CN105884920A - Preparation method of ulva pertusa polysaccharide zinc complex capable of resisting oxidative stress action caused by heavy ion irradiation - Google Patents
Preparation method of ulva pertusa polysaccharide zinc complex capable of resisting oxidative stress action caused by heavy ion irradiation Download PDFInfo
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- CN105884920A CN105884920A CN201410724395.XA CN201410724395A CN105884920A CN 105884920 A CN105884920 A CN 105884920A CN 201410724395 A CN201410724395 A CN 201410724395A CN 105884920 A CN105884920 A CN 105884920A
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- ulva pertusa
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Abstract
The invention relates to a chemical product and a production method of the product. Ulva pertusa is medical seaweed, and the main active ingredient of the ulva pertusa is ulva pertusa polysaccharide. Zinc ion has an import health effect and is particularly well combined with polysaccharides in a human body. According to a preparation method of ulva pertusa polysaccharide zinc with a function of resisting the oxidative stress action caused by heavy ion irradiation, an ultrasonic wave- membrane separation combination method is utilized, so that the ulva pertusa polysaccharide can be adequately realized, the extraction rate can reach 85%-90% and is 10%-20% higher than that of a reflux method or a water extraction method, and the activity is 10%-20% higher than that of the reflux method or the water extraction method; after the complexing of zinc ions, the activity is increased by 20%-30%; the application range of the ulva pertusa polysaccharide is expanded, and the economic additional value of the ulva pertusa polysaccharide is increased. The preparation method is simple in process and is suitable for being generalized and applied. The ulva pertusa polysaccharide prepared by virtue of the preparation method is high in activity and can be taken as the raw material of foods, medicines, health products and cosmetics.
Description
Technical field:
The present invention relates to the production method of a kind of functional product and this product, i.e. U. pertusa there is anti-heavy ion irradiation cause polysaccharide Zn complex and the preparation method thereof of oxidative stress effect.
Background technology:
U. pertusa is a kind of large-scale chlorella, and it is coastal that it is distributed widely in Western Pacific, is a kind of chlorella of yellow Bohai Sea yield maximum.U. pertusa is Medicinal Algae, has anticoagulation, purifies the effects such as blood, antiviral and cholesterol reducing.
At present, U. pertusa polysaccharide has extracted, it is adaptable to and to demonstrate U. pertusa polysaccharide be the important sources that the anti-heavy ion irradiation of U. pertusa causes oxidative stress drug effect.Polysaccharide, refers generally to the polysaccharide that the degree of polymerization is beyond more than 10, and its molecular weight is typically tens thousand of to millions of, is a class natural polymer.Research data proves, polysaccharide has higher medical value and health-care effect, the particularly effect of the pharmaceutically active such as anticoagulation, antithrombotic.The present invention is the metal complex of the U. pertusa polysaccharide causing oxidative stress effect for anti-heavy ion irradiation, improves the nutritive value of U. pertusa, can be used for the raw material of food, medicine, health product, cosmetics.
Summary of the invention:
In order to solve extraction efficiency and the problem of nutritive value of U. pertusa raw material polysaccharide, the present invention uses membrance separation to combine the method drawing holes ulva polysaccharide of ultrasound wave, screen anti-heavy ion irradiation cause oxidative stress effect component and with metallic zinc ion phase complexation, obtain U. pertusa polysaccharide Zn complex, extraction ratio and the Product Activity of U. pertusa polysaccharide can be effectively improved.In the present invention, a kind of have the preparation method that anti-heavy ion irradiation causes the U. pertusa polysaccharide Zn complex of oxidative stress effect, it is characterized in that the step of the method produced of U. pertusa polysaccharide Zn complex is as follows: one. U. pertusa has the screening that anti-heavy ion irradiation causes the polysaccharide of oxidative stress effect: chooses three kinds of U. pertusa polysaccharide laggard row of feed laboratory animal respectively and carries out heavy ion irradiation, filters out and have the polysaccharide component that anti-oxidation stress effect is best.Two. producing of U. pertusa polysaccharide Zn complex: take U. pertusa polysaccharide, be dissolved in water, the zinc ion solution of addition, between tune pH value to 6-7, cool down after slight fever reaction, filter, filtrate is concentrated alcohol analysis, obtaining light gray substance is crude product.Three. purification: by dissolving crude product, dialysis, purify at film, dialysis solution concentration, alcohol are analysed, precipitate, washed, obtains U. pertusa polysaccharide Zn complex.Four, it is dried: vacuum freeze-drying machine 0.5-0.7mbar, temperature-45～-55 DEG C, pulverizing after lyophilizing is 60 mesh, obtains U. pertusa polysaccharide zinc powder.
