CN105884475A

CN105884475A - Mixture medium for edible mushrooms and making method thereof

Info

Publication number: CN105884475A
Application number: CN201410445842.8A
Authority: CN
Inventors: 王钰
Original assignee: Individual
Current assignee: Individual
Priority date: 2014-09-03
Filing date: 2014-09-03
Publication date: 2016-08-24

Abstract

The invention discloses a mixture medium for edible mushrooms and a making method thereof. the mixture medium contains various different types and mass of raw materials and is characterized by comprising the following ingredients (by weight): 40-50% of grain cereal granules, 30-40% of broad-leaved sawdust, 15-20% of wheat bran or rice chaff, 2% of bean flour and 1% of gypsum. The mixture medium has the following beneficial effects: the raw materials are easily available, and nutrients are rich. The mixture medium is ageing-resistant in comparison with a pure grain cereal medium or a test-tube agar medium, and has high survival rate of mother culture in comparison with sawdust, wood residue and agar mediums. The production method is simple and is easy to operate. The mixture medium has a wide range of application and is beneficial to industrial promotion and application.

Description

A kind of edible fungi mixture culture medium and preparation method thereof

Technical field

The present invention relates to culture medium of edible fungus technical field, particularly relate to one and can be used for edible fungus culturing The manufacture method of compound culture medium.

Background technology

Edible fungi refers to that sporophore is very large, mushroom fungus (macro fungi) that can be edible, is commonly referred to as mushroom. The known edible fungi of China has kind more than 350, wherein belongs to Basidiomycotina more, common are: Lentinus Edodes, grass Mushroom, mushroom, Auricularia, Tremella, hedgehog hydnum, Caulis Bambusae In Taeniam, Trichotoma matsutake (Tricholoma matsutake (lto et lmai) Singer), Tricholoma mongolicum Imai, Russula and Hepar Bovis seu Bubali Bacterium etc.；Minority belongs to Ascomycotina, Qi Zhongyou: Morchella esculenta (L.) Pers, saddle fungus, truffles etc..Above-mentioned fungus It is grown in respectively in different areas, different ecological environmenies.Since the eighties initial stage, edible fungi is planted Train that investment is little, the cycle is short, the project of having got rich of instant effect is able to fast development in China as one, eat With bacterium product, the most once supply falls short of demand, and selling price is high.Mushroom industry is collection economic benefit, an ecology Benefit and social benefit in the short, adaptable and fast rural economic development project of one, edible fungi be again a class organic, Nutrition, the pollution-free food of health care.Development mushroom industry meets people and consumes growth and agricultural sustainable development The needs of exhibition, are the effective ways quickly got rich of peasant.It is main that 21 century edible fungi will develop into the mankind One of protein food, the total output of edible fungi of china in 2005 reaches 12,000,000 tons, ranks first in the world, The total output of edible fungi of china in 2010 reaches 20,000,000 tons, accounts for the world 70%.During mushroom industry becomes An important industry in state's plant husbandry, domestic market has a high potential.

Edible fungi is also that a kind of people like eating simultaneously, and the vegetable food being of high nutritive value, natural open country Though raw edible fungi is fond of eating, but because of weather or the restriction of geographical conditions, this wild edible fungi must be competing Seldom, it is impossible to meet the consumption demand of numerous town dwellers.Therefore, current Vegetable Market disappears the food sold With bacterium, typically all artificial culture out, its method is that the edible fungus species of different cultivars is transplanted to In the culture medium of multiple different materials, under the conditions of manually operated temperature humidity, its growth promoter is allowed to become Different edible fungi；The culture medium of edible fungus that people use in the past, is to use cotton seed hulls mostly, cotton stem, Brassica campestris L shell or other plant fiber, owing to these raw materials are inherently few, add that the people of use is many, cause Culture medium of edible fungus raw material is nervous, and price constantly rises；Some uses pure grain grain culture medium And test tube agar culture medium, this method culture medium relatively costly, cause bigger waste, and cultivate Base is the most aging, and the holding time is short；Also part is had to use wood flour and wood to squeeze as culture medium, but this side Method, parent edible fungus kind survival rate is relatively low.

