acid solution, the phosphoglycerol sodium water solution of preparation 15%, weigh 4% chitosan solution 30g, drip 15% β-phosphoglycerol sodium 25g, stirring while adding, being 6.56 with phosphorus acid for adjusting pH after dropping, adding above phosphate buffer regulation osmotic pressure is that 302 mOsm/Kg continue stirring 30min.This mixed liquor 10ml, adds 1ml human blood, after mix homogeneously, is placed in the water bath of 37 DEG C, can absorb repair of cartilage support by gel.
Embodiment 4
Prepared by rat cartilage cell: take 4～5 week old, the SD rat of body weight 185～200, disconnected neck is put to death, cut bilateral hip and knee joints cartilage under aseptic condition, put into culture dish, immerse and L-DMEM culture medium rinses for several times, shred, the trypsinization of 0.25%, after 80 eye mesh screens filter, adds L-DMEM complete medium and cultivates.When cell is fused to monolayer, with trypsinization and carry out passage.
Example 1 mixed solution and fresh rat serum are in the ratio mix homogeneously of 1:1, by above-mentioned rat cartilage cell suspendible wherein, add 3 holes in 6 well culture plates, every hole 1ml, after being put in 37 DEG C of incubator 15min formation gels, every hole adds 4mlL-DMEM complete medium and cultivates, and remaining 3 hole is directly added into rat cartilage cell liquid 1ml and 4mlL-DMEM complete medium is cultivated.Result shows, the sample chondrocyte doubling time adding embodiment 1 mixed solution and fresh rat serum is 5 days, and the sample chondrocyte doubling time not added is 7 days, illustrates that the present composition has the effect promoting cartilage-derived growth.
Embodiment 5
By longitudinal cut inside adult rabbits knee joint, successively cutting skin, subcutaneous tissue and joint capsule, tractive patella makes to dislocate laterally, appear femoral bone pulley articular surface, with the drill bit of diameter 4mm ectocondyle in right femur, in fl, condyle produces diameter 0.6cm, degree of depth 0.2cm holostrome thickness cartilage defect model.
Example 1 mixed solution 0.5ml, adds fresh Sanguis Leporis seu oryctolagi 2.5ml, mix homogeneously, by surgical operation implantation in rabbit back leg cartilage defect model district, waits that 15min i.e. forms complete gel, sew up a wound.Again observing cartilage defect district after 6 weeks, show flat smooth, be difficult to differentiate between with surrounding normal cartilage, color and luster is porcelain white.Using Wakitani histological score method to mark cartilage defect district, after 6 weeks as a result, the scoring of rabbit back leg cartilage defect model district is 4.1 ± 0.8.
Embodiment 6
By longitudinal cut inside adult sheep knee joint, successively cut skin, subcutaneous tissue and joint capsule, tractive patella makes to dislocate laterally, appear femoral bone pulley articular surface, with the drill bit of diameter 10mm ectocondyle in right femur, in fl, condyle produces diameter 0.8cm, degree of depth 0.4cm holostrome thickness cartilage defect model.
Example 3 mixed solution 0.5ml, adds fresh Sanguis caprae seu ovis 3.5ml, mix homogeneously, implants sheep defects of knee model district by surgical operation, waits that 15min i.e. forms complete gel, sews up a wound.Within 6 months, again observing cartilage defect district, show flat smooth, be difficult to differentiate between with surrounding normal cartilage, color and luster is porcelain white.Histological observation finds, after 6 months, chondrocyte volume is relatively big, and quantity is more, it is seen that columnar arrangement, close with surrounding normal cartilage tissue, and repair tissue and surrounding cartilage are integrated good.
Claims (10)
1. an absorbable cartilage repair systems, it is characterised in that described repair system mainly includes absorbable cartilage frame.
2. a kind of absorbable cartilage repair systems as claimed in claim 1, it is characterised in that the main component of described absorbable repair of cartilage support includes chitosan, glycerophosphate, pH value regulator, osmotic pressure regulator and water.
3. a kind of absorbable cartilage repair systems as claimed in claim 2, it is characterized in that, the mass percent of the main component of described absorbable repair of cartilage support is: chitosan 0.1%～5%, glycerophosphate 0.1%～10%, pH value regulator 0.01%～5%, osmotic pressure regulator 0.001%～2%, surplus is water.
