CN1057525A - A kind ofly measure the solid reagent that total serum protein is used - Google Patents
A kind ofly measure the solid reagent that total serum protein is used Download PDFInfo
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- CN1057525A CN1057525A CN 91100625 CN91100625A CN1057525A CN 1057525 A CN1057525 A CN 1057525A CN 91100625 CN91100625 CN 91100625 CN 91100625 A CN91100625 A CN 91100625A CN 1057525 A CN1057525 A CN 1057525A
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- reagent
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- total serum
- serum protein
- sodium
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Abstract
What the present invention relates to is a kind of solid reagent that total serum protein is used of measuring, and its principal ingredient is copper sulphate, sodium potassium tartrate tetrahydrate, sodium phosphate, sodium azide and non-ionic surfactant.Solid reagent of the present invention, its weight and volume all is significantly smaller than the liquid biuret reagent, and is simple to operate, easy to use, is colour-stable, is convenient to packing, transportation and storage, is used to put into practice good accuracy and precision.
Description
What the present invention relates to is a kind of solid reagent that total serum protein is used of measuring.
Total serum protein has important value to the diagnosis and the auxiliary diagnosis of human body diseases.Total serum protein raises and is common in acute fluid loss property diseases such as vomiting, diarrhoea, Huppert's disease and hyposupraenalism etc.; Total protein concentration of serum reduces can be by the protein insufficiency of intake, and serious deeline such as tuberculosis, hyperthyroidism, tumour etc. cause, the liver function grievous injury, and it is low that diseases such as nephrotic syndrome such as ulcerative colitis all can show as total protein concentration.
At present, quilt reagent clinical both at home and abroad and the mensuration total serum protein that extensively also adopt for a long time in the laboratory is biuret reagent, its principal ingredient is a copper sulphate, sodium potassium tartrate tetrahydrate and NaOH, the also adding potassium iodide that has, during by the conventional formulation preparation, must be with copper sulphate, sodium potassium tartrate tetrahydrate and NaOH dissolve respectively, then sodium potassium tartrate tetrahydrate is mixed with copper sulphate, add concentration again and be 30% sodium hydroxide solution, then may produce turbidity sediment as not following this step, even reagent can not be used, NaOH in the prescription is graininess, corrosivity and hydroscopicity are all extremely strong, so, up to now, be that domestic or external reagent producer makes liquid form with reagent to offer the user, but the liquid reagent volume is big, weight is big, easy to leak, be difficult to the guarantee reagent quality, and the trucking costs height, the packing difficulty increased.
The object of the present invention is to provide a kind of solid reagent that total serum protein is used of measuring, make it not only easy to use, and its weight and volume all is significantly smaller than liquid reagent, be easy to packing, be convenient to store and transportation, guaranteed that the stable storage of reagent and preparation are easily molten.
The object of the present invention is achieved like this, after having carried out a large amount of experiment screenings, having selected principal ingredient for use is the solid reagent of copper sulphate, sodium potassium tartrate tetrahydrate, sodium phosphate, sodium azide and non-ionic surfactant, and its solution ratio is: in every liter of reagent solution, contain:
Copper sulphate 2～4 grams per liters
Sodium potassium tartrate tetrahydrate 4～8 grams per liters
Sodium phosphate 10～20 grams per liters
Sodium azide 0.5～1 grams per liter
Non-ionic surfactant:
Triton X-100(Triton X-100)
Or Tween 20(Tween-20)
Or Tween 80(polyethoxy oleic acid D-sorbite) 2～4 grams per liters
Or Brij 35(polyoxyethylene laurel ether) 1～3 grams per liter
Wherein the non-ionic surfactant of Jia Ruing is for making the unlikely generation of solution muddy, and sodium azide then is as antiseptic.
Work in-process, we are divided into two groups with above-mentioned five kinds of materials, that is:
Component A: copper sulphate and sodium azide
B component: sodium potassium tartrate tetrahydrate, sodium phosphate and non-ionic surfactant
When making finished product, only need proportioning mixing in accordance with regulations with component A, get final product packing, be finished product; B component then takes by weighing by weight after the abundant mixing of electronic stirring is moistening Powdered at this moment, and powder was placed vacuum dryer at least 24 hours, and stirring and evenly mixing gets final product packing once more, is finished product.Only need during use will ask for quantitative component A and B component in proportion respectively with dissolved in distilled water, mixing can use then.
Reagent weight and volume of the present invention is all well below liquid reagent, 1000 milliliters of weight of primary liquid reagent, 1027 grams, 1000 milliliters of consumptions of solid reagent are 29 grams only, 30 milliliters of volumes, its weight and volume dwindles 35 times and 34 times than liquid reagent respectively, convenient packing, transportation; Adopt sodium phosphate to replace the extremely strong NaOH of corrosivity in the prescription, helped safe operation; The floccus that suspends just appears in primary liquid reagent often some months, influence is used, even newly purchasing in the reagent (Beijing Chemical Plant's product) of having has tangible floccus, the evidence solid reagent is under solid state, at least can guarantee the quality 1 year, be mixed with behind the liquid six months still limpid, period of storage is long.For obtaining more consistent test result with liquid reagent, we have carried out experiment relatively with the biuret reagent as commodity selling that the Beijing Chemical Plant produces as contrast, discovery changes 4 milliliters of original reagent amount into 3 milliliters can reach the detection requirement, in other words, can save 1/4th amount of reagent, help reducing the chemical examination cost.Utilize product of the present invention to detect, simple to operate, rapid, have good accuracy and precision.
