CN105728145A - Sample grinder for tissue sample grinding - Google Patents
Sample grinder for tissue sample grinding Download PDFInfo
- Publication number
- CN105728145A CN105728145A CN201610252335.1A CN201610252335A CN105728145A CN 105728145 A CN105728145 A CN 105728145A CN 201610252335 A CN201610252335 A CN 201610252335A CN 105728145 A CN105728145 A CN 105728145A
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- China
- Prior art keywords
- funnel
- plug
- sample
- chassis
- mill
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C19/00—Other disintegrating devices or methods
- B02C19/0056—Other disintegrating devices or methods specially adapted for specific materials not otherwise provided for
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
- G01N2001/2866—Grinding or homogeneising
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- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention belongs to the field of biotechnology instruments, and particularly relates to a sample grinder for plant or animal tissue. The sample grinder is composed of a hanging mill (1), a linkage line (2), a funnel (3) and a funnel plug (4). The hanging mill (1) is connected with a motor through the linkage line (2). A base plate (13) below the hanging mill (1) is matched with the funnel (3) in a threaded manner. Vertical grain (32) is arranged on the outer side of the upper portion of the funnel (3). An inner spherical surface (33) of the funnel (3) is smooth. A funnel opening (34) is arranged at the outer bottom of the funnel (3), and cross grain is arranged on the outer side of the funnel opening (34). The upper surface of the funnel plug (4) is a spherical surface, and spring pieces (42) are arranged on the side face of the funnel plug (4). The funnel plug (4) is buckled and plugged into the funnel opening (34) at the bottom of the funnel (3), and the upper spherical surface (41) of the funnel plug (4) coincides with the inner spherical surface (33) of the funnel (3). According to the sample grinder, sample grinding is thorough, time is short, samples and liquid nitrogen are saved, the probability of cross contamination is lowered, and the risk that a sample pipe explodes is eliminated.
Description
The invention belongs to agricultural and biotech equipment field, particularly to a kind of sample milling machine for plant or animal tissue.
Background technology
Grind away is the single stepping frequently involved in the technical fields such as agricultural, specifically includes: agricultural, biotechnology, pharmacy, gardening etc., and along with the pay attention to day by day that biotechnology is tested by people, grind away test application is more and more extensive.In grind away test, the grind away of tissue is the operation that testing crew one is frequent and requires great effort, such as in large quantities of extractions RNA (ribonucleic acid) or DNA (DNA (deoxyribonucleic acid)) test, it is necessary to each experiment of each time point is repeated tissue grind away.Tissue can easily and fast, the grind away of health be directly connected to test schedule speed and final test effect.
The material of grind away experiment is mainly plant tissue and animal tissue, and period to ensure to organize activity or the integrity of interior molecular substance, so needing to use liquid nitrogen to keep sample to be in low temperature state, therefore in tissue grind away process, test operation is particularly severe.
Tradition tissue grind away operation there is following defect: (1) grind away time length and waste muscle power, general grind away mainly uses mortar, if thoroughly ground, need 20～30min, animal tissue is generally thicker, and the required grind away time can be more of a specified duration, and waste of manpower is also more many；(2) sample waste is serious, it is typically in grind away process to need to be continuously added liquid nitrogen, flies away container adding the fashionable dust that often evokes, additionally also have amount of powder and remain in the inwall of sample milling machine and cannot collect, for measuring less or precious sample, this waste is difficult to accept；(3) liquid nitrogen waste is serious, to be continuously added liquid nitrogen when being typically in grind away, has when there is liquid nitrogen and grind in container, sample can flow everywhere with liquid nitrogen, so now the efficiency of grind away is low, only wait until that liquid nitrogen soon volatilizees most grind away, therefore has substantial amounts of liquid nitrogen and slatterns；(4) grind away is not thorough, often through the color of powder, general grind away judges that whether grind away is complete, but the different times of different tissues or different tissues just cannot effectively directly judge, this problem eliminates often by increasing the grind away time, so so also indirectly causing defect 1), 2) and 3), generally hanging mill sample milling machine is grind away in 1.5ml conical centrifuge tube, reason due to centrifuge tube and rotary head, sample can not thoroughly be ground, sample can not thoroughly crush, and the material needed for may result in follow-up test can not effectively extract；5) there is cross-contamination, owing to grind away is to carry out in the container of opening, so when adding liquid nitrogen, the powder evoked can fly away everywhere, often through liquid nitrogen and capsule contamination to other test specimen, for requiring the strict test of comparison, such operation may result in test error；6) sample adds, with directly contacting of liquid nitrogen, the risk that sample stores, often with part liquid nitrogen when sample is stored into centrifuge tube, when temperature is increased to a certain degree, liquid nitrogen in centrifuge tube can be changed into gas, the pressure in centrifuge tube can be made to increase, so the risk that centrifuge tube explodes often occurs in follow-up test.
Summary of the invention
The invention aims to solve common sample milling machine grind away time length, waste muscle power, waste sample and liquid nitrogen, grind away not thoroughly and the problem of cross-contamination, it is provided that a kind of easy and simple to handle, save time, the tissue sample milling machine of health.It is characterized in that, tissue sample milling machine is by hanging mill (1), train line (2), funnel (3) funnel plug (4)；Hang mill (1) to be connected with motor by one section of train line 1.5 meters long (2).
The described handle place hanging mill (1) is provided with the groove (11) preventing hands from sliding, hang mill (1) and be arranged below chassis (13), chassis (13) is reinforced by reinforcement (12) with hanging mill (1), and the outside of chassis (13) is provided with external screw thread (14)；Rotary head (15) is stretched out from the bottom center of chassis (13).
The upper inner of described funnel (3) is provided with female thread (31), and described chassis (13) are matched with the female thread (31) of funnel (3) by external screw thread (14).
The upper outside of described funnel (3) is provided with the vertical line (32) conveniently screwed, the inner surface (33) of funnel (3) is smooth, it is hemispherical, the outer bottom of funnel (3) is provided with bell mouth (34), and the outside of bell mouth (34) is provided with is easy to the band that funnel plug (4) is fixing.
The upper surface of described funnel plug (4) is sphere, the side of funnel plug (4) is provided with spring leaf (42), when funnel plug (4) fastens bell mouth (34) filling in funnel (3) bottom, the upper sphere of funnel plug (4) overlaps with the inner surface (33) of funnel (3).
The thickness on described chassis (13) is 5～10mm, its diameter 20～50mm.
Internal diameter 4～the 9mm of described bell mouth (34)；Match with the inner cylinder face of bell mouth (34) in the face of cylinder of funnel plug (4).
Described funnel 3 is metal stainless steel material
The feature of the present invention and having the beneficial effect that
(1) using electric mill to grind, the grind away time shortens, it is to avoid traditional grind away is lost time defect with manpower；(2) hand-held grind away is easy to operate, quick, safety, at least can improve the work efficiency 80% of testing crew；(3) unique leaf that flies designs, and drives when grind away sample powder to circulate with air, makes sample fully be smashed, and eliminates common grind away probability not thoroughly；(4) confined space during grind away, the cross-contamination occurred when reducing common grind away, it is ensured that follow-up test result is more accurate, and confined space reduces the loss that sample flies away simultaneously；(5) mortar inner wall smooth, it is to avoid common grind away rear section sample is not easy the defect collected；(6) can directly put in liquid nitrogen with mortar interval in handle lower end, conduction hemisphere can ensure that and is in low temperature state inside mortar, this kind of grind away mode reduces the waste of liquid nitrogen, and isolation liquid nitrogen contacts with the direct of sample simultaneously, eliminates the danger of centrifuge tube blast；(7) grind awaies can repeatedly use, avoid and common hang the defect that grind away of grinding machine is used only once, it is ensured that being smoothed out of test.
Accompanying drawing explanation
Fig. 1 is the main assembling schematic diagram of tissue sample milling machine；
Fig. 2 is the bottom assembling schematic diagram of tissue sample milling machine；
Fig. 3 is sample collection schematic diagram；
In figure, 1--grinding machine, 2--train line, 3--funnel, 4--funnel plug, 11--groove, 12--reinforcement, 13--chassis, 14--external screw thread, 15--rotary head, 31--female thread, 32--vertical line, 33--Internal Spherical Surface, 34--bell mouth, 4--funnel plug, the upper sphere of 41--, 42--spring leaf.
Detailed description of the invention
Below in conjunction with drawings and Examples, the concrete structure of the present invention is further illustrated.
The main assembling structure of tissue sample milling machine is as shown in Figure 1, it is characterised in that, tissue sample milling machine is by hanging mill (1), train line (2), funnel (3) funnel plug (4)；Hang mill (1) to be connected with motor by one section of train line 1.5 meters long (2).
The bottom assembling structure of tissue sample milling machine is as shown in Figure 2, the described handle place hanging mill (1) is provided with the groove (11) preventing hands from sliding, hang mill (1) and be arranged below chassis (13), chassis (13) is reinforced by reinforcement (12) with hanging mill (1), and the outside of chassis (13) is provided with external screw thread (14)；Rotary head (15) is stretched out from the bottom center of chassis (13)；The upper inner of described funnel (3) is provided with female thread (31), and described chassis (13) are matched with the female thread (31) of funnel (3) by external screw thread (14)；The upper outside of described funnel (3) is provided with the vertical line (32) conveniently screwed, the inner surface (33) of funnel (3) is smooth, it is hemispherical, the outer bottom of funnel (3) is provided with bell mouth (34), and the outside of bell mouth (34) is provided with is easy to the band that funnel plug (4) is fixing；The upper surface of described funnel plug (4) is sphere, the side of funnel plug (4) is provided with spring leaf (42), when funnel plug (4) fastens bell mouth (34) filling in funnel (3) bottom, the upper sphere of funnel plug (4) overlaps with the inner surface (33) of funnel (3)；The thickness on described chassis (13) is 5～10mm, its diameter 20～50mm；Internal diameter 4～the 9mm of described bell mouth (34)；Match with the inner cylinder face of bell mouth (34) in the face of cylinder of funnel plug (4)；Described funnel (3) is metal stainless steel material.
Use funnel plug (4) to fill in the bell mouth (34) of funnel (3) bottom, the sample good through liquid nitrogen freezing is put into the funnel (3) also passing through liquid nitrogen freezing and crossing；Being screwed by funnel (3) is connected on chassis (13), and power-on, sample is constantly smashed in rotary head (15).
Sample collection is as shown in Figure 3, after sample grind away, sample powder is swept to gently the side of funnel (3), take off funnel plug (4), bell mouth (34) is inserted into liquid nitrogen freezing good centrifuge tube (5) mouth, collects sample.Afterwards funnel (3) is partly screwed on chassis (13), take off funnel plug (4), tissue grinder is placed in ethanol, start power supply and clean dismembyator, take out tissue grinder afterwards, start power supply and dried or paper using wiped clean is used for testing next time.
The above; being only the present invention preferably detailed description of the invention, but protection scope of the present invention is not limited thereto, any those familiar with the art is in the technical scope that the invention discloses; the change that can readily occur in or replacement, all should be encompassed within protection scope of the present invention.Therefore, protection scope of the present invention should be as the criterion with scope of the claims.
Claims (3)
1. the sample milling machine being used for organizing grind away, it is characterised in that by hanging mill (1), train line (2), funnel (3) funnel plug (4)；Hang mill (1) to be connected with motor by one section of train line 1.5 meters long (2)；
The described handle place hanging mill (1) is provided with the groove (11) preventing hands from sliding, hang mill (1) and be arranged below chassis (13), chassis (13) is reinforced by reinforcement (12) with hanging mill (1), and the outside of chassis (13) is provided with external screw thread (14)；Rotary head (15) is stretched out from the bottom center of chassis (13)；The upper inner of described funnel (3) is provided with female thread (31), and described chassis (13) are matched with the female thread (31) of funnel (3) by external screw thread (14).
The upper outside of funnel (3) is provided with the vertical line (32) conveniently screwed, the Internal Spherical Surface (33) of funnel (3) is smooth, it is hemispherical, the outer bottom of funnel (3) is provided with bell mouth (34), and the outside of bell mouth (34) is provided with is easy to the band that funnel plug (4) is fixing；The upper surface of described funnel plug (4) is sphere, the side of funnel plug (4) is provided with spring leaf (42), when funnel plug (4) fastens bell mouth (34) filling in funnel (3) bottom, the upper sphere 41 of funnel plug (4) overlaps with the Internal Spherical Surface (33) of funnel (3).
2. according to claim 1 a kind of for organizing the sample milling machine of grind away, it is characterised in that the thickness of chassis (13) is 5～10mm, its diameter 20～50mm.
