CN105851483B - Method for preparing feed based on traditional Chinese medicine dregs - Google Patents
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Abstract
The invention discloses a method for preparing feed based on traditional Chinese medicine dregs, which comprises the steps of carrying out explosion pretreatment on raw materials by a urea solvent, carrying out mixed fermentation by multiple strains, and finally carrying out crushing and granulation technology to obtain the traditional Chinese medicine dreg coarse feed. The feed provided by the invention has high crude protein content and rich nutrition, and is easy to be absorbed by animals. The method can avoid environmental pollution caused by the traditional Chinese medicine residues, and simultaneously relieve the problem of feed shortage in the development of animal breeding industry, and has wide application prospect.
Description
Technical Field
The invention belongs to the technical field of animal feed production, and relates to a method for preparing feed based on traditional Chinese medicine dregs.
Background
The residue of the Chinese medicinal materials is residue obtained after extraction with certain solvent or other methods. At present, the traditional Chinese medicine residues are mostly used as garbage, which not only wastes resources greatly, but also pollutes the environment. With the development of animal husbandry and breeding industry, traditional feed resources (various grain bran soybean meal and the like) cannot meet the rapidly-growing feed market, animal feed comprehensively fermented by traditional Chinese medicine dregs is adopted, after pigs, cattle and sheep eat the feed, protein, calcium, magnesium, iron and other trace elements are supplemented, part of drug effects in the dregs have certain prevention and cure effects on various diseases of livestock and poultry, the immunity is improved, the death rate is reduced, the utilization rate of the feed can be improved, the morbidity is reduced, and the quality of bred animals is improved. Therefore, the production of functional feed by using the Chinese medicine residue as a raw material is a new technology with development potential.
The steam explosion pretreatment technology is widely applied to the defibration of cellulose raw materials, but the steam explosion technology can only partially degrade the cellulose, and the subsequent treatment is still difficult. The urea solution can destroy hydrogen bonds in the cellulose, and the thoroughness of cellulose degradation is ensured by combining with high-temperature and high-pressure pretreatment conditions. Meanwhile, the urea is an ideal non-protein nitrogen supplement source for cattle, sheep and the like of ruminants, the total nitrogen of the urea is 46 percent, the nitrogen is converted into crude protein of 28.75 percent, namely the nitrogen content of 1kg of urea is equivalent to 2.6 to 2.8kg of crude protein or 7kg of bean cakes.
Therefore, the invention applies the urea solution blasting technology to the defibering of the traditional Chinese medicine residues containing more cellulose and the plant straws, which is an innovation. Meanwhile, the method combines the microbial degradation technology, adopts white-rot fungi mixed strains capable of secreting cellulolytic enzymes, adds yellow wine vinasse, and utilizes the original lactic acid bacteria and saccharomycetes in the vinasse to perform mixed fermentation of multiple strains. Not only can ensure that the raw materials are fully degraded, but also can use the vinasse containing rich amino acid, vitamin and protein for producing the feed, and ensure that the nutrient components can be directly absorbed and utilized by animals. And finally, by a crushing and granulating technology, the influence of the granularity on the absorptivity of the nutrient components is eliminated, and the maximum absorptivity is ensured. The method for producing the animal feed by comprehensively treating the Chinese medicine residues and the plant straws as the main raw materials has no patent protection and report.
Disclosure of Invention
The invention provides a method for preparing feed based on traditional Chinese medicine dregs, aiming at the problem of resource waste of plant straws, traditional Chinese medicine dregs and the like.
The purpose of the invention is realized by the following technical scheme: a method for preparing feed based on traditional Chinese medicine dregs comprises the following steps:
(1) respectively crushing 100-120 parts of traditional Chinese medicine dregs, 50-100 parts of straws and 20-40 parts of wheat bran into granules with the particle size of 0.4-0.8cm, and uniformly mixing to obtain a granular mixture.
(2) Dispersing the granular mixture into water with the same mass, and then adding urea, wherein the mass of the urea is 1-5% of the total mass of the granular mixture; turning over the materials once every half hour.
