CN1058293C - Chinese herb medicine extraction by enzymolysis - Google Patents
Chinese herb medicine extraction by enzymolysis Download PDFInfo
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- CN1058293C CN1058293C CN93120161A CN93120161A CN1058293C CN 1058293 C CN1058293 C CN 1058293C CN 93120161 A CN93120161 A CN 93120161A CN 93120161 A CN93120161 A CN 93120161A CN 1058293 C CN1058293 C CN 1058293C
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Abstract
The present invention belongs to enzyme method extraction of traditional Chinese medicine, which discloses a biologic extraction method of traditional Chinese medicine. The present invention relates to a use method for using different combinations of enzymes and using different enzymes according to the medical properties of specific traditional Chinese medicine. The present invention is characterized in that the characteristics of the high catalytic property, the strong specificity, the mild action conditions, the nontoxicity, etc. of enzymes are used for extracting the medicine effect components of traditional Chinese medicine. The present invention is widely suitable for unilateral preparations and compound preparations of traditional Chinese medicine.
Description
The present invention relates to and in the producing of Chinese medicine, use zymin, force catalystic, fermentative to extract middle pharmaceutically active ingredient, for the biological extraction of Chinese medicine preparation provides a novel process.
Chinese materia medica is an important wealth of world medicine treasure-house.Chinese medicine preparation is of long standing and well established, and soup starts from (B.C. 1766) before the Shang dynasty.The modern Chinese herbal medicine preparation has one to overlap leach extraction method comparatively complete, system, as decocting method, percolation, reflux extraction etc.The purified method comprises water extraction and alcohol precipitation method, ethanol extract from water precipitation, liquid-liquid extraction method, acid-base method, salting-out process, dialysis method, sieve method, polymeric amide absorption method, ion exchange method, crystallization process etc.Xiao Helong has invented (publication number CN1064888A. application number 92102469.Y. volume issue 8-40) edible mushrooms and herbal medicine mixed fermentation brewing process.
The objective of the invention is to: utilize characteristics such as strong, gentle action condition of biological enzyme formulation catalytic rate height, specificity and nontoxicity, a large amount of herbal medicine is carried out serial enzymes such as catalyzed degradation, synthetic, transfer and turn usefulness into, improve the extraction yield and the utilization ratio of Chinese medicine with this, promote the development of Chinese drugs agentia production technology.
The protein with katalysis that is produced by cell is called enzyme.According to the type of various enzyme institute catalyzed reaction, enzyme can be divided into six big classes, and wherein: the hydrolase major part belongs to extracellular enzyme, it is the widest to distribute in vivo, quantity is maximum, and industrial production and application are also the most extensive, as cellulase, polygalacturonase, amylase, proteolytic enzyme, nuclease etc.Because the composition of some biologically active generally is low molecular compound or can be dissociated into the ionic material at the aqueous solution in the Chinese medicine extract, so all can use lytic enzyme to general Chinese medicine.According to cellulase-→ polygalacturonase-→ amylase-→ proteolytic enzyme-→ order of nuclease, then can be with Mierocrystalline cellulose firm in structure in the root of Chinese medicine (draft, xylophyta), stem, leaf, flower, fruit, the seed, degradeds step by step such as the pectin of height fatization and pectic acid, starch, albumen, nucleic acid finally generate all kinds of low molecular compounds.Otherwise Chinese medicine has the polysaccharide, protein, tannin of therapeutic action etc., then mainly uses cellulase and polygalacturonase, and as need nucleic acid degradation product Nucleotide, nucleosides and phosphoric acid etc., then emphasis uses proteolytic enzyme and nuclease.The effect of a kind of enzyme of single use is unsatisfactory, must the logotype plurality of enzymes.As substrate, then must understand and study the structure of matter and the characteristic of this Chinese medicine with specific Chinese medicine, could select then with histaminase to its catalyzed degradation.
The present invention compares the advantage that is had with prior art and is: can use the enzyme of various combination to carry out catalyzed degradation according to the characteristic of Chinese medicine and be polymer or low molecular compound; Because the mild action condition of enzyme, the thermo-labile and volatile component of part is kept in degradation process; Nontoxic, the no strong acid of Chinese drugs agentia through enzyme extraction, highly basic; Extraction yield and utilization ratio are all above more than 30% of conventional method.
Embodiment:
Example 1: Enzymatic Extraction Flos Lonicerae chlorogenic acid
One, 7 times of distilled water to Japanese Honeysuckle are added in the dried Japanese Honeysuckle fragmentation that fresh nothing is gone mouldy, and contain in the stainless steel fermentor tank.
Two, add 3 ‰ cellulases, 2 ‰ polygalacturonases, 32 ℃-45 ℃ of PH5.5～6.5 temperature, fermentation time 120 minutes.Aerobic stirs.Filter, filtrate is stand-by.
Three, adding distil water is equivalent to for the first time 70% in the filter residue, adds a-amylase, beta-amylase each 2 ‰, PH5.5～6.5,60 ℃-70 ℃ of temperature, 80 minutes time.Aerobic stirs.Filter, filtrate is stand-by.
Four, filter residue again adding distil water be equivalent to primaryly 70%, add neutral protease 3 ‰, PH7～7.5,38 ℃-45 ℃ of temperature, 60 minutes time.Aerobic stirs.Filter, filtrate is stand-by.
Five, above-mentioned three filtrates are mixed, and vacuum decompression concentrates, 60 ℃ of temperature.
Six, ethanol sedimentation, ethanol content 60%, 72 hours time, normal temperature.Filter residue precipitation twice, the smart filter of gained solution.Vacuum decompression reclaims ethanol.Can.Pasteurization.
Example 2: Poria fungus nucleome Enzymatic Extraction Pachymose
One, the Poria fungus nucleome that fresh nothing is gone mouldy is broken into 20 orders.Add 5 times of distilled water, contain in the stainless steel fermentor tank to the Poria fungus nucleome.
Two, add 5 ‰ cellulases, PH5.5～6.5,32 ℃-45 ℃ of temperature, fermentation time 120 minutes.Aerobic stirs.Filter, filtrate is stand-by.
Three, add 3 ‰ polygalacturonases, PH5.0-6.0,28 ℃-40 ℃ of temperature, 80 minutes time, aerobic stirs.Filter, filtrate is stand-by.
Four, add 3 ‰ cellulases, the same binomial of fermentation process.
Five, above-mentioned three filtrates are mixed, and vacuum decompression concentrates, 60 ℃ of temperature.
Six, freezing precipitation, 3 ℃ of temperature, 24 hours time.Smart filter.Can.Pasteurization.
Claims (2)
1, adopts the method for a series of enzyme extraction Flos Lonicerae chlorogenic acids, the steps include:
1. 7 times of distilled water to Japanese Honeysuckle are added in the dried Japanese Honeysuckle fragmentation that fresh nothing is gone mouldy, and contain in the stainless steel fermentor tank;
2. add 3 ‰ cellulases, 2 ‰ polygalacturonases, PH5.5～6.5,32 ℃～45 ℃ of temperature, fermentation time 120 minutes, aerobic stirring, filtration, filtrate are stand-by;
3. adding distil water in the filter residue is equivalent to for the first time 70%, add a amylase, B amylase each 2 ‰, PH5.5～6.5,60 ℃～70 ℃ of temperature, 80 minutes time, aerobic stirring, filtration, filtrate are stand-by;
4. filter residue again adding distil water be equivalent to primaryly 70%, add neutral protease 3 ‰, PH7～7.5,38 ℃～45 ℃ of temperature, 60 minutes time, aerobic stirring, filtration, filtrate are stand-by;
5. above-mentioned three filtrates are mixed, and vacuum decompression concentrates, 60 ℃ of temperature;
6. ethanol sedimentation, ethanol content 60%, 72 hour, normal temperature, filter residue precipitation twice, the smart filter of gained solution, vacuum decompression reclaims ethanol, can, pasteurization.
2, adopt the method for a series of enzyme extraction Poria fungus nucleome polysaccharide, the steps include:
1. the Poria fungus nucleome that fresh nothing is gone mouldy is broken into 20 orders, adds 5 times of distilled water to the Poria fungus nucleome, contains in the stainless steel fermentor tank;
2. add 5 ‰ cellulases, PH5.5～6.5,32 ℃～45 ℃ of temperature, fermentation time 120 minutes, aerobic stirring, filtration, filtrate are stand-by;
3. add 3 ‰ polygalacturonases, PH5.0～6.0,28 ℃～40 ℃ of temperature, fermentation time 80 minutes, aerobic stirring, filtration, filtrate are stand-by;
4. add 3 ‰ cellulases, PH5.5～6.5,32 ℃～45 ℃ of temperature, fermentation time 120 minutes, aerobic stirring, filtration, filtrate are stand-by;
5. above-mentioned three filtrates are mixed, vacuum decompression concentrates 60 ℃ of temperature;
6. freezing precipitation, 3 ℃ of temperature, 24 hours time, smart filter, can, pasteurization.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN93120161A CN1058293C (en) | 1993-12-08 | 1993-12-08 | Chinese herb medicine extraction by enzymolysis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN93120161A CN1058293C (en) | 1993-12-08 | 1993-12-08 | Chinese herb medicine extraction by enzymolysis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1103665A CN1103665A (en) | 1995-06-14 |
| CN1058293C true CN1058293C (en) | 2000-11-08 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN93120161A Expired - Fee Related CN1058293C (en) | 1993-12-08 | 1993-12-08 | Chinese herb medicine extraction by enzymolysis |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103330924B (en) * | 2013-06-07 | 2015-07-29 | 朱民生 | A kind of biological extraction process of active Chinese drug component element |
| CN103621906B (en) * | 2013-11-12 | 2015-06-10 | 安徽省葛根生产力促进中心有限公司 | Chufa and leonurus pseudomacranthus rice crust |
| CN103598325A (en) * | 2013-11-13 | 2014-02-26 | 王玉清 | Health-care poria cocos yoghourt and preparation method thereof |
| CN104257767B (en) * | 2014-09-19 | 2017-05-17 | 西藏藏真堂藏药产业有限公司 | Method for extracting active ingredients of rheum australe and application thereof |
| CN107412275A (en) * | 2017-05-11 | 2017-12-01 | 张新 | The preparation method of a variety of biology enzyme micro mist fermentation preparations |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1053361A (en) * | 1990-01-17 | 1991-07-31 | 大连生物化学制药厂 | Thromboembolism preventing blood fat reducing buccal tablet and production method thereof |
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1993
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1053361A (en) * | 1990-01-17 | 1991-07-31 | 大连生物化学制药厂 | Thromboembolism preventing blood fat reducing buccal tablet and production method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 中草药1981,12(11) 1981.11.30 王崇云等，栀子成分的研究 * |
| 中草药1981,12(11) 1981.11.30 王崇云等，栀子成分的研究;中草药1981,12(12) 1981.12.31 胡昌奇等，穿心莲中的新的二萜内酯类成分研究 * |
| 中草药1981,12(12) 1981.12.31 胡昌奇等，穿心莲中的新的二萜内酯类成分研究 * |
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