4. a kind of absorbable cartilage repair systems as claimed in claim 2, it is characterised in that its deacetylation of described chitosan is not less than 70%.
5. a kind of absorbable cartilage repair systems as claimed in claim 2, it is characterised in that described glycerophosphate is at least one in sodium glycerophosphate, potassium glycerinophosphate, calcium glycerophosphate, ferric glycerylphosphate.
6. a kind of absorbable cartilage repair systems as claimed in claim 2, it is characterised in that the pH value of described absorbable repair of cartilage support is 6.0～8.0；Described pH value regulator be hydrochloric acid, sulphuric acid, phosphoric acid, nitric acid, acetic acid, citric acid, succinic acid, tartaric at least one.
7. a kind of absorbable cartilage repair systems as claimed in claim 2, it is characterized in that, the osmotic pressure of described absorbable repair of cartilage support is 160mOsm/Kg～360mOsm/Kg, and osmotic pressure regulator is at least one in sodium chloride, disodium hydrogen phosphate, sodium dihydrogen phosphate, calcium chloride, potassium chloride, sodium lactate, sodium acetate.
8. the one as according to any one of claim 1-7 can absorb cartilage repair systems, it is characterised in that described repair system also includes that autologous blood, described absorbable cartilage frame are 1-20:1 with the volume ratio of autologous blood.
9. the preparation method of a kind of absorbable cartilage repair systems as according to any one of claim 1-7, it is characterised in that the preparation method of described absorbable cartilage frame includes following step:
Preparation mass percent is 0.1%～5% chitosan solution, is placed in 0.1M～10M acid solution by above-mentioned chitosan solution, obtains mixed solution；
Preparation mass percent is 0.1%～5%0.1%～20% phosphoglycerol saline solution；
Being mixed by the volume ratio of 0.1～10:1 with phosphoglycerol saline solution by mixed solution, regulate pH to 6.0～8.0, adding osmotic pressure regulator and adjusting osmotic pressure is 160mOsm/Kg～360mOsm/Kg, obtains absorbable cartilage frame.
The preparation method of a kind of absorbable cartilage repair systems the most as described in claim 9, it is characterized in that, described repair of cartilage support is combined with the volume ratio of 1-20:1 with autologous blood, gel at Human Physiology temperature 37 DEG C, repair in cartilage defect, it is achieved the reparation of cartilaginous tissue.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107349478A (en) * | 2017-06-08 | 2017-11-17 | 西安交通大学 | Sodium glycero-phosphate/chitosan/polyethylene glycol sustained-release gel and its production and use |
| CN110464705A (en) * | 2019-09-03 | 2019-11-19 | 南通大学 | A kind of temperature sensitive type aquagel contains cell material and preparation method and application |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1471412A (en) * | 2000-06-29 | 2004-01-28 | ����ϳɼ������ô�˾ | Composition and method for the repair and regeneration of cartilage and other tissues |
| CN1593385A (en) * | 2004-06-22 | 2005-03-16 | 天津大学 | Gel capable of injecting temperature sensitive complex and its preparation method |
-
2016
- 2016-06-13 CN CN201610410453.0A patent/CN105944149A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1471412A (en) * | 2000-06-29 | 2004-01-28 | ����ϳɼ������ô�˾ | Composition and method for the repair and regeneration of cartilage and other tissues |
| CN1593385A (en) * | 2004-06-22 | 2005-03-16 | 天津大学 | Gel capable of injecting temperature sensitive complex and its preparation method |
Non-Patent Citations (1)
| Title |
|---|
| 马勇 等: ""可注射性壳聚糖/β-甘油磷酸二钠凝胶复合同种异体软骨细胞修复兔膝关节软骨缺损及威灵仙的干预效应"", 《中国组织工程研究与临床康复》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107349478A (en) * | 2017-06-08 | 2017-11-17 | 西安交通大学 | Sodium glycero-phosphate/chitosan/polyethylene glycol sustained-release gel and its production and use |
| CN110464705A (en) * | 2019-09-03 | 2019-11-19 | 南通大学 | A kind of temperature sensitive type aquagel contains cell material and preparation method and application |
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Application publication date: 20160921 |