CN105759026A - Preparation method and application of simple electrochemiluminescence immunosensor based on compound semiconductor nanomaterial - Google Patents
Preparation method and application of simple electrochemiluminescence immunosensor based on compound semiconductor nanomaterial Download PDFInfo
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- CN105759026A CN105759026A CN201610126877.4A CN201610126877A CN105759026A CN 105759026 A CN105759026 A CN 105759026A CN 201610126877 A CN201610126877 A CN 201610126877A CN 105759026 A CN105759026 A CN 105759026A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57407—Specifically defined cancers
- G01N33/57438—Specifically defined cancers of liver, pancreas or kidney
Abstract
A preparation method and application of simple electrochemiluminescence immunosensor based on a compound semiconductor nanomaterial. The invention relates to the technical field of electrochemiluminescence immunosensor, particularly relates to a preparation method and application of a simple immunosensor using a graphite-like carbon nitride and molybdenum disulfide nanocomposite (g-C3N4 / MoS2) as a luminescent material and a base material. The two semiconductor nanomaterials, g-C3N4 and MoS2, with similar band gaps are compounded to effectively improve the luminous intensity of the sensor and enhance stability. Based on the specific binding of antigen and antibody, the sensor is used to detect alpha-fetoprotein; and according to different degrees of hinder on electron transfer of alpha-fetoprotein with different concentrations, the sensor has different electrochemiluminescence intensities, so as to achieve the hdetection of alpha-fetoprotein.
Description
Technical field
The present invention relates to electrochemiluminescimmunosensor immunosensor technical field, particularly relate to a kind of with class graphitic nitralloy carbon and molybdenum disulfide nano compound (g-C3N4/MoS2) it is the preparation method and application of the simple type immunosensor of luminescent material and base material.By g-C3N4And MoS2Semiconductor nano material that two kinds of band gap are close is compound can be effectively improved the luminous intensity of sensor and strengthen stability.Specific binding based between antigen-antibody, this sensor is used for detecting alpha-fetoprotein, hinders degree different electron transmission according to the alpha-fetoprotein of variable concentrations, then makes sensor electrogenerated chemiluminescence intensity different, it is achieved the detection of alpha-fetoprotein.
Background technology
Alpha-fetoprotein (AFP) is a kind of important tumor markers, when liver cell generation canceration, AFP content in serum can sharply increase, and AFP raises in serum, it may also occur that in teratoma, testis and ovarian neoplasm etc., therefore detect one of the important means that the content of AFP is diagnosing primary liver cancer.So being badly in need of development a kind of simplicity, the method for quick, sensitive detection AFP, it is achieved the early diagnosis of liver cancer.
The method measuring AFP at present has a lot, such as enzyme-linked immunosorbent assay, high performance liquid chromatography, microbial method etc..These methods have higher sensitivity and selectivity, but detection process needs expensive instrumentation, need complicated pre-treatment and complex operation, sample consumption relatively big, are not suitable for quickly detecting.The advantages such as it is simple that electrochemiluminescimmunosensor immunosensor has equipment, easy to operate, highly sensitive, the best, and detection speed is fast.Therefore the present invention is prepared for a kind of based on g-C3N4/MoS2Simple type electrochemiluminescimmunosensor immunosensor, immunological method is combined with electrogenerated chemiluminescence technology, by the Sensitive Detection to AFP of the specific binding realization between antigen-antibody.
The present invention utilizes drop-coating by g-C3N4/MoS2Modify glassy carbon electrode surface, g-C3N4 And MoS2Being all good two-dimensional semiconductor material, specific surface area is big, film forming ability is strong, can fix lot of antibodies as base material.Meanwhile, MoS2And g-C3N4There is close bandgap structure, can effectively avoid g-C3N4Electron hole pair compound, strengthen photoelectric transformation efficiency, improve the strength and stability of electrogenerated chemiluminescence.It is simple that the electrochemiluminescimmunosensor immunosensor built in the present invention has preparation process, low cost, good stability, sensitivity advantages of higher, provides a kind of feasible method for AFP detection.
Summary of the invention
It is an object of the invention to g-C3N4/MoS2For substrate and luminescent material, build a kind of simple and quick, good stability, highly sensitive electrochemiluminescimmunosensor immunosensor, and this electrochemiluminescimmunosensor immunosensor is used for the Sensitive Detection of AFP.
The technical scheme is that
1. one kind based on g-C3N4/MoS2The preparation method of the simple type electrochemiluminescimmunosensor immunosensor of nano composite material:
(1) with 1.0 μm, 0.3 μm, the Al of 0.05 μm2O3Polishing powder is polished the glass-carbon electrode (GCE) of a diameter of 4 mm successively, and ultrasonic cleaning 3 min in ethanol and ultra-pure water respectively, nitrogen dries up, and takes 6 ~ 10 μ L g-C3N4/MoS2Nano composite material dispersant liquid drop is coated onto under electrode surface, room temperature and dries film forming, rinses the g-C not being bonded with removing with PBS (pH 7.4) cushioning liquid3N4/MoS2Obtain g-C3N4/MoS2/GCE；
(2) alpha-fetoprotein antibody (anti-AFP) the standard liquid drop coating of 10 μ L 8 ~ 10 μ g/mL is taken to electrode surface, incubated overnight at 4 DEG C, rinses to obtain anti-AFP/g-C with PBS (pH 7.4) cushioning liquid3N4/MoS2/GCE；
(3) bovine serum albumin(BSA) (BSA) solution drop coating that 10 μ L mass fractions are 1 ~ 3% is taken to electrode surface, 1 h is hatched at 37 DEG C, close the site of non-specific binding, rinse electrode surface with PBS (pH 7.4) cushioning liquid and obtain BSA/anti-AFP/ g-C3N4/MoS2/GCE；
(4) the alpha-fetoprotein antigen standard liquid dripping a series of variable concentrations that 10 μ L concentration are 0.005 ~ 100 ng/mL is used for and antibody specific recognition, 60 min are hatched at 37 DEG C, rinse electrode surface with PBS (pH 7.4) cushioning liquid, prepare a kind of based on g-C3N4/MoS2Simple type electrochemiluminescimmunosensor immunosensor (AFP/BSA/anti-AFP/g-C3N4/MoS2/GCE)。
2.g-C3N4/MoS2The preparation of dispersion liquid:
(1) g-C3N4Nanometer sheet (g-C3N4 NSs) preparation
Weighing 15 g urea in the crucible of semi-closed, with Muffle furnace with 5 DEG C/min temperature programming to 550 DEG C, calcining at constant temperature 2.5 h obtains pale yellow powder g-C3N4；
Weigh the g-C that 100 mg prepare3N4Powder, in conical flask, adds 100 mL ultra-pure waters, ultrasonic 16 h, is centrifuged by gained solution, removes undispersed g-C under 7000 r/s3N4, collect supernatant, steam at 60 DEG C of backspins and obtain milky g-C3N4 NSs dispersion liquid；
(2) g-C3N4/MoS2The preparation of dispersion liquid
Pipette the MoS of 1 mL1mg/mL2Solution joins the g-C of 7 mL3N4 In NSs dispersion liquid, it is stirred overnight, dilutes with 8 mL ultra-pure waters again after centrifuge washing and i.e. obtain g-C3N4/MoS2Dispersion liquid.
The detection of 3.AFP:
(1) AFP/BSA/anti-AFP/g-C3N4/MoS2/ GCE is working electrode, and Ag/AgCl electrode is reference electrode, and platinum electrode is to electrode；Using MPI-B type multi-parameter chemiluminescence analysis test system to test, the high pressure of photomultiplier is set to 800 V, sweep interval is-1.5 ~ 0 V, and sweep speed is 100 mV/s；
(2) contain in the electrolytic cell of PBS (pH 7.4) cushioning liquid of 0.1 mol/L potassium peroxydisulfate at 10 mL, by the electrogenerated chemiluminescence intensity of the electrode of MPI-B type multi-parameter chemiluminescence analysis test system detection 0.005 ~ 100 ng/mL a series of variable concentrations AFP standard liquid, drawing curve；
(3) testing sample solution replace AFP standard liquid detect.
The invention have the benefit that
(1) present invention is with g-C3N4/MoS2Two-dimensional semiconductor nano material does substrate, and specific surface area is big, film forming ability is strong, can sessile antibody in a large number；
(2) present invention is g-C3N4/MoS2Luminescent material drop coating to electrode surface, hinders electron transmission by protein thus changes the mechanism detection AFP of luminous intensity, without enzyme, unmarked, greatly simplify electrode production process；
(3) present invention g-C3N4And MoS2The semi-conducting material that two kinds of band gap are close is combined, and improves photoelectric transformation efficiency and stability, effectively enhances electrogenerated chemiluminescence intensity；
(4) electrochemiluminescimmunosensor immunosensor prepared by the present invention is for the detection of AFP, simple to operate, range of linearity width, and detection limit is low, it is possible to achieve the detection simple, quick, highly sensitive to AFP.The range of linearity is 0.005 ~ 100 ng/mL, and detection is limited to 2.5 pg/mL.
Accompanying drawing illustrates:
Fig. 1 is the electrogenerated chemiluminescence intensity map of variable concentrations AFP；
Fig. 2 is electrogenerated chemiluminescence intensity and the lg of variable concentrations AFPcLinear Fit Chart.
Wherein, being represented the concentration of AFP respectively by the electrogenerated chemiluminescence intensity map of a to k in Fig. 1 is 0,0.005,0.01,0.1,0.25,0.5,1,5,10,50,100 ng/mL.
Detailed description of the invention:
In order to be more fully understood that the present invention, describe technical scheme in detail with instantiation below, but the invention is not limited in this.
1. one kind based on g-C3N4/MoS2The preparation method of the simple type electrochemiluminescimmunosensor immunosensor of nano composite material:
(1) with 1.0 μm, 0.3 μm, the Al of 0.05 μm2O3Polishing powder is polished the glass-carbon electrode (GCE) of a diameter of 4 mm successively, and ultrasonic cleaning 3 min in ethanol and ultra-pure water respectively, nitrogen dries up, and takes 6 ~ 10 μ L g-C3N4/MoS2Nano composite material dispersant liquid drop is coated onto under electrode surface, room temperature and dries film forming, rinses the g-C not being bonded with removing with PBS (pH 7.4) cushioning liquid3N4/MoS2Obtain g-C3N4/MoS2/GCE；
(2) alpha-fetoprotein antibody (anti-AFP) the standard liquid drop coating of 10 μ L 8 ~ 10 μ g/mL is taken to electrode surface, incubated overnight at 4 DEG C, rinses to obtain anti-AFP/g-C with PBS (pH 7.4) cushioning liquid3N4/MoS2/GCE；
(3) bovine serum albumin(BSA) (BSA) solution drop coating that 10 μ L mass fractions are 1 ~ 3% is taken to electrode surface, 1 h is hatched at 37 DEG C, close the site of non-specific binding, rinse electrode surface with PBS (pH 7.4) cushioning liquid and obtain BSA/anti-AFP/ g-C3N4/MoS2/GCE；
(4) the alpha-fetoprotein antigen standard liquid dripping a series of variable concentrations that 10 μ L concentration are 0.005 ~ 100 ng/mL is used for and antibody specific recognition, 60 min are hatched at 37 DEG C, rinse electrode surface with PBS (pH 7.4) cushioning liquid, prepare a kind of based on g-C3N4/MoS2Simple type electrochemiluminescimmunosensor immunosensor (AFP/BSA/anti-AFP/g-C3N4/MoS2/GCE)。
2.g-C3N4/MoS2The preparation of dispersion liquid:
(1) g-C3N4Nanometer sheet (g-C3N4 NSs) preparation
Weighing 15 g urea in the crucible of semi-closed, with Muffle furnace with 5 DEG C/min temperature programming to 550 DEG C, calcining at constant temperature 2.5 h obtains pale yellow powder g-C3N4；
Weigh the g-C that 100 mg prepare3N4Powder, in conical flask, adds 100 mL ultra-pure waters, ultrasonic 16 h, is centrifuged by gained solution, removes undispersed g-C under 7000 r/s3N4, collect supernatant, steam at 60 DEG C of backspins and obtain milky g-C3N4 NSs dispersion liquid；
(2) g-C3N4/MoS2The preparation of dispersion liquid
Pipette the MoS of 1 mL 1mg/mL2Solution joins the g-C of 7 mL3N4 In NSs dispersion liquid, it is stirred overnight, dilutes with 8 mL ultra-pure water water again after centrifuge washing and i.e. obtain g-C3N4/MoS2Dispersion liquid.
The detection of 3.AFP:
(1) AFP/BSA/anti-AFP/g-C3N4/MoS2/ GCE is working electrode, and Ag/AgCl electrode is reference electrode, and platinum electrode is to electrode；Using MPI-B type multi-parameter chemiluminescence analysis test system to test, the high pressure of photomultiplier is set to 800 V, sweep interval is-1.5 ~ 0 V, and sweep speed is 100 mV/s；
(2) contain in the electrolytic cell of PBS (pH 7.4) cushioning liquid of 0.1 mol/L potassium peroxydisulfate at 10 mL, by the electrogenerated chemiluminescence intensity of the electrode of MPI-B type multi-parameter chemiluminescence analysis test system detection 0.005 ~ 100 ng/mL a series of variable concentrations AFP standard liquid, drawing curve；
(3) testing sample solution replace AFP standard liquid detect.
Owing to serum having gas chromatography molecule, therefore in quick, the Sensitive Detection of alpha-fetoprotein, must take into the impact of other materials, the present invention has investigated material bovine serum albumin common in serum, glucose, carcinomebryonic antigen, the vitamin C response condition to modified electrode, result shows that the mensuration of alpha-fetoprotein is disturbed the least by these materials, it is seen that prepared AFP/BSA/anti-AFP/g-C3N4/MoS2/ GCE has higher selectivity to alpha-fetoprotein.This modified electrode has good biocompatibility, high sensitivity and stability, provides new method support for work such as clinical diagnosis, the Preventives monitoring tumour.
Claims (3)
1. one kind based on g-C3N4/MoS2The preparation method of the simple type electrochemiluminescimmunosensor immunosensor of nano composite material, it is characterised in that comprise the following steps:
(1) with 1.0 μm, 0.3 μm, the Al of 0.05 μm2O3Polishing powder is polished the glass-carbon electrode (GCE) of a diameter of 4 mm successively, and ultrasonic cleaning 5 min in ethanol and ultra-pure water respectively, nitrogen dries up, and takes 6 ~ 10 μ L g-C3N4/MoS2Nano composite material dispersant liquid drop is coated onto under electrode surface, room temperature and dries film forming, with PBS (pH
7.4) cushioning liquid rinses the g-C not being bonded with removing3N4/MoS2Obtain g-C3N4/MoS2/GCE；
(2) alpha-fetoprotein antibody (anti-AFP) the standard liquid drop coating of 10 μ L 8 ~ 10 μ g/mL is taken to electrode surface, incubated overnight at 4 DEG C, use PBS
(pH 7.4) cushioning liquid rinses to obtain anti-AFP/g-C3N4/MoS2/GCE；
(3) bovine serum albumin(BSA) (BSA) solution drop coating that 10 μ L mass fractions are 1 ~ 3% is taken to electrode surface, 1 h is hatched at 37 DEG C, close the site of non-specific binding, rinse electrode surface with PBS (pH 7.4) cushioning liquid and obtain BSA/anti-AFP/ g-C3N4/MoS2/GCE；
(4) the alpha-fetoprotein antigen standard liquid dripping a series of variable concentrations that 10 μ L concentration are 0.005 ~ 100 ng/mL is used for and antibody specific recognition, 60 min are hatched at 37 DEG C, rinse electrode surface with PBS (pH 7.4) cushioning liquid, prepare a kind of based on g-C3N4/MoS2Simple type electrochemiluminescimmunosensor immunosensor (AFP/BSA/anti-AFP/g-C3N4/MoS2/GCE)。
One the most according to claim 1 is based on g-C3N4/MoS2The preparation method of simple type electrochemiluminescimmunosensor immunosensor, described g-C3N4/MoS2Dispersion liquid, it is characterised in that making step is as follows:
(1) g-C3N4Nanometer sheet (g-C3N4 NSs) preparation
Weighing 15 g urea in the crucible of semi-closed, with Muffle furnace with 5 DEG C/min temperature programming to 550 DEG C, calcining at constant temperature 2.5 h obtains pale yellow powder g-C3N4；
Weigh the g-C that 100 mg prepare3N4Powder, in conical flask, adds 100 mL ultra-pure waters, ultrasonic 16 h, is centrifuged by gained solution, removes undispersed g-C under 7000 r/s3N4, collect supernatant, steam at 60 DEG C of backspins and obtain milky g-C3N4 NSs dispersion liquid；
(2) g-C3N4/MoS2The preparation of dispersion liquid
Pipette the MoS of 1 mL 1mg/mL2Solution joins the g-C of 7 mL3N4 In NSs dispersion liquid, it is stirred overnight, dilutes with 8 mL ultra-pure water water again after centrifuge washing and i.e. obtain g-C3N4/MoS2Dispersion liquid.
One prepared by preparation method the most according to claim 1 is based on g-C3N4/MoS2Simple type electrochemiluminescimmunosensor immunosensor, it is characterised in that for the detection of AFP, detecting step is as follows:
(1) AFP/BSA/anti-AFP/g-C3N4/MoS2/ GCE is working electrode, and Ag/AgCl electrode is reference electrode, and platinum electrode is to electrode；Using MPI-B type multi-parameter chemiluminescence analysis test system to test, the high pressure of photomultiplier is set to 800 V, sweep interval is-1.5 ~ 0 V, and sweep speed is 100 mV/s；
(2) contain in the electrolytic cell of PBS (pH 7.4) cushioning liquid of 0.1 mol/L potassium peroxydisulfate at 10 mL, by the electrogenerated chemiluminescence intensity of the electrode of MPI-B type multi-parameter chemiluminescence analysis test system detection 0.005 ~ 100 ng/mL a series of variable concentrations AFP standard liquid, drawing curve；
(3) testing sample solution replace AFP standard liquid detect.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106323952A (en) * | 2016-11-01 | 2017-01-11 | 济南大学 | Preparation method and application of electroluminescent immune sensor based on dual-co-reaction reagent amplifying signal |
| CN107247079A (en) * | 2017-07-19 | 2017-10-13 | 济南大学 | A kind of electrochemiluminescdetection detection method of easy glyphosate pesticide |
| CN109324037A (en) * | 2018-10-09 | 2019-02-12 | 济南大学 | The building of alpha-fetoprotein bipolar electrode paper chip Electrochemiluminescsensor sensor |
| CN110687177A (en) * | 2019-11-26 | 2020-01-14 | 济南大学 | Based on g-C3N4Preparation and application of electrochemical luminescence sensor for detecting procalcitonin through energy transfer between CuO and electrochemical luminescence sensor |
| CN115108587A (en) * | 2022-06-24 | 2022-09-27 | 上海交通大学医学院附属仁济医院 | Molybdenum disulfide-doped two-dimensional carbon nitrogen compound matrix and preparation method and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1014162A1 (en) * | 1998-12-15 | 2000-06-28 | Etat Français représenté par le Délégué Général pour l' Armement | Method and photoactive device for broadband limitation of a light flux |
| CN104891997A (en) * | 2015-05-27 | 2015-09-09 | 青岛大学 | Preparation method of graphite phase carbon nitride/molybdenum sulfide composite material |
| CN105044083A (en) * | 2015-08-03 | 2015-11-11 | 济南大学 | Method for preparing electrochemiluminescence immunosensor for alpha fetoprotein based on Au-g-C3N4 nanocomposite and application |
| CN105067690A (en) * | 2015-07-09 | 2015-11-18 | 济南大学 | Preparation method of electrochemical immunosensor used for detection of estradiol and built on basis of MoS2 composite |
| CN105158479A (en) * | 2015-08-03 | 2015-12-16 | 济南大学 | Preparation method of oncofetal antigen electrochemical immunosensor based on AuNPs-PDDA-GR composite material and application thereof |
-
2016
- 2016-03-07 CN CN201610126877.4A patent/CN105759026A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1014162A1 (en) * | 1998-12-15 | 2000-06-28 | Etat Français représenté par le Délégué Général pour l' Armement | Method and photoactive device for broadband limitation of a light flux |
| CN104891997A (en) * | 2015-05-27 | 2015-09-09 | 青岛大学 | Preparation method of graphite phase carbon nitride/molybdenum sulfide composite material |
| CN105067690A (en) * | 2015-07-09 | 2015-11-18 | 济南大学 | Preparation method of electrochemical immunosensor used for detection of estradiol and built on basis of MoS2 composite |
| CN105044083A (en) * | 2015-08-03 | 2015-11-11 | 济南大学 | Method for preparing electrochemiluminescence immunosensor for alpha fetoprotein based on Au-g-C3N4 nanocomposite and application |
| CN105158479A (en) * | 2015-08-03 | 2015-12-16 | 济南大学 | Preparation method of oncofetal antigen electrochemical immunosensor based on AuNPs-PDDA-GR composite material and application thereof |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106323952A (en) * | 2016-11-01 | 2017-01-11 | 济南大学 | Preparation method and application of electroluminescent immune sensor based on dual-co-reaction reagent amplifying signal |
| CN107247079A (en) * | 2017-07-19 | 2017-10-13 | 济南大学 | A kind of electrochemiluminescdetection detection method of easy glyphosate pesticide |
| CN109324037A (en) * | 2018-10-09 | 2019-02-12 | 济南大学 | The building of alpha-fetoprotein bipolar electrode paper chip Electrochemiluminescsensor sensor |
| CN110687177A (en) * | 2019-11-26 | 2020-01-14 | 济南大学 | Based on g-C3N4Preparation and application of electrochemical luminescence sensor for detecting procalcitonin through energy transfer between CuO and electrochemical luminescence sensor |
| CN110687177B (en) * | 2019-11-26 | 2021-12-28 | 济南大学 | Preparation method of electrochemical luminescence sensor for detecting procalcitonin |
| CN115108587A (en) * | 2022-06-24 | 2022-09-27 | 上海交通大学医学院附属仁济医院 | Molybdenum disulfide-doped two-dimensional carbon nitrogen compound matrix and preparation method and application thereof |
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Application publication date: 20160713 |
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