CN105713827B - A kind of nitrifying bacteria enrichment culture apparatus and cultural method - Google Patents
A kind of nitrifying bacteria enrichment culture apparatus and cultural method Download PDFInfo
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Abstract
The invention discloses a kind of nitrifying bacteria enrichment culture apparatus, including outer barrel reactor and ceramic membrane inner cylinder, outer barrel reactor is opened type structure, and inside sets the ceramic membrane inner cylinder made of ceramic diaphragm plate；Wall and bottom set aerating system outer tube, and aerating system and sludge are located at ceramic membrane inner cylinder both sides respectively, and during aeration, sludge is cultivated in ceramic membrane inner cylinder, and gas enters inner cylinder by ceramic fenestra and realizes oxygen supply；External reactors bottom is provided with discharge outlet, is connected by vavuum pump with the header tank sealed, after culture terminates, starts vavuum pump and water is drained into closed header tank, sludge is by ceramic membrane interception.Nitrifying bacteria enrichment cultural method of the present invention is carried out using above-mentioned culture apparatus.The present invention cultivates unit by setting ceramic membrane inner cylinder to be used as nitrifier in reactor, effectively reduces sludge settling and changes the water time, shortens cultivation cycle, and can prevent that sludge flco is lost in during draining, ensure that the steady growth of sludge.
Description
Technical field
The invention belongs to environmental microorganism and technical field of sewage, and in particular to a kind of nitrifying bacteria enrichment culture apparatus
And cultural method.
Background technology
To prevent the generation in lake and other receiving water body eutrophication, each municipal sewage plant applies new operation
Method and control strategy carry out denitrogenation dephosphorizing.Obtained with the intervention of new microbiological treatment technology, the effect of sewage treatment facility
To significantly improving.When nitration denitrification technology is applied to processing sewage containing ammonia, be economically and technically respectively provided with it is higher can
Row.
In biological denitrification method, either traditional nitrification-denitrification, or new short-cut nitrification-denitrification and short
Journey nitration-anaerobic ammoxidation is all needed by nitrification removal of ammonia and nitrogen.Nitrifier containing good biological activity in activated sludge
It is the premise and critical support of biological denitrificaion, therefore, culture and the enrichment of nitrifier are increasingly paid attention to by researcher.But
Nitrobacter is slower in autotrophic type microorganism, reproduction speed.Found in the enrichment culture process to nitrifier, sludge flco is in
Suspended state is grown in nutrient solution, and the flco scale of construction is small, and settling velocity is slower, causes the cultivation cycle of nitrifier especially long.
During batch cultivation is carried out, it is dense that prior art typically improves influent ammonia nitrogen by the way of directly supplement high concentration nitrogen source
Degree, but high concentration product accumulation so is will result in, growth and the action activity of nitrifier can be suppressed.If take every batch
All modes of draining, then the sedimentation time grown being needed very much, total cultivation cycle is longer, cumbersome, and due to being that nature sinks
Drop, would generally contain a certain amount of sludge suspension thing in clear water after isolation, this, which not only results in effluent quality and not met, wants
Ask, and the loss of sludge can also influence the efficiency of culturing sludge.
CN20120014634.3 discloses a kind of method and apparatus of fast enriching ammonia oxidation bacteria.Mainly use batch-type
Activated sludge process, it is enriched with by gradually stepping up ammonia nitrogen concentration and the method for pH process control in nutrient solution.The cultural method
Including intaking, plus alkali, aeration, plus alkali, aeration, backflow, precipitation, draining and leave unused etc. step, each step was by may be programmed
Range controller is controlled.The method and device use natural subsidence in the sludge settling stage, time-consuming longer, cause total culture
Cycle is longer.
CN201310277522.1 discloses a kind of device and method cultivated and waste water is handled using aerobic particle mud.
Supporting for granule sludge in processing system can be achieved by the control to process conditions in water inlet and processing procedure in the device and method
Removed into while maintaining, and organic and nutrition pollutant is realized using its excellent performance.But this device is from bottom
Water inlet, top draining, middle part spoil disposal.Although four steps in the existing SBR process operations cycle can be reduced to three, realize
Processing system is intake and water outlet is carried out simultaneously, but needs when intake from bottom control to intake flow velocity and flow, otherwise Sludge Bed
The state excessively fluidized occurs in layer.Specifically, the turbulence of Sludge Bed and the height of Mixed Zone caused by waste water are injected
Less than the 50% of total bed height, the 25% of preferably smaller than total bed height, the 15% of more preferably less than total bed height.Such one
Come, flooding time is also longer, and total cycle time is still longer.
