CN105941889A - Method using cynomorium songaricum powder to cultivate sheep and method for determining mutton quality of cultivated sheep - Google Patents

Method using cynomorium songaricum powder to cultivate sheep and method for determining mutton quality of cultivated sheep Download PDF

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CN105941889A
CN105941889A CN201610346437.XA CN201610346437A CN105941889A CN 105941889 A CN105941889 A CN 105941889A CN 201610346437 A CN201610346437 A CN 201610346437A CN 105941889 A CN105941889 A CN 105941889A
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sheep
mensuration
meat
caprae seu
seu ovis
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双全
柰如嘎
额尔敦木图
嘎拉***
杨君
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Inner Mongolia Agricultural University
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N5/00—Analysing materials by weighing, e.g. weighing small particles separated from a gas or liquid
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/02—Food
    • G01N33/12—Meat; Fish
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N5/00—Analysing materials by weighing, e.g. weighing small particles separated from a gas or liquid
    • G01N5/04—Analysing materials by weighing, e.g. weighing small particles separated from a gas or liquid by removing a component, e.g. by evaporation, and weighing the remainder
    • G01N5/045—Analysing materials by weighing, e.g. weighing small particles separated from a gas or liquid by removing a component, e.g. by evaporation, and weighing the remainder for determining moisture content

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Abstract

The invention discloses a method using cynomorium songaricum powder to cultivate sheep and a method for determining the mutton quality of the cultivated sheep and belongs to the technical field of poultry cultivation and determining. The method using the cynomorium songaricum powder to cultivate sheep includes cultivating healthy sheep by using hay spread with the cynomorium songaricum powder as feed. The method for determining the mutton quality of the cultivated sheep includes determining mutton amino acid and determining mutton fatty acid composition. By the method using the cynomorium songaricum powder to cultivate the sheep and the method for determining the mutton quality of the cultivated sheep, mutton quality is increased, the mutton quality can be analyzed precisely, and the defects that the mutton quality of traditional sheep cannot be determined during cultivation and the mutton quality of the traditional sheep is low are overcome.

Description

A kind of method utilizing Herba Cynomorii powder cultivation sheep and the assay method of quality of mutton thereof
Technical field
The invention belongs to poultry cultivating technology, especially with a kind of method utilizing Herba Cynomorii powder cultivation sheep and Carnis caprae seu ovis product thereof The assay method of matter is relevant.
Background technology
In recent years, along with raising and the enhancing of health perception of people's living standard, consumer to the demand of meals by Gradually by quantity to mass shift.Carnis caprae seu ovis, because of cholesterol succulence relatively low, nutritious, delicious contained by it, is counted as a kind of preferable Health food.Different feedstuff compositions can affect animal feed intake and physiological metabolism function, and then affects meat quality.Raise Feed well-fed feedstuff, sheep can be allowed preferably to grow, moreover it is possible to avoid the generation of a lot of disease.Applicant research is opened Send out and a kind of utilized the Herba Cynomorii powder cultivation method of sheep and the assay method of quality of mutton thereof, it is intended to overcome that quality of mutton exists is scarce Fall into.
Summary of the invention
The problem existed according to above-mentioned background technology, the purpose of the present invention aims to provide the utilization lock that a kind of quality of mutton is good The method of sun powder cultivation sheep and the assay method of quality of mutton thereof.
To this end, the present invention is by the following technical solutions: a kind of method utilizing Herba Cynomorii powder cultivation sheep, it is characterized in that, choose Healthy anosis sheep cultivates with the Radix Glycyrrhizae sprinkled with Herba Cynomorii powder for feedstuff.
