CN105919106A - Lycium ruthenicum procyanidine oral liquid and preparation method thereof - Google Patents
Lycium ruthenicum procyanidine oral liquid and preparation method thereof Download PDFInfo
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- CN105919106A CN105919106A CN201610368375.2A CN201610368375A CN105919106A CN 105919106 A CN105919106 A CN 105919106A CN 201610368375 A CN201610368375 A CN 201610368375A CN 105919106 A CN105919106 A CN 105919106A
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- Prior art keywords
- lycium ruthenicum
- procyanidin
- ruthenicum murr
- oral liquid
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- 241000169546 Lycium ruthenicum Species 0.000 title claims abstract description 125
- 239000007788 liquid Substances 0.000 title claims abstract description 66
- 238000002360 preparation method Methods 0.000 title claims abstract description 34
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 45
- 239000000843 powder Substances 0.000 claims abstract description 27
- 108010011485 Aspartame Proteins 0.000 claims abstract description 25
- 239000000605 aspartame Substances 0.000 claims abstract description 25
- IAOZJIPTCAWIRG-QWRGUYRKSA-N aspartame Chemical compound OC(=O)C[C@H](N)C(=O)N[C@H](C(=O)OC)CC1=CC=CC=C1 IAOZJIPTCAWIRG-QWRGUYRKSA-N 0.000 claims abstract description 25
- 235000010357 aspartame Nutrition 0.000 claims abstract description 25
- 229960003438 aspartame Drugs 0.000 claims abstract description 25
- 239000000243 solution Substances 0.000 claims abstract description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 21
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 claims abstract description 20
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000000811 xylitol Substances 0.000 claims abstract description 20
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 claims abstract description 20
- 235000010447 xylitol Nutrition 0.000 claims abstract description 20
- 229960002675 xylitol Drugs 0.000 claims abstract description 20
- 238000010992 reflux Methods 0.000 claims abstract description 16
- 238000000034 method Methods 0.000 claims abstract description 12
- 239000003755 preservative agent Substances 0.000 claims abstract description 11
- 230000002335 preservative effect Effects 0.000 claims abstract description 11
- CWEZAWNPTYBADX-UHFFFAOYSA-N Procyanidin Natural products OC1C(OC2C(O)C(Oc3c2c(O)cc(O)c3C4C(O)C(Oc5cc(O)cc(O)c45)c6ccc(O)c(O)c6)c7ccc(O)c(O)c7)c8c(O)cc(O)cc8OC1c9ccc(O)c(O)c9 CWEZAWNPTYBADX-UHFFFAOYSA-N 0.000 claims description 102
- 229920002414 procyanidin Polymers 0.000 claims description 102
- XFZJEEAOWLFHDH-UHFFFAOYSA-N (2R,2'R,3R,3'R,4R)-3,3',4',5,7-Pentahydroxyflavan(48)-3,3',4',5,7-pentahydroxyflavan Natural products C=12OC(C=3C=C(O)C(O)=CC=3)C(O)CC2=C(O)C=C(O)C=1C(C1=C(O)C=C(O)C=C1O1)C(O)C1C1=CC=C(O)C(O)=C1 XFZJEEAOWLFHDH-UHFFFAOYSA-N 0.000 claims description 101
- MOJZMWJRUKIQGL-FWCKPOPSSA-N Procyanidin C2 Natural products O[C@@H]1[C@@H](c2cc(O)c(O)cc2)Oc2c([C@H]3[C@H](O)[C@@H](c4cc(O)c(O)cc4)Oc4c3c(O)cc(O)c4)c(O)cc(O)c2[C@@H]1c1c(O)cc(O)c2c1O[C@@H]([C@H](O)C2)c1cc(O)c(O)cc1 MOJZMWJRUKIQGL-FWCKPOPSSA-N 0.000 claims description 101
- HGVVOUNEGQIPMS-UHFFFAOYSA-N procyanidin Chemical compound O1C2=CC(O)=CC(O)=C2C(O)C(O)C1(C=1C=C(O)C(O)=CC=1)OC1CC2=C(O)C=C(O)C=C2OC1C1=CC=C(O)C(O)=C1 HGVVOUNEGQIPMS-UHFFFAOYSA-N 0.000 claims description 101
- 239000000047 product Substances 0.000 claims description 39
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 36
- 239000003208 petroleum Substances 0.000 claims description 17
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 claims description 16
- 239000000706 filtrate Substances 0.000 claims description 16
- 239000004302 potassium sorbate Substances 0.000 claims description 16
- 235000010241 potassium sorbate Nutrition 0.000 claims description 16
- 229940069338 potassium sorbate Drugs 0.000 claims description 16
- 239000000284 extract Substances 0.000 claims description 14
- 230000001954 sterilising effect Effects 0.000 claims description 12
- 238000004659 sterilization and disinfection Methods 0.000 claims description 12
- 238000001914 filtration Methods 0.000 claims description 11
- 239000000203 mixture Substances 0.000 claims description 11
- 239000012535 impurity Substances 0.000 claims description 10
- 238000004090 dissolution Methods 0.000 claims description 9
- 239000011259 mixed solution Substances 0.000 claims description 9
- 238000002156 mixing Methods 0.000 claims description 9
- 239000011347 resin Substances 0.000 claims description 9
- 229920005989 resin Polymers 0.000 claims description 9
- 239000006228 supernatant Substances 0.000 claims description 9
- 230000000007 visual effect Effects 0.000 claims description 9
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 8
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 8
- 238000001035 drying Methods 0.000 claims description 8
- 239000003480 eluent Substances 0.000 claims description 8
- 238000004108 freeze drying Methods 0.000 claims description 8
- 238000007654 immersion Methods 0.000 claims description 8
- 235000012149 noodles Nutrition 0.000 claims description 8
- JGSARLDLIJGVTE-UHFFFAOYSA-N 3,3-dimethyl-7-oxo-6-[(2-phenylacetyl)amino]-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid Chemical compound O=C1N2C(C(O)=O)C(C)(C)SC2C1NC(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-UHFFFAOYSA-N 0.000 claims description 7
- 238000001816 cooling Methods 0.000 claims description 3
- WQZGKKKJIJFFOK-SVZMEOIVSA-N (+)-Galactose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-SVZMEOIVSA-N 0.000 abstract description 18
- 230000032683 aging Effects 0.000 abstract description 11
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract description 7
