CN105772127B - A kind of biochip hybridizing device - Google Patents
A kind of biochip hybridizing device Download PDFInfo
- Publication number
- CN105772127B CN105772127B CN201610273330.7A CN201610273330A CN105772127B CN 105772127 B CN105772127 B CN 105772127B CN 201610273330 A CN201610273330 A CN 201610273330A CN 105772127 B CN105772127 B CN 105772127B
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- box
- hybridizing
- biochip
- lid
- box body
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
- B01L3/50855—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates using modular assemblies of strips or of individual wells
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
- B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0819—Microarrays; Biochips
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/10—Means to control humidity and/or other gases
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
Abstract
The invention discloses a kind of biochip hybridizing device, feature is to include hybridization wet box and hybridizing box, being horizontally disposed with hybridization wet box has multiple storing chambers for being used to put hybridizing box, hybridizing box includes hybridizing box box body and hybridizing box lid, chip placement cavity is provided with hybridizing box box body, multiple watch windows have been arranged side by side in hybridizing box lid outer face, the edge of watch window is provided with a well, hybridizing box lid inner face, which is provided with, to be used for the securing chip positioning block in biochip position, the position of chip positioning block is corresponding with the position of chip placement cavity, one end of hybridizing box box body and hybridizing box lid passes through gemel connection, hybridizing box box body is connected with the other end of hybridizing box lid by latch components.Advantage is simple in construction, can be prevented effectively from the generation of slide plate phenomenon, it is ensured that the success rate of hybridization, and by hybridizing hybridization offer one constant hybridization space of the wet box to biochip, the auxiliary equipment extra without other saves cost.
Description
Technical field
The present invention relates to biochip technology field, more particularly, to a kind of biochip hybridizing device.
Background technology
With the fast development of biotechnology and related discipline, the application of biochip is more and more extensive.Biochip
Using being to be hybridized the biological specimen of itself and mark, the number of the strong and weak judgement sample target by detecting hybridization signal
Amount.
Chinese invention patent 200610114412.3 discloses a kind of biochip hybridizing box, including base, upper lid, clip slot
And bracket, biochip when in use, is seated on bracket, then substrate is placed on biochip by the hybridizing box first,
Biochip and substrate be separation, when sample is added into conversion zone using micropipettor, due to injection aperture compared with
Small, the suction nozzle of conventional pipettor is easy to drive substrate to be shifted over, so that substrate is taken away into biochip surface, that is, is easy to production
The phenomenon of raw slide plate is so as to cause hybridization to fail, and in order to avoid the generation of slide plate phenomenon, some operating personnel can use minimum suction
The micropipettor of head, is repeatedly injected, this can cause bubble to enter conversion zone to reagent, so as to increase the failure of hybridization
Probability；In addition, it is necessary to place it in the water of the constant environment of temperature such as constant temperature, perseverance after biochip and substrate installation
In incubator or hybrid heater, now need to cover lid, the seat sealing of upper lid and bottom is fixed by clip slot, then will entirely be hybridized
Box is placed in the water of constant temperature or other corresponding equipment, and above-mentioned sealing fixed form is complicated, and clip slot sealing is fixed
When the sample and reagent that can produce in certain extruding force, chip conversion zone be possible to be extruded by the effect of the extruding force
To outside conversion zone, so as to influence the reaction of biochip；The space that the hybridizing box of said structure takes simultaneously is larger, production
Cost is higher；Other constant temperature generally requires by extra instrument to complete, and this causes experimental cost higher.
The content of the invention
It is low and portable that the technical problems to be solved by the invention are to provide a kind of simple in construction, easy for operation, cost
Biochip hybridizing device, the biochip hybridizing device can be prevented effectively from slide plate phenomenon, also once can carry out multi-disc simultaneously
Hybridization, can also provide the hybridization environment of constant temperature and constant humidity by itself, and hybridization success rate is high.
