CN105903089A - Application of gelatin/polycaprolactone nanofiber material in surgical postoperative adhesion prevention - Google Patents
Application of gelatin/polycaprolactone nanofiber material in surgical postoperative adhesion prevention Download PDFInfo
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- CN105903089A CN105903089A CN201610296574.7A CN201610296574A CN105903089A CN 105903089 A CN105903089 A CN 105903089A CN 201610296574 A CN201610296574 A CN 201610296574A CN 105903089 A CN105903089 A CN 105903089A
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/04—Macromolecular materials
- A61L31/041—Mixtures of macromolecular compounds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/14—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L31/148—Materials at least partially resorbable by the body
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- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0015—Electro-spinning characterised by the initial state of the material
- D01D5/003—Electro-spinning characterised by the initial state of the material the material being a polymer solution or dispersion
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
- D01D5/0076—Electro-spinning characterised by the electro-spinning apparatus characterised by the collecting device, e.g. drum, wheel, endless belt, plate or grid
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
- D01D5/0092—Electro-spinning characterised by the electro-spinning apparatus characterised by the electrical field, e.g. combined with a magnetic fields, using biased or alternating fields
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
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- D01F8/00—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof
- D01F8/02—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from cellulose, cellulose derivatives, or proteins
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- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
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- D01F8/00—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof
- D01F8/04—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from synthetic polymers
- D01F8/14—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from synthetic polymers with at least one polyester as constituent
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- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
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Abstract
The invention relates to an application of a gelatin/polycaprolactone nanofiber material. The gelatin/polycaprolactone nanofiber material is prepared through the following methods: dissolving gelatin and polycaprolactone in trifluoroethanol, adding acetic acid into the solution, and carrying out stirring until complete dissolution is achieved so as to obtain a transparent gelatin/polycaprolactone spinning solution; extracting the gelatin/polycaprolactone spinning solution, fixing the gelatin/polycaprolactone spinning solution on an electrostatic spinning device for electrospinning, collecting fibrous membranes, and carrying out vacuum drying, thereby obtaining the gelatin/polycaprolactone nanofiber material. The gelatin/polycaprolactone nanofiber material has the advantages that the pore size is small, so that the contact between wound surfaces and the infiltration of in-vivo inflammatory cells can be effectively prevented; the gelatin/polycaprolactone nanofiber material is flexible, can be used for reducing friction among tissues and is not easy to tear; the gelatin/polycaprolactone nanofiber material is good in biocompatibility and remarkable in adhesion preventing effect. Taking heart postoperative adhesion prevention for example, along with the gradual degradation of the material, autologous body cells can grow inside the material so as to form a cambium with growth activity, and the cambium and surrounding heart sac tissue are merged into one body, so that the adhesion between a heart and a thoracic cavity is effectively prevented.
Description
Technical field
The present invention relates to tissue engineering technique field, specifically, be a kind of gelatin/polycaprolactone Nanowire
Dimension material application in surgery post-operation adhesion preventing.
Background technology
Tissue adhesion is the clinical picture that surgical field is common, such as department of cerebral surgery, abdominal surgery, department of obstetrics and gynecology, the heart
Dirty surgery.If generation adhesion, have may result in serious complication, such as: department of cerebral surgery post-craniotomy dura mater
Defect, cicatricial adhesion can cause postoperative epilepsy；Adhesion after abdominal surgery can cause intestinal obstruction, abdomen basin
Chamber pain；Intrauterine adhesion after obstetrics and gynecology operation, serious may result in be infertile, periodically lower abdominal pain；Heart
The adhesion in postoperative cardiac and thoracic cavity can increase the risk of second operation, the life of serious jeopardized infant.Cause
This, tissue adhesion has been important difficult medical problem urgently to be resolved hurrily since history of operation.Solve adhesion at present the simplest
Way is to use so-called " physical barriers " reliably again, i.e. material is placed in the cutting part of tissue, to fibre
The growth of dimensional tissue takes blocking effect.In early days, use silica gel, gelfoam, hyaluronate sodium both at home and abroad more
Deng as isolated material, prevent the generation of adhesion, but they all affect use because of respective limitation.Silicon
Glue is applied relatively early in anti, but the histocompatibility of silica gel product is poor, it is impossible to be absorbed by organisms, and makees
Mechanical stimulating action can be produced for foreign body longer-term persistence.Gelfoam has certain anti after surgery in early days
Effect, but gelfoam is sucked blood and can be expanded, and forms hematoma, and the later stage can induce and increase the weight of the shape of cicatrix
Become.Hyaluronate sodium has antiinflammatory, pain relieving, the effect of promotion wound healing, but hyaluronate sodium is water-soluble
Property good, bad mechanical property, the most degradable, the most in early days there is anti effect.In recent years,
Sight is concentrated on bioabsorbable membrane by medical expert and materials specialist both at home and abroad, and the material constituting film has
Good biocompatibility and blood compatibility, can be effectively isolated the contact of wound surface after surgery, and complete
Can be taken out without second operation by human body degraded and absorbed after isolation purpose.With other biomedical products class
Together, the absorbent antiseize product that China is current relies primarily on import.The product variety of independent research is less,
Mainly include polylactic acid membrane and chitosan film, all there is pliability not enough, the defects such as biocompatibility is not good enough.
