CN105816524A - Anti-inflammatory active cortex illicii extract as well as preparation method and application thereof - Google Patents
Anti-inflammatory active cortex illicii extract as well as preparation method and application thereof Download PDFInfo
- Publication number
- CN105816524A CN105816524A CN201610239314.6A CN201610239314A CN105816524A CN 105816524 A CN105816524 A CN 105816524A CN 201610239314 A CN201610239314 A CN 201610239314A CN 105816524 A CN105816524 A CN 105816524A
- Authority
- CN
- China
- Prior art keywords
- cortex illicii
- extract
- preparation
- illicii
- inflammatory activity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/57—Magnoliaceae (Magnolia family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
- A61K31/05—Phenols
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/075—Ethers or acetals
- A61K31/085—Ethers or acetals having an ether linkage to aromatic ring nuclear carbon
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Botany (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Alternative & Traditional Medicine (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses anti-inflammatory active cortex illicii extract as well as a preparation method and an application thereof. According to the preparation method of the anti-inflammatory active cortex illicii extract, the anti-inflammatory active cortex illicii extract is prepared by purifying crude cortex illicii extract according to any of the following methods a-c: a, performing silica-gel column chromatography on the crude cortex illicii extract, performing gradient elution by taking petroleum ether-ethyl acetate as an eluent, collecting an elution solution based on a volume ratio of petroleum ether to ethyl acetate of (5-15) to 1, and performing concentration and drying to obtain the anti-inflammatory active cortex illicii extract; b, performing C18 chromatographic column chromatography on the crude cortex illicii extract, performing gradient elution by taking methanol-water or ethanol-water as an eluent, collecting 80-90% by volume of an alcohol elution solution, and performing concentration and drying to obtain the anti-inflammatory active cortex illicii extract; and c, passing a macroporous resin column by the crude cortex illicii extract, performing gradient elution by taking methanol-water or ethanol-water as an eluent, collecting 90-100% by volume of an alcohol elution solution, and performing concentration and drying to obtain the anti-inflammatory active cortex illicii extract. The extract disclosed by the invention has a very good inhibition effect (remarkably superior to that of magnolol) for inflammations.
Description
Technical field
The present invention relates to the extraction of active component in plant, be specifically related to Cortex Illicii anti-inflammatory activity extract and
Its preparation method and application.
Background technology
Nitric oxide (nitric oxide, NO), as a kind of free radical, had both had second message,second messenger and neurotransmitter
Function, again can be with the pathology of the multiple diseases such as the numerator mediated inflammation of action effect, immunity and physiological process.
When immunocyte is stimulated by microbial endotoxins, inflammatory mediator etc., substantial amounts of induction type one can be generated
Nitric oxide synthase (iNOS), iNOS, by being catalyzed its substrate L-arginine, generates a large amount of NO and exempts from
Epidemic disease response, the NO of excess can cause tissue injury.In acute and chronic inflammation, NO is important
Pro-inflammatory cytokine.The experimental results shows that the joint fluid of rheumatisant and serum NO level are the highest
In normal person.NO serves not only as a kind of inflammatory mediator and directly take part in rheumatismal generation, can promote again it
Its inflammatory factor release, and then promote articular cartilage damage, increases the weight of that sb.'s illness took a turn for the worse.Reduce the generation of NO
Can be that arthritis provides protection.
Strong medicine Cortex Illicii is the dry bark of Illicium Cortex Illicii Illicium difengpi K.I.B et K.I.M,
Include kind for Chinese Pharmacopoeia, there is effect of expelling wind and removing dampness, promoting the circulation of QI to relieve pain.But up to now, have no
There is the relevant report of Cortex Illicii anti-inflammatory activity extract and preparation method thereof.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of Cortex Illicii anti-inflammatory activity extract and preparation side thereof
Method and application.
