CN105866450A - Micro-fluid test card - Google Patents
Micro-fluid test card Download PDFInfo
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- CN105866450A CN105866450A CN201610225192.5A CN201610225192A CN105866450A CN 105866450 A CN105866450 A CN 105866450A CN 201610225192 A CN201610225192 A CN 201610225192A CN 105866450 A CN105866450 A CN 105866450A
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- fluid reservoir
- pump chamber
- sealant
- reative cell
- layers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
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- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention discloses a micro-fluid test card. The test card comprises reaction chamber and micro-channel layers, micro-channel sealing layers ad detection layers, and further comprises at least one liquid storage and pump chamber projecting out of the reaction chamber and micro-channel layers, first liquid storage chamber sealing layers arranged between every liquid storage chamber and pump chamber and micro-channels and used for isolating the at least one liquid storage chamber and pump chamber, and waste liquid pools projecting out of the reaction chamber and micro-channel layers and connected with a reaction chamber. The micro-fluid test card adopting the above technical scheme can greatly reduce the volatility of a liquid, so the preservation time of the test card is prolonged, and loss of the test card is reduced.
Description
Technical field
The present invention relates to micro-fluidics bio detection, particularly relate to one can freely assembled, can arbitrarily control instead
Answer flow velocity, prevent from storing the microfluid test card that liquid leaks outside.
Background technology
Owing to using the development of the micro-fluidic technologies of micro solution, the lab-on-a-chip being (LOC) or miniflow
The proud constraint departing from tradition research experiment of the purposes of body chip, further marches toward and relatively has a practical value
In business application.In the field such as chemical, biological, microfluid system is more and more applied, such as trace
The transmission of fluid, quantity compou nd synthesis, sample separation, constituent analysis and chemical reaction.
Micro-fluid chip is based on electrophoresis chip at present, and the injection of reaction reagent is to realize by electrophoresis, electric osmose,
Although the purpose of trace fluid injection can be realized, but poor repeatability.Although and using the method letter that syringe injects
Single, but the amount of solution used is many, often has the waste of reagent.For accurate micro-sampling problem, special
It not the problem of multiple solution order sample introduction, extensively paid close attention to by industry personnel.
At present, although have some micro-fluidic technologies to have been put into market, but, still suffer from problems not yet
Solve, such as:
The patent No. " 200710198600.3 " a kind of has solution storage chamber and holds concurrently the microfluid sample boat of pump body structure.
Although the problem overcoming trace sequential injection, and fluid reservoir and pump chamber are merged into a single whole.But, this is special
There is following problem in profit: 1) structure of program middle pump body is complicated, is unfavorable for industrial production and finished product
Assemble；2) flow velocity of liquid in microchannel cannot be controlled by the program, it is impossible to according to the actual need of user
Flow rate of liquid in microchannel to be adjusted, controls reaction speed.
The microfluid sample boat of the patent No. " 200710175624.7 ".Although overcoming asking of trace sequential injection
Topic, but, the structure still using many liquid pools liquid storage with entering liquid and liquid storage separates, these complexity structure is set
Waste use space greatly, add the processing cost of example boat.Meanwhile, for sample cell,
The liquid tight seal of liquid storage tank and liquid inlet does not has any measure, easily under unused state, due to external force or
The reasons such as concussion make the liquid of storage leak, and cause example boat to be scrapped.
In sum, prior art existing following problem mostly: 1) structure is relative complex, it is unfavorable for
Produce and assemble；2) flow rate of liquid cannot be needed to control by user；3) storage liquid of avoiding that cannot be good is let out
The problem of leakage.
Accordingly, it is desirable to provide a kind of novel microfluid test card, with meet user to the security of test card,
High efficiency and the demand of low cost.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of microfluid test card, to solve to tie in prior art
Structure is relative complex, is unfavorable for producing and assembling；Flow rate of liquid cannot be needed to control by user；Cannot be good
The problem avoiding storing liquid leakage.
For solving above-mentioned technical problem, the present invention uses following technical proposals:
A kind of microfluid test card, this test card includes reative cell and microchannel layers, micro-channels layer and inspection
Survey layer,
This microfluid test card farther includes:
Externally protrude out at least one fluid reservoir of reative cell and microchannel layers to hold concurrently pump chamber；
For sealing the first fluid reservoir sealant of microchannel that fluid reservoir holds concurrently pump chamber and isolation can connect；
Be positioned at described first fluid reservoir sealant opposite side for seal fluid reservoir hold concurrently pump chamber second liquid storage seal
Layer；
Waste liquid pool, externally protrudes out in reative cell and microchannel layers and connects with reative cell；And,
It is positioned at the pump chamber sealant outside described second liquid storage sealant.
