CN105941480A - Sterilizing composite of bacillus atrophaeus and lentinan - Google Patents
Sterilizing composite of bacillus atrophaeus and lentinan Download PDFInfo
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- CN105941480A CN105941480A CN201610495405.6A CN201610495405A CN105941480A CN 105941480 A CN105941480 A CN 105941480A CN 201610495405 A CN201610495405 A CN 201610495405A CN 105941480 A CN105941480 A CN 105941480A
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- China
- Prior art keywords
- lentinan
- bacillus cereus
- atrophy bacillus
- bactericidal composition
- tobacco
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Pest Control & Pesticides (AREA)
- Wood Science & Technology (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a sterilizing composite of bacillus atrophaeus and lentinan. The sterilizing composite comprises effective ingredients of bacillus atrophaeus and lentinan, wherein the content of bacillus atrophaeus is one hundred million to five billion CFU/g; the mass percent of lentinan is (0.1-1.5)%. The sterilizing composite is prepared by compounding bacillus atrophaeus and lentinan and can be used for preventing and treating tobacco bacterial wilt disease and virus disease of tobacco, and the sterilizing composite has good preventing and treating effects; meanwhile, the sterilizing composite can improve the tobacco resistance and improve the tobacco yield and quality, and moreover, the sterilizing composite is safe to human, animal, beneficial organisms and environment.
Description
Technical field
The invention belongs to disinfectant use in agriculture field, be specifically related to a kind of atrophy bacillus cereus and Lentinus Edodes
The bactericidal composition of polysaccharide.
Background technology
Atrophy bacillus cereus (Bacillus atrophaeus) is once called as Bacillus subtilis endophyticus.
Smith et al. is found that atrophy bacillus cereus original strain NRS-1221A in nineteen fifty-two, should
Bacterial strain can produce melanin in the culture medium containing tyrosine, is referred to according to this characteristic
In Bacillus subtilis endophyticus novel species.1989, Nakamura proposed hay spore
Bacillus var. niger is renamed as atrophy bacillus cereus, to be different from bacillus subtilis.Atrophy bud
Spore bacillus can produce spore and resist poor environment, resistance, is above temperature environment at 80 DEG C
In still can survive, in agricultural, industry, medical treatment, health field has broad application prospects.
Lentinan be a kind of with β-D (1 → 3) glucosan residue as main chain, side chain is (1 → 6)
The glucosan of glucosan residue, is the effective active composition extracted from mushroom fruiting body, aligns
Often body there is no immunologic enhancement, but can make lotus tumor formula or the immunne response of metainfective body
It is improved, is widely used to field of medicaments and field of food at present.In agriculture field, mushroom
Proteoglycan has developed counterpoison 1, antitoxin multiple biological pesticide preparation kinds such as rich,
For preventing and treating the various crop disease such as tomato virus disease, stripe disease, simultaneously can be notable
Improving crop anti-adversity, production-increasing function is obvious.
Using compound to atrophy bacillus cereus and lentinan as antibacterial, it is used for preventing and treating Nicotiana tabacum L. green grass or young crops withered
Disease, double controls tobacco virus, has no report.
Summary of the invention
The present invention uses dilution-plate method separation of bacterial from soil sample, utilizes flat board face-off method,
Measure its inhibitory activity to Pseudomonas solanacearum, it is thus achieved that there is the antibacterial of notable fungistatic effect.
Identified by the sequence similarity of kind horizontal conservative gene gyrB, and named YC21.
The present invention is based on atrophy Bacillus strain YC21, it is provided that a kind of containing effectively becoming
Divide atrophy bacillus cereus YC21 and the disinfecting compositions of lentinan, this bactericidal composition
Tobacco bacterial wilt is had good prevention effect, double controls tobacco virus, enhance cigarette simultaneously
Grass resistance, improves Nicotiana tabacum L. yield and quality.
Technical scheme is as follows:
Use screening obtain atrophy bacillus cereus YC21 with and lentinan compound sterilized
Compositions, wherein atrophy bacillus cereus YC21 content is (10～50) hundred million CFU/g, Lentinus Edodes
The mass percent of polysaccharide is (0.1～1.5) %.
Described bactericidal composition, most preferred atrophy bacillus cereus YC21 content is 1,000,000,000
CFU/g, the mass percent of lentinan is (0.5～1.2) %.
Described bactericidal composition, its formulation is aqueous suspension agent, wettable powder.Use
Agricultural bactericidal agent aid include surfactant, dispersant, thickening agent, defoamer, filler
Etc. each analog assistant, the surfactant of employing can be sodium lignin sulfonate, Oleum Ricini polyoxy second
In alkene ether, fatty alcohol-polyoxyethylene ether, calcium lignosulfonate, sodium methylene bis-naphthalene sulfonate one
Plant or multiple；The thickening agent used is xanthan gum；The defoamer used can be organic silicon defoamer；
The filler used can be one or more in white carbon, Kaolin, attapulgite etc..
Described bactericidal composition is used for preventing and treating tobacco bacterial wilt, double tobacco virus of controlling, preventing and treating
Respond well, enhance Nicotiana tabacum L. resistance simultaneously, improve Nicotiana tabacum L. yield and quality.
Described atrophy bacillus cereus (Bacillus atrophaeus) YC21 bacterial strain is in 2015
On June 11, in is by China typical culture collection center (CCTCC) preservation (depositary institution ground
Location: Wuhan, China Wuhan University；Postcode: 430072；Phone: 02768754052.),
Preserving number is CCTCC NO:M2015364.
The present invention, by gathering a large amount of soil samples in tobacco rhizosphere, uses dilution-plate method, point
Separate out series biocontrol bacterial strain, by the antimicrobial spectrum determination test of system, filter out green grass or young crops withered false single
Born of the same parents bacterium has the atrophy Bacillus strain YC21 of notable antagonism.Employing screening is obtained
Atrophy bacillus cereus YC21 with and lentinan compound obtain bactericidal composition, be used for preventing and treating
Tobacco bacterial wilt, double tobacco virus of controlling, prevention effect is good, enhances Nicotiana tabacum L. degeneration-resistant simultaneously
Property, improve Nicotiana tabacum L. yield and quality.
The bactericidal composition wherein effective ingredient that the present invention provides is biology or biogenic pesticide,
Chemical pesticide can be substituted for tobacco bacterial wilt and the preventing and treating of virosis, this bactericidal composition cost
Low, prevention effect is efficiently and environmental protection.
Detailed description of the invention:
Below in conjunction with embodiment, the invention will be further described, but the essence of the present invention does not limit
In following embodiment.
Embodiment 1
The preparation method of 1000000000 CFU/g atrophy bacillus cereus 0.5% lentinan aqueous suspension agents: by matter
Amount percentage ratio (except atrophy bacillus cereus).
Preparation method: according to formula, by above component (except atrophy bacillus cereus) in proportion
Weigh, add ball mill ball milling 30 minutes, filter, suction dispersion tank high speed shear 3 minutes
Being fully ground by sand mill, control solid constituent particle diameter, within 2 microns, grinds knot
Shu Hou, weighs and introduces atrophy bacillus cereus mother solution, stir, and suction dispersion tank is cut at a high speed
Cut 3 minutes, filter, obtain suspending agent product.
Embodiment 2
The preparation method of 1000000000 CFU/g atrophy bacillus cereus 0.8% lentinan aqueous suspension agents: by matter
Amount percentage ratio (except atrophy bacillus cereus).
Preparation method, with embodiment 1.
Embodiment 3
The preparation method of 1000000000 CFU/g atrophy bacillus cereus 1% lentinan aqueous suspension agents: by quality
Percentage ratio (except atrophy bacillus cereus).
Preparation method: with embodiment 1.
Embodiment 4
The preparation method of 1000000000 CFU/g atrophy bacillus cereus 1.2% lentinan aqueous suspension agents: by matter
Amount percentage ratio (except atrophy bacillus cereus).
Preparation method, with embodiment 1.
Embodiment 5
The preparation method of 1000000000 CFU/g atrophy bacillus cereus 1% lentinan wettable powders: by matter
Amount percentage ratio.
Preparation method: then add in above component formula (except atrophy bacillus cereus) ratio
Mixer mix homogeneously laggard row comminution by gas stream, stirs after having pulverized, will be appropriate
Atrophy fermentation of bacillus liquid comminution by gas stream product adsorbs, be dried, sieve can
WP product.
Embodiment 6
The preparation method of 1000000000 CFU/g atrophy bacillus cereus 0.5% lentinan wettable powders: press
Mass percent.
