CN105764342A - Active compound combinations - Google Patents

Abstract

The present invention primarily relates to active compound combinations comprising (A) propineb and a constituent (B) comprising (B1) one or more salts containing boron (B), and (B2) one or more salts containing manganese (Mn). More specifically, the active compound combinations according to the invention are useful for improving plant quality. The present invention further relates to corresponding methods and uses of the active compound combinations according to the present invention.

Description

Active agent combinations

The invention mainly relates to active agent combinations, it includes (A) Propineb and composition (B), and described composition (B) includes (B1) one or more salt containing boron (B) and (B2) one or more salt containing manganese (Mn).More specifically, the active agent combinations of the present invention can be used for improving plant quality.The invention still further relates to the correlation method of the active agent combinations of the present invention and purposes.

The molecular formula of Propineb is (C₅H₈N₂S₄Zn)_x, it is the propylidene double; two (zinc dithiocarbamate) (CAS registration number 12071-83-9) of polymerization and can be described by following chemical formula (I):

Known Propineb has fungicide performance and can be used for preventing and treating various plant disease (referring to such as GB935981).

WO2011/107443A1 teaches the Propineb purposes for the preventative process of the physiology in zinc deficiency situation and therapeutic treatment.Wherein also reporting, except physiologic therapy effect, Propineb processes and brings higher yield.For the experimental purpose in greenhouse, in WO2011/107443A1, having sowed certain plants and made it grow in rock wool, described rock wool Hoagland solution applies fertilizer with drip irrigation.Then, after certain time intervals, Propineb is used by sprinkling it is applied to each plant.

WO2012/089724A1 relates to improve the method for plant quality, the method includes adopting the active component containing micronutrient of plant quality improvement amount to process crop plants, and/or process the growth place of crop plants or the place of expectation growth, and/or process propagulum.

Illustrate on the 3rd international zinc symposium of India Hyderabad in October, 2011 title for "-AFungicideImprovingZincNutritioninPlants " poster (http://www.zinccrops2011.org/presentations/2011_zinccrops2011_g oertz_abstract.pdf) in.Wherein, can conclude that, namely except its broad spectrum fungicidal activity, use under business rate of application(effective ingredient: Propineb) has notable active influence for the growth of crop of plantation, growth and quality in zinc deficiency situation.UseThe zinc deficiency in plant can be avoided.Result proves, when disease, when peasant's applications of fungicides,Also can provide, for plant, the zinc nutrition improved, reduce the demand for zinc fertilizer.

DE2558385A1 relates to the compositions providing the crop yield improved, and said composition contains (1) some dithiocar-bamate (dithiocarbamidates) and (2) mineral acid or organic acid manganese (Mn) salt.

Additionally, WO2005/070204 reports micronutrient in insecticides for reducing phytotoxic purposes.

Force at the environment on modern crop production compositions and economic requirement improves constantly.This is not only related to, for instance, action spectrum, toxicity, selectivity, rate of application etc., also relate to the plant quality improved, the yield particularly increased, and/or relate to the plant vigor of improvement.

It has now surprisingly been found that the active agent combinations of the present invention, not only bring the enhancing in the action spectrum of phytopathogen to be prevented and treated, and achieve the plant quality of improvement, the yield particularly improved, and/or the plant vigor improved.The active agent combinations of the present invention has cooperative characteristics, such as the plant performance improved, for instance better growth, the yield of gathering increased, more flourishing root system, bigger leaf area, greener leaf, stronger branch, particularly greener leaf.

Therefore, the present invention relates to active agent combinations, comprising:

(A) Propineb,

With composition (B), it includes

(B1) one or more contain the salt of boron (B), and

(B2) one or more contain the salt of manganese (Mn),

In the active agent combinations of the present invention, the one of composition (B1), several or all components are preferably selected from the oxide of boron and boric acid class and their salt.

In the active agent combinations of the present invention, the one of composition (B1), several or all components are preferably selected from diboron trioxide (B₂O₃)、H₃BO₃、H₂B₄O₇, (preferred Borax, also referred to as sodium borate, sodium tetraborate or disodium tetraborate, it is preferable that borax anhydrous (Na for boric acid sodium salt₂B₄O₇), borax pentahydrate (Na₂B₄O₇·5H₂Or borax decahydrate (Na O)₂B₄O₇·10H₂O)), boric acid potassium salt (preferred K₂B₄O₇And hydrate, it is preferable that its tetrahydrate K₂B₄O₇·4H₂O), boric acid calcium salt (preferred CaB₄O₇、Ca₃(BO₃)₂And hydrate), boric acid magnesium salt (preferred MgB₄O₇、Mg₃(BO₃)₂And hydrate), potassium borohydride, sodium borohydride, potassium borotartrate and nickel borate (II).

In the preferred active agent combinations of the present invention, the one of composition (B2), several or all components are selected from manganese (II) salt, it is preferable that selected from manganese acetate (II), manganese sulfate (II), manganese chloride (II), manganese nitrate (II) and manganese phosphate (II).

In the preferred active agent combinations of the present invention,

Composition (B1) includes disodium tetraborate and/or disodium tetraborate hydrate or is made up of disodium tetraborate and/or disodium tetraborate hydrate,

And/or

Composition (B2) includes manganese sulfate (II) and/or manganese chloride (II) or is made up of manganese sulfate (II) and/or manganese chloride (II).

If the active component in the active agent combinations of the present invention exists with certain weight ratio, described cooperative effect is especially notable.

Therefore, in the preferred active agent combinations of the present invention, composition (A) relative to the weight ratio of the boron of composition (B) and the gross weight of manganese in the scope of 1250:1 to 25:1, preferably in the scope of 1000:1 to 50:1, more preferably in the scope of 500:1 to 75:1, and particularly preferably in, in the scope of 350:1 to 100:1, being each based on the gross weight gauge of described active agent combinations.

In the preferred active agent combinations of the present invention, wherein the total amount of boron [composition (B1)] relative to the weight ratio of the total amount of magnesium [composition (B2)] in the scope of 3:1 to 1:3, preferably in the scope of 2:1 to 1:2, more preferably in the scope of 3:2 to 2:3, even more preferably in the scope of 4:3 to 3:4, and most preferably at, in the scope of 5:4 to 4:5, being each based on the gross weight gauge of described active agent combinations.

In preferred embodiments, the active agent combinations of the present invention also includes:

-the one in zinc (Zn), copper (Cu), ferrum (Fe), molybdenum (Mo), selenium (Se), aluminum (Al), cobalt (Co) and nickel (Ni), several or all of micronutrient

And/or

-the one in nitrogen (N), phosphorus (P) and potassium (K), several or all of macronutrient.

Preferably, the active agent combinations of the present invention also includes:

-nitrogen (N), phosphorus (P) and potassium (K),

With

-at least one in zinc (Zn), copper (Cu), ferrum (Fe), molybdenum (Mo), selenium (Se), aluminum (Al), cobalt (Co) and nickel (Ni), preferably two or more, the micronutrient of more preferably three kinds or more kind.

Preferably, the active agent combinations of the present invention includes one or more of selected from following component: zinc oxide, zinc acetate, Zinc dibenzoate., zinc chloride, zinc citrate, zinc nitrate, zinc salicylate；Copper acetate, copper butyrate, copper chlorate, copper chloride, copper citrate, copper gluconate, cupric glycinate, copper nitrate, copper salicylate, cuprous acetate, Cu-lyt.；Iron chloride, ferric citrate, ferric fructose, ferric glycerylphosphate, ferric nitrate, iron sesquioxide, saccharated iron oxide, ferrous chloride, ferrous citrate, ferrous fumarate, ferrous gluconate, ferrous succinate；Molybdic acid, calcium molybdate, potassium molybdate, sodium molybdate；Sodium selenite, potassium selenite, sodium selenate, potassium selenate；Aluminum phosphate, aluminium silicate；Acetic acid height cobalt, cobalt acetate, cobaltous chloride, cobalt oxalate, Potassium cobaltosulfate, cobaltous sulfate；Nickel dichloride. (II) and nickel sulfate (II).

According to the present invention, statement " conjugate " represents the various combinations of composition (A) and (B), such as single " namely with mixture " form, the merging being made up of the independent preparation of single-activity compound spraying mixture form (such as " bucket mixes thing "), and when single-activity composition is sequentially used (at short notice, it is preferable that use one by one in less than two hours) merge use mode.

The present invention relates to by the active agent combinations of the present invention being applied to the new method improving plant quality in plant, plant parts and/or its habitat.

Term " plant quality " (quality of plant) is defined as the situation of described crop plants and/or its product, it is individually determined by several aspects or is determined by the mode combined mutually, such as yield (Biomass such as increased, the content of the content of the valuable components of increase and/or the improvement of some composition or composition) and plant vigor (plant growing such as improved and/or greener leaf).

For weighing the quality of plant, particularly an index of plant situation is its yield." yield " is interpreted as any plant parts with economic worth by plant production or product, for instance fruit in seed, leaf, root, suitable meaning, vegetable, nut, seed, timber (such as when forestry) or even flowers (such as when gardening and ornamental plant).Described plant product can be further employed after gathering again and/or process.

According to the present invention, " yield of increase " of crop plants refers to does not use the yield of the same products of the plant of the described active component containing micronutrient relative to producing under the same conditions, and the yield of the product of each crop plants increases measurable amount.The yield increased especially can be characterized by the performance of the crop plants of following improvement:

The plant weight increased,

The plant height increased,

The Zn content increased,

The iron content increased,

The calcium content increased,

The Biomass increased, for instance higher fresh weight (FW) and/or dry weight (DW),

Higher grain yield,

Higher acidity,

Higher anthocyanidin content,

More tiller,

Bigger blade,

The shoot growth increased,

(such as the soluble protein) increased,

The oil content increased,

The content of starch increased,

The pigment content increased,

The nutrient content increased,

The protein content increased,

Vitamin content (the such as vitamin B increased₁、B₂, C and E),

The content of fatty acid increased,

The metabolite content increased,

The carotenoid content (such as vitamin A) increased,

The essential amino acids amount increased,

The nutrient composition improved,

The protein composition improved,

The fatty acid composition improved,

The metabolite composition improved,

The carotenoid composition improved,

The sugar composition improved,

The aminoacid composition improved,

Fruit color that is that improve or that optimize,

The leaf color improved,

Higher storage capability,

Gather the higher processability of product.

According to one embodiment of the invention, depend on the type of the performance improved, yield increase at least 5% or more, preferably 10% or more, more preferably 15% or more, even more preferably from 20% or more, and even more preferably from 25% or more, each compared to corresponding untreated check plant.

For weighing the quality of plant, particularly another index of crop plants situation is " plant vigor ".Described plant vigor is embodied in several aspect, for instance total visual appearance and growth.The plant vigor improved especially can be characterized by the plant performance of following improvement:

The plant life power improved,

The plant growing improved,

The development of plants improved,

The visual appearance improved,

The plant thribble (less plant is toppled over/lodges) improved,

That improves emerges,

The root growth strengthened and/or more flourishing root system,

The dross strengthened, especially root nodule bacteria dross,

Bigger blade,

Bigger size,

The plant weight increased,

The fresh weight (FW) increased

The dry weight (DW) increased,

The plant height increased,

The tiller number increased,

The shoot growth increased,

The root growth (root system of extension) increased, the yield increased when growth in poor soil or under unfavourable weather,

The photosynthetic activity strengthened,

The pigment content (such as chlorophyll content) strengthened,

Blooming earlier,

Result earlier,

The rudiment with improvement earlier,

Grain Ripening earlier,

Better size distribution,

Higher grain hardness,

The self-defense mechanism improved,

The stress tolerance improved and plant to biological and abiotic stress factor (as fungus, antibacterial, virus, insecticide, heat stress, cold coerce, drought stress, ultraviolet are coerced and/or salt stress) resistance,

Less unproductive tiller,

Less dead basal leaf,

Less input needs (such as chemical fertilizer or water),

Greener leaf,

Full maturity under the trophophase shortened,

Less fertilizer needs,

Less seed demand,

Easier gather,

Accelerating the ripening faster and evenly,

The longer shelf-life,

Longer panicle,

Retarding ageing,

Higher and/or the more productivity tiller,

Better composition extractibility,

The seed quality (producing for seed for sowing in season subsequently) improved,

The suppression that the ethylene yield reduced and/or ethylene are received by plant,

Higher brightness,

More intensive color,

The quality improved,

Higher hardness,

Higher brix value.

According to one embodiment of the invention, depend on the type of the performance improved, described plant vigor increase at least 5% or more, preferably 10% or more, more preferably 15% or more, even more preferably from 20% or more, and even more preferably from 25% or more, each compared to corresponding untreated check plant.

Depend on handled plant, more preferably increase the Q factor different from other plant.It follows that some Q factors will be mentioned according to handled crop plants.

Preferably, the active agent combinations adopting the present invention processes following plant and plant parts: corn such as Semen Tritici aestivi, Fructus Hordei Vulgaris, rye (Secale cereale L.), Triticale hexaploide Lart., Sorghum vulgare Pers./foxtail millet and Herba bromi japonici, Semen Maydis, Cotton Gossypii, Semen sojae atricolor, rice, Rhizoma Solani tuber osi, Helianthi, Kidney bean, coffee, Radix Betae class (such as sugar beet and fodder beet), Semen arachidis hypogaeae, Brassica campestris L, fruit (such as Fructus Mali pumilae, pears and citrus fruit), vegetable (such as Fructus Lycopersici esculenti, Fructus Cucumidis sativi, Bulbus Allii Cepae and Caulis et Folium Lactucae sativae), meadow and ornamental plant (referring also to hereafter).

Process for corn (seed), it is preferable that Semen Tritici aestivi, Fructus Hordei Vulgaris, rye (Secale cereale L.), Triticale hexaploide Lart. and Herba bromi japonici, it is particularly preferred to Semen Maydis and rice.Furthermore it is preferred that process the plant and the plant parts that belong to Solanaceae kind (Solanaceaesp.) (such as Fructus Lycopersici esculenti, Rhizoma Solani tuber osi, Fructus Piperis, Fructus Capsici, Fructus Solani melongenae, Nicotiana tabacum L.) according to the present invention.