The preparation method of the Cortex Illicii anti-inflammatory activity extract that the present invention provides, comprises the following steps:
1) Cortex Illicii crude extract is obtained；
2) any one in Cortex Illicii crude extract a-c as follows is purified with prepared Cortex Illicii resist
Scorching activity extract；
Method a: by silica gel column chromatography on Cortex Illicii crude extract, with petroleum ether-ethyl acetate as eluant,
Gradient elution, collects VPetroleum ether: VEthyl acetateThe eluent of=5-15:1, concentrates, and is dried, obtains Cortex Illici difengpi
Skin anti-inflammatory activity extract；
Method b: by C18 column chromatography on Cortex Illicii crude extract, with methanol-water or alcohol-water as eluting
Agent, gradient elution, collected volume mark is 80-90% alcohol eluen, concentrates, and is dried, obtains Cortex Illicii
Anti-inflammatory activity extract；
Method c: by macroporous resin column chromatography on Cortex Illicii crude extract, with methanol-water or alcohol-water as eluting
Agent, gradient elution, collected volume mark is 90-100% alcohol eluen, concentrates, and is dried, obtains Cortex Illici difengpi
Skin anti-inflammatory activity extract.
The step 1 of above-mentioned preparation method) in, Cortex Illicii crude extract can the most commercially, it is possible to
To extract to obtain to Cortex Illicii by existing conventional method.Preferably, the application uses organic solvent
Cortex Illicii extracts to obtain Cortex Illicii crude extract, and wherein, described organic solvent is chloroform, second
Acetoacetic ester, volume fraction are 60-100% methanol or volume fraction is 60-100% ethanol；The mode extracted
And when number of times, extraction consumption and the extraction time of organic solvent the most same as the prior art, such as extracting mode
It can be extraction, heating extraction, reflux, extract, supersound extraction etc., it is preferred to use supersound extraction (work frequency
Rate is preferably 30～100KHz), the time of supersound extraction is usually 0.5～2h, and the consumption of Extraction solvent leads to
Extract on the basis of often adding 2-5L organic solvent by 1Kg Cortex Illicii.
The step 2 of above-mentioned preparation method) method a in, in order to reduce enter silicagel column crude extract in
Impurity, alleviates the burden of silicagel column, improves active component in gained Cortex Illicii anti-inflammatory activity extract simultaneously
Content, preferably by Cortex Illicii crude extract water dissolution, be extracted with ethyl acetate, collect organic facies (second
Acetoacetic ester phase), the organic facies collected is gone up silica gel column chromatography again.In the method, preferably collect VPetroleum ether:
VEthyl acetateThe eluent of=10:1.
The step 2 of above-mentioned preparation method) method b in, in order to reduce enter C18 chromatographic column crude extract
In impurity, alleviate the burden of C18 chromatographic column, improve in gained Cortex Illicii anti-inflammatory activity extract simultaneously
The content of active component, preferably by Cortex Illicii crude extract water dissolution, is extracted with ethyl acetate, and collects
Organic facies (ethyl acetate phase), goes up C18 column chromatography again by the organic facies collected.In order to subtract further
The burden of light C18 chromatographic column also improves the content of active component in product, preferably in the organic facies that will collect
C18 column chromatography is gone up again after first decolouring by MCI chromatographic column.
The step 2 of above-mentioned preparation method) method c in, the model of described macroporous resin be D101 or
HPD100。
The present invention also provides for the Cortex Illicii anti-inflammatory activity extract prepared by said method.
The present invention further provides the Cortex Illicii anti-inflammatory activity extract prepared by said method in preparation treatment
Application in the medicine of inflammation, specifically includes Cortex Illicii anti-inflammatory activity extract in preparation treatment by diformazan
Application in the medicine of the inflammation that benzene causes.
Compared with prior art, the present invention by Cortex Illicii crude extract through macroporous resin column, silicagel column or C18
To obtain Cortex Illicii anti-inflammatory activity extract (the wherein magnolol containing various active composition after column chromatography
Content >=25%, different Flos Carthami anise alcohol content >=5%, HPLC method), each active component association in extract
Same-action, has good inhibiting effect (its inhibitory action is substantially better than magnolol) to inflammation.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in further detail, to be more fully understood that the present invention's
Content, but the present invention is not limited to following example.
