CN105815210A - Breeding method for maize inbred line high and low generations - Google Patents
Breeding method for maize inbred line high and low generations Download PDFInfo
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- CN105815210A CN105815210A CN201610171765.0A CN201610171765A CN105815210A CN 105815210 A CN105815210 A CN 105815210A CN 201610171765 A CN201610171765 A CN 201610171765A CN 105815210 A CN105815210 A CN 105815210A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- Genetics & Genomics (AREA)
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Abstract
The invention discloses a breeding method for maize inbred line high and low generations, relates to a method for obtaining improved varieties through a hybridization method, in particular to a method for achieving new maize inbred line cycle improvement, and aims at providing a method for breeding maize with the high quality and the good resistance. The breeding method for the maize inbred line high and low generations comprises the steps that a maize variety 5599 and a maize variety 6WC are hybridized to obtain a F1 generation; selfing is conducted to obtain a F2 generation; 5 ears of the F1 generation and 25 ears of the F2 generation are taken to be hybridized to obtain 125 F3 generation sister cross ears, the F3 generation is taken to be continuously selfed and screened, and the improved variety is obtained. According to the breeding method, it is guaranteed that the obtained hybridized seeds have the advantages of being high in quality and stress resistance, wide in adaptability and excellent in commodity property. The breeding method is used for the field of new crop variety breeding.
Description
Technical field
The present invention relates to a kind of method that method utilizing hybridization obtains improvement corn inbred line, after particularly relating to test out the combination that cooperation is higher, wherein one of parents there is also some little defects, and carrying out bicyclo-choosing in order to selfing line in the same group is the hybridizing method that improvement obtains corn variety.
Background technology
Owing to the corn variety that selection-breeding is excellent is difficult to there is persistence.Basic material is deficient and hereditary basis is narrow, and breeding difficulty has big breakthrough.Not, the less investment of medium-term and long-term maize breeding, that carries out is delayed, causes breeding basic material deficient and hereditary basis is narrow, reduces and improves hybrid vigor potentiality and resistance further, makes improved variety difficulty have big breakthrough for Genetic improvement, innovation.Along with the commercialization of breeding technique, the basic material scarcity ratio of corn breeding is the most prominent.So by Recycling breeding strategy, the most particularly important to kind of plasmagene superposition.
Summary of the invention
The technical problem to be solved in the present invention is to provide the method for the Semen Maydis of a kind of selection-breeding high-quality, better resistance, and corn variety received by the machine using the present invention can cultivate high-quality, better resistance, commodity good, meets the market demand.
The selection in corn inbred line of the present invention height generation, comprises the following steps:
One, corn variety 5599 is hybridized with 6WC, obtain hybridizing fruit ear F1 generation;
Two, taking 5 fringe fruit ears in F1 generation to keep sample, remaining continues selfing, obtains F2 generation;
Three, at F2 5 head progeny rows of generation, every head progeny row takes 5 fringe fruit ears, obtains 25 fruit ears;
Four, the 5 fringe fruit ears taking F1 generation in step 2 hybridize with the 25 fringe fruit ears in F2 generation in step 3, obtain 125 F3 generation height and hand over fruit ear for sisters;
Five, 125 fruit ears step 4 obtained combine resistance and quality trait screening individual plant, in continuous selfing 4-5 generation, i.e. obtain the stable corn inbred line of character 840;
In described step 5 for the resistance of screening and quality trait be the ripe phase 115-118 days, fruit ear line number 14-16 row, lodging resistance reach 3 grades, within disease-resistanting leaf spot 3, seed-producing rate reach more than 80%.
The selection difference from prior art in the corn inbred line height generation of the present invention is, the corn inbred line 840 using the present invention to select has the advantage that
1, economical character is excellent.The selfing line root system being bred as by the present invention is more flourishing, and fringe position is relatively low, and fruit ear seed-producing rate is high, mechanical planting quality better, and emasculation is simple, suitably works as female parent during hybrid seeding;Owing to combining the advantage between separate sources and type Semen Maydis, the selfing line of institute's selection-breeding has wide adaptability, strong stress resistance, is suitable for do mechanization operation.
2 and Huang changes class, the male parent resource of blue card class has good affinity, and assemble out the improved seeds such as the beautifulst 27.
Detailed description of the invention
Embodiment 1
The selection in the corn inbred line height generation of the present embodiment, comprises the following steps:
One, corn variety 5599 is hybridized with 6WC, obtain hybridizing fruit ear F1 generation;
Two, taking 5 fringe fruit ears in F1 generation to keep sample, remaining continues selfing, obtains F2 generation;
Three, at F2 5 head progeny rows of generation, every head progeny row takes 5 fringe fruit ears, obtains 25 fruit ears;
Four, the 5 fringe fruit ears taking F1 generation in step 2 hybridize with the 25 fringe fruit ears in F2 generation in step 3, obtain 125 F3 generation height and hand over fruit ear for sisters;
Five, 125 fruit ears step 4 obtained combine the ripe phase 115 days, fruit ear line number 16 row, lodging resistance reach 3 grades, within disease-resistanting leaf spot 3, seed-producing rate reach 80% selection individual plant carried out above, continuously selfing 4 generation, i.e. obtain the stable corn inbred line of character 840.
Embodiment 2
The selection in the corn inbred line height generation of the present embodiment, is with the difference of embodiment 1: the screening conditions in step 5 be the ripe phase 118 days, fruit ear line number 14 row, lodging resistance reach 3 grades, within disease-resistanting leaf spot 3, seed-producing rate reach more than 80%;Selfing 5 generation continuously.
