CN105858913B - A method of utilizing the biological reinforced processing pulp-making waste-water of Phanerochaete chrysosporium - Google Patents
A method of utilizing the biological reinforced processing pulp-making waste-water of Phanerochaete chrysosporium Download PDFInfo
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- CN105858913B CN105858913B CN201610320726.2A CN201610320726A CN105858913B CN 105858913 B CN105858913 B CN 105858913B CN 201610320726 A CN201610320726 A CN 201610320726A CN 105858913 B CN105858913 B CN 105858913B
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/26—Nature of the water, waste water, sewage or sludge to be treated from the processing of plants or parts thereof
- C02F2103/28—Nature of the water, waste water, sewage or sludge to be treated from the processing of plants or parts thereof from the paper or cellulose industry
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Abstract
The invention discloses a kind of method using the biological reinforced processing pulp-making waste-water of Phanerochaete chrysosporium, step includes：1）The domestication of activated sludge and biofilm；2）It is prepared by Phanerochaete chrysosporium bacterium solution；3）Biological reinforced processing waste water.The method using the biological reinforced processing pulp-making waste-water of Phanerochaete chrysosporium of the present invention, utilize the synergistic effect of Phanerochaete chrysosporium and biomembrane indigenous microorganism, a degree of biodegradation is carried out to lignin, the small molecule of generation can be utilized and degraded by biofilm microorganisms, therefore the biological reinforcing method is more preferable to the treatment effect of pulp-making waste-water.It is tested compared with by being carried out with control, in 300 mg/L of glucose content, pH 6.0,0.2 mg/L of ammonium tartrate content, 100 mL/L of whiterot fungi bacteria suspension dosage, 26 DEG C of temperature, under the conditions of 8 mg/L of DO, COD removal rates and chroma removal rate respectively reach 89.74%, 66.67%, and it is 37.76mg/L that COD is discharged after processing, coloration is 20, complies fully with discharging standards.
Description
Technical field
The invention belongs to industrial waste water treatments, and in particular to a kind of to utilize Phanerochaete chrysosporium
（P.Chrysosporium）The method of biological reinforced processing pulp-making waste-water.
Background technology
Pulp and paper industry is one of six big contaminated industries in the world, and a large amount of lignin, cellulose are contained in waste water
With various chemicals, oxygen demand is big, is one of the main source for polluting environment.Paper industry annual waste discharge amount in China's reaches
4000000000 tons, 1/6, the COD and SS for accounting for national discharged volume of industrial waste water account for 1/4, have that discharge capacity is big, pollutant is multiple on the whole
The features such as miscellaneous, difficult, the development of paper industry is not only hindered, has also seriously affected daily life and the natural environment of the common people
Improvement.The emission limit for the pulp and paper industry wastewater discharge standard that China in 2008 enacts and enforces is more tighter than old plant
Lattice.According to GB3544-2008, national regulation slurrying enterprise wastewater COD emission limits are 100 mg/L, slurrying and papermaking joint life
It is 90 mg/L to produce enterprise's COD emission limits, and papermaking enterprise is 80 mg/L, BOD5Emission limit is 20 mg/L.Therefore, slurrying
Papermaking enterprise must seek effective new process for treating waste water, can be only achieved the emission request of new standard, to promoting ecology
Environmental protection and the sustainable development of paper industry have important realistic meaning.
Paper-making effluent pollutant concentration is high, and complicated component, biodegradability is poor, and flow and load fluctuation are larger, become
One of intractable industrial wastewater.Its processing method mainly has Physical, chemical method, physico-chemical process and bioanalysis.Physical includes
Bubble-floating Method, ultrasonic method etc., chemical method include chemical oxidization method, photoelectrocatalysis method etc., and physical-chemical process includes membrane separation process, coagulation
Method, absorption method etc., but either physical treatment process or method of chemical treatment, can all cause environment secondary pollution, and of high cost,
It is unsuitable for commonly used.Wherein biological method is at low cost, efficient, will not cause secondary pollution to environment, is current mainstream
Wastewater treatment method.But in recent years due to the rapid development of social economy, wastewater flow rate is increasing, and ingredient is increasingly complicated, and one
As the processing capacity of bioanalysis be difficult to meet actual demand, especially when containing temporary toxic difficult substance in waste water,
Traditional treatment process is even more to be difficult to reach ideal effluent quality, and to the balance of original system and stabilized zone can also come very
Big risk.Biological reinforcing technology is produced in this context, which refers to being added with specific in biological treatment system
Microorganism, nutrients or the matrix analog of function, specific pollutants of degrading, improve degradation rate, are effectively treated to reach
The purpose of waste water containing hardly degraded organic substance.