Embodiment 1
1) taking dry Cortex Illicii coarse powder 1.5Kg, adding volume fraction is that 80% ethanol 5L is heated to reflux
Extracting 1h, filter, medicinal residues repeat to extract 2 times, and united extraction liquid is evaporated to paste, obtains ground
Maple skin crude extract 182g；
2) Cortex Illicii crude extract 150g is taken, through C18 post (YMC*GEL ODS-A-HG) chromatography
Purification, with alcohol-water as eluant, gradient elution, collected volume mark is 80% ethanol-water solution
Eluent, concentrating under reduced pressure, it is dried, obtains the anti-inflammatory activity extract 8.2g of Cortex Illicii, wherein magnolol
Content is 36.8%, and the content of different Flos Carthami anise alcohol is 6.1%.
Embodiment 2
1) taking dry Cortex Illicii coarse powder 1.5Kg, add 3L chloroform extraction 18h, filter, medicinal residues repeat
Extract 2 times, united extraction liquid, be evaporated to paste, obtain Cortex Illicii crude extract 28g；
2) take Cortex Illicii crude extract 20g, upper silica gel column chromatography separating purification, with petroleum ether-ethyl acetate be
Eluant, gradient elution, collect VPetroleum ether: VEthyl acetate=10:1 eluent, concentrating under reduced pressure, it is dried,
To the anti-inflammatory activity extract 3.8g of Cortex Illicii, wherein the content of magnolol is 65.6%, and different Flos Carthami is anistree
The content of alcohol is 20.2%.
Embodiment 3
1) use raw material same as in Example 1 and slightly carry by the method acquisition Cortex Illicii that embodiment 1 is identical
Thing；
2) take above-mentioned Cortex Illicii crude extract 150g with water-dispersible, extract with ethyl acetate, collect ethyl acetate
Position by macroporous resin D101 purification on it, with ethanol-water system gradient elution, collected volume mark
It is 85% ethanol elution, concentrating under reduced pressure, it is dried, obtains the anti-inflammatory activity extract 10.6g of Cortex Illicii,
Wherein the content of magnolol is 26.2%, and the content of different Flos Carthami anise alcohol is 5.2%.
Embodiment 4
1) take dry Cortex Illicii coarse powder 1.5Kg, add 6L methanol supersound extraction 1h, filter, medicinal residues
Repeat to extract 1 time, united extraction liquid, be evaporated to paste, obtain Cortex Illicii crude extract 93.5g；
2) take Cortex Illicii crude extract 80g, with water-dispersible, extract with ethyl acetate, collect ethyl acetate extract
And isolated and purified by going up C18 column chromatography again after MCI post on it, with methanol-water as eluant, ladder
Degree eluting, collected volume mark is the eluent of 80-90% methanol-water solution, concentrating under reduced pressure, is dried,
Obtaining the anti-inflammatory activity extract 6.3g of Cortex Illicii, wherein the content of magnolol is 40.2%, and different Flos Carthami is anistree
The content of alcohol is 6.6%.
Embodiment 5
1) take dry Cortex Illicii coarse powder 1.5Kg, add 7.5L ethyl acetate supersound extraction 2h, filter,
Medicinal residues repeat to extract 2 times, and united extraction liquid is evaporated to paste, obtain Cortex Illicii crude extract 60.3g；
2) take above-mentioned Cortex Illicii and slightly carry silica gel column chromatography separating purification on 50g, with petroleum ether-ethyl acetate be
Eluant, gradient elution, collect VPetroleum ether: VEthyl acetate=10-15:1 eluent, concentrating under reduced pressure, it is dried,
Obtaining the anti-inflammatory activity extract 4.5g of Cortex Illicii, wherein the content of magnolol is 53.5%, different Flos Carthami eight
The content of angle alcohol is 17.2%.