The content of the invention
In view of the shortcomings of the prior art, the invention provides a kind of nitrifying bacteria enrichment culture apparatus and cultural method.This hair
Bright device in reactor by setting ceramic membrane inner cylinder be used as nitrifier culture unit, when effectively reducing sludge settling and changing water
Between, cultivation cycle is shortened, and can prevent that sludge flco is lost in during draining, ensure that the steady growth of sludge.
Nitrifying bacteria enrichment culture apparatus of the present invention, including outer barrel reactor and ceramic membrane inner cylinder, outer barrel reactor are opening
Formula structure, inside set the ceramic membrane inner cylinder made of ceramic diaphragm plate；Aerating system is set in ceramic membrane inner tank theca and bottom, is exposed
Gas system and sludge are located at ceramic membrane inner cylinder both sides respectively, and during aeration, sludge is cultivated in ceramic membrane inner cylinder, and gas passes through
Ceramic fenestra enters inner cylinder and realizes oxygen supply；External reactors bottom is provided with discharge outlet, passes through vavuum pump and the header tank phase of sealing
Even, after culture terminates, start vavuum pump and water is drained into closed header tank, sludge is then by ceramic membrane interception in inner cylinder.
In the present invention, the geometry of ceramic membrane inner cylinder can be identical or different with outer barrel reactor, it is preferred to use identical
Geometry.The volume of ceramic membrane inner cylinder is 0.5~0.8 times of outer barrel reactor volume.The wall thickness of ceramic membrane inner cylinder be 1~
5cm, film hole diameter are 0.05~0.5 μm.Ceramic membrane inner cylinder is concordant with external reactors top and is tightly connected.
In the present invention, sludge is cultivated in ceramic membrane inner cylinder, after culture terminates, is started vacuum pump drainage, is passed through
Suction filtration effect drains into water closed header tank, and sludge is then by ceramic membrane interception in inner cylinder.Outer barrel reactor is fixed with into water
Pipe, bottom is provided with discharge outlet.Ceramic membrane inner cylinder bottom is provided with sludge-discharge pipe, passes through the outer row of Valve controlling sludge.
In the present invention, aerating system is arranged at the outer surface of ceramic membrane inner cylinder, is arranged in inner cylinder bottom in matts shape,
Arranged on surrounding outer wall in horizontal bar；Aerating system and sludge are located at the both ends of ceramic diaphragm plate respectively, and during aeration, gas passes through pottery
Porcelain fenestra realizes oxygen supply.The method for arranging of this aerating system can realize the backwash to ceramic diaphragm plate, effectively prevent ceramics
The blocking of diaphragm plate, ensure rational infiltration flux.
The present invention is comprised the following steps using the nitrifying bacteria enrichment cultural method of above-mentioned culture apparatus：
（1）Nitrifier activated sludge is added in the ceramic membrane inner cylinder of culture apparatus；
（2）Nutrient solution is injected into ceramic membrane inner cylinder so that influent ammonium concentration is 50~150mg/L, starts aeration system
System aeration；
（3）When ammonia nitrogen concentration is less than 20mg/L, stop aeration, start vacuum pump drainage；Fresh cultured is supplemented after draining
Liquid, the mode for improving influent ammonium concentration step by step is taken to carry out batch cultivation so that final ammonia nitrogen concentration improved to 500~
600mg/L；
（4）When ammonia nitrogen removal frank is up to more than 90%, stops aeration, vacuum drainage, collect nitrifying sludge.
Step of the present invention（1）According to the volume of ceramic membrane inner cylinder, control the initial sludge concentration of cultivating system for 200~
500mg/L, preferably 200~300mg/L.
Step of the present invention（2）Start aerating system to be aerated, control the dissolved oxygen concentration in ceramic membrane inner cylinder 1.0~
1.5mg/L, temperature are 30~35 DEG C, and pH value is 7.0~9.0.Described nutrient solution is autogamy nitrogen-containing wastewater or production life
The good nitrogen-containing wastewater of caused various biodegradabilities in work, B/C ratios are not higher than 500mg/L more than 0.4, COD concentration.Cultivated
Cheng Zhong, can be supplemented with the growth promoter beneficial to nitrobacteria growth, and the growth promoter includes metal salt and more amines
Matter, wherein metal salt are 40~100 parts by weight, preferably 50~80 parts by weight, and polyamines are 5~30 parts by weight, preferably
For 10~20 parts by weight；Described metal salt is calcium salt, magnesium salts, molysite and mantoquita, Ca2+、Mg2+、Fe2+And Cu2+Mol ratio be
（5~15）:（5~25）:（1~8）:（0.5~5）, it is preferably（8~12）:（10~20）:(2~6):（1~4）.The accelerator
For that in the incubation of nitrobacteria, can enable nitrobacteria, fast-growth is bred in a short time, the nitrification cultivated
Bacterial activity is high, tolerance impact capacity is strong.
