CN105842260B - A kind of discrimination method of natural taurine and synthesizing taurine - Google Patents
A kind of discrimination method of natural taurine and synthesizing taurine Download PDFInfo
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- CN105842260B CN105842260B CN201610159099.9A CN201610159099A CN105842260B CN 105842260 B CN105842260 B CN 105842260B CN 201610159099 A CN201610159099 A CN 201610159099A CN 105842260 B CN105842260 B CN 105842260B
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- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 title claims abstract description 212
- 229960003080 taurine Drugs 0.000 title claims abstract description 106
- 230000002194 synthesizing effect Effects 0.000 title claims abstract description 29
- 238000012850 discrimination method Methods 0.000 title claims abstract description 10
- 239000000523 sample Substances 0.000 claims abstract description 41
- 238000000034 method Methods 0.000 claims abstract description 26
- 238000012360 testing method Methods 0.000 claims abstract description 21
- 239000012488 sample solution Substances 0.000 claims abstract description 19
- 230000000694 effects Effects 0.000 claims abstract description 15
- 229910021642 ultra pure water Inorganic materials 0.000 claims abstract description 9
- 239000012498 ultrapure water Substances 0.000 claims abstract description 9
- 239000007788 liquid Substances 0.000 claims abstract description 7
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 claims description 9
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 claims description 9
- 238000002360 preparation method Methods 0.000 claims description 8
- 238000007689 inspection Methods 0.000 claims description 6
- 238000001514 detection method Methods 0.000 claims description 5
- CNRNYORZJGVOSY-UHFFFAOYSA-N 2,5-diphenyl-1,3-oxazole Chemical compound C=1N=C(C=2C=CC=CC=2)OC=1C1=CC=CC=C1 CNRNYORZJGVOSY-UHFFFAOYSA-N 0.000 claims description 4
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims description 4
- 239000005864 Sulphur Substances 0.000 claims description 3
- 239000002253 acid Substances 0.000 claims description 3
- 238000005259 measurement Methods 0.000 claims description 3
- -1 5- oxazolyl phenyl Chemical group 0.000 claims description 2
- 239000000243 solution Substances 0.000 description 13
- 238000003786 synthesis reaction Methods 0.000 description 7
- 241000196324 Embryophyta Species 0.000 description 6
- 241001465754 Metazoa Species 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 230000015572 biosynthetic process Effects 0.000 description 5
- 239000002994 raw material Substances 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- MIJDSYMOBYNHOT-UHFFFAOYSA-N 2-(ethylamino)ethanol Chemical compound CCNCCO MIJDSYMOBYNHOT-UHFFFAOYSA-N 0.000 description 2
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 2
- 239000003208 petroleum Substances 0.000 description 2
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- MASVCBBIUQRUKL-UHFFFAOYSA-N POPOP Chemical compound C=1N=C(C=2C=CC(=CC=2)C=2OC(=CN=2)C=2C=CC=CC=2)OC=1C1=CC=CC=C1 MASVCBBIUQRUKL-UHFFFAOYSA-N 0.000 description 1
- 206010044565 Tremor Diseases 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000004641 brain development Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003245 coal Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 230000004438 eyesight Effects 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N ferric oxide Chemical compound O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 230000004992 fission Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000000543 intermediate Substances 0.000 description 1
- 230000003907 kidney function Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000003345 natural gas Substances 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N23/00—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
The invention discloses the discrimination methods of a kind of natural taurine and synthesizing taurine.Utilize the unstable isotope of natural taurine14C activity is higher, the unstable isotope of synthesizing taurine14The very low characteristic of C activity.Taurine sample to be measured is dissolved with ultrapure water first, is then diluted to taurine solution to be measured；Again the taurine solution to be measured is uniformly mixed in equal volume with scintillation solution, is configured to testing sample solution；With Low background Liquid Scintillation Analyzer14C-channel measures the CPM value of background and testing sample solution；Result judgement is carried out according to the CPM value measured.Method of the invention is simple, accurately.
Description
Technical field
The present invention relates to the invention belongs to marine active substance detection method field more particularly to a kind of natural taurine with
The discrimination method of synthesizing taurine.
Background technique
Taurine is a kind of nonprotein amino acid of sulfur-bearing, is isolated from fel bovis earliest, in vivo with free
State exists, and is not involved in the biosynthesis of vivo protein, is a kind of conditionally essential amino acid for relying primarily on food supply.Due to
It, which has body, adjusts renal function, anti-oxidant, promotion brain development and enhancing eyesight and other effects, therefore, is widely used in
The various industries such as medicine, functional food, feed.
Currently, the production method of taurine mainly has chemical synthesis and natural extraction method.Chemical synthesis is with toxic
Ethylene oxide or 2- ethylaminoethanol be raw material, through chemical synthesis be made；Natural extraction method is from the organisms such as animals and plants
The taurine naturally extracted.It is almost harmless to human health since natural taurine derives from natural biology body, and synthesizes
Taurine due to its raw material and chemical intermediates potential hazard human health, the natural taurine market price be higher by synthesis ox
About 100 times of sulfonic acid, some illegal retailers pretend to be natural taurine with synthesizing taurine, reap staggering profits, at present in technology there has been no
Identify the method and quality system of natural taurine and synthesizing taurine, therefore natural taurine market is by severe jamming and not
Fair competition.
Summary of the invention
The purpose of the present invention is to provide a kind of simple, effective, dosage is few identification natural taurines and synthesizing taurine
Method.
To achieve the above object, the present invention provides the discrimination method of a kind of natural taurine and synthesizing taurine, feature
It is, utilizes the unstable isotope of natural taurine14C activity is high, the unstable isotope of synthesizing taurine14C activity is low,
Method to measure the CPM value of taurine sample to be measured to be determined.
Further, step are as follows:
The preparation of taurine solution to be measured: taurine sample to be measured is dissolved with ultrapure water, is then diluted to ox sulphur to be measured
Acid solution；
The preparation of testing sample solution: the taurine solution to be measured is uniformly mixed in equal volume with scintillation solution, is configured to
Testing sample solution；
The detection of CPM value: with Low background Liquid Scintillation Analyzer14The background and sample to be tested of C-channel measurement ultrapure water
The CPM value of solution；
As a result judge: result judgement is carried out according to the CPM value measured.
Further, the taurine sample is the taurine containing 98.5-101.5 dry weight %, the method for inspection used
For the content of taurine measuring method in GB14759-2010.Taurine purity is not high in taurine product of the present invention, just
Probably due to the influence of impurity, and make synthesizing taurine sample also and have and is higher14C activity.And synthesizing taurine is national on the market
Standard defines commercially available content of taurine between 98.5-101.5.Therefore the taurine sample of the present invention be containing
98.5-101.5 the taurine of dry weight %.
Further, the concentration of the taurine solution to be measured is 20-50mg/mL.
Further, the preparation method of the scintillation solution is to weigh 2,5- diphenyloxazole 4g and bis- [2- (5- oxazolyl phenyls
Base)] benzene 0.1g, is dissolved, and be settled to 1000mL with toluene.
Further, the result judgement are as follows:
When the CPM value of testing sample solution is below or near to the CPM value equal to background, institute's sample is synthesis ox sulphur
Acid；When the CPM value of testing sample solution is higher than the CPM value of background, institute's sample is natural taurine.
Unstable isotope14C is that the carbon fission in cosmic-ray bombardment earth atmosphere generates,14C and oxygen in air
After son reaction, with14CO2Form exist, should14The service life of C is 5730.14CO2With it is common12CO2It is absorbed and used by plants together
The carbohydrate such as glucose, fructose are converted into, animal, microorganism absorb the carbohydrate of plant origin as carbon source, structure
At the carbon skeleton of animal, microorganism and its metabolite, therefore in plant, animal, microorganism and its metabolite14C
Activity is higher；And fossil substance (coal, petroleum, natural gas) and its derivative (such as chemosynthesis product) are before 1 years
Animals and plants,14C activity very little, it might even be possible to ignore.Natural taurine is naturally extracted from the organisms such as animals and plants
, so14C activity is higher；Ethylene oxide or 2- ethylaminoethanol are the raw materials for producing synthesizing taurine, and both raw materials are all
It is prepared based on the fossils substance such as petroleum through complicated chemical means, so synthesizing taurine14C activity very little.
The present invention utilizes the " unstable isotope of natural taurine14C activity is higher；The unstable isotope of synthesizing taurine14C activity
It is very low " this characteristic, the invention discloses effective discrimination methods of a kind of natural taurine and synthesizing taurine.Taurine simultaneously
It is a kind of special amino acid, is single substance, chemical structure is simple, so the present invention uses the Scintillation Analyzer of Low background
Detect natural taurine and synthesizing taurine14The active nuance of C, thus show that sample taurine is natural, and also
It is synthesis.Method of the invention promotes marine economy development to have great for protecting and supervising natural taurine market
Practical significance.
The present invention is also protected based on " the unstable isotope of natural taurine14C activity is higher；The shakiness of synthesizing taurine
Determine isotope14C activity is very low " other derivative discrimination methods of this characteristic.
The CPM value refers to the umber of pulse that instrument records per minute, it is a measured value.
Advantages of the present invention: the method for the present invention is reliable and stable, and amount of samples is few, can effectively identify natural taurine and synthesis
Taurine is of great significance for protecting and supervising natural taurine market.
2 embodiments of the invention are demonstrated to be identified with method of the invention, as a result consistent with actual conditions.
Specific embodiment
The embodiment of the present invention is described below in detail, the examples of the embodiments are intended to be used to explain the present invention, and cannot
It is interpreted as limitation of the present invention.In the examples where no specific technique or condition is specified, described according to the literature in the art
Technology or conditions or carried out according to product description.Reagents or instruments used without specified manufacturer is that can lead to
Cross the conventional products of commercially available acquisition.
Scintillation solution: 2,5- diphenyloxazole (PPO flashes pure) 4g and bis- [2- (5- oxazolyl phenyl base)] benzene are weighed
(POPOP flashes pure) 0.1g, is dissolved, and be settled to 1000mL with toluene.
Embodiment 1: the identification of natural taurine and synthesizing taurine
(1) sample to be tested
Qianjiang City Yongan pharmaceutcal corporation, Ltd production of sample 1. commercially contains 98.5-101.5 dry basis
The taurine of % is measured, the content of taurine method of inspection is using the content of taurine measuring method in GB 14759-2010；
For sample 2. according to patent of invention ZL201310283654.5 method, that extracts from abalone internal organ contains 98.5-
The taurine of 101.5 dry weight %, the content of taurine method of inspection is using the content of taurine measurement in GB 14759-2010
Method.
(2) preparation of sample: it is the molten of 50mg/mL that concentration is made 1. 2. sample is dissolved with ultrapure water respectively with sample after
Liquid；
(3) detection of sample:
A, taurine solution is uniformly mixed in equal volume with scintillation solution, is configured to testing sample solution；
B, with Low background Liquid Scintillation Analyzer14C-channel measures the CPM of background (ultrapure water) and testing sample solution
Value；
(4) result judges.CPM value with the background of ultrapure aquametry is 1.9, and the CPM value of sample to be tested 1. is 1.8；To
The CPM value of sample 2. is 2.7.According to judgment principle: when the CPM value of testing sample solution is below or near to equal to background
When CPM value, institute's sample is synthesizing taurine；When the CPM value of testing sample solution is higher than the CPM value of background, institute's sample
For natural taurine.So 1. sample to be tested is synthesizing taurine, 2. sample to be tested is natural taurine.
Testing result is consistent with actual conditions.Illustrate that method testing result of the invention is accurate.
Embodiment 2: the identification of natural taurine and synthesizing taurine
(1) sample to be tested
Jiangsu Yuanyang Pharmaceutical Co., Ltd.'s production of sample 1. commercially contains 98.5-101.5 butt
The taurine of weight %, the content of taurine method of inspection is using the content of taurine measuring method in GB 14759-2010；
For sample 2. according to patent of invention ZL201310283931.2 method, that extracts from mottle clam contains 98.5-101.5
The taurine of dry weight %, the content of taurine method of inspection is using the content of taurine measuring method in GB 14759-2010.
(2) preparation of sample: it is 20mg/mL that concentration, which is made, in dilution 1. 2. sample is dissolved with ultrapure water respectively with sample after
Solution；
(3) detection of sample:
A, taurine solution is uniformly mixed in equal volume with scintillation solution, is configured to testing sample solution；
B, with Low background Liquid Scintillation Analyzer14C-channel measures the CPM of background (ultrapure water) and testing sample solution
Value；
(4) result judges.CPM value with the background of ultrapure aquametry is 1.9, and the CPM value of sample to be tested 1. is 1.9；To
The CPM value of sample 2. is 2.5.According to judgment principle: when the CPM value of testing sample solution is below or near to equal to background
When CPM value, institute's sample is synthesizing taurine；When the CPM value of testing sample solution is higher than the CPM value of background, institute's sample
For natural taurine.So 1. sample to be tested is synthesizing taurine, 2. sample to be tested is natural taurine.
Testing result is consistent with actual conditions.Illustrate that method testing result of the invention is accurate.
Although the embodiments of the present invention has been shown and described above, it is to be understood that above-described embodiment is example
Property, it is not considered as limiting the invention, those skilled in the art are not departing from the principle of the present invention and objective
In the case where can make changes, modifications, alterations, and variations to the above described embodiments within the scope of the invention.
Claims (3)
1. the discrimination method of a kind of natural taurine and synthesizing taurine, which is characterized in that step are as follows:
The preparation of taurine solution to be measured: taurine sample to be measured is dissolved with ultrapure water, it is molten to be then diluted to taurine to be measured
Liquid；The taurine sample is the taurine containing 98.5-101.5 dry weight %, and the method for inspection used is GB 14759-
Content of taurine measuring method in 2010
The preparation of testing sample solution: the taurine solution to be measured is uniformly mixed in equal volume with scintillation solution, is configured to be measured
Sample solution；
The detection of CPM value: with Low background Liquid Scintillation Analyzer14The background and testing sample solution of C-channel measurement ultrapure water
CPM value；
As a result judge: utilizing the unstable isotope of natural taurine14C activity is high, the unstable isotope of synthesizing taurine14C
Activity is low, thus the method for measuring the CPM value of taurine sample to be measured to be determined；Result is carried out according to the CPM value measured
Judgement: when the CPM value of testing sample solution is less than or equal to the CPM value of background, institute's sample is synthesizing taurine；When to be measured
When the CPM value of sample solution is higher than the CPM value of background, institute's sample is natural taurine.
2. the discrimination method of natural taurine described in claim 1 and synthesizing taurine, which is characterized in that the ox sulphur to be measured
The concentration of acid solution is 20-50mg/mL.
3. the discrimination method of natural taurine described in claim 1 and synthesizing taurine, which is characterized in that the scintillation solution
Preparation method is to weigh 2,5- diphenyloxazole 4g and bis- [2- (5- oxazolyl phenyl base)] benzene 0.1g, is dissolved with toluene, and constant volume
To 1000mL.
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| CN112578027A (en) * | 2020-11-25 | 2021-03-30 | 河南染匠科技有限公司 | Method for identifying natural dye and synthetic dye and application thereof |
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| CN103342668A (en) * | 2013-07-08 | 2013-10-09 | 集美大学 | Simple method for extracting natural taurine from abalone viscera |
| CN103342669A (en) * | 2013-07-08 | 2013-10-09 | 集美大学 | Simple method for extracting natural taurine |
| CN104725284A (en) * | 2015-03-06 | 2015-06-24 | 集美大学 | Novel preparation method for natural taurine |
| CN105272887A (en) * | 2015-10-20 | 2016-01-27 | 集美大学 | Method for extracting taurine and polysaccharides from abalone's viscera simultaneously |
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| WO2011098979A3 (en) * | 2010-02-12 | 2011-10-20 | Commissariat A L'energie Atomique | Radioresistant alga of the coccomyxa genus |
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| CN103342668A (en) * | 2013-07-08 | 2013-10-09 | 集美大学 | Simple method for extracting natural taurine from abalone viscera |
| CN103342669A (en) * | 2013-07-08 | 2013-10-09 | 集美大学 | Simple method for extracting natural taurine |
| CN104725284A (en) * | 2015-03-06 | 2015-06-24 | 集美大学 | Novel preparation method for natural taurine |
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