CN105797213A - Electroactivity nano fiber support material and preparation method thereof - Google Patents
Electroactivity nano fiber support material and preparation method thereof Download PDFInfo
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- CN105797213A CN105797213A CN201610190554.1A CN201610190554A CN105797213A CN 105797213 A CN105797213 A CN 105797213A CN 201610190554 A CN201610190554 A CN 201610190554A CN 105797213 A CN105797213 A CN 105797213A
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- nano fiber
- ceramic particle
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/40—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material
- A61L27/44—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having a macromolecular matrix
- A61L27/446—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having a macromolecular matrix with other specific inorganic fillers other than those covered by A61L27/443 or A61L27/46
-
- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/40—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties
- D04H1/42—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties characterised by the use of certain kinds of fibres insofar as this use has no preponderant influence on the consolidation of the fleece
- D04H1/4382—Stretched reticular film fibres; Composite fibres; Mixed fibres; Ultrafine fibres; Fibres for artificial leather
-
- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
- D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
- D04H1/728—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/12—Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/02—Materials or treatment for tissue regeneration for reconstruction of bones; weight-bearing implants
Abstract
The invention relates to an electroactivity nano fiber support material which solves the technical problems that an existing material is not ideal in structure and the bone repair effect is expected to be further improved are solved.The material is composed of surface-modified ceramic particle filler and one or more of polymer base bodies including poly-L-lacticacid, polylactic acid and glycolic acid copolymers and polycaprolactone.A layer of sodium citrate wraps the surface of the surface-modified ceramic particle filler, and the electroactivity nano fiber support material is of a three-dimensional network structure.The invention meanwhile provides a preparation method of the electroactivity nano fiber support material.The electroactivity nano fiber support material can be widely used for the field of bone repair composite preparation.
Description
Technical field
The present invention relates to tissue regeneration and material for repairing and preparation method thereof, particularly to a kind of electricity
Active nano fiber scaffold material and preparation method thereof.
Background technology
Artificial bone repair materials is owing to wide material sources, performance are controlled and low cost, at bone renovating material
In occupy critical role.But, presently used artificial material still can not meet clinical demand,
Its main cause is that artificial material can not be as biological tissue, with physiological stress or biochemical stimulation
Make respective reaction, the most do not possess biological reactivity, make permanent replacement of human body hard tissue defect repair
Multiple theory receives challenge greatly.Therefore, change permanent replacement and repair idea, give full play to machine
Body self-regeneration potentiality, exploitation there is biological activity and biological reactivity material to become current human body hard
The Main Trends of The Development of tissue renovation material.
Osseous tissue forms the electricity environment relied on, and the electroactive Bionic Design for bone renovating material carries
Supply important evidence.Electroactive for making embedded material have, researcher is by conventional piezoelectric pottery metatitanic acid
Barium (BaTiO3) or potassium-sodium niobate (KNN) etc. be applied to technical field of biological material.But simple pottery material
There is the high shortcoming being difficult to processing of fragility in material, thus is usually combined into macromolecule polymer material
Type is used for Bone Defect Repari.
Such as, the Chinese invention patent of Publication No. CN 104208754A discloses a kind of piezoelectricity work
Property bone-repairing composite material and preparation method thereof, wherein, material is by the pottery with nucleocapsid structure
Particulate filler and polymeric matrix are constituted, and the ceramic particle uniform filling with nucleocapsid structure is dispersed in institute
State in polymeric matrix;The nucleome of the ceramic particle filler with nucleocapsid structure is ceramic particle, pottery
The Surface coating of porcelain granule has Coated with Organic Matter layer.
This composite forming method maintains good electrology characteristic, pliability and clinical operability.
But in terms of material structure, it is fine and close real wall-shaped material, maintain cell growth microenvironment with
And the three-dimensional net structure feature of the aspect such as nutrition transmission and natural extracellular matrix is not quite similar, by
The Bone Defect Repari effect that this causes also remains to be further improved.
Summary of the invention
The present invention is contemplated to solve that current material structure is to be improved, Bone Defect Repari effect needs into one
Walk perfect technical problem, it is provided that a kind of electroactive nano fiber scaffold with three-dimensional net structure
Material and preparation method thereof.
To this end, the present invention provides a kind of electroactive nano fiber scaffold material, material is by surface modification
Ceramic particle filler and Poly-L-lactic acid, Poly(D,L-lactide-co-glycolide, polycaprolactone in
One or more polymeric matrixs are constituted, ceramic particle filling surface one layer of lemon of cladding of surface modification
Lemon acid sodium, electroactive nano fiber scaffold material is three-dimensional net structure.
Preferably, the material of ceramic particle filler be Barium metatitanate., in barium strontium titanate and potassium-sodium niobate
One or more.
Preferably, electroactive nano fiber scaffold material is micro/nano fibrous membrane material, wherein said electricity
Active nano fibre diameter is 100nm~600nm, and the thickness of fibrous membrane is 50 μm~500 μm,
A diameter of 50nm~200nm of ceramic particle filler.
Preferably, in electroactive nano fiber scaffold material, the ceramic particle filler of surface modification accounts for
The mass percent of electroactive nano fiber scaffold material is 1wt.%~20wt.%, and remaining is polymerization
Thing matrix.
Meanwhile, the present invention provides the preparation method of a kind of electroactive nano fiber scaffold material, surface
The preparation process of modified ceramic particle filler uses aqua-solution method to complete;Polymeric matrix and surface
The composite molding process of modified ceramic particle filler is completed by method of electrostatic spinning.
Preferably, the preparation process of the ceramic particle filler of surface modification includes that sodium citrate solution is joined
System, nano-particle dispersion in sodium citrate solution include that ultrasonic agitation process, nano-particle are outstanding
The mixing of supernatant liquid and polymer solution and the electrostatic spinning process of mixed liquor.
Preferably, the method that the present invention provides, comprise the steps: that (1) weighs sodium citrate and puts
In 2ml organic solvent trifluoroethanol, it is configured to the sodium citrate solution that concentration is 0.02g/mL,
Take ceramic particle to be placed in sodium citrate solution, carry out at sonic oscillation, stirring at ambient temperature
Manage each 30min, alternately 3~5 times so that the ceramic particle filler of surface modification is in a solvent
Form homodisperse suspension;(2) weigh 1g polymer, add 8mL organic solvent trifluoro
Ethanol, stirring 3h~8h makes it be completely dissolved, obtains polymer solution;(3) by step (1)
Middle gained suspension adds in step (2) resulting polymers solution, and stirring 6h~12h makes surface
Modified ceramic particle uniform filling is dispersed in polymeric matrix, obtains mixed liquor;(4) will step
Suddenly, in (3) gained mixed liquor injects injector for medical purpose, syringe is placed in electrostatic spinning apparatus, quiet
The device that receives of Electrospun is the one in flat board, copper mesh or roller, and electrostatic potential is 10kV~20
KV, spinning flow velocity is 0.2mL/h~1.0mL/h, syringe needle and the distance received between device be 10cm~
30cm, makes solvent volatilize completely in gained nano fibrous membrane is placed in vacuum drying oven, obtains electricity and lives
Property nano fiber scaffold material.
The invention have the benefit that
(1) present invention is by carrying out surface modification to nano-ceramic particle, effectively reduces nanometer
The reunion of grain, improves the interface between ceramic particle and polymer, it is achieved that ceramic particle is poly-
Dispersed in compound matrix, improves the electric property of composite, is made up of nanofiber
Nano fiber scaffold material maintain good three-dimensional net structure characteristic.
(2) timbering material obtained by the present invention and skeletonization relevant cell have good affinity,
Osteoblastic adhesion and propagation can be promoted;After et al. Ke, visible material has good tissue phase
Capacitive.
(3) electroactive nano fiber scaffold material provided by the present invention is tied at macro property and microcosmic
Good performance it is respectively provided with, during Bone Defect Repari, for adhesion, the life of skeletonization relevant cell on structure
Long and differentiation provides microenvironment preferably;Additionally, this preparation method can make bone renovating material have
There is good biological reactivity, contribute to improving Bone Defect Repari effect further.
Accompanying drawing explanation
Fig. 1 is that the scanning electron microscope of nano fiber scaffold material described in the embodiment of the present invention 1 is shone
Sheet;
Fig. 2 is that barium titanate nano ceramic particle described in the embodiment of the present invention 1 divides in PLLA fiber
The transmission electron microscope photo of cloth;
Fig. 3 is the X-ray diffractogram of nano fiber scaffold material described in the embodiment of the present invention 1;
Fig. 4 is the electric property figure (nanometer of nano fiber scaffold material described in the embodiment of the present invention 1
The dielectric constant of fiber scaffold material is 1.15 ± 0.02);
Fig. 5 is the external osteoblasts cultivation 1 of nano fiber scaffold material described in the embodiment of the present invention 1
It electron scanning micrograph;
Fig. 6 is the internal reparation defect of skull 4 of nano fiber scaffold material described in the embodiment of the present invention 1
The light microscopic photo of all tissue slices;
Fig. 7 is the morphology observation after mesenchymal stem cells MSCs growth on two kinds of membrane materials 5 days
(A is BTO/P (VDF-TrFE) composite casting film;B is BTO/PLLA composite nano fiber
Film);
Fig. 8 is mesenchymal stem cells MSCs proliferation activity detection on two kinds of membrane materials
(*p<0.05)。
Detailed description of the invention
The invention provides a kind of electroactive nano fiber scaffold material and preparation method thereof, below knot
The present invention will be further described to close the drawings and specific embodiments.
Embodiment 1
(1) weighing sodium citrate to be placed in 2ml organic solvent trifluoroethanol, being configured to concentration is 0.02
The sodium citrate solution of g/mL, takes 0.07g BaTiO3Ceramic particle is placed in sodium citrate solution,
Carry out sonic oscillation, each 30min of stir process at ambient temperature, alternately 3 times so that table
The BaTiO that face is modified3Ceramic particle filler (BNP@sodium citrate) is formed in a solvent and uniformly divides
The suspension dissipated;
(2) weigh 1g polymer P LLA, add 8mL organic solvent trifluoroethanol, stir 6h
Make it be completely dissolved, obtain polymer PLLA solution;
(3) gained suspension in step (1) is added step (2) resulting polymers PLLA molten
In liquid, stirring 10h makes the BaTiO of surface modification3Ceramic particle filler (BNP@sodium citrate)
It is dispersed in polymer P LLA matrix, obtains mixed liquor;
(4) step (3) gained mixed liquor is injected in injector for medical purpose, syringe is placed in quiet
Electric spinning device, the reception device of electrostatic spinning is flat board, and electrostatic potential is 13kV, spinning flow velocity
It is 15cm for 0.8mL/h, syringe needle and the distance received between device.Gained nano fibrous membrane is placed in
Make solvent volatilize completely in vacuum drying oven, obtain a kind of electroactive nano fiber scaffold material.
Barium titanate nano pottery is contained by the electroactive nano fiber scaffold material of above step gained
Granule and polymer P LLA, it is micro/nano fibrous membrane material, and thickness is 200 μm, nanofiber
A diameter of 400nm.Nano-ceramic particle quality in electroactive nano fiber scaffold material contains
Amount is 7wt.%.
Embodiment 2
(1) weighing sodium citrate to be placed in 2ml organic solvent trifluoroethanol, being configured to concentration is 0.02
The sodium citrate solution of g/mL, takes 0.05g BaTiO3Ceramic particle is placed in sodium citrate solution,
Carry out sonic oscillation, each 30min of stir process at ambient temperature, alternately 4 times so that table
The BaTiO that face is modified3Ceramic particle filler (BNP@sodium citrate) is formed in a solvent and uniformly divides
The suspension dissipated;
(2) weigh 1g polymer P LLA, add 8mL organic solvent trifluoroethanol, stir 5h
Make it be completely dissolved, obtain polymer PLLA solution;
(3) gained suspension in step (1) is added step (2) resulting polymers PLLA molten
In liquid, stirring 12h makes the BaTiO of surface modification3Ceramic particle filler (BNP@sodium citrate)
It is dispersed in polymer P LLA matrix, obtains mixed liquor;
(4) step (3) gained mixed liquor is injected in injector for medical purpose, syringe is placed in quiet
Electric spinning device, the reception device of electrostatic spinning is flat board, and electrostatic potential is 15kV, spinning flow velocity
It is 16cm for 1.0mL/h, syringe needle and the distance received between device.Gained nano fibrous membrane is placed in
Make solvent volatilize completely in vacuum drying oven, obtain a kind of electroactive nano fiber scaffold material.
Barium titanate nano pottery is contained by the electroactive nano fiber scaffold material of above step gained
Granule and polymer P LLA, it is micro/nano fibrous membrane material, and thickness is 300 μm, nanofiber
A diameter of 300nm.Nano-ceramic particle quality in electroactive nano fiber scaffold material contains
Amount is 5wt.%.
Embodiment 3
(1) weighing sodium citrate to be placed in 2ml organic solvent trifluoroethanol, being configured to concentration is 0.02
The sodium citrate solution of g/mL, takes 0.01g BaTiO3Ceramic particle is placed in sodium citrate solution,
Carry out sonic oscillation, each 30min of stir process at ambient temperature, alternately 4 times so that table
The BaTiO that face is modified3Ceramic particle filler (BNP@sodium citrate) is formed in a solvent and uniformly divides
The suspension dissipated;
(2) weigh 1g polymer P LLA, add 8mL organic solvent trifluoroethanol, stir 8h
Make it be completely dissolved, obtain polymer PLLA solution;
(3) gained suspension in step (1) is added step (2) resulting polymers PLLA molten
In liquid, stirring 8h makes the BaTiO of surface modification3Ceramic particle filler (BNP@sodium citrate)
It is dispersed in polymer P LLA matrix, obtains mixed liquor;
(4) step (3) gained mixed liquor is injected in injector for medical purpose, syringe is placed in quiet
Electric spinning device, the reception device of electrostatic spinning is flat board, and electrostatic potential is 15kV, spinning flow velocity
It is 12cm for 1.0mL/h, syringe needle and the distance received between device.Gained nano fibrous membrane is placed in
Make solvent volatilize completely in vacuum drying oven, obtain a kind of electroactive nano fiber scaffold material.
Barium titanate nano pottery is contained by the electroactive nano fiber scaffold material of above step gained
Granule and polymer P LLA, it is micro/nano fibrous membrane material, and thickness is 150 μm, nanofiber
A diameter of 200nm.Nano-ceramic particle quality in electroactive nano fiber scaffold material contains
Amount is 1wt.%.
Embodiment 4
(1) weighing sodium citrate to be placed in 2ml organic solvent trifluoroethanol, being configured to concentration is 0.02
The sodium citrate solution of g/mL, takes 0.1g potassium sodium niobate ceramic granule and is placed in sodium citrate solution,
Carry out sonic oscillation, each 30min of stir process at ambient temperature, alternately 5 times so that table
The potassium sodium niobate ceramic granular filler (KNN@sodium citrate) of face modification is formed uniformly in a solvent
Scattered suspension;
(2) weigh 1g polymer P LLA, add 8mL organic solvent trifluoroethanol, stir 7h
Make it be completely dissolved, obtain polymer PLLA solution;
(3) gained suspension in step (1) is added step (2) resulting polymers PLLA molten
In liquid, stirring 12h makes potassium sodium niobate ceramic granular filler (the KNN@citric acid of surface modification
Sodium) it is dispersed in polymer P LLA matrix, obtain mixed liquor;
(4) step (3) gained mixed liquor is injected in injector for medical purpose, syringe is placed in quiet
Electric spinning device, the reception device of electrostatic spinning is copper mesh, and electrostatic potential is 18kV, spinning flow velocity
It is 18cm for 0.5mL/h, syringe needle and the distance received between device.Gained nano fibrous membrane is placed in
Make solvent volatilize completely in vacuum drying oven, obtain a kind of electroactive nano fiber scaffold material.
Contain potassium-sodium niobate nanometer by the electroactive nano fiber scaffold material of above step gained to make pottery
Porcelain granule and polymer P LLA, it is micro/nano fibrous membrane material, and thickness is 300 μm, Nanowire
A diameter of 450nm of dimension.Nano-ceramic particle quality in electroactive nano fiber scaffold material
Content is 10wt.%.
Embodiment 5
(1) weigh sodium citrate to be placed in 2ml organic solvent (such as trifluoroethanol), be configured to dense
Degree is the sodium citrate solution of 0.02g/mL, takes 0.2g barium-strontium titanate ceramic granule and is placed in citric acid
In sodium solution, carry out sonic oscillation, each 30min of stir process at ambient temperature, alternately 3
Secondary so that the barium-strontium titanate ceramic granular filler (BST@sodium citrate) of surface modification is in a solvent
Form homodisperse suspension;
(2) weigh 1g polymer P LLA, add 8mL organic solvent trifluoroethanol, stir 8h
Make it be completely dissolved, obtain polymer PLLA solution;
(3) gained suspension in step (1) is added step (2) resulting polymers PLLA molten
In liquid, stirring 12h makes the barium-strontium titanate ceramic granular filler (BST@sodium citrate) of surface modification
It is dispersed in polymer P LLA matrix, obtains mixed liquor;
(4) step (3) gained mixed liquor is injected in injector for medical purpose, syringe is placed in quiet
Electric spinning device, the reception device of electrostatic spinning is roller, and electrostatic potential is 18kV, spinning flow velocity
It is 20cm for 0.3mL/h, syringe needle and the distance received between device.Gained nano fibrous membrane is placed in
Make solvent volatilize completely in vacuum drying oven, obtain a kind of electroactive nano fiber scaffold material.
Contain barium strontium titanate nanometer by the electroactive nano fiber scaffold material of above step gained to make pottery
Porcelain granule and polymer P LLA, it is micro/nano fibrous membrane material, and thickness is 400 μm, Nanowire
A diameter of 500nm of dimension.Nano-ceramic particle quality in electroactive nano fiber scaffold material
Content is 20wt.%.
Comparative example 6
The electroactive nano fiber scaffold material that the present invention uses electrostatic spinning technique to prepare is being tied
In three-dimensional network spline structure on structure, the host material cultivated as cell, can be not only cell
Growth provides three dimensional matrix microenvironment, makes cell present three dimensional growth state, and cell can be grown into material
Space is internal also organically combines (as shown in Figure 7) with material, and the transmission of beneficially nutritional labeling,
Thus significantly improve cell proliferation and tissue repair.And for tradition reality wall two dimensional surface material,
Cell is showed only as flat growth conditions, and its proliferation activity is less than on nano fiber scaffold material
Osteoblast activity (as shown in Figure 8).
Claims (7)
1. an electroactive nano fiber scaffold material, is characterized in that described material is by surface modification
In ceramic particle filler and Poly-L-lactic acid, Poly(D,L-lactide-co-glycolide, polycaprolactone one
Planting or multiple polymers matrix is constituted, the ceramic particle filling surface of described surface modification is coated with one layer
Sodium citrate, described electroactive nano fiber scaffold material is three-dimensional net structure.
Electroactive nano fiber scaffold material the most according to claim 1, it is characterised in that institute
The material stating ceramic particle filler is for the one in Barium metatitanate., barium strontium titanate and potassium-sodium niobate or many
Kind.
Electroactive nano fiber scaffold material the most according to claim 1, it is characterised in that institute
Stating electroactive nano fiber scaffold material is micro/nano fibrous membrane material, wherein said electroactive Nanowire
Tieing up a diameter of 100nm~600nm, the thickness of fibrous membrane is 50 μm~500 μm, ceramic particle
A diameter of 50nm~200nm of filler.
Electroactive nano fiber scaffold material the most according to claim 1, it is characterised in that institute
Stating in electroactive nano fiber scaffold material, the ceramic particle filler of surface modification accounts for electroactive nanometer
The mass percent of fiber scaffold material is 1wt.%~20wt.%, and remaining is polymeric matrix.
5. the electroactive nano fiber scaffold as described in Claims 1 to 4 any one claim
The preparation method of material, it is characterised in that the preparation process of the ceramic particle filler of described surface modification
Employing aqua-solution method completes;Described polymeric matrix is compound with the ceramic particle filler of surface modification
Forming process is completed by method of electrostatic spinning.
The preparation method of electroactive nano fiber scaffold material the most according to claim 5, its
It is characterised by that the preparation process of the ceramic particle filler of described surface modification includes that sodium citrate solution is joined
System, nano-particle dispersion in sodium citrate solution include that ultrasonic agitation process, nano-particle are outstanding
The mixing of supernatant liquid and polymer solution and the electrostatic spinning process of mixed liquor.
7. according to the preparation method of the electroactive nano fiber scaffold material described in claim 5 or 6,
It is characterized in that comprising the steps:
(1) weighing sodium citrate to be placed in 2ml organic solvent trifluoroethanol, being configured to concentration is 0.02
The sodium citrate solution of g/mL, takes ceramic particle and is placed in sodium citrate solution, at ambient temperature
Carry out sonic oscillation, each 30min of stir process, alternately 3~5 times so that surface modification
Ceramic particle filler forms homodisperse suspension in a solvent;
(2) weigh 1g polymer, add 8mL organic solvent trifluoroethanol, stir 3h~8h
Make it be completely dissolved, obtain polymer solution;
(3) gained suspension in step (1) is added in step (2) resulting polymers solution,
Stirring 6h~12h makes the ceramic particle uniform filling of surface modification be dispersed in polymeric matrix,
Obtain mixed liquor;
(4) step (3) gained mixed liquor is injected in injector for medical purpose, syringe is placed in quiet
Electric spinning device, the device that receives of electrostatic spinning is the one in flat board, copper mesh or roller, electrostatic
Voltage is 10kV~20kV, and spinning flow velocity is 0.2mL/h~1.0mL/h, syringe needle and reception device
Between distance be 10cm~30cm, make solvent in gained nano fibrous membrane is placed in vacuum drying oven
Volatilize completely, obtain electroactive nano fiber scaffold material.
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