Embodiment 6
1) use raw material the same as in Example 4 and slightly carry by the method acquisition Cortex Illicii implementing row 4 identical
Thing；
2) HPD100 macroporous resin column separating purification on above-mentioned Cortex Illicii crude extract 80g is taken, with methanol-water
System gradient elution, collected volume mark is 90% meoh eluate, concentrating under reduced pressure, is dried, obtains ground
The anti-inflammatory activity extract 10.1g of maple skin, wherein the content of magnolol is 25.5%, different Flos Carthami anise alcohol
Content is 5.1%.
Test example: the pharmacodynamic experiment result of Cortex Illicii anti-inflammatory activity extract
1, Cortex Illicii anti-inflammatory activity takes the RAW364.7 macrophage inflammation that LPS is induced by thing and magnolol
The impact that inflammation factor generates
Use NO content in Griess method detection RAW264.7 macrophage medium supernatant.Investigate
The RAW364.7 cell that LPS is induced by Cortex Illicii anti-inflammatory activity extract and monomeric compound produces NO
Suppression ratio.Result shows Cortex Illicii activity extract (method prepares as described in embodiment 1) and master thereof
Want monomeric compound magnolol and different Flos Carthami anise alcohol all can effectively suppress NO to generate, be shown in Table 1.
The RAW364.7 cell that LPS is induced by table 1 Cortex Illicii anti-inflammatory activity extract and monomeric compound thereof produces NO to be pressed down
Make and use
2, Cortex Illicii anti-inflammatory activity extract and magnolol xylol cause chmice acute inflammatory swelling to suppress
Effect study
KM male mice 70, is randomly divided into 7 groups by weight, if model group (blank group),
Positive controls (Western medicine: aspirin；Chinese patent medicine: Pa Fulin peony root total glycosides capsules), Cortex Illicii antiinflammatory
Activity extract high (H), in (M), low (L) dosage group, magnolol group.Gastric infusion, 1 time
/ d, continuous 3d, after last is administered 1h, all use liquid-transfering gun in forward and backward difference of auris dextra of 7 groups of mices
Uniform application dimethylbenzene 15 μ L, left ear is not coated with as comparison.Smearing rear 1h, cervical dislocation puts to death mice,
Cut left and right ear along auricle baseline, lay auricle at the same position of ears respectively with 7mm card punch, weigh,
Ear swelling degree between more each group, and calculate inhibitory rate of intumesce, the results are shown in Table 2.
Inhibitory rate of intumesce=(the average swelling of blank group-average swelling of administration group)/blank group is put down
All swelling × 100%.
Result shows: the high, medium and low equal xylol of dosage group of Cortex Illicii anti-inflammatory activity extract causes
Chmice acute inflammatory swelling has obvious inhibiting effect.Dosage group high, middle compares with blank group, difference
Property notable, point out it that inflammatory diseases is had therapeutical effect；Additionally, it is main in Cortex Illicii activity extract
Composition magnolol also xylol causes chmice acute inflammatory swelling to play inhibitory action.
Table 2 Cortex Illicii anti-inflammatory activity extract and the impact of magnolol xylol induced mice ear swelling
Compare with model group, * P < 0.05.
Claims (10)
1. the preparation method of Cortex Illicii anti-inflammatory activity extract, comprises the following steps:
1) Cortex Illicii crude extract is obtained；
2) any one in Cortex Illicii crude extract a-c as follows is purified with prepared Cortex Illicii
Anti-inflammatory activity extract；
Method a: by silica gel column chromatography on Cortex Illicii crude extract, with petroleum ether-ethyl acetate as eluant,
Gradient elution, collects VPetroleum ether: VEthyl acetateThe eluent of=5-15:1, concentrates, and is dried, obtains ground
Maple skin anti-inflammatory activity extract；
Method b: by C18 column chromatography on Cortex Illicii crude extract, with methanol-water or alcohol-water be
Eluant, gradient elution, collected volume mark is 80-90% alcohol eluen, concentrates, and is dried,
To Cortex Illicii anti-inflammatory activity extract；
Method c: by macroporous resin column chromatography on Cortex Illicii crude extract, with methanol-water or alcohol-water be
Eluant, gradient elution, collected volume mark is 90-100% alcohol eluen, concentrates, and is dried,
To Cortex Illicii anti-inflammatory activity extract.