In order to solve the various technical bottlenecks in culture medium of edible fungus production field, the present invention proposes one Edible fungi mixture culture medium manufacture method, its manufacture method is simple, and raw material is easy to get, and female survival rate of planting is high, Holding time is long, can meet the actual demand of food bacterium enterprise, fills up correlation technique blank, can produce again Raw bigger social benefit and economic benefit.

Summary of the invention

In order to overcome above-mentioned the deficiencies in the prior art, the invention provides a kind of edible fungi mixture culture medium Manufacture method, it is possible to increase parent edible fungus kind survival rate, the holding time of Extending culture base, reduces and cultivates The cost of manufacture of base and difficulty of drawing materials, be suitable for large-scale industrial production, in order to improve culture medium raw material Utilization rate and product bacterium rate, reduce Edible Fungi cost, simplified cultivation technology, promote culture medium of edible fungus Technology advances.

The technical solution adopted for the present invention to solve the technical problems is: creative utilizing is multiple common former Material mix and match is quantitatively modulated compound and is cultivated base, can be used for the cultivation of edible fungi.The present invention proposes one Compound is used to make optimization of C/C composites and the cultivation technique of culture medium of edible fungus, the compound that the present invention uses The composition of culture medium and percentage by weight are respectively as follows: foodstuff grain granule 40-50%, broad-leaved saw foam 30- 40%, Testa Tritici or rice chaff 15-20%, Semen Glycines powder 2%, Gypsum Fibrosum 1%.

The cultural method that the present invention uses, it includes step specific as follows:

1, get the raw materials ready: get all the whole culture medium raw materials ready by above-mentioned raw materials proportioning.

2, mixing: by foodstuff grain granule, broad-leaved saw foam, Testa Tritici or rice chaff are sufficiently mixed, and stir.

3, solution: by Semen Glycines powder, Gypsum Fibrosum is put into stirring in water and is dissolved, makes Gypsum Fibrosum and Semen Glycines powder aqueous solution.

4, fermentation: by the Gypsum Fibrosum modulated and Semen Glycines powder aqueous solution to entering mixed foodstuff grain granule, wealthy Leaf saw foam, in Testa Tritici or rice chaff, after stirring, carries out heap fermentation by every square metre of windrow 40kg, Turn over a heap for every eight hours, when temperature of turning sour reaches 100 DEG C, stop fermentation.

5, sterilizing: by the raw material after by fermentation, pack with plastic bag, permanent at a temperature of 100 DEG C Temperature keeps 8 hours, reaches sterilization purpose.

6, inoculation: edible fungus species is implanted the two ends of the plastic bag after sterilizing, is then placed in preparing in advance In good hot house, keeping temperature 25 DEG C in canopy, humidity is maintained at 80%, keeps health in canopy, anti- Only insect poison and miscellaneous bacteria, the edible fungi grown after 26 days just can come into the market.

Compared with prior art, the invention has the beneficial effects as follows: 1, raw material is easy to get, wide material sources, cultivate Base is easily manufactured, nutritious, comprehensively, mycelial growth preferably, test tube agar culture medium can be replaced to use In two grades of bacterium of switching, and do not affect the kind of strain, be suitable for factorial praluction；2, ratio pure grain corn Culture medium and test tube agar culture medium are ageing-resistant, and after mycelia covers with bottle, the holding time is long, 10-20 ° of room temperature Can preserve more than three months and never degenerate；3, ratio wood flour, it is high that wood slag and agar culture medium mother plant survival rate, Under conditions of two grades of bacterium culture medium moisture content are suitable, survival rate can be really achieved absolutely, during two grades of bacterium of inoculation Only access one or several compound mother to plant granule and just can survive, the field planting in 24 hours when 25-28 °, And material feeding is fast, and mycelia is sturdy effectively；4, production method is simple, it is easy to operation, beneficially popularization and application； 5, applied widely, the culture medium of this compound preparation, multiple eating bacterium can be produced, experiments verify that, It is suitable for the cultivating and growing of the edible fungi such as chicken foot mushroom, Flammulina velutiper (Fr.) Sing, Lentinus Edodes and Pleurotus ostreatus.