Embodiment 3
The corn seed obtained in embodiment 1 and 2 precocious district period of duration northeastward is sowed in spring, about 115 days, seedling sheath purple, dark green leaf, early onset is extremely strong, average plant height 175 centimetres, 60 centimetres, fringe position, plant type is compact, and cane is tough and tensile, blade upper punch, tassel branch is medium, flower pesticide purple, filigree pink, average spike length 17 centimetres, thick 5.1 centimetres of fringe, tassel row number 12~14 row, cob is red, several 38 of average row grain, 100-grain weight 31 grams, fruit ear fringe handle is long, fruit ear letter type, and seed is orange-yellow, half Dens Equi type, seed-producing rate 84%.Variable rate technology: the diseases such as anti-northern and southern leaf blight, head smut, banded sclerotial blight, rough dwarf disease.Owing to combining the advantage between separate sources and type Semen Maydis, the selfing line of institute's selection-breeding has wide adaptability, strong stress resistance, is suitable for do mechanization operation.
Embodiment 4
The corn seed obtained in embodiment 1 and blue card class male parent LH52 hybridization are obtained honest beautiful 27, and the character of gained new varieties earnestly jade 27 is carried out observed and recorded.
2014 869 kilograms of adjacent comparison volume increase 8.0% of ratio of 20 average yield per mus in intra-company tests, in 20,19 increasings 1 subtract, a volume increase point rate 95%;Increase production 6.9% than class mean, 20 are all increased production, volume increase point rate 100%;335 meansigma methods volume increase 6.9% beautifulr than comparison, in 20,19 increasings 1 subtract, and volume increase point rate 95%, concrete yield is shown in Table 1.
Table honest beautiful 21 each testing site yield in 12014
2015 869 kilograms of adjacent comparison volume increase 7.2% of ratio of 20 average yield per mus in intra-company tests, in 20,19 increasings 1 subtract, a volume increase point rate 95%;Increasing production 6.7% than class mean, in 10,19 increasings 1 subtract, a volume increase point rate 95%;The beautifulst 335 volume increase 6.5% of ratio comparison, in 20,19 increasings 1 subtract, a volume increase point rate 95%.Each testing site economical character data summarization: the beautifulst 21 plant height 275cm, fringe position 115cm, the thick 5.4cm of spike length 20, the thick 2.9cm of axle, bald point meansigma methods 0cm, tassel row number 17. row, average 43 of row grain number, mass of 1000 kernel 400g, seed-producing rate 85.8%, dual spike rate are average 1.6%, fringe type tube type, grain type Dens Equi grain, grain color is orange-yellow, axle color red, and concrete yield is shown in Table 2.
Table honest beautiful 21 each testing site yield in 22015
The new varieties seed-producing rate that the corn seed obtained in embodiment 1 and the hybridization of G. eurycarpa male parent TX89 obtain is significantly higher than matched group.The corn inbred line that the present invention prepares and Huang change class, the male parent resource of blue card class has good affinity, can breed original seed by selfing or mixed friendship.
Embodiment described above is only to be described the preferred embodiment of the present invention; not the scope of the present invention is defined; on the premise of designing spirit without departing from the present invention; various deformation that technical scheme is made by those of ordinary skill in the art and improvement, all should fall in the protection domain that claims of the present invention determines.
Claims (1)
1. the selection in a corn inbred line height generation, it is characterised in that comprise the following steps:
One, corn variety 5599 is hybridized with 6WC, obtain hybridizing fruit ear F1 generation;
Two, taking 5 fringe fruit ears in F1 generation to keep sample, remaining continues selfing, obtains F2 generation;
Three, at F2 5 head progeny rows of generation, every head progeny row takes 5 fringe fruit ears, obtains 25 fruit ears;
Four, the 5 fringe fruit ears taking F1 generation in step 2 hybridize with the 25 fringe fruit ears in F2 generation in step 3, obtain 125 F3 generation height and hand over fruit ear for sisters;
Five, 125 fruit ears step 4 obtained combine resistance and quality trait screening individual plant, in continuous selfing 4-5 generation, i.e. obtain the corn inbred line that character is stable;
In described step 5 for the resistance of screening and quality trait be the ripe phase 115-118 days, fruit ear line number 14-16 row, lodging resistance reach 3 grades, within disease-resistanting leaf spot 3, seed-producing rate reach more than 80%.
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN106171965A (en) * | 2016-08-25 | 2016-12-07 | 通化市农业科学研究院 | A kind of super many tassel row numbers Maize DH Lines leads to the selection of 15D969 |
Citations (3)
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CN104938324A (en) * | 2015-06-03 | 2015-09-30 | 新疆农垦科学院 | Seed production method for new corn NO.15 as premature corn hybrid |
CN105028182A (en) * | 2015-06-23 | 2015-11-11 | 浙江省东阳玉米研究所 | Seed preparation method for waxy maize hybrids of Zhenuoyu 7 |
CN105230473A (en) * | 2015-09-01 | 2016-01-13 | 山东连发农业科技有限公司 | Creation method of salt-resistant corn |
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- 2016-03-24 CN CN201610171765.0A patent/CN105815210A/en active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN104938324A (en) * | 2015-06-03 | 2015-09-30 | 新疆农垦科学院 | Seed production method for new corn NO.15 as premature corn hybrid |
CN105028182A (en) * | 2015-06-23 | 2015-11-11 | 浙江省东阳玉米研究所 | Seed preparation method for waxy maize hybrids of Zhenuoyu 7 |
CN105230473A (en) * | 2015-09-01 | 2016-01-13 | 山东连发农业科技有限公司 | Creation method of salt-resistant corn |
Non-Patent Citations (1)
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Publication number | Priority date | Publication date | Assignee | Title |
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CN106171965A (en) * | 2016-08-25 | 2016-12-07 | 通化市农业科学研究院 | A kind of super many tassel row numbers Maize DH Lines leads to the selection of 15D969 |
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