CN105895495A - Ion detection system applicable to laser desorption ionization time-of-flight mass spectrometer - Google Patents
Ion detection system applicable to laser desorption ionization time-of-flight mass spectrometer Download PDFInfo
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- CN105895495A CN105895495A CN201610420960.2A CN201610420960A CN105895495A CN 105895495 A CN105895495 A CN 105895495A CN 201610420960 A CN201610420960 A CN 201610420960A CN 105895495 A CN105895495 A CN 105895495A
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- ion
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- control chip
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/26—Mass spectrometers or separator tubes
- H01J49/34—Dynamic spectrometers
- H01J49/40—Time-of-flight spectrometers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/62—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/02—Details
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- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The invention discloses an ion detection system applicable to a laser desorption ionization time-of-flight mass spectrometer. The ion detection system comprises an ion detector and an acquisition card, wherein the acquisition card comprises a field programmable gate array (FPGA) control chip, an A/D converter, a variable gain amplifier, a DC bias circuit module and a trigger signal input interface, the signal input end of the FPGA control chip is connected with the signal output end of the variable gain amplifier through the A/D converter, and the signal input end of the variable gain amplifier and the signal output end of the DC bias circuit module are connected with the signal output end of the ion detector. The ion detection system has the advantages that the purposes of reducing the fabrication cost of an ion signal detection acquisition system and improving the working stability of the ion signal detection acquisition system are achieved on the condition that the resolution of the laser desorption ionization time-of-flight mass spectrometer is satisfied; and meanwhile, the ion detection system has a function of real-time data uploading, and the problem of data acquisition lag is solved.
Description
Technical field
The present invention relates to Matrix Assisted Laser Desorption ionization time-of-flight mass spectrometer, especially relate to be suitable to the ion detection system of MALDI-TOF-MS instrument.
Background technology
MALDI-TOFMS(Matrix Assisted Laser Desorption ionization time-of-flight mass spectrometer) mass spectrometer system is a kind of novel soft ionization biological mass spectrometry, instrument is mainly made up of two parts: Matrix Assisted Laser Desorption ionization ion source (MALDI) and time of flight mass analyzer (TOF).The principle of MALDI is to irradiate sample and substrate formed cocrystallization film with laser, and matrix absorbs energy from laser and passes to biomolecule；Biomolecule forms fragment after obtaining energy and flies out, and it is a kind of Soft ionization techniques, it is adaptable to mixture and the mensuration of large biological molecule.The principle of TOF is that ion accelerates to fly over tof tube under electric field action, is detected according to the flight time difference arriving ion detector, and capture card given by ion detector after ion signal is amplified, then analyzes qualification ion mass-to-charge ratio later through computer.
The ion signal detection acquisition system of MALDI-TOFMS is as in figure 2 it is shown, be made up of detector 1.1, intermediate amplifier and capture card at present.After ion stream 3.1 arrives ion detector 1.1, ion detector 1.1 exports as an electrical current to intermediate amplifier after being amplified by this signal, intermediate amplifier exports after being amplified by signal to the capture card of voltage range relatively big (typically 5V), and capture card is delivered to computer 2.1 and done subsequent treatment after again continuous print analog signal being converted to discrete data signal.The defect of above-mentioned detection acquisition system is: owing to adding external intermediate signal amplifiers between ion detector 1.1 and capture card, owing to the frequency of signal is higher, bandwidth and noise requirements to intermediate signal amplifiers are the highest, make the manufacturing cost detecting acquisition system the highest, the most also need to provide intermediate signal amplifiers power supply, make to detect acquisition system complicated, add the probability of malfunction of detection acquisition system；The most above-mentioned capture card does not have the real-time upload function of data, causes data acquisition delayed and affects the performance of whole detection acquisition system.
Summary of the invention
Present invention aim at providing a kind of ion detection system being suitable to MALDI-TOF-MS instrument.
For achieving the above object, the present invention takes following technical proposals:
The ion detection system being suitable to MALDI-TOF-MS instrument of the present invention, including ion detector and capture card；Described capture card is made up of FPGA control chip, A/D converter, variable gain amplifier, DC bias circuit module and triggering signal input interface；The signal input part of described FPGA control chip is connected with the signal output part of described variable gain amplifier by A/D converter, and the signal input part of variable gain amplifier and the signal output part of described DC bias circuit module signal output part with described ion detector respectively is connected；The data sending terminal of FPGA control chip is connected with the data receiver of outer computer.