Preparation method, with embodiment 5.
Preparation is measured to tobacco bacterial wilt and the prevention effect of virosis under field:
Experimental program is as follows:
Trial crops Nicotiana tabacum L., kind is cloud and mist 97；Plot area 50, repeats for 4 times, with
Machine arranges, and sets protection row between each process.
Reagent agent, comparison medicament and dosage:
Embodiment 1 to embodiment 6 preparation dosage is 1500g/ hectare, compares medicament 1,000,000,000
CFU/g hundred million atrophy bacillus cereus (YC21) SC preparation dosage is 3000g/ hectare, 1% Lentinus Edodes
Polysaccharide soluble powder agent formulation dosage is 1500g/ hectare, separately sets the blank of spray clear water.
It is stained with root in tobacco in seedling stage to process and the process of Nicotiana tabacum L. grown in field early stage whole strain spray, each per hectare
Spraying amount of liquid medicine is 900 liters.Spraying equipment is WS-16 type knapsack hand sprayer.?
State of an illness radix investigation, field period second time dispenser " Invest, Then Investigate " morbidity in 60 days is carried out before chemicals treatment
Situation, statistics sickness rate also calculates comprehensive disease and refers to and preventive effect.
Investigation method, classification, drug effect calculate and are performed both by National Standard of the People's Republic of China's " cigarette
Grass pest and disease damage classification investigation method " (GB/T23222-2008).
Result of the test:
After table 1 atrophy bacillus cereus lentinan compositions secondary dispenser 60 days, the field control to tobacco bacterial wilt and virosis is imitated
Really
Shown in result of the test (table 1): 6 preparations are executed example and all reached the preventive effect of tobacco bacterial wilt
To more than 45%, the preventive effect of tobacco virus is reached more than 40%.Embodiment 3, embodiment 4,
Embodiment 5 all reaches more than 55% to the preventive effect of tobacco bacterial wilt, the preventive effect to tobacco virus
Reaching more than 50%, embodiment 1, embodiment 2, embodiment 6 are to tobacco bacterial wilt and virus
Sick preventive effect is slightly worse, respectively 50% and more than 45%.
Claims (5)
1. an atrophy bacillus cereus and the bactericidal composition of lentinan, it is characterized in that: the described effective ingredient in bactericidal composition is atrophy bacillus cereus and lentinan, described atrophy bacillus cereus content is (1～50) hundred million CFU/g, and the mass percent of lentinan is (0.1～1.5) %.
Atrophy bacillus cereus the most according to claim 1 and the bactericidal composition of lentinan, it is characterised in that: in described bactericidal composition, atrophy bacillus cereus content is 1,000,000,000 CFU/g, and the mass percent of lentinan is (0.5～1.2) %.
Atrophy bacillus cereus the most according to claim 1 and the bactericidal composition of lentinan, it is characterised in that: the described entitled YC21 of atrophy bacillus cereus, preservation number is CCTCC NO.M2015364.
Atrophy bacillus cereus the most according to claim 1 and the bactericidal composition of lentinan, it is characterised in that: described bactericidal composition dosage form is aqueous suspension agent or wettable powder.
The bactericidal composition of atrophy bacillus cereus the most according to claim 1 and lentinan can be used for preventing and treating tobacco bacterial wilt, double controls tobacco virus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610495405.6A CN105941480A (en) | 2016-06-27 | 2016-06-27 | Sterilizing composite of bacillus atrophaeus and lentinan |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610495405.6A CN105941480A (en) | 2016-06-27 | 2016-06-27 | Sterilizing composite of bacillus atrophaeus and lentinan |
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| CN105941480A true CN105941480A (en) | 2016-09-21 |
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| CN201610495405.6A Pending CN105941480A (en) | 2016-06-27 | 2016-06-27 | Sterilizing composite of bacillus atrophaeus and lentinan |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113498792A (en) * | 2021-06-09 | 2021-10-15 | 云南星耀生物制品有限公司 | Application of bacillus subtilis preparation in preventing and treating cruciferae clubroot |
-
2016
- 2016-06-27 CN CN201610495405.6A patent/CN105941480A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113498792A (en) * | 2021-06-09 | 2021-10-15 | 云南星耀生物制品有限公司 | Application of bacillus subtilis preparation in preventing and treating cruciferae clubroot |
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Application publication date: 20160921 |