Preferably, the material of described second fluid reservoir sealant includes aluminum film layer, it is preferable that described first
The material of fluid reservoir sealant includes aluminum film layer.
Preferably, the material of described second fluid reservoir sealant farther include described aluminum film layer surface with
The etch resistant material layer of described pump chamber sealant opposite side, it is preferred that the material of described first fluid reservoir sealant
Material farther includes described aluminum film layer surface with described pump chamber sealant homonymy etch resistant material layer.
Preferably, the hold concurrently sidewall of pump chamber of described fluid reservoir is rigid material, and described pump chamber sealant is elastic material
The bed of material.
Preferably, described fluid reservoir is held concurrently the sidewall elastomeric material of pump chamber, and described pump chamber sealant is rigid material
Layer.
Preferably, the hold concurrently sidewall of pump chamber of described fluid reservoir is structure as a whole with reative cell and microchannel layers.
Preferably, be sealed with the fluid reservoir of the first and second fluid reservoir sealants hold concurrently pump chamber seal be assembled in described
On reative cell and microchannel layers.
Preferably, described fluid reservoir holds concurrently sidewall and the reative cell of pump chamber and microchannel layers uses identical or different
Make material.
Preferably, described detection layers is to be provided with the printed circuit board (PCB) of magnetosensitive chip.
Preferably, described detection layers is to be provided with the fluorescence inspection for reactant liquor in reative cell carries out biochemistry detection
Survey agent.
Preferably, described detection layers or reative cell and microchannel layers are transparent material.
Preferably, described micro-channels layer offers the opening corresponding with described reative cell；
Described detection layers be provided with the carrying detection chip for reactant liquor in reative cell being carried out biochemistry detection or
The detection zone of detection agent；
In reative cell on described reative cell and microchannel layers, the opening on micro-channels layer and detection layers
Detection zone alignment compress in the case of, fix successively reative cell and microchannel layers, micro-channels layer and
Detection layers, forms a test card with the sealing of reagent entry port, fluid passage and reative cell
Beneficial effects of the present invention is as follows:
Fluid reservoir and pump chamber are united two into one by technical scheme of the present invention, and logical with micro-at fluid reservoir pump chamber of holding concurrently
Be provided with between road one layer can the most rupturable film, this design not only can guarantee that fluid reservoir
Holding concurrently the security seal of pump chamber can rupturing under external force, the liquid making fluid reservoir hold concurrently in pump chamber flows into micro-leading to
Road.The deformation occurred under external force by pump body structure described in this programme, promotes fluid reservoir to hold concurrently pump chamber
Interior liquid flows into microchannel or described elaxtic seal deforms upon under external force, promotes miniflow to lead to
Liquid in road accelerates flowing, it is achieved to the conveying of liquid in microchannel and flow control.
This programme is by being sealed with the first of the composite membrane being made up of aluminium and elastomeric material on holding concurrently pump chamber at fluid reservoir
Liquid storage sealant and the second liquid storage sealant, make indoor liquid volatility reduce, thus the guarantor of extended testing system card
Deposit the time, reduce the loss of test card.Existing test card is only capable of depositing for 2 to 3 weeks, uses the survey of this programme
Examination card can deposit at least year.
In this programme, use fluid reservoir pump chamber of holding concurrently detachably connectable with reative cell microchannel layers, the most convenient use
Family according to liquid storage character need change fluid reservoir and hold concurrently the material of pump chamber.In this programme, detection layers uses transparent
Or translucent inorganic material, and fluoroscopic examination district is set thereon, can further biochemical reaction be carried out
Fluoroscopic examination, has widened the detection range of conventionally test card.
Accompanying drawing explanation
Below in conjunction with the accompanying drawings the detailed description of the invention of the present invention is described in further detail；
Fig. 1 illustrates the schematic diagram of a kind of microfluid of the present invention test card；
Fig. 2 illustrates the schematic diagram of microfluid test card described in the embodiment of the present invention 1；
Fig. 3 illustrates reative cell described in the embodiment of the present invention 1 and the schematic diagram of microchannel layers；
Fig. 4 illustrates the schematic diagram held concurrently pump chamber sealant described in the embodiment of the present invention 1 and fluid reservoir pump chamber seals；
Fig. 5 illustrates the schematic diagram of detection layers described in the embodiment of the present invention 1；
Fig. 6 illustrates reative cell described in the embodiment of the present invention 2 and the schematic diagram of microchannel layers；
Fig. 7 illustrates the schematic diagram of detection layers described in the embodiment of the present invention 3.
