A kind of lily seed bulb scale breeds seed ball method
Technical field
The present invention relates to a kind of lily ball mating system, particularly a kind of lily seed bulb scale breeds seed ball method.
Background technology
Bulbus Lilii is unifacial leaf guiding principle Liliaceae, the perennial bulb herbaceous plant of lilium, for naked bulb ground light soy sauce stem type flowering bulb, although China's Bulbus Lilii aboundresources, the whole world has lily 94 kinds, what wherein originate in China reaches 47 kinds, but is still in the starting stage owing to China's lily ball produces, therefore wants about 1.8 hundred million, imported lily corm kind ball every year.Along with the development of market economy, the demand of lily ball is also substantially increased.But, the problems such as the kind ball of Bulbus Lilii is easily degenerated, pest and disease damage seriously govern production and the market demand of Bulbus Lilii.
The main path that current Bulbus Lilii breeds has: 1, the natural propagation of bulb, and Bulbus Lilii, after annual growth, defines new bulb at underground part, various types of bulbs all can be split by its natural form, breed.The bulb volume of this modes of reproduction is big, if the suitable good new individuality of blooming that can grow up to of condition, but limited amount, it is impossible to become the main method of breeding kind ball;2, the breeding of seed ball, as the stem-roots such as Lilium longiflorum can grow seed ball close to ground place, after fully growing up, can take off and plant, but measure little effort;3, bulbil breeding, the Bulbus Liliies such as tiger lily can rush and grow atropurpureus bulbil in stipes axil, bulbil is made up of scale several pieces plump, it can naturally be taken root and come into leaves, after fully growing up, it is possible to naturally come off, if the strain bud come off is planted in soil, will soon form little plant, but it is all few to breed kind, amount;4, tissue culture propagating, though tissue culture can well reach detoxification, purpose numerous soon, but investment of production is big, and technical merit, cost are high.During Bulbus Lilii breeds relatively above, scale lining is the way that the breeding coefficient commonly used in asexual propagation is the highest, scale raising technology is to solve one of main path of breeding of lily ball, lily bud scale strips rear quantity up to 12-26 sheet, when breeding, the base portion of every a piece of scale can produce 1-6 grain seed ball, after fully growing up, can plant, but in existing reproductive process, ubiquity scale rotting rate bulbec high, little regenerates, breeds the situations such as low.
Summary of the invention
The technical problem to be solved in the present invention is for the deficiencies in the prior art, it is proposed to a kind of small investment, cost are low, and the lily seed bulb scale that can improve yield breeds seed ball method.
The technical problem to be solved in the present invention is achieved through the following technical solutions.The present invention is that a kind of lily seed bulb scale breeds seed ball method, is characterized in, comprises the following steps: choosing of (1) lily ball: choose smooth surface, without mouldy, without rotting, girth is at the lily ball of 12-24cm;(2) ball sterilization is planted: put into by the lily ball chosen in step (1) in broad-spectrum germicide 10-20 minute, then being diluted in 2000-4000 times of potassium permanganate diluent 1-5 minute being placed in, described broad-spectrum germicide is be diluted to the methyl isophthalic acid H-2-benzimidazole carbamate solution of 1000-1500 times;(3) breaking dormancy: the lily ball after step (2) being sterilized shelters from heat or light after ventilation 5-8 hour, puts into the freezer that design temperature is-1 DEG C to-2 DEG C and gets final product breaking dormancy in 40-60 days;(4) dormancy transition: after breaking kind of ball dormancy, temperature of ice house is transferred to 2-4 DEG C;(5) lily bud scale strips: lily bud scale is peeled off from lily ball foundation portion ecto-entad by the lily ball obtained after step (4), strips 6-8 layer;(6) rear wound healing is stripped: the lily bud scale after peeling off is put into 3-6 DEG C, and ventilation promotes wound to heal for 5-7 days;(7) grass carbon is puddled: will be diluted with water to 500-1500 times of broad-spectrum germicide methyl isophthalic acid H-2-benzimidazole carbamate and be stirred with Pin Shi Top of Denmark turfy soil 1:5000 ratio;(8) epidemic disaster management and control: Pin Shi Top of Denmark turfy soil and the lily bud scale after step (7) being stirred carries out after 2:1 puddles, put into and lay in the high density polyethylene (HDPE) frame with aseptic polyethylene film, described aseptic polyethylene film is provided with air-vent, and air-vent aperture is 3 millimeters;Above-specified high density polyethylene frame is put into temperature be 20-25 DEG C, air humidity be the thermostatic chamber of 60%, heat 50-60 days.