In the present invention, in the preparation method of the many zinc of U. pertusa there is anti-heavy ion irradiation causing oxidative stress function, use the method that ultrasound wave is combined with membrance separation, U. pertusa polysaccharide is made fully to discharge, extraction ratio can reach 85-90%, 10-20% higher than the extraction ratio of circumfluence method or water extraction, specific activity circumfluence method or water extraction height 10-20%, after complexation zinc ion, specific activity is that complex improves 20-30%.Expand the range of application of U. pertusa polysaccharide, improve the economic value added of U. pertusa polysaccharide.Present invention process is simple, is suitable to promotion and application.U. pertusa polysaccharide zinc activity prepared by the present invention is high, can be as food, medicine, health product, the raw material of cosmetics.
Detailed description of the invention:
Detailed description of the invention one: in this detailed description of the invention, a kind of have the preparation method that anti-heavy ion irradiation causes the U. pertusa polysaccharide Zn complex of oxidative stress effect, it is characterized in that the step of the method produced of U. pertusa polysaccharide Zn complex is as follows: one. U. pertusa has the screening that anti-heavy ion irradiation causes the polysaccharide of oxidative stress effect: chooses three kinds of U. pertusa polysaccharide laggard row of feed laboratory animal respectively and carries out heavy ion irradiation, filters out and have the polysaccharide component that anti-oxidation stress effect is best.Two. producing of U. pertusa polysaccharide Zn complex: take U. pertusa polysaccharide, be dissolved in water, the zinc ion solution of addition, between tune pH value to 6-7, cool down after slight fever reaction, filter, filtrate is concentrated alcohol analysis, obtaining light gray substance is crude product.Three. purification: by dissolving crude product, dialysis, purify at film, dialysis solution concentration, alcohol are analysed, precipitate, washed, obtains U. pertusa polysaccharide Zn complex.Four, it is dried: vacuum freeze-drying machine 0.5-0.7mbar, temperature-45～-55 DEG C, pulverizing after lyophilizing is 60 mesh, obtains U. pertusa polysaccharide zinc powder.
Detailed description of the invention two: this detailed description of the invention is unlike detailed description of the invention one: in step one, heavy ion irradiation uses the dosage 20-200mGry that simulation airship orbits the earth 15 days, applies heavy ion avcceleration12C6+Bundle irradiates, and selected polysaccharide is cold water extraction part.Other are identical with detailed description of the invention one.
Detailed description of the invention three: this detailed description of the invention is unlike detailed description of the invention one: in step one, its ultrasonic intensity is 75 kilo hertzs～90 kilo hertzs, and plate and frame membrance separation rank is 6000-10000 part, and in gained concentrated solution, solid content is 50-70%.Other are identical with detailed description of the invention one.
Detailed description of the invention four: this detailed description of the invention is unlike detailed description of the invention one: adjusting pH value between 7-8 with the NaOH of 0.5mol/L in step 2, slight fever is reacted 6-8 hour.Other are identical with detailed description of the invention one.
Detailed description of the invention five: this detailed description of the invention is unlike detailed description of the invention one: the film selected by step 3 is plate and frame filter membrane, membrance separation rank is 8000-10000.Other are identical with detailed description of the invention one.