Step of the present invention（3）After stopping aeration, the discharge outlet and vavuum pump of device for opening bottom, start vacuum pump drainage,
The gauge pressure for adjusting vavuum pump is 0.1~0.2MPa, with the gradual increase of ceramic membrane inner cylinder both sides pressure difference, cultivates the water in unit
Header tank gradually is flowed out to, when water all oozes out, closes vavuum pump and draining valve.Start aerating system after draining, mend simultaneously
Fresh medium is filled, takes the mode for improving influent ammonium concentration step by step to carry out batch cultivation, each ammonia nitrogen concentration increase rate
For 50~100mg/L.
Step of the present invention（4）When ammonia nitrogen removal frank is up to more than 90%, stop aeration, start vacuum pump drainage, adjust vacuum
The gauge pressure of pump is 0.1~0.2MPa.The valve of sludge-discharge pipe is opened when collecting nitrifying sludge, by cultured sludge whole or portion
Divide discharge, afterwards repeat step（1）Extremely（4）Carry out next batch enrichment culture.
In the present invention, because nitrifying process is acid process, when pH value is less than setting value in incubation, pass through measuring pump
Alkali lye is supplemented to cultivating system, alkali lye is saturated solution of sodium bicarbonate, when pH value reaches setting value, is stopped into alkali.
Compared with prior art, the invention has the advantages that：
1st, present invention culturing sludge unit made of setting ceramic membrane materials inside culture apparatus, using what is vacuumized
Mode carries out draining, not only eliminates the water decanter that conventional apparatus uses, and effectively reduce sludge during culturing sludge
The time of water is changed in sedimentation, significantly shorten the culturing sludge cycle.The present invention can be quick under the driving of pressure using ceramic membrane
The characteristics of carrying out fluid separation, the sludge flco not settled is firmly pinned, ensure that the clarity of water outlet；And due to ceramics
The good filtering of film, purification properties, the loss of sludge flco can be effectively prevented, ensure that the steady growth of sludge.
2nd, aerating system is evenly arranged in ceramic membrane inner cylinder surrounding and bottom outer wall, realizes sludge and the abundant of water body mixes
Close, not only acted as stirring action, additionally it is possible to meet the dissolved oxygen condition needed for thalline culture, and this aerator is arranged in
The outside of unit is cultivated, the backwash to ceramic diaphragm plate is realized in aeration process, effectively prevent sludge flco to ceramic membrane
The blocking of plate, it ensure that the optimal infiltration flux of ceramic diaphragm plate.
3rd, the present invention add growth promoter in nitrifying bacteria enrichment incubation, contribute to nitrifier fast-growth and
Granulating, further increases the settling property of nitrifier, and outlet effect is more preferable.
4th, the culture of nitrifier is carried out using apparatus of the present invention, cultural method simple and flexible, cultivation cycle is short, processing cost
Low, culture efficiency is high, has good economic benefit and environmental benefit.
Brief description of the drawings
Fig. 1 is the three-dimensional structure diagram of nitrifier culture apparatus of the present invention；
The outer barrel reactors of wherein 1-, 2- ceramic membrane inner cylinders, 3- aerating systems, 4- sludge-discharge pipes, 5- drainpipes.
Fig. 2 is the top view of culture apparatus of the present invention.
Fig. 3 is the layout structure figure of aerating system of the present invention；
Wherein 6- total inlet pipes, 7- aeration tubes, 8- aeration heads, tube end plate in 9- ceramic membranes, 10- ceramic membrane inner cylinder side plates.
Embodiment
The present invention is described in further detail with reference to the accompanying drawings and examples.
Fig. 1 and Fig. 2 show nitrifying bacteria enrichment culture apparatus of the present invention, including outer barrel reactor 1 and ceramic membrane inner cylinder 2,
Outer barrel reactor 1 is opened type structure, and length is 1.2m；Inside sets the ceramic membrane inner cylinder made of ceramic diaphragm plate,
Length and width is 1m, a height of 0.8m.The dischargeable capacity of ceramic membrane inner cylinder is 700L.Ceramic membrane 1~5cm of thickness of slab, film hole diameter 0.05
~0.5 μm.Ceramic membrane inner cylinder is concordant with external reactors top and is tightly connected.