Therefore exploitation a new generation absorbent antiseize film, be Clinical Surgery in the urgent need to.
Gelatin is a kind of native protein, by collagen hydrolysate, and its structure and bio-tissue structure
Similar, therefore there is good biocompatibility.Can degrade voluntarily under the effect of its enzyme, salt etc. in vivo,
Low molecule product can be absorbed by the body, or is excreted by Excretory system, without having side effects,
Have been widely used for food and medicinal industry at present.
Polycaprolactone (polycaprolactone, PCL) be through the one of U.S. FDA certification, approval is medical can
Biodegradable synthetic material, the product after PCL degraded is CO2And H2O, to human non-toxic, has good
Good biocompatibility and excellent mechanical property.Have been widely used for drug controllable release carrier at present, carefully
Born of the same parents and tissue culture's support, Wholly-degradable operation suture thread etc..
Electrostatic spinning technique is that one utilizes electric field force that polymer solution or melt are prepared as fibre diameter to exist
The nano-micrometer superfine fibre technology of preparing of tens nanometers to several microns.Receiving prepared by electrostatic spinning technique-
Micrometer fibers support has high specific surface area, loose structure, can the Nomenclature Composition and Structure of Complexes of bionic extracellular matrix,
Thus provide the ideal microenvironment of a kind of similar natural extracellular matrix with Function for the growth of cell,
Become the focus of current tissue engineering bracket research.
But, gelatin is a kind of natural macromolecular material, containing many cellular binding sites, but biodegradation
Too fast, mechanical performance and electrospinning poor performance.On the contrary, polycaprolactone is a kind of synthesis macromolecular material, degraded
Speed is slow, good mechanical property, easy electrospinning filamentation, but does not has the affine site of cell, in order to reach the two
Having complementary advantages, Chinese patent literature CN102242463A discloses a kind of electro-spinning for gelatin/poly-in oneself
The method of ester composite nano-fiber membrane.Using this nano fibrous membrane as support, be applied to tissue engineering bone/cartilage,
The research of skin, experiment shows: this Biocompatibility is good, using chondrocyte as seed cell, should
Material can construct the three-dimensional cartilaginous tissue of elaborate.Mix altogether with chondrocyte and bone marrow stroma stem cell
Cultivating as seed cell, the cartilaginous tissue of formation is the most very uniform stable.Careful using horn cell as planting
Born of the same parents, novel gelatin/polycaprolactone material is conducive to the reparation of skin injury.But the one about the present invention
Gelatin/polycaprolactone nano-fiber material application in surgery operation antiblocking yet there are no report.
Summary of the invention
It is an object of the invention to, for deficiency of the prior art, develop the material of a kind of new anti, subtract
The misery of hypopathia people, reduces surgical operation sequela, improves surgical quality, it is provided that a kind of gelatin/gather in oneself
The application in surgery post-operation adhesion preventing of the ester nano-fiber material.
For achieving the above object, the present invention adopts the technical scheme that: a kind of gelatin/polycaprolactone Nanowire
Dimension material application in the material preparing surgical postoperative anti, described gelatin/polycaprolactone Nanowire
Dimension material prepares by the following method: gelatin and polycaprolactone is dissolved in trifluoroethanol, and adds in solution
Entering micro-acetic acid, stirring, to being completely dissolved, obtains transparent gelatin/polycaprolactone spinning liquid；Extraction gelatin/
Polycaprolactone spinning liquid, is fixed on electrostatic spinning apparatus and carries out electrospinning, collects fibrous membrane and is vacuum dried
Obtain.
The described antiseized postcardiac surgery that is linked as prevents pericardiosymphysis.
The mass ratio of gelatin and polycaprolactone is 1:0.1～10.Preferably, gelatin and the mass ratio of polycaprolactone
For 30:70～70:30.
The mass ratio of gelatin and polycaprolactone is 1:1.
Acetic acid volume ratio in spinning liquid is 0.1-2%.
Acetic acid volume ratio in spinning liquid is 0.2%.
Described gelatin/polycaprolactone nano-fiber material prepares by the following method: by 0.5g gelatin and 0.5g
Polycaprolactone is dissolved in 10ml trifluoroethanol, and adds 20ul acetic acid in solution, stirs to being completely dissolved,
Obtain transparent gelatin/polycaprolactone spinning liquid；Extraction gelatin/polycaprolactone spinning liquid, is fixed on Static Spinning
Carrying out electrospinning on silk device, electrospinning parameter is: voltage 8-12kv, receiving range 10-15cm, injection rate
1-5ml/h, temperature 18-28 DEG C, relative humidity 40-60%；Use cylinder as receiving device, collect fiber
Film is then placed in vacuum drying oven removing the organic solvent of residual and get final product.
The invention has the advantages that:
1, the gelatin prepared by the present invention/polycaprolactone nanofiber membrane aperture is little, can effectively intercept wound surface
Contact, the infiltration of inflammatory cell in body.
2, the gelatin prepared by the present invention/polycaprolactone nano fibrous membrane is pliable and tough, can reduce rubbing between tissue
Wiping is also less prone to tear.
3, gelatin/polycaprolactone nanometer fiber membrane biological compatibility is good, and preventing adhesiving effect is notable.With heart art
As a example by rear anti, along with the gradually degraded of this material, body own cells can be grown into material internal, shape
Become there is the cambium of growth activity, and this cambium combines together with surrounding pericardial tissue, effectively
Intercept the adhesion of heart and thoracic cavity.
Accompanying drawing explanation
Accompanying drawing 1: gelatin/polycaprolactone nano fibrous membrane substantially sees (2 × 2cm).
Accompanying drawing 2: gelatin/polycaprolactone nano fibrous membrane stereoscan photograph (amplifies 1000 times).
Accompanying drawing 3: gelatin/polycaprolactone nanofiber film dynamic performance result.
Accompanying drawing 4: gelatin/polycaprolactone nanometer fiber membrane biological compatibility result (amplifies 200 times).
Accompanying drawing 5: the foundation of rabbit hearts post-operation adhesion preventing model.
Accompanying drawing 6: gelatin/polycaprolactone (30:70) month after operation is substantially seen.
A: material group is substantially seen after opening breast；
B: material group is substantially seen after opening breast.
Accompanying drawing 7: gelatin/polycaprolactone (50:50) month after operation is substantially seen.
A: material group is substantially seen after opening breast；
B: material group is substantially seen after opening breast.
Accompanying drawing 8: gelatin/polycaprolactone (70:30) month after operation is substantially seen.
A: material group is substantially seen after opening breast；
B: material group is substantially seen after opening breast.
Accompanying drawing 9: gelatin/polycaprolactone (50:50) is substantially seen for postoperative 2 months.
A: negative control group is substantially seen after opening breast；
B: positive controls is substantially seen after opening breast；
C: material group is substantially seen after opening breast；
D: material group is substantially seen after opening breast.
Accompanying drawing 10: gelatin/polycaprolactone (50:50) 3 months after operation is substantially seen.
A: negative control group is substantially seen after opening breast；
B: positive controls is substantially seen after opening breast；
C: material group is substantially seen after opening breast；
D: material group is substantially seen after opening breast.
Accompanying drawing 11: gelatin/polycaprolactone (50:50) is substantially seen for postoperative 6 months.
A: negative control group is substantially seen after opening breast；
B: positive controls is substantially seen after opening breast；
C: material group is substantially seen after opening breast；
D: material group is substantially seen after opening breast.
Detailed description of the invention
The detailed description of the invention provided the present invention below in conjunction with the accompanying drawings elaborates.