A kind of assay method of Carnis caprae seu ovis utilizing Herba Cynomorii powder cultivation sheep, is characterized in that, including the amino acid whose mensuration of Carnis caprae seu ovis and The mensuration of Carnis caprae seu ovis fatty acid composition,
Amino acid whose mensuration, step one: weigh appropriate amount of sample, after drying to constant weight in 65 DEG C of baking ovens, by dewatering and defatting Sample accurately weighs 30mg after pulverizing, and puts in hydrolysis pipe, adds 6mol/L hydrochloric acid 15mL, vacuum-pumping and sealing；At 110 DEG C Hydrolysis 22-24h in thermostatic drying chamber；Filter to 50mL volumetric flask, and use deionized water constant volume.Drawing 1mL filtrate, decompression is steamed Dry, finally it is settled to certain volume with 0.02mol/L hydrochloric acid；Step 2: use full-automatic amino-acid analyzer to measure；Count respectively Calculate its AAS (AAS), amino acid ratio coefficient (RC), the coefficient of variation (CV) and amino acid ratio coefficient (SRC) equivalent, And its nutritive value is judged；In sheep muscle essential amino acids AAS scoring, RC coefficient, CV coefficient and SRC coefficient respectively by with Lower formula calculates:
SRC=100-CV × 100
The mensuration of Carnis caprae seu ovis fatty acid composition, step one: weigh appropriate meat sample, wrap with filter paper, load Soxhlet extractor, use Absolute ether extracts fat in meat sample, and rotary evaporation removes ether；In the fat extracted, add 25mL volume 1mol/L's NaOH-alcohol mixed solution, is placed in 80 DEG C of heating in water bath backflow 1h, carries out the saponification of fat, again carry out rotating steaming after completing Send out, to remove ethanol；Without can the material of saponification more, the soap of generation is dissolved in 10mL distilled water, adds second Ether 10mL, extracts lower floor with separatory funnel, and this step repeatable is repeatedly until completely removing that do not have can the material of saponification；To carry The aqueous pH values taken is adjusted to 2.0, thus separates out fatty acid from sapond material middle reaches；Then with 20mL Petroleum ether extraction fat Acid, repeats this operation 2 times, and rotary evaporation removes petroleum ether again；
Step 2: the HCl-methanol 30mL adding 4% on the fatty acid extracted heats 30min in 60 DEG C of water-baths；Cold But to room temperature, shaking up, wait to be layered, take its supernatant after being separately added into the normal hexane of 20mL and the distilled water of 5mL, rotary evaporation removes Remove normal hexane, add 10mL normal hexane, take its supernatant after layering and be incorporated in together；Use anhydrous Na2SO4Be dried, seal cold Hide, remain to carry out GC-MS analysis；
Normalization quantitative method is used to calculate the quality hundred that in meat samples, each fatty acid component is shared in its total fatty acids Proportion by subtraction, sheep muscle fat each fatty acid component content calculates as follows:
Finally the sheep different parts meat quality of supplementary feeding Herba Cynomorii and non-supplementary feeding Herba Cynomorii is carried out variance analysis, use DuncanShi Method carries out multiple comparisons, and test data represents with mean+SD (X ± SD).
As technique scheme being supplemented and perfect, present invention additionally comprises techniques below feature.
Described assay method also includes the mensuration of the pH value to Carnis caprae seu ovis, the mensuration of color, the mensuration of percentage of water loss, is water rate Mensuration, the mensuration of cold cuts rate, the mensuration of shearing force, the mensuration of collagen protein, the mensuration of moisture, the survey of thick protein Calmly, the mensuration of the mensuration of crude fat, coarse ash, the mensuration of Carnis caprae seu ovis mineral and the mensuration of Carnis caprae seu ovis vitamin.
Being determined as of described pH value takes Carnis caprae seu ovis longissimus dorsi muscle (LD), biceps femoris (BF), arm triceps muscle (TB) 3 respectively Position meat, goes out a hole with little lancination on muscle cross section, then pH value direct tester inserts trunk flesh in the hole stung out Meat, reading.