Drawing reference numeral
10,50,70 reative cell and microchannel layers；20,80 micro-channels layer；30,90 detection layers；40、
85 pump chamber sealants；101,60,701 fluid reservoirs are held concurrently pump chamber；102 aluminum film layers；103 microchannels；104、
501 waste liquid pools；105 reative cells；201 openings；301 wires；302 magnetosensitive chips；303PCB plate；
Detailed description of the invention
In order to be illustrated more clearly that the present invention, below in conjunction with preferred embodiments and drawings, the present invention is done further
Explanation.Parts similar in accompanying drawing are indicated with identical reference.Those skilled in the art should
Work as understanding, below specifically described content be illustrative and be not restrictive, this should not limited with this
The protection domain of invention.
As it is shown in figure 1, the invention discloses a kind of microfluid test card, this test card includes reative cell and micro-
Channel layer, micro-channels layer and detection layers；This microfluid test card farther includes: externally protrude out in reaction
At least one fluid reservoir of room and microchannel layers is held concurrently pump chamber；For seal fluid reservoir hold concurrently pump chamber and isolation can connect
First fluid reservoir sealant of logical microchannel；It is positioned at described first fluid reservoir sealant opposite side for sealing
Fluid reservoir is held concurrently the second liquid storage sealant of pump chamber；Waste liquid pool, externally protrude out in reative cell and microchannel layers and with instead
Room is answered to connect；And, it is positioned at the pump chamber sealant outside described second liquid storage sealant.
In this programme, described first fluid reservoir sealant and the second fluid reservoir sealant are can be under external force
The film ruptured；The material of described second fluid reservoir sealant includes aluminum film layer, it is preferable that described first
The material of fluid reservoir sealant includes aluminum film layer.The material of described second fluid reservoir sealant farther includes
The etch resistant material layer with described pump chamber sealant opposite side on described aluminum film layer surface, it is preferred that described
The material of the first fluid reservoir sealant is that farther include described aluminum film layer surface with described pump chamber sealant
The etch resistant material layer of homonymy.In this programme, described first fluid reservoir sealant and the second liquid storage sealant exist
Seal fluid reservoir hold concurrently pump chamber time, the etch resistant material layer of two fluid reservoir sealants holds concurrently pump chamber towards fluid reservoir,
Now, the liquid allowing fluid reservoir hold concurrently in pump chamber contacts with resistant material, prevents liquid corrosion aluminum film layer.
The material of this resistant to liquid corrosion can be polypropylene, polystyrene or be similarly used for protecting aluminium film not by liquid
The material of body corrosion.This aluminum film layer with etch resistant material layer is utilized to seal the side of liquid storage indoor liquid
Case, can be substantially reduced the volatility of liquid, and volatility is reduced in prior art test card volatility
1/30, thus the holding time of extended testing system card, reduce the loss of test card.
The pump chamber of holding concurrently of fluid reservoir described in this programme can be two kinds of structures.The first structure is: described fluid reservoir
Double pump chamber is that rigid material is made, and described pump chamber sealant is elastomeric layer, and this elastomeric layer can be
Silicon rubber PDMS.The pump chamber sealant that elastomeric material is made is positioned on the second liquid storage sealant.So design
Pump chamber of can holding concurrently fluid reservoir carries out secondary seal.Pump chamber sealant can also be made to occur under external force
Deformation, extruding fluid reservoir is held concurrently the liquid in pump chamber so that it is flow into microchannel；Or, described pump chamber sealant
Deform upon under external force, make the liquid in microchannel accelerate flowing, it is achieved to liquid in microchannel
Conveying and flow control.The second structure is: described fluid reservoir pump chamber of holding concurrently is that elastomeric material is made, described
Pump chamber sealant is rigid material layer.The pump chamber sealant that elastomeric material is made is positioned at the second liquid storage sealant
On.So design pump chamber of fluid reservoir can being held concurrently and carry out secondary seal.Storage can also be made under external force
Liquid chamber pump chamber of holding concurrently deforms upon, and extruding fluid reservoir is held concurrently the liquid in pump chamber so that it is flow into microchannel；Or,
Described fluid reservoir pump chamber of holding concurrently deforms upon under external force, makes the liquid in microchannel accelerate flowing, real
Now to the conveying of liquid in microchannel and flow control.
In this programme, for the detection of solution in reative cell in complex reaction room and microchannel layers, described micro-
Offering the opening corresponding with described reative cell in passage sealing layer, meanwhile, described detection layers is provided with use
In to reative cell, reactant liquor carries out biochemistry detection and carries the detection zone of detection chip or detection agent.Described
Reative cell on reative cell and microchannel layers, the opening on micro-channels layer and the detection zone in detection layers
In the case of alignment compresses, fix reative cell and microchannel layers, micro-channels layer and detection layers successively,
Formed one with the test card of the sealing of reagent entry port, fluid passage and reative cell, further ensure
Test blocks sealing and the security of each working region.