1 materials and methods
The preparation of 1.1 gelatin/polycaprolactone electro spinning nanometer fiber membrane
1.1.1 the preparation of gelatin/polycaprolactone (30:70) electro spinning nanometer fiber membrane
0.3g gelatin and 0.7g polycaprolactone are dissolved in 10ml trifluoroethanol, and in solution, add 10ul
Acetic acid, 24h is to being completely dissolved in stirring, obtains transparent gelatin/polycaprolactone spinning liquid.Select 10ml's
Syringe, the syringe needle of 1.2mm internal diameter, extracts gelatin/polycaprolactone spinning liquid, is fixed on electrostatic spinning dress
Putting and carry out electrospinning, parameter is: voltage 8-12kv, receiving range 10-15cm, injection rate 2.5ml/h,
Temperature 18-28 DEG C, relative humidity 40-60%.Use cylinder as receiving device, spinning 0.75h, obtain
Gelatin/polycaprolactone nano fibrous membrane, puts into that to remove the organic solvent of residual in vacuum drying oven standby.
1.1.2 the preparation of gelatin/polycaprolactone (50:50) electro spinning nanometer fiber membrane
0.5g gelatin and 0.5g polycaprolactone are dissolved in 10ml trifluoroethanol, and in solution, add 20ul
Acetic acid, 24h is to being completely dissolved in stirring, obtains transparent gelatin/polycaprolactone spinning liquid.Select 10ml's
Syringe, the syringe needle of 1.2mm internal diameter, extracts gelatin/polycaprolactone spinning liquid, is fixed on electrostatic spinning dress
Putting and carry out electrospinning, parameter is: voltage 8-12kv, receiving range 10-15cm, injection rate 2ml/h,
Temperature 18-28 DEG C, relative humidity 40-60%.Use cylinder as receiving device, spinning 1h, obtain bright
Glue/polycaprolactone nano fibrous membrane, puts into that to remove the organic solvent of residual in vacuum drying oven standby.
1.1.3 the preparation of gelatin/polycaprolactone (70:30) electro spinning nanometer fiber membrane
0.7g gelatin and 0.3g polycaprolactone are dissolved in 10ml trifluoroethanol, and in solution, add 30ul
Acetic acid, 24h is to being completely dissolved in stirring, obtains transparent gelatin/polycaprolactone spinning liquid.Select 10ml's
Syringe, the syringe needle of 1.2mm internal diameter, extracts gelatin/polycaprolactone spinning liquid, is fixed on electrostatic spinning dress
Putting and carry out electrospinning, parameter is: voltage 8-12kv, receiving range 10-15cm, injection rate 1.5ml/h,
Temperature 18-28 DEG C, relative humidity 40-60%.Use cylinder as receiving device, spinning 1.5h, obtain bright
Glue/polycaprolactone nano fibrous membrane, puts into that to remove the organic solvent of residual in vacuum drying oven standby.
The qualification of 1.2 gelatin/polycaprolactone electro spinning nanometer fiber membrane
1.2.1 the observation of nano fibrous membrane pattern
Dried nano fibrous membrane is cut into small pieces, is attached on object stage gold-plated 90 seconds, adding at 10KV
Under speed voltage, observe the form of electrostatic spinning nano fiber by scanning electron microscope (JSM-56OOLV) and clap
According to.Based on gained SEM image, use the Static Spinning analyzing software I mageJ (NIH, USA) to preparation
The diameter of silk nanofiber measures.
1.2.2 the detection of nanofiber film dynamic performance
Gelatin/polycaprolactone electro spinning nanometer fiber membrane is cut into rectangle, long 5cm, wide 1cm, and thickness is about
0.2-0.3mm, puts into obtained sample in PBS and soaks 2 hours, be then fixed on material testing machine
(Instron-3343；USA), on, with 10mm/min draw speed, sample is carried out extension test.
1.2.3 nanometer fiber membrane biological compatibility test
Fibroblast is inoculated on the timbering material disinfected, applies the high sugar of common 10%FBS
DMEM culture fluid is cultivated, and after 1 and 7 day, utilizes SEM and fluorescence microscope cell propping up
Growing state on frame.
The foundation of 1.3 rabbit hearts post-operation adhesion preventing models.
Choose the new zealand white rabbit of 2-3kg as animal pattern, pentobarbital (consumption 30mg/kg) fiber crops
Liquor-saturated, to anaesthetize laggard circulation of qi promoting cannula and access respirator, ventilation is 25ml/ time, 36 times/min.Breastbone
Center incision of skin, opens breast with staight scissors at median sternotomy, and struts with Sternal distraction device.Negative control group:
It is left intact and directly closes breast.Positive controls: separate and organize at the bottom of thymus and the heart, identify pericardium, the right heart
Outdoor pericardium stringer cuts off about 2 × 2cm, with cotton swab friction heart surface to petechial hemorrhage, closes breast.Real
Test group: after cotton swab friction heart surface to petechial hemorrhage, nano fibrous membrane is cut into 2 × 2cm square
(as shown in Figure 1), sterilization is placed at defect, does the fixing of four angles with 6-0proline suture and closes breast.
1.4 preventing adhesiving effect evaluations
The art later moon, rabbit overdose of anesthesia is sacrificed by pentobarbital (consumption 60mg/kg), hits exactly and opens breast,
And strut with Sternal distraction device, separate breastbone and pericardium, observe pericardium whether with heart surface adhesion, and use
High definition camera is taken pictures.
2 results
The preparation of 2.1 gelatin/polycaprolactone electro spinning nanometer fiber membrane
Fig. 1 is the substantially sight by the nano fibrous membrane prepared by electrostatic spinning technique, from generally, and material
Expecting white, quality is soft, and the random cutting of big I, thickness can arbitrarily control.
The qualification of 2.2 gelatin/polycaprolactone electro spinning nanometer fiber membrane
2.2.1 the observation of nano fibrous membrane pattern
Fig. 2 is the stereoscan photograph of gelatin/polycaprolactone nano fibrous membrane, the most not
The material of homogenous quantities ratio all can electrospinning wire vent, the fiber of formation is homogeneous, continuous, smooth.Fiber aperture is little,
So not only improve the transport of nutrient substance, can effectively intercept again the contact of wound surface, inflammatory cell in body
Infiltration.
2.2.2 the detection of nanofiber film dynamic performance
Fig. 3 is the mechanical experimental results under prepared gelatin/polycaprolactone nano fibrous membrane hygrometric state.
Figure A is the load-deformation curve of the material of respective different proportion, and figure B is the ultimate tensile strength of material,
Figure C is the maximum tension length of material.It can be seen that gradually rising along with gelatine content, material
The hot strength of material significantly reduces, 4.4 ± 0.43MPa (30:70) drop to 3.2 ± 0.28MPa (50:50),
Finally it is down to 1.1 ± 0.07MPa (70:30).But, the maximum tension length of material but occurs in that by a small margin
Rising, strain be raised to 2.1 ± 0.18 (50:50) by 1.75 ± 0.30 (30:70), be eventually raised to 2.4 ± 0.39
(70:30)。
2.2.3 nanometer fiber membrane biological compatibility test
Fig. 4 is gelatin/polycaprolactone nanometer fiber membrane biological compatibility test result, it can be seen that
Gelatin/polycaprolactone nanometer fiber membrane biological compatibility is good, and cell is well-grown on material, does not occur
Poison epiphenomenon, cell seeding, after material upper 1 day, has had substantial amounts of cell attachment raw on material surface
Long, along with the prolongation of incubation time, cell constantly rises in value, and secretes substantial amounts of extracellular matrix, contacts with each other
And merge, almost cover whole material surface when 7 days.
The foundation of 2.3 rabbit hearts post-operation adhesion preventing models
After Fig. 5 A is for opening breast by rabbit center, removes part pericardial tissue, expose diameter about 15mm
Breach；Figure B is to petechial hemorrhage by the heart surface cotton swab friction under breach in figure A, then uses
The stitching that each group material of size shown in Fig. 1 does four angles is fixed, and sets up anti model.
2.4 post-operation adhesion preventing effect assessments
All in all, negative control group is not owing to destroying pericardium, and after opening breast, heart surface is smooth, it is easy to
Opening with other separate tissue, without any adhesion between heart and pericardium, each tissue is high-visible.Positive control
Directly closing breast, heart surface and pericardium after group pericardium breakage, breastbone adhesion is serious, along with prolonging of Post surgery duration
Long, it is difficult to open each separate tissue, this will extend the time of second operation and increase the difficulty of second operation,
The danger of heart massive hemorrhage sometimes can occur.