Being determined as of described percentage of water loss weighs certain meat sample and is placed in centrifuge tube, is centrifuged with the rotating speed of 1500r/min 30min, takes out meat sample, weighs after blotting surface moisture with worry paper after completing, meat sample percentage of water loss be centrifugal after the moisture that loses with Meat sample weight ratio before centrifugal, sheep muscle percentage of water loss is pressed formula and is calculated
The mensuration direct drying method of described system water rate measures the meat sample moisture obtained,
Being determined as of described cold cuts rate takes longissimus dorsi muscle (LD), biceps femoris (BF), 3 positions of arm triceps muscle (TB) respectively Each about about the 30g of meat, removes and weighs after investing the fat on epimysium, the meat sample weighing up weight is put in steaming and decocting 30min in pot, so Rear taking-up is also cooled to room temperature, claims once its weight after blotting surface moisture with worry paper again, and cold cuts rate computing formula is as follows:
Described shearing force be determined as after meat sample removes surface connective tissue and fat thereof, be cut into 2.5cm thick, weigh and very Empty package, is placed in taking-up after water-bath makes the temperature inside meat sample reach about 80 DEG C and is cooled to room temperature, blot surface with filter paper Moisture, weighs；Then meat sample is put into 4 DEG C of refrigerator overnight, and second day, it is thick that the texture along meat sample is cut into 1cm, tender with muscle Degree meter measures its shear force value.And in each sample test repeatedly, and it is average to choose value work relatively, i.e. can get sheep flesh Meat shear force value.
Being determined as of described collagen protein goes out meat sample and hydroxyproline standard solution at 558nm with spectrophotometric determination Light absorption value.Y-axis is made with the hydroxyproline standard solution light absorption value recorded, dense with the hydroxyproline standard solution corresponding with it Degree is made x-axis and is drawn standard curve, calculates my the upper Hydroxyproline concentration corresponding to light absorption value measured by sheep muscle, substitute into Lower formula can draw hydroxyproline content in sheep muscle
Obtain numerical value is multiplied by after hydroxyproline content by sheep muscle again the conversion coefficient 7.1 of terrestrial animal, the most available Collagen content in sheep muscle.
Use the present invention can reach following beneficial effect: the present invention passes through the Radix Glycyrrhizae sprinkled with Herba Cynomorii powder and develops to spread There is the assay method of the Carnis caprae seu ovis of the sheep that Radix Glycyrrhizae is feedstuff of Herba Cynomorii powder, not only increase the quality of Carnis caprae seu ovis, it is also possible to Carnis caprae seu ovis Quality carries out Accurate Analysis, overcomes the tradition sheep defect that quality cannot be concluded and quality is the highest when cultivation to Carnis caprae seu ovis.
Detailed description of the invention
Embodiment 1: first-selected test Carnis caprae seu ovis used picks up from the right flag of Alashan Area, inner Mongolia Autonomous Region, randomly selects healthy anosis sheep, its Middle test group (totally 3 sheep) is with the Radix Glycyrrhizae sprinkled with Herba Cynomorii powder as feedstuff, and matched group (totally 1 sheep) is with Radix Glycyrrhizae as feedstuff.Every sheep After butchering, take its longissimus dorsi muscle (Longissimus Dorsi, LD), biceps femoris (Biceps Femoris, BF) and arm respectively Triceps muscle (Triceps Brachii, TB) each 500g, is transported to laboratory after putting into sampler bag, labelling, freezing (-20 DEG C) To be measured.
The mensuration of pH value: take longissimus dorsi muscle (LD), biceps femoris (BF), 3 position meat of arm triceps muscle (TB) after government official respectively, Muscle cross section goes out a hole with little lancination, then pH value direct tester is inserted trunk muscle in the hole stung out, reading ?.