According to one embodiment of the invention, for Rhizoma Solani tuber osi, it is preferred that Q factor is:

The protein content (such as soluble protein) increased,

The content of starch increased,

The Biomass increased, such as higher fresh weight (FW) and/or dry weight (DW),

The Zn content increased,

Better size distribution.

According to one embodiment of the invention, for rice, it is preferred that Q factor is:

The carotenoid content (such as vitamin A) increased,

The Zn content increased,

The iron content increased,

The protein content (such as soluble protein) increased,

The grain hardness (less disruptive force) improved.

According to one embodiment of the invention, for Semen Tritici aestivi, it is preferred that Q factor is:

The carotenoid content (such as vitamin A) increased,

The Zn content increased,

The iron content increased,

The protein content (such as soluble protein) increased,

The grain hardness (less disruptive force) improved.

According to one embodiment of the invention, for Semen Maydis/Semen Maydis (maize), it is preferred that Q factor is:

The carotenoid content (such as vitamin A) increased,

The Zn content increased,

The iron content increased,

The protein content (such as soluble protein) increased,

The oil content increased,

The content of starch increased.

According to one embodiment of the invention, for Fructus Mali pumilae, it is preferred that Q factor is:

The Zn content increased,

The calcium content increased,

More intensive color and luster,

The quality improved,

Higher hardness,

Higher brix value.

According to one embodiment of the invention, for Citrus Plants, it is preferred that Q factor is:

The Zn content increased,

The Vitamin C content increased,

More intensive color and luster,

The quality improved,

Higher hardness,

Higher brix value.

According to one embodiment of the invention, for Fructus Lycopersici esculenti, Fructus Cucumidis sativi and Fructus Piperis, it is preferred that Q factor is:

Better size uniformity,

More intensive color and luster,

The quality improved,

Higher hardness,

Higher brix value,

The root growth strengthened,

The Zn content increased,

The calcium content increased.

According to one embodiment of the invention, for Fructus Vitis viniferae/liana, it is preferred that Q factor is:

Higher anthocyanidin content,

Higher acidity,

Higher Zn content,

Higher brix value,

Higher hardness,

More intensive color and luster,

The quality improved,

Better mouthfeel.

The invention still further relates to compositions, said composition includes the active agent combinations of the present invention of effective dose and preferred non-phytotoxic amount.These compositionss are also applied for preventing and treating unwanted microorganism, especially unwanted fungus and antibacterial.Described preferred Fungicidal composition includes the auxiliary agent, solvent, carrier, surfactant and/or the filler that are agriculturally suitable for.

In the context of the present invention, " harmful microbe preventing and treating " refers to compared with untreated plant, minimizing in harmful microorganism infects, it is measured with fungicidal efficiency, infect and preferably reduce 25-50% (compared with untreated plant), more preferably reduce 40-79% (compared with untreated plant)；Even further preferably, harmful microbe infects almost is suppressed (80-100%) completely or up hill and dale.Harmful microbe preventing and treating can be curative, namely for processing the plant infected；Or protectiveness, namely for protecting the plant not yet infected.

" effectively but the amount of plant-less toxicity " refers to the fungal disease being enough to prevent and treat plant in a satisfactory manner or eradicates the amount of fungal disease completely, and this amount will not cause any significant phytotoxicity symptom simultaneously.Generally, this rate of application can change in relatively wide scope.This depends on several factor, for instance depend on the component of the active agent combinations of fungus to be prevented and treated, plant, weather conditions and the present invention.

On the other hand, the present invention relates to compositions, comprising:

The active agent combinations of (i) present invention, it is preferable that as defined in one of above-mentioned preferred embodiment,

(ii) water, and

(iii) one or more adjuvants (auxiliary agent), it is preferable that one or more are selected from the adjuvant of organic solvent, surfactant, inorganic carrier, organic carrier and other filleies.

Preferably, the compositions of the present invention includes following total amount:

The active agent combinations of (i) present invention, it is preferable that as defined in one of above-mentioned preferred embodiment, it is in the scope of 0.05-0.5 weight %, it is preferable that in the scope of 0.1-0.3 weight %,

And/or

(ii) water, it is in the scope of 70-99.9 weight %, it is preferable that in the scope of 85-99.8 weight %,

It is each based on the gross weight gauge of described compositions.

It is highly preferred that the compositions of the present invention includes following total amount:

The active agent combinations of (i) present invention, it is preferable that as defined in one of above-mentioned preferred embodiment, it is in the scope of 0.1-0.3 weight %,

And/or

(ii) water, it is in the scope of 70-99.9 weight %, it is preferable that in the scope of 85-99.8 weight %,

It is each based on the gross weight gauge of described compositions.

The compositions of the present invention can obtain preferably by following methods, the method is characterized in that and comprises the following steps:

I () provides (A) Propineb, (B1) one or more contain the compound of boron (B), and (B2) one or more contain the compound of manganese (Mn),

Or

There is provided the active agent combinations of the present invention, it is preferable that as defined in one of preferred embodiment,

(ii) water is provided,

(iii) one or more adjuvants (auxiliary agent) are provided, it is preferable that one or more are selected from the adjuvant of organic solvent, surfactant, inorganic carrier, organic carrier and other filleies,

And blending ingredients (i) is to (iii), thus obtain the compositions of the present invention.

Suitable organic solvent includes the institute's polarized and the non-polar organic solvent that are generally used for preparation purpose.Preferably, described solvent is selected from ketone, for instance methyl iso-butyl ketone (MIBK) and Ketohexamethylene；Amide, for instance dimethylformamide and alkanoic acid amide, for instance spectrasolv DMDA and N, N-dimethyloctanamide；Also has cyclic solvent, for instance N-Methyl pyrrolidone, NOP, N-dodecylpyrrolidone, N-Octylcaprolactam, N-dodecyl caprolactam and butyrolactone；Also has intensive polar solvent, for instance dimethyl sulfoxide；And aromatic hydrocarbon, for instance dimethylbenzene, SolvessoTM, mineral oil (such as white spirit, oil, alkylbenzene and bobbin oil)；And ester, for instance propylene glycol methyl ether acetate, dibutyl adipate, Exceed 600, heptyl acetate, tri-n-butyl citrate and n-butyl phthalate；And alcohols, for instance benzylalcohol and 1-methoxy-2-propanol.

According to the present invention, carrier is natural or synthesis, organic or inorganic material, in order to obtain the better suitability, is especially adapted for use in and is applied to plant or plant parts or seed, is mixed with carrier by active component or combine.Described carrier, can be solid or liquid, it is common that inertia and shall apply to agricultural.

Useful solid or liquid-carrier include: such as ammonium salt and natural rock stone powder, such as Kaolin, clay, Talcum, Chalk, quartz, attapulgite, montmorillonite or kieselguhr；With synroc powder, such as silicon dioxide in small, broken bits, aluminium oxide and naturally occurring or synthetic silicate；Resin；Wax；Solid fertilizer；Water；Alcohols (particularly butanol)；Organic solvent；Mineral oil and vegetable oil, and their derivant.It is equally useful the mixture of these carriers.

Suitable solid packing and carrier include mean diameter between 0.005 to 20 μm, preferably in the inorganic particle between 0.02 to 10 μm, such as carbonate, silicate, sulfate and oxide, such as ammonium sulfate, ammonium phosphate, carbamide, calcium carbonate, calcium sulfate, magnesium sulfate, magnesium oxide, aluminium oxide, silicon dioxide, so-called particulate silicon dioxide, silica dioxide gel, naturally occurring or synthetic silicate and aluminosilicate and plant product, as flour, wood powder/sawdust and cellulose powder.

Useful solid carrier for granule includes: such as pulverize the natural rock (such as calcite, marble, Pumex, meerschaum, dolomite) of also classification and inorganic and organic dust synthesis granule, also has the granule of organic substance (such as wood flour, Exocarpium cocois (Cocos nucifera L), corncob and tobacco stem).

Useful liquid gas filler or carrier are those is the liquid of gaseous state under standard temperature and normal pressure, for instance aerosol propellants is halogenated hydrocarbons such as, and butane, propane, nitrogen and carbon dioxide.

In the formulation; viscosifier can be used; such as carboxymethyl cellulose; and natural and synthesis with the polymer (such as arabic gum, polyvinyl alcohol and polyvinyl acetate) of powder, granule or latex form, or other natural phospholipids (such as cephalin and lecithin) and the phospholipid that synthesizes.Other additive can be mineral oil and vegetable oil.

Organic solvent can also be adopted as cosolvent.Useful liquid flux is essentially: aromatic hydrocarbons, such as dimethylbenzene, toluene or alkylnaphthalene；Chlorinated aromatic hydrocarbons and chlorinated aliphatic hydrocarbons, such as chlorobenzene, vinyl chloride or dichloromethane；Aliphatic hydrocarbons such as hexamethylene or paraffin, for instance mineral oil fractions, mineral oil and vegetable oil；Alcohols, for instance butanol or ethylene glycol and their ether and ester；Ketone is acetone, methyl ethyl ketone, methyl iso-butyl ketone (MIBK) or Ketohexamethylene such as；And/or intensive polar solvent, such as dimethylformamide and dimethyl sulfoxide.

The active agent combinations of the present invention also can comprise other components, for instance surfactant.Useful surfactant is emulsifying agent and/or formation of foam agent, dispersant or the wetting agent with ion or non-ionic nature, or the mixture of these surfactants.The example of these surfactants is polyacrylic salt, the salt of lignosulphonic acid, the salt of phenolsulfonic acid or the salt of LOMAR PWA EINECS 246-676-2, the condensation polymer of oxirane and fatty alcohol or fatty acid or fatty amine, the phenol (preferred alkylphenol or aryl phenol) replaced, the salt of sulfosuccinate, taurine derivatives (preferred alkyltaurate), the phosphate ester of polyethoxylated alcohols or phenols, the fatty ester of polyhydric alcohol, and containing sulfate radical, derivant (the such as alkylaryl polyglycol ether of the compound of sulfonate radical and phosphate radical, alkylsulfonate, alkyl sulfate, arylsulphonate), protein hydrolysate, lignosulphonic acid bisulfate waste liquor and methylcellulose.If one of one of described active component and/or described inert carrier be insoluble in water and when use be carry out in water time, then the existence of surfactant is necessary.In the ratio of the surfactant scope preferably in 5 to 40 weight % of the compositions of the present invention.

Suitable surfactant (adjuvant, emulsifying agent, dispersant, protective colloid, wetting agent and binding agent) include all common ion-types and nonionisable substance, the nonyl phenol of such as ethoxylation, the polyalkylene glycol ethers of straight or branched alcohol, the product of alkylphenol and oxirane and/or expoxy propane, the product of fatty acid amine and oxirane and/or expoxy propane, also has fatty acid ester, alkylsulfonate, alkyl sulfate, alkyl ether sulfate, alkhyl ethers phosphate, aryl-sulfate, ethoxylated aryl induced by alkyl hydroxybenzene (such as triphenyl vinyl phenol-ethoxylate), also have ethoxylation and propenoxylated aryl alkyl phenols (as sulphation or phosphorylation aryl alkyl phenols-ethoxylate and-ethyoxyl-and propoxylate).Other examples are natural and synthesis water-soluble polymers, such as lignosulfonates, gelatin, Radix Acaciae senegalis, phospholipid, starch, Hydrophobic Modified Starch and cellulose derivative, especially cellulose esters and cellulose ether, also has polyvinyl alcohol, polyvinyl acetate, polyvinylpyrrolidone, polyacrylic acid, the combined polymerization product of polymethylacrylic acid and (methyl) acrylic acid and (methyl) acrylate, and the methacrylic acid neutralized with alkali metal hydroxide and the combined polymerization product of methacrylate, also has the condensation product of optionally substituted naphthalene sulfonate and formaldehyde.

Coloring agent can be used, such as inorganic pigment, for instance ferrum oxide, titanium oxide and Prussian blue, and organic dyestuff, for instance alizarin dyes, azo dye and metallized phthalocyanine dye.

May be present in the defoamer in preparation and include such as silicone emulsion, long-chain alcohol, fatty acid and their salt, and fluoro organic matter matter and mixture thereof.

The example of thickening agent is polysaccharide, for instance xanthan gum or aluminium-magnesium silicate；Silicate, for instance attapulgite, bentonite and particulate silicon dioxide.

If it is suitable, it is possible to there is other extra components, for instance protecting colloid, binding agent, adhesive, thickening agent, thixotroping material, penetrating agent, stabilizer, chelating agen, chelating agent.Generally, active component can combine with any solid being generally used for preparation purpose or liquid additive.

nullThe active agent combinations of the present invention or compositions can itself use，Or according to they specific physically and/or chemically character，Use with their preparation or the type of service prepared by them，Such as aerosol、Capsule suspension liquid、Cold-atomization concentrating agents、Warm-atomization concentrating agents、The granule of cladding、Granula subtilis、Flowable concentrating agents for seed treatment、Ready to use solution、Powder、Cream、Water in oil emulsion、Oil in water emulsion、Bulky grain、Microgranule、Oil dispersible powder、The mixable flowable concentrating agents of oil、Oil miscible liquids、Gas (under stress)、Gas generation product、Foam、Paste、The seed of coated pesticidal、Suspension concentrating agents、Suspension emulsion concentrating agents、Solubility concentrating agents、Suspension、Wettable powder、Soluble powder、Powder and granule、Water soluble and water-dispersible granules or tablet、Water solublity and water-dispersible powder for seed treatment、Wettable powder、With the natural product of active component dipping and synthetic，And also have for seed in polymeric material microencapsulation in coating material，And also have ULV cold-atomization and warm-atomization preparation.

The active agent combinations of the present invention not only includes the preparation namely using and can being applied to by suitable device plant or seed, the commercial concentrating agents of necessary dilute with water before being additionally included in use.Conventional application for such as dilution with water and spray subsequently gained spray solution, use after dilution in oil, do not dilute directly use, the seed treatment of granule or soil application.

The preparation being previously mentioned can be prepared in a way known, such as by by active component and the filler of at least one routine, solvent or diluent, adjuvant, emulsifying agent, dispersant and/or binding agent or fixative, wetting agent, waterproofing agent, if it is suitable, also have desiccant and UV stabilizer, if and properly, also having coloring agent and pigment, defoamer, preservative, inorganic and organic thickening agent, binding agent, gibberellins and other processing aids and water mixing to prepare.According to preparation type to be prepared, other procedure of processings are necessary, for instance, wet grinding, dry grinding and pelletize.