In this programme, can select in the side, close microchannel that described fluid reservoir is held concurrently between pump chamber and microchannel
Reative cell and microchannel layers are provided with a sharp portion, and user utilizes sharp portion will be arranged on fluid reservoir and holds concurrently on pump chamber
The first fluid reservoir sealant and the second fluid reservoir sealant puncture, held concurrently pump chamber by fluid reservoir in this programme
Structure apply external force, make the liquid that fluid reservoir holds concurrently in pump chamber flow into microchannel.This programme can also select not
Test card in sharp portion is set, it is only necessary to user by fluid reservoir hold concurrently pump chamber structure applying suitable outside
Power, the liquid making fluid reservoir hold concurrently in pump chamber produces pressure to the first fluid reservoir sealant and the second fluid reservoir sealant
Power, thus break through the first fluid reservoir sealant and the second fluid reservoir sealant, and make fluid reservoir hold concurrently in pump chamber
Liquid flows into microchannel.
In this programme, described fluid reservoir pump chamber of holding concurrently can be structure as a whole with reative cell and microchannel layers, it is possible to
Being to be sealed with the fluid reservoir of the first and second fluid reservoir sealants pump chamber of holding concurrently to seal and be assembled in described reative cell
And the separable assembled structure in microchannel layers.In this programme, can be according to the needs of user's liquid storage, by described
Fluid reservoir holds concurrently sidewall and the reative cell of pump chamber and microchannel layers uses identical or different material to make.
In this programme, described detection layers can be the printed circuit board (PCB) being provided with magnetosensitive chip.This test card is permissible
According to actually detected needs, magnetosensitive chip is replaced with the biochemical sensor for detecting other characteristics, and leads to
Cross wire to export data to external equipment.Described detection layers can also be provided with for reactant liquor in reative cell
Carry out the Fluorescent detector of biochemistry detection.When selecting to carry out fluoroscopic examination, need to select transparent inorganic material
Material makes detection layers or reative cell and microchannel layers, so that detection light can allow outside through test card
Equipment Inspection changes to fluoroscopic examination.
When user uses test card, according to reaction needs, the fluid reservoir of different liquids is had to hold concurrently pump to storage successively
Room applies external force, makes fluid reservoir hold concurrently pump chamber or pump chamber sealant deforms upon, promote liquid storage by this deformation
The liquid inflow held concurrently in pump chamber in room is micro-logical, liquid and the reactant reaction being arranged in microchannel or reative cell
After, by detection layers reacted liquid carried out corresponding biochemistry detection, and by the waste liquid row after detection
Enter waste liquid pool.When user needs to accelerate reaction speed time, hold concurrently to fluid reservoir and apply external force on pump chamber, make
Fluid reservoir holds concurrently pump chamber or pump chamber sealant deforms upon, and accelerates the liquid in microchannel by this deformation and flows,
Thus accelerate the reaction speed in test card and test.
Below by several groups of embodiments, the present invention will be further described:
Embodiment 1
As shown in Figures 2 to 4, the invention discloses a kind of microfluid test card, this test card includes reaction
Room and microchannel layers 10, micro-channels floor 20 and detection layers 30；This microfluid test card bag further
Include: externally protrude out at least one fluid reservoir of reative cell and microchannel layers 10 and hold concurrently pump chamber 101；For sealing storage
The first fluid reservoir sealant of microchannel 103 that liquid chamber holds concurrently pump chamber 101 and isolation can connect；It is positioned at described
First fluid reservoir sealant opposite side is held concurrently the second liquid storage sealant of pump chamber 101 for sealing fluid reservoir；Waste liquid
Pond 104, externally protrudes out in reative cell and microchannel layers 10 and connects with reative cell 105；And, it is positioned at described
Pump chamber sealant 40 outside second liquid storage sealant.Described microchannel 103 and reative cell 105 are positioned at reaction
Room and the same side of microchannel layers 10, described fluid reservoir holds concurrently pump chamber 101 and waste liquid pool 104 externally protrude out in instead
Answer in reative cell and the microchannel layers 10 of room 105 opposite side.