The output control terminal of described FPGA control chip connects status indicator lamp unit.
The invention has the advantages that under conditions of meeting MALDI-TOF-MS instrument resolution ratio, reduce ion signal detection acquisition system manufacturing cost, improve ion signal detection acquisition system job stability purpose, simultaneously, the present invention possesses the real-time upload function of data, solves the problem that data acquisition is delayed.
Accompanying drawing explanation
Fig. 1 is the electrical block diagram of the present invention.
Fig. 2 is the ion signal detection acquisition system electrical block diagram of existing MALDI-TOF-MS instrument.
Detailed description of the invention
As it is shown in figure 1, the ion detection system being suitable to MALDI-TOF-MS instrument of the present invention, including ion detector 1 and capture card；Capture card is by FPGA(FPGA: the abbreviation of English Field-Programmable Gate Array, field programmable gate array) control chip, A/D converter, variable gain amplifier, DC bias circuit module and trigger signal input interface composition；The signal input part of described FPGA control chip is connected with the signal output part of described variable gain amplifier by A/D converter, and the signal input part of variable gain amplifier and the signal output part of described DC bias circuit module signal output part with described ion detector respectively is connected；The data sending terminal of FPGA control chip is connected with the data receiver of outer computer 2 by PCIe bus.Output control terminal in FPGA control chip connects status indicator lamp unit, for showing state (such as acquisition state, idle condition) and the indicating fault (power failure instruction, the instruction of PCIe communication failure) of current capture card.
When the present invention works, the multiplication factor of variable gain amplifier can be made full use of the voltage range of A/D converter by user setup, the signal through amplifying；Analog signal is converted into after digital data transmission processes to FPGA control chip and is put into data buffer area by A/D converter, when computer 2 by PCIe bus request data cached time, data are sent to computer 2 by FPGA control chip.When normally working, the synchronizing signal of MALDI-TOF-MS instrument is transferred to capture card by triggering signal input interface, and capture card starts to gather signal, just data is passed through PCIe bus transfer to computer when data volume reaches to pre-set value.
Claims (2)
1. be suitable to an ion detection system for MALDI-TOF-MS instrument, including ion detector and capture card；It is characterized in that: described capture card is made up of FPGA control chip, A/D converter, variable gain amplifier, DC bias circuit module and triggering signal input interface；The signal input part of described FPGA control chip is connected with the signal output part of described variable gain amplifier by A/D converter, and the signal input part of variable gain amplifier and the signal output part of described DC bias circuit module signal output part with described ion detector respectively is connected；The data sending terminal of FPGA control chip is connected with the data receiver of outer computer.
Be suitable to the ion detection system of MALDI-TOF-MS instrument the most according to claim 1, it is characterised in that: the output control terminal of described FPGA control chip connects status indicator lamp unit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610420960.2A CN105895495A (en) | 2016-06-15 | 2016-06-15 | Ion detection system applicable to laser desorption ionization time-of-flight mass spectrometer |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610420960.2A CN105895495A (en) | 2016-06-15 | 2016-06-15 | Ion detection system applicable to laser desorption ionization time-of-flight mass spectrometer |
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| CN105895495A true CN105895495A (en) | 2016-08-24 |
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| CN201610420960.2A Withdrawn CN105895495A (en) | 2016-06-15 | 2016-06-15 | Ion detection system applicable to laser desorption ionization time-of-flight mass spectrometer |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022021378A1 (en) * | 2020-07-31 | 2022-02-03 | 杭州九洋生物科技有限公司 | Waveguide module for maldi-tof-ms target plate sample preparation and preparation method |
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- 2016-06-15 CN CN201610420960.2A patent/CN105895495A/en not_active Withdrawn
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| US5777326A (en) * | 1996-11-15 | 1998-07-07 | Sensor Corporation | Multi-anode time to digital converter |
| CN101504431A (en) * | 2009-02-20 | 2009-08-12 | 重庆大学 | Nanosecond on-line detection system for random pulse time sequence |
| CN101789355A (en) * | 2010-03-18 | 2010-07-28 | 广州禾信分析仪器有限公司 | Time-of-flight mass spectrometer with wide dynamic range, implementation method and application thereof |
| CN101860657A (en) * | 2010-05-14 | 2010-10-13 | 中国科学院上海技术物理研究所 | Real-time image acquisition method and system of large-view field visible infrared dual-channel camera |
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Application publication date: 20160824 |