The active agent combinations of the present invention form own can exist or exist with the dosage form of they (commercially) and exist using the type of service prepared as the mixture with the active component of other (known) by these preparations, other active component described such as insecticide, attractant, biocide, bactericide, acaricide, nematicide, antifungal, growth regulator, herbicide, fertilizer, safener and/or semiochemical.

Adopt active component or the compositions of the present invention are made directly or act on their environment, habitat or storage space by conventional treatment method and carry out, described conventional treatment method such as by dipping, spraying, atomization, pouring, evaporation, dusting, atomizing, spread fertilizer over the fields, foam, be coated with, be coated with, water (dipping), drip irrigation, and for propagating materials (especially for seed), process also by dry seeds, wet seed treatment, serosity process, encrustment, with one or more layers coating coating etc..Use active component also by ultra-low-volume method or active agent preparation or active component self are injected in soil.

The active agent combinations of the present invention or compositions demonstrate effective microbiocidal activity and can crop protection and in material protection for preventing and treating unwanted microorganism, such as fungus and antibacterial.

The invention still further relates to the method for preventing and treating unwanted microorganism, it is characterised in that the active ingredient combination of the present invention or compositions are put on plant pathogenic fungi, plant pathogenetic bacteria and/or its habitat.

Antifungal can be used for preventing and treating plant pathogenic fungi in crop protection.Antifungal is characterized by the remarkable efficacy of antagonism broad-spectrum plant pathogenic fungi, described plant pathogenic fungi includes soilborne pathogen, particularly Plasmodiophoromycetes (Plasmodiophoromycetes), Oomycete (Peronosporomycetes) (synonym: Oomycete (Oomycetes)), Chytridiomycetes (Chytridiomycetes), Zygomycetes (Zygomycetes), Ascomycetes (Ascomycetes), the member of Basidiomycetes (Basidiomycetes) and deuteromycetes (Deuteromycetes) (synonym: deuteromycetes (Fungiimperfecti)).Some antifungal have systemic activity and can as blade, seed pelleting or soil fungicide in plant protection.Additionally, they are suitable for preventing and treating fungus, particularly invade and harass the timber of plant or the fungus of root.

Bactericide can be used for preventing and treating pseudomonadaceae (Pseudomonadaceae), Rhizobiaceae (Rhizobiaceae), enterobacteriaceae (Enterobacteriaceae), Corynebacteriaceae (Corynebacteriaceae) and Streptomycetaceae (Streptomycetaceae) in crop protection.

Can include according to the limiting examples of the pathogen of the fungal disease that the present invention processes:

The disease caused by powdery mildew pathogen, described powdery mildew pathogen such as, plant, for instance grass family dlumeria graminis (Blumeriagraminis) by Blumeria (Blumeria)；Podosphaera (Podosphaera) is planted, such as Fructus Mali pumilae powdery mildew (Podosphaeraleucotricha)；Sphaerotheca (Sphaerotheca) is planted, such as cucumber powdery mildew fungus (Sphaerothecafuliginea)；Uncinula (Uncinula) is planted, such as grape powdery mildew (Uncinulanecator)；

The disease caused by rust pathogen, described rust pathogen such as, plant, for instance pears rest fungus (Gymnosporangiumsabinae) by Gymnosporangium (Gymnosporangium)；Camel spore Rust (Hemileia) is planted, for instance coffee rest fungus (Hemileiavastatrix)；Phakopsora (Phakopsora) is planted, for instance Phakopsora pachyrhizi (Phakopsorapachyrhizi) and Herba podocarpii oxyphylli layer rest fungus (Phakopsorameibomiae)；Puccinia (Puccinia) is planted, for instance Puccinia recondita f. sp. tritici (Pucciniarecondite), puccinia triticinia (P.triticina), puccinia graminis (P.graminis) or puccinia striiformis (P.striiformis)；Uromyces (Uromyces) is planted, for instance wart top monospore rest fungus (Uromycesappendiculatus)；

The disease caused by Oomycete (Oomycetes) pathogen, described pathogen such as Albugo (Albugo) is planted, for instance white rust pathogenic bacteria (Albugocandida)；Bremia (Bremia) is planted, for instance Caulis et Folium Lactucae sativae dish stalk mycete (Bremialactcae)；Peronospora (Peronospora) is planted, for instance Semen Pisi sativi downy mildew (Peronosporapisi) or Semen Brassicae Campestris downy mildew (P.brassicae)；Phytophthora (Phytophthora) is planted, for instance phytophthora infestans (Phytophthorainfestans)；Plasmopara (Plasmopara) is planted, for instance Fructus Vitis viniferae single shaft mould (Plasmoparaviticola)；Pseudoperonospora (Pseudoperonospora) is planted, for instance the false downy mildew (Pseudoperonosporahumuli) of grass or bacterium of downy mildew of cucumber (Pseudoperonosporacubensis)；Rotten mold genus (Pythium) is planted, for instance Pythium ultimum bacterium (Pythiumultimum)；

Sick by the leaf spot that such as following pathogen causes and leaf withering: Alternaria (Alternaria) is planted, for instance alternaria solani (Alternariasolani)；Cercospora (Cercospora) is planted, for instance pathogen Radix Betae tail spore bacterium (Cercosporabeticola)；Cladosporium (Cladiosporium) is planted, for instance Fructus Cucumidis sativi Fulvia fulva Ciferri (Cladiosporiumcucumerinum)；Cochliobolus belongs to (Cochliobolus) and plants, such as standing grain cochliobolus (Cochliobolussativus) (conidial form: Drechslera (Drechslera), synonym: Helminthosporium (Helminthosporium)), Oryza sativa L. cochliobolus (Cochliobolusmiyabeanus)；Hair disc spore belongs to (Colletotrichum) and plants, for instance bean colletotrichum (Colletotrichumlindemuthanium)；Oleaginea belongs to (Cycloconium) and plants, for instance Fructus oleae europaeae peafowl plaque (Cycloconiumoleaginum)；Between seat shell belong to (Diaporthe) plant, for instance Citrus brown blossom-end rot bacterium (Diaporthecitri)；Elsinoe (Elsinoe) is planted, for instance the mandarin orange Elsinochrome (Elsinoefawcettii) of shot hole；The long spore of dish belongs to (Gloeosporium) and plants, for instance peach tree rotten pathogenic bacteria (Gloeosporiumlaeticolor)；Small cluster shell belongs to (Glomerella) and plants, for instance anthrax bacteria (Glomerellacingulata)；Ball seat Pseudomonas (Guignardia) is planted, for instance Fructus Vitis viniferae Guignardia (Guignardiabidwelli)；Leptosphaeria (Leptosphaeria) is planted, for instance Cruciferae ball cavity bacteria (Leptosphaeriamaculans), clever withered shell ball cavity bacteria (Leptosphaerianodorum)；Between seat shell belong to (Magnaporthe) plant, for instance Pyricularia oryzae (Magnaporthegrisea)；Micro-tuberosity Pseudomonas (Microdochium) is planted, for instance avenge the withered bacterium of mould leaf (Microdochiumnivale)；Mycosphaerella (Mycosphaerella) is planted, for instance standing grain green-ball chamber bacterium (Mycosphaerellagraminicola), Semen arachidis hypogaeae spherical cavity bacterium (M.arachidicola) and Fiji spherical cavity bacterium (M.fijiensis)；Mycosphaerella (Phaeosphaeria) is planted, for instance wheat leaf blight bacterium (Phaeosphaerianodorum)；Nuclear cavity Pseudomonas (Pyrenophora) is planted, for instance circle nuclear cavity bacteria (Pyrenophorateres), couchgrass nuclear cavity bacteria (Pyrenophoratriticirepentis)；Ramularia (Ramularia) plant, for instance post every spore leaf spot fungi (Ramulariacollo-cygni), white macula post every spore (Ramulariaareola)；Beak genus (Rhynchosporium) is planted, for instance barley leaf blotch bacterium (Rhynchosporiumsecalis)；Septoria (Septoria) is planted, for instance Herba Apii graveolentis Septoria musiva (Septoriaapii), Fructus Lycopersici esculenti Septoria musiva (Septorialycopersii)；Core coral Pseudomonas (Typhula) is planted, for instance meat spore core coral bacterium (Typhulaincarnata)；Venturia (Venturia) is planted, for instance venturia inaequalis (Venturiainaequalis)；

Disease by the root that such as following pathogen causes and stem: corticium (Corticium) is planted, for instance standing grain photovoltaicing leather bacteria (Corticiumgraminearum)；Fusarium (Fusarium) is planted, for instance Fusarium oxysporum (Fusariumoxysporum)；Gaeumannomyce belongs to (Gaeumannomyces) and plants, for instance gaeumannomyces graminis (Gaeumannomycesgraminis)；Rhizoctonia (Rhizoctonia) is planted, such as, for instance Rhizoctonia solani Kuhn (Rhizoctoniasolani)；Broom Thamnidium (Sarocladium) disease such as caused by rice broom branch mould (Sarocladiumoryzae)；Sclerotium (Sclerotium) disease such as caused by Sclerotium oryzae (Sclerotiumoryzae)；Tapesia belongs to kind, for instance Tapesiaacuformis；Thiclaviopsis (Thielaviopsis) is planted, for instance black root of tobacco bacterium (Thielaviopsisbasicola)；

By the spadix that such as following pathogen causes and panicle disease (including corncob): Alternaria (Alternaria) is planted, for instance Alternaria kind (Alternariaspp.)；Eurotium (Aspergillus) is planted, for instance Aspergillus flavus (Aspergillusflavus)；Cladosporium (Cladosporium) is planted, for instance Cladosporium cladosporioides (Cladosporiumcladosporioides)；Claviceps (Claviceps) is planted, for instance purple Clavicipitaceae (Clavicepspurpurea)；Fusarium (Fusarium) is planted, for instance fusarium culmorum (Fusariumculmorum)；Gibberella (Gibberella) is planted, for instance Gibberella zeae (Gibberellazeae)；Little setting-out shell belongs to (Monographella) and plants, for instance avenge rotten little setting-out shell (Monographellanivalis)；Septoria (Septoria) is planted, for instance the Semen Tritici aestivi withered Septoria musiva of grain husk (Septorianodorum)；

The disease caused by smut, described smut such as axle Ustilago (Sphacelotheca) is planted, for instance Sporisorium reilianum (Sphacelothecareiliana)；Tilletia (Tilletia) is planted, for instance bunt of wheat bacterium (Tilletiacaries), T. contraversa (T.controversa)；Urocystis (Urocystis) is planted, for instance rye (Secale cereale L.) bar black tassel bacteria (Urocystisocculta)；Ustilago (Ustilago) is planted, for instance naked smut (Ustilagonuda), ustilago (U.nudatritici)；

Fruit rot by such as following pathogen causes: Eurotium (Aspergillus) is planted, for instance Aspergillus flavus (Aspergillusflavus)；Botrytis (Botrytis) is planted, for instance ash arrhizus bacteria (Botrytiscinerea)；Penicillium (Penicillium) is planted, for instance penicillium expansum (Penicilliumexpansum), penicillium purpurogenum (P.purpurogenum)；Sclerotinia (Sclerotinia) is planted, for instance sclerotinite (Sclerotiniasclerotiorum)；Verticillium (Verticilium) is planted, for instance black and white wheel branch spore (Verticiliumalboatrum)；

By such as following pathogen cause plant pass and soil pass corruption, mouldy, wilt, rot and disease of dampinging off: Alternaria (Alternaria) plant, for instance caused by Alternaria brassicicola (Alternariabrassicicola)；Aphanomyces (Aphanomyces) is planted, for instance caused by root-rot silk capsule mould (Aphanomyceseuteiches)；Ascochyta (Ascochyta) is planted, for instance caused by crystalline lens shell two spore (Ascochytalentis)；Eurotium (Aspergillus) is planted, for instance caused by Aspergillus flavus (Aspergillusflavus)；Cladosporium (Cladosporium) is planted, for instance caused by branch sporozoite bacterium (Cladosporiumherbarum)；Cochliobolus (Cochliobolus) is planted, for instance caused by standing grain cochliobolus (Cochliobolussativus)；(conidial form: Drechslera (Drechslera), Bipolaris (Bipolaris), synonym: Helminthosporium (Helminthosporium))；Hair disc spore belongs to (Colletotrichum) and plants, for instance caused by ball anthrax (Colletotrichumcoccodes)；Fusarium (Fusarium) is planted, for instance caused by Fusarium (Fusariumculmorum)；Gibberella (Gibberella) belongs to be planted, for instance caused by Gibberella zeae (Gibberellazeae)；Sub-big Phoma (Macrophomina) is planted, for instance caused by Kidney bean shell ball spore bacterium (Macrophominaphaseolina)；Little setting-out shell belongs to (Monographella) and plants, for instance by avenging what rotten little setting-out shell (Monographellanivalis) caused；Penicillium (Penicillium) is planted, for instance caused by penicillium expansum (Penicilliumexpansum)；Phoma (Phoma) is planted, for instance caused by the black shin Phoma sp (Phomalingam) of cause of disease；Phomopsis (Phomopsis) is planted, for instance caused by Semen sojae atricolor Phomopsis (Phomopsissojae)；Phytophthora (Phytophthora) is planted, for instance caused by Phytophthora cactorum (Phytophthoracactorum)；Nuclear cavity Pseudomonas (Pyrenophora) is planted, for instance caused by wheat and barley nuclear cavity bacteria (Pyrenophoragraminea)；Pyricularia Sacc. (Pyricularia) is planted, for instance caused by Pyricularia oryzae (Pyriculariaoryzae)；Rotten mold genus (Pythium) is planted, for instance caused by Pythium ultimum (Pythiumultimum)；Rhizoctonia (Rhizoctonia) is planted, for instance caused by Rhizoctonia solani Kuhn (Rhizoctoniasolani)；Rhizopus (Rhizopus) is planted, for instance caused by rhizopus oryzae (Rhizopusoryzae)；Bulbil belongs to (Sclerotium) and plants, for instance caused by Sclerotium rolfsii (Sclerotiumrolfsii)；Septoria (Septoria) is planted, for instance caused by the Semen Tritici aestivi withered Septoria musiva of grain husk (Septorianodorum)；Core coral Pseudomonas (Typhula) is planted, for instance caused by meat spore core coral bacterium (Typhulaincarnata)；Verticillium (Verticillium) is planted, for instance caused by verticillium dahliae (Verticilliumdahliae)；