The present invention solve the technical scheme that is used of above-mentioned technical problem for：
A kind of biochip hybridizing device, it is characterised in that including hybridizing in wet box and hybridizing box, described hybridization wet box
Horizontally disposed to have multiple storing chambers for being used to put described hybridizing box, described hybridizing box includes hybridizing box box body and hybridizing box
The chip placement cavity for putting biochip, described hybridizing box lid are provided with lid, described hybridizing box box body
Multiple watch windows to lower recess are arranged side by side in outer face, and the edge of described watch window is provided with a up/down perforation
Well, described hybridizing box lid inner face is provided with for by the described securing chip positioning in biochip position
Block, the position of described chip positioning block is corresponding with the position of described chip placement cavity, described hybridizing box box body with
One end of described hybridizing box lid is by gemel connection, the other end of described hybridizing box box body and described hybridizing box lid
Connected by latch components.
The bottom face of described watch window uses transparent material, is put into described chip when described biochip and puts
When putting in cavity, the sample area of the described biochip surface of described watch window alignment, the bottom of described watch window
Gap is provided between face and the upper surface of described biochip, described gap forms chip conversion zone, when described
When hybridizing box lid lid is set on described hybridizing box box body, the lower surface of described chip positioning block and described biochip
Upper surface be brought into close contact.Watch window bottom face uses transparent material, when biochip is put into chip placement cavity,
Sample area on biochip can accurately be placed in the lower section of the watch window by operator through the transparent watch window, just
Chip conversion zone is accurately entered by well in the solution containing hybridization composition, is connect with the sample in biochip surface
Touch, complete hybridization, it is ensured that the success rate of hybridization；The lower surface of chip positioning block and the upper surface of biochip are closely pasted simultaneously
Close, it is ensured that the stability of biochip positioning.
The tank for holding liquid is additionally provided with described hybridizing box box body.It can inject a certain amount of in the tank
Pure water, the hybridization to biochip provides the hybridization environment of a moistening.
Described tank is two, and the tank described in two is respectively symmetrically provided with the two of described chip placement cavity
Side.Tank is two, and two tanks are symmetrically arranged at the both sides of chip placement cavity, inject a certain amount of in the tank
Pure water so that the humidity on chip placement cavity both sides is able to unanimously.
Described gap is 0.2-0.5mm.
Described latch components include the lock seat being arranged on described hybridizing box lid, the described lower end for latching seat
Face, which is provided with lock groove, described hybridizing box box body, is provided with lock that is corresponding with described lock groove location and being engaged
Determine block, when described hybridizing box lid lid is set on described hybridizing box box body, described clamping block buckles into described fore shaft
Hybridizing box lid is fixedly installed on hybridizing box box body in groove, now described chip positioning block will be placed on described core
The biochip that piece is put in cavity is fixed.Above-mentioned latch components are simple in construction, using easy to operate.
The arc that the side of described chip placement cavity sets oriented described hybridizing box box body outer wall direction raised takes
Put groove.The arc picks and places groove and is easy to picking and placeing for biochip.
Described hybridization wet box includes being provided with cavity in box body, described box body, and a sidepiece of described box body is set
It is equipped with the box being movably provided with the opening being connected with described cavity, described opening for closing described cavity
Lid；
It is provided with biochip storing case in described cavity, the top of described biochip storing case is provided with multiple
The horizontal slot matched with described hybridizing box, described horizontal slot is described storing chamber, described horizontal slot
Insert port is arranged at described opening；
The bottom of described biochip storing case is provided with the long slot of level, and the long slot insert port of described level is set
At described opening, humidification disk can be provided with the long slot of described level pull；
Heating plate and heater circuit are provided with described box body, described heating plate is electrically connected with described heater circuit
Connect, the outside of described box body is provided with USB charging plugs, and described USB charging plugs are electrically connected with described heater circuit.
The box body and lid of described hybridization wet box use heat-barrier material, can play preferable insulation effect.
Above-mentioned hybridization wet box is simple in construction, sets and is open in box body sidepiece, is easy to picking and placeing for hybridizing box and humidification disk, lid
For closed cavity, the hybridization to biochip provides a constant environment；Biochip is set to put in the cavity of box body
Box, the top of the biochip storing case sets multiple horizontal slots matched with hybridizing box, and hybridizing box can be set pull
It is simple in construction in horizontal slot, it is easy to pick and place, while the horizontal slot has given hybridizing box one stable accommodation space, can
Effectively prevent hybridizing box from being shaken in crossover process, so that the success rate of hybridization has been effectively ensured；Biochip storing case
Bottom be provided with the long slot of level, the long slot of the level can be provided with humidification disk pull, can be given by the humidification disk
The hybridization of biochip provides the hybridization environment of a constant humidity；Heating plate and heater circuit are provided with box body, passes through the heating
Plate and related heater circuit provide the hybridization environment of a constant temperature to the hybridization of biochip, and box portion outside sets USB to charge
Plug, is charged by the USB charging plugs to heater circuit, simple in construction, easy to operate.
