CN105838496A - Method for preparing edible oil with comprehensive nutrition by using oil cake obtained after oil expression - Google Patents
Method for preparing edible oil with comprehensive nutrition by using oil cake obtained after oil expression Download PDFInfo
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- CN105838496A CN105838496A CN201610225667.0A CN201610225667A CN105838496A CN 105838496 A CN105838496 A CN 105838496A CN 201610225667 A CN201610225667 A CN 201610225667A CN 105838496 A CN105838496 A CN 105838496A
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/04—Pretreatment of vegetable raw material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
- A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
- A23D9/02—Other edible oils or fats, e.g. shortenings, cooking oils characterised by the production or working-up
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
- C11B1/108—Production of fats or fatty oils from raw materials by extracting after-treatment, e.g. of miscellae
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Fats And Perfumes (AREA)
- Edible Oils And Fats (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention provides a method for preparing edible oil with comprehensive nutrition by using oil cake obtained after oil expression. The method comprises crushing the oil cake obtained after oil expression, adding electrolysed water to perform supersonic extraction, performing organic solvent extraction, taking an organic layer to heat the layer, centrifuging the mixture at low temperature, collecting the interface layer clear liquid, evaporating the organic solvent, and drying the obtained material to obtain the edible oil, wherein the oil is rich in functional components such as linoleic acid, linolenic acid, EPA, DHA, and nervonic acid. The contents of the linoleic acid and linolenic acid in the grease extracted from the part of the oil cake can reach tens times of the corresponding oil types of the market oil, and the edible oil is rich in EPA, DHA, and nervonic acid which are absent of the oil type of the market oil. The method can improve the economic benefit of the enterprise greatly, improve the health level of our people, improve the utilization rate of the bulk oil material, and relatively increase the grease output of our country, so that the grease import is reduced, and grease supplying level and quality of our country are guaranteed from our grease strategy. The method has great practical significance and profound strategic significance.
Description
Technical field
The present invention relates to food engineering field, specifically, relate to one and utilize bulk edible oil
The method that after squeezing, natural full nutrient edible oil prepared by discarded oil cake.
Background technology
Edible oil is one of indispensable food of our daily life, relates to huge numbers of families, its
Quality and quality are related to the health of each eater；Grease or national strategy maintenance variety,
The a lot of large oil plant of China all relies on external import at present, and such as external imported soybean is because of cost
Cheap, in China's soya-bean oil processing and production, occupy critical positions.At present, bulk edible oil
Preparation is many is all that the seed of oil crops is through the acquisition of conventional press method.
Traditional oil expression mode mainly has hot moulding method and cold-press, hot moulding method grease obtained smell virtue
Perfume (or spice), yield is high, easily preserves, although but owing to hot moulding method cost is relatively low, productivity is higher, but
Its temperature is higher, and in edible oil, a lot of effective efficiency compositions are the most destroyed, or cannot be introduced into pressure
After squeezing in oil phase.It is strong that cold-press shortcoming is that smell is not so good as hot moulding method, but color and luster is preferable, additionally
Although cold-press oil yield only has the half of hot moulding method, the requirement to equipment is the highest, along with battalion
Supporting the progress of health science, relatively costly cold-press is increasingly subject to welcome, and its range of application obtains
Arrive expansion.Although it addition, modern extraction process develops again outside hot moulding method and cold-press
Lixiviation process, i.e. enters by adding chemical substance (typically organic solvent) in oil plant raw material
Row extracts, but owing to the method is higher to organic solvent and technological requirement, it is easy to cause poisonous
Harmful substance remains in finished product grease, causes damage consumer health.
Cold-press is related, and invents before more than 700 years, and the method has prominent advantage,
Belong to physical method, it is not necessary to worry chemical residual, simple to operate, therefore, use till today always.
But, along with the development of medical health care science and technology, life and the raising of the general level of the health, nutrition
The number of teacher increases year by year, and modern increasingly payes attention to the pass of food nutrition and the health taken in
System, it is generally recognized that the oil phase nutritional labeling that cold-press is obtained retains more complete, is worth higher,
Thus cold-press is currently increasingly subject to grease production processing producer and the welcome of consumers in general.
Under this overall background, inventor in a large amount of actual productions and scientific research, surprisingly it has been found that,
For a long time, tradition is considered the aliphatic acid composition of the most complete pressed oil of nutritional labeling reservation
And imperfect, in its aliphatic acid composition and corresponding oil cake, the aliphatic acid composition of oil has heaven and earth
Other, and modern science thinks that the aliphatic acid to human body with important physiological function is present in the greatest extent
In oil cake, in other words, although cold-press can relatively efficiently keep nutritional labeling, but actual
Upper major part nutritional labeling is also not remain in oil phase namely the obtained oil that cold-press is obtained
In fat, and being kept in the oil cake discarded subsequently, this causes selecting cold-press to deviate from the beginning of it
Inner feelings.
Inventor finds, especially for soya-bean oil, peanut oil, rapeseed oil, Semen Benincasae oil, corn
For oil, sesame oil, linseed oil and shinyleaf yellowhorn oil etc., use the grease that obtained of cold-press and
The grease composition retained in corresponding oil cake is compared, and aliphatic acid composition exists the biggest difference, especially
It is, a lot of essential fatty acids, so the aliphatic acid that human body is had critical function of discovered in recent years,
It is distributed in oil cake in a large number, and content is little in oil and fat product.
The health of human body can be played regulation effect by functional components contained in grease.Its
Include the necessary aliphatic acid of human body, as linoleic acid, leukotrienes, arachidonic acid, DHA,
EPA etc..Human body can not synthesize linoleic acid plus linolenic acid, it is necessary to supplements from meals, and people
Body may utilize the much indispensable aliphatic acid of human body of linoleic acid plus linolenic acid synthesis.EPA has
Reducing blood lipid, platelet aggregation-against, delay thrombotic effect.DHA have regulation blood fat,
Immunological regulation, safeguard that retina improves eyesight, mends the effect such as brain brain tonic.Due to traditional squeezing
A lot of functional components of grease are remained in cake by method, can not get for a long time eating from edible oil
Oil natural full nutrient.Therefore, the task of top priority needs to be used for adjusting back edible oil by functional fatty acid,
Improve edible oil trophic structure, improve the trophic level of edible oil.These functional components also may be used
For fields such as health medicine, cosmetics, surface-active raw materials, can not only be for improving oil cake
A new breakthrough mouth is found in comprehensive utilization, and can also open up for improving the economic worth of oil cake
Article one, new way.Show according to State Statistics Bureau, national oil crops such as peanut, vegetable seed in 2013
Isoquant just reaches 35,170,000 tons, will produce 40%～50% left after the oil expression of these oil crops
Right cake, this stock number is the hugest.These cakes are used for producing various bean product except soybean cake
Outward, other majority is used for making low side food and feeding, although achieve certain effect, but
The most scattered, many large oil cakes are all underutilized.Existing exploitation
The enterprise of oil cake is substantially to produce single product, is formed without comprehensive development and utilization whole
The process route of body planning and series of products, research and development field also lacks comprehensive development and utilization
Supporting technology, various reasons causes the comprehensive development and utilization of cake still in the primary stage, does not obtains
Utilize to effective.
Summary of the invention
Being found by the research of inventor, conventional press technique much will have physiological function to human body
Aliphatic acid remains in oil cake, does not simply fail to be obtained the full nutrition of natural oil by edible oil,
And make China's only oil resource significant wastage.Therefore, it is an object of the invention to provide one
Plant the discarded oil cake obtained from conventional press method, especially cold-press, the most large oil cake
(gained oil cake after i.e. extracting oil from soybean, peanut, sunflower, vegetable seed, sesame, corn etc.)