One lily seed bulb scale of the present invention is bred in seed ball method and technology scheme, it is preferred that technical scheme be characterized by:
1, comprise the following steps: choosing of (1) lily ball: choose smooth surface, without mouldy, without rotting, girth is at the lily ball of 20cm;(2) ball sterilization is planted: put into by the lily ball chosen in step (1) in broad-spectrum germicide 15 minutes, then being diluted in 3000 times of potassium permanganate diluents 2 minutes being placed in, described broad-spectrum germicide is be diluted to the methyl isophthalic acid H-2-benzimidazole carbamate solution of 1200 times;(3) breaking dormancy: the lily ball after step (2) sterilization is sheltered from heat or light after ventilating 5 hours, put into the freezer that design temperature is-1 DEG C and get final product breaking dormancy in 40 days;(4) dormancy transition: after breaking kind of ball dormancy, temperature of ice house is transferred to 3 DEG C;(5) lily bud scale strips: lily bud scale is peeled off from lily ball foundation portion ecto-entad by the lily ball obtained after step (4), strips 7 layers;(6) rear wound healing is stripped: the lily bud scale after peeling off is put into 5 DEG C, ventilates and promote wound to heal in 6 days;(7) grass carbon is puddled: will be diluted with water to 1000 times of broad-spectrum germicide methyl isophthalic acid H-2-benzimidazole carbamates and be stirred with Pin Shi Top of Denmark turfy soil 1:5000 ratio;(8) epidemic disaster management and control: Pin Shi Top of Denmark turfy soil and the lily bud scale after step (7) being stirred carries out after 2:1 puddles, put into and lay in the high density polyethylene (HDPE) frame with aseptic polyethylene film, described aseptic polyethylene film is provided with air-vent, and air-vent aperture is 3 millimeters;Above-specified high density polyethylene frame is put into temperature be 23 DEG C, air humidity be the thermostatic chamber of 60%, heat 55 days;
2, comprise the following steps: choosing of (1) lily ball: choose smooth surface, without mouldy, without rotting, girth is at the lily ball of 12cm;(2) ball sterilization is planted: put into by the lily ball chosen in step (1) in broad-spectrum germicide 10 minutes, then being diluted in 2000 times of potassium permanganate diluents 1 minute being placed in, described broad-spectrum germicide is be diluted to the methyl isophthalic acid H-2-benzimidazole carbamate solution of 1000 times;(3) breaking dormancy: the lily ball after step (2) sterilization is sheltered from heat or light after ventilating 5 hours, put into the freezer that design temperature is-1 DEG C and get final product breaking dormancy in 40 days;(4) dormancy transition: after breaking kind of ball dormancy, temperature of ice house is transferred to 2 DEG C;(5) lily bud scale strips: lily bud scale is peeled off from lily ball foundation portion ecto-entad by the lily ball obtained after step (4), strips 6 layers;(6) rear wound healing is stripped: the lily bud scale after peeling off is put into 3 DEG C, ventilates and promote wound to heal in 5 days;(7) grass carbon is puddled: will be diluted with water to 500 times of broad-spectrum germicide methyl isophthalic acid H-2-benzimidazole carbamates and be stirred with Pin Shi Top of Denmark turfy soil 1:5000 ratio;(8) epidemic disaster management and control: Pin Shi Top of Denmark turfy soil and the lily bud scale after step (7) being stirred carries out after 2:1 puddles, put into and lay in the high density polyethylene (HDPE) frame with aseptic polyethylene film, described aseptic polyethylene film is provided with air-vent, and air-vent aperture is 3 millimeters;Above-specified high density polyethylene frame is put into temperature be 20 DEG C, air humidity be the thermostatic chamber of 60%, heat 50 days;
3, comprise the following steps: choosing of (1) lily ball: choose smooth surface, without mouldy, without rotting, girth is at the lily ball of 24cm;(2) ball sterilization is planted: put into by the lily ball chosen in step (1) in broad-spectrum germicide 20 minutes, then being diluted in 4000 times of potassium permanganate diluents 5 minutes being placed in, described broad-spectrum germicide is be diluted to the methyl isophthalic acid H-2-benzimidazole carbamate solution of 1500 times;(3) breaking dormancy: the lily ball after step (2) sterilization is sheltered from heat or light after ventilating 8 hours, put into the freezer that design temperature is-2 DEG C and get final product breaking dormancy in 60 days;(4) dormancy transition: after breaking kind of ball dormancy, temperature of ice house is transferred to 4 DEG C;(5) lily bud scale strips: lily bud scale is peeled off from lily ball foundation portion ecto-entad by the lily ball obtained after step (4), strips 8 layers;(6) rear wound healing is stripped: the lily bud scale after peeling off is put into 6 DEG C, ventilates and promote wound to heal in 7 days;(7) grass carbon is puddled: will be diluted with water to 1500 times of broad-spectrum germicide methyl isophthalic acid H-2-benzimidazole carbamates and be stirred with Pin Shi Top of Denmark turfy soil 1:5000 ratio;(8) epidemic disaster management and control: Pin Shi Top of Denmark turfy soil and the lily bud scale after step (7) being stirred carries out after 2:1 puddles, put into and lay in the high density polyethylene (HDPE) frame with aseptic polyethylene film, described aseptic polyethylene film is provided with air-vent, and air-vent aperture is 3 millimeters;Above-specified high density polyethylene frame is put into temperature be 25 DEG C, air humidity be the thermostatic chamber of 60%, heat 60 days;
4, the thickness of described aseptic polyethylene film 0.04 millimeter.