For material group, when gelatin/polycaprolactone ratio is 30:70, the mechanical property of material is good,
Operation technique is convenient, but owing to polycaprolactone content is too high, material hydrophilic is poor, and hardness is high, with pericardium
The friction great efforts on surface.The art later moon, material was observed, and discovery adhesion phenomenon is serious, and material is tightly pasted onto
Heart surface, it is impossible to segmentation (Fig. 6).When gelatin/polycaprolactone ratio is 50:50, material hydrophilic
Good, soft smooth, the mechanics of material has slight decline, but entirely appropriate operation stitching operates.Postoperative
Within one month, drawing materials observation, find that the edge of only material and heart surface have slight adhesion, operating theater instruments is very
Can be easily separated, after separation, material is white, effectively intercepts the adhesion (Fig. 7) of heart and thoracic cavity.When gelatin/
When polycaprolactone ratio is 70:30, owing to components of gelatin content is high, prepared nano fibrous membrane mechanical property
Can be decreased obviously, during operation, material easily worn out by needlework, is not suitable for surgical stapling operation.Operation is sewed up reluctantly
After, observation of drawing materials for month, find that adhesion phenomenon is serious, and material is degraded the most substantially, it is impossible to hinder completely
The only generation of adhesion.(Fig. 8).
Preferably, the gelatin that the present invention selects ratio to be 50:50/polycaprolactone nano fibrous membrane is made further
Observing, from Fig. 9, Figure 10, Figure 11 are it can be seen that along with the prolongation of Post surgery duration, gelatin/polycaprolactone
Nano fibrous membrane is the most gradually degraded, and body own cells can be grown into material internal, is formed and has growth activity
Cambium, and this tissue combines together with surrounding pericardial tissue, effectively intercepts the viscous of heart and thoracic cavity
Even.
The above is only the preferred embodiment of the present invention, it is noted that common for the art
Technical staff, on the premise of without departing from the inventive method, it is also possible to makes some improvement and supplements, these
Improve and supplement and also should be regarded as protection scope of the present invention.
Claims (7)
1. a gelatin/polycaprolactone nano-fiber material application in the material of preparation anti, it is special
Levying and be, described gelatin/polycaprolactone nano-fiber material prepares by the following method: by gelatin with poly-
Caprolactone is dissolved in trifluoroethanol, and adds acetic acid in solution, and stirring, to being completely dissolved, obtains transparent
Gelatin/polycaprolactone spinning liquid；Extraction gelatin/polycaprolactone spinning liquid, is fixed on electrostatic spinning apparatus enterprising
Row electrospinning, collects fibrous membrane and carries out being vacuum dried and get final product.
Application the most according to claim 1, it is characterised in that the described antiseized postcardiac surgery that is linked as is prevented
Only pericardiosymphysis.
Application the most according to claim 1, it is characterised in that the mass ratio of gelatin and polycaprolactone is
1:0.1～10.
Application the most according to claim 3, it is characterised in that the mass ratio of gelatin and polycaprolactone is
1:1。
Application the most according to claim 1, it is characterised in that acetic acid volume ratio in spinning liquid is
0.1-2%.
Application the most according to claim 5, it is characterised in that acetic acid volume ratio in spinning liquid is
0.2%.
Application the most according to claim 1, it is characterised in that described gelatin/polycaprolactone nanometer
Fibrous material prepares by the following method: 0.5g gelatin and 0.5g polycaprolactone are dissolved in 10ml trifluoro second
In alcohol, and adding 20ul acetic acid in solution, stirring, to being completely dissolved, obtains transparent gelatin/poly-in oneself
Ester spinning liquid；Extraction gelatin/polycaprolactone spinning liquid, is fixed on electrostatic spinning apparatus and carries out electrospinning, electricity
Spinning parameter is: voltage 8-12kv, receiving range 10-15cm, injection rate 1-5ml/h, temperature 18-28 DEG C,
Relative humidity 40-60%；Use cylinder as receiving device, collect fibrous membrane and be then placed in vacuum drying oven
Middle remove residual organic solvent and get final product.
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| CN114541039A (en) * | 2020-11-26 | 2022-05-27 | 季华实验室 | Preparation method of composite structure porous membrane and composite structure porous membrane prepared by same |
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| CN115944780A (en) * | 2022-12-22 | 2023-04-11 | 上海交通大学医学院附属第九人民医院 | Allogeneic cartilage tissue compound based on immune isolation strategy, preparation method and application thereof |
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Application publication date: 20160831 |