Embodiment:
Get component A copper sulphate 1 gram
Sodium azide 0.25 gram
B component sodium potassium tartrate tetrahydrate 3 grams
Sodium phosphate 10 grams
Non-ionic surfactant:
Tween 80(polyethoxy oleic acid D-sorbite) 2 grams
Or Brij 35(polyoxyethylene laurel ether) 1 gram
With component A mixing, stand-by, B component is mixed after electric blender stirs, fully mixing places vacuum dryer decompression 24 hours with the moistening powder behind the mixed spoon, takes out stirring and evenly mixing once more, and is stand-by; Component A and B component respectively with 100 milliliters and the dissolving of 200 ml distilled waters, are slowly added component A in the B component while stirring, and firmly jolting adds water to 500 milliliters of mixings again, and it is standby to bottle.
Serum 50 microlitres will be put in addition in the testing tube, put into titer 50 microlitres in the standard pipe, and at above-mentioned testing tube, standard pipe with got in addition and respectively put into 3 milliliters of reagent in the test tube, with each pipe mixing, 37 ℃ of water-baths 20 minutes are taken out and are placed room temperature cooling 5 minutes, use wavelength 628nm, the cuvette of 1cm, blank pipe zeroising carries out colorimetric.
Total protein concentration of serum (g/dl)=(testing tube OD)/(standard pipe OD) * concentration of standard solution (g/dl)
Normal reference value is 6～8g/dl
Solid reagent quality index of the present invention is:
Precision is criticized interior (n=10) SD=0.090 CV=1.29%
(n=25) SD=0.094 CV=1.31% between crowd
The accuracy recovery 97.6%
The range of linearity 2～10g/dl
60 minutes stabilization time behind the colour generation
1 year solid state shelf-life (the airtight preservation of room temperature)
Half a year liquid condition shelf-life (the airtight preservation of room temperature)
Claims (2)
1, a kind ofly measures the solid reagent that total serum protein is used, it is characterized in that: the solid reagent that it is made up of copper sulphate, sodium potassium tartrate tetrahydrate, sodium phosphate, sodium azide and non-ionic surfactant.
2, a kind of reagent that total serum protein is used of measuring according to claim 1, it is characterized in that: the component content behind the wiring solution-forming in every liter is as follows:
Copper sulphate 2～4 grams per liters
Sodium potassium tartrate tetrahydrate 4～8 grams per liters
Sodium phosphate 10～20 grams per liters
Sodium azide 0.5～1 grams per liter
Non-ionic surfactant:
Triton X-100(Triton X-100)
Or Tween 20(Tween-20)
Or Tween 80(polyethoxy oleic acid D-sorbite) 2～4 grams per liters
Or Brij 35(polyoxyethylene laurel ether) 1～3 grams per liter
Distilled water surplus
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 91100625 CN1057525A (en) | 1991-02-05 | 1991-02-05 | A kind ofly measure the solid reagent that total serum protein is used |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 91100625 CN1057525A (en) | 1991-02-05 | 1991-02-05 | A kind ofly measure the solid reagent that total serum protein is used |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1057525A true CN1057525A (en) | 1992-01-01 |
Family
ID=4904731
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 91100625 Pending CN1057525A (en) | 1991-02-05 | 1991-02-05 | A kind ofly measure the solid reagent that total serum protein is used |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1057525A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106442352A (en) * | 2016-09-24 | 2017-02-22 | 济南中安生物技术服务有限公司 | Total serum protein detection kit with strong anti-interference capability |
| CN107525774A (en) * | 2016-06-21 | 2017-12-29 | 山东博科生物产业有限公司 | Potassium hydroxide method total protein diagnostic test kits |
| CN114755195A (en) * | 2022-06-14 | 2022-07-15 | 浙江省立同德医院(浙江省精神卫生研究院) | Preparation method of reagent for quickly detecting urine protein |
-
1991
- 1991-02-05 CN CN 91100625 patent/CN1057525A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107525774A (en) * | 2016-06-21 | 2017-12-29 | 山东博科生物产业有限公司 | Potassium hydroxide method total protein diagnostic test kits |
| CN106442352A (en) * | 2016-09-24 | 2017-02-22 | 济南中安生物技术服务有限公司 | Total serum protein detection kit with strong anti-interference capability |
| CN114755195A (en) * | 2022-06-14 | 2022-07-15 | 浙江省立同德医院(浙江省精神卫生研究院) | Preparation method of reagent for quickly detecting urine protein |
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| WD01 | Invention patent application deemed withdrawn after publication |