In this example, described first fluid reservoir sealant and the second fluid reservoir sealant are can be under external force
The film ruptured；The material of described second fluid reservoir sealant includes aluminum film layer 102, it is preferable that described
The material of the first fluid reservoir sealant includes aluminum film layer 102.The material of described second fluid reservoir sealant enters
One step includes described aluminum film layer 102 surface with described pump chamber sealant 40 opposite side resistant material
Layer, it is preferred that the material of described first fluid reservoir sealant farther includes described aluminum film layer 102 surface
The etch resistant material layer with described pump chamber sealant 40 homonymy.In this programme, described first fluid reservoir is close
Sealing and the second liquid storage sealant seal fluid reservoir hold concurrently pump chamber 101 time, the corrosion resistant of two fluid reservoir sealants
Corrosion material layer is held concurrently pump chamber 101 towards fluid reservoir, now, allows fluid reservoir hold concurrently the liquid in pump chamber 101 and corrosion resistant
Corrosion material contacts, and prevents liquid corrosion aluminum film layer.The material of this resistant to liquid corrosion can be polypropylene, gather
Styrene or be similarly used for protect aluminium film not liquid body corrosion material.
The pump chamber 101 of holding concurrently of fluid reservoir described in this example can be two kinds of structures.The first structure is: described storage
Liquid chamber pump chamber 101 of holding concurrently is made for rigid material, and described pump chamber sealant 40 is elastomeric layer.Elastic material
The pump chamber sealant 40 that material is made is positioned on the second liquid storage sealant.So design pump of fluid reservoir can being held concurrently
Room 101 carries out secondary seal.Pump chamber sealant 40 can also be made to deform upon under external force, squeeze
Pressure fluid reservoir is held concurrently the liquid in pump chamber 101 so that it is flow into microchannel 103；Or, described pump chamber sealant
40 deform upon under external force, make the liquid in microchannel 103 accelerate flowing, it is achieved to microchannel
The conveying of liquid and flow control in 103.The second structure is: described fluid reservoir holds concurrently pump chamber 101 for elasticity
Material is made, and described pump chamber sealant 40 is rigid material layer.The pump chamber sealant 40 that elastomeric material is made
It is positioned on the second liquid storage sealant.So design pump chamber 101 of fluid reservoir can being held concurrently and carry out secondary seal.Also
Fluid reservoir pump chamber 101 of holding concurrently can be made to deform upon under external force, extruding fluid reservoir is held concurrently in pump chamber 101
Liquid so that it is flow into microchannel；Or, there is shape under external force in described fluid reservoir pump chamber 101 of holding concurrently
Become, make liquid in microchannel 103 accelerate flowing, it is achieved to the conveying of liquid in microchannel 103 and flow velocity
Control.
In this example, for the detection of solution in reative cell 105 on complex reaction room and microchannel layers 10,
Described micro-channels layer 20 offers the opening 201 corresponding with described reative cell 105, meanwhile,
Described detection layers 30 is provided with the carrying detection core for reactant liquor in reative cell 105 carries out biochemistry detection
Sheet or the detection zone of detection agent.Reative cell 105 on described reative cell and microchannel layers 10, microchannel
In the case of opening 201 on sealant 20 and the alignment of the detection zone in detection layers 30 compress, solid successively
Determine reative cell and microchannel layers 10, micro-channels layer 20 and detection layers 30, form one with reagent
The test card of the sealing of import, fluid passage and reative cell, further ensures test and blocks each working region
Sealing and security.
In this example, as it is shown on figure 3, described fluid reservoir is held concurrently, pump chamber 101 with reative cell and microchannel layers 10 is
Integrative-structure.As shown in Figure 4, in this programme, described detection layers 30 can be to be provided with magnetosensitive chip 302
Printed circuit board (PCB) 303.Magnetosensitive chip 302 can be replaced with by this test card according to actually detected needs
For detecting the biochemical sensor of other characteristics, and by wire, data are exported to external equipment.
Embodiment 2
As shown in Figure 6, the invention discloses a kind of microfluid test card, this test card includes reative cell and micro-
Channel layer 50, micro-channels layer and detection layers；This microfluid test card farther includes: externally protrude out in anti-
At least one fluid reservoir answering room and microchannel layers 50 is held concurrently pump chamber 60；Hold concurrently pump chamber 60 for sealing fluid reservoir
And isolate the first fluid reservoir sealant of the microchannel that can connect；It is positioned at described first fluid reservoir sealant relative
Hold concurrently the second liquid storage sealant of pump chamber 60 for sealing fluid reservoir in side；Waste liquid pool 501, externally protrudes out in reative cell
And microchannel layers 50 connecting with reative cell；And, the pump chamber being positioned at outside described second liquid storage sealant is close
Sealing.Described microchannel and reative cell are positioned at reative cell and the same side of microchannel layers 50, described fluid reservoir
Double pump chamber 60 and waste liquid pool 501 externally protrude out in the reative cell with reative cell opposite side and microchannel layers 50.