- 238000000605 extraction Methods 0.000 abstract description 7
- 230000003712 anti-aging effect Effects 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 5
- 238000010172 mouse model Methods 0.000 abstract description 5
- 230000000050 nutritive effect Effects 0.000 abstract description 4
- 238000010521 absorption reaction Methods 0.000 abstract description 3
- 238000011990 functional testing Methods 0.000 abstract 1
- 238000002347 injection Methods 0.000 abstract 1
- 239000007924 injection Substances 0.000 abstract 1
- 230000003647 oxidation Effects 0.000 abstract 1
- 238000007254 oxidation reaction Methods 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 description 12
- 238000002474 experimental method Methods 0.000 description 11
- 238000013461 design Methods 0.000 description 10
- 230000004044 response Effects 0.000 description 10
- 238000005065 mining Methods 0.000 description 9
- 241000699670 Mus sp. Species 0.000 description 8
- 238000005457 optimization Methods 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 230000009471 action Effects 0.000 description 7
- 239000003205 fragrance Substances 0.000 description 7
- 230000002159 abnormal effect Effects 0.000 description 6
- 230000035943 smell Effects 0.000 description 6
- 238000010254 subcutaneous injection Methods 0.000 description 6
- 239000007929 subcutaneous injection Substances 0.000 description 6
- 235000002710 Ilex cornuta Nutrition 0.000 description 5
- 241001310146 Ilex cornuta Species 0.000 description 5
- 235000010326 Osmanthus heterophyllus Nutrition 0.000 description 5
- 229930014669 anthocyanidin Natural products 0.000 description 5
- 150000001453 anthocyanidins Chemical class 0.000 description 5
- 235000008758 anthocyanidins Nutrition 0.000 description 5
- 238000010298 pulverizing process Methods 0.000 description 5
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 4
- 229930002877 anthocyanin Natural products 0.000 description 4
- 235000010208 anthocyanin Nutrition 0.000 description 4
- 239000004410 anthocyanin Substances 0.000 description 4
- 150000004636 anthocyanins Chemical class 0.000 description 4
- HEBKCHPVOIAQTA-NGQZWQHPSA-N d-xylitol Chemical compound OC[C@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-NGQZWQHPSA-N 0.000 description 4
- 229940100688 oral solution Drugs 0.000 description 4
- 229910052700 potassium Inorganic materials 0.000 description 4
- 239000011591 potassium Substances 0.000 description 4
- 241001106041 Lycium Species 0.000 description 3
- 244000173166 Pyrus ussuriensis Species 0.000 description 3
- 235000011572 Pyrus ussuriensis Nutrition 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 238000003304 gavage Methods 0.000 description 2
- 231100000053 low toxicity Toxicity 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 210000000697 sensory organ Anatomy 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 238000013444 DoE analysis Methods 0.000 description 1
- 229940123457 Free radical scavenger Drugs 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 208000019255 Menstrual disease Diseases 0.000 description 1
- KJTLSVCANCCWHF-UHFFFAOYSA-N Ruthenium Chemical compound [Ru] KJTLSVCANCCWHF-UHFFFAOYSA-N 0.000 description 1
- 241000208292 Solanaceae Species 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- BEFDCLMNVWHSGT-UHFFFAOYSA-N ethenylcyclopentane Chemical compound C=CC1CCCC1 BEFDCLMNVWHSGT-UHFFFAOYSA-N 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 208000019622 heart disease Diseases 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 230000002175 menstrual effect Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- -1 polyphenol compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 235000019353 potassium silicate Nutrition 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 229910052707 ruthenium Inorganic materials 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 230000014860 sensory perception of taste Effects 0.000 description 1
- NTHWMYGWWRZVTN-UHFFFAOYSA-N sodium silicate Chemical compound [Na+].[Na+].[O-][Si]([O-])=O NTHWMYGWWRZVTN-UHFFFAOYSA-N 0.000 description 1
- 235000010199 sorbic acid Nutrition 0.000 description 1
- 229940075582 sorbic acid Drugs 0.000 description 1
- 239000004334 sorbic acid Substances 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 238000005211 surface analysis Methods 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 235000015961 tonic Nutrition 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
- 229960000716 tonics Drugs 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention discloses a lycium ruthenicum procyanidine oral liquid and a preparation method thereof. The oral liquid comprises the following components by mass percent: 2 to 5 percent of lycium ruthenicum procyanidine, 5 to 10 percent of xylitol, 0.03 to 0.05 percent of aspartame, 0.03 to 0.05 percent of a preservative, and 85 to 90 percent of pure water. The preparation method comprises the following steps: adding lycium ruthenicum powder into a 61 percent ethanol solution, with the solid-to-liquid ratio of 1 to 19, through an extracting and preparing process for providing lycium ruthenicum procyanidine, so as perform reflux extraction, and ensuring that the extraction temperature is 47 DEG C. The oral liquid prepared has good oxidation resistance, is fast in absorption, easy to store, and rich in nutritive value, meanwhile is proved through functional tests to have an anti-aging effect on ubacute aging mouse models prepared through hypodermic injection of D-galactose normal saline, obviously enhances the SOD activity in aged model mouse sera, enhances the GSH-PX activity and the GAT activity in the aged model mouse sera, reduces the MDA content, has an obvious healthcare function, and has wide practicability.