The main reason for paper-making effluent high COD high chromas is wherein to contain the objects difficult to degrade such as a large amount of lignocellulosic
Matter.Lignin belongs to high molecular polymer, has amorphism and tridimensional network, is that microorganism generally acknowledged at present is difficult to degrade
One of aromatic compound.Paper-making effluent is handled, discharging standards is reached, is that must handle difficulty after all
The lignin of degradation.It is that fungi, bacterium and corresponding microorganism group are coefficient as a result, wherein true that lignin, which wants degradable,
Bacterium plays a leading role in lignin degradation, in all fungal species whiterot fungi be currently known in nature to wooden
Element possess most strong degradation capability a kind of fungi and it is known uniquely can be by the final mineralising of lignin under the conditions of pure culture
Microorganism, therefore, whiterot fungi often become research lignin degrading preferred microorganism.Handle the research of paper-making effluent
Show that whiterot fungi has apparent decoloration to Pulp Bleaching Wastewater, eliminates toxicity, reduce the effect of COD and BOD.Wang Shuanfei etc.
People's autonomous Design downflow type fixed bed reactors, using absorption method fixed white rot bacterium, the mixing of processing reed pulp CEH tri-stage bleachings is useless
Water, test continuous operation the result shows that, absorption method fixed white rot bacterium can promote treatment effect relatively to stablize, and COD removal rates are
65%-68%, BOD5Removal rate is 89%-92%.Xu sea is beautiful et al. to use activated carbon-chemical fibre fixed-film reactor, adds white
Rotten bacterium handles the composite waste of reed pulp CEH tri-stage bleachings, the study found that the percent of decolourization of bleaching effluent reaches 60%-90%, COD removals
Rate is 50% or more.Yan Bing et al. whiterot fungi be applied to CTMP pulping waste liquors processing in, result of study show whiterot fungi in addition to
It can well adapt to CTMP waste liquids, moreover it is possible to which pollutant therein of significantly degrading, the removal rate of waste water COD reach 68%-73%.Slurrying
Problem is decoloration there are one in paper waste, and biological reinforcing technology is applied to also obtain certain progress in decolorization.Zeng Li
Fine jade et al. handles dyeing waste water using biological reinforcing technology using dominant bacteria, and specific practice is thrown in the hydrolytic tank of dyeing waste water
Enter the hybrid bacterial strain of pseudomonad with preferable decolorizing effect, Aeromonas and red spirillum, as a result display continues to handle for six days
System percent of decolourization is stable in 90% or more.
Invention content
Goal of the invention：For the deficiencies in the prior art, yellow archespore hair is utilized the object of the present invention is to provide a kind of
The method of the flat biological reinforced processing pulp-making waste-water of lead fungi carries out biological reinforced place using Phanerochaete chrysosporium to pulp-making waste-water
Reason improves COD removal rates, while effectively reducing waste water color, removes coloration.
Technical solution：In order to achieve the above-mentioned object of the invention, the technical solution adopted by the present invention is：
A method of using the biological reinforced processing pulp-making waste-water of Phanerochaete chrysosporium, steps are as follows：
1）Bioreactor is taken, industrial grouping filler and activated sludge is added, and it is carbon source that glucose, which is added, is exposed
Gas stops aeration certain time, water is changed with the mode of siphon supernatant daily, after a period of time is aerated, sludge success biofilm；
2）By Phanerochaete chrysosporium from being inoculated on inclined-plane in potato fluid nutrient medium, bacteria suspension, constant temperature 37 is made
DEG C in shaking table culture three to four days；
3）Phanerochaete chrysosporium bacteria suspension is added in the bioreactor, continues to be aerated, and change water daily；Put into bacterium
After agent couple of days, supernatant is sucked out, pulp-making waste-water is added, while being 200 by C, N, P:5:1 mass ratio is separately added into tartaric acid
Ammonium and KH2PO4, and macroelement and trace element that Institute of Micro-biology needs is added, change waste water daily later, what measurement was discharged daily
COD and coloration, water quality meet emission request discharge.
Step 1）In, activated sludge concentration 20g/L, concentration of glucose 500mg/L stop aeration one hour daily.
Step 3）In, in waste water, the alkaline lignin of glucose and 300mg/L containing 200mg/L.
Step 3）In, 18 ~ 40 DEG C of temperature, pH4.5 ~ 9.0,0 ~ 300 mg/L of glucose content, ammonium tartrate content 0 ~
0.4mg/L, Phanerochaete chrysosporium 10 ~ 200mL/L of bacteria suspension dosage and 2 ~ 8mg/L of dissolved oxygen.
Step 3）In, 18 DEG C of temperature, pH 7.0,300 mg/L of glucose content, 0.02 mg/L of ammonium tartrate content, Huang
Archespore hair flat 100 mL/L of lead fungi bacteria suspension dosage and 4 mg/L of dissolved oxygen.
Step 3）In, glucose content 300mg/L, pH 6.0, ammonium tartrate content is 0.2 mg/L, yellow archespore hair
Flat lead fungi dosage is 100 mL/L, and temperature is 26 DEG C, and DO is 8 mg/L.
Advantageous effect：Compared with prior art, the utilization biological reinforced processing slurrying of Phanerochaete chrysosporium of the invention is useless
The method of water carries out to a certain degree lignin using the synergistic effect of Phanerochaete chrysosporium and biomembrane indigenous microorganism
Biodegradation, the small molecule of generation can utilize and degraded by biofilm microorganisms, therefore the biological reinforcing method is useless to slurrying
The treatment effect of water is more preferable.It is tested compared with by being carried out with control, in 300 mg/L of glucose content, pH 6.0, ammonium tartrate
0.2 mg/L of content, 100 mL/L of whiterot fungi bacteria suspension dosage, 26 DEG C of temperature, under the conditions of 8 mg/L of DO, COD removal rates
89.74%, 66.67% is respectively reached with chroma removal rate, it is 37.76mg/L that COD is discharged after processing, and coloration 20 complies fully with
Discharging standards have good practicability, can generate good economic benefit and social effect.
Description of the drawings
Fig. 1 is the COD and chromatic value result figure of biological reinforced processing；
Fig. 2 is the COD removal rates and chroma removal rate result figure of waste water；
Fig. 3 is content of lignin result of variations figure in waste water.
Specific implementation mode
With reference to specific embodiment, the present invention is described further.Raw materials and reagents used in following embodiment
Specially：Phanerochaete chrysosporium（P.Chrysosporium）：It is purchased from Microbe Inst., Chinese Academy of Sciences；Alkaline lignin
（Kraft lignin）：It is purchased from Sigma-Aldrich companies；Activated sludge：It is heavy to be derived from Nanjing middle bar sewage treatment plant two
Pond；Industrial grouping filler：It is purchased from Wuxi Yixing environmental protection company.
Biofilm culture medium prescription：Glucose 500mg, distilled water 1000mL.
Paper waste ingredient：Glucose 200mg, alkaline lignin 300mg, ammonium tartrate 0.2g, benzyl alcohol 0.5g,
MgSO4•H2O 0.71g, KH2PO42.56g, VB1 0.001g, Tween 80 1.0g, micro- 70mL, distilled water
1000mL。
Trace element solution（g/L）：Amion acetic acid 0.6；MnSO4•H2O 0.5；NaCl 1；FeSO4•7H2O 0.1；
CoSO4•7H2O 0.22；CaCl2•2H2O 1.56；ZnSO4•7H2O 0.1；CuSO4•5H2O 0.1；AlK(SO4)2•12H2O
0.01；HBO30.01；Na2MoO4•2H2O 0.01。
Potato culture：Potato 200g；Sucrose（Or glucose）20g；Peeling potatoes are cut into block and boil 30min,
Then filtered through gauze, then sugaring and agar are used, distilled water is supplemented after dissolving to 1000 mL.121 DEG C of sterilizing 30min.
Embodiment 1
A method of using the biological reinforced processing pulp-making waste-water of Phanerochaete chrysosporium, steps are as follows：
1）The domestication of activated sludge and biofilm：It takes the beaker of several 2000 mL that bioreactor is made, is added wherein
Industrial grouping filler and activated sludge, activated sludge concentration are about 20g/L, and the glucose that 500mg/L is added is carbon source, into
Row aeration, stops aeration one hour, with siphon method Aspirate supernatant, is supplemented the nutritive water with supernatant same volume daily
（500mg/L glucose solutions）, after a period of time is aerated, sludge success biofilm.