The upper surface of described horizontal slot and the insert port of the described long slot of level is provided with the arc raised up
The lower surface of the insert port of lug boss, described horizontal slot and the described long slot of level is provided with recessed to the arc of lower recess
The portion of falling into, described arc convex portion and the position in described arc-shaped recess portion is corresponding up and down.Arc convex portion and arc-shaped recess portion
The structure of phase structure is easy to fetching for hybridizing box and humidification disk.
The bottom of described lid is arranged on the bottom of described opening by gemel connection, in the top of described lid
End face is provided with hasp, and described upper opening portion is provided with the hasp locating slot being engaged with described hasp, when lid lid holds together,
Described hasp is arranged in described hasp locating slot, and described lid and the joint of described opening is provided with sealing
Circle.The connection of above-mentioned lid and manner are simple in construction, easy to operate, and sealing ring is set in the joint of lid and opening,
When ensureing that lid is closed, tray interior is sealing state, it is ensured that the sealing of hybridization environment.
It is provided with ventilative gap between the top and bottom of described biochip storing case, the center of described humidification box
Region is to lower recess.The moisture that humidification disk is produced can be preferably supplied to by hybridizing box by the ventilative gap, with preferably
Meet hybridization conditions；The middle section of humidification disk is to lower recess, and the depression can be used for containing water or the blotting paper of moistening.
Described hybridization wet box includes box body and lid is located at lid on described box body, described box body include chassis and
It is arranged in the accommodating chamber on described chassis, described accommodating chamber and is laterally set side by side with size identical front support seat with after
Support base, described front support seat and described rear support seat by described accommodating chamber be divided into from front to back front chamber, in
Between chamber and rear chamber, be provided with humidification mechanism in described intermediate cavity, the upper of described front support seat vertical is arranged at intervals
There are multiple preceding spacing blocks, described preceding spacing block is uniformly arranged in the upper surface of described front support seat, described rear support seat
It is upper be vertically arranged at intervals with multiple rear spacing blocks, described rear spacing block is uniformly arranged in the upper end of described rear support seat
Enclose and be set as between the rear spacing block described in adjacent two on face, two neighboring described preceding spacing block and vertical correspondence position
Heating plate and heater circuit, described heating plate and described heater circuit are provided with described storing chamber, described chassis
Electrical connection, the outside of described box body is provided with USB charging plugs, described USB charging plugs and described heater circuit electricity
Connection.Above-mentioned hybridization wet box it is simple in construction, pass through adjacent two on two neighboring preceding spacing block and vertical correspondence position
Enclosed afterwards between spacing block and be set as putting chamber, hybridizing box is placed on this, a stable accommodation space is provided to hybridizing box；In
Between be provided with humidification mechanism in chamber, the hybridization environment of a constant humidity is provided by the humidification mechanism to the hybridization of biochip；
Heating plate and heater circuit are provided with chassis, is provided by the heating plate and related heater circuit to the hybridization of biochip
The hybridization environment of one constant temperature, box portion outside sets USB charging plugs, is charged by the USB charging plugs to heater circuit, knot
Structure is simple, easy to operate.
Described humidification mechanism is water-absorbing sponge.Enough water is absorbed by water-absorbing sponge, and places it in intermediate cavity
In, the humidity that relative constancy is kept in hybridization wet box heating process is ensure that, to meet hybridization conditions.
Horizontal width between two neighboring described preceding spacing block, two neighboring described rear spacing block with it is described miscellaneous
The width of box is handed over to match, the vertical width of described intermediate cavity is less than the length of described hybridizing box.Said structure ensures
Hybridizing box, which can be stablized, to be seated in thereon.