The mensuration of color: the full-automatic colour examining colour-difference-metre of yellowish pink employing measures.Generally use Hunter value, L*: represent brightness, L*It is the brightest when=100, L*It is the darkest when=0；a*: represent redness, a*The when of being typically larger than 0, it is worth the biggest then muscle color more Red；b*: represent yellowing, b*The when of being typically larger than 0, it is worth the biggest then muscle color the most yellow；Chromatic value (C value) can be by muscle redness Value (a*) and yellow value degree (b*) be calculated, C value the highest then muscle color is the most bright-coloured, computing formula: C=(a*2+b*2)1/2
The mensuration of percentage of water loss: accurately weigh certain meat sample and be placed in centrifuge tube, is centrifuged with the rotating speed of 1500r/min 30min, takes out meat sample, weighs after blotting surface moisture with worry paper after completing, meat sample percentage of water loss be centrifugal after the moisture that loses with Meat sample weight ratio before centrifugal.Alxa sheep muscle percentage of water loss is pressed formula and is calculated
It is the mensuration of water rate: Alxa sheep muscle is that water rate calculates with formula, and wherein moisture content is with reference to GB/ T5009.3-2010, measures the meat sample moisture obtained with direct drying method.
The mensuration of cold cuts rate: take longissimus dorsi muscle (LD), biceps femoris (BF), 3 position meat of arm triceps muscle (TB) respectively each About about 30g, removes and weighs after investing the fat on epimysium, the meat sample weighing up weight is put in steaming and decocting 30min in pot, then takes Go out and be cooled to room temperature, after blotting surface moisture with worry paper, claiming once its weight again.The cold cuts rate of Alxa sheep muscle calculates public affairs Formula:
The mensuration of shearing force: after meat sample removes surface connective tissue and fat thereof, is cut into 2.5cm thick, weighs and vacuum packet Dress, is placed in taking-up after water-bath makes the temperature inside meat sample reach about 80 DEG C and is cooled to room temperature, blot surface moisture with filter paper, Weigh.Then meat sample is put into 4 DEG C of refrigerator overnight, and second day, it is thick that the texture along meat sample is cut into 1cm, uses tenderness device for meat Measure its shear force value.In the many surveys of each sample several times, and choose value work relatively averagely, i.e. can get Alxa sheep flesh Meat shear force value
The mensuration of collagen protein: with reference to GB/T 9695.23 2008 meat and the assay method of hydroxyproline in meat products, use Spectrophotometric determination goes out meat sample and hydroxyproline standard solution light absorption value at 558nm.With the hydroxyproline standard recorded Solution light absorption value makees y-axis, makees x-axis by the hydroxyproline standard solution concentration corresponding with it and draws standard curve.Calculate me The Hydroxyproline concentration corresponding to light absorption value measured by upper sheep muscle, substitutes into formula and can draw hydroxyl dried meat in Alxa sheep muscle Histidine content
The mensuration of moisture: with reference to GB/T5009.3-2010, measure with direct drying method.
The mensuration of thick protein: with reference to GB/T5009.5-2010, use Kjeldahl nitrogen determination.
The mensuration of crude fat: with reference to GB/T5009.6-2003, measure with soxhlet extraction methods
The mensuration of coarse ash: with reference to GB/T5009.4-2010, measure by high temperature ashing method
The mensuration of Alxa Carnis caprae seu ovis mineral
Potassium, sodium: with reference to GB/T5009.91-2003, use atomic absorption spectroscopy determination.
Calcium: with reference to GB/T5009.92-2003, use atomic absorption spectroscopy determination.
Copper: with reference to GB/T5009.13-2003, use atomic absorption spectroscopy determination.
Zinc: with reference to GB/T5009.14-2003, use atomic absorption spectroscopy determination.
Manganese, ferrum, magnesium: with reference to GB/T5009.90-2003, use atomic absorption spectroscopy determination.
Phosphorus: with reference to GB/T5009.87-2003, measure with molybdenum blue method.
The mensuration of Alxa Carnis caprae seu ovis vitamin
VitAVitE: GB/T 5009.82-2003, uses fluorometric determination.
Vitamin B1: GB/T5009.84-2003, uses fluorometric determination.
Vitamin B2: GB/T5009.85-2003, uses fluorometric determination.