Description
Technical field
The invention mainly relates to the manufacture method of a kind of oral liquid, specifically, the present invention relates to
A kind of preparing technical field of Lycium ruthenicum Murr. procyanidin oral liquid.
Background technology
Black Fructus Lycii (Lycium Ruthenium Murr.) is Solanaceae (Solanceae) Lycium
(Lycium L.), is NORTHWEST CHINA area distinctive salt tolerant, drought resisting wild plant, widely distributed,
Aboundresources, " Uigurs medicine will " is recorded, and lycium ruthenicum fruit has kidney tonifying, profit liver, bright
Mesh, stomach invigorating, supplementing the brain, defying age and the effect of stimulating the menstrual flow." Jingzhubencao " is recorded, black Fructus Lycii
Notable for treating heart-heat syndrome, heart disease, menoxenia, menolipsis etc. and drug effect, conduct among the people
Strengthening by means of tonics, depressor are used.
Lycium ruthenicum Murr. contains abundant natural procyanidins, and custom has the title of the king of " procyanidin ",
Procyanidin is the class natural polyphenol compounds being widely present in plant, have water-soluble,
Nontoxic, without the characteristic such as allergy, safety be good, be a kind of natural free radical scavenger and antioxygen
Agent, and also defying age, antitumor, antiinflammatory, anticancer change, prevention cardiovascular and cerebrovascular disease
With the physiological function such as prevention of arterial is atherosis.
Oral liquid has that taking dose is little, it is very fast to absorb, steady quality, easy to carry and use,
The advantages such as easy preservation, are especially suitable for industrialized production.But there is presently no and be specifically designed for Lycium ruthenicum Murr.
Procyanidin produces the application of oral liquid, has extensively for exploitation Lycium ruthenicum Murr. procyanidin oral liquid
General realistic meaning.
Summary of the invention
For having no both at home and abroad about the functional prominent Lycium ruthenicum Murr. procyanidin of specific aim at present
The report of oral liquid, it is contemplated that in providing a kind of functional Lycium ruthenicum Murr. procyanidin oral liquid
And preparation method thereof, by use Lycium ruthenicum Murr. procyanidin as pure natural addictive make black
Fructus Lycii procyanidin oral liquid has good non-oxidizability, absorbs very fast, easily preserves, nutrition
It is worth abundant, is confirmed by functional trial, the Lycium ruthenicum Murr. procyanidin oral liquid of preparation simultaneously
The defying age that D-galactose normal saline subcutaneous injection is prepared subacute aging mouse model is made
With, and it is obviously improved SOD vigor in exhausted mining areas serum, promote exhausted mining areas
In serum, GSH-PX vigor and GAT vigor, reduce MDA content, has significantly guarantor
Health-care function, has wide applicability.
The present invention is achieved through the following technical solutions:
By employing Lycium ruthenicum Murr. procyanidin as pure natural addictive, with Lycium ruthenicum Murr. procyanidin
2-5%, xylitol 5-10%, aspartame 0.03-0.05%, preservative 0.03-0.05%,
Pure water 85-90% is as formula, it is provided that the extraction and preparation technique of Lycium ruthenicum Murr. procyanidin, will
Lycium ruthenicum Murr. powder adds 61% ethanol solution reflux, extract, Extracting temperature with the solid-to-liquid ratio of 1:19
47 DEG C, according to scientific design and optimization technological experiment, the Lycium ruthenicum Murr. procyanidin oral liquid of preparation
There is good non-oxidizability, absorb very fast, easily preserve, rich in nutritive value, pass through simultaneously
Functional trial confirms, the Lycium ruthenicum Murr. procyanidin oral liquid of preparation is to D-galactose physiology salt
Water subcutaneous injection prepares the anti-aging effects of subacute aging mouse model, and is obviously improved aging
SOD vigor in model mice serum, promotes GSH-PX vigor in exhausted mining areas serum
With GAT vigor, reduce MDA content, there is obvious health care, have widely
Practicality.
The present invention specifically provides a kind of Lycium ruthenicum Murr. procyanidin oral liquid, according to percentage by weight
Meter, containing Lycium ruthenicum Murr. procyanidin 2-5%, xylitol 5-10%, aspartame 0.03-0.05%,
Preservative 0.03-0.05%, pure water 85-90%.
The preservative that the present invention uses is preferably potassium sorbate, Heat stability is good, and low toxicity can quilt
Digestion decomposes absorption, noresidue.
Meanwhile, the present invention provides the preparation method of above-mentioned Lycium ruthenicum Murr. procyanidin oral liquid, specifically
Techniques below step is used to prepare:
(1) preparation of Lycium ruthenicum Murr. procyanidin: by Lycium ruthenicum Murr. 50 DEG C of drying, pulverized 60
Mesh sieve, adds 8 times amount petroleum ether immersion 24h and carries out defat, petroleum ether is recovered by filtration, by black Chinese holly
Qi dried bean noodles is dry, by weight adding 61% ethanol solution reflux, extract, with the solid-to-liquid ratio of 1:19,
Extracting temperature 47 DEG C, for the first time backflow 2h, second time and third time backflow 1h, sucking filtration, close
And filtrate, it is cooled to room temperature, stands 24h, centrifugal rejection filter, obtain clear filtrate, with 1BV/h's
Flow velocity joins in processed AB-8 macroporous resin column, is washed to effluent clear and bright, uses
6BV 60% ethanol, with the flow velocity eluting of 1BV/h, is collected eluent, is evaporated to thick paste,
Lyophilization, it is thus achieved that Lycium ruthenicum Murr. procyanidin powder finished product.
(2) allotment: above-mentioned steps is obtained Lycium ruthenicum Murr. procyanidin powder finished product and mixes with pure water,
Adding aspartame, xylitol by weight, mixing, ultrasonic dissolution is placed in 4 DEG C of refrigerators
12-24h, 4000 leave heart 5-10min takes supernatant, adds 0.03-0.05% potassium sorbate,
Mix homogeneously.
(3) fill: take step (2) gained mixed solution and pour into the west of cleaned 10mL
In woods bottle, then cover.
(4) sterilization: use high-temperature instantaneous sterilization, solution is heated at 135-140 DEG C 3-5s.
(5) cooling: the product sterilized is cooled down at 0-25 DEG C.
(6) product examine: subjective visual is brownish red, is visible by naked eyes exogenous impurity, has black Chinese holly
Qi procyanidin faint scent, free from extraneous odour, stored product at 0-25 DEG C.
By implementing technical scheme, following beneficial effect can be reached:
(1) the Lycium ruthenicum Murr. procyanidin oral liquid that the present invention produces, nutritious, clothes
With convenient, old children is all suitable, is suitable for industrialized production processing.
(2) the Lycium ruthenicum Murr. procyanidin oral liquid that the present invention provides, by the black Chinese holly of orthogonal optimization
Qi procyanidin oral liquid prescription, response phase method optimizes Lycium ruthenicum Murr. procyanidin and extracts test, black
Fructus Lycii procyanidin oral liquid subjective appreciation, according to scientific design and optimization technological experiment, preparation
Lycium ruthenicum Murr. procyanidin oral liquid there is good non-oxidizability, absorb very fast, easily preserve,
Rich in nutritive value, is confirmed by functional trial simultaneously, the Lycium ruthenicum Murr. procyanidin mouth of preparation
Take liquid and D-galactose normal saline subcutaneous injection is prepared the anti-ageing of subacute aging mouse model
Old effect, and it is obviously improved SOD vigor in exhausted mining areas serum, promote aging model
In mice serum, GSH-PX vigor and GAT vigor, reduce MDA content, has substantially
Health care, there is wide applicability.
Accompanying drawing explanation
Fig. 1 is shown as concentration of alcohol and solid-liquid ratio reciprocal action and extracts Lycium ruthenicum Murr. procyanidin
The response surface curve chart of rate.
Fig. 2 is shown as concentration of alcohol and solid-liquid ratio reciprocal action and extracts Lycium ruthenicum Murr. procyanidin
Rate affect contour map.
Fig. 3 is shown as concentration of alcohol and Lycium ruthenicum Murr. procyanidin is carried by Extracting temperature reciprocal action
Take the response surface curve chart of rate.
Fig. 4 is shown as concentration of alcohol and Lycium ruthenicum Murr. procyanidin is carried by Extracting temperature reciprocal action
Take rate affects contour map.
Fig. 5 is shown as Extracting temperature and solid-liquid ratio reciprocal action carries to Lycium ruthenicum Murr. procyanidin
Take the response surface curve chart of rate.
Fig. 6 is shown as Extracting temperature and solid-liquid ratio reciprocal action carries to Lycium ruthenicum Murr. procyanidin
Take rate affects contour map.
Detailed description of the invention
Below, for embodiment, the present invention is described, but, the present invention is not limited to following enforcement
Example.
In the present invention, material has: Lycium ruthenicum Murr. powder, xylitol, aspartame and preservative are purchased from
Common raw materials in market；Use petroleum ether, 61% ethanol solution, AB-8 macroporous resin,
D-galactose normal saline is all the common reagent in this area and material.
All material, reagent and the instrument selected in the present invention are all well known in the art, but not
Limiting the enforcement of the present invention, other reagent more well known in the art and equipment are applied both to this
The enforcement of invention implementation below.
Embodiment one: Lycium ruthenicum Murr. procyanidin oral liquid
In percentage by weight, Lycium ruthenicum Murr. procyanidin oral liquid contains Lycium ruthenicum Murr. procyanidin
2-5%, xylitol 5-10%, aspartame 0.03-0.05%, preservative 0.03-0.05%,
Pure water 85-90%.
The preservative that the present invention uses is preferably potassium sorbate, Heat stability is good, and low toxicity can quilt
Digestion decomposes absorption, noresidue.
