CN1057445C - Method for preparing anticancer bioactive peptide preparation - Google Patents

Abstract

The present invention relates to a preparation method for preparing anticancer bioactive peptides, which uses the cell-free extracting solution of human cancer tissue to induce goat spleens to generate and proliferate active peptides, wherein prepared effective components are the cell-free extracting solution. The bioactive peptide preparation has the advantages of no toxic effect, side effect or antigenicity, can manoeuvre and restore the autoimmune function and the anticancer capability of tumour patients, has certain functions of selectively killing and inhibiting the proliferation and the transfer of tumor cells, and inducing the differentiation and the apoptosis of tumor cells and has no interference in normal physiological metabolism.

Description

A kind of preparation method of anticancer bioactive peptide preparation

The present invention relates to a kind of biologically active peptide, particularly its active ingredient is the preparation method of cell-free extract, and its method comprises through animal induces the generation bioactive peptide.

Abroad just have as far back as nineteen twenty-nine and to research and propose spleen and can produce a kind of factor that suppresses tumor growth, and studied the role and influence that development takes place for stomach cancer cell peptide.What relevant both at home and abroad research was adopted all is Placenta Hominis or serum peptide.Do not see reported in literature about animal spleen induced activity peptide and basis and clinical experiment.

The preparation method that the purpose of this invention is to provide a kind of anticancer bioactive peptide can prepare a kind of transfer in this way, recover tumor patient autoimmune function and anti-cancer ability and the preparation that has no side effect.

The objective of the invention is to finish: extract human body cancer tissue earlier by following measure, again itself and normal saline are pulverized in the ratio of 0.5～1g/ml, even oar, multigelation three times, centrifugal treating 15～20 minutes (3000 rev/mins), get the acellular liquid in upper strata goat is carried out multidigit point (back, foot) subcutaneous inducing injected 3～5 times, after injection is last, after 3～5 days the goat arterial blood letting is put extremely, take out Lien Naemorhedi rapidly, liver, thymus is pulverized fast, itself and normal saline are made even oar in 0.5～1g/4ml ratio, put under the cryogenic conditions multigelation three times, more after filtration, centrifugal, ultrafiltration, degerming becomes finished product.

Advantage of the present invention and effect are: preparation method adopts gentle process and comparatively simple, and preparation liquid only contains normal saline, does not contain pungent.Temperature is not had strict demand, need not the special reagent preparation yet.The bioactive peptide that extracts from the animal spleen with specific function is a kind of cell-free extract, has no side effect no antigen, but life-time service.

This preparation had both possessed used clinically cytokines class material at present, killed and wounded the advantage that suppresses tumor cell as interferon, interleukin etc., has again normal cell is not had lethality, strengthened to recover body's immunity; The inducing tumor cell differentiation, the function that enters apoptosis.

Clinical voluntary requirement is accepted to use the back with tens routine late tumor patients of bioactive peptide treatment to be observed, the confirmation bioactive peptide has transfer, recovery patient's autoimmune function and kills and wounds, suppresses the function of tumor cell proliferation, transfer, improve patient's life quality, prolonged patient's vital stage.

This accompanying drawing is the flow chart of this preparation method;

Embodiment: (cancerous tissue: ratio normal saline) is pulverized in 1g/ml with human cancer tissue, even oar multigelation three times, 3000 rev/mins centrifugal 20 minutes, get the acellular liquid in upper strata and goat is carried out multidigit point (back, foot step) is subcutaneous induces injection, each 10 days at interval, induce injection, coinduction 5 times, after the 5th injection, the goat arterial blood letting is put extremely after 3 days.

Relatively under the aseptic condition, take out spleen, liver, thymus rapidly, and pulverize fast, pulverize the back tissue in the (tissue fluid: normal saline) make even oar, put under the cryogenic conditions multigelation three times of 1g/4ml ratio.Freeze thawing liquid filters through multilamellar sterile gauze (more than 20 layers), get filtered solution and carry out ultracentrifugation, 10000 rev/mins 20 minutes.

Learn from else's experience supernatant liquid after centrifugal at room temperature filters through CA-110 dialyser (U.S. Baxter company).

Filtered solution is encapsulated into finished product through 0.2 μ disposable filter degerming.

Claims (1)

1, a kind of preparation method of anticancer bioactive peptide preparation, its method comprises through animal induces the generation bioactive peptide, it is characterized in that extracting earlier human body cancer tissue, again itself and normal saline are pulverized in the ratio of 0.5-1g/ml, even oar, multigelation three times, centrifugal treating 15-20 minute, 3000 rev/mins, get the acellular liquid in upper strata goat is carried out the back, subcutaneous the inducing of foot multidigit point injected 3-5 time, after injection is last, after 3-5 days the goat arterial blood letting is put extremely, take out Lien Naemorhedi rapidly, liver, thymus is pulverized fast, itself and normal saline are made even oar in the 0.5-1g/4ml ratio, multigelation is three times under cryogenic conditions, more after filtration, centrifugal, ultrafiltration, degerming becomes finished product.