Amino acid whose mensuration, step one: weigh appropriate amount of sample, after drying to constant weight in 65 DEG C of baking ovens, by dewatering and defatting Sample accurately weighs 30mg after pulverizing, and puts in hydrolysis pipe, adds 6mol/L hydrochloric acid 15mL, vacuum-pumping and sealing；At 110 DEG C Hydrolysis 22-24h in thermostatic drying chamber；Filter to 50mL volumetric flask, and use deionized water constant volume.Drawing 1mL filtrate, decompression is steamed Dry, finally it is settled to certain volume with 0.02mol/L hydrochloric acid；Step 2: use full-automatic amino-acid analyzer to measure；Count respectively Calculate its AAS (AAS), amino acid ratio coefficient (RC), the coefficient of variation (CV) and amino acid ratio coefficient (SRC) equivalent, And its nutritive value is judged；In sheep muscle essential amino acids AAS scoring, RC coefficient, CV coefficient and SRC coefficient respectively by with Lower formula calculates:
SRC=100-CV × 100
The mensuration of Carnis caprae seu ovis fatty acid composition, step one: weigh appropriate meat sample, wrap with filter paper, load Soxhlet extractor, use Absolute ether extracts fat in meat sample, and rotary evaporation removes ether；In the fat extracted, add 25mL volume 1mol/L's NaOH-alcohol mixed solution, is placed in 80 DEG C of heating in water bath backflow 1h, carries out the saponification of fat, again carry out rotating steaming after completing Send out, to remove ethanol；Without can the material of saponification more, the soap of generation is dissolved in 10mL distilled water, adds second Ether 10mL, extracts lower floor with separatory funnel, and this step repeatable is repeatedly until completely removing that do not have can the material of saponification；To carry The aqueous pH values taken is adjusted to 2.0, thus separates out fatty acid from sapond material middle reaches；Then with 20mL Petroleum ether extraction fat Acid, repeats this operation 2 times, and rotary evaporation removes petroleum ether again；
Step 2: the HCl-methanol 30mL adding 4% on the fatty acid extracted heats 30min in 60 DEG C of water-baths；Cold But to room temperature, shaking up, wait to be layered, take its supernatant after being separately added into the normal hexane of 20mL and the distilled water of 5mL, rotary evaporation removes Remove normal hexane, add 10mL normal hexane, take its supernatant after layering and be incorporated in together；Use anhydrous Na2SO4Be dried, seal cold Hide, remain to carry out GC-MS analysis；
Normalization quantitative method is used to calculate the quality hundred that in meat samples, each fatty acid component is shared in its total fatty acids Proportion by subtraction, sheep muscle fat each fatty acid component content calculates as follows:
SPSS19.0 statistical software is used data to be added up and processes, to the sheep of supplementary feeding Herba Cynomorii and non-supplementary feeding Herba Cynomorii not Carry out variance analysis with position meat quality, carry out multiple comparisons by DuncanShi method.Test data is with mean+SD (X ± SD) represents.
The measurement result of the present invention is as follows:
(1) supplementary feeding Herba Cynomorii Alxa Carnis caprae seu ovis percentage of water loss, be water rate, cold cuts rate, shear force value, hydroxyproline content and glue Former protein content meansigma methods is respectively 25.66%, 65.78%, 55.85%, 4.40kg, 0.16% and 1.14%；Non-supplementary feeding is locked Sun Alxa Carnis caprae seu ovis percentage of water loss, be water rate, cold cuts rate, shear force value, hydroxyproline content and collagen content meansigma methods Be respectively 29.34%, 62.58%, 55.56%, 4.45kg, 0.18% and 1.31%.
(2) Alxa Carnis caprae seu ovis moisture, crude protein, coarse ash and the crude fat content meansigma methods of supplementary feeding Herba Cynomorii is respectively 75.03%, 20.66%, 1.81% and 1.58%；The Alxa Carnis caprae seu ovis moisture of non-supplementary feeding Herba Cynomorii, crude protein, coarse ash and thick fat Fat content meansigma methods is respectively 74.61%, 20.61%, 1.77% and 1.73%.Alxa Carnis caprae seu ovis mineral content is the richest Richness, potassium content the highest (about 415.55mg/100g), next to that phosphorus content (about 188.88mg/100g).Vitamin A, B1、 B2, E content meansigma methods be respectively 89.68 μ g/100g, 13.43 μ g/100g, 53.25 μ g/100g and 0.19mg/100g.I Kind Carnis caprae seu ovis detects 17 kinds of aminoacid, wherein adult's essential amino acids 7 kinds, baby's essential amino acids 2 kinds altogether；Supplementary feeding Herba Cynomorii In Alxa Carnis caprae seu ovis, total amino acids content is 18.17%, and wherein adult's essential amino acids content is 7.18%；Non-supplementary feeding Herba Cynomorii In Alxa Carnis caprae seu ovis, total amino acids content is 18.26%, and wherein adult's essential amino acids content is 7.08%；Its essential amino acids Close to idealized model, and A wide selection of colours and designs.Alxa Carnis caprae seu ovis has been measured 20 kinds of fatty acids altogether, and by oleic acid (34.49%), Petiolus Trachycarpi Acid (22.79%) and stearic acid (13.91%) are main；Supplementary feeding Herba Cynomorii and non-supplementary feeding Herba Cynomorii Carnis caprae seu ovis total fatty acids in saturated fat Acid content is respectively 41.42% and 42.09%, and monounsaturated fatty acid content is respectively 44.61% and 35.10%, many insatiable hungers It is respectively 10.21% and 11.16% with content of fatty acid.
(3) supplementary feeding Herba Cynomorii and between two groups of Alxa sheep of non-supplementary feeding Herba Cynomorii, color value (a in muscle edible quality* Value, b*Value, C value), percentage of water loss, cold cuts rate, hydroxyproline and collagen content have significantly (P < 0.05) difference, other is without aobvious Write difference (P > 0.05)；Potassium, calcium, copper, phosphorus, sodium, vitamin A and B in nutritional labeling1, valine, isoleucine, threonine, benzene Myristic acid in alanine, histidine, arginine, cystine, tyrosine, total fatty acids, pentadecanoic acid, palmitoleic acid, ten Seven alkanoic acids, heptadecanoic acid monoenoic acid, stearic acid, linolelaidic acid, linoleic acid etc. all have notable (P < 0.05) or pole significant difference (P < 0.01), other is without significant difference (P > 0.05).Additionally, the sheep muscle of supplementary feeding Herba Cynomorii has also been measured several a small amount of, do not mending Raise several fatty acids do not measured in the sheep muscle of Herba Cynomorii: heneicosanoic acid, behenic acid, arachidonic acid, eicosapentaenoic acid And docosahexenoic acid (DHA) (EPA).
(4) between the sheep different parts muscle of supplementary feeding Herba Cynomorii, color value (a in edible quality*Value, b*Value, C value), pH value, It is that water rate, cold cuts rate, shear force value, hydroxyproline and collagen content etc. all have notable (P < 0.05) or pole significant difference (P <0.01), other is without significant difference (P>0.05)；Moisture in nutritional labeling, fat, calcium, zinc, phosphorus, vitamin A, histidine, total Palmic acid in fatty acid, palmitoleic acid, heptadecanoic acid monoenoic acid, stearic acid, linolelaidic acid, arachidonic acid, 20 carbon five Olefin(e) acid and behenic acid content etc. the most all have notable (P<0.05) or pole significant difference (P<0.01), other without significant difference (P> 0.05).Additionally, the sheep longissimus dorsi muscle of supplementary feeding Herba Cynomorii does not records docosahexenoic acid (DHA), and containing a small amount of in biceps femoris With arm triceps muscle.
The ultimate principle of the present invention and principal character and advantages of the present invention have more than been shown and described.The technology of the industry Personnel, it should be appreciated that the present invention is not restricted to the described embodiments, simply illustrating this described in above-described embodiment and description The principle of invention, without departing from the spirit and scope of the present invention, the present invention also has various changes and modifications, and these become Change and improvement both falls within scope of the claimed invention.Claimed scope by appending claims and Equivalent defines.

Claims (9)

1. the method utilizing Herba Cynomorii powder cultivation sheep, it is characterised in that: choose healthy anosis sheep with doing sprinkled with Herba Cynomorii powder Grass cultivates for feedstuff.