2）Prepare bacterium solution：Phanerochaete chrysosporium is inoculated into the potato Liquid Culture of 250mL conical flasks from inclined-plane
In base, it is made bacteria suspension, 37 DEG C of constant temperature was in shaking table culture three to four days.
3）Biological reinforced processing：Phanerochaete chrysosporium bacteria suspension is added in the bioreactor, separately takes reactor conduct
Control, does not add Phanerochaete chrysosporium.Continue to be aerated, and changes nutritive water daily.After putting into microbial inoculum about couple of days, it is sucked out
Nutritive water is changed to pulp-making waste-water by supernatant（Contain 200mg/L glucose and 300mg/L alkalinity lignin）, while by C,
N, P is 200:5:1 mass ratio is separately added into ammonium tartrate and KH2PO4, and the macroelement and micro that Institute of Micro-biology needs is added
Element.Change water daily later, the composition of water is constant, measures the COD being discharged daily and coloration, and the continuous data for surveying two weeks carry out
Comparison.
The bacteria suspension of Phanerochaete chrysosporium is added to using glucose and Kraft lignin in the waste water of carbon source, to connect
Continuous observation 15 days, measures 9 groups of COD and chromatic value, as shown in Figure 1, it is clear that, control group is discharged COD and is nearly all more than 100 mg/
L, the reactor water outlet COD for adding Phanerochaete chrysosporium is nearly all less than 100 mg/L, and at the initial stage that adds and adds 10 days
COD drops to 50mg/L hereinafter, having larger difference with control group later, it is seen that Phanerochaete chrysosporium has pulp-making waste-water bright
The effect of aobvious decoloration, reduction COD.
The COD removal rates and chroma removal rate for adding biological reinforced experiment process waste water are calculated with Fig. 1 the data obtaineds, are tied
Fruit is as shown in Figure 2.It can be seen that reaching maximum in third day to the 7th day this stage COD removal rate, between 60%-70%, arrive
COD removal rates rise again at the 13rd day, reach 52.03%.And reach maximum in the 13rd day chroma removal rate, it is 37.5%.Processing
Initial stage COD removal rate is higher and chroma removal rate is relatively low, after processing reaction fortnight, Phanerochaete chrysosporium obvious degradation mould
Lignin in quasi- paper waste, while so that coloration is reduced and declining with COD.
Embodiment 2
It is same as Example 1, wherein in life using the method for the biological reinforced processing pulp-making waste-water of Phanerochaete chrysosporium
In object intensive treatment step, technological parameter is 18 DEG C of temperature, pH 7.0,300 mg/L of glucose content, ammonium tartrate content
0.02 mg/L, 100 mL/L of Phanerochaete chrysosporium bacteria suspension dosage and 4 mg/L of dissolved oxygen, successive reaction are surveyed after seven days
COD and coloration, under the conditions of this, COD removal rates 71.36%, chroma removal rate 75%.
Embodiment 3
It is same as Example 1, wherein in life using the method for the biological reinforced processing pulp-making waste-water of Phanerochaete chrysosporium
In object intensive treatment step, glucose content 300mg/L, pH 6.0, ammonium tartrate content is 0.2 mg/L, yellow archespore hair
Flat lead fungi dosage is 100 mL/L, and temperature is 26 DEG C, and DO is 8 mg/L, and successive reaction surveys COD and coloration after seven days, at this time
COD removal rates and chroma removal rate respectively reach 89.74%, 66.67%, i.e. original waste water COD is 368.12 mg/L, and coloration is
60, it is 37.76mg/L that COD is discharged after processing, and coloration 20 complies fully with discharging standards.At this time to the removal ability of COD
10% or so are improved than prior art efficiency.
The content of lignin being discharged daily is measured simultaneously, it can be seen that, after operation in 24 hours, lignin contains by Fig. 3
Amount by 200mg/L is reduced to 0mg/L, illustrates that lignin is completely degraded in biological reinforced processing as micromolecular compound, to
Water outlet COD and coloration are reduced, verifies Phanerochaete chrysosporium again as the biological reinforced processing pulp-making waste-water of function bacterium, quick height
Effect reaches Chinese effluent standard.