Middle extraction contains the method for the grease of functional fatty acid.
In order to realize the object of the invention, the invention provides gained oil cake system after a kind of utilization oil expression
The method of the standby edible oil comprising functional fatty acid composition, after described oil expression, gained oil cake is preferred
From large oil cake, the method comprises the following steps:
(1) oil cake after being extracted oil by cold-press adds the electrolysis ultrasonic extraction of water after pulverizing；
(2) organic solvent extraction, stirring, heating；
(3) low-temperature centrifugation, collects, extracts organic layer clear liquid；
(4) evaporation of organic solvent, gained material is dried, and obtains oil and fat product.
Wherein, step (1) presses solid-liquid ratio addition pH value 2-12 of 1:8～15 in oil cake powder
Electrolysis water, carry out power 50W-700W, the ultrasound assisted extraction of 10-40min.
Preferably, step (1) presses solid-liquid ratio addition pH value 2-12 of 1:10 in oil cake powder
Electrolysis water.
Obtained by oil and fat product in be difficult to obtain containing cold-press linoleic acid, leukotrienes,
EPA, DHA, and/or nervonic acid.
The preparation method being electrolysed water described in step (1) is: occurred by acidic oxidized electric potential water
Device cleans up, and two parts of precise 2.5g sodium chloride, is respectively put into alkalescence water pot and acidity
In water pot, and being separately added into the distilled water of 1L, stirring makes sodium chloride fully dissolve；
Above-mentioned solution is poured into respectively the left groove of generator and right groove, plugs in, set and run
Time is 15min, and the sound that ticks collects electrolyzed alkaline water and pH≤2 of pH >=12 automatically after crossing
Acidic electrolytic water；
Draw above-mentioned electrolysis water, carry out acid-base neutralization, make respectively pH be 2,4,7,10,
The electrolysis water of 12.
Wherein, the organic solvent used in step (2) selected from 60 DEG C～90 DEG C boiling ranges petroleum ether,
N-hexane, or the petroleum ether of 30 DEG C～60 DEG C boiling ranges.
Wherein, in step (2), the volume mass ratio of organic solvent and oil cake powder is 10～25:1.
Preferably, in step (2) volume mass of organic solvent and oil cake powder than for 20:1.
Further, the method extracting organic layer heating in step (2) is organic by extract
Layer after magnetic agitation 20～30min, heats 40～60min at 40～50 DEG C.
Preferably, the method extracting organic layer heating in step (2) is the organic layer that will extract
At 50 DEG C after magnetic agitation 25min, heat 50min.
In the inventive method, in step (3), centrifugal rotational speed is 5500～6500rpm, the time
15～20min.
Preferably, in step (3), centrifugal rotational speed is 6000rpm, time 20min.
In the inventive method, using Rotary Evaporators to be evaporated in step (4), temperature is steamed in rotation
Degree is 55～65 DEG C.
Preferably, using Rotary Evaporators to be evaporated in step (4), it is 60 that temperature is steamed in rotation
℃。
Wherein, step (4) is dried after petroleum ether in Rotary Evaporators, gained material in
90～105 DEG C are dried to constant weight.
Preferably, after step (4) is dried petroleum ether in Rotary Evaporators, gained material
It is dried to constant weight in 105 DEG C.
Further, the inventive method also includes step (5), is by step (4) gained oil
Fat prod and the grease obtained mixing of cold-press, thus obtain the edible oil of comprehensive nutrition.
It is highly preferred that the inventive method also includes step (6): will be from variety classes oil plant
The edible oil through said method gained comprehensive nutrition prepare together, obtain allocate edible oil.
Preferably, the method for the present invention, can carry out according to following program: gained after extracting oil
Oil cake is dried, pulverizes, obtains oil cake powder；The material of 1:10 is pressed in oil cake powder
Liquor ratio adds the electrolysis water of pH value 2-12, carries out power 50W-700W, 10-40min's
Ultrasound assisted extraction；Utilize 60 DEG C～90 DEG C boiling ranges petroleum ether extraction [extractant (v):
Oil cake (m)=20:1], extract and complete take organic layer magnetic agitation 25min at 50 DEG C
After, 50 DEG C of heating 50min, carry out low-temperature centrifugation (6000rpm, 20min), collect middle
Layer clear liquid, 60 DEG C of rotary evaporations are dried petroleum ether, gained material be dried (105 DEG C) to constant weight,
GC-MS analyze draw grease at least contain one functional fatty acid composition, as linoleic acid,
Leukotrienes, EPA, DHA, nervonic acid.
The technique using the present invention to provide can more sufficiently extract the function of residual in conventional method
Property Fatty acid component, inventor pass through great many of experiments, find the present invention extract from discarded oil cake
Edible oil compare the grease directly extracted from same raw material by conventional press method for, effectively
Composition functional components kind more horn of plenty in other words, and content is higher.As peanut cake oil compares phase
(Erucic acid, also known as 22 for answered peanut pressed oil many two kinds of fatty acid compositions, i.e. erucic acid
-13-olefin(e) acid；22 carbon-13-olefin(e) acids) and nervonic acid (cis-15-Tetracosenoic acid,
cis-15-Teracosenoic acid；(15Z)-tetracos-15-enoic acid, also known as cis-15-20
Four carbon enoic acids；Selacholeic acid), content is respectively 7.23%, 0.56%, arachidic acid (Eicosanoic
Acid, Icosanoic Acid, also known as positive 20 acid, arachic acid, positive structure twenty acid), arachidonic
Acid (Arachidonic acid, also known as arachidonic acid, all-cis formula-5,8,11,14-bis-ten carbon four
Olefin(e) acid, AA or ARA) content is the 44.36 of former pressed oil, 5.14 times respectively；Maize germ
Stearic acid, oleic acid and arachidic acid content in cake oil is the 1.60 of former pressed oil respectively, 1.25,
1.26 again；Sunflower cake oil extracted gaidic acid (0.07%) that the former pressed oil of sunflower do not has,
Tricosenoic acid (0.23%), nervonic acid (0.10%), DHA (0.48%)；Soybean
Cake is extracted 14 kinds of Fatty acid components, pressed oil more former than soybean is many 2 kinds of compositions, i.e. mustard
Acid (4.70%) and nervonic acid (0.51%), stearic acid in cake oil, oleic acid, arachidic acid,
The acid of peanut monoenoic acid, eicosapentaenoic acid, leukotrienes, tetracosa carbon, Heptadecanoic acide, monkshood
Resin acid, arachidonic acid content be the 1.18 of former pressed oil aliphatic acid respectively, 1.20,1.11,
2.45,2.67,2.81,4.42,10.5,10.88,42.0 times, equilibrium is compared in nutrition；Vegetable seed
Having measured the eicosadienoic acid not having in former pressed oil and tetracosa carbon acid in cake oil, content is respectively
1.58% and 0.29%.