2. the assay method of Carnis caprae seu ovis utilizing Herba Cynomorii powder cultivation sheep, it is characterised in that: include the amino acid whose mensuration of Carnis caprae seu ovis and The mensuration of Carnis caprae seu ovis fatty acid composition,
Amino acid whose mensuration, step one: weigh appropriate amount of sample, after drying to constant weight in 65 DEG C of baking ovens, by the sample of dewatering and defatting Accurately weigh 30mg after pulverizing, put in hydrolysis pipe, add 6mol/L hydrochloric acid 15mL, vacuum-pumping and sealing；At the constant temperature of 110 DEG C Hydrolysis 22-24h in drying baker；Filter to 50mL volumetric flask, and use deionized water constant volume.Absorption 1mL filtrate, evaporated under reduced pressure, It is settled to certain volume afterwards with 0.02mol/L hydrochloric acid；Step 2: use full-automatic amino-acid analyzer to measure；Calculate it respectively AAS (AAS), amino acid ratio coefficient (RC), the coefficient of variation (CV) and amino acid ratio coefficient (SRC) are equivalent, and right Its nutritive value judges；In sheep muscle, essential amino acids AAS scoring, RC coefficient, CV coefficient and SRC coefficient are respectively by following public affairs Formula calculates:
SRC=100-CV × 100
The mensuration of Carnis caprae seu ovis fatty acid composition, step one: weigh appropriate meat sample, wrap with filter paper, load Soxhlet extractor, with anhydrous Fat in ether extraction meat sample, rotary evaporation removes ether；The NaOH-second of 25mL volume 1mol/L is added in the fat extracted Mixed alkoxide solution, is placed in 80 DEG C of heating in water bath backflow 1h, carries out the saponification of fat, again carry out rotary evaporation, to remove after completing Remove ethanol；Without can the material of saponification more, the soap of generation is dissolved in 10mL distilled water, adds ether 10mL, Extracting lower floor with separatory funnel, this step repeatable is repeatedly until completely removing that do not have can the material of saponification；The water that will extract Phase pH value is adjusted to 2.0, thus separates out fatty acid from sapond material middle reaches；Then with 20mL Petroleum ether extraction fatty acid, weight This operation multiple 2 times, rotary evaporation removes petroleum ether again；
Step 2: the HCl-methanol 30mL adding 4% on the fatty acid extracted heats 30min in 60 DEG C of water-baths；It is cooled to Room temperature, shakes up after being separately added into the normal hexane of 20mL and the distilled water of 5mL, waits to be layered, and takes its supernatant, and rotary evaporation is just removing Hexane, adds 10mL normal hexane, takes its supernatant and be incorporated in together after layering；Use anhydrous Na2SO4It is dried, seals cold preservation, Remain to carry out GC-MS analysis；
Normalization quantitative method is used to calculate the mass percent that in meat samples, each fatty acid component is shared in its total fatty acids, Sheep muscle fat each fatty acid component content calculates as follows:
Finally the sheep different parts meat quality of supplementary feeding Herba Cynomorii and non-supplementary feeding Herba Cynomorii is carried out variance analysis, enter by DuncanShi method Row multiple comparisons, test data represents with mean+SD (X ± SD).
The assay method of a kind of Carnis caprae seu ovis utilizing Herba Cynomorii powder cultivation sheep the most according to claim 2, it is characterised in that: described Assay method also include the mensuration of the pH value to Carnis caprae seu ovis, the mensuration of color, the mensuration of percentage of water loss, be the mensuration of water rate, cold cuts The mensuration of rate, the mensuration of shearing force, the mensuration of collagen protein, the mensuration of moisture, the mensuration of thick protein, the survey of crude fat Calmly, the mensuration of coarse ash, the mensuration of Carnis caprae seu ovis mineral and the mensuration of Carnis caprae seu ovis vitamin.