The joint of described lid and described box body is provided with sealing ring.Set close in the joint of lid and box body
Seal, it is ensured that when lid is closed, tray interior is sealing state, it is ensured that the sealing of hybridization environment.
Compared with prior art, the advantage of the invention is that：Hybridizing box structure letter in above-mentioned biochip hybridizing device
It is single, by setting watch window on hybridizing box lid, hybridizing box lid is used directly as cover plate of the prior art, can
The generation of slide plate phenomenon is prevented effectively from, chip placement cavity is provided with hybridizing box box body, the chip placement cavity is applied to
While general biochip, material more thin biochip is applied equally to, when hybridizing box lid lid holds together, is passed through
The biochip being seated in chip placement cavity is fixed by chip positioning block, and providing one to biochip stable determines
Position, further avoids the generation at slide plate scene, it is ensured that the success rate of hybridization；There is provided by hybridizing wet box to the hybridization of biochip
One constant hybridization space, the auxiliary equipment extra without other saves cost；Set many on hybridizing box lid in addition
Individual watch window, realizes once progress multi-disc and hybridizes simultaneously.
Brief description of the drawings
Fig. 1 is structural representation of the hybridizing box under the state that covers in the present invention；
Fig. 2 is the structural representation of hybridizing box in the on-state in the present invention；
Fig. 3 is the structural representation of hybridization wet box in the embodiment of the present invention one；
Fig. 4 is the cross section structure diagram of hybridization wet box in the embodiment of the present invention one；
Fig. 5 is the structural representation of humidification box in the embodiment of the present invention one；
Fig. 6 is the structural representation of hybridization wet box in the embodiment of the present invention two；
Fig. 7 is the cross section structure diagram of hybridization wet box box body in the embodiment of the present invention two.
Embodiment
The present invention is described in further detail below in conjunction with accompanying drawing embodiment.
Embodiment one：As shown in Figure 1 and Figure 2, a kind of biochip hybridizing device, including hybridization wet box A and hybridizing box B, it is miscellaneous
Hand over to be horizontally disposed with wet box A and there are multiple storing chambers for being used to put hybridizing box B, hybridizing box B includes hybridizing box box body B1 and hybridization
It is provided with box lid B2, hybridizing box box body B1 outside the chip placement cavity B11 for putting biochip, hybridizing box lid B2
End face is arranged side by side multiple watch window B21 to lower recess, and watch window B21 edge is provided with up/down perforation
Well 12, hybridizing box lid B2 inner faces, which are provided with, to be used for the securing chip positioning block B22 in biochip position, chip
Locating piece B22 position is corresponding with chip placement cavity B11 position, hybridizing box box body B1 and hybridizing box lid B2 one end
Pass through hinge（Do not shown in figure）Connection, hybridizing box box body B1 is connected with the hybridizing box lid B2 other end by latch components.
In this particular embodiment, watch window B21 bottom face uses transparent material, when biochip is put into chip
When putting in cavity B11, watch window B21 is directed at the sample of biochip surface, watch window B21 bottom face and biological core
Gap is provided between the upper surface of piece, the gap forms chip conversion zone, when hybridizing box lid B2 lids are set to hybridizing box box
When on body B1, chip positioning block B22 lower surface and the upper surface of biochip are brought into close contact.Watch window B21 bottom faces are adopted
With transparent material, when biochip is put into chip placement cavity B11, operator passes through the transparent watch window B21
Sample area on biochip can be accurately placed in watch window B21 lower section, be easy to the solution containing hybridization composition to lead to
Cross well 12 and accurately enter chip conversion zone, contacted with the sample in biochip surface, complete hybridization, it is ensured that hybridization
Success rate；Chip positioning block B22 lower surface and the upper surface of biochip are brought into close contact simultaneously, it is ensured that biochip is fixed
The stability of position.
In this particular embodiment, the tank B12 for holding liquid is additionally provided with hybridizing box box body B1.Can be in the water
A certain amount of pure water is injected in groove B12, the hybridization to biochip provides the hybridization environment of a moistening.
In this particular embodiment, tank B12 is two, and two tank B12 are respectively symmetrically provided with recessed in chip placement
Chamber B11 both sides.Tank B12 is two, and two tank B12 are symmetrically arranged at chip placement cavity B11 both sides,
A certain amount of pure water is injected in tank B12 so that the humidity on chip placement cavity B11 both sides is able to unanimously.