Compared with prior art, cost of the present invention is low, yield is high, comprise the following steps the choosing of lily ball, plant ball sterilization, breaking dormancy, dormancy transition, lily bud scale strip, strip rear wound healing, grass carbon is puddled and epidemic disaster management and control, being undertaken that lily ball breeds by the method can at short notice by 22,000,000 seed ball of Bulbus Lilii mother bulb fast-propagation to 18,000,000 of 300,000 originally, improve the yield bred, broken external lily ball seed ball and be prohibited from entering the monopolization of China.
Detailed description of the invention
Further describe the concrete technical scheme of the present invention, in order to those skilled in the art is further understood that the present invention, and does not constitute the restriction of its power.
Embodiment 1, a kind of lily seed bulb scale breeds seed ball method, comprises the following steps: choosing of (1) lily ball: choose smooth surface, without mouldy, without rotting, girth is at the lily ball of 12-24cm;(2) ball sterilization is planted: put into by the lily ball chosen in step (1) in broad-spectrum germicide 10-20 minute, then being diluted in 2000-4000 times of potassium permanganate diluent 1-5 minute being placed in, described broad-spectrum germicide is be diluted to the methyl isophthalic acid H-2-benzimidazole carbamate solution of 1000-1500 times;(3) breaking dormancy: the lily ball after step (2) being sterilized shelters from heat or light after ventilation 5-8 hour, puts into the freezer that design temperature is-1 DEG C to-2 DEG C and gets final product breaking dormancy in 40-60 days;(4) dormancy transition: after breaking kind of ball dormancy, temperature of ice house is transferred to 2-4 DEG C;(5) lily bud scale strips: lily bud scale is peeled off from lily ball foundation portion ecto-entad by the lily ball obtained after step (4), strips 6-8 layer;(6) rear wound healing is stripped: the lily bud scale after peeling off is put into 3-6 DEG C, and ventilation promotes wound to heal for 5-7 days;(7) grass carbon is puddled: will be diluted with water to 500-1500 times of broad-spectrum germicide methyl isophthalic acid H-2-benzimidazole carbamate and be stirred with Pin Shi Top of Denmark turfy soil 1:5000 ratio;(8) epidemic disaster management and control: Pin Shi Top of Denmark turfy soil and the lily bud scale after step (7) being stirred carries out after 2:1 puddles, put into and lay in the high density polyethylene (HDPE) frame with aseptic polyethylene film, described aseptic polyethylene film is provided with air-vent, and air-vent aperture is 3 millimeters;Above-specified high density polyethylene frame is put into temperature be 20-25 DEG C, air humidity be the thermostatic chamber of 60%, heat 50-60 days.
Embodiment 2, the lily seed bulb scale described in embodiment 1 is bred in seed ball method: comprise the following steps: choosing of (1) lily ball: choose smooth surface, without mouldy, without rotting, girth is at the lily ball of 20cm;(2) ball sterilization is planted: put into by the lily ball chosen in step (1) in broad-spectrum germicide 15 minutes, then being diluted in 3000 times of potassium permanganate diluents 2 minutes being placed in, described broad-spectrum germicide is be diluted to the methyl isophthalic acid H-2-benzimidazole carbamate solution of 1200 times;(3) breaking dormancy: the lily ball after step (2) sterilization is sheltered from heat or light after ventilating 5 hours, put into the freezer that design temperature is-1 DEG C and get final product breaking dormancy in 40 days;(4) dormancy transition: after breaking kind of ball dormancy, temperature of ice house is transferred to 3 DEG C;(5) lily bud scale strips: lily bud scale is peeled off from lily ball foundation portion ecto-entad by the lily ball obtained after step (4), strips 7 layers;(6) rear wound healing is stripped: the lily bud scale after peeling off is put into 5 DEG C, ventilates and promote wound to heal in 6 days;(7) grass carbon is puddled: will be diluted with water to 1000 times of broad-spectrum germicide methyl isophthalic acid H-2-benzimidazole carbamates and be stirred with Pin Shi Top of Denmark turfy soil 1:5000 ratio;(8) epidemic disaster management and control: Pin Shi Top of Denmark turfy soil and the lily bud scale after step (7) being stirred carries out after 2:1 puddles, put into and lay in the high density polyethylene (HDPE) frame with aseptic polyethylene film, described aseptic polyethylene film is provided with air-vent, and air-vent aperture is 3 millimeters;Above-specified high density polyethylene frame is put into temperature be 23 DEG C, air humidity be the thermostatic chamber of 60%, heat 55 days.
Embodiment 3, the lily seed bulb scale described in embodiment 1 or 2 is bred in seed ball method: comprise the following steps: choosing of (1) lily ball: choose smooth surface, without mouldy, without rotting, girth is at the lily ball of 12cm;(2) ball sterilization is planted: put into by the lily ball chosen in step (1) in broad-spectrum germicide 10 minutes, then being diluted in 2000 times of potassium permanganate diluents 1 minute being placed in, described broad-spectrum germicide is be diluted to the methyl isophthalic acid H-2-benzimidazole carbamate solution of 1000 times;(3) breaking dormancy: the lily ball after step (2) sterilization is sheltered from heat or light after ventilating 5 hours, put into the freezer that design temperature is-1 DEG C and get final product breaking dormancy in 40 days;(4) dormancy transition: after breaking kind of ball dormancy, temperature of ice house is transferred to 2 DEG C;(5) lily bud scale strips: lily bud scale is peeled off from lily ball foundation portion ecto-entad by the lily ball obtained after step (4), strips 6 layers;(6) rear wound healing is stripped: the lily bud scale after peeling off is put into 3 DEG C, ventilates and promote wound to heal in 5 days;(7) grass carbon is puddled: will be diluted with water to 500 times of broad-spectrum germicide methyl isophthalic acid H-2-benzimidazole carbamates and be stirred with Pin Shi Top of Denmark turfy soil 1:5000 ratio;(8) epidemic disaster management and control: Pin Shi Top of Denmark turfy soil and the lily bud scale after step (7) being stirred carries out after 2:1 puddles, put into and lay in the high density polyethylene (HDPE) frame with aseptic polyethylene film, described aseptic polyethylene film is provided with air-vent, and air-vent aperture is 3 millimeters;Above-specified high density polyethylene frame is put into temperature be 20 DEG C, air humidity be the thermostatic chamber of 60%, heat 50 days.
Embodiment 4, the lily seed bulb scale described in embodiment 1 or 2 or 3 is bred in seed ball method: comprise the following steps: choosing of (1) lily ball: choose smooth surface, without mouldy, without rotting, girth is at the lily ball of 24cm;(2) ball sterilization is planted: put into by the lily ball chosen in step (1) in broad-spectrum germicide 20 minutes, then being diluted in 4000 times of potassium permanganate diluents 5 minutes being placed in, described broad-spectrum germicide is be diluted to the methyl isophthalic acid H-2-benzimidazole carbamate solution of 1500 times;(3) breaking dormancy: the lily ball after step (2) sterilization is sheltered from heat or light after ventilating 8 hours, put into the freezer that design temperature is-2 DEG C and get final product breaking dormancy in 60 days;(4) dormancy transition: after breaking kind of ball dormancy, temperature of ice house is transferred to 4 DEG C;(5) lily bud scale strips: lily bud scale is peeled off from lily ball foundation portion ecto-entad by the lily ball obtained after step (4), strips 8 layers;(6) rear wound healing is stripped: the lily bud scale after peeling off is put into 6 DEG C, ventilates and promote wound to heal in 7 days;(7) grass carbon is puddled: will be diluted with water to 1500 times of broad-spectrum germicide methyl isophthalic acid H-2-benzimidazole carbamates and be stirred with Pin Shi Top of Denmark turfy soil 1:5000 ratio;(8) epidemic disaster management and control: Pin Shi Top of Denmark turfy soil and the lily bud scale after step (7) being stirred carries out after 2:1 puddles, put into and lay in the high density polyethylene (HDPE) frame with aseptic polyethylene film, described aseptic polyethylene film is provided with air-vent, and air-vent aperture is 3 millimeters;Above-specified high density polyethylene frame is put into temperature be 25 DEG C, air humidity be the thermostatic chamber of 60%, heat 60 days.
Embodiment 5, the lily seed bulb scale described in any one of embodiment 1-4 is bred in seed ball method: the thickness of described aseptic polyethylene film 0.04 millimeter.