Detailed description of the invention

Below in conjunction with embodiment, the present invention is further described, embodiment: with compound culture medium cultivation gold The method of pin mushroom, it comprises the steps:

1, get the raw materials ready: get all the following raw material cultivating base, corn 40-50%, broad-leaved saw foam by weight ratio ready 30-40%, rice chaff 15-20%, Semen Glycines powder 2%, Gypsum Fibrosum 1%.

2, mixing: by corn, broad-leaved saw foam, rice chaff is sufficiently mixed, and stirs.

3, solution: by Semen Glycines powder, Gypsum Fibrosum is put into and is sufficiently stirred in water dissolving, and makes Gypsum Fibrosum and Semen Glycines powder aqueous solution.

4, fermentation: the Gypsum Fibrosum modulated and Semen Glycines powder aqueous solution are imported mixed corn, and broad-leaved saws foam, In rice chaff, after stirring, carry out heap fermentation by every square metre of windrow 40kg, turn over for every eight hours once Heap, when temperature of turning sour reaches 100 DEG C, stops fermentation.

5, sterilizing: by the raw material after by fermentation, pack with plastic bag, every bag of 1kg, at 100 DEG C At a temperature of constant temperature keep 8 hours, reach sterilization purpose.

6, inoculation: after the culture medium the first day of the lunar month pocket after subject to sterilization is cooled to room temperature, implants edible fungus species and goes out The two ends of the plastic bag after bacterium, are then placed in preprepared hot house, keep temperature in canopy 25 DEG C, humidity is maintained at 80%, keeps health in canopy, prevents insect poison and miscellaneous bacteria, the food grown after 26 days Just can come into the market with bacterium, the plastic bag culture medium of 1kg about can grow 5kg edible fungi.

The above, be only the better embodiment of invention, the present invention not do any restriction, every Any amendment, change and equivalence change embodiment of above made according to essence of the present invention, the most still Within belonging to the protection domain of the technology of the present invention.

Claims

1. edible fungi mixture culture medium and preparation method thereof, including multiple variety classes and the raw material of quality, It is characterized in that composition and the percentage by weight of compound culture medium are respectively as follows: foodstuff grain granule 40- 50%, broad-leaved saw foam 30-40%, Testa Tritici or rice chaff 15-20%, Semen Glycines powder 2%, Gypsum Fibrosum 1%.

A kind of edible fungi mixture culture medium the most according to claim 1 and preparation method thereof, its feature exists In by foodstuff grain granule, broad-leaved saw foam, Testa Tritici or rice chaff are sufficiently mixed, and stir.

A kind of edible fungi mixture culture medium the most according to claim 1 and preparation method thereof, its feature exists In by Semen Glycines powder, Gypsum Fibrosum is put into stirring in water and is dissolved, and makes Gypsum Fibrosum and Semen Glycines powder aqueous solution.

A kind of edible fungi mixture culture medium the most according to claim 1 and preparation method thereof, its feature exists Importing mixed corn in the Gypsum Fibrosum that will modulate and Semen Glycines powder aqueous solution, broad-leaved saws foam, in rice chaff, After stirring, carry out heap fermentation by every square metre of windrow 40kg, turn over a heap for every eight hours, when When temperature of turning sour reaches 100 DEG C, stop fermentation.

A kind of edible fungi mixture culture medium the most according to claim 1 and preparation method thereof, its feature exists In by the raw material after by fermentation, packing with plastic bag, at a temperature of 100 DEG C, constant temperature keeps 8 Hour, reach sterilization purpose.

A kind of edible fungi mixture culture medium the most according to claim 1 and preparation method thereof, its feature exists The two ends of the plastic bag after edible fungus species is implanted sterilizing, are then placed in preprepared plastics In booth, keeping temperature 25 DEG C in canopy, humidity is maintained at 80%, keeps health in canopy, prevents insect poison And miscellaneous bacteria.