3. according to claim 1 a kind of for organizing the sample milling machine of grind away, it is characterised in that the internal diameter 4～9mm of bell mouth (34)；Match with the inner cylinder face of bell mouth (34) in the face of cylinder of funnel plug (4).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610252335.1A CN105728145B (en) | 2016-04-21 | 2016-04-21 | A kind of sample milling machine for tissue grind away |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610252335.1A CN105728145B (en) | 2016-04-21 | 2016-04-21 | A kind of sample milling machine for tissue grind away |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105728145A true CN105728145A (en) | 2016-07-06 |
| CN105728145B CN105728145B (en) | 2018-01-26 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610252335.1A Active CN105728145B (en) | 2016-04-21 | 2016-04-21 | A kind of sample milling machine for tissue grind away |
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| Country | Link |
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| CN (1) | CN105728145B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109855932A (en) * | 2019-01-02 | 2019-06-07 | 中铝材料应用研究院有限公司 | A method of preparing rolling aluminium alloy sheet EBSD sample |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108855504B (en) * | 2018-06-11 | 2021-08-27 | 瑞安市华澳绿康农业科技有限公司 | Sample grinding device for tissue polishing in laboratory |
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| US4072275A (en) * | 1975-12-05 | 1978-02-07 | Mettler Instruments Ag | Sample preparation device |
| JPS63115438U (en) * | 1987-01-19 | 1988-07-25 | ||
| CN203658130U (en) * | 2014-01-13 | 2014-06-18 | 中国矿业大学 | Device for simplifying process for determining plant chlorophyll |
| CN204286891U (en) * | 2014-11-24 | 2015-04-22 | 中国烟草总公司郑州烟草研究院 | One grows tobacco hard tissues cryogrinding device |
| CN204359699U (en) * | 2015-01-27 | 2015-05-27 | 于艳丽 | A kind of instrument for detecting pesticide residue through |
| CN204944925U (en) * | 2015-09-17 | 2016-01-06 | 四川农业大学 | A kind of refrigeration grinding machine |
| CN105251564A (en) * | 2015-11-19 | 2016-01-20 | 江西师范大学 | Biological sample freezing and grinding machine |
| CN205599281U (en) * | 2016-04-21 | 2016-09-28 | 广西作物遗传改良生物技术重点开放实验室 | A grind appearance ware for organizing grind appearance |
-
2016
- 2016-04-21 CN CN201610252335.1A patent/CN105728145B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1289121A (en) * | 1969-02-10 | 1972-09-13 | ||
| DE2260277A1 (en) * | 1972-11-17 | 1974-06-12 | Saiki | LAB ANALYSIS INSTRUMENTS |
| US4072275A (en) * | 1975-12-05 | 1978-02-07 | Mettler Instruments Ag | Sample preparation device |
| JPS63115438U (en) * | 1987-01-19 | 1988-07-25 | ||
| CN203658130U (en) * | 2014-01-13 | 2014-06-18 | 中国矿业大学 | Device for simplifying process for determining plant chlorophyll |
| CN204286891U (en) * | 2014-11-24 | 2015-04-22 | 中国烟草总公司郑州烟草研究院 | One grows tobacco hard tissues cryogrinding device |
| CN204359699U (en) * | 2015-01-27 | 2015-05-27 | 于艳丽 | A kind of instrument for detecting pesticide residue through |
| CN204944925U (en) * | 2015-09-17 | 2016-01-06 | 四川农业大学 | A kind of refrigeration grinding machine |
| CN105251564A (en) * | 2015-11-19 | 2016-01-20 | 江西师范大学 | Biological sample freezing and grinding machine |
| CN205599281U (en) * | 2016-04-21 | 2016-09-28 | 广西作物遗传改良生物技术重点开放实验室 | A grind appearance ware for organizing grind appearance |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109855932A (en) * | 2019-01-02 | 2019-06-07 | 中铝材料应用研究院有限公司 | A method of preparing rolling aluminium alloy sheet EBSD sample |
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| Publication number | Publication date |
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| CN105728145B (en) | 2018-01-26 |
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Inventor after: Qu Junjie Inventor after: Yin Ling Inventor after: Huang Yu Inventor after: Lu Jiang Inventor after: Wei Yuanwen Inventor after: Guo Wenfeng Inventor after: Fang Hui Inventor before: Yin Ling Inventor before: Qu Junjie Inventor before: Huang Yu Inventor before: Lu Jiang Inventor before: Wei Yuanwen Inventor before: Guo Wenfeng Inventor before: Fang Hui |
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