Detailed description of the invention six: this detailed description of the invention is unlike detailed description of the invention one: the external spectrum characteristic peak data of the U. pertusa polysaccharide Zn complex described in step 3 are 3450-3300 (-OH), 2926-2847 (-CHZ-), 1607-1621 (-C=O), 1159-1132 (-C-O-C), 1098-1015 (-C-O-H).Other are identical with detailed description of the invention one.
In this detailed description of the invention, the combination rate of U. pertusa polysaccharide zinc is 82-91%, and purity is 70-80%, and gained U. pertusa many zinc activity is high, quality better.
Claims (6)
1. one kind has anti-heavy ion irradiation and causes the producing of U. pertusa polysaccharide Zn complex of oxidative stress effect
Method, it is characterised in that it is realized by following step:
(1) screening: choose three kinds of U. pertusa polysaccharide laggard row of feed laboratory animal respectively and carry out heavy ion irradiation,
Filter out and there is the polysaccharide component that anti-oxidation stress effect is best；
(2) produce: take U. pertusa polysaccharide, be dissolved in water, the zinc ion solution of addition, tune pH value to 6-7
Between, cool down after slight fever reaction, filter, filtrate is concentrated alcohol analysis, obtaining light gray substance is crude product；
(3) purification: by dissolving crude product, dialysis, purify at film, dialysis solution is concentrated, alcohol analysis, precipitation,
Washing, obtains U. pertusa polysaccharide Zn complex；
(4) being dried: vacuum freeze-drying machine 0.5-0.7mbar, temperature-45～-55 DEG C, pulverizing after lyophilizing is 60 mesh,
Obtain U. pertusa polysaccharide zinc powder.
U. pertusa the most according to claim 1 has the screening technique of the polysaccharide of radiation resistance, and it is special
Levy and be in step (1) that irradiation uses the dosage 20-200mGry that simulation airship orbits the earth 15 days, should
Use heavy ion avcceleration12C6+Bundle irradiates, and selected polysaccharide is cold water extraction part.
The extracting method of U. pertusa polysaccharide the most according to claim 1, it is characterised in that in step (2)
Its ultrasonic intensity is 75 kilo hertzs～90 kilo hertzs, and plate and frame membrance separation rank is 6000-10000 part, institute
Obtaining solid content in concentrated solution is 50-70%.
The preparation method of U. pertusa polysaccharide Zn complex the most according to claim 1, it is characterised in that step
Suddenly the adjusting PH with base value of (2) middle 0.5mol/L is between 7-8, and slight fever is reacted 6-8 hour.
The purification process of U. pertusa polysaccharide Zn complex the most according to claim 1: it is characterized in that step
Suddenly the film selected by (3) is plate and frame filter membrane, and membrance separation rank is 8000-10000.
The sign of U. pertusa polysaccharide Zn complex the most according to claim 1, it is characterised in that step (3)
The external spectrum characteristic peak data of described U. pertusa polysaccharide Zn complex are 3450-3300 (-OH),
2926-2847 (-CHZ-), 1607-1621 (-C=O), 1159-1132 (-C-O-C), 1098-1015 (-C-O-H).
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107286267A (en) * | 2017-07-27 | 2017-10-24 | 福建农林大学 | One Polysaccharides From Laminaria Japonica Zn complex and its preparation and application |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107286267A (en) * | 2017-07-27 | 2017-10-24 | 福建农林大学 | One Polysaccharides From Laminaria Japonica Zn complex and its preparation and application |
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Address after: 150001 School of Chemical Engineering and Chemistry, No.92 Xidazhi Street, Nangang District, Harbin City, Heilongjiang Province Applicant after: Lu Weihong Address before: 150090 2649 the Yellow River Road, Nangang District, Harbin, Heilongjiang, 2649 Applicant before: Lu Weihong |
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