Embodiment two: the preparation of Lycium ruthenicum Murr. procyanidin oral liquid
Use Lycium ruthenicum Murr. in 50 DEG C of drying, pulverizing, add 8 times amount petroleum ether immersion 24h and enter
Row defat, is recovered by filtration petroleum ether, by dry for Lycium ruthenicum Murr. dried bean noodles, by weight consolidating with 1:19
Liquor ratio adds 61% ethanol solution reflux, extract, and Extracting temperature 47 DEG C, through refluxing several times, taking out
Filter, merging filtrate, it is cooled to room temperature, stands 24h, centrifugal rejection filter, obtain clear filtrate, with
The flow velocity of 1BV/h joins in processed AB-8 macroporous resin column, is washed to effluent
Clear and bright, with 6BV 60% ethanol with the flow velocity eluting of 1BV/h, collect eluent, concentrating under reduced pressure
To thick paste, lyophilization, it is thus achieved that Lycium ruthenicum Murr. procyanidin powder finished product, by former for the Lycium ruthenicum Murr. of preparation
Anthocyanidin powder finished product 2g mixes with pure water 85g, by weight adding aspartame 0.03g, wood
Sugar alcohol 5g, mixing, ultrasonic dissolution is placed on 12-24h in 4 DEG C of refrigerators, and 4000 leave the heart
5-10min, takes supernatant, adds 0.03g potassium sorbate, mix homogeneously, gained mixed solution
Pour in the cillin bottle of cleaned 10mL, then cover, then use high-temperature instantaneous sterilization, will
Solution heats 3-5s at 135-140 DEG C, is cooled down by the product sterilized at 0-25 DEG C,
Subjective visual is brownish red, is visible by naked eyes exogenous impurity, has Lycium ruthenicum Murr. procyanidin delicate fragrance
Abnormal smells from the patient, free from extraneous odour, stored product at 0-25 DEG C.
Embodiment three: the preparation of Lycium ruthenicum Murr. procyanidin oral liquid
Use Lycium ruthenicum Murr. in 50 DEG C of drying, pulverizing, add 8 times amount petroleum ether immersion 24h and enter
Row defat, is recovered by filtration petroleum ether, by dry for Lycium ruthenicum Murr. dried bean noodles, by weight consolidating with 1:19
Liquor ratio adds 61% ethanol solution reflux, extract, and Extracting temperature 47 DEG C, through refluxing several times, taking out
Filter, merging filtrate, it is cooled to room temperature, stands 24h, centrifugal rejection filter, obtain clear filtrate, with
The flow velocity of 1BV/h joins in processed AB-8 macroporous resin column, is washed to effluent
Clear and bright, with 6BV 60% ethanol with the flow velocity eluting of 1BV/h, collect eluent, concentrating under reduced pressure
To thick paste, lyophilization, it is thus achieved that Lycium ruthenicum Murr. procyanidin powder finished product, by former for the Lycium ruthenicum Murr. of preparation
Anthocyanidin powder finished product 5g mixes with pure water 90g, by weight adding aspartame 0.05g, wood
Sugar alcohol 10g, mixing, ultrasonic dissolution is placed on 12-24h in 4 DEG C of refrigerators, and 4000 leave the heart
5-10min, takes supernatant, adds 0.05g potassium sorbate, mix homogeneously, gained mixed solution
Pour in the cillin bottle of cleaned 10mL, then cover, then use high-temperature instantaneous sterilization, will
Solution heats 3-5s at 135-140 DEG C, is cooled down by the product sterilized at 0-25 DEG C,
Subjective visual is brownish red, is visible by naked eyes exogenous impurity, has Lycium ruthenicum Murr. procyanidin delicate fragrance
Abnormal smells from the patient, free from extraneous odour, stored product at 0-25 DEG C.
Embodiment four: the preparation of Lycium ruthenicum Murr. procyanidin oral liquid
Use Lycium ruthenicum Murr. in 50 DEG C of drying, pulverizing, add 8 times amount petroleum ether immersion 24h and enter
Row defat, is recovered by filtration petroleum ether, by dry for Lycium ruthenicum Murr. dried bean noodles, by weight consolidating with 1:19
Liquor ratio adds 61% ethanol solution reflux, extract, and Extracting temperature 47 DEG C, through refluxing several times, taking out
Filter, merging filtrate, it is cooled to room temperature, stands 24h, centrifugal rejection filter, obtain clear filtrate, with
The flow velocity of 1BV/h joins in processed AB-8 macroporous resin column, is washed to effluent
Clear and bright, with 6BV 60% ethanol with the flow velocity eluting of 1BV/h, collect eluent, concentrating under reduced pressure
To thick paste, lyophilization, it is thus achieved that Lycium ruthenicum Murr. procyanidin powder finished product, by former for the Lycium ruthenicum Murr. of preparation
Anthocyanidin powder finished product 3g mixes with pure water 86g, by weight adding aspartame 0.05g, wood
Sugar alcohol 6g, mixing, ultrasonic dissolution is placed on 12-24h in 4 DEG C of refrigerators, and 4000 leave the heart
5-10min, takes supernatant, adds 0.04g potassium sorbate, mix homogeneously, gained mixed solution
Pour in the cillin bottle of cleaned 10mL, then cover, then use high-temperature instantaneous sterilization, will
Solution heats 3-5s at 135-140 DEG C, is cooled down by the product sterilized at 0-25 DEG C,
Subjective visual is brownish red, is visible by naked eyes exogenous impurity, has Lycium ruthenicum Murr. procyanidin delicate fragrance
Abnormal smells from the patient, free from extraneous odour, stored product at 0-25 DEG C.
Embodiment five: the preparation of Lycium ruthenicum Murr. procyanidin oral liquid
Use Lycium ruthenicum Murr. in 50 DEG C of drying, pulverizing, add 8 times amount petroleum ether immersion 24h and enter
Row defat, is recovered by filtration petroleum ether, by dry for Lycium ruthenicum Murr. dried bean noodles, by weight consolidating with 1:19
Liquor ratio adds 61% ethanol solution reflux, extract, and Extracting temperature 47 DEG C, through refluxing several times, taking out
Filter, merging filtrate, it is cooled to room temperature, stands 24h, centrifugal rejection filter, obtain clear filtrate, with
The flow velocity of 1BV/h joins in processed AB-8 macroporous resin column, is washed to effluent
Clear and bright, with 6BV 60% ethanol with the flow velocity eluting of 1BV/h, collect eluent, concentrating under reduced pressure
To thick paste, lyophilization, it is thus achieved that Lycium ruthenicum Murr. procyanidin powder finished product, by former for the Lycium ruthenicum Murr. of preparation
Anthocyanidin powder finished product 3.5g mixes with pure water 89g, addition aspartame 0.03g by weight,
Xylitol 9g, mixing, ultrasonic dissolution is placed on 12-24h in 4 DEG C of refrigerators, and 4000 leave the heart
5-10min, takes supernatant, adds 0.04g potassium sorbate, mix homogeneously, gained mixed solution
Pour in the cillin bottle of cleaned 10mL, then cover, then use high-temperature instantaneous sterilization, will
Solution heats 3-5s at 135-140 DEG C, is cooled down by the product sterilized at 0-25 DEG C,
Subjective visual is brownish red, is visible by naked eyes exogenous impurity, has Lycium ruthenicum Murr. procyanidin delicate fragrance
Abnormal smells from the patient, free from extraneous odour, stored product at 0-25 DEG C.
Embodiment six: the preparation of Lycium ruthenicum Murr. procyanidin oral liquid
Use Lycium ruthenicum Murr. in 50 DEG C of drying, pulverizing, add 8 times amount petroleum ether immersion 24h and enter
Row defat, is recovered by filtration petroleum ether, by dry for Lycium ruthenicum Murr. dried bean noodles, by weight consolidating with 1:19
Liquor ratio adds 61% ethanol solution reflux, extract, and Extracting temperature 47 DEG C, through refluxing several times, taking out
Filter, merging filtrate, it is cooled to room temperature, stands 24h, centrifugal rejection filter, obtain clear filtrate, with
The flow velocity of 1BV/h joins in processed AB-8 macroporous resin column, is washed to effluent
Clear and bright, with 6BV 60% ethanol with the flow velocity eluting of 1BV/h, collect eluent, concentrating under reduced pressure
To thick paste, lyophilization, it is thus achieved that Lycium ruthenicum Murr. procyanidin powder finished product, by former for the Lycium ruthenicum Murr. of preparation
Anthocyanidin powder finished product 4g mixes with pure water 87g, by weight adding aspartame 0.04g, wood
Sugar alcohol 7g, mixing, ultrasonic dissolution is placed on 12-24h in 4 DEG C of refrigerators, and 4000 leave the heart
5-10min, takes supernatant, adds 0.03g potassium sorbate, mix homogeneously, gained mixed solution
Pour in the cillin bottle of cleaned 10mL, then cover, then use high-temperature instantaneous sterilization, will
Solution heats 3-5s at 135-140 DEG C, is cooled down by the product sterilized at 0-25 DEG C,
Subjective visual is brownish red, is visible by naked eyes exogenous impurity, has Lycium ruthenicum Murr. procyanidin delicate fragrance
Abnormal smells from the patient, free from extraneous odour, stored product at 0-25 DEG C.
Embodiment seven: the preparation of Lycium ruthenicum Murr. procyanidin oral liquid
The present invention specifically provides the preparation method of embodiment one Lycium ruthenicum Murr. procyanidin oral liquid, tool
Body uses techniques below step to prepare:
(1) preparation of Lycium ruthenicum Murr. procyanidin: by Lycium ruthenicum Murr. 50 DEG C of drying, pulverized 60
Mesh sieve, adds 8 times amount petroleum ether immersion 24h and carries out defat, petroleum ether is recovered by filtration, by black Chinese holly
Qi dried bean noodles is dry, by weight adding 61% ethanol solution reflux, extract, with the solid-to-liquid ratio of 1:19,
Extracting temperature 47 DEG C, for the first time backflow 2h, second time and third time backflow 1h, sucking filtration, close
And filtrate, it is cooled to room temperature, stands 24h, centrifugal rejection filter, obtain clear filtrate, with 1BV/h's
Flow velocity joins in processed AB-8 macroporous resin column, is washed to effluent clear and bright, uses
6BV 60% ethanol, with the flow velocity eluting of 1BV/h, is collected eluent, is evaporated to thick paste,
Lyophilization, it is thus achieved that Lycium ruthenicum Murr. procyanidin powder finished product.
(2) allotment: above-mentioned steps is obtained Lycium ruthenicum Murr. procyanidin powder finished product and mixes with pure water,
Adding aspartame, xylitol by weight, mixing, ultrasonic dissolution is placed in 4 DEG C of refrigerators
12-24h, 4000 leave heart 5-10min takes supernatant, adds 0.03-0.05% potassium sorbate,
Mix homogeneously.
(3) fill: take step (2) gained mixed solution and pour into the west of cleaned 10mL
In woods bottle, then cover.
(4) sterilization: use high-temperature instantaneous sterilization, solution is heated at 135-140 DEG C 3-5s.
(5) cooling: the product sterilized is cooled down at 0-25 DEG C.
(6) product examine: subjective visual is brownish red, is visible by naked eyes exogenous impurity, has black Chinese holly
Qi procyanidin faint scent, free from extraneous odour, stored product at 0-25 DEG C.
Embodiment eight: prepared by the optimization of Lycium ruthenicum Murr. procyanidin oral liquid
It is respectively adopted the most different formula as preparation Lycium ruthenicum Murr. procyanidin oral liquid component:
Formula 1: Fructus Lycii procyanidin powder 2g, aspartame 0.03g, xylitol 5g, Pyrusussuriensis
Acid potassium 0.03 and pure water 85g.
Formula 2: Fructus Lycii procyanidin powder 5g, aspartame 0.05g, xylitol 10g, mountain
Potassium sorbate 0.05 and pure water 90g.
Formula 3: Fructus Lycii procyanidin powder 3g, aspartame 0.05g, xylitol 6g, Pyrusussuriensis
Acid potassium 0.04 and pure water 86g.
Formula 4: Fructus Lycii procyanidin powder 3.5g, aspartame 0.03g, xylitol 9g, mountain
Potassium sorbate 0.04 and pure water 90g.
Formula 5: Fructus Lycii procyanidin powder 4g, aspartame 0.04g, xylitol 9g, Pyrusussuriensis
Acid potassium 0.03 and pure water 87g.
Five different formulations of above-mentioned offer are prepared Lycium ruthenicum Murr. according to following technique respectively former
Anthocyanin oral solution:
The Lycium ruthenicum Murr. procyanidin powder finished product of preparation is mixed with pure water, adds A Si by weight
Ba Tian, xylitol, mixing, ultrasonic dissolution is placed on 12-24h in 4 DEG C of refrigerators, 4000 turns
Centrifugal 5-10min, takes supernatant, adds potassium sorbate, mix homogeneously, gained mixed solution
Pour in the cillin bottle of cleaned 10mL, then cover, then use high-temperature instantaneous sterilization, will
Solution heats 3-5s at 135-140 DEG C, is cooled down by the product sterilized at 0-25 DEG C,
Subjective visual is brownish red, is visible by naked eyes exogenous impurity, has Lycium ruthenicum Murr. procyanidin delicate fragrance
Abnormal smells from the patient, free from extraneous odour, stored product at 0-25 DEG C.
1. response phase method optimizes the extraction test of Lycium ruthenicum Murr. procyanidin, and experimental factor is shown in Table with level
1:
Table 1: response surface experiments factor and water-glass
Response surface experiments design and result:
Design of experiment analysis is carried out, its knot according to Box-Benhnken center combination design principle
Fruit is shown in Table 2.The method of the polynomial fitting reality to table 2 is used by Design Expert8.0.6
Test data to be fitted, obtain Lycium ruthenicum Murr. extraction ratio Y to concentration of alcohol (A), solid-liquid ratio (B),
The multinomial regression model of secondary of extraction temperature (C) is:
Y=10.31+0.20A-0.12B-0.29C-0.26AB+0.16AC+0.30BC-0.48A2-0.4
8B2-0.29C2
In response surface analysis, each factor reciprocal action is referring specifically to accompanying drawing 1 to accompanying drawing 6.
Table 2: response surface experiments design and result
2. orthogonal optimization Lycium ruthenicum Murr. procyanidin oral liquid
Design experiment of single factor, probes into Lycium ruthenicum Murr. procyanidin powder, xylitol, sorbic acid respectively
Potassium and the impact on Lycium ruthenicum Murr. procyanidin oral liquid sense organ of the aspartame Different adding amount.At list
Four factor three horizontal quadrature experiments, orthogonal test factor and level is carried out on the basis of Factor Experiment
It is shown in Table 3.
Table 3: orthogonal test factor and level
3, Lycium ruthenicum Murr. procyanidin oral liquid subjective appreciation
Above-mentioned experiment basis carries out sense organ to the Lycium ruthenicum Murr. procyanidin oral liquid after allotment
Evaluating, standard is shown in Table 4.
Table 4: Lycium ruthenicum Murr. procyanidin oral liquid subjective appreciation table
Orthogonal optimization Lycium ruthenicum Murr. procyanidin oral liquid result of the test and analysis
Table: 5: orthogonal experiments and analysis
As can be seen from Table 5, in the range of EXPERIMENTAL DESIGN, Lycium ruthenicum Murr. is administered orally by aspartame
Secondly the impact of liquid quality is maximum is Lycium ruthenicum Murr. procyanidin, aspartame and potassium sorbate.
The optimum feed stock of Lycium ruthenicum Murr. oral liquid is joined for A3B1C3D1, i.e. Lycium ruthenicum Murr. procyanidin 2-5%,
Xylitol 5-10%, aspartame 0.03-0.05%, preservative 0.03-0.05%, preferably
Component is Lycium ruthenicum Murr. procyanidin 5%, xylitol 5%, aspartame 0.05%, potassium sorbate
0.03%.
By employing Lycium ruthenicum Murr. procyanidin as pure natural addictive, with Lycium ruthenicum Murr. procyanidin
2-5%, xylitol 5-10%, aspartame 0.03-0.05%, preservative 0.03-0.05%,
Pure water 85-90% is as formula, it is provided that the extraction and preparation technique of Lycium ruthenicum Murr. procyanidin, will
Lycium ruthenicum Murr. powder adds 61% ethanol solution reflux, extract, Extracting temperature with the solid-to-liquid ratio of 1:19
47 DEG C, according to scientific design and optimization technological experiment, the Lycium ruthenicum Murr. procyanidin oral liquid of preparation
In brown color, color and luster is evenly distributed, and has the distinctive fragrance of Lycium ruthenicum Murr., and sense of taste is coordinated soft, clear
Clear bright, without precipitation, and the exogenous impurity being visible by naked eyes, there is Lycium ruthenicum Murr. procyanidin mouth
Take the typicality of liquid idiosyncrasy and product.
Embodiment nine: Lycium ruthenicum Murr. procyanidin oral liquid functional trial
Defying age, slow down aging are everyone dreams.From the perspective of modern nutriology,
Can be with slow down aging containing anti-oxidation active substance by eating.
Select healthy male mice 60, be randomly divided into 5 groups, often group 10, respectively gavage
It is former that the different formulations that above-described embodiment one to embodiment seven of various dose provides prepares Lycium ruthenicum Murr.
Anthocyanin oral solution, Continuous Observation 14d, every day entry group rat ordinary circumstance, toxicity are anti-
Answer and death condition.
Selecting healthy male mice 60, often group ten, is randomly divided into five groups and is respectively as follows: blank
Matched group, model control group, Fructus Lycii procyanidin oral liquid high dose group (0.66/kg.bw.d),
Middle dosage group (0.33/kg.bw.d), low dose group (0.165/kg.bw.d), fill continuously
Stomach 30d；In addition to normal group, remaining is respectively organized and uses D-galactose normal saline subcutaneous injection to cause hero
Property mice subacute aging；It is anesthetized with ether mice after secondary gavage 2h and records male mice serum
Middle SOD, GSH-PX, CAT and MDA content.
Table 6: Lycium ruthenicum Murr. procyanidin oral liquid D-galactose is caused SOD in exhausted mining areas serum,
The impact of GSH-PX, CAT and MDA content
Result: experimental result is shown in Table 6, compares with model group, and Lycium ruthenicum Murr. prepared by the present invention is former
D-galactose normal saline subcutaneous injection is prepared subacute aging mice mould by anthocyanin oral solution
The anti-aging effects of type, and it is obviously improved SOD vigor in exhausted mining areas serum, promote
In exhausted mining areas serum, GSH-PX vigor and GAT vigor, reduce MDA content.
The Lycium ruthenicum Murr. procyanidin oral liquid of the offer that the present invention provides in sum, by orthogonal
Optimizing Lycium ruthenicum Murr. procyanidin oral liquid prescription, response phase method optimizes Lycium ruthenicum Murr. procyanidin and extracts
Test, Lycium ruthenicum Murr. procyanidin oral liquid subjective appreciation, real according to scientific design and optimization technique
Testing, the Lycium ruthenicum Murr. procyanidin oral liquid of preparation has good non-oxidizability, absorbs very fast,
Easily preserve, rich in nutritive value, confirmed, the Lycium ruthenicum Murr. of preparation is former simultaneously by functional trial
D-galactose normal saline subcutaneous injection is prepared subacute aging mice mould by anthocyanin oral solution
The anti-aging effects of type, and it is obviously improved SOD vigor in exhausted mining areas serum, promote
In exhausted mining areas serum, GSH-PX vigor and GAT vigor, reduce MDA content,
There is obvious health care, there is wide applicability.
As it has been described above, the present invention can preferably be realized, the above embodiments are only to this
Bright preferred implementation is described, and is not defined the scope of the present invention, is not taking off
On the premise of the present invention designs spirit, the those of ordinary skill in the art technical side to the present invention
The various changes and improvements that case is made, all should fall in the protection domain that present invention determine that.
Claims (3)
1. a Lycium ruthenicum Murr. procyanidin oral liquid, in percentage by weight, containing Lycium ruthenicum Murr. procyanidin 2-5%, xylitol 5-10%, aspartame 0.03-0.05%, preservative 0.03-0.05%, pure water 85-90%.
Lycium ruthenicum Murr. procyanidin oral liquid the most according to claim 1, it is characterised in that described preservative uses potassium sorbate.
3. the preparation method of a Lycium ruthenicum Murr. procyanidin oral liquid according to claim 1, it is characterised in that concrete employing techniques below step prepares:
(1) preparation of Lycium ruthenicum Murr. procyanidin: by Lycium ruthenicum Murr. 50 DEG C of drying, pulverized 60 mesh sieves, add 8 times amount petroleum ether immersion 24h and carry out defat, petroleum ether is recovered by filtration, by dry for Lycium ruthenicum Murr. dried bean noodles, by weight adding 61% ethanol solution reflux, extract, with the solid-to-liquid ratio of 1:19, Extracting temperature 47 DEG C, backflow 2h for the first time, second time and third time backflow 1h, sucking filtration, merging filtrate, it is cooled to room temperature, stand 24h, centrifugal rejection filter, obtain clear filtrate, join in processed AB-8 macroporous resin column with the flow velocity of 1BV/h, it is washed to effluent clear and bright, with 6BV 60% ethanol with the flow velocity eluting of 1BV/h, collect eluent, it is evaporated to thick paste, lyophilization, obtain Lycium ruthenicum Murr. procyanidin powder finished product；
(2) allotment: above-mentioned steps is obtained Lycium ruthenicum Murr. procyanidin powder finished product and mixes with pure water, add aspartame, xylitol by weight, mixing, ultrasonic dissolution is placed on 12-24h in 4 DEG C of refrigerators, 4000 leave heart 5-10min takes supernatant, adds 0.03-0.05% potassium sorbate, mix homogeneously；
(3) fill: take in the cillin bottle that step (2) gained mixed solution pours into cleaned 10mL, then cover；
(4) sterilization: use high-temperature instantaneous sterilization, solution is heated at 135-140 DEG C 3-5s；
(5) cooling: the product sterilized is cooled down at 0-25 DEG C；
(6) product examine: subjective visual is brownish red, is visible by naked eyes exogenous impurity, has Lycium ruthenicum Murr. procyanidin faint scent, free from extraneous odour, stored product at 0-25 DEG C.
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