In this example, described first fluid reservoir sealant and the second fluid reservoir sealant are can be under external force
The film ruptured；The material of described second fluid reservoir sealant includes aluminum film layer, it is preferable that described first
The material of fluid reservoir sealant includes aluminum film layer.The material of described second fluid reservoir sealant farther includes
The etch resistant material layer with described pump chamber sealant opposite side on described aluminum film layer surface, it is preferred that described
The material of the first fluid reservoir sealant farther includes the same with described pump chamber sealant of described aluminium film surface
The etch resistant material layer of side.In this programme, described first fluid reservoir sealant and the second liquid storage sealant are close
Envelope fluid reservoir hold concurrently pump chamber 60 time, the etch resistant material layer of two fluid reservoir sealants is held concurrently pump chamber towards fluid reservoir
60, now, the liquid allowing fluid reservoir hold concurrently in pump chamber 60 contacts with resistant material, prevents liquid corrosion aluminium
Film layer.The material of this resistant to liquid corrosion can be polypropylene, polystyrene or be similarly used for protecting aluminium
The material of film not liquid body corrosion.
The pump chamber 60 of holding concurrently of fluid reservoir described in this example can be two kinds of structures.The first structure is: described storage
Liquid chamber pump chamber 60 of holding concurrently is made for rigid material, and described pump chamber sealant is elastomeric layer.Elastomeric material system
The pump chamber sealant become, is positioned on the second liquid storage sealant.So design pump chamber 60 of fluid reservoir can being held concurrently
Carry out secondary seal.Pump chamber sealant can also be made to deform upon under external force, extruding fluid reservoir is held concurrently
Liquid in pump chamber 60 so that it is flow into microchannel；Or, described pump chamber sealant is sent out under external force
Raw deformation, makes the liquid in microchannel accelerate flowing, it is achieved to the conveying of liquid in microchannel and flow velocity control
System.The second structure is: described fluid reservoir pump chamber 60 of holding concurrently is made for elastomeric material, described pump chamber sealant
For rigid material layer.The pump chamber sealant that elastomeric material is made, is positioned on the second liquid storage sealant.So set
Meter pump chamber 60 of can holding concurrently fluid reservoir carries out secondary seal.Fluid reservoir can also be made to hold concurrently under external force
Pump chamber 60 deforms upon, and extruding fluid reservoir is held concurrently the liquid in pump chamber so that it is flow into microchannel；Or, institute
State fluid reservoir pump chamber of holding concurrently to deform upon under external force, make the liquid in microchannel accelerate flowing, it is achieved
To the conveying of liquid in microchannel and flow control.
In this example, for the detection of solution in reative cell on complex reaction room and microchannel layers 50, in institute
State and on micro-channels layer, offer the opening corresponding with described reative cell, described detection layers sets meanwhile
Have and carry the detection zone of detection chip or detection agent for reactant liquor in reative cell is carried out biochemistry detection.?
Reative cell on described reative cell and microchannel layers, the opening on micro-channels layer and the inspection in detection layers
In the case of surveying district's alignment compression, fix reative cell and microchannel layers, micro-channels layer and detection successively
Layer, formed one with the test card of the sealing of reagent entry port, fluid passage and reative cell, protect further
Demonstrate,prove test and block sealing and the security of each working region.
In this example, as shown in Figure 6, described fluid reservoir pump chamber 60 of holding concurrently with reative cell and microchannel layers 50 is
Separable assembled structure, utilizes this structure user can to choose different materials make according to the character of selection liquid storage
Fluid reservoir hold concurrently pump chamber, with the storage demand of satisfied different liquid storages.
In this programme, described detection layers can be the printed circuit board (PCB) being provided with magnetosensitive chip.This test card is permissible
According to actually detected needs, magnetosensitive chip is replaced with the biochemical sensor for detecting other characteristics, and leads to
Cross wire to export data to external equipment.Described detection layers can also be provided with for reactant liquor in reative cell
Carry out the Fluorescent detector of biochemistry detection.When selecting to carry out fluoroscopic examination, need to select transparent inorganic material
Material makes detection layers or reative cell and microchannel layers, so that detection light can allow outside through test card
Equipment Inspection changes to fluoroscopic examination.
Embodiment 3
As it is shown in fig. 7, the invention discloses a kind of microfluid test card, this test card includes reative cell and micro-
Channel layer 70, micro-channels layer 80 and detection layers 90；This microfluid test card farther includes: outer
Convex at least one fluid reservoir in reative cell and microchannel layers 70 is held concurrently pump chamber 701；Hold concurrently for sealing fluid reservoir
First fluid reservoir sealant of the microchannel that pump chamber 701 and isolation can connect；It is positioned at described first fluid reservoir
Sealant opposite side is held concurrently the second liquid storage sealant of pump chamber 701 for sealing fluid reservoir；Waste liquid pool, externally protrudes out in
Reative cell and microchannel layers 70 also connect with reative cell；And, it is positioned at outside described second liquid storage sealant
Pump chamber sealant 85.Described microchannel and reative cell are positioned at reative cell and the same side of microchannel layers 70, institute
State fluid reservoir and hold concurrently pump chamber 701 and waste liquid pool externally protrudes out in the reative cell with reative cell opposite side and microchannel layers
On 70.
In this example, described first fluid reservoir sealant and the second fluid reservoir sealant are can be under external force
The film ruptured；The material of described second fluid reservoir sealant includes aluminum film layer, it is preferable that described first
The material of fluid reservoir sealant includes aluminum film layer.The material of described second fluid reservoir sealant farther includes
The etch resistant material layer with described pump chamber sealant 85 opposite side on described aluminum film layer surface, it is preferred that
The material of described first fluid reservoir sealant farther includes the close with described pump chamber of described aluminum film layer surface
The etch resistant material layer of sealing 85 homonymy.In this programme, described first fluid reservoir sealant and the second liquid storage
Sealant is when sealing fluid reservoir holds concurrently pump chamber 701, and the etch resistant material layer of two fluid reservoir sealants is towards storage
Liquid chamber is held concurrently pump chamber 701, and now, the liquid allowing fluid reservoir hold concurrently in pump chamber 701 contacts with resistant material, anti-
Only liquid corrosion aluminum film layer.The material of this resistant to liquid corrosion can be polypropylene, polystyrene or similar
For protecting the material that aluminium film not liquid body corrodes.
The pump chamber 701 of holding concurrently of fluid reservoir described in this example can be two kinds of structures.The first structure is: described storage
Liquid chamber pump chamber 701 of holding concurrently is made for rigid material, and described pump chamber sealant 85 is elastomeric layer.Elastic material
The pump chamber sealant 85 that material is made is positioned on the second liquid storage sealant.So design pump of fluid reservoir can being held concurrently
Room 701 carries out secondary seal.Pump chamber sealant 85 can also be made to deform upon under external force, squeeze
Pressure fluid reservoir is held concurrently the liquid in pump chamber 701 so that it is flow into microchannel；Or, described pump chamber sealant 85
Deform upon under external force, make the liquid in microchannel accelerate flowing, it is achieved to liquid in microchannel
Conveying and flow control.The second structure is: described fluid reservoir pump chamber 70 of holding concurrently is made for elastomeric material,
Described pump chamber sealant 85 is rigid material layer.The pump chamber sealant 85 that elastomeric material is made is positioned at the second storage
On liquid-tight sealing.So design pump chamber 701 of fluid reservoir can being held concurrently and carry out secondary seal.Can also be in external force
Under effect, making fluid reservoir pump chamber 701 of holding concurrently deform upon, extruding fluid reservoir is held concurrently the liquid in pump chamber 701, makes
It flows into microchannel；Or, described fluid reservoir pump chamber 701 of holding concurrently deforms upon under external force, makes miniflow
Liquid in passage accelerates flowing, it is achieved to the conveying of liquid in microchannel and flow control.
In this example, for the detection of solution in reative cell on complex reaction room and microchannel layers 70, in institute
State and on micro-channels layer, offer the opening corresponding with described reative cell, described detection layers sets meanwhile
There is the detection zone for the detection agent that reactant liquor in reative cell is carried out biochemistry detection.At described reative cell and micro-
Reative cell on channel layer 70, the opening on micro-channels layer and the alignment of the detection zone in detection layers compress
In the case of, fix reative cell and microchannel layers 70, micro-channels layer 80 and detection layers 90 successively,
Formed one with the test card of the sealing of reagent entry port, fluid passage and reative cell, further ensure
Test blocks sealing and the security of each working region.
In this example, described detection layers 90 is coated with for reactant liquor in reative cell is carried out biochemistry detection
Fluorescent detector.When selecting to carry out fluoroscopic examination, need to select transparent inorganic material to make detection layers
90 or reative cell and microchannel layers 70, so that detection light can allow external equipment detect through test card
Fluoroscopic examination changes.
In this example, described fluid reservoir pump chamber of holding concurrently can be structure as a whole with reative cell and microchannel layers, it is possible to
Being to be sealed with the fluid reservoir of the first and second fluid reservoir sealants pump chamber of holding concurrently to seal and be assembled in described reative cell
And the separable assembled structure in microchannel layers.In this programme, can be according to the needs of user's liquid storage, by described
Fluid reservoir holds concurrently sidewall and the reative cell of pump chamber and microchannel layers uses identical or different material to make.