By the cancer that such as following pathogen causes, insect gall and witches' broom (witches ' broom) disease: Nectria (Nectria) is planted, for instance the red shell bacterium (Nectriagalligena) of cancer clump done by a kind of fruit, such as apple, pear, etc.；

Wilting disease by such as following pathogen causes: chain sclerotinia sclerotiorum belongs to (Monilinia) and plants, for instance drupe chain sclerotinia sclerotiorum (Monilinialaxa)；

By the leaf blister that such as following pathogen causes or leaf curl disease, Exobasidium (Exobasidium) is planted, for instance corrupted outer cross-arming bacteria (Exobasidiumvexans)；

Exoascus (Taphrina) is planted, for instance deformity external capsule bacterium (Taphrinadeformans)；

The degeneration disease of xylophyta: by the Esca disease that such as rhizopus lattice spore bacterium (Phaemoniellaclamydospora), Phaeoacremoniumaleophilium and Fomitiporiamediterranea cause；The eutypa dieback (Eutypadyeback) that (Eutypalata) causes is belonged to by such as lateral bending spore shell；By Ganoderma (Ganoderma) disease that such as island Ganoderma (Ganodermaboninense) causes；Rigidoporus (Rigidoporus) disease caused by such as Rigidoporus lignosus (Rigidoporuslignosus)；

Disease by the flower that such as following pathogen causes and seed: Botrytis (Botrytis) is planted, for instance Botrytis cinerea (Botrytiscinerea)；

Plant tuber disease by such as following pathogen causes: Rhizoctonia (Rhizoctonia) is planted, for instance Rhizoctonia solani Kuhn (Rhizoctoniasolani)；Helminthosporium (Helminthosporium) is planted, for instance Helminthosporium solani (Helminthosporiumsolani)；

Clubroot disease by such as following pathogen causes: knee Pseudomonas (Plasmodiophora) is planted, for instance Caulis et Folium Brassicae campestris plasmodiophora brassicae (Plamodiophorabrassicae)；

The disease caused by bacterial pathogen, described bacterial pathogen such as, plant, for instance rice Xanthomonas campestris bacterial leaf spot mutation (Xanthomonascampestrispv.oryzae) by yellow unit cell (Xanthomonas)；False unit cell (Pseudomonas) is planted, for instance pseudomonas syringae Fructus Cucumidis sativi pvs oryzae and oryzicola (pseudomonassyringaepv.lachrymans)；Ou Wenshi (Erwinia) plants, for instance bite Erzvinia amylovora (Erwiniaamylovora).

Preferably can prevent and treat following soybean diseases:

nullIt is positioned at leaf by what such as following pathogen caused、Stem、Fungal disease on pod and seed，Such as Alternaria leaf spot (Alternarialeafspot) (Alternariaspec.atranstenuissima)、Anthrax (Colletotrichumgloeosporoidesdematiumvar.truncatum)、Brown spot (Semen sojae atricolor septoria musiva (Septoriaglycines))、Cercospora leaf spot and leaf blight (Cercosporaleafspotandblight) (Kikuchi tail spore (Cercosporakikuchii))、Choanephora (Choanephora) leaf blight (funnel hairpin mould (Choanephorainfundibuliferatrispora (Syn.))、Dactuliophora leaf spot (Dactuliophoraglycines)、Downy mildew (downymildew) (northeast downy mildew (Peronosporamanshurica))、Drechslera (drechslera) droop (Drechsleraglycini)、Frog eye leaf spot (Semen sojae atricolor tail spore (Cercosporasojina))、Little bare hull belongs to (leptosphaerulina) leaf spot (the little bare hull of Herba Trifolii Pratentis (Leptosphaerulinatrifolii))、Phyllosticta (phyllostica) leaf spot (the raw phyllosticta (Phyllostictasojaecola) of Semen sojae atricolor)、Pod and stem droop (Semen sojae atricolor Phomopsis (Phomopsissojae))；nullPowdery mildew (diffusion cross hair shell (Microsphaeradiffusa))、Pyrenochaeta (pyrenochaeta) leaf spot (Pyrenochaetaglycines)、Rhizoctonia aerial parts、Leaf blight and damping-off (Rhizoctonia solani Kuhn (Rhizoctoniasolani))、Rust (Phakopsora pachyrhizi (Phakopsorapachyrhizi)、Herba podocarpii oxyphylli layer rest fungus (Phakopsorameibomiae))、Scall (Semen sojae atricolor crust circle spore (Sphacelomaglycines))、Stemphylium (stemphylium) leaf blight (handle of crawling mould (Stemphyliumbotryosum))、Target spot (targetspot) (Corynespora cassicola (Corynesporacassiicola)).

nullFungal disease by the root that such as following pathogen causes and basal part of stem，Such as black root rot (blackrootrot) (Calonectriacrotalariae)、Charcoal rot (raw shell ball spore (Macrophominaphaseolina) of Kidney bean)、Fusarium droop or wilting、Root rot and pod and collar rot ((point sickle spore (Fusariumoxysporum)、Straight beak sickle spore (Fusariumorthoceras)、F.semitectum (Fusariumsemitectum)、Herba Equiseti Hiemalis's sickle spore (Fusariumequiseti))、Mycoleptodiscus root rot (Mycoleptodiscusterrestris)、Neocosmospora (neocosmospora) (invades the new red shell (Neocosmopsporavasinfecta) of villous themeda)、Pod and stem wilt (between Kidney bean seat shell (Diaporthephaseolorum))、Stem canker (Semen sojae atricolor north stem canker (Diaporthephaseolorumvar.caulivora))、Phytophthora maize ear rot (big male epidemic disease mould (Phytophthoramegasperma))、Brown stem rot (brown stem rot bacterium (Phialophoragregata))、(the melon and fruit corruption mould (Pythiumaphanidermatum) of rotten mold genus maize ear rot、Abnormal female corruption mould (Pythiumirregulare)、Pythium debaryanum (Pythiumdebaryanum)、P. myriotylum (Pythiummyriotylum)、Pythium ultimum (Pythiumultimum))、Rhizoctonia root rot、Stem rot and damping off (Rhizoctonia solani Kuhn (Rhizoctoniasolani))、Sclerotinite stem rot (sclerotinite (Sclerotiniasclerotiorum))、Sclerotinite southern blight (Sclerotiniarolfsii)、Thiclaviopsis root rot (thielaviopsis sp (Thielaviopsisbasicola)).

The active agent combinations of the present invention or compositions can be used for therapeutic or protectiveness/prophylactically prevent and treat phytopathogenic fungi.Therefore; the invention still further relates to the active agent combinations of the application of the invention or compositions and prevent and treat therapeutic and the protectiveness method of phytopathogenic fungi, wherein the active agent combinations of the present invention or compositions are put on the soil of seed, plant or plant parts, fruit or plant growing.

Described active component has a good plant tolerance under the concentration needed for controlling plant diseases, the fact that so that it can process the aerial parts of plant, the root stock of breeding and seed and soil.

All of plant and plant parts can be processed according to the present invention.Plant means all of plant and plants genus population, for instance need and unwanted wild plant, cultigen and botanical variety (regardless of whether by rights protection of plant variety or plant breeder).Cultigen and botanical variety can be through conventional breeding and breeding method or the plant obtained by biological engineering and gene engineering method, the breeding of described routine and breeding method can be assisted by one or more biological technique methods or supplement, for instance by using dihaploid, protoplast fusion, random and site directed mutagenesis, molecule or genetic marker.Plant parts means all of ground of plant and under ground portion and organ, for instance bud, leaf, Hua Hegen, lists such as leaf, needle, stem, branch, flower, sporophore, fruit and seed and root, bulb and rhizome accordingly.Crop and asexual and case of propagation material, for instance cutting, bulb, rhizome, stolon and seed fall within plant parts.

The active agent combinations of the present invention or compositions have favourable Homoiotherm toxicity and can be tolerated completely by environment, it is adaptable to protection plant and plant organ, gather yield for raising, improve the quality of material of gathering.Preferably use them as crop production compositions.They are to general sensitive and to have the species of resistance active, and to all of or some stages of development are active.

The active agent combinations of the present invention is also applied for the seed of any plant variety that protection uses in agricultural, greenhouse, forest or gardening and vinegrowing.

nullFollowing major crop plants can be included: Semen Maydis according to the plant that the present invention carries out processing、Semen sojae atricolor、Herba Medicaginis、Cotton Gossypii、Helianthi、Btassica (Brassica) oilseed (such as colea (Brassicanapus) (such as Semen Brassicae Campestris、Semen Brassicae campestris)、Overgrown with weeds blue or green (Brassicarapa)、Caulis et Folium Brassicae junceae (B.juncea) (such as (field) Semen Sinapis) and brassicacarinata (Brassicacarinata))、Arecaceae kind (Arecaceaesp.) (such as Elaeis guineensis Jacq.，Cortex cocois radicis)、Rice、Semen Tritici aestivi、Radix Betae、Caulis Sacchari sinensis、Herba bromi japonici、Rye (Secale cereale L.)、Fructus Hordei Vulgaris、Foxtail millet and Sorghum vulgare Pers.、Triticale hexaploide Lart.、Caulis et Folium Lini、Nut、Fructus Vitis viniferae and liana and the various fruits and vegetables from various plant classification units，Such as Rosaceae kind (Rosaceaesp.) is (such as pip fruit (such as Fructus Mali pumilae and pears)，Also has stone fruit (such as Fructus Pruni、Fructus Pruni pseudocerasi、Almond、Fructus Pruni salicinae and Fructus Persicae)，And berry fruits is (such as Fructus Fragariae Ananssae、Fructus Rubi、Red gallon and cassis and gooseberry))、Fructus Camelliae sinensis section kind (Ribesioidaesp.)、Juglandaceae kind (Juglandaceaesp.)、Betulaceae kind (Betulaceaesp.)、Anacardiaceae kind (Anacardiaceaesp.)、Fagaceae kind (Fagaceaesp.)、Moraceae kind (Moraceaesp.)、Oleaceae kind (Oleaceaesp.) (such as Fructus Canarii albi)、Actinidiaceae kind (Actinidaceaesp.)、Lauraceae kind (Lauraceaesp.) (such as avocado、Cortex cinnamomi japonici (Ramulus Cinnamomi)、Camphora)、Musaceae kind (Musaceaesp.) (such as Banana tree and banana plantation)、Rubiaceae kind (Rubiaceaesp.) (such as coffee)、Theaceae kind (Theaceaesp.) (such as tea)、Sterculiaceae kind (Sterculiceaesp.)、Rutaceae kind (Rutaceaesp.) (such as Fructus Citri Limoniae、Orange、Fructus Citri tangerinae and grapefruit)；nullSolanaceae kind (Solanaceaesp.) is (such as，Fructus Lycopersici esculenti、Rhizoma Solani tuber osi、Fructus Piperis、Fructus Capsici、Fructus Solani melongenae、Nicotiana tabacum L.)、Liliaceae kind (Liliaceaesp.)、Compositae kind (Compositaesp.) (such as Caulis et Folium Lactucae sativae、Carlina acaulis and Herba Cichorii-include root Herba Cichorii、Herba Cichorii or common Herba Cichorii)、Umbelliferae kind (Umbelliferaesp.) (such as Radix Dauci Sativae、Parsley、Herba Apii graveolentis and celeriac)、Cucurbitaceae kind (Cucurbitaceaesp.) (such as Fructus Cucumidis sativi-include gherkin、Cucurbita pepo L.、Citrullus vulgaris、Calabash and Fructus Chaenomelis)、Herba Alii fistulosi section kind (Alliaceaesp.) (such as fragrant-flowered garlic、Bulbus Allii Cepae)、Cruciferae kind (Cruciferaesp.) (such as white Caulis et Folium Brassicae capitatae、Red cabbage、Broccoli、Brassica oleracea L. var. botrytis L.、Brussels sprout、Plantula Brassicae chinensis、Kohlrabi、Radix Raphani、Wasabia japonic (Euterma Wasabi)、Herba Oenanthes Javanicae and Chinese cabbage)、Pulse family kind (Leguminosaesp.) (such as Semen arachidis hypogaeae、Semen Pisi sativi、Seem Lablab Album and Kidney bean such as Semen Canavaliae and Semen Viciae fabae)、Chenopodiaceae kind (Chenopodiaceaesp.) (such as Swiss chard、Fodder beet、Herba Spinaciae、Beet root)、Flax family (Linaceae) kind (Linaceaesp.) (such as Fructus Cannabis)、Cannabaceae kind (Cannabeaceasp.) (such as ganja)、Malvaceae kind (Malvaceaesp.) (such as Abelmoschus esculentus、Cocoa)、Papaveraceae (Papaveraceae) (such as Semen Papaveris)、Liliaceae (Asparagaceae) (such as Germinatus Phragmitis)；Useful plant in gardening and forest and ornamental plant, including meadow, lawn, herbage and Folium Stevlae Rebaudianae (Steviarebaudiana)；And the respective genetic modification type of these plants.

The active agent combinations of the present invention also shows effective invigoration effect in plant.Therefore, they can be used for adjusting vegeto-animal system of defense to resist the invasion and attack from less desirable microorganism.

Plant strengthening (induction of resistance) material is understood to mean that in the present context can those materials of activated plant defense system by this way: when inoculating less desirable microorganism subsequently, treated plant demonstrates the highly resistant to these microorganisms.

The active agent combinations of the present invention is suitable for improving the yield of crop.

Additionally, in the context of the present invention, plant physiology effect includes herein below:

The toleration of abiotic stress, including temperature tolerance, recovery after drought tolerance and drought stress, water application efficiency (water consumption corresponding to reducing), flood toleration, ozone stress and ultraviolet toleration, to the toleration as chemicals such as heavy metal, salt, pesticide (safener).

The toleration of biotic, including the resistance of the antagonism nematicide of the fungus resistant increased and increase, virus and antibacterial.In the context of the present invention, biotic toleration preferably includes the fungus resistant of raising and the resistance of the antagonism nematicide of increase.

The plant vigor (including plant health/plant quality and seed vitality) improved, the thribble failure of minimizing, the outward appearance of improvement, the recovery of enhancing, the afforestation effect of improvement and the photosynthetic efficiency of raising.

Effect to phytohormone and/or functional enzyme.

Effect to growth regulator (accelerator), including rudiment earlier, better emerge, more flourishing root system and/or the root growth of improvement, the tillering ability strengthened, more efficient tiller, more prematurity, the plant height increased and/or Biomass, the stem shortened, at shoot growth, core/spike number order, the number of the fringe of every square metre, the number of stolon and/or the improvement of flower number aspect, the harvest index improved, bigger blade, less dead basal leaf, the phyllotaxy improved, ripe/fruit result earlier earlier, uniform ripe, the Grain filling duration improved, better fruit fineness, bigger fruit/vegetable size, the lodging of germinating resistance and minimizing.

The yield increased, refers to the product qualities of the total biomass of per hectare, the yield of per hectare, core/fruit weight, seed size and/or hectolitre weight and improvement, including:

The processability improved, relate to size distribution (core, fruit etc.), uniform maturation, grain moisture, better powder process, better wine brewing, better brewage, the juice productivity increased, gathering property, digestibility, sedimentation value, drop value, pod stability, storage stability, fibre length/intensity/the uniformity improved, the milk of the animal that the ensilage of increase is fed and/or the quality of meat, it is adaptable to culinary art is with fried；

Also include the marketability improved, relate to the quality of fruit/corn of improvement, size distribution (core, fruit etc.), the storage/shelf-life increased, hardness/softness, taste (fragrance, quality etc.), grade (size, shape, berry number etc.), often a string berry/fruit number, brittleness, freshness, use wax coverage, the frequency of physiological disorder, color etc.；

Also include the desired constituents increased, for instance protein content, fatty acid, oil content, oil quality, aminoacid composition, sugar content, acid content (pH), sugar/acid ratio rate (Brix Scale), polyphenol, content of starch, nutrient status, glutelin content/index, energy content, taste etc.；

And also include the less desirable composition reduced, for instance less fungimycin, less aflatoxin, geosmin level, the fragrance of phenol, laccase, polyphenol oxidase and peroxidase, nitrate content etc..

Sustainable agriculture, including nutrient utilization ratio, especially nitrogen (N)-utilization ratio, phosphorus (P)-utilization ratio, WUEL, improve transpiration, breathing and/or CO₂Assimilation rate, better dross, the calcium metabolism etc. of improvement.

The aging postponed, improvement including the plant physiology manifested, for instance, in the longer Grain Filling stage, cause higher yield, that the greenery of plant color duration is longer, and therefore also includes color (greening), water content, aridity etc..Therefore, in the context of the present invention, oneself finds that using of the active agent combinations of the concrete present invention allows to extend the greenery area persistent period, the maturation (aging) of its delay plant.Being the longer Grain Filling stage to the principal benefits of peasant, it causes higher yield.Peasant be another benefit is that greater flexibility on harvest time.

Wherein " sedimentation value " is measuring of protein quality, and according to Zeleny (Zeleny value), sedimentation value describes the degree of the flour sedimentation being suspended in lactic acid solution during the interval of standard.This is considered measuring of baking quality.The gluten fraction of the flour swelling rate of settling affecting flour suspension in lactic acid solution.Higher glutelin content and better qualities of gluten result in slower sedimentation and higher Zeleny test value.The sedimentation value of flour depends on that wheat protein forms, and main relevant to the volume of protein content, wheat hardness and pan and the kitchen range top.Compared with SDS settling volume, association higher between loaf volume and Zeleny settling volume is likely due to protein content, and affect volume and Zeleny value the two is caused (CzechJ.FoodSci. the 21st volume, the 3rd phase: 91 96,2000).

Additionally, " Falling Number " as referred to herein is measuring of the baking quality of frumentum particularly Semen Tritici aestivi.Falling Number test shows, it is possible to oneself is through bud damage occurs.Oneself there occurs change to this means the physical property of the Starch Fraction in little wheat core.Wherein, Falling Number instrument sticks with paste the resistance to landing plunger and analysis of viscosity by measuring flour and the water surface.The time (in seconds) of its generation is called Falling Number.Falling Number result is registered as in Semen Tritici aestivi or flour sample the index of enzymatic activity, and result represented with second time.High Falling Number (such as, higher than 300 seconds) shows minimum enzymatic activity and colory Semen Tritici aestivi or flour.Low Falling Number (such as, lower than 250 seconds) shows significant enzymatic activity and the impaired Semen Tritici aestivi of bud or flour.

Term " more flourishing root system "/" root growth of improvement " refers to longer root system, deeper of root growth, faster root growth, higher root dry weight/fresh weight, higher root volume, bigger root surface area, bigger root diameter, higher root stability, more root branch, the root hair of higher amount and/or the more tip of a root, and can pass through to adopt suitable method and image analysis program (such as WinRhiz) analyze root and construct and measure.

Term " crop water application efficiency " refers to the agricultural amount that the per unit water consumed produces technically, refer to the value of the product of the per unit water volume generation consumed economically, and can such as measure in the way of the quantity of the yield of per hectare, the Biomass of plant, thousand nuclear masses and every square metre of fringe.

Term " nitrogen use efficiency " refers to the agricultural amount that the per unit nitrogen consumed produces technically, refers to the value of the product of the per unit nitrogen generation consumed economically, and reflection absorbs and utilization ratio.

The color of improvement/improvement of greening and the delay of the photosynthetic efficiency of improvement and aging can adopt widely-known technique such as HandyPea system (Hansatech) to measure.Fv/Fm is widely used in the parameter of the maximum quantum efficiency of instruction photosynthetical system II (PSII).This parameter is widely regarded as the selectivity indication of plant photosynthesis performance, adopts healthy sample generally can realize the maximum Fv/Fm value of about 0.85.If sample oneself be exposed to some type of biology or abiotic stress factor (described factor oneself reduce the photochemical quenching ability of the energy in PSII), then it will be observed that lower than this value.Fv/Fm represents the variable fluorescence (Fv) ratio relative to maximum fluorescence value (Fm).This performance index is substantially that the index of sample vigor is (referring to such as AdvancedTechniquesinSoilMicrobiology, 2007,11,319-341；AppliedSoilEcology, 2000,15,169-182.).

The color of improvement/improvement of greening and the delay of the photosynthetic efficiency of improvement and aging can also by measuring net photosynthetic rate (Pn), measure chlorophyll content (such as by the method for extracting pigment of Ziegler and Ehle), measure photochemical efficiency (Fv/Fm ratio), measure shoot growth and final root and/or hat Biomass, measuring tiller density and root mortality rate and be estimated.

In the context of the present invention, it is preferable that improve selected from following plant physiology effect: the yield of the root system of the root growth of enhancing/more flourishing, the greening of improvement, the water application efficiency (relevant to the water consumption reduced) of improvement, the nutrient utilization ratio (including nitrogen (N) utilization ratio particularly improved) improved, the aging of delay and enhancing.

In the yield strengthened, it is preferable that refer to that the improvement of the improvement of sedimentation value and Falling Number and protein and sugar content is especially for the plant selected from corn (preferably wheat).

Preferably, the new application of the Fungicidal composition of the present invention relates to a) preventative when being with or without resistance management and/or therapeutic ground controlling pathogenic fungi by means and/or nematicide, with the purposes of combination at least one in root growth b) strengthened, the greening of improvement, the water application efficiency of improvement, the aging of delay and the yield of enhancing.From b) group, it is particularly preferred to the root system of enhancing, water application efficiency and nitrogen use efficiency.

The active agent combinations of the present invention or compositions are also applied for processing seed.The major part infringement of the crop plants caused by harmful organism is to be caused by seed infection during storing or after planting and during plant germination and after germinateing.Owing to root and the bud of the plant in growth are especially sensitive, therefore this stage is particularly critical, even if small damage is likely to causes Plant death.Therefore, by using suitable compositions protection seed and germination plant to cause the very big concern of people.

It is known for a long time for preventing and treating phytopathogenic fungi by process plant seed, and is the theme updated.But, seed treatment brings series of problems, and these problems usually can not solve in a satisfactory manner.Therefore, it is desirable to the method for exploitation protection seed and germination plant, the method is saved or is at the least, significantly reduced in after planting or plant germination ensuing crop protects additionally using of compositions.It is also desirable that the amount of the active component that optimization uses thinks that seed and germination plant provide the best invasion and attack protectioning from phytopathogenic fungi, and plant itself is not caused damage by the active component used.Especially, the method processing seed is also considered as the fungicide performance that transgenic plant is intrinsic, to adopt minimal amount of crop production compositions to realize the best protection to seed and germination plant.

Therefore, the invention still further relates to the method by adopting the active agent combinations of the present invention or compositions-treated seed to protect seed and germination plant to attack from phytopathogenic fungi.The invention still further relates to the active agent combinations of the present invention or compositions for processing the purposes that seed is attacked from phytopathogenic fungi to protect seed and germination plant.The invention still further relates to through the active agent combinations of the present invention or compositions-treated to protected from the seed of phytopathogenic fungi invasion and attack.

The preventing and treating of the phytopathogenic fungi damaging plant after Seedling is carried out mainly through adopting crop production compositions to process the position, ground of soil and plant.In view of on the crop production compositions concern on the healthy issuable impact of environment and human and animal, therefore make great efforts to reduce the consumption of active component.

One of advantages of the present invention is; the active agent combinations of the present invention or the special interior absorption of compositions can mean that the described active component of employing and compositions-treated seed not only protect seed self, and the plant also obtained after protection rudiment is from the invasion and attack of phytopathogenic fungi.Therefore, can save at seeding time or after planting soon the direct of crop be processed.

Being also regarded as advantageously, the active agent combinations of the present invention or compositions also can be particularly used for transgenic plant and/or seed, wherein said plant or the plant grown up to by described seed can express the protein of opposing insect.Some insect can be prevented and treated by seed (only expressing protein, for instance insecticidal proteins) described in the active agent combinations of the employing present invention or compositions-treated.Unexpectedly, cooperative effect also being can be observed in this case, this cooperative effect increases the effectiveness of the protective effect of antagonism pest attacks further.

Also describing hereinafter, the active agent combinations of the employing present invention or compositions-treated transgenic plant or seed are particular importances.This seed relating to comprising the plant of at least one heterologous gene.The definition of suitable heterologous gene and example will be provided below.

In the context of the present invention, the active agent combinations of the present invention or compositions individually or are applied to seed in suitable preparation.Preferably, under the sufficiently stable state of seed, seed is processed to avoid causing damage in processes.Generally, the process of seed can be carried out any time between gathering and sowing.Often, the seed separating and having removed cob, shell, stem, epidermis, hair or sarcocarp from plant is used.Such as, the seed gathering, clean and being dried to water capacity less than 15 weight % can be used.Or, it is possible to use dried such as with water-treated and then dry seed.

When processing seed, it is often necessary to note, selecting to be applied to the active agent combinations of the present invention of seed or the amount of the amount of compositions and/or other additives, so that not damaging the germination of seed, or making gained plant not to be caused damage.Especially for the active component being likely to be of phytotoxic effect under some rate of application, it is necessary to be noted that.

The active agent combinations of the present invention can directly be used, and does not namely include any other component and is not diluted.Generally preferable compositions is applied to seed with suitable dosage form.Suitable preparation and method for seed treatment are known for those skilled in the art and have description in such as documents below: US4,272,417, US4,245,432, US4,808,430, US5,876,739, US2003/0176428A1, WO2002/080675, WO2002/028186.

The Seed dressing formulations of routine can will can be converted into, for instance solution, Emulsion, suspending agent, powder, foam, serosity or other seed coating compositions, and ULV preparation according to the active component that the present invention uses.

These preparations are prepared in known manner by being mixed with conventional additives by active component, described conventional additives such as, conventional fillers and solvent or diluent, coloring agent, wetting agent, dispersant, emulsifying agent, defoamer, preservative, secondary thickening agent, binding agent, gibberellins and water.

May be present in can be all coloring agent being usually used in this purpose according to the useful coloring agent in the Seed dressing formulations of present invention use.The pigment being slightly soluble in water can be used, it is possible to use be dissolved in the dyestuff of water.Example includes known name and is called the coloring agent of rhodamine B (RhodamineB), C.I. paratonere 12 (C.I.PigmentRed112) and C.I. solvent red 1 (C.I.SolventRed1).

May be present in can be promote moistening and be conventionally used for all substances in agrochemical active ingredient preparation according to the useful wetting agent in the Seed dressing formulations that the present invention uses.Alkylnaphthalene sulfonate is preferably used, such as diisopropyl naphthalene sulfonate or diisobutyl naphthalene.

May be present in can be conventionally used for all non-ionic, anion in agrochemical active ingredient preparation or cationic dispersant according to the useful dispersant in the Seed dressing formulations that the present invention uses and/or emulsifying agent.Nonionic or anionic dispersing agents or the mixture of nonionic or anionic dispersing agents are preferably used.Suitable non-ionic dispersing agent is particularly including ethylene oxide/propylene oxide block polymer, alkyl phenol polyethylene glycol ethers and triphenyl vinyl phenol polyglycol ether, and its phosphorylation or sulfated derivative.Suitable anionic dispersing agents is in particular lignosulphonates, polyacrylate and arylsulphonate/formaldehyde condensation products.

May be present in can be all foam inhibition material being generally used in agrochemical active ingredient preparation according to the defoamer in the Seed dressing formulations of present invention use.Silicone antifoams agent and magnesium stearate are preferably used.

May be present in can be all substances for this purpose in agrochemical composition according to the preservative in the Seed dressing formulations that the present invention uses.Example includes Dichlorophenol and benzyl alcohol hemiformal.

May be present in can be all substances for this purpose in agrochemical composition according to the secondary thickening agent in the Seed dressing formulations that the present invention uses.Preferred example includes cellulose derivative, acrylic acid derivative, xanthan gum, modified clay and silicon dioxide in small, broken bits.

May be present in can be can be used for dressing seed all traditional binders of product according to the binding agent in the Seed dressing formulations that the present invention uses.Preferred example includes polyvinylpyrrolidone, polyvinyl acetate, polyvinyl alcohol and methylcellulose.

May be present in and can be preferably gibberellins A1, A3 (=gibberellin), A4 and A7 according to the gibberellins in the Seed dressing formulations that the present invention uses；Particularly preferably use gibberellin.

Can directly use according to the Seed dressing formulations that the present invention uses or use after water in advance dilution, for processing the different seeds of wide scope, including the seed of transgenic plant.In this case, with the matter interaction passing through expression formation, it is possible to extra cooperative effect occurs.

For adopt can according to the Seed dressing formulations that the present invention uses or by described Seed dressing formulations by adding water and for seed treatment that the preparation prepared carries out, all mixed cells being conventionally used for dressing seed all can be used.Specifically, carrying out following procedure: be placed in blender by seed in seed dressing operation, add specific the desired amount of Seed dressing formulations (diluted with himself form or water in advance), mixing all substances are until preparation is distributed evenly on seed.If suitable, it is dried step subsequently.

The active agent combinations of the present invention or compositions can for the invasion and attack from undesired microorganism (such as fungus and insecticide) of the safeguard industries material and destructions in material protection.

Additionally, the active agent combinations of the present invention can individually or be incorporated as antifouling composition with other active component.

In the context of the present invention, industrial materials are understood to refer to be produced the nonliving material for industry.Such as, it is intended that by the active agent combinations of the present invention or compositions protect against microorganism change or the industrial materials that damage can be binding agent, sizing material, paper, wallpaper and paper, yarn fabric, carpet, leather, timber, fiber and tissue, coating and plastic, cooling lubricant and other can by the material of microbiological attack or damage.Also the part of workshop and the building that can be damaged by the breeding of microorganism can be mentioned in the material ranges being intended to protection, for instance cooling water circulation, cooling and heating system and heating ventilation and air-conditioning unit.Industrial materials in the scope of the invention preferably include binding agent, sizing material, paper and plate, leather, timber, coating, cooling lubricant and heat transfer liquids, more preferably timber.

The active agent combinations of the present invention or compositions can prevent adverse effect, such as rotten, rottenization, variable color, fade or form mycete.

When processing timber, the active agent combinations of the present invention can be additionally used in opposing and is prone to be grown in the fungal disease of Wood surface or inside.Term " timber (timber) " means all types of timber kind and all types of processed timber that can be used for building, for instance solid wood, high density wood, laminated timber and plywood.The method for processing timber of the present invention essentially consists in the compositions of one or more compounds with the present invention or the present invention and contacts；This includes such as directly using, spray, impregnate, inject or method that any other is suitable.

Additionally, the active agent combinations of the present invention can be used for protecting the object, particularly hull, sieve, net, building, moorings and signaling system that contact with saline or brackish water in case producing stained.

The method for preventing and treating undesired fungus of the present invention can be additionally used in protection and stores article.Storing article and be understood to refer to the natural materials of plant or animal origin or the converted products of its natural origin, it needs digital preservation.The storage article of plant origin; such as, plant or plant parts, such as stem, leaf, tuber, seed, fruit, corn; can after freshly harvested protection or processed after protection, described in be processed as (in advance) and dry, moisten, pulverize, grind, suppress or toast.Store article and also include timber, including unprocessed, for instance building timber, electric pole and fence, or to make the form of product, such as furniture.The storage article of animal origin are, for instance skin, leather, fur and hair.The active agent combinations of the present invention or compositions can prevent adverse effect, such as rotten, rottenization, variable color, fade or form mycete.

The microorganism that can degrade or change industrial materials includes, for instance antibacterial, fungus, yeast, algae and mucus biological (slimeorganism).The active agent combinations of the present invention or compositions are preferred for opposing fungus, particularly mycete, make sapstain and destroy timber fungus (Ascomycetes (Ascomycetes), Basidiomycetes (Basidiomycetes), deuteromycetes (Deuteromycetes) and Zygomycetes (Zygomycetes)), and opposing mucus biology and algae.Example includes the microorganism with subordinate: Alternaria (Alternaria), for instance very thin rod method (Alternariatenuis)；Aspergillus (Aspergillus), for instance aspergillus niger (Aspergillusniger)；Chaetomium (Chaetomium), for instance chaetomium globosum (Chaetomiumglobosum)；Cellar fungus belongs to (Coniophora), for instance Coniophora puteana (Coniophorapuetana)；Lentinus (Lentinus), for instance Lentinus tigrinus bacterium (Lentinustigrinus)；Penicillium (Penicillium), for instance Penicillum glaucum (Penicilliumglaucum)；Polyporus (Polyporus), for instance variegated polypor (Polyporusversicolor)；Aureobasidium (Aureobasidium), for instance Aureobasidium pullulans (Aureobasidiumpullulans)；Core stem point belongs to (Sclerophoma), for instance Sclerophomapityophila；Trichoderma (Trichoderma), for instance Trichoderma viride (Trichodermaviride)；nullLong beak shelly belongs to kind of (Ophiostomaspp.)、Long beak shell belongs to kind of (Ceratocystisspp.)、Humicola kind (Humicolaspp.)、Peter's shell belongs to kind of (Petriellaspp.)、Pieces is mould belongs to kind of (Trichurusspp.)、Coriolous Dersicolor (Fr.) Quel belongs to kind of (Coriolusspp.)、Gill fungus belongs to kind of (Gloeophyllumspp.)、Pleurotus kind (Pleurotusspp.)、Poria belongs to kind of (Poriaspp.)、Dragon cyprid belongs to kind of (Serpulaspp.) and a Tyromyces kind (Tyromycesspp.)、Cladosporium kind (Cladosporiumspp.)、Paecilomyces kind (Paecilomycesspp.)、Mucor kind (Mucorspp.)；Escherichia (Escherichia), for instance escherichia coli (Escherichiacoli)；False unicellular Pseudomonas (Pseudomonas), for instance Pseudomonas aeruginosa (Pseudomonasaeruginosa)；Staphylococcus (Staphylococcus), for instance staphylococcus aureus (Staphylococcusaureus)；Candida (Candidaspp.) and Saccharomyces kind (Saccharomycesspp.), for instance saccharomyces cerevisiae (Saccharomycescerevisae).

Additionally, the active agent combinations of the present invention or compositions also have extraordinary Antifungal action.nullThey have the Antifungal action spectrum of non-constant width，Particularly to dermatophytes and yeast、Candida (Candida) (is such as planted by mycete and diphasic fungi，Such as Candida albicans (C.albicans)、Candida glabrata (C.glabrata)) and acrothesium floccosum (Epidermophytonfloccosum)、Aspergillus (Aspergillus) plants (such as aspergillus niger (A.niger) and Aspergillus fumigatus (A.fumigatus))、Trichophyton (Trichophyton) plants (such as Trichophyton mentagrophytes (T.mentagrophytes))、Microsporon (Microsporon) plants (such as Sabouraudites lanosus (M.canis) and microsporon audouini (M.audouinii)).These cited funguses are not limiting as overlayable mycete spectrum, and merely to explanation.

Therefore, the active agent combinations of the present invention or compositions can be used in medical treatment and non-medical applications.

As having already mentioned above, all of plant and plant parts can be processed according to the present invention.In a preferred embodiment, process wild plant species and plant cultivars, or process those plants and position thereof of being obtained by standard biologic breeding method (such as cross breeding or Protoplast fusion).In another preferred embodiment of the present, the transgenic plant and plant cultivars (biology of genetic modification) and plant parts thereof that are obtained by gene engineering method (if suitable, be combined with conventional method) are processed.Term " position " or " position of plant " or " plant parts " are existing hereinbefore to be explained.It is highly preferred that process the plant of commercially available or in use plant cultivars according to the present invention.Plant cultivars is understood to mean that the plant with new capability (" character (trait) "), and it can pass through conventional breeding, mutation or recombinant DNA technology and obtain.They can be cultigen, mutation, biotype or genotype.

The processing method of the present invention can be used for processing the biology (GMO) of genetic modification, for instance plant or seed.Genetically modified plant (or transgenic plant) is the plant that heterologous gene is stably integrated in genome.Statement " heterologous gene " refers mainly to the gene providing outside plant or assembling, and when they are introduced into Matrix attachment region, Chloroplast gene or mitochondrial genome, by expressing protein interested or polypeptide, or it is present in other genes (utilizing such as antisense technology, co-suppression technology, RNA interference-RNAi technology or microRNA miRNA technology) in plant by downward or silence, and give these transgenic plants agronomy attribute that is new or that improve or other characteristic.It is arranged in the heterologous gene of genome also referred to as transgenic.The transgenic defined by its particular location in Plant Genome is referred to as transformation plant or transgenic line (event).

According to plant species or plant cultivars, its position and growth conditions (soil, weather, trophophase, nutrition), the process of the present invention can also result in super adding and (" working in coordination with ") effect.Such as, desirable the following effect exceeding actual desired: reducing can according to the rate of application of the reactive compound that the present invention uses and compositions and/or widen its activity profile and/or improve its activity, improve plant growing, improve the toleration to high temperature or low temperature, improve arid or the toleration to water or soil salt content, improve performance of blooming, make to gather and be easier to, hasting of maturity, yield of gathering is higher, fruit is bigger, plant is higher, leaf color is greener, bloom earlier, the quality of harvested products and/or its nutritive value are higher, in fruit, the concentration of sugar is higher, storage stability and/or the processability of harvested products are better.

The active agent combinations of the present invention also has invigoration effect in plant.Therefore, they are also applied for adjusting vegeto-animal defense system to resist undesired microorganism.This is likely to, if properly, is one of the active agent combinations of present invention reason of activity with enhancing.In this article, plant strengthening (induction of resistance) material is understood to mean that and can stimulate the material of plant defense system or the combination of material in such a way, namely, when inoculating undesired microorganism subsequently, these microorganisms are shown resistance significantly by treated plant.In this case, undesired microorganism can be understood as and refers to plant pathogenic fungi, antibacterial and virus.Therefore, the infringement that can be used for protecting in the plant regular period after treatment by the material of the present invention from above-mentioned pathogen.After adopting reactive compound to process plant, it is achieved the time of protective effect typically lasts for 1-10 days, it is preferable that 1-7 days.

The plant and the plant cultivars that preferably process according to the present invention include all plants with hereditary material, and described hereditary material gives particularly advantageous, the useful performance of these plants (be no matter by breeding and/or obtained by biological technique method).

Also, it is preferred that one or more biotic factors are had resistance by plant and plant cultivars according to present invention process, namely described plants against animal and microbial pests (as to nematicide, insecticide, demodicid mite class, phytopathogenic fungi, antibacterial, virus and/or viroid) have better defense.

The example of nematicide or insect-resistant plants is, for instance those mentioning in WO2012/045798A1 and WO2012/089757A1.

Can be also that one or more abiotic stress factors are had the plant of resistance according to the plant of present invention process and plant cultivars.Abiotic stress conditions can include, for instance arid, low temperature exposure, beat exposure, osmotic stress, waterlogging, the soil salt content of increase, the mineral exposure of enhancing, ozone exposure, high light expose, limited nitrogen nutrition element availability, limited phosphorus nutrition element availability, keep away shade.

Also can according to the present invention process plant and plant cultivars be with strengthen Yield Characters be feature those plants.The yield of the raising of described plant can be caused by following factor: such as, the plant physiology function improved, g and D, the nitrogen such as water-use efficiency, water holding efficiency, improvement utilizes, the carbon assimilation that strengthens, the photosynthesis of improvement, the germination percentage of raising and acceleration maturation.The impact of the plant architecture (plantarchitecture) that yield also can be improved (coerce with under non-stress condition), include but not limited to: bloom ahead of time, to controls of blooming of hybrid seeds, rice shoot vigor, plant size, internode number and interval, root growth, seed size, fruit size, pod size, pod number or spike number, each pod or the number seeds of fringe, seed quality, the seed plumpness of raising, the seed dispersal of minimizing, minimizing pod ftracture and lodging resistance.Other yield characteristics include seed composition, such as carbohydrate content, protein content, oil content and composition, nutritive value, the minimizing of anti-nutrient compounds, the processability of improvement and better bin stability.

The plant that can process according to the present invention be the hybrid plant having given expression to hybrid vigor or hybrid vigor feature, and it is more healthy and to the better resistance of biotic and abiotic stress that described hybrid vigor or hybrid vigor typically result in higher yield, higher vigor, plant.This kind of plant can be educated parent line (male parent) hybridization usually by by male with another kind of selfing for one selfing male sterility parent line (female parent) and prepare.Hybrid seed is generally gathered from male sterile plants and is sold to grower.Male sterile plants can be prepared by emasculation (namely mechanically removing male reproductive organ or male flower) sometimes (such as in Semen Maydis), but, more generally, male sterility is to be caused by the genetic determinant in Plant Genome.In this case, particularly when seed is until the required product gathered from hybrid plant, it is ensured that the recovery completely of the male fertility in hybrid plant is usually useful.This can by assuring that male parent has suitable restoring gene and completes, and this gene can recover the male fertility of the hybrid plant containing the genetic determinant being responsible for male sterility.It is responsible for male sterile genetic determinant to can be located in Cytoplasm.The example of cytoplasmic male sterility (CMS) crosses (WO92/05251, WO95/09910, WO98/27806, WO2005/002324, WO2006/021972 and US6 described in such as planting in Btassica (Brassica), 229,072).But, it is responsible for male sterile genetic determinant and may be alternatively located in nuclear genome.Male sterile plants obtains also by Plant Biotechnology method such as genetic engineering.A kind of particularly useful method obtaining male sterile plants is recorded in WO89/10396, wherein, for instance, selective expression's ribonuclease such as barnase in the tapetal cell in stamen.Fertilizability (such as WO91/02069) can be recovered subsequently by expression ribonuclease inhibitor such as barnase inhibitive factor in tapetal cell.

Can be herbicide tolerant plants according to the plant of present invention process or plant cultivars (being obtained by Plant Biotechnology method such as genetic engineering), i.e. the plant to one or more given herbicide tolerant.These plants can by genetic transformation or by selecting the plant of the sudden change containing the described herbicide tolerant of imparting to obtain.

Herbicide resistant plants tolerates plant for such as glyphosate (glyphosate), the plant namely herbicide glyphosate or its salt tolerated.Plant can be made glyphosate tolerance by distinct methods, for instance those methods mentioned in WO2012/045798A1 and WO2012/089757A1.

Other plants that herbicide has resistance are, the such as plant to the herbicide tolerant suppressing glutamine synthetase, described herbicide such as bialaphos (bialaphos), grass fourth phosphine (phosphinothricin) or glufosinate-ammonium (glufosinate).The method that these plants can pass through to mention in WO2012/045798A1 and WO2012/089757A1 obtains.

Other herbicide-tolerant plants are also the plant to the herbicide tolerant suppressing hydroxyphenylpyruvic acid dioxygenase (HPPD).HPPD is the enzyme that catalysis p _ Hydroxyphenyl pyruvic acid salt (HPP) is converted into the reaction of 2,5-dihydroxy-.alpha.-toluic acid..Tolerate the gene of the naturally occurring anti-HPPD enzyme of plant available code of HPPD inhibitor or obtain with the gene transformation of encoding mutant or chimeric HPPD enzyme, for instance mentioning in WO2012/045798A1 and WO2012/089757A1.

Other herbicide-tolerant plants are the plants to the tolerance of acetolactate synthestase (ALS) inhibitor.Known ALS inhibitor includes, for instance, sulfonylureas, imidazolone, triazolo pyrimidine, pyrimidine oxygen (sulfur) yl benzoic acid ester and/or sulfonyl amino carbonyl triazolinone herbicide.Different sudden changes in known ALS enzyme (also referred to as acetohydroxy acid synthetase, AHAS) give the toleration (the corresponding reference referring to mentioning in WO2012/045798A1 and WO2012/089757A1) to different herbicides and herbicide group.

Can be also the transgenic plant that insecticide is had resistance according to the plant of present invention process or plant cultivars (being obtained by Plant Biotechnology method such as genetic engineering), namely the invasion and attack of some targeted insect are had the plant of resistance.This kind of plant can by genetic transformation or by selecting the plant containing the sudden change giving described insect-resistant to obtain.

" insect resistant transgenic plant " used herein is particularly to the insect resistant transgenic plant mentioned in WO2012/045798A1 and WO2012/089757A1.

Also according to the plant that the present invention processes or plant cultivars (being obtained such as genetic engineering by Plant Biotechnology method), abiotic stress can be had toleration.This kind of plant can by genetic transformation or by selecting the plant of the sudden change containing the described stress resistance of imparting to obtain.Useful especially stress tolerant plants is those that mention in WO2012/045798A1 and WO2012/089757A1.

The change of the quantity of harvested products, quality and/or bin stability also can be demonstrated according to the plant that the present invention processes or plant cultivars (being obtained by Plant Biotechnology method such as genetic engineering), and/or the change of the special component characteristic of harvested products, those transgenic plants that described plant or plant cultivars are such as mentioned in WO2012/045798A1 and WO2012/089757A1.

Can be also the plant of the oily distribution character with change according to the plant that the present invention processes or plant cultivars (being obtained such as genetic engineering by Plant Biotechnology method), for instance Brassica campestris L and relevant Brassica plants.This kind of plant can by genetic transformation or by selecting the plant of sudden change containing the oily distribution character giving described change to obtain, including those oilseed rape plants mentioned in WO2012/045798A1 and WO2012/089757A1.

Can be the plant containing transformation plant or the combination containing transformation plant according to the useful especially transgenic plant that the present invention processes, these plants are to affix one's name to (APHIS) in the U.S. to the animals and plants sanitary inspection of United States Department of Agriculture (USDA) (USDA) to apply to become the object of non-control state, and no matter described application has gone through or still pending.

Can be containing transformation plant or the plant of combination containing transformation plant according to the useful especially transgenic plant that the present invention processes, be those that mention in WO2012/045798A1 and WO2012/089757A1.

In preferred embodiments, the present invention relates to, several or all of method for realizing in following effect:

-preventing and treating phytopathogenic fungi in plant or on plant,

-in crop protection, prevent and treat phytopathogenic fungi,

-improve the vigor of plant and/or improve development of plants (especially improving plant growing, it is preferable that increase plant growth rate),

-improve pigment content and/or strengthen photosynthetic activity (preferably increasing chlorophyll content, thus preferably obtaining greener leaf (" greening ") and/or bigger leaf (particularly bigger blade)),

-increase Biomass, such as higher fresh weight (FW) and/or dry weight (DW), preferably increase plant weight (grain weight (every) of especially higher fruit weight (each fruit) or higher and/or total grain yield of higher total fruit or higher) and/or increase plant height

-have additional nutrients cellulose content, especially increase micronutrient content, especially increase macronutrient content (especially the row of N, P and/or K is bright), increase protein content (especially the increase of water soluble protein), increase vitamin content (especially vitamin A, B₁、B₂, C and/or E content) and/or increase essential amino acids amount,

Described method is characterised by, the active agent combinations of the present invention or compositions are applied to seed, plant, plant parts (preferably fruit and/or leaf), or in the soil of plant growing or expectation plant growing.

In the context of the present invention, especially, viewed effect is with the active agent combinations of the present invention (as defined above, preferably in defined in one of preferred embodiment) or the compositions (as defined above, it is preferable that defined in one of preferred embodiment) of the present invention leaf at plant or its position is processed in observe.

When using active agent combinations or the compositions of the present invention, rate of application can change in relatively wide scope according to using type.

In a preferred method of the invention, the compositions of the active agent combinations of the present invention or the present invention is applied on seed or leaf, wherein in this is used, the amount of the active agent combinations on leaf (is preferably 500 to 2500g/ha in the scope of 400 to 3000g/ha, it is more preferably 600 to 2000g/ha, and it is even more preferably 700 to 1700g/ha), and in the process of seed in the scope of the every 100kg seed of 2-200g, it is preferably the every 100kg seed of 5-150g, more preferably the every 100kg seed of 10-100g.

Can be used for the compositions of the active agent combinations of the present invention or the present invention protecting in plant a period of time after treatment from mentioned pathogen invasion.After adopting described active component to process plant, it is provided that the time of protection generally continues 1 to 28 day, it is preferred to 1 to 14 day, more preferably 1 to 10 day, it is most preferred that 1 to 7 day；Or up to 200 days after seed treatment.

Listed plant can adopt the active agent combinations of the present invention or the compositions of the present invention particularly advantageously to process according to the present invention.The active agent combinations of the present invention is active, and this activity has exceeded the simple addition of each composition activity.The activity of the improvement of the active agent combinations of the present invention is apparent from from following instance.

When the activity of active agent combinations exceedes the reactive compound active summation when individually using, then there is cooperative effect.

The expection activity of the given combination of two kinds of reactive compounds can be calculated as below (with reference to Colby, S.R., " CalculatingSynergisticandantagonisticResponsesofHerbicid eCombinations ", Weeds1967,15,20-22):

If

X is reactive compound A effect when using with the rate of application of mppm (or g/ha),

Y is reactive compound B effect when using with the rate of application of nppm (or g/ha),

E is reactive compound A and B effect when using with the rate of application of m and nppm (or g/ha) respectively,

Then

The degree of effect represents with %.0% means to be equivalent to effect of matched group.

If the activity observed exceedes value of calculation, then the activity of described combination is surpass to add sum, namely there is cooperative effect.In this case, the effect actually observed is necessarily greater than the expection effect (E) calculated by above-mentioned formula.

On the other hand, the present invention relates to the purposes of the compositions of the active agent combinations of the present invention or the present invention, be used for:

-preventing and treating phytopathogenic fungi in plant or on plant,

-in crop protection, prevent and treat plant pathogenic fungi,

-improve the vigor of plant and/or improve development of plants (especially improving plant growing, it is preferable that increase plant growth rate),

-improve pigment content and/or strengthen photosynthetic activity (preferably increasing chlorophyll content, thus preferably obtaining greener leaf (" greening ") and/or bigger leaf (particularly bigger blade)),

-increase Biomass, such as higher fresh weight (FW) and/or dry weight (DW), preferably increase plant weight (grain weight (every) of especially higher fruit weight (each fruit) or higher and/or total grain yield of higher total fruit or higher) and/or increase plant height

-have additional nutrients cellulose content, especially increase micronutrient content, especially increase macronutrient content (especially the content of N, P and/or K), increase protein content (especially the increase of water soluble protein), increase vitamin content (especially vitamin A, B₁、B₂, C and/or E content) and/or increase essential amino acids amount.

On the other hand, the present invention relates to Propineb for increasing or improve the picked-up in plant of micronutrient (be preferably selected from Mn, B, Zn, Cu, Fe, Mo, Se, Al, Co and Ni a kind of, two kinds, three kinds or more kind micronutrient) and/or macronutrient (one being preferably selected from N, P and K, two kinds or all macronutrients) or by the purposes of the picked-up of plant.

On the other hand, the present invention relates to the compositions of the active agent combinations of the present invention or the present invention for the purposes that the leaf at plant or its position processes, wherein said plant can be transgenic plant.

On the other hand, the present invention relates to the compositions of the active agent combinations of the present invention or the present invention for processing the purposes of seed, the seed of transgenic plant and transgenic plant.

Another aspect, the present invention relates to the seed adopting the active agent combinations of the present invention or the compositions-treated of the present invention to cross.

On the other hand, the present invention relates to test kit, comprising:

(A) Propineb,

With composition (B), it includes

(B1) one or more contain the salt of boron (B), and

(B2) one or more contain the salt of manganese (Mn),

Optionally also include for applying composition (A) and (B) operating instruction with the compositions of the active agent combinations or the present invention that obtain the present invention in some way.

Embodiment:

The combination of Propineb and Mn and B is assessed for the impact of growth characteristics, and have evaluated physiological change.Additionally, have evaluated the nutrition-physiological change caused by described process and disease is prevented, the impact of yield and crop product quality.

In experiment below, Propineb uses with the form of the wettable powder of the Propineb containing 70 weight % content, is called Antracol70WP.Antracol70WP is commercially available from Bayer Cropscience.

Test the field interior-excess at India Coimbatore district TamilNadu to execute.

Crop: Fructus Lycopersici esculenti

Sample area: 50m²

Repeat: 3

Spacing: 45 × 30cm

Process: 8 groups

Spray number: spray for 2 times: 35DAP (natural law after plantation) and 45DAP

Process details:

Adopt following processing mode in an experiment:

T1: matched group

T2:Antracol70WP (1250g/ha)

T3: manganese (0.2%)

T4:Antracol70WP (1250g/ha)+manganese (0.2%)

T5: boron (0.2%)

T6:Antracol70WP (1250g/ha)+boron (0.2%)

T7: manganese (0.2%)+boron (0.2%)

T8:Antracol70WP (1250g/ha)+manganese (0.2%)+boron (0.2%)

Foliage spray (pH:7.3) is carried out with high-quality water

Second time spray after the 20th day, carry out in plant height, root length, the number of sheets and leaf area Morphologic observation and use SPAD measuring device measure leaf chlorophyll index.Additionally, also record yield and its composition when results.Additionally, also record disease score (the early and late droop of Fructus Lycopersici esculenti) and nutrient for plants situation.The result of the various parameters of different disposal provides in each table.

ANOVA bag (AGRES version 7.01) is used to carry out data analysis in the complete block design of randomization the data collected.

The field layout used

Row I

T3

T6

T4

T7

T2

T8

T1

T5

Row II

T6

T8

T5

T3

T1

T4

T2

T7

Row III

T7

T2

T8

T4

T5

T3

T1

T6

Table 1: the initial soil parameter (before process) used in an experiment

Table 2: Propineb, manganese and the boron impact on tomato growth aspect

Growth parameter(s)

About growth parameter(s), described plant height is not reaching to significance,statistical (table 2).The foliage spray of Antracol70WP and Mn and B demonstrates higher plant (81.20cm), next to that Antracol70WP+Mn (T4:79.40cm) and Antracol70WP (T2:79.00cm).

By contrast, the foliage spray being used alone manganese (T3) observed less plant.About root length, have recorded the significantly excellent root length of 27.90cm in accepting the Antracol70WP+Mn T4 processed, this process T4 is suitable with process T8 (Antracol70WP+Mn+B).The control treatment do not sprayed have recorded the minimum of 24.55cm.

Table 3: Propineb, manganese and the boron impact on tomato leaf

Blade characteristic

All blade characteristics have reached significance,statistical (table 3).About the number of sheets, Antracol70WP+Mn+B foliage spray demonstrates the higher number of sheets (36), and this is significantly better than other and processes.About leaf area and leaf area index (LAI), through the Antracol70WP+Mn+B plant record processed corresponding to every strain 673.50cm of LAI (T8:0.50)²Higher leaf area, next to that Antracol70WP (every strain 624.60cm²With 0.46).The process (T5) being used alone boron and carrying out foliage spray is found that every strain 530.39cm²Less leaf area and LAI (0.39).

Table 4: Propineb, manganese and the boron impact on tomato biological chemical parameters

Biochemical parameter

About biochemical parameter, all parameters have reached significance,statistical (table 4).Significantly higher chlorophyll desired value (47.7) is have recorded in processing Antracol70WP+Mn+B, next to that Antracol70WP+ manganese (45.7), Antracol70WP+ boron (45.6) and Antracol70WP (45.5).About soluble protein, go out 11.3mgg through the Antracol70WP+Mn+B plant performance sprayed^-1Higher content of soluble protein, next to that Antracol70WP+B (10.5mgg^-1) and Antracol70WP+Mn (10.2mgg^-1).About not aoxidize the IAA oxidase active that plant growing cellulose content represents, matched group have recorded 15.85 μ gg^-1h^-1Higher do not aoxidize plant growing cellulose content, this is close to individually accepting Antracol70WP T2 (the 15.73 μ gg processed^-1h^-1).The process using Mn demonstrates higher IAA oxidase active (low does not aoxidize plant growing cellulose content), wherein Mn individually shows higher vigor (12.32), next to that Mn+B (T7) and Antracol+Mn+B (T8).

Table 5: Propineb, manganese and the boron impact on tomato plants nutrient content

About nutrient status, all of nutrient has reached significance,statistical (table 5).About nitrogen content, T8 (Antracol70WP+Mn+B) have recorded higher N percentage ratio (1.55%), next to that T6 (Antracol+B:1.42%) and T7 (manganese+boron: 1.40%).In processing T8 (Antracol70WP+Mn+B), have recorded the higher P content percentage ratio of 0.44, be only second to T6 (Antracol70WP+B:0.41), T6 and T7 (Mn+B:0.40) suitable.About K, higher K percentage ratio is again have recorded in processing T8 (Antracol70WP+Mn+B:1.28), next to that it is suitable to process T4 (Antracol70WP+Mn:1.18), T4 and T6 (Antracol70WP+B:1.17) and T2 (Antracol70WP:1.14).

About Fe content, T8 (Antracol70WP+Mn+B) have recorded higher Mn percentage ratio (0.033%), next to that T7 (Mn+B:0.028%) and T4 (Antracol70WP+Mn:0.025%).About the content of boron (B), T8 (Antracol70WP+Mn+B) have recorded higher B percentage ratio, next to that T7 (Mn+B:0.044%) and T6 (Antracol70WP+B:0.040%).Overall performance aspect, have recorded considerably higher N (1.55%), P (0.44%), K (1.28%), Mn (0.033%) and B (0.058%) content processing in Antracol70WP+Mn+B.The control treatment (T1) do not sprayed have recorded relatively low N (1.20%), P (0.32%), K (0.93%), Mn (0.018%) and B (0.028%) content.

Table 6: Propineb, manganese and boron are to Fructus Lycopersici esculenti crop growth rate (CGR) (gm^-2d^-1) impact

Crop growth rate (CGR) (gm^-2d^-1)

Falling into a trap calculation crop growth rate: 60-90 days and 90-120 days (DAP) after plantation in following two stages, crop growth rate has all reached significance,statistical (table 6) in two stages of plant growth.The plant processed through the foliage spray of Antracol70WP+Mn+B (T8) have recorded 27.35 and 14.84gm respectively during 60-90 and 90-120DAP^-2d^-1Higher growth rate.T8 and process Antracol70WP+B (T6:26.69gm^-2d^-1) and Antracol70WP+ manganese (T4:26.69gm^-2d^-1) crop growth rate during 60-90DAP is suitable.Untreated check plant have recorded 24.29gm at 60-90DAP^-2d^-1The relatively low speed of growth.

Table 7: Propineb, manganese and the boron impact on tomato yield parameter and fruit yield

Yield parameters

About yield parameters, beyond the fruit diameter that dewaters, all of yield parameters has all reached significance,statistical (table 7).About Mean Fruit Weight, reach the higher average weight of 27.85g through Antracol70WP+Mn+B (T8) fruit processed, this is with process Antracol70WP+B (T6:27.46), Antracol70WP+Mn (T4:27.32) and Antracol70WP (T2:27.15) quite.But, heavily increase by 6.6% (T8) relative to matched group (T1:26.13g) fruit do not sprayed.About volume of fruits, through the Antracol70WP+Mn+B fruit sprayed, the volume of fruit bigger (28.96cc), it increases 8.9% relative to matched group (26.59cc).Similar trend is the same with the situation of fruit weight.Fruit diameter is not reaching to significance,statistical.Have recorded higher fruit diameter and in matched group, obtain relatively low fruit diameter (5.36) in processing T8.

About fruit number, have recorded the higher amount of every 29 fruits of strain plant in Antracol70WP+Mn+B processes, it is suitable with the process of Antracol70WP+B (T6:28).The matched group (T1:23) not sprayed have recorded less fruit number.Compared with T3 and T1, T8 have recorded the fruit number of the increase of 26.1%.About fruit yield, having gathered in the crops the higher fruit yield of every piece of sample ground 251kg through Antracol70WP+Mn+B (T8) sample sprayed, it is suitable with the fruit yield processing T6 (Antracol70WP+B) the every piece of sample ground 245kg gathered in the crops.The adjoining tree (T1) do not sprayed have recorded relatively low fruit yield (every piece of sample ground 224kg).Calculate the percentage ratio of yield increase and compare these process.Compared with the matched group not sprayed, Antracol70WP+Mn+B have recorded the higher percent increments of 12.1%, next to that Antracol70WP+B (8.0) and Antracol70WP+Mn (9.4).Again comparing Antracol process, compared with the matched group not sprayed, Antracol70WP+Mn+B have recorded the fruit yield increment of 8.2%, next to that Antracol70WP+B (5.6) and Antracol70WP+Mn (4.3).

Table 8: Propineb, manganese and the boron impact on tomato disease percentage ratio index

About disease exponential quantity (table 8), in the Antracol sprinkling carrying out foliage spray with Antracol70WP+Mn+B (T8:13.22), Antracol70WP+B (T6:15.33), Antracol70WP+Mn (T4:13.42) and Antracol70WP (T2:15.66) processes, observed less disease percentage ratio index.The comparison Tanaka not sprayed, it was observed that higher disease percentage ratio exponential quantity (87.64).Relative to the matched group not sprayed, Antracol70WP+Mn+B have recorded the higher percentage drop of 84.92%, next to that Antracol70WP+Mn (84.69), Antracol70WP+B (82.51) and Antracol70WP (82.13).

Table 9: Propineb, manganese and the boron impact on Tomato Quality parameter

Q factor

About Q factor, total soluble solids (TSS) content has reached significance,statistical (table 9).About content of lycopene, in processing Mn+B, have recorded 4.75mg100g^-1The higher amount of fruit, next to that be used alone the process (4.68) of B.In the matched group not sprayed, (T1:4.51) have recorded minimum.Considering TSS value, have recorded the higher brix value of 4.3 through Antracol70WP+Mn+B (T8) plant sprayed, it is suitable with T7 (manganese+boron: 4.2), next to that T6 (4.1).Calculate the fruit weight percent loss at room temperature stored after from first day of results to results the 5th day.Matched group and T4 (Antracol70WP+Mn) have recorded higher percentage ratio.The relatively low percentage ratio of 27.2% is have recorded in processing T5 (independent boron), T7 (manganese+boron) and T8 (Antracol70WP+Mn+B).

Conclusion

The foliage spray using the combination of Antracol70WP and Mn and B on tomato plant shows well in final fruit yield in the sickness rate trend of g and D and reduction.

-in Fructus Lycopersici esculenti, illustrate the root length increment of 13.4% than the matched group that do not spray through the foliage spray of Antracol70WP and Mn and B；

-by the foliage spray of Antracol70WP and Mn and B, it was observed that the increment of the 23.6% of chlorophyll index (SPAD value) and the increment of soluble protein (24.2%)；

-compared with the comparison do not sprayed, processed by Antracol70WP and Mn and B, have recorded the improvement of tomato plant nutrient status, N (1.55%), P (0.44%), K (1.28%), Mn (0.033%) and B content (0.058%)；

-processed by Antracol and Mn and B, plant growth speed adds 12.6%, this generates the fruit number increment of 26.1%；

-compared with the comparison do not sprayed, the foliage spray of Antracol70WP and Mn and B have recorded 12.1% fruit yield increment.

Claims (15)

1. active agent combinations, comprising:

(A) Propineb,

With component (B), comprising:

(B1) one or more contain the salt of boron (B), and

(B2) one or more contain the salt of manganese (Mn).

2. active agent combinations according to claim 1, wherein the one of composition (B1), several or all components is selected from the oxide of boron and boric acid class and their salt.

3. the active agent combinations according to claim 1 or 2, wherein the one of composition (B1), several or all components is selected from diboron trioxide (B₂O₃)、H₃BO₃、H₂B₄O₇, (preferred Borax, also referred to as sodium borate, sodium tetraborate or disodium tetraborate, it is preferable that borax anhydrous (Na for boric acid sodium salt₂B₄O₇), borax pentahydrate (Na₂B₄O₇·5H₂Or borax decahydrate (Na O)₂B₄O₇·10H₂O)), boric acid potassium salt (preferred K₂B₄O₇And hydrate, it is preferable that its tetrahydrate K₂B₄O₇·4H₂O), boric acid calcium salt (preferred CaB₄O₇、Ca₃(BO₃)₂And hydrate), boric acid magnesium salt (preferred MgB₄O₇、Mg₃(BO₃)₂And hydrate), potassium borohydride, sodium borohydride, potassium borotartrate and nickel borate (II).

4. active agent combinations as claimed in one of claims 1-3, wherein the one of composition (B2), several or all components is selected from manganese (II) salt, it is preferable that selected from manganese acetate (II), manganese sulfate (II), manganese chloride (II), manganese nitrate (II) and manganese phosphate (II).

5. active agent combinations as claimed in one of claims 1-4, wherein

Composition (B1) includes disodium tetraborate and/or disodium tetraborate hydrate or is made up of disodium tetraborate and/or disodium tetraborate hydrate,

And/or

Composition (B2) includes manganese sulfate (II) and/or manganese chloride (II) or is made up of manganese sulfate (II) and/or manganese chloride (II).

6. active agent combinations as claimed in one of claims 1-5, wherein composition (A) relative to the weight ratio of the boron of composition (B) and the gross weight of manganese in the scope of 1250:1 to 25:1, preferably in the scope of 1000:1 to 50:1, more preferably in the scope of 500:1 to 75:1, and particularly preferably in, in the scope of 350:1 to 100:1, being each based on the gross weight gauge of described active agent combinations.

7. active agent combinations as claimed in one of claims 1-6, wherein the total amount of boron [composition (B1)] relative to the weight ratio of the total amount of magnesium [composition (B2)] in the scope of 3:1 to 1:3, preferably in the scope of 2:1 to 1:2, more preferably in the scope of 3:2 to 2:3, even more preferably in the scope of 4:3 to 3:4, and most preferably at, in the scope of 5:4 to 4:5, being each based on the gross weight gauge of described active agent combinations.

8. active agent combinations as claimed in one of claims 1-7, wherein said conjugate also includes:

-the one in zinc (Zn), copper (Cu), ferrum (Fe), molybdenum (Mo), selenium (Se), aluminum (Al), cobalt (Co) and nickel (Ni), several or all of micronutrient

And/or

-the one in nitrogen (N), phosphorus (P) and potassium (K), several or all of macronutrient.

9. compositions, comprising:

(i) active agent combinations any one of claim 1 to 8,

(ii) water, and

(iii) one or more adjuvants (auxiliary agent), it is preferable that one or more are selected from the adjuvant of organic solvent, surfactant, inorganic carrier, organic carrier and other filleies.

10. compositions according to claim 9, wherein total amount is:

(i) described active agent combinations as claimed in one of claims 1-8, it is in the scope of 0.05-0.5 weight %, it is preferable that in the scope of 0.1-0.3 weight %,

And/or

(ii) water, it is in the scope of 70-99.9 weight %, it is preferable that in the scope of 85-99.8 weight %,

It is each based on the gross weight gauge of described compositions.

11. the method being used for one, several or all items realizing in following effect:

-preventing and treating plant pathogenic fungi in plant or on plant,

-in crop protection, prevent and treat plant pathogenic fungi,

-improve the vigor of plant and/or improve development of plants (especially improving plant growing, it is preferable that increase plant growth rate),

-improve pigment content and/or strengthen photosynthetic activity (preferably increasing chlorophyll content, thus preferably obtaining greener leaf (" greening ") and/or bigger leaf (particularly bigger blade)),

-increase Biomass, such as higher fresh weight (FW) and/or dry weight (DW), preferably increase plant weight (grain weight (every) of especially higher fruit weight (each fruit) or higher and/or total grain yield of higher total fruit or higher) and/or increase plant height

-have additional nutrients cellulose content, especially increase micronutrient content, especially increase macronutrient content (especially the content of N, P and/or K), increase protein content (especially the increase of water soluble protein), increase vitamin content (especially vitamin A, B₁、B₂, C and/or E content) and/or increase essential amino acids amount,

The method is characterized in that: active agent combinations as claimed in one of claims 1-8 or compositions according to claim 9 or 10 are applied to seed, plant, plant parts (preferably fruit and/or leaf), or in the soil of plant growing or expectation plant growing.

12. method according to claim 11, it is characterized in that: described active agent combinations or described compositions are applied to seed or leaf, wherein in using, the amount of the described active agent combinations on leaf (is preferably 500 to 2500g/ha in the scope of 400 to 3000g/ha, it is more preferably 600 to 2000g/ha, and it is even more preferably 700 to 1700g/ha), and in the process of seed in the scope of the every 100kg seed of 2-200g, it is preferably the every 100kg seed of 5-150g, more preferably the every 100kg seed of 10-100g.

13. active agent combinations any one of claim 1 to 8 or the purposes of compositions according to claim 9 or 10, it is used for:

-preventing and treating plant pathogenic fungi in plant or on plant,

-in crop protection, prevent and treat plant pathogenic fungi,

-improve the vigor of plant and/or improve development of plants (especially improving plant growing, it is preferable that increase plant growth rate),

-improve pigment content and/or strengthen photosynthetic activity (preferably increasing chlorophyll content, thus preferably obtaining greener leaf (" greening ") and/or bigger leaf (particularly bigger blade)),

-increase Biomass, such as higher fresh weight (FW) and/or dry weight (DW), preferably increase plant weight (grain weight (every) of especially higher fruit weight (each fruit) or higher and/or total grain yield of higher total fruit or higher) and/or increase plant height

-have additional nutrients cellulose content, especially increase micronutrient content, especially increase macronutrient content (especially the content of N, P and/or K), increase protein content (especially the increase of water soluble protein), increase vitamin content (especially vitamin A, B₁、B₂, C and/or E content) and/or increase essential amino acids amount.

14. adopt the seed that the compositions-treated of the active agent combinations any one of claim 1 to 8 or claim 9 or 10 is crossed.

15. test kit, it includes

(A) Propineb,

With composition (B), it includes

(B1) one or more contain the salt of boron (B), and

(B2) one or more contain the salt of manganese (Mn),

Optionally also include using in some way composition (A) and (B) to obtain the operating instruction of active agent combinations any one of claim 1 to 8 or compositions according to claim 9 or 10.