In this particular embodiment, above-mentioned gap is 0.2mm.
In this particular embodiment, latch components include lock the seat B3, lock seat B3 being arranged on hybridizing box lid B2
Lower surface be provided with lock groove B31, be provided with hybridizing box box body B1 with latching groove B31 positions corresponding and be engaged
Clamping block B13, when hybridizing box lid B2 lids are set on hybridizing box box body B1, clamping block B13 is buckled into will be miscellaneous in fore shaft groove B31
Box lid B2 is handed over to be fixedly installed on hybridizing box box body B1, now chip positioning block B12 will be placed in chip placement cavity B11
Biochip be fixed.Above-mentioned latch components are simple in construction, using easy to operate.
In this particular embodiment, well 12 from top to bottom aperture gradually it is small be in funnel-form, be adapted with suction pipette head,
It is easy to the insertion of suction pipette head, while enabling solution accurately to drip to the sample position of biochip surface.
In this particular embodiment, chip placement cavity B11 side sets oriented hybridizing box box body B1 outer walls direction convex
The arc risen picks and places groove B14.The arc picks and places groove B14 and is easy to picking and placeing for biochip.
When specifically used, a certain amount of pure water is added in tank B12, biochip of the surface with sample is put into
In chip placement cavity, hybridizing box lid B2 is then covered, now, buckles into and fixes hybridizing box lid B2 in fore shaft groove B31
It is arranged on hybridizing box box body B1, now chip positioning block B12 compresses the biochip being placed in chip placement cavity B11
Fixed, whether the sample from transparent groove B21 on biochip is located at groove B21 lower section, if not having, opens
Hybridizing box lid B2, adjusts the position of biochip, until when hybridizing box lid B2 lids hold together, the sample on biochip is placed in recessed
Below groove B21, then the solution with hybridization composition is added dropwise in chip conversion zone by well 12.
In this particular embodiment, as shown in Figures 3 to 5, hybridization wet box A includes being provided with cavity in box body 4, box body 4,
One sidepiece of box body 4 is provided with the opening 41 being connected with cavity, opening 41 and is movably provided with for closed cavity
Lid 42；
In this particular embodiment, biochip storing case 5 is provided with cavity, the top of biochip storing case 5 is set
Multiple horizontal slots 51 matched with hybridizing box B are equipped with, horizontal slot 51 is the storing chamber for putting hybridizing box B, level
The insert port of slot 51 is arranged at opening 41；
In this particular embodiment, the bottom of biochip storing case 5 is provided with the long slot 52 of level, the long slot 52 of level
Insert port be arranged on opening 41 at, can be provided with humidification disk 6 in the long slot 52 of level pull；
In this particular embodiment, heating plate 7 and heater circuit are provided with box body 4（Do not shown in figure）, heating plate 7 with
Heater circuit is electrically connected, and the outside of box body 4 is provided with USB charging plugs 8, and USB charging plugs 8 are electrically connected with heater circuit.
In this particular embodiment, hybridization wet box A box body 4 and lid 42 uses heat-barrier material, can play preferably
Insulation effect.
In this particular embodiment, Temperature Controlling Chip is provided with heater circuit, passes through Temperature Controlling Chip control hybridization wet box A
Interior temperature, to provide an accurate isoperibol.
In this particular embodiment, the levels of box body 4 are provided with heating plate 7, improve the efficiency of heating surface so that levels
Temperature be consistent.
Above-mentioned hybridization wet box A is simple in construction, and opening 41 is set in the sidepiece of box body 4, is easy to taking for hybridizing box B and humidification disk 6
Put, lid 42 is used for closed cavity, the hybridization to biochip provides a constant environment；Life is set in the cavity of box body 4
Thing chip placement box 5, the top of the biochip storing case 5 sets multiple horizontal slots 51 matched with hybridizing box B, hybridization
Box 51 can be arranged in horizontal slot 51 pull, simple in construction, be easy to pick and place, while the horizontal slot 51 has given hybridizing box B
One stable accommodation space, can effectively prevent hybridizing box B from being shaken in crossover process, so that hybridization has been effectively ensured
Success rate；The bottom of biochip storing case 5 is provided with the long slot 52 of level, the long slot 52 of the level and can be provided with pull
Humidification disk 6, the hybridization environment of a constant humidity can be provided by the humidification disk 6 to the hybridization of biochip；It is provided with box body 4
Heating plate 7 and heater circuit, constant temperature is provided by the heating plate 7 and related heater circuit to the hybridization of biochip
Hybridize environment, box portion outside sets USB charging plugs 8, is charged by the USB charging plugs 8 to heater circuit, simple in construction,
It is easy to operate.
In this particular embodiment, the upper surface of horizontal slot 51 and the insert port of the long slot 52 of level is provided with convex
The lower surface of the insert port of the arc convex portion c risen, horizontal slot 51 and the long slot 52 of level is provided with the arc to lower recess
Depressed part d, arc convex portion c and arc-shaped recess portion d position are corresponding up and down.Arc convex portion c and arc-shaped recess portion d phase structures
Structure be easy to fetching for hybridizing box B and humidification disk 6.
In this particular embodiment, the bottom of lid 42 passes through hinge（Do not shown in figure）Connection is arranged under opening 41
Portion, the upper interior end face of lid 42 is provided with hasp 421, and 41 tops of opening are provided with the hasp positioning being engaged with hasp 421
Groove 411, when the lid of lid 42 holds together, hasp 421 is buckled into hasp locating slot 411, and the joint of lid 42 and opening 41 is provided with close
Seal e.The connection of above-mentioned lid 42 and manner are simple in construction, easy to operate, are set in lid 42 and the joint of opening 41
Put sealing ring e, it is ensured that when lid 42 is closed, the inside of box body 4 is sealing state, it is ensured that the sealing of hybridization environment.
In this particular embodiment, ventilative gap, humidification box are provided between the top and bottom of biochip storing case 5
6 middle section 61 is to lower recess.The moisture that can be produced humidification disk 6 by the ventilative gap is preferably supplied to hybridization
Box, preferably to meet hybridization conditions；The middle section 61 of humidification disk 6 is to lower recess, and the depression can be used for containing water or wet
The blotting paper of profit.
In this particular embodiment, the top of biochip storing case 5 is divided into multiple rows of structure from top to bottom, per row of horizontal
Multiple horizontal slots 51 are set side by side with, the long slot 52 of level is arranged under bottom horizontal slot 51, each horizontal slot
Ventilative gap is provided between 51, and the moisture that humidification disk can be produced by the ventilative gap is preferably supplied to hybridization
Box, preferably to meet hybridization conditions.
Embodiment two：Other parts are identical with embodiment one, and its difference is, as shown in Figure 6, Figure 7, hybridize wet box
A includes box body A4 and covers the lid A5 being located on box body A4, and box body A4 includes chassis A41 and the receiving being arranged on the A41 of chassis
Size identical front support seat A6 and rear support seat A7, front support seat A6 and rear branch are laterally set side by side with chamber A42, accommodating chamber
Support seat A7 to be arranged at intervals, between front support seat A6 rear end face, rear support seat A7 front end face and accommodating chamber A42 both sides inwall
Enclose and be set as intermediate cavity A421, humidification mechanism is provided with intermediate cavity A421, front support seat the upper of A6 is vertically arranged at intervals with
Multiple preceding spacing block A61, preceding spacing block A61 be uniformly arranged front support seat A6 upper surface, rear support seat A7 it is upper it is vertical between
Every being provided with multiple rear spacing block A71, rear spacing block A71 is uniformly arranged in rear support seat A7 upper surface, two neighboring preceding point
After adjacent two on spacer block A61 and vertical correspondence position the storing being set as putting hybridizing box B is enclosed between spacing block A71
Heating plate A8 and heater circuit are provided with chamber, chassis A41（Do not shown in figure）, heating plate A8 electrically connects with heater circuit, box
Body A4 outside is provided with USB charging plugs A9, USB charging plug A9 and electrically connected with heater circuit.Above-mentioned hybridization wet box A knot
Structure is simple, is set by being enclosed between spacing block A71 after adjacent two on two neighboring preceding spacing block A61 and vertical correspondence position
Into chamber is put, hybridizing box B is placed on this, a stable accommodation space is provided to hybridizing box B；Set in intermediate cavity A421
Humidification mechanism is equipped with, the hybridization environment of a constant humidity is provided by the humidification mechanism to the hybridization of biochip；Set in the A41 of chassis
Heating plate A8 and heater circuit are equipped with, one is provided to the hybridization of biochip by heating plate A8 and related heater circuit
USB charging plug A9 are set outside the hybridization environment of constant temperature, box body A4, charged by the USB charging plugs A9 to heater circuit,
It is simple in construction, it is easy to operate.
In this particular embodiment, humidification mechanism is water-absorbing sponge（Do not shown in figure）.Absorb enough by water-absorbing sponge
Water, and place it in intermediate cavity A421, it ensure that and relative constancy is kept in heating process in hybridization wet box A
Humidity, to meet hybridization conditions.
In this particular embodiment, the level between two neighboring preceding spacing block A61, two neighboring rear spacing block A71 is wide
The width spent with hybridizing box B matches, and intermediate cavity A421 vertical width is less than hybridizing box B length.Said structure ensures
Hybridizing box B, which can stablize, to be seated in thereon.
In this particular embodiment, lid A5 and box body A4 joint is provided with sealing ring（Do not shown in figure）.In box
The joint for covering A5 and box body A4 sets sealing ring, it is ensured that when lid A5 is closed, and is sealing state inside box body A4, it is ensured that hybridization
The sealing of environment.
In this particular embodiment, Temperature Controlling Chip is provided with heater circuit, passes through Temperature Controlling Chip control hybridization wet box A
Interior temperature, to provide an accurate isoperibol.
Embodiment three：Other parts are identical with embodiment one, embodiment two, and its difference is that gap is 0.3mm.
Example IV：Other parts are identical with embodiment one, embodiment two, and its difference is that gap is 0.4mm.
Embodiment five：Other parts are identical with embodiment one, embodiment two, and its difference is that gap is 0.5mm.
Claims (8)
1. a kind of biochip hybridizing device, it is characterised in that including hybridizing water in wet box and hybridizing box, described hybridization wet box
Flat to be provided with multiple storing chambers for being used to put described hybridizing box, described hybridizing box includes hybridizing box box body and hybridizing box box
It is provided with lid, described hybridizing box box body outside the chip placement cavity for putting biochip, described hybridizing box lid
End face is arranged side by side multiple watch windows to lower recess, and the edge of described watch window is provided with up/down perforation
Well, described hybridizing box lid inner face is provided with for by the described securing chip positioning in biochip position
Block, the position of described chip positioning block is corresponding with the position of described chip placement cavity, described hybridizing box box body with
One end of described hybridizing box lid is by gemel connection, the other end of described hybridizing box box body and described hybridizing box lid
Connected by latch components；The bottom face of described watch window uses transparent material, when described biochip is put into institute
When in the chip placement cavity stated, the sample area of the described biochip surface of described watch window alignment, described observation
Gap is provided between the upper surface of the bottom face of window and described biochip, described gap forms chip reaction zone
Domain, when described hybridizing box lid lid is set on described hybridizing box box body, the lower surface of described chip positioning block and institute
The upper surface for the biochip stated is brought into close contact；The tank for holding liquid is additionally provided with described hybridizing box box body.
2. a kind of biochip hybridizing device as claimed in claim 1, it is characterised in that described hybridization wet box includes box body,
Cavity is provided with described box body, a sidepiece of described box body is provided with the opening being connected with described cavity, institute
The lid for closing described cavity is movably provided with the opening stated；
Biochip storing case is provided with described cavity, the top of described biochip storing case is provided with multiple and institute
The horizontal slot that the hybridizing box stated matches, described horizontal slot is described storing chamber, the insertion of described horizontal slot
Mouth is arranged at described opening；
The bottom of described biochip storing case is provided with the long slot of level, and the long slot insert port of described level is arranged on institute
At the opening stated, humidification disk can be provided with the long slot of described level pull；
Heating plate and heater circuit are provided with described box body, described heating plate is electrically connected with described heater circuit, institute
The outside for the box body stated is provided with USB charging plugs, and described USB charging plugs are electrically connected with described heater circuit.
3. a kind of biochip hybridizing device as claimed in claim 2, it is characterised in that described horizontal slot and described
The upper surface of the insert port of the long slot of level is provided with the arc convex portion raised up, described horizontal slot and described water
The lower surface of the insert port of flat long slot is provided with the arc-shaped recess portion to lower recess, described arc convex portion and described arc
The position of shape depressed part is corresponded to up and down.
4. a kind of biochip hybridizing device as claimed in claim 2, it is characterised in that the bottom of described lid is by closing
Page connection is arranged on the bottom of described opening, and the upper interior end face of described lid is provided with hasp, described upper opening portion
The hasp locating slot being engaged with described hasp is provided with, when lid lid holds together, described hasp, which is arranged on described hasp, to be determined
In the groove of position, described lid and the joint of described opening is provided with sealing ring.
5. a kind of biochip hybridizing device as claimed in claim 2, it is characterised in that described biochip storing case
It is provided with ventilative gap between top and bottom, the middle section of described humidification disk is to lower recess.
6. a kind of biochip hybridizing device as claimed in claim 1, it is characterised in that described hybridization wet box includes box body
The lid on described box body, the accommodating chamber that described box body includes chassis and is arranged on described chassis, institute are located at lid
Laterally it is set side by side with size identical front support seat and rear support seat in the accommodating chamber stated, described front support seat and described
Described accommodating chamber is divided into front chamber, intermediate cavity and rear chamber, described intermediate cavity by rear support seat from front to back
Humidification mechanism is inside provided with, described the upper of front support seat is vertically arranged at intervals with multiple preceding spacing blocks, described preceding spacing block
It is uniformly arranged in the upper surface of described front support seat, described the upper of rear support seat is vertically arranged at intervals with multiple rear separations
Block, described rear spacing block is uniformly arranged in the upper surface of described rear support seat, and two neighboring described preceding spacing block is with erecting
Enclosed between the rear spacing block described in adjacent two on straight correspondence position and be set as setting in described storing chamber, described chassis
There are heating plate and heater circuit, described heating plate is electrically connected with described heater circuit, and the outside of described box body is provided with
USB charging plugs, described USB charging plugs are electrically connected with described heater circuit.
7. a kind of biochip hybridizing device as claimed in claim 6, it is characterised in that two neighboring described preceding spacing block,
The width of horizontal width and described hybridizing box between two neighboring described rear spacing block matches, described intermediate cavity
Vertical width be less than described hybridizing box length.
8. a kind of biochip hybridizing device as claimed in claim 6, it is characterised in that described lid and described box body
Joint be provided with sealing ring.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610273330.7A CN105772127B (en) | 2016-04-28 | 2016-04-28 | A kind of biochip hybridizing device |
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| CN201610273330.7A CN105772127B (en) | 2016-04-28 | 2016-04-28 | A kind of biochip hybridizing device |
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| CN105772127A CN105772127A (en) | 2016-07-20 |
| CN105772127B true CN105772127B (en) | 2017-10-31 |
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| CN110927373B (en) * | 2019-11-26 | 2021-10-15 | 北京化工大学 | Full-automatic multi-index joint detection micro-fluidic chip and device |
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| CN105772127A (en) | 2016-07-20 |
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Effective date of registration: 20180724 Address after: 315201 No. 777, Zhong Guan Xi Road, Zhuang City Street, Zhenhai District, Ningbo, Zhejiang. Patentee after: Ningbo Anxin Biological Technology Co., Ltd. Address before: 315211 Fenghua Road, Jiangbei District, Ningbo, Zhejiang Province, No. 818 Patentee before: Ningbo University |
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Effective date of registration: 20190514 Address after: 315040 Ningbo New Materials Innovation Center East District, Ningbo High-tech Zone, Zhejiang Province, No. 1 11-2-1 Patentee after: Zhejiang Zhenghegu Biotechnology Co., Ltd. Address before: 315201 No. 777, Zhong Guan Xi Road, Zhuang City Street, Zhenhai District, Ningbo, Zhejiang. Patentee before: Ningbo Anxin Biological Technology Co., Ltd. |