Obviously, the above embodiment of the present invention is only for clearly demonstrating example of the present invention, and also
Non-is the restriction to embodiments of the present invention, for those of ordinary skill in the field, above-mentioned
Can also make other changes in different forms on the basis of explanation, here cannot be to all of enforcement
Mode gives exhaustive, every belongs to the obvious change or variation that technical scheme extended out
Row still in protection scope of the present invention.
Claims (12)
1. a microfluid test card, this test card includes reative cell and microchannel layers, micro-channels layer
And detection layers,
It is characterized in that, this microfluid test card farther includes:
Externally protrude out at least one fluid reservoir of reative cell and microchannel layers to hold concurrently pump chamber；
For sealing the first fluid reservoir sealant of microchannel that fluid reservoir holds concurrently pump chamber and isolation can connect；
Be positioned at described first fluid reservoir sealant opposite side for seal fluid reservoir hold concurrently pump chamber second liquid storage seal
Layer；
Waste liquid pool, externally protrudes out in reative cell and microchannel layers and connects with reative cell；And,
It is positioned at the pump chamber sealant outside described second liquid storage sealant.
Microfluid the most according to claim 1 test card, it is characterised in that described second fluid reservoir is close
The material of sealing includes aluminum film layer, it is preferable that the material of described first fluid reservoir sealant includes aluminium film
Layer.
Microfluid the most according to claim 2 test card, it is characterised in that described second fluid reservoir is close
The material of sealing farther includes described aluminum film layer surface with described pump chamber sealant opposite side corrosion resistant
Corrosion material layer, it is preferred that the material of described first fluid reservoir sealant farther includes described aluminum film layer table
The etch resistant material layer with described pump chamber sealant homonymy in face.
Microfluid the most according to claim 1 test card, it is characterised in that described fluid reservoir is held concurrently pump chamber
Sidewall be rigid material, described pump chamber sealant is elastomeric layer.
Microfluid the most according to claim 1 test card, it is characterised in that described fluid reservoir is held concurrently pump chamber
Sidewall elastomeric material, described pump chamber sealant is rigid material layer.
Microfluid the most according to claim 1 test card, it is characterised in that described fluid reservoir is held concurrently pump chamber
Sidewall be structure as a whole with reative cell and microchannel layers.
Microfluid the most according to claim 1 test card, it is characterised in that be sealed with first and second
The fluid reservoir of fluid reservoir sealant hold concurrently pump chamber seal be assembled in described reative cell and microchannel layers.
8. test card according to the microfluid described in claim 1 any one, it is characterised in that described liquid storage
Sidewall and the reative cell of pump chamber are held concurrently in room and microchannel layers uses identical or different making material.
Microfluid the most according to claim 1 test card, it is characterised in that described detection layers is for being provided with
The printed circuit board (PCB) of magnetosensitive chip.
10. test card according to the microfluid described in claim 1 any one, it is characterised in that described inspection
Surveying layer is to be provided with the Fluorescent detector for reactant liquor in reative cell carries out biochemistry detection.
11. microfluid according to claim 10 test cards, it is characterised in that described detection layers or anti-
Answering room and microchannel layers is transparent material.
12. microfluid according to claim 1 test cards, it is characterised in that
The opening corresponding with described reative cell is offered on described micro-channels layer；
Described detection layers be provided with the carrying detection chip for reactant liquor in reative cell being carried out biochemistry detection or
The detection zone of detection agent；
In reative cell on described reative cell and microchannel layers, the opening on micro-channels layer and detection layers
Detection zone alignment compress in the case of, fix successively reative cell and microchannel layers, micro-channels layer and
Detection layers, forms a test card with the sealing of reagent entry port, fluid passage and reative cell.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610225192.5A CN105866450A (en) | 2016-04-12 | 2016-04-12 | Micro-fluid test card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610225192.5A CN105866450A (en) | 2016-04-12 | 2016-04-12 | Micro-fluid test card |
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| Publication Number | Publication Date |
|---|---|
| CN105866450A true CN105866450A (en) | 2016-08-17 |
Family
ID=56636582
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610225192.5A Pending CN105866450A (en) | 2016-04-12 | 2016-04-12 | Micro-fluid test card |
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| CN108120755A (en) * | 2017-12-20 | 2018-06-05 | 深圳市国赛生物技术有限公司 | Detection device and its application |
| CN113275045A (en) * | 2020-02-20 | 2021-08-20 | 北京京东方健康科技有限公司 | Detection chip, operation method thereof and detection system |
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| WO2021164529A1 (en) * | 2020-02-20 | 2021-08-26 | 京东方科技集团股份有限公司 | Detection chip, operation method therefor and detection system |
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Application publication date: 20160817 |