CN1058989A - Process for refractory alcohol active dried yeast - Google Patents
Process for refractory alcohol active dried yeast Download PDFInfo
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- CN1058989A CN1058989A CN 91108644 CN91108644A CN1058989A CN 1058989 A CN1058989 A CN 1058989A CN 91108644 CN91108644 CN 91108644 CN 91108644 A CN91108644 A CN 91108644A CN 1058989 A CN1058989 A CN 1058989A
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Abstract
The present invention relates to a kind of process for refractory alcohol active dried yeast.It is that to adopt a kind of high-temperature resistant strain be that medium slant is cultivated with the wort agar in test tube, is that substratum is cultivated in F bottle graft kind with the wort, with wort and molasses be substratum in the Carlsberg's flask inoculation culture, be that main raw material is added with (NH with molasses
4)
2SO
4, phosphorus source and trace element be substratum in the seeding tank inoculation culture, with molasses be the main (NH that is added with then
4)
2SO
4, the phosphorus source and the trace element substratum in carry out bulk fermentation, after processes such as separation, dryings.Temperature Tolerant Active Dry Yeast with this explained hereafter can normally fermentation under 25-40 ℃ of condition.
Description
The present invention relates to the production technique of a kind of microorganism, particularly a kind of refractory alcohol active dryness zymic production technique.
Can only could normally carry out under 25 to 30 ℃ normal temperature with the yeast wine brewing at present, therefore high brewery of temperature in summer and grain distillery can suffer a loss or have to stop production; Though the high-temperature resistant strain that brewery or grain distillery use has resistant to elevated temperatures characteristic, must could use through 5 to 6 grades of expansions, and bacterial classification is preserved complexity, easily degeneration, easily microbiological contamination in the production.
The production technique that the purpose of this invention is to provide a kind of Temperature Tolerant Active Dry Yeast, the Temperature Tolerant Active Dry Yeast that uses this method to produce can all can normally ferment under 25～40 ℃ condition, and easily preservation, easy to use.
The object of the present invention is achieved like this:
Main processes comprises: technologies such as test tube slant cultivation, the cultivation of F bottle graft kind, Carlsberg's flask inoculation culture, seeding tank fermentation, the fermentation of commodity jar, separating, washing, filter press, granulation, fluidised bed drying, finished product packing.At first screen a kind of high-temperature resistant strain, place the test tube inclined-plane that fills wort agar to cultivate 48 hours for 30 ℃ it; Be that substratum is inoculated 30 ℃ of cultivations 48 hours again with the wort in the F bottle; Be that substratum was cultivated 48 hours for 30 ℃ in the Carlsberg's flask inoculation with wort and molasses mixed solution again; Be substratum inoculation culture 18 hours in the pure culture jar again with molasses; Be main raw material again with molasses, add (NH
4)
2SO
4, phosphorus source and trace element be substratum inoculation culture 30 hours in seeding tank, culture temperature is 30-31 ℃, air quantity is 1 gram O
2About/l gram yeast minute, sugar degree is/100 milliliters of 0.2 grams, and final yeast concn is 160 grams per liters; Be main raw material with molasses again, be added with (NH
4)
2SO
4, phosphorus source and trace element are that substratum inoculation culture in the commodity jar was fermented about 14 hours, and leavening temperature is 30-36 ℃, and pH value 4.8-5.8, air quantity are 1 gram O
2/ l gram yeast minute, yeast concn is 170 grams per liters; With the fermented liquid separating, washing of commodity jar, use the plate-and-frame filter press press filtration again, the yeast that filters gained carries out granulation, carries out low temperature rapid drying with fluidized-bed, and the material top temperature is no more than 35 ℃, and the dry yeast water content is controlled at 5-8%, vacuumizes packing at last.
Adopt production Temperature Tolerant Active Dry Yeast technology provided by the invention can replace whole operations of traditional wine yeast culture fully, use this high temperature alcohol active dried yeast wine brewing, shortened fermentation period, improve liquor ratio of raw material 1%-10%, 25-40 ℃ of fermentation optimal temperature solved the difficult contradiction of high temperature season wine brewing.