It addition, the present invention is relatively found by grease ratio of components between different oil kinds, people are at daily drink
Food is difficult to obtain the nutrition of equilibrium from same oil, if being eaten for a long time same oil, easily
Cause malnutrition, it is thus achieved that new science data support, therefore in diet at ordinary times it should be noted that
The collocation of multiple eating oil, or edible blend oil.Such as they linoleic acid contents are by the most at least
Successively: sunflower cake oil (61.56%), maize germ cake oil (48.02%), sesame-send cake oil
(42.66%), shiny-leaved yellowhorn cake oil (41.84%), soybean cake oil (32.57%), peanut
Cake oil (30.45%), rape cake oil (23.35%), oil cake of flax seed oil (19.61%), can
Seeing, between material to be tested, linoleic acid content is widely different, and the linoleic acid content of sunflower cake oil is flax
3.14 times of cake oil；Linolenic acid content is followed successively by from low to high: sunflower cake oil (0.21%),
Rape cake oil (13.22%), oil cake of flax seed oil (43.67%), other does not detects；EPA
Content is followed successively by from high to low: peanut cake oil (1.78%), soybean cake oil (1.20%), literary composition
Hat fruitcake oil (0.51%), rape cake oil (0.41%), sesame-send cake oil (0.13%), certain herbaceous plants with big flowers
Flower cake oil (0.06%), in peanut cake oil, the content of EPA is 29.67 times of sunflower cake oil,
It follows that EPA content difference is the biggest between different cakes；Material to be tested only has corn-dodger
Oil and sunflower cake oil have detected DHA, but content is less.The present invention can also be deep sea fish oil
Rich in the nutrition of this high value of EPA and DHA extract from oil plant oil cake, this
Many-side is respectively provided with important meaning.Therefore, the present invention makes edible oil trophic structure obtain greatly
Improvement, for improve our people's general level of the health be significant.The large oil of Ye Shi China
The comprehensive utilization of cake, the economic worth of raising oil cake open up a new way.
Additionally, in addition to effect mentioned above, through lot of experiments, inventor finds this
Bright also have the advantage that
After what the present invention provided utilize oil expression, gained oil cake is prepared the edible oil of comprehensive nutrition and (is contained
Functional fatty acid) method, raw material sources are extensive, and method of operating is simple and easy, to environment
Close friend, can more sufficiently extract the functional fatty acid composition that conventional press method remains in cake,
Wherein, the linoleic acid content in soybean cake oil, maize germ cake oil, sesame-send cake oil is phase respectively
Answer 66.47 times of commercially available oil, 57.86 times, 51.40 times, the linoleic acid in other four kinds of cake oil
Content is 1.11～1.71 times of corresponding commercially available oil；Asia in sunflower cake oil and rape cake oil
Fiber crops acid is 21 times and 5.58 times of corresponding commercially available oil respectively；Peanut cake, soybean cake, rape cake,
All proposing the EPA not having in corresponding commercially available oil in sesame-send cake, sunflower cake, its content exists
Between 0.06%～1.78%；It addition, also propose DHA from corn-dodger and sunflower cake,
Its content is respectively 0.05%, 0.48%, but, the commercially available corn oil that same company produces
Do not contain with sunflower oil, also have and propose what corresponding commercially available oil did not had from peanut cake and soybean oil
Nervonic acid, content is respectively 0.56%, 0.51%.
Therefore, it is found that the present invention is worth the side being not only to extract from these are tested
Method itself, and be to find that the mankind do not obtain natural full battalion from bulk edible oil for these years
Support！
The present invention, compared with traditional chemical reagent, uses electrolysis water pretreatment can reach same
The extraction conditions of pH value, meanwhile, utilize ultrasonic assistant to extract, it is possible to make cavitation
Strengthen, be sufficiently destroyed cell membrane, the dissolution of the most more greases, excellent by both of the method
Gesture combines, and is combined optimization in view of both reciprocation, use electrolysis water and
Ultrasonic assistant extracts the grease of large oil cake, while improving recovery rate, it is to avoid uses and passes
The pollution that the chemical reagent of system brings；The waste liquid got rid of in process of production to environment without any dirt
Dye, noresidue；Produce conveniently, with low cost.
Organic solvent extractionprocess is the principle of application liquid-solid extraction, selects certain can dissolve grease
Organic solvent, through contact to oil plant, make the one side that in oil plant, grease is extracted out
Method.The present inventor is by tens of kinds of organic solvents of contrast, by great many of experiments, screening
Go out above organic solvent, and found that the petroleum ether using 60 DEG C～90 DEG C boiling ranges extracts grease,
Active ingredient in grease can be made not to be destroyed, and simple to operate, oil yield is compared to other
Solvent (n-hexane, the petroleum ether of 30 DEG C～60 DEG C boiling ranges) is higher, and boiling point is low to be easily recycled instead
Multiple utilization, not residual.