(3) And (3) heating the material obtained in the step (2) to 196-240 ℃ for steaming and explosion treatment, wherein the treatment time is 0.3-20 min, and the pressure is 1-3 MPa.
(4) And (3) cooling the material treated in the step (3), spraying 1-10 parts of fungus bacterial liquid, adding 10-20 parts of yellow wine vinasse, uniformly mixing, stacking and fermenting at 22-28 ℃ for 20-30 days, and turning the stack every 4-7 days.
(5) Drying the fermented material by a dryer until the water content is 15-20 wt%.
(6) Making the dried material into granules with particle size of 0.3-1.0mm to obtain the feed.
Further, the fungus liquid is prepared by the following method:
(1) preparing fungus seed liquid, namely taking white rotten wood, digging strain blocks, putting the strain blocks into a fresh potato glucose agar (PDA) culture medium, and performing shake culture at 25 ℃ for 60-72h at the rotation speed of 120 plus 150rpm to prepare the fungus seed liquid;
(2) the preparation of the fungus bacterial liquid comprises the steps of adding the fungus seed liquid into a PDA liquid culture medium according to the volume ratio of 0.3-0.8%, maintaining the temperature at 25 ℃, and culturing for 2-5 days under the culture condition of the rotation speed of 120-150rpm, thus obtaining the fungus bacterial liquid.
Further, the strain is Phanerochaete chrysosporium, Coriolus versicolor, tramete versicolor or Phlebia, preferably Phanerochaete chrysosporium.
Further, the traditional Chinese medicine dregs are mixed dregs composed of one or more of radix scutellariae dregs, radix isatidis dregs, rhizoma acori graminei dregs, angelica sinensis dregs and liquorice dregs according to any ratio.
Further, the medicine dregs are prepared from radix scutellariae, radix isatidis, rhizoma acori graminei, angelica sinensis and liquorice according to a mass ratio of 4-6: 6-8: 6-8: 6-8: 8 to 10.
Further, the pH value of the yellow wine vinasse is adjusted to 5-7 by baking soda, so that the activity of lactic acid bacteria and yeast in the vinasse is ensured.
The invention takes the waste plant straws and traditional Chinese medicine dregs as main components, not only solves the problem of environmental pollution, but also realizes the recycling of resources, obtains animal feed with rich nutrition and easy absorption, and has the following advantages:
firstly, the invention adopts the urea solution blasting technology, the urea solution is added into the feed to destroy hydrogen bonds between cellulose molecules, compared with the common steam blasting technology, the invention leads the fiber decomposition of the raw material to be more thorough, thereby being beneficial to the enzymolysis, and compared with the direct application of fungus fermentation, the invention greatly shortens the fermentation time. The added urea can be used as a non-protein nitrogen supplement source for animals.
Secondly, edible fungi are adopted for fermentation, a proper amount of vinasse is added, and lactic acid bacteria and saccharomycetes in the vinasse are utilized, so that the raw materials can be decomposed into components which can be directly absorbed by livestock, and the safety and the nutrition of the edible feed are guaranteed.
Thirdly, the dried and granulated feed is prepared into the cultivation feed with moderate water content and proper granularity, which is most suitable for livestock to eat, and the factors of the granularity influencing the absorptivity are eliminated.
Moreover, the technical scheme and the method provided by the scheme are simple, and the industrialization requirement can be greatly met.
Drawings
FIG. 1 is a graph showing the effect of different amounts of distiller's grains on the polysaccharide content after fermentation;
fig. 2 is a technical route diagram of the present invention.
Detailed Description
The following detailed description of one embodiment of the present invention is merely a preferred embodiment of the present invention and should not be taken as limiting the scope of the invention.
Example 1
(1) The preparation method of the fungus seed liquid comprises collecting white rotten wood, digging strain block, adding into fresh potato glucose agar (PDA) culture medium, shake culturing at 25 deg.C for 72 hr at 150rpm, and making into fungus seed liquid.