Preparation method the most according to claim 1, it is characterised in that: step 1) in, use
Cortex Illicii is extracted to obtain Cortex Illicii crude extract by organic solvent.
Preparation method the most according to claim 2, it is characterised in that: described organic solvent
It is 60-100% methanol for chloroform, ethyl acetate, volume fraction or volume fraction is 60-100% ethanol.
4. according to the preparation method according to any one of claim 1-3, it is characterised in that: step
2), in method a, V is collectedPetroleum ether: VEthyl acetateThe eluent of=10:1.
5. according to the preparation method according to any one of claim 1-3, it is characterised in that: step
2) in method a, by Cortex Illicii crude extract water dissolution, it is extracted with ethyl acetate, collects organic facies
Go up silica gel column chromatography again.
6. according to the preparation method according to any one of claim 1-3, it is characterised in that: step
2) in method b, by Cortex Illicii crude extract water dissolution, it is extracted with ethyl acetate, collects organic facies
Go up C18 column chromatography again.
7. according to the preparation method according to any one of claim 1-3, it is characterised in that: step
2), in method b, described Cortex Illicii crude extract first uses MCI before formerly going up C18 column chromatography
Chromatographic column is decoloured.
8. according to the preparation method according to any one of claim 1-3, it is characterised in that: step 2)
In method c, the model of described macroporous resin is D101 or HPD100.
9. the Cortex Illicii anti-inflammatory activity that method according to any one of claim 1-8 prepares is extracted
Thing.
10. the Cortex Illicii anti-inflammatory activity extract described in claim 9 is at the medicine of preparation treatment inflammation
In application.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610239314.6A CN105816524B (en) | 2016-04-18 | 2016-04-18 | Cortex illicii anti-inflammatory active extract and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610239314.6A CN105816524B (en) | 2016-04-18 | 2016-04-18 | Cortex illicii anti-inflammatory active extract and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105816524A true CN105816524A (en) | 2016-08-03 |
| CN105816524B CN105816524B (en) | 2020-04-03 |
Family
ID=56526122
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610239314.6A Active CN105816524B (en) | 2016-04-18 | 2016-04-18 | Cortex illicii anti-inflammatory active extract and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105816524B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107467167A (en) * | 2017-06-22 | 2017-12-15 | 江南大学 | A kind of preparation method for significantly reducing the natural plant component of preservative dosage in meat products |
| CN107569535A (en) * | 2017-09-22 | 2018-01-12 | 右江民族医学院 | A kind of method for extracting anisetree bark active ingredient |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101250104A (en) * | 2008-03-19 | 2008-08-27 | 广西师范学院 | Method for extracting high-pure shikimic acid from scarlet octagonal fruit |
| CN102302555A (en) * | 2011-09-21 | 2012-01-04 | 中国人民解放军第二军医大学 | Chinese medicinal extract for treating urarthritis, as well as preparation method and application thereof |
| CN104586937A (en) * | 2013-11-01 | 2015-05-06 | 浙江泰康药业集团有限公司 | Preparation method of illicium plants injection |
| CN104926825A (en) * | 2015-05-07 | 2015-09-23 | 中国人民解放军第二军医大学 | Neolignan alkane derivatives promoting neurotrophic activity, and preparation method and application thereof |
| CN105334273A (en) * | 2015-11-26 | 2016-02-17 | 广西壮族自治区中国科学院广西植物研究所 | Quality control method of anisetree bark |
-
2016
- 2016-04-18 CN CN201610239314.6A patent/CN105816524B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101250104A (en) * | 2008-03-19 | 2008-08-27 | 广西师范学院 | Method for extracting high-pure shikimic acid from scarlet octagonal fruit |