The assay method of a kind of Carnis caprae seu ovis utilizing Herba Cynomorii powder cultivation sheep the most according to claim 3, it is characterised in that: described Being determined as of pH value take Carnis caprae seu ovis longissimus dorsi muscle (LD), biceps femoris (BF), 3 position meat of arm triceps muscle (TB) respectively, at flesh Go out a hole with little lancination on meat cross section, then pH value direct tester is inserted trunk muscle in the hole stung out, reading.
The assay method of a kind of Carnis caprae seu ovis utilizing Herba Cynomorii powder cultivation sheep the most according to claim 3, it is characterised in that: described Being determined as of percentage of water loss weighs certain meat sample and is placed in centrifuge tube, is centrifuged 30min with the rotating speed of 1500r/min, takes after completing Go out meat sample, weigh after blotting surface moisture with worry paper, meat sample percentage of water loss be centrifugal after the moisture that loses with centrifugal before meat sample weight Ratio, sheep muscle percentage of water loss press formula calculate
The assay method of a kind of Carnis caprae seu ovis utilizing Herba Cynomorii powder cultivation sheep the most according to claim 3, it is characterised in that: described It is that the mensuration direct drying method of water rate measures the meat sample moisture that obtains,
The assay method of a kind of Carnis caprae seu ovis utilizing Herba Cynomorii powder cultivation sheep the most according to claim 3, it is characterised in that: described Being determined as of cold cuts rate takes longissimus dorsi muscle (LD), biceps femoris (BF) respectively, each about 30g is left for 3 position meat of arm triceps muscle (TB) The right side, removes and weighs after investing the fat on epimysium, the meat sample weighing up weight is put in steaming and decocting 30min in pot, then takes out and cold But to room temperature, claiming once its weight after blotting surface moisture with worry paper again, cold cuts rate computing formula is as follows:
The assay method of a kind of Carnis caprae seu ovis utilizing Herba Cynomorii powder cultivation sheep the most according to claim 3, it is characterised in that: described Shearing force be determined as after meat sample removes surface connective tissue and fat thereof, be cut into 2.5cm thick, weigh and be vacuum-packed, being placed in Water-bath takes out after making the temperature inside meat sample reach about 80 DEG C and is cooled to room temperature, blots surface moisture with filter paper, weighs；So After meat sample put into 4 DEG C of refrigerator overnight, second day, it is thick that the texture along meat sample is cut into 1cm, measures it with tenderness device for meat and cuts Shear value, and in each sample test repeatedly, and it is average to choose value work relatively, i.e. can get sheep muscle shear force value.
The assay method of a kind of Carnis caprae seu ovis utilizing Herba Cynomorii powder cultivation sheep the most according to claim 3, it is characterised in that: described Being determined as of collagen protein goes out meat sample and hydroxyproline standard solution light absorption value at 558nm with spectrophotometric determination, uses The hydroxyproline standard solution light absorption value recorded makees y-axis, makees x-axis by the hydroxyproline standard solution concentration corresponding with it and draws Standard curve, calculates my the upper Hydroxyproline concentration corresponding to light absorption value measured by sheep muscle, substitutes into below equation Draw
Obtain numerical value is multiplied by after hydroxyproline content by sheep muscle again the conversion coefficient 7.1 of terrestrial animal, i.e. can get sheep flesh Collagen content in meat.
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CN110470805A (en) * 2019-08-12 2019-11-19 中国农业科学院农产品加工研究所 Hot fresh mutton determination method
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Publication number Priority date Publication date Assignee Title
CN107064441A (en) * 2017-05-27 2017-08-18 陕西师范大学 The method that quality of mutton is evaluated based on the labelled protein that phosphated peptide section is screened
CN107064441B (en) * 2017-05-27 2019-06-28 陕西师范大学 The method of labelled protein evaluation quality of mutton based on phosphated peptide section screening
CN111051809A (en) * 2017-07-14 2020-04-21 Can科技公司 Method and apparatus for assessing muscle development in an animal
CN111051809B (en) * 2017-07-14 2022-03-08 Can科技公司 Method and apparatus for assessing muscle development in an animal
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