To sum up, finding according to the test of inventor, a kind of oil plant is through traditional handicraft cold press
Method is prepared grease and is not obtained whole nutrition of lipids, and still contains substantial amounts of in its oil cake
The grease of the high nutritive value that carbochain is longer, could obtain natural by the former and the mixing of the latter's grease
Full nutrient edible oil；On this basis, further can be with the full nutrient edible oil of such a with another
The full nutrient edible oil in one or more different oil plant sources is configured to edible health-care oil, thus can
Obtain the complementation of grease fatty acid nutritive contained by different oil plant.
The experiment of the present invention relate to large oil crops of more than 8 kinds, cover all of greatly
Ancestor oil crops, practicality is extremely strong, and meaning is the hugest, and the warp of enterprise is not only greatly improved
Ji benefit, and for improving our people's general level of the health, improving the utilization rate of large oil plant,
Relatively increase China's grease yield, thus reduce grease import, ensure from China's grease strategy
Domestic grease level of supply and quality, be of great immediate significance and far-reaching strategic importance.
Detailed description of the invention
Following example are used for illustrating the present invention, but are not limited to the scope of the present invention.If not
Specialize, the routine that technological means used in embodiment is well known to those skilled in the art
Means, the raw materials used commercial goods that is, the content ratio gone out given in embodiment is as without especially
Mark, then be weight ratio.
The preparation method being electrolysed water in following example is: by clear for acidic oxidized electric potential water generator
Wash clean, and two parts of precise 2.5g sodium chloride, be respectively put into alkalescence water pot and sour water tank
In, and it being separately added into the distilled water of 1L, stirring makes sodium chloride fully dissolve.Above-mentioned solution is divided
Do not pour the left groove of generator and right groove into, plug in, set the operation time as 15min, tick
The sound collects electrolyzed alkaline water (pH >=12) and acidic electrolytic water (pH≤2) automatically after crossing.
Above-mentioned electrolysis water will be drawn, carry out acid-base neutralization, make respectively pH be 2,4,7,10,
The electrolysis water of 12.
Embodiment 1 is extracted containing functional fatty acid from large oil cake (maize germ cake)
The method of grease
The electrolysis water of pH 10 is joined in the powder of maize germ oil cake with solid-liquid ratio 1:10,
Carry out power 150W, the ultrasound assisted extraction of 30min.Oil by 60 DEG C～90 DEG C boiling ranges
Ether extraction (extractant (v): oil cake (m)=20:1), takes organic layer, at 50 DEG C
After lower magnetic agitation 25min, 50 DEG C heating 50min, carry out low-temperature centrifugation (6000rpm,
20min), collecting supernatant, 60 DEG C of rotary evaporations are dried petroleum ether, and gained material is dried (105
DEG C) to constant weight.Oil extracting rate is 7.48%.Maize germ cake oil is carried out GC-MS analysis, look
Spectrum post: CNW CD-ACIDWAX capillary column (30m × 250 μm, 25 μm)；Intensification journey
Sequence: initial temperature 150 DEG C, with persistently overheating to 190 DEG C of 8 DEG C/min, keeps 3min, then
It is warming up to 240 DEG C with 10 DEG C/min, keeps 13min, run 25min altogether；Hydrogen flowing quantity:
40.0mL/min, air mass flow: 450.0mL/min；Sample size 1.00 μ L；Split ratio: 30:1；
Injector temperature: 280 DEG C；Detector: fid detector, detector temperature: 300 DEG C.Mirror
Making containing linoleic acid and DHA, both content are respectively 48.02% and 0.05%.
Embodiment 2 extracts the oil containing functional fatty acid from large oil cake (sunflower oil cake)
The method of fat
The electrolysis water of pH 10 is joined with solid-liquid ratio 1:10 in the powder of sunflower oil cake, carry out
The ultrasound assisted extraction of power 150W, 30min.Petroleum ether extraction by 60 DEG C～90 DEG C boiling ranges
[extractant (v): oil cake (m)=20:1], takes organic layer magnetic force at 50 DEG C and stirs
After mixing 25min, 50 DEG C of heating 50min, carry out low-temperature centrifugation (6000rpm, 20min), receive
Collection supernatant, 60 DEG C of rotary evaporations are dried petroleum ether, and gained material is dried (105 DEG C) to constant weight.Carry
Oil rate is 3.25%.Sunflower cake oil is carried out GC-MS analysis (the same embodiment of testing conditions parameter
1), identify containing linoleic acid, leukotrienes, EPA, DHA, content is respectively 61.56%,
0.21%, 0.06%, 0.48%.
Embodiment 3 extracts the oil containing functional fatty acid from large oil cake (soybean oil cake)
The method of fat
The electrolysis water of pH 10 is joined with solid-liquid ratio 1:10 in the powder of soybean oil cake, carry out
The ultrasound assisted extraction of power 150W, 30min.Petroleum ether extraction by 60 DEG C～90 DEG C boiling ranges
[extractant (v): oil cake (m)=20:1], takes organic layer magnetic force at 50 DEG C and stirs
After mixing 25min, 50 DEG C of heating 50min, carry out low-temperature centrifugation (6000rpm, 20min), receive
Collection supernatant, 60 DEG C of rotary evaporations are dried petroleum ether, and gained material is dried (105 DEG C) to constant weight.Carry
Oil rate is 3.23%.Soybean cake oil is carried out GC-MS analysis (the same embodiment of testing conditions parameter
1), identify containing linoleic acid, leukotrienes, EPA, nervonic acid；Content is respectively 32.57%,
2.48%, 1.20%, 0.51%.
Embodiment 4 extracts the oil containing functional fatty acid from large oil cake (rap oil seedcake)
The method of fat
The electrolysis water of pH 10 is joined with solid-liquid ratio 1:10 in the powder of rap oil seedcake, carry out
The ultrasound assisted extraction of power 150W, 30min.Petroleum ether extraction by 60 DEG C～90 DEG C boiling ranges
[extractant (v): oil cake (m)=20:1], takes organic layer magnetic force at 50 DEG C and stirs
After mixing 25min, 50 DEG C of heating 50min, carry out low-temperature centrifugation (6000rpm, 20min), receive
Collection supernatant, 60 DEG C of rotary evaporations are dried petroleum ether, and gained material is dried (105 DEG C) to constant weight.Carry
Oil rate is 7.15%.Rape cake oil is carried out GC-MS analysis (the same embodiment of testing conditions parameter
1), identify containing linoleic acid, leukotrienes, EPA, nervonic acid；Content is respectively 23.35%,
13.22%, 0.41%, 1.51%.
Embodiment 5 extracts the grease containing functional fatty acid from large oil cake (sesame oil cake)
Method
The electrolysis water of pH 10 is joined with solid-liquid ratio 1:10 in the powder of sesame oil cake, carry out
The ultrasound assisted extraction of power 150W, 45min.Petroleum ether extraction by 60 DEG C～90 DEG C boiling ranges
[extractant (v): oil cake (m)=20:1], takes organic layer magnetic force at 50 DEG C and stirs
After mixing 25min, 50 DEG C of heating 50min, carry out low-temperature centrifugation (6000rpm, 20min), receive
Collection supernatant, 60 DEG C of rotary evaporations are dried petroleum ether, and gained material is dried (105 DEG C) to constant weight.Carry
Oil rate is 13.44%.Sesame-send cake oil carries out GC-MS analysis, and (testing conditions parameter is with implementing
Example 1), identify containing linoleic acid, EPA；Content is respectively 41.85%, 0.13%.