| CN102302555A (en) * | 2011-09-21 | 2012-01-04 | 中国人民解放军第二军医大学 | Chinese medicinal extract for treating urarthritis, as well as preparation method and application thereof |
| CN104586937A (en) * | 2013-11-01 | 2015-05-06 | 浙江泰康药业集团有限公司 | Preparation method of illicium plants injection |
| CN104926825A (en) * | 2015-05-07 | 2015-09-23 | 中国人民解放军第二军医大学 | Neolignan alkane derivatives promoting neurotrophic activity, and preparation method and application thereof |
| CN105334273A (en) * | 2015-11-26 | 2016-02-17 | 广西壮族自治区中国科学院广西植物研究所 | Quality control method of anisetree bark |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107467167A (en) * | 2017-06-22 | 2017-12-15 | 江南大学 | A kind of preparation method for significantly reducing the natural plant component of preservative dosage in meat products |
| CN107569535A (en) * | 2017-09-22 | 2018-01-12 | 右江民族医学院 | A kind of method for extracting anisetree bark active ingredient |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105816524B (en) | 2020-04-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103304613B (en) | A kind of method of separation and purification 4 kinds of ucleosides chemical compositions from Snakegourd Peel | |
| CN104710502B (en) | A kind of method utilizing ionic liquid extract triptolide and B prime | |
| CN102924416A (en) | Method for separating and purifying monomeric compounds from ash bark | |
| CN105816524A (en) | Anti-inflammatory active cortex illicii extract as well as preparation method and application thereof | |
| CN102180938A (en) | Method for preparing capilliposide | |
| CN101638425B (en) | Method for extracting tripterine from celastrus orbiculatus root cortex | |
| CN106243120B (en) | The preparation of Sophora alopecuroide flavones Stilbene class extract and its application in cosmetics | |
| CN107929337A (en) | A kind of preparation method and application of Panax stipuleanatus Tsai et Feng anti-inflammatory active component | |
| CN101974005B (en) | Preparation method of high-content sesquiterpenoids tripterygium alkaloid | |
| CN104606184B (en) | The inflammatory applications and its extracting method of the not fragrant lactone of 7 (11) alkene 12,8 of 6 hydroxyl Airies in Radix Ligulariae Dentatae | |
| CN103520286A (en) | Extraction process of raw medicinal materials of exterior syndrome-relieving particles for children | |
| CN106349304A (en) | Method for preparing high-purity Urticaside and Urticalignans | |
| CN103304490A (en) | Method for separating and purifying five purine and pyrimidine bases from trichosanthes bark | |
| CN1903315B (en) | Use of active parts of fibrous root of Radix ophiopogonis | |
| CN105646424B (en) | A kind of method for extracting cyanidenon | |
| CN105348338B (en) | The method of separation paederosidic acid is extracted from Hainan Island Saprosma ternatum | |
| CN108836982A (en) | A kind of toad skin active component and preparation method thereof | |
| CN110759882B (en) | Dihydro agarofuran sesquiterpene and medical application thereof | |
| CN107266464A (en) | A kind of rhizoma alismatis decoction extract and its application | |
| CN102379889B (en) | Application of atemisia gmelinii extract in preparing drugs for preventing liver injuries | |
| CN104547148A (en) | Hedyotis diffusa extract used for preventing and treating senile dementia and preparation method of hedyotis diffusa extract | |
| CN105503993A (en) | Preparation method and application of nigella damascena saponin A | |
| CN108159195B (en) | Acer truncatum extract, preparation method and application thereof | |
| CN102532239A (en) | Method for extracting demethylzeylasteral from thunder god vine | |
| CN102188502B (en) | Extraction method and composition of common souliea rhizome total saponins with anti-tumor effect |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |