CN105777360A - Culture solution and culture method for grifola frondosa liquid strains - Google Patents
Culture solution and culture method for grifola frondosa liquid strains Download PDFInfo
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- CN105777360A CN105777360A CN201610188640.9A CN201610188640A CN105777360A CN 105777360 A CN105777360 A CN 105777360A CN 201610188640 A CN201610188640 A CN 201610188640A CN 105777360 A CN105777360 A CN 105777360A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
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Abstract
The invention belongs to the technical field of edible fungi cultivation and particularly relates to a culture solution and a culture method for grifola frondosa liquid strains. The culture solution for grifola frondosa liquid strains provided by the invention mainly is prepared from glucose, soybean meal, wheat bran, monopotassium phosphate, magnesium sulfate, a defoamer, a growth substance and water. The culture solution for grifola frondosa liquid strains provided by the invention is capable of quickening the growth speed of culture, improving the vigor and vitality of the strains, and shortening the fungus age and growth cycle of grifola frondosa; furthermore, the liquid strains cultured by the culture solution are healthy and strong in development, high in anti-bacterial and anti-virus capability, uniform in fungus age and uniform in growth, and is capable of comprehensively improving the quality and yield of the grifola frondosa liquid strains and lowering the production cost.
Description
Technical field
The invention belongs to fungus growing technique field, be specifically related to culture fluid and the breeding method of a kind of Grifola frondosa liquid spawn.
Background technology
Grifola frondosa (Grifdafrondosa) belongs to Basidiomycotina Hymenomycetes, Aphyllophorales Polyporus, has another name called hen of the woods, Semen Castaneae bacterium, thousand Buddhist bacterium, weight mushroom, Flos Nelumbinis mushroom, dance mushroom etc..The sporophore of Grifola frondosa, rich in the aminoacid of needed by human and vitamin, has higher nutritive value, and during its children, meat is soft, tender and crisp delicious, very popular.The sporophore of Grifola frondosa, possibly together with beta glucan, has the effect of prevention diabetes, antitumor and AIDS virus resisting, has higher medical value.
Owing in nature, the quantity of wild Grifola frondosa is few, and presenting downward trend year by year, its quantity can not meet far away the demand in market.At present, the commonly used female kind of artificial cultivation method of Grifola frondosa makes original seed, expands the solid cultivating method making cultigen again, but this solid cultivating method exists cycle length, the irregular shortcoming of cell age.The liquid spawn produced due to liquid fermentation can overcome the defect of solid culture, and liquid spawn is accessed also has when cultivating in solid medium that flowing is fast, good dispersion, rudiment point are many, send out bacterium rapidly, mycelia covers advantage early and replaces Grifola frondosa solid cultivating method gradually.
Chinese patent application 200910201986.8 discloses a kind of Grifola frondosa liquid spawn and the method by its cultivation Grifola frondosa, the culture fluid formula of described Grifola frondosa is made up of Rhizoma Solani tuber osi, glucose, Semen Maydis powder, wheat bran lixiviating solution and agar, and the raw material of the cultivating bacteria bag of its Grifola frondosa is become with local soil type by wood flour, cotton seed hulls, wheat bran, Semen Maydis powder, brown sugar, Gypsum Fibrosum.Use above-mentioned liquid culture fluid to cultivate Grifola frondosa and can accelerate the speed of growth of strain, shorten and send out the bacterium time, the significant fabrication cycle shortening Grifola frondosa, and adopt the bacterium bag hyphal development that liquid spawn makes healthy and strong, cell age is neat, the same period being easy to the later stage urges flower bud to process, and promotes that the industrialization of Grifola frondosa produces.
Chinese patent application 201310267656.5 discloses a kind of method of Grifola frondosa strain cultivation and high-yield culturing, and the composition of described slant medium is mainly by Rhizoma Solani tuber osi (peeling), Testa Tritici, glucose, KH2PO4、MgSO4, peptone, VB1Form with agar；The composition of described liquid seed culture medium is mainly by Rhizoma Solani tuber osi (peeling), glucose, peptone, KH2PO4、MgSO4Form with VB；The composition of described fermentation culture basal medium is mainly by KH2PO4, VB, glucose, peptone, MgSO4Composition.Use above-mentioned culture medium culturing Grifola frondosa can be greatly shortened the production of hybrid seeds time of strain, improve strain quality, reduce strain production cost, improve Fruitbody, there is significantly high promotional value.
But, adopt the Grifola frondosa liquid spawn that aforesaid liquid culture medium culturing obtains to hold easily infected virus in the process of cultivation, be particularly susceptible infection yellow humic virus, cause that large-scale rotting and aetiolation occurs in Grifola frondosa, have a strong impact on the yield of Grifola frondosa.
Summary of the invention
In order to solve the deficiency that in prior art, Grifola frondosa strain cultivation exists, it is an object of the invention to provide the culture fluid of the Grifola frondosa liquid spawn that a kind of production cost is low, growth cycle is short, strain is neat, strain coverage rate high and anti-bacteria and anti-virus ability is strong and breeding method, to solve drawbacks described above.
The invention provides the culture fluid of a kind of Grifola frondosa liquid spawn, including following component and parts by weight thereof:
Glucose 70-80 part, analysis for soybean powder 8-16 part, Testa Tritici 2-6 part, potassium dihydrogen phosphate 1-4 part, magnesium sulfate 1-5 part, defoamer 0.05-0.1 part, growth regulator 4-8 part and water 600-700 part.
Further, the culture fluid of described Grifola frondosa liquid spawn includes following component and parts by weight thereof:
Glucose 72-78 part, analysis for soybean powder 10-16 part, Testa Tritici 2-6 part, potassium dihydrogen phosphate 1-3 part, magnesium sulfate 1-3 part, defoamer 0.05-0.08 part, growth regulator 4-8 part and water 600-680 part.
Further, the culture fluid of described Grifola frondosa liquid spawn includes following component and parts by weight thereof:
Glucose 76 parts, analysis for soybean powder 12 parts, 4 parts of Testa Tritici, potassium dihydrogen phosphate 3 parts, 2 parts of magnesium sulfate, defoamer 0.06 part, growth regulator 6 parts and 640 parts of water.
Further, described defoamer is THIX-298 medicine defoamer.
Further, described growth regulator is made up of by weight 4-6: 1-3 gastrodine and propylene glycol alginate sodium sulfate.
Further, described growth regulator is made up of by weight 5: 2 gastrodine and propylene glycol alginate sodium sulfate.
It addition, present invention also offers the breeding method of a kind of Grifola frondosa liquid spawn, comprise the following steps:
The cultivation of S1 slant tube strain: the culture fluid of the Grifola frondosa liquid spawn described in claim 1 is loaded in test tube, then sterilizing 30-60min at test tube being placed in 120-125 DEG C, dry in 35-37 DEG C of incubator after cooling, after 2-3 days, Grifola frondosa pure culture is inoculated on the solid medium of slant tube, and cultivate 7-10 days under 20-22 DEG C of constant temperature, obtain slant tube strain；
The cultivation of S2 triangular flask strain: the culture fluid of the Grifola frondosa liquid spawn described in claim 1 is loaded in triangular flask, then sterilizing 30-60min at triangular flask being placed in 120-125 DEG C, the slant tube strain mycelia obtained by step S1 after cooling is separated into the square tiles that length is 5-6mm and is inoculated in triangular flask and cultivates, and cultivate 7-9 days under 20-22 DEG C of constant temperature, obtain triangular flask liquid spawn；
The cultivation of S3 fermentation tank strain: the culture fluid of the Grifola frondosa liquid spawn described in claim 1 is loaded in fermentation tank, after sterilizing, fermentation jar temperature is down to 22-24 DEG C, the triangular flask liquid-spawn inoculation obtained by step S2 is cultivated in fermentation tank, cultivate 7-10 days under 20-22 DEG C of constant temperature, obtain fermentor liquid strain；
The preparation of S4 culture bottle: culture medium for cultivating is loaded on porous pot, sterilizing 2-3h, the fermentor liquid strain obtained by step S3 after cooling is inoculated in porous pot and cultivates, and described culture medium for cultivating is mainly made up of corn cob 20-40%, wood flour 20-40%, rural area soil 10-20%, Semen Maydis powder 4-8%, wheat bran 14-20%, sucrose 0.5-2% and Gypsum Fibrosum 0.5-2%；
S5 mycelia culture: the porous pot obtained by step S4 is put in culturing room and cultivates after 20-30 days, put in mushroom producing room and cultivate 22-25 days, to obtain final product.
Further, described step S4 porous pot is three hole bottles, and bore dia is 12-14mm, and the water content control in described porous pot is at 63-65%, and pH value is 5.5-6.0.
Further, in described step S4, culture medium for cultivating is made up of corn cob 30%, wood flour 30%, rural area soil 15%, Semen Maydis powder 5%, wheat bran 18%, sucrose 1% and Gypsum Fibrosum 1%.
Further, in described step S5 culturing room, in the bottle of porous pot, temperature controls at 24-26 DEG C, CO in bottle2Content controls at 1500-3000ppm, and the humid control in described culturing room is at 65-75%.
Further, in described step S5, the temperature of mushroom producing room controls at 15-20 DEG C, CO2Content controls at 800-1000ppm, and humid control is at 85-99%.Wherein, described mycelia recovers the humid control of mushroom producing room in period at 97-99%, and the humid control of described mycelia mushroom producing room in squaring period is at 85-95%.
Gastrodine in growth regulator provided by the invention is also called Gastrodine, and its chemical name is: to methylol benzene-β-D pyranglucoside, its chemical formula is: C13H18O7, No. CAS is: 62499-27-8.Gastrodine is to extract from the dry root block of orchid Rhizoma Gastrodiae to obtain, and it has calm preferably and soporific function, and neurasthenia, insomnia, headache syndromes are had mitigation.The chemical name of propylene glycol alginate sodium sulfate is alginic acid the third fat sodium sulfovinate, and molecular formula is: C6H9O7Na, No. CAS is: 9005-38-3.Propylene glycol alginate sodium sulfate has anticoagulation, blood fat reducing, improves the effects such as microcirculation, is mainly used in ischemic cerebrovascular, is also used for hyperlipidemia, the disease of cardiovascular system is also had certain curative effect.
Wood flour in culture medium for cultivating provided by the invention is the wood flour of deciduous species, and the article No. of polyether-modified silicon defoaming agent is: THIX-298 medicine defoamer, is purchased from Yantai Thinking Finechem Technology Co., Ltd..
The growth regulator being made up of by certain weight ratio gastrodine and propylene glycol alginate sodium sulfate provided by the invention, the speed of growth of Grifola frondosa can be promoted, improve vigor and the vitality of Grifola frondosa liquid spawn, shorten sprout time, improve the yield of mycelia and extracellular polysaccharide, improve the bacterial strain adaptability to yeasting and follow-up planting environment.Find through test, use Grifola frondosa strain cultivation liquid provided by the invention to cultivate Grifola frondosa and can be greatly shortened the strain cycle of Grifola frondosa, its strain cycle is about 42-48 days, the Grifola frondosa absorption to nutrient substance can also be effectively promoted simultaneously, its biological transformation ratio is more than 58%, and the industrialization being more beneficial for Grifola frondosa produces.
Further, growth regulator provided by the invention can also improve antibacterial, the anti-virus ability of Grifola frondosa liquid spawn, find through test, use the Grifola frondosa liquid spawn that Grifola frondosa strain cultivation liquid provided by the invention is cultivated in the course of cultivation, the yellow humic disease disease of its sporophore sends out rate within 5%, the yield of Grifola frondosa can be greatly improved, reduce loss.
Grifola frondosa strain cultivation liquid provided by the invention can accelerate the speed of growth of strain, improves vigor and the vitality of strain, shortens cell age and the growth cycle of Grifola frondosa.And the liquid spawn that this culture fluid is cultivated grows stalwartness, antibacterial strong with anti-virus ability, cell age is neat, and growth uniformly, improves quality and the yield of Grifola frondosa liquid spawn, reduction production cost comprehensively.
Compared with prior art, technical scheme provided by the invention has the advantage that
(1) culture fluid of Grifola frondosa liquid spawn provided by the invention can accelerate the speed of growth of strain, improves vigor and the vitality of strain, shortens cell age and the growth cycle of Grifola frondosa；
(2) culture fluid of Grifola frondosa liquid spawn provided by the invention can improve the anti-bacteria and anti-virus ability of Grifola frondosa liquid spawn, especially yellow humic disease virus is had good resistant function, it is possible to effectively reduce loss；
(3) the breeding method cost of Grifola frondosa liquid spawn provided by the invention is low, and planting time is short, is conducive to the industrialization of this breeding method to produce.
Detailed description of the invention:
Below by way of the description of detailed description of the invention, the invention will be further described, but this is not limitation of the present invention, those skilled in the art's basic thought according to the present invention, various modifications may be made or improves, but without departing from the basic thought of the present invention, all within the scope of the present invention.
Embodiment 1,
The culture fluid of described Grifola frondosa liquid spawn includes following component and parts by weight thereof:
Glucose 70 parts, analysis for soybean powder 8 parts, 2 parts of Testa Tritici, potassium dihydrogen phosphate 2 parts, 2 parts of magnesium sulfate, defoamer 0.05 part, growth regulator 4 parts and 600 parts of water；Described growth regulator is made up of by weight 4: 3 gastrodine and propylene glycol alginate sodium sulfate.
The breeding method of described Grifola frondosa liquid spawn:
The cultivation of S1 slant tube strain: above-mentioned Grifola frondosa strain cultivation liquid is loaded in test tube, then test tube is placed in sterilizing 40min at 123 DEG C, dry in 36 DEG C of incubators after cooling, after 2 days, Grifola frondosa pure culture is inoculated on the solid medium in slant tube, and cultivate 10 days under 22 DEG C of constant temperatures, obtain slant tube strain；
The cultivation of S2 triangular flask strain: above-mentioned Grifola frondosa strain cultivation liquid is loaded in triangular flask, then triangular flask is placed in sterilizing 40min at 123 DEG C, the slant tube strain mycelia obtained by step S1 after cooling is separated into the square tiles that length is 5mm and is inoculated in triangular flask and cultivates, and cultivate 9 days under 22 DEG C of constant temperatures, obtain triangular flask liquid spawn；
The cultivation of S3 fermentation tank strain: above-mentioned Grifola frondosa strain cultivation liquid is loaded in fermentation tank, after sterilizing, fermentation jar temperature is down to 22 DEG C, the triangular flask liquid-spawn inoculation obtained by step S2 is cultivated in fermentation tank, cultivates 10 days, obtain fermentor liquid strain under 22 DEG C of constant temperatures；
The preparation of S4 culture bottle: culture medium for cultivating is loaded on porous pot, porous pot is three hole bottles, bore dia is 13mm, water content control in porous pot is 65%, pH value is 6.0, sterilizing 2h, the fermentor liquid strain obtained by step S3 after cooling is inoculated in porous pot and cultivates, and described culture medium for cultivating is made up of corn cob 30%, wood flour 30%, rural area soil 15%, analysis for soybean powder 5%, wheat bran 18%, sucrose 1% and Gypsum Fibrosum 1%；
S5 mycelia culture: the porous pot obtained by step S4 is put in culturing room and cultivates after 25 days, and in the bottle of described porous pot, temperature controls at 25 DEG C, CO in bottle2Control the humid control in 2000ppm, described culturing room 70%；Putting into mushroom producing room to cultivate 25 days, the temperature of described mushroom producing room controls at 18 DEG C, CO2Content controls at 800ppm, and described mycelia recovers the humid control of mushroom producing room in period 98%, and the humid control of described mycelia mushroom producing room in squaring period, 90%, to obtain final product.
Embodiment 2,
The culture fluid of described Grifola frondosa liquid spawn includes following component and parts by weight thereof:
Glucose 76 parts, analysis for soybean powder 12 parts, 4 parts of Testa Tritici, potassium dihydrogen phosphate 3 parts, 2 parts of magnesium sulfate, defoamer 0.06 part, growth regulator 6 parts and 640 parts of water；Described growth regulator is made up of by weight 5: 2 gastrodine and propylene glycol alginate sodium sulfate.
The breeding method of described Grifola frondosa liquid spawn is similar to Example 1.
Embodiment 3,
The culture fluid of described Grifola frondosa liquid spawn includes following component and parts by weight thereof:
Glucose 80 parts, analysis for soybean powder 16 parts, 6 parts of Testa Tritici, potassium dihydrogen phosphate 4 parts, 5 parts of magnesium sulfate, defoamer 0.1 part, growth regulator 8 parts and 700 parts of water；Described growth regulator is made up of by weight 6: 1 gastrodine and propylene glycol alginate sodium sulfate.
The breeding method of described Grifola frondosa liquid spawn is similar to Example 1.
Comparative example 1,
The culture fluid of described Grifola frondosa liquid spawn includes following component and parts by weight thereof:
Glucose 76 parts, analysis for soybean powder 12 parts, 4 parts of Testa Tritici, potassium dihydrogen phosphate 3 parts, 2 parts of magnesium sulfate, defoamer 0.06 part, growth regulator 6 parts and 640 parts of water；Described growth regulator is gastrodine.
The breeding method of described Grifola frondosa liquid spawn is similar to Example 1.
Being distinctive in that with embodiment 2, growth regulator is gastrodine.
Comparative example 2,
The culture fluid of described Grifola frondosa liquid spawn includes following component and parts by weight thereof:
Glucose 76 parts, analysis for soybean powder 12 parts, 4 parts of Testa Tritici, potassium dihydrogen phosphate 3 parts, 2 parts of magnesium sulfate, defoamer 0.06 part, growth regulator 6 parts and 640 parts of water；Described growth regulator is propylene glycol alginate sodium sulfate.
The breeding method of described Grifola frondosa liquid spawn is similar to Example 1.
Being distinctive in that with embodiment 2, growth regulator is propylene glycol alginate sodium sulfate.
Comparative example 3,
The culture fluid of described Grifola frondosa liquid spawn includes following component and parts by weight thereof:
Glucose 76 parts, analysis for soybean powder 12 parts, 4 parts of Testa Tritici, potassium dihydrogen phosphate 3 parts, 2 parts of magnesium sulfate, defoamer 0.06 part, growth regulator 6 parts and 640 parts of water；Described growth regulator is made up of by weight 1: 1 gastrodine and propylene glycol alginate sodium sulfate.
The breeding method of described Grifola frondosa liquid spawn is similar to Example 1.
Being distinctive in that with embodiment 2, in growth regulator, the weight ratio of gastrodine and propylene glycol alginate sodium sulfate is 1: 1.
Test example one, Grifola frondosa breeding strain periodic test
1, test material: the Grifola frondosa strain cultivation liquid of embodiment 1, embodiment 2, embodiment 3, comparative example 1, comparative example 2 and comparative example 3 preparation.
2, test method:
By embodiment 1, embodiment 2, embodiment 3, comparative example 1, the Grifola frondosa strain cultivation liquid of comparative example 2 and comparative example 3 preparation, it is respectively set to embodiment 1 group, embodiment 2 groups, embodiment 3 groups, comparative example 1 group, comparative example 2 groups and comparative example 3 groups, the Grifola frondosa strain cultivation liquid that wherein prepared by the embodiment 2 to be not added with growth regulator is as a control group, the culture fluid of difference group is cultivated under identical conditions Grifola frondosa strain, observe the slant tube strain of Grifola frondosa, triangular flask strain, the spawn culture cycle of fermentation tank strain, and record porous pot and cover with the time of mycelia, additionally calculate the biological transformation ratio of Grifola frondosa, biological transformation ratio (%)=sporophore fresh goods yield (g)/compost dry weight (g) × 100%.
3, result of the test
Result of the test is as shown in Table 1 and Table 2.
The cultivation cycle of table 1 Grifola frondosa liquid spawn
The biological transformation ratio of table 2 Grifola frondosa liquid spawn
| Sporophore fresh goods yield (g) | Planting material dry weight (g) | Biological transformation ratio (%) | |
| Matched group | 777.60 | 2250 | 34.56 |
| Embodiment 1 group | 1310.63 | 2250 | 58.25 |
| Embodiment 2 groups | 1397.25 | 2250 | 62.10 |
| Embodiment 3 groups | 1347.30 | 2250 | 59.88 |
| Comparative example 1 group | 871.20 | 2250 | 38.72 |
| Comparative example 2 groups | 820.13 | 2250 | 36.45 |
| Comparative example 3 groups | 955.13 | 2250 | 42.45 |
From above-mentioned table 1 and table 2, using the strain cycle that the embodiment 1-3 Grifola frondosa strain cultivation liquid prepared cultivates Grifola frondosa strain is about 42-48 days, biological transformation ratio is more than 58%, and the strain cycle that the Grifola frondosa strain cultivation liquid of comparative example 1 and comparative example 2 preparation cultivates Grifola frondosa strain is more than 60 days, biological transformation ratio is less than 40%, illustrate provided by the invention to be shortened the cultivation period of Grifola frondosa by ephedrine and propylene glycol alginate sodium sulfate by the growth regulator that certain weight ratio forms, improve the biological transformation ratio of Grifola frondosa liquid spawn, it is more beneficial for the production of industrialization and scale.
Test example two, Grifola frondosa liquid spawn anti-yellowing maize ear rot poison test
1, test material: the Grifola frondosa strain cultivation liquid of embodiment 1, embodiment 2, embodiment 3, comparative example 1, comparative example 2 and comparative example 3 preparation.
2, test method: by embodiment 1, embodiment 2, embodiment 3, comparative example 1, the Grifola frondosa strain cultivation liquid of comparative example 2 and comparative example 3 preparation, it is respectively set to embodiment 1 group, embodiment 2 groups, embodiment 3 groups, comparative example 1 group, comparative example 2 groups and comparative example 3 groups, the Grifola frondosa strain cultivation liquid that wherein prepared by the embodiment 2 to be not added with growth regulator is as a control group, the culture fluid of difference group is cultivated under identical conditions Grifola frondosa strain, observe Grifola Frondosa sporophore scab situation, and calculate Grifola Frondosa sporophore yellow humic disease incidence rate, wherein rotting occurs in Grifola Frondosa sporophore, atrophy, the phenomenon becoming beans shape is evaluated as occurs that yellow humic is sick, Grifola Frondosa sporophore yellow humic disease incidence rate=Grifola Frondosa sporophore scab number/all sporophore is total.
3, result of the test:
Result of the test is as shown in table 3.
Table 3 Grifola Frondosa sporophore yellow humic is sick, and a situation arises
| Grifola Frondosa sporophore yellow humic disease incidence rate (%) | |
| Matched group | 34.35 |
| Embodiment 1 group | 3.30 |
| Embodiment 2 groups | 3.44 |
| Embodiment 3 groups | 3.52 |
| Comparative example 1 group | 22.24 |
| Comparative example 2 groups | 28.76 |
| Comparative example 3 groups | 15.22 |
As shown in Table 3; use the Grifola frondosa liquid spawn incidence rate that yellow humic is sick in cultivating process that the embodiment of the present invention 1-3 Grifola frondosa strain cultivation liquid prepared is cultivated within 5%; and use the Grifola frondosa liquid spawn incidence rate that yellow humic is sick in cultivating process that the Grifola frondosa strain cultivation liquid of comparative example 1 and comparative example 2 preparation is cultivated more than 20%; illustrate provided by the invention to be improved the anti-virus ability of Grifola frondosa by ephedrine and propylene glycol alginate sodium sulfate by the growth regulator that certain weight ratio forms, be more beneficial for the production of industrialization and scale.
Claims (10)
1. the culture fluid of a Grifola frondosa liquid spawn, it is characterised in that include following component and parts by weight thereof:
Glucose 70-80 part, analysis for soybean powder 8-16 part, Testa Tritici 2-6 part, potassium dihydrogen phosphate 1-4 part, magnesium sulfate 1-5 part, defoamer 0.05-0.1 part, growth regulator 4-8 part and water 600-700 part.
2. the culture fluid of Grifola frondosa liquid spawn as claimed in claim 1, it is characterized in that, including following component and parts by weight thereof: glucose 76 parts, analysis for soybean powder 12 parts, 4 parts of Testa Tritici, potassium dihydrogen phosphate 3 parts, 2 parts of magnesium sulfate, defoamer 0.06 part, growth regulator 6 parts and 640 parts of water.
3. the culture fluid of Grifola frondosa liquid spawn as claimed in claim 1 or 2, it is characterised in that described defoamer is polyether-modified silicon defoaming agent.
4. the culture fluid of Grifola frondosa liquid spawn as claimed in claim 1 or 2, it is characterised in that described growth regulator is made up of by weight 4-6: 1-3 gastrodine and propylene glycol alginate sodium sulfate.
5. the culture fluid of Grifola frondosa liquid spawn as claimed in claim 4, it is characterised in that described growth regulator is made up of by weight 5: 2 gastrodine and propylene glycol alginate sodium sulfate.
6. the breeding method of a Grifola frondosa liquid spawn, it is characterised in that comprise the following steps:
The cultivation of S1 slant tube strain: the culture fluid of the Grifola frondosa liquid spawn described in claim 1 is loaded in test tube, then sterilizing 30-60min at test tube being placed in 120-125 DEG C, dry in 35-37 DEG C of incubator after cooling, after 2-3 days, Grifola frondosa pure culture is inoculated on the solid medium of slant tube, and cultivate 7-10 days under 20-22 DEG C of constant temperature, obtain slant tube strain；
The cultivation of S2 triangular flask strain: the culture fluid of the Grifola frondosa liquid spawn described in claim 1 is loaded in triangular flask, then sterilizing 30-60min at triangular flask being placed in 120-125 DEG C, the slant tube strain mycelia obtained by step S1 after cooling is separated into the square tiles that length is 5-6mm and is inoculated in triangular flask and cultivates, and cultivate 7-9 days under 20-22 DEG C of constant temperature, obtain triangular flask liquid spawn；
The cultivation of S3 fermentation tank strain: the culture fluid of the Grifola frondosa liquid spawn described in claim 1 is loaded in fermentation tank, after sterilizing, fermentation jar temperature is down to 22-24 DEG C, the triangular flask liquid-spawn inoculation obtained by step S2 is cultivated in fermentation tank, cultivate 7-10 days under 20-22 DEG C of constant temperature, obtain fermentor liquid strain；
The preparation of S4 culture bottle: culture medium for cultivating is loaded on porous pot, sterilizing 2-3h, the fermentor liquid strain obtained by step S3 after cooling is inoculated in porous pot and cultivates, and described culture medium for cultivating is mainly made up of corn cob 20-40%, wood flour 20-40%, rural area soil 10-20%, Semen Maydis powder 4-8%, wheat bran 14-20%, sucrose 0.5-2% and Gypsum Fibrosum 0.5-2%；
S5 mycelia culture: the porous pot obtained by step S4 is put in culturing room and cultivates after 20-30 days, put in mushroom producing room and cultivate 22-25 days, to obtain final product.
7. the breeding method of Grifola frondosa liquid spawn as claimed in claim 6, it is characterised in that described step S4 porous pot is three hole bottles, and bore dia is 12-14mm, and the water content control in described porous pot is at 63-65%, and pH value is 5.5-6.0.
8. the breeding method of Grifola frondosa liquid spawn as claimed in claim 6, it is characterised in that in described step S4, culture medium for cultivating is made up of corn cob 30%, wood flour 30%, rural area soil 15%, Semen Maydis powder 5%, wheat bran 18%, sucrose 1% and Gypsum Fibrosum 1%.
9. the breeding method of Grifola frondosa liquid spawn as claimed in claim 6, it is characterised in that in described step S5 culturing room, in the bottle of porous pot, temperature controls at 24-26 DEG C, CO in bottle2Content controls at 1500-3000ppm, and the humid control in described culturing room is at 65-75%.
10. the breeding method of Grifola frondosa liquid spawn as claimed in claim 6, it is characterised in that in described step S5, the temperature of mushroom producing room controls at 15-20 DEG C, CO2Content controls at 800-1000ppm, and humid control is at 85-99%.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610188640.9A CN105777360B (en) | 2016-03-28 | 2016-03-28 | A kind of culture solution and breeding method of grifola frondosus liquid spawn |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610188640.9A CN105777360B (en) | 2016-03-28 | 2016-03-28 | A kind of culture solution and breeding method of grifola frondosus liquid spawn |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105777360A true CN105777360A (en) | 2016-07-20 |
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| CN111296177A (en) * | 2020-03-13 | 2020-06-19 | 江苏华绿生物科技股份有限公司 | Liquid strain culture medium applied to industrial cultivation of grifola frondosa and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1398979A (en) * | 2002-08-16 | 2003-02-26 | 维京仲华（上海）生物医药科技有限公司 | Fermentation process of ash tree flower and production process of its polyglycopeptide |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1398979A (en) * | 2002-08-16 | 2003-02-26 | 维京仲华（上海）生物医药科技有限公司 | Fermentation process of ash tree flower and production process of its polyglycopeptide |
Non-Patent Citations (2)
| Title |
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| 赵亮: "以中药为基质的灰树花发酵工艺条件的研究", 《中国优秀硕士学位论文全文数据库农业科技辑》 * |
| 黄涛华: "灰树花高产栽培技术", 《福建农业》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111296177A (en) * | 2020-03-13 | 2020-06-19 | 江苏华绿生物科技股份有限公司 | Liquid strain culture medium applied to industrial cultivation of grifola frondosa and preparation method thereof |
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