Embodiment 6 extracts the oil containing functional fatty acid from large oil cake (flax oil cake)
The method of fat
1) the electrolysis water of pH 13 is joined with solid-liquid ratio 1:10 in the powder of flax oil cake, enter
Row power 150W, the ultrasound assisted extraction of 30min.Extract with the petroleum ether of 60 DEG C～90 DEG C boiling ranges
Take [extractant (v): oil cake (m)=20:1], take organic layer magnetic force at 50 DEG C
After stirring 25min, 50 DEG C of heating 50min, carry out low-temperature centrifugation (6000rpm, 20min),
Collect supernatant, 60 DEG C of rotary evaporations are dried petroleum ether, gained material be dried (105 DEG C) to constant weight,
Oil extracting rate is 2.27%.Oil cake of flax seed oil carries out GC-MS analysis, and (testing conditions parameter is with implementing
Example 1), identify containing linoleic acid, leukotrienes, nervonic acid.Content is respectively 19.61%,
43.67%, 0.19%.
From the above results, the method that the present invention provides can extract in large oil cake and contain
The grease of functional fatty acid composition.
Although, the most with a general description of the specific embodiments the present invention has been made in detail
Most description, but on the basis of the present invention, it can be made some modifications or improvements, this is to this
It is apparent from for skilled person.Therefore, on the basis without departing from spirit of the present invention
Upper these modifications or improvements, belong to the scope of protection of present invention.
Claims (9)
1. the method that after utilizing oil expression, the edible oil of comprehensive nutrition prepared by gained oil cake, its
It is characterised by, comprises the following steps:
(1) oil cake after being extracted oil by cold-press adds the electrolysis ultrasonic extraction of water after pulverizing；
(2) organic solvent extraction, stirring, heating；
(3) low-temperature centrifugation, collects, extracts organic layer clear liquid；
(4) evaporation of organic solvent, gained material is dried, and obtains oil and fat product.
Method the most according to claim 1, it is characterised in that step (1) is to oil cake
The solid-liquid ratio pressing 1:8～15 in powder adds the electrolysis water of pH value 2-12, carries out power
The ultrasound assisted extraction of 50W-700W, 10-40min.
Method the most according to claim 1, it is characterised in that step (2) is organic molten
Agent is selected from the petroleum ether of 60 DEG C～90 DEG C boiling ranges, n-hexane, or the petroleum ether of 30 DEG C～60 DEG C boiling ranges.
Method the most according to claim 3, it is characterised in that organic in step (2)
Solvent is 10～25:1 with the volume mass ratio of oil cake powder.
Method the most according to claim 1, it is characterised in that the method for step (2)
Organic layer for extracting after magnetic agitation 20～30min, heats 40-60 at 40～50 DEG C
min。
Method the most according to claim 1, it is characterised in that centrifugal in step (3)
Rotating speed is 5500～6500rpm, the time 15～20min.
Method the most according to claim 1, it is characterised in that use in step (4)
Rotary Evaporators is evaporated, and it is 55～65 DEG C that temperature is steamed in rotation.
Method the most according to claim 7, it is characterised in that in rotation in step (4)
Turning after being dried petroleum ether in evaporimeter, gained material is dried to constant weight in 90～105 DEG C.
9. according to the arbitrary described method of claim 1-8, it is characterised in that grease obtained product
Mixing grease obtained with milling process, thus obtain the edible oil of comprehensive nutrition.
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| CN108531284A (en) * | 2018-03-15 | 2018-09-14 | 河南亨通粮油食品有限公司 | A kind of production method of high-quality sesame oil |
| CN109077185A (en) * | 2018-08-28 | 2018-12-25 | 湖南大三湘茶油股份有限公司 | The preparation method of natural saccharicter-penin |
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