Claims (6)
1. a kind of method using the biological reinforced processing pulp-making waste-water of Phanerochaete chrysosporium, which is characterized in that steps are as follows：
1) bioreactor is taken, industrial grouping filler and activated sludge is added, and it is carbon source that glucose, which is added, is aerated, often
It stops aeration certain time, and water is changed with the mode of siphon supernatant, after a period of time is aerated, sludge success biofilm；
2) by Phanerochaete chrysosporium from being inoculated on inclined-plane in potato fluid nutrient medium, it is made bacteria suspension, 37 DEG C of constant temperature exists
Shaking table culture three to four days；
3) Phanerochaete chrysosporium bacteria suspension is added in the bioreactor, continues to be aerated, and change water daily；Put into microbial inoculum two
After three days, supernatant is sucked out, pulp-making waste-water is added, while being 200 by C, N, P:5:1 mass ratio be separately added into ammonium tartrate and
KH2PO4, and macroelement and trace element that Institute of Micro-biology needs is added, changes waste water daily later, measure the COD being discharged daily and
Coloration, water quality meet emission request discharge.
2. the method according to claim 1 using the biological reinforced processing pulp-making waste-water of Phanerochaete chrysosporium, feature
It is, in step 1), activated sludge concentration 20g/L, concentration of glucose 500mg/L stop aeration one hour daily.
3. the method according to claim 1 using the biological reinforced processing pulp-making waste-water of Phanerochaete chrysosporium, feature
It is, in step 3), in waste water, the alkaline lignin of glucose and 300mg/L containing 200mg/L.
4. the method according to claim 1 using the biological reinforced processing pulp-making waste-water of Phanerochaete chrysosporium, feature
Be, in step 3), 18~40 DEG C of temperature, pH4.5~9.0,0~300mg/L of glucose content, ammonium tartrate content 0~
0.4mg/L, Phanerochaete chrysosporium 10~200mL/L of bacteria suspension dosage and 2~8mg/L of dissolved oxygen.
5. the method according to claim 1 using the biological reinforced processing pulp-making waste-water of Phanerochaete chrysosporium, feature
It is, in step 3), 18 DEG C of temperature, pH 7.0, glucose content 300mg/L, ammonium tartrate content 0.02mg/L, yellow archespore hair
Flat lead fungi bacteria suspension dosage 100mL/L and dissolved oxygen 4mg/L.
6. the method according to claim 1 using the biological reinforced processing pulp-making waste-water of Phanerochaete chrysosporium, feature
It is, in step 3), glucose content 300mg/L, pH 6.0, ammonium tartrate content is 0.2mg/L, yellow archespore Mao Pingge
Bacterium bacteria suspension dosage is 100mL/L, and temperature is 26 DEG C, DO 8mg/L.
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| JP2005349327A (en) * | 2004-06-11 | 2005-12-22 | Univ Nagoya | Method for treating waste water |
| EP1679287A1 (en) * | 2005-01-05 | 2006-07-12 | Sorce, Inc. | Wastewater treatment method utilizing white rot and brown rot fungi |
| CN103663720A (en) * | 2013-09-10 | 2014-03-26 | 江苏大学 | Technique for removing gallic acid in wastewater by using Phanerochaete chrysosporium thallus |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005349327A (en) * | 2004-06-11 | 2005-12-22 | Univ Nagoya | Method for treating waste water |
| EP1679287A1 (en) * | 2005-01-05 | 2006-07-12 | Sorce, Inc. | Wastewater treatment method utilizing white rot and brown rot fungi |
| CN103663720A (en) * | 2013-09-10 | 2014-03-26 | 江苏大学 | Technique for removing gallic acid in wastewater by using Phanerochaete chrysosporium thallus |
Non-Patent Citations (1)
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| 白腐真菌-活性污泥联合处理棉浆黑液的研究;赵丽红等;《生态环境学报》;20090318;第18卷(第2期);第422-423页第1.1、1.2、1.3.1、1.3.2、1.3.3小节,第423页第1.3.4小节、第424页第2.4小节及图4 * |
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Application publication date: 20160817 Assignee: NANJING YUANYI ENVIRONMENT BIOLOGICAL ENGINEERING CO., LTD. Assignor: Nanjing Forestry University Contract record no.: X2019320000250 Denomination of invention: Method of using phanerochaete chrysosporium for bio-augmentation treatment of pulping wastewater Granted publication date: 20181023 License type: Common License Record date: 20191114 |