Embodiment one:
Filter out a kind of high-temperature resistant strain, it is placed the test tube slant culture 48 hours that fills wort agar, 30 ℃ of culture temperature; Inoculation culture in the F bottle again, with 100-140 gram wort/liter the mashing water culture medium inoculated cultivated 48 hours for 30 ℃; Inoculation culture in Carlsberg's flask again, with 50 gram worts/liter mashing water and liquid molasses be that substratum was cultivated 48 hours for 30 ℃; In the pure culture jar, cultivate again, with 100 gram molasses/liter liquid molasses inoculation culture 18 hours; In the seeding tank fermentation, substratum is to be the main (NH that is added with molasses again
4)
2SO
4, phosphorus source and trace element, main component content is: molasses are 5 times of dry yeast weight, and nitrogen is 7% of dry yeast weight, and Vanadium Pentoxide in FLAKES is 2.5% of a dry yeast weight, and the inoculation culture time is 30 hours, and culture temperature is 30-31 ℃, air quantity is 1 gram O
2/ l gram yeast minute, final yeast concn is 160 grams per liters; Again in commodity jar fermentation, substratum is to be that main additive has (NH with molasses
4)
2SO
4, phosphorus source and trace element, main component is: molasses are 4.5 times of dry yeast weight, and nitrogen is 6.8% of dry yeast weight, Vanadium Pentoxide in FLAKES is 2.2% of a dry yeast weight, and the inoculation fermentation time is 14 hours, and leavening temperature is 30-36 ℃, pH value is 4.8-5.8, and air quantity is 1 gram O
2/ l gram yeast minute; The Yeast proliferation multiple is 6-7 times, final yeast concn is that 170 grams per liters carry out separating, washing with the fermented liquid of commodity jar again, use the plate-and-frame filter press press filtration, the yeast that filters gained carries out granulation, carry out low temperature rapid drying with fluidized-bed, the material top temperature is no more than 35 ℃, and the active dry yeast water content is controlled at 5-8%, vacuumizes packing at last.
Claims (2)
1, a kind of process for refractory alcohol active dried yeast, it comprises the operations such as test tube slant cultivation, the cultivation of F bottle graft kind, Carlsberg's flask inoculation culture, seeding tank fermentation, the fermentation of commodity jar, separating, washing, filter press, granulation, fluidised bed drying, finished product packing of bacterial strain, it is characterized in that: it is to be that medium slant was cultivated 48 hours for 30 ℃ with the wort agar that the test tube slant of bacterial strain is cultivated; It is to be that culture medium inoculated was cultivated 48 hours for 30 ℃ with the wort that F bottle graft kind is cultivated; The Carlsberg's flask inoculation culture is to be that culture medium inoculated is cultivated kinds 18 hours with wort and molasses mixed solution; The seeding tank fermention medium is to be main raw material with molasses, is added with (NH
4)
2SO
4, phosphorus source and trace element, inoculation culture 30 hours, culture temperature are controlled at 30～31 ℃, air quantity is 1 gram O
2/ l restrains yeast, about branch is planted; Commodity jar fermention medium is to be main raw material with molasses, is added with (NH
4)
2SO
4, phosphorus source and trace element, inoculation culture is about 14 hours, the fermentation culture temperature is 30～36 ℃, pH value 4.8-5.8, air quantity are 1 gram O
2/ l restrains yeast, minute; Fluidised bed drying is to adopt low temperature rapid drying, and the material top temperature is no more than 35 ℃.
2,, it is characterized in that the bacterial strain that the test tube slant is cultivated is a kind of high-temperature resistant strain according to claims 1 described process for refractory alcohol active dried yeast.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 91108644 CN1058989A (en) | 1991-08-28 | 1991-08-28 | Process for refractory alcohol active dried yeast |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 91108644 CN1058989A (en) | 1991-08-28 | 1991-08-28 | Process for refractory alcohol active dried yeast |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1058989A true CN1058989A (en) | 1992-02-26 |
Family
ID=4909396
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 91108644 Pending CN1058989A (en) | 1991-08-28 | 1991-08-28 | Process for refractory alcohol active dried yeast |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1058989A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1322109C (en) * | 2002-10-15 | 2007-06-20 | 湖北安琪酵母股份有限公司 | Drying production technique of fluidized bed by starch adsorption of dry oceanic rhodotorula |
| CN101912041A (en) * | 2010-08-02 | 2010-12-15 | 哈尔滨市海澳斯生物科技开发有限公司 | Preparation method of active rhodotorula glutinis powder |
| CN101194011B (en) * | 2005-06-09 | 2012-10-31 | 诺塞斯有限公司 | Dry and/or microencapsulated saccharomyces cerevisiae cells with high content of - (+) -S-adenosyl-L-methionine, process for their preparation and compositions containing them |
| CN104450380A (en) * | 2014-11-25 | 2015-03-25 | 安琪酵母（睢县）有限公司 | Production method for compound yellow rice wine saccharomycopsis |
-
1991
- 1991-08-28 CN CN 91108644 patent/CN1058989A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1322109C (en) * | 2002-10-15 | 2007-06-20 | 湖北安琪酵母股份有限公司 | Drying production technique of fluidized bed by starch adsorption of dry oceanic rhodotorula |
| CN101194011B (en) * | 2005-06-09 | 2012-10-31 | 诺塞斯有限公司 | Dry and/or microencapsulated saccharomyces cerevisiae cells with high content of - (+) -S-adenosyl-L-methionine, process for their preparation and compositions containing them |
| CN101912041A (en) * | 2010-08-02 | 2010-12-15 | 哈尔滨市海澳斯生物科技开发有限公司 | Preparation method of active rhodotorula glutinis powder |
| CN104450380A (en) * | 2014-11-25 | 2015-03-25 | 安琪酵母（睢县）有限公司 | Production method for compound yellow rice wine saccharomycopsis |
| CN104450380B (en) * | 2014-11-25 | 2016-04-27 | 安琪酵母（睢县）有限公司 | Compositional type yellow wine yeast bacterial classification production method |
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| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |