CN105758995A - Root box for submerged plant cultivation and rhizosphere microcell DGT test and use method thereof - Google Patents
Root box for submerged plant cultivation and rhizosphere microcell DGT test and use method thereof Download PDFInfo
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Abstract
The invention provides a root box for submerged plant cultivation and rhizosphere microcell DGT test and a use method thereof. Two filter screens are adopted for separating an inner chamber of a box body of the root box into a lower layer chamber, a middle layer chamber and an upper layer chamber which are arranged in turn from bottom to top; the roots of the submerged plant are cultivated in the middle layer chamber; the rhizosphere microcell sediments and the rootless sediments are effectively and obviously separated; and meanwhile, a test port, through which DGT and sediment sampler can pass, is formed on a side wall of the middle layer chamber; the in-situ and accurate determination of DGT for the elements, such as phosphorus, in the process of migrating on a plant root/ sediment interface can be realized; complete plant root characteristics can be acquired. The root box provided by the invention has the advantages that the structure is simple, the survival rate of the plants is high and the root box can be used for evaluating the bio-availability of the elements such as phosphorus. According to the use method of the root box provided by the invention, the cultivation for submerged plant and the in-situ test for rhizosphere microcell DGT can be realized at the same time, the operation is simple and convenient, and the use method can be easily large-scale popularized.
Description
Technical field
The present invention relates to water environment ecological restoration technical field, particularly relate to a kind of root case apparatus for element DGT in-situ tests such as submerged plant cultivation and plant rhizosphere microcell phosphorus and using method thereof.
Background technology
At present, the lake of China existing more than 80% is contaminated, and body eutrophication is on the rise, it has also become an overall situation problem currently urgently to be resolved hurrily, and for this, water body treating and water environment ecological reestablishment arise at the historic moment.Difference according to water body treating and Principles, this technology can be divided into Physical, chemical method and bio-ecological process three major types, wherein, bio-ecological restoration technology is developing progressively the technical way into Water Environmental Pollution Control because its cost is low, environmental benefit is high and repairing effect is good.In bio-ecological restoration technology, as planktonic algae competitor on nutrient substance and water power calculation, submerged plant can effectively suppress the growth of planktonic algae, the nutrient substance such as the nitrogen in water body, phosphorus can also be absorbed simultaneously, play and remove pollutant, accelerate the effect that Organic substance decomposes, therefore, the water plant recovery technique based on submerged plant is the hot research direction of current this area.
In prior art, the method for kind of planting submerged plant mainly has (1) fork planting method: utilize the bamboo pole with fork fork the cervical region of submerged plant and pitch in water；(2) kind of a method is thrown: by being manually thrown in water by submerged plant in hydrostatic；(3) gradually heavy formula planting method: by submerged plant planting on the carrier, the degree of depth that supporting body is sunk in water by matched elevation and subsidence regulating device control submerged plant.For above-mentioned implantation methods, owing to the visibility of contaminated water body is poor, when people is positioned on the ship of movement, fork planting method and throwing kind method all cannot control the planting density of submerged plant exactly, and the water body of flowing also can affect and throw the plant position that kind of method is planted, can produce after causing plant to sink under water to tilt even to fall on the bottom, thus affecting surviving of submerged plant；And gradually heavy formula planting method is generally cultivated on the culture base-material bottom planting unit because of submerged plant, the obstruct of culture base-material makes the water near riverbed and the dissolved oxygen amount in bed mud reduce, cause the breeding of anerobe, have a strong impact on water remediation effect, and existing planting unit generally also needs to add mixed bacteria in water body and to form biomembrane, water body is repaired, but this biomembranous purification specific aim is weak, the treatment effect of pollutant is poor, the introducing of external source strain is also unfavorable for water remediation, moreover the structure of planting unit is complicated, relatively costly, it is difficult to popularization and application, therefore just in the urgent need to existing gradually heavy formula planting unit is carried out structure improvement.
Additionally, owing to rhizosphere is the important area that crop root and soil carry out nutrient and energy exchange, it it is the most active place of soil microorganism, it is affected by crop root growth to affect again absorbing and the specific region of transhipment of crop nutrition content simultaneously, therefore, Rhizosphere is always up the focus of the multidisciplinary researches such as domestic and international agrology, Plant Nutrition, agronomy, the biological effectiveness disclosing submerged plant Rhizosphere phosphorus transfer and phosphorus is to recover and rebuild the key scientific problems that must solve in submerged vegetation, and still comparatively weak for the research of this respect at present.Although diffusive gradients in thin-films technology (DGT) can be used for the plant root in soil to the research in the DGT indicator of the phosphorus content of Transport And Transformation feature between plant rhizosphere/interstitial water/deposit of the absorption dynamics of phosphorus, phosphorus, prediction plant roots district phosphorus biological effectiveness and plant etc., but up to now, the Rhizosphere of submerged plant was never carried out test by DGT, also failed to for studying rhizosphere region to the mechanism of absorption of the elements such as phosphorus and biological usability.
Given this, how the DGT test of submerged plant Rhizosphere is combined with its root case planting unit, to realize directly in root case, submerged plant Rhizosphere being carried out DGT in-situ test, thus obtaining the elements such as the phosphorus that more tallies with the actual situation in the migration feature of rhizosphere region interface and the DGT parameter of root absorption mechanism and each such as plant, deposit, the concentration of element such as the phosphorus in interstitial water and DGT, it it is the key really grasping submerged plant root district to the element absorption such as phosphorus and Water Ecological Recovery mechanism, also it is the still unsolved technical barrier of this research field technical staff.
Summary of the invention
Technical problem solved by the invention is in that to overcome the structure complexity existing for existing submerged plant planting device, water remediation weak effect, cannot be carried out the defect of DGT in-situ test, and then provides a kind of simple in construction, water remediation effective and can carry out cultivating for submerged plant and the root case of Rhizosphere DGT test and using method thereof of DGT in-situ test.
For this, the present invention realizes the technical scheme of above-mentioned purpose and is:
A kind of root case for submerged plant cultivation and Rhizosphere DGT test, including:
Casing, the first filter screen and the second filter screen it is provided with in described casing, described first filter screen is positioned at the top of described second filter screen, described first filter screen and described second filter screen and the inner chamber of described casing is separated into the lower chamber, middle level chamber and the upper strata chamber that set gradually from the bottom to top；Wherein,
The open top of described upper strata chamber, arranges the through hole of some stem's traverses for submerged plant on described first filter screen；
Described middle level chamber, for cultivating the root of described submerged plant, is provided for DGT, detection mouth that deposit sampler passes through on the sidewall of described middle level chamber；
The bottom of described lower chamber is provided with the 3rd filter screen.
The ratio of described middle level chamber and the height of described lower chamber is 1:1～2:1.
The aperture of described first filter screen and described 3rd filter screen is respectively less than the granularity of the deposit for cultivating described submerged plant；Described first filter screen, the second filter screen and the 3rd filter screen fibreglass are made.
Also including DGT placing device, described DGT placing device includes conveying lever, is respectively provided with fixture and button at the both ends of described conveying lever, and the length direction along described conveying lever arranges the spring mechanism connecting described fixture and button.
Also including deposit sampler, it is the plastic injector without syringe needle, is used for extracting rhizodeposition matter sample；
It is being arranged in the described through hole in the middle part of described first filter screen and is being arranged to the probe of detection Rhizosphere pH value, Eh value and temperature.
Outside the sidewall of described casing, longitudinal scale is set along short transverse；Described conveying lever is provided with scale along the length direction of described conveying lever.
Also including a pair hanger, the pair of hanger is fixedly installed on the relative sidewall at described casing top；
Also including sealing lid, the described lid that seals is arranged at the outside of described detection mouth.
The using method of above-mentioned case, comprises the steps:
(1) in described lower chamber and described middle level chamber, add deposit, the root of described submerged plant is planted in the chamber of described middle level, and make the stem of described submerged plant extend in the chamber of described upper strata through described through hole；
(2) described case is fixed on the floating platform being built in studied waters, makes the distance from top water surface 0.5～1m of described case；
(3) after described submerged plant cultivates a period of time in described floating platform, the Rhizosphere of described submerged plant being tested, the step of described test includes:
S1, from described floating platform, take out described case；
S2, have on the circular DGT of the fine rule fixture being clamped in described DGT placing device by being, through described detection mouth, described circular DGT is sent in the rhizodeposition thing of described middle level chamber, press described button to be positioned in described rhizodeposition thing by described circular DGT, take out described DGT placing device, and the end of described fine rule is placed in outside described detection mouth；
S3, again the root case being placed with described circular DGT is fixed in described floating platform, so that described circular DGT carries out in-situ test in the Rhizosphere of described submerged plant, after described in-situ test completes, pulls described fine rule namely to can be taken off described circular DGT.
Described deposit prepares by the following method:
Gathering total phosphorus content is the bottom mud in lake of 3000mg/Kg, air-dry；
Gather the lake sand in not comtaminated waters, air-dry；
The ratio of the bottom mud in lake after air-dry with lake sand 1:14～14:1 in mass ratio is mixed, obtains described deposit.
Distance between placement location and the described detection mouth of described circular DGT is equal to the projection on described first filter screen of the described detection mouth and the distance between the center of described through hole；
Test, described in step (3), the step also including that rhizodeposition thing is sampled, particularly as follows: before carrying out described step S2, described deposit sampler is inserted in the rhizodeposition thing of described middle level chamber through described detection mouth, extracts rhizodeposition thing；
Also include being inserted in the rhizodeposition thing of described middle level chamber the described probe being used for detecting Rhizosphere pH value, Eh value and temperature.
The technique scheme of the present invention has the advantage that
1, root case for submerged plant cultivation and Rhizosphere DGT test provided by the invention, by adopting two filter screens that its cabinets cavity is separated into the lower chamber set gradually from the bottom to top, middle level chamber and upper strata chamber, and the root of submerged plant is cultivated in the chamber of middle level, thus effectively the deposit of Rhizosphere and unrooted deposit substantially being distinguished, the sidewall of the root case apparatus layer chamber wherein of the present invention is provided for DGT simultaneously, the detection mouth that deposit sampler passes through, it is advantageously implemented DGT to the elements such as the phosphorus original position at plant rhizosphere/Sediment Interface transition process, Accurate Determining, and complete plant root feature can also be obtained, so just can disclose submerged plant root more realistically to the dynamic absorption mechanism of the elements such as phosphorus and the ecological restoration function to nutrient laden water body in lake.
And, for the ease of carrying out DGT in-situ test, need to be fixed on floating platform by the root case apparatus of the present invention, the purpose of migration of element and mass exchange in order to simulate deposit/water/plant interface truly in addition again, so the present invention is at the lower chamber that has been arranged below of middle level chamber, and in lower chamber, also load deposit.
2, the root case for submerged plant cultivation and Rhizosphere DGT test provided by the invention, by outside the sidewall of its casing along short transverse arrange longitudinal scale and on conveying lever length direction along conveying lever scale is set, to be conducive to determining accurately the riding position of probe and DGT, thus realizing the test to submerged plant Rhizosphere more accurately.
Additionally, the root case apparatus of the present invention also has simple in construction, plant survival rate is high, water remediation is effective and can be used for the advantage that unit's bioavailabilities such as phosphorus are assessed.
3, the using method of of the present invention case, by the root of described submerged plant being planted in the deposit of described middle level chamber, and make the stem of described submerged plant extend in the chamber of described upper strata through described through hole, root box top is arranged on the distance water surface 0.5～1m place simultaneously, required illumination and nutrient can be provided on the one hand for surviving of submerged plant, be conducive to improving the survival rate of submerged plant, the reduction of the dissolved oxygen amount avoided near root case in water environment on the other hand, and due to adopt the present invention root case cultivate submerged plant time without additionally adding strain, it is thus possible to guarantee the submerged plant repairing effect to water body.
It addition, the root case using method of the present invention can synchronize to realize the in-situ test of the cultivation to submerged plant and Rhizosphere DGT, there is advantage easy and simple to handle, to be prone to large-scale promotion.
Accompanying drawing explanation
In order to be illustrated more clearly that the technical scheme in the specific embodiment of the invention, below the accompanying drawing used required during detailed description of the invention is described is briefly described, apparently, accompanying drawing in the following describes is some embodiments of the present invention, for those of ordinary skill in the art, under the premise not paying creative work, it is also possible to obtain other accompanying drawing according to these accompanying drawings.
Fig. 1 is the structural representation of the root case apparatus of the present invention；
Fig. 2 is the structural representation of the DGT placing device in the present invention.
Wherein, accompanying drawing labelling is as follows:
1-casing；2-the first filter screen；3-the second filter screen；4-lower chamber；5-middle level chamber；6-upper strata chamber；7-through hole；8-detects mouth；9-the 3rd filter screen；10-conveying lever；11-fixture；12-button；13-circle DGT；14-hanger；15-submerged plant.
Detailed description of the invention
Below in conjunction with accompanying drawing, technical scheme is clearly and completely described, it is clear that described embodiment is a part of embodiment of the present invention, rather than whole embodiments.Based on the embodiment in the present invention, the every other embodiment that those of ordinary skill in the art obtain under not making creative work premise, broadly fall into the scope of protection of the invention.As long as just can be combined with each other additionally, technical characteristic involved in invention described below difference embodiment does not constitute conflict each other.
Embodiment 1
As it is shown in figure 1, cultivating and the root case of Rhizosphere DGT test for submerged plant described in the present embodiment, including:
Casing 1, the first filter screen 2 and the second filter screen 3 it is provided with in described casing 1, described first filter screen 2 is positioned at the top of described second filter screen 3, described first filter screen 2 and described second filter screen 3 and the inner chamber of described casing 1 is separated into the lower chamber 4, middle level chamber 5 and the upper strata chamber 6 that set gradually from the bottom to top；Wherein, the open top of described upper strata chamber 6, described first filter screen 1 is provided with the through hole 7 of 3 stem's traverses being available for submerged plant；Described middle level chamber 5, for cultivating the root of described submerged plant, is provided for DGT and detection mouth 8 that deposit sampler passes through on the sidewall of described middle level chamber 5；The bottom of described lower chamber 4 is provided with the 3rd filter screen 9；In the present embodiment, whole case is made up of opaque PVC plastic, it is an internal diameter is 26cm, the high cylinder for 50cm, the height of described lower chamber 4 and described middle level chamber 5 is 15cm, the aperture of described first filter screen 2 and described 3rd filter screen 9 is 28 μm, less than being used for cultivating the granularity of the deposit of described submerged plant, the aperture of described second filter screen 3 is 1mm, and described first filter screen the 2, second filter screen 3 and the 3rd filter screen 9 fibreglass in the present embodiment is made.
Root case apparatus in the present embodiment is by cultivating the root of submerged plant in the chamber of middle level, effectively the soil of Rhizosphere and unrooted soil are substantially distinguished, simultaneously the sidewall of the root case apparatus layer chamber wherein of the present invention is provided for DGT, detection mouth that deposit sampler passes through, it is advantageously implemented DGT to elements such as phosphorus at the plant rhizosphere/original position of Sediment Interface transition process, Accurate Determining, so just can disclose submerged plant root more realistically to the dynamic absorption mechanism of the elements such as phosphorus and the ecological restoration function to nutrient laden water body in lake.Additionally, the advantage that the root case apparatus of the present invention also has simple in construction, plant survival rate is high and water remediation is effective.
The using method of the root case in the present embodiment, comprises the steps:
(1) gathering total phosphorus content in the region that Lake Erhai Eutrophic Extent is higher is the bottom mud in lake of 3000mg/Kg, air-dry；Gather the lake sand in the better waters of unpolluted water quality, air-dry；Bottom mud in lake after air-dry is mixed with the ratio that lake sand difference is 1:14～14:1 in mass ratio, it is thus achieved that there is the sequence of deposition thing 14 parts of total phosphorus concentration gradient；
(2) in the lower chamber of described case and middle level chamber, add the deposit of step (1), each described case adds 1 part of deposit, the root of spike watermifoil is planted in the chamber of described middle level, and makes the stem of spike watermifoil extend in the chamber of described upper strata through described through hole；
(3) described case is fixed on be built on Erhai close water plant Experimental Base floating platform on, make the distance from top water surface 0.5m of described case；
(4) after spike watermifoil cultivates a growth cycle in described floating platform, the Rhizosphere of spike watermifoil being tested, the step of described test includes:
S1, from described floating platform, take out described case；
S2, have on the circular DGT of the fine rule fixture being clamped in described DGT placing device by being, through described detection mouth, described circular DGT is sent in the rhizodeposition thing of described middle level chamber, press described button to be positioned in described rhizodeposition thing by described circular DGT, take out described DGT placing device, the end of described fine rule is placed in outside described detection mouth；
S3, again the root case being placed with described circular DGT is fixed in described floating platform, so that described circular DGT carries out in-situ test in the Rhizosphere of spike watermifoil, complete after (it is generally required to 24 hours) until described in-situ test, pull described fine rule namely to can be taken off described circular DGT.
The using method of the root case described in the present embodiment, by the root of spike watermifoil being planted in the rhizodeposition thing of described middle level chamber, and make the stem of spike watermifoil extend in the chamber of described upper strata through described through hole, root box top is arranged on distance water surface 0.5m place simultaneously, required illumination and nutrient can be provided on the one hand for surviving of submerged plant spike watermifoil, be conducive to improving the survival rate of submerged plant, the reduction of the dissolved oxygen amount avoided near root case in water environment on the other hand, and due to adopt the present invention root case cultivate spike watermifoil time without additionally adding strain, it is thus possible to guarantee the spike watermifoil repairing effect to water body.It addition, the root case using method of the present embodiment can synchronize to realize the in-situ test of the cultivation to spike watermifoil and Rhizosphere DGT, there is advantage easy and simple to handle, to be prone to large-scale promotion.
Embodiment 2
As it is shown in figure 1, cultivating and the root case of Rhizosphere DGT test for submerged plant described in the present embodiment, including:
Casing 1, outside the sidewall of described casing 1, longitudinal scale is set along short transverse, the first filter screen 2 and the second filter screen 3 it is provided with in described casing 1, described first filter screen 2 is positioned at the top of described second filter screen 3, described first filter screen 2 and described second filter screen 3 and the inner chamber of described casing 1 is separated into the lower chamber 4, middle level chamber 5 and the upper strata chamber 6 that set gradually from the bottom to top；Wherein,
The open top of described upper strata chamber 6, arranges the through hole 7 of 5 stem's traverses being available for submerged plant on described first filter screen 1, and is being arranged in the described through hole 7 in the middle part of described first filter screen 1 and is arranged to the probe of detection Rhizosphere pH value, Eh value and temperature；
Described middle level chamber 5, for cultivating the root of described submerged plant, is provided for DGT on the sidewall of described middle level chamber 5 and detection mouth 8 that deposit sampler passes through, is provided with sealing lid in the outside of described detection mouth 8；
The bottom of described lower chamber 4 is provided with the 3rd filter screen 9；
DGT placing device, refer to Fig. 2, including conveying lever 10, described conveying lever 10 is provided with scale along the length direction of described conveying lever 10, be respectively provided with fixture 11 and button 12 at the both ends of described conveying lever 10, the length direction along described conveying lever 10 arranges the spring mechanism connecting described fixture 11 and button 12；
A pair hanger 14, the pair of hanger 14 is fixedly installed on the relative sidewall at described casing 1 top.
In the present embodiment, whole case is made up of opaque PVC plastic, it is an internal diameter is 26cm, the high cylinder for 50cm, height respectively 15cm, 30cm of described lower chamber 4 and described middle level chamber 5, the aperture of described first filter screen 2 and described 3rd filter screen 9 is 28 μm, less than being used for cultivating the granularity of the deposit of described submerged plant, the aperture of the second filter screen 3 is 1mm, and described first filter screen the 2, second filter screen 3 and the 3rd filter screen 9 fibreglass in the present embodiment is made.Electrode probe (thermoelectricity Ao Lilong company, the U.S.) that probe used by the present embodiment is the portable pH/Eh of 5-Star and the probe (Wuxi Gao Cheng precision instrument company limited) of soil temperature data harvester.
The using method of the root case in the present embodiment, comprises the steps:
(1) gathering total phosphorus content in the region that Lake Erhai Eutrophic Extent is higher is the bottom mud in lake of 3000mg/Kg, air-dry；Gather the lake sand in the better waters of unpolluted water quality, air-dry；Bottom mud in lake after air-dry is mixed with the ratio that lake sand difference is 14:1～1:14 in mass ratio, it is thus achieved that there is the sequence of deposition thing 14 parts of total phosphorus concentration gradient；
(2) in the lower chamber of described case and middle level chamber, add the deposit of step (1), each described case adds 1 part of deposit, the root of spike watermifoil is planted in the chamber of described middle level, and makes the stem of spike watermifoil extend in the chamber of described upper strata through described through hole；The described probe being used for detecting Rhizosphere pH value, Eh value and temperature is inserted in the rhizodeposition thing of described middle level chamber；
(3) hawser is set on the pair of hanger 14, by described hawser described case is fixed on and is built on Erhai and closes on the floating platform of water plant Experimental Base, make the distance from top water surface 1m of described case；
(4) after spike watermifoil cultivates a growth cycle in described floating platform, the Rhizosphere of spike watermifoil being tested, the step of described test includes:
S1, from described floating platform, take out described case, open and described seal lid；
S2, deposit is taken sample insert in the rhizodeposition thing of described middle level chamber through described detection mouth, extract rhizodeposition thing, obtain rhizodeposition matter sample；
S3, have on the circular DGT of the fine rule fixture being clamped in described DGT placing device by being, through described detection mouth, described circular DGT is sent in the rhizodeposition thing of described middle level chamber, press described button to be positioned in described rhizodeposition thing by described circular DGT, and guarantee that the distance between placement location and the described detection mouth of described circular DGT is equal to the projection on described first filter screen of the described detection mouth and the distance between the center of described through hole；Then take out described DGT placing device, the end of described fine rule is placed in outside described detection mouth, close described sealing and cover；
S4, again the root case being placed with described circular DGT is fixed on described floating platform, so that described circular DGT carries out in-situ test in the Rhizosphere of spike watermifoil, after described in-situ test completes, pulls described fine rule namely to can be taken off described circular DGT.
Obviously, above-described embodiment is only for clearly demonstrating example, and is not the restriction to embodiment.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here without also cannot all of embodiment be given exhaustive.And the apparent change thus extended out or variation are still among the protection domain of the invention.
Claims (10)
1. cultivate and a root case for Rhizosphere DGT test for submerged plant, including:
Casing, the first filter screen and the second filter screen it is provided with in described casing, described first filter screen is positioned at the top of described second filter screen, described first filter screen and described second filter screen and the inner chamber of described casing is separated into the lower chamber, middle level chamber and the upper strata chamber that set gradually from the bottom to top；Wherein,
The open top of described upper strata chamber, arranges the through hole of some stem's traverses for submerged plant on described first filter screen；
Described middle level chamber, for cultivating the root of described submerged plant, is provided for DGT, detection mouth that deposit sampler passes through on the sidewall of described middle level chamber；
The bottom of described lower chamber is provided with the 3rd filter screen.
2. according to claim 1 case, it is characterised in that the ratio of described middle level chamber and the height of described lower chamber is 1:1～2:1.
3. according to claim 1 and 2 case, it is characterised in that the aperture of described first filter screen and described 3rd filter screen is respectively less than the granularity of the deposit for cultivating described submerged plant；Described first filter screen, the second filter screen and the 3rd filter screen fibreglass are made.
4. the root case according to any one of claim 1-3, it is characterized in that, also include DGT placing device, described DGT placing device includes conveying lever, be respectively provided with fixture and button at the both ends of described conveying lever, the length direction along described conveying lever arranges the spring mechanism connecting described fixture and button.
5. the root case according to any one of claim 1-4, it is characterised in that also including deposit sampler, it is the plastic injector without syringe needle, is used for extracting rhizodeposition matter sample；
It is being arranged in the described through hole in the middle part of described first filter screen and is being arranged to the probe of detection Rhizosphere pH value, Eh value and temperature.
6. the root case according to any one of claim 1-5, it is characterised in that outside the sidewall of described casing, longitudinal scale is set along short transverse；Described conveying lever is provided with scale along the length direction of described conveying lever.
7. the root case according to any one of claim 1-6, it is characterised in that also include a pair hanger, the pair of hanger is fixedly installed on the relative sidewall at described casing top；
Also including sealing lid, the described lid that seals is arranged at the outside of described detection mouth.
8. the using method of root case described in any one of claim 1-7, comprises the steps:
(1) in described lower chamber and described middle level chamber, add deposit, the root of described submerged plant is planted in the chamber of described middle level, and make the stem of described submerged plant extend in the chamber of described upper strata through described through hole；
(2) described case is fixed on the floating platform being built in studied waters, makes the distance from top water surface 0.5～1m of described case；
(3) after described submerged plant cultivates a period of time in described floating platform, the Rhizosphere of described submerged plant being tested, the step of described test includes:
S1, from described floating platform, take out described case；
S2, have on the circular DGT of the fine rule fixture being clamped in described DGT placing device by being, through described detection mouth, described circular DGT is sent in the rhizodeposition thing of described middle level chamber, press described button to be positioned in described rhizodeposition thing by described circular DGT, take out described DGT placing device, and the end of described fine rule is placed in outside described detection mouth；
S3, again the root case being placed with described circular DGT is fixed in described floating platform, so that described circular DGT carries out in-situ test in the Rhizosphere of described submerged plant, after described in-situ test completes, pulls described fine rule namely to can be taken off described circular DGT.
9. the using method of according to claim 8 case, it is characterised in that described deposit prepares by the following method:
Gathering total phosphorus content is the bottom mud in lake of 3000mg/Kg, air-dry；
Gather the lake sand in not comtaminated waters, air-dry；
The ratio of the bottom mud in lake after air-dry with lake sand 1:14～14:1 in mass ratio is mixed, obtains described deposit.
10. the using method of root case according to claim 8 or claim 9, it is characterised in that the distance between placement location and the described detection mouth of described circular DGT is equal to the projection on described first filter screen of the described detection mouth and the distance between the center of described through hole；
Test, described in step (3), the step also including that rhizodeposition thing is sampled, particularly as follows: before carrying out described step S2, described deposit sampler is inserted in the rhizodeposition thing of described middle level chamber through described detection mouth, extracts rhizodeposition thing；
Also include being inserted in the rhizodeposition thing of described middle level chamber the described probe being used for detecting Rhizosphere pH value, Eh value and temperature.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109406244A (en) * | 2018-12-29 | 2019-03-01 | 中国科学院南京地理与湖泊研究所 | A kind of water plant root exudates original position collection device |
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