(2) The preparation method of fungus bacterial liquid comprises adding fungus seed liquid 0.3 vol% into PDA liquid culture medium, maintaining the culture conditions of 25 deg.C and 120rpm, and culturing for 5 days to obtain fungus bacterial liquid (OD600 of 0.8-1).
(3) Respectively crushing 100 parts of angelica sinensis residues, 50 parts of straws and 20 parts of wheat bran into particles with the particle size of 0.4cm, and uniformly mixing to obtain a granular mixture.
(4) The granular mixture was dispersed in an equal mass of water, then urea in the amount shown in table 1 was added and the impregnation was carried out for 4 hours, turning the batch once every half hour.
(5) And (4) heating the material obtained in the step (4) to 200 ℃ for steam explosion treatment, wherein the treatment time is 5min, and the pressure is 3 MPa.
(6) And (3) cooling the material treated in the step (5), spraying 8 parts of fungus liquid, adding 10 parts of yellow wine vinasse, uniformly mixing, stacking and fermenting for 30 days at the temperature of 22-28 ℃, and turning the stack every 4 days.
(7) And drying the fermented material by a dryer until the water content is 20 wt%.
(8) And (4) preparing the dried materials into granules with the grain size of 0.3mm to obtain the feed.
The obtained parameters of the prepared feed are shown in table 1 and table 2.
TABLE 1 different steam explosion methods for treating material composition changes
As can be seen from Table 1, the cellulose degradation rate is significantly improved after urea is added, and the urea solution has no significant influence on the degradation of hemicellulose and lignin.
TABLE 2 measurement of blood routine index of test cattle
(the unit is glutamic-pyruvic transaminase, Karman unit/L serum, blood sugar mg/L plasma and urea nitrogen mg/L serum. the three biochemical common values of the blood of the dairy cattle are 450-1300 mg/L plasma, 100-300 Karman unit/L serum and 60-270 mg/L serum of urea nitrogen respectively.)
As can be seen from Table 2, after the urea is added, the average values of the three indexes of the cattle are all in a normal range, which shows that the added urea has no toxic or side effect on the cattle and no abnormal influence on the kidney and liver functions, and the feed is safe and reliable.
Example 2 this example prepared materials using the same method and prepared feeds of different particle sizes as shown in the table below.
TABLE 3 Effect of different particle sizes on pellet feed quality
As can be seen from Table 3, as the pulverization particle size decreases, the pulverization rate of the pellet feed decreases, the hardness increases, and the stability in water increases.
Example 3 this example uses fungi in conjunction with yeast in distillers' grains for fermentation, and it is expected that the enzyme systems of each strain complement each other to increase the polysaccharide content.
(1) Respectively crushing 100 parts by weight of the traditional Chinese medicine dregs, 100 parts by weight of straws and 40 parts by weight of wheat bran into granules with the particle size of 0.8cm, and uniformly mixing to obtain a granular mixture.
(2) Dispersing the granular mixture into water with the same mass, and then adding urea, wherein the mass of the urea is 5% of the total mass of the granular mixture; dipping for 4 hours, and turning over the materials once every half an hour.
(3) And (3) heating the material obtained in the step (2) to 240 ℃ for steam explosion treatment, wherein the treatment time is 20min, and the pressure is 3 MPa.
(4) And (4) cooling the material treated in the step (3), spraying 5 parts by weight of fungus bacterial liquid, adding different parts by weight of yellow wine vinasse, uniformly mixing, stacking and fermenting at 22-28 ℃ for 28 days, and turning the stack every 4 days.
(5) Drying the fermented material by a dryer until the water content is 15-20 wt%.
(6) And (4) preparing the dried materials into particles with the particle size of 1.0mm to obtain the feed.
As shown in FIG. 1, it can be seen from the graph that the polysaccharide content is the highest when the amount of the pot ale added is 16 parts.
Although the invention has been described in detail hereinabove with respect to specific embodiments thereof, it will be apparent to those skilled in the art that many modifications and improvements can be made thereto based on the invention. Therefore, it is intended that all such modifications and improvements be included within the scope of the invention without departing from the spirit thereof.
Claims (5)
1. A method for preparing feed based on traditional Chinese medicine dregs is characterized by comprising the following steps:
(1) Respectively crushing 100-120 parts by weight of traditional Chinese medicine residues, 50-100 parts by weight of straws and 20-40 parts by weight of wheat bran into particles with the particle size of 0.4-0.8cm, and uniformly mixing to obtain a granular mixture;
(2) Dispersing the granular mixture into water with the same mass, and then adding urea, wherein the mass of the urea is 1-5% of the total mass of the granular mixture; dipping for 4 hours, and turning the materials once every half hour;
(3) Heating the material obtained in the step (2) to 196-240 ℃ for steaming and explosion treatment, wherein the treatment time is 0.3-20 min, and the pressure is 1-3 MPa;
(4) Cooling the material treated in the step (3), spraying 1-10 parts by weight of fungus bacterial liquid, adding 10-20 parts by weight of yellow wine vinasse, uniformly mixing, stacking and fermenting at 22-28 ℃ for 20-30 days, and turning the stack every 4-7 days;
the pH value of the yellow wine vinasse is adjusted to 5-7 by baking soda, so that the activity of lactic acid bacteria and yeast in the vinasse is ensured;
(5) drying the fermented material by a dryer until the water content is 15-20 wt%;
(6) making the dried material into granules with particle size of 0.3-1.0mm to obtain the feed.
2. The method according to claim 1, wherein the fungus liquid is prepared by the following method:
(1) Preparing fungus seed liquid, namely taking white rotten wood, digging strain blocks, putting the strain blocks into a fresh potato glucose agar (PDA) culture medium, and performing shake culture at 25 ℃ for 60-72h at the rotation speed of 120 plus 150rpm to prepare the fungus seed liquid;
(2) The preparation of the fungus bacterial liquid comprises the steps of adding the fungus seed liquid into a PDA liquid culture medium according to the volume ratio of 0.3-0.8%, maintaining the temperature at 25 ℃, and culturing for 2-5 days under the culture condition of the rotation speed of 120-150rpm, thus obtaining the fungus bacterial liquid.
3. The method of claim 2, wherein the bacterial species is Phanerochaete chrysosporium, Coriolus versicolor, trametes versicolor or Phlebia.
4. The method as claimed in claim 1, wherein the traditional Chinese medicine residue is one or more of radix scutellariae residue, radix isatidis residue, rhizoma acori graminei residue, radix angelicae sinensis residue and radix glycyrrhizae residue, and is mixed residue composed according to any ratio.
5. The method as claimed in claim 1, wherein the medicine residue is prepared from radix scutellariae, radix isatidis, rhizoma acori graminei, angelica sinensis and liquorice in a mass ratio of 4-6: 6-8: 6-8: 6-8: 8 to 10.
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CN107712334A (en) * | 2017-11-29 | 2018-02-23 | 衡阳香樟苑生态农业发展科技有限公司 | A kind of ox feed containing Chinese medicine dreg and preparation method thereof |
CN107751572A (en) * | 2017-12-01 | 2018-03-06 | 四川御鼎堂中药饮片有限公司 | A kind of Chinese medicine residue fermented feed for livestock and poultry and preparation method thereof |
CN107897523A (en) * | 2017-12-01 | 2018-04-13 | 四川御鼎堂中药饮片有限公司 | A kind of livestock and poultry Chinese medicine residue fermented feed and preparation method thereof |
CN108393335A (en) * | 2018-03-14 | 2018-08-14 | 北京观澜科技有限公司 | A kind of fermentation class dregs of a decoction minimizing processing method and its application |
CN108684936A (en) * | 2018-05-23 | 2018-10-23 | 宁夏绿健源生物科技有限公司 | A kind of glycyrrhiza extract fermented health-care feed and its production technology |
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