Aerating system 3 is set in the outer surface of ceramic membrane inner cylinder, aerating system and sludge are located at ceramic membrane inner cylinder two respectively
Side, during aeration, sludge is cultivated in ceramic membrane inner cylinder, and gas enters inner cylinder by ceramic fenestra and realizes oxygen supply.Aeration system
The concrete structure of system is as shown in figure 3, including total inlet pipe 6, aeration tube 7, aeration head 8, and aeration tube and aeration head are in ceramic membrane
The outer surface of tube end plate 9 is arranged in matts shape, is arranged in the outer surface of ceramic membrane inner cylinder side plate 10 in horizontal bar.This aerating system
Method for arranging can realize backwash to ceramic diaphragm plate, effectively prevent the blocking of ceramic diaphragm plate, ensure that rational infiltration is logical
Amount.
Drainpipe 5 is provided with outer barrel reactor bottom, is connected by vavuum pump with the header tank sealed, when culture terminates
Afterwards, start vavuum pump and water is drained into closed header tank, sludge is then by ceramic membrane interception in inner cylinder.Ceramic membrane inner cylinder bottom is set
Sludge-discharge pipe 4 is equipped with, passes through the outer row of Valve controlling sludge.
It is as follows using the nitrifying bacteria enrichment cultural method of above-mentioned culture apparatus, specific operation process：
（1）Nitrifier activated sludge is added in the ceramic membrane inner cylinder 2 of culture apparatus first, according to the body of ceramic membrane inner cylinder
Product, the initial sludge concentration for controlling cultivating system is 200~500mg/L；
（2）Nutrient solution is injected into ceramic membrane inner cylinder, nutrient solution is to be produced in autogamy nitrogen-containing wastewater or production and living
The good nitrogen-containing wastewater of various biodegradabilities, it is 50~150mg/L to control influent ammonium concentration.Start aerating system 3 to carry out
Aeration, the dissolved oxygen concentration in ceramic membrane inner cylinder is controlled in 1.0~1.5mg/L, temperature is 30~35 DEG C, pH value is 7.0~
9.0；
（3）When ammonia nitrogen concentration is less than 20mg/L, stop aeration, start vacuum pump drainage, the gauge pressure for adjusting vavuum pump is
0.1~0.2MPa.Fresh medium is supplemented after draining, takes the mode for improving influent ammonium concentration step by step to carry out batch cultivation,
So that final ammonia nitrogen concentration is improved to 500~600mg/L, each ammonia nitrogen concentration increase rate is 50~100mg/L；
（4）When ammonia nitrogen removal frank is up to more than 90%, stop aeration, start vacuum pump drainage, the gauge pressure for adjusting vavuum pump is
0.1~0.2MPa, collect cultured nitrifying sludge.Repeat step afterwards（1）Extremely（4）Carry out next batch enrichment culture.
Embodiment 1
Using the culture apparatus enrichment culture nitrifier shown in Fig. 1, wherein ceramic membrane thickness of slab 2cm, film hole diameter is 0.1 μ
m.It is that 200mg/L adds nitrifier activated sludge in ceramic membrane inner cylinder according to initial sludge concentration, while adds ammonia nitrogen concentration
For 50mg/L nutrient solution 500mL, start aerating system and be aerated.In incubation, maintain the dissolved oxygen of cultivating system dense
Degree is in 1.0~1.5mg/L, and temperature is at 32 DEG C, pH value 7.8.Because nitrifying process is acid process, pH value is low in incubation
When setting value, alkali lye is supplemented to cultivating system by measuring pump, alkali lye is saturated solution of sodium bicarbonate, when pH value reaches setting
During value, stop into alkali.
Ammonia nitrogen concentration is less than 20mg/L after culture 4h, stops aeration.The gauge pressure for adjusting vavuum pump is 0.1MPa, starts vacuum
Pump carries out draining, treats that water is all discharged, closes vavuum pump and draining valve, drainage procedure about 10min, go out clarification of water, suspension pole
It is few.
During second of water inlet, the nutrient solution 500L that ammonia nitrogen concentration is 150mg/L is added.Ammonia nitrogen concentration is less than 20mg/ after 10h
L.Stop aeration, start vavuum pump and carry out draining.
When third time is intake, the nutrient solution 500L that ammonia nitrogen concentration is 250mg/L, after this time cultivating 16h, ammonia nitrogen concentration are added
Less than 20mg/L.Stop aeration, start vavuum pump and carry out draining.
During the 4th water inlet, the nutrient solution 500L that ammonia nitrogen concentration is 350mg/L, after this time cultivating 23h, ammonia nitrogen concentration are added
Less than 20mg/L.Stop aeration, start vavuum pump and carry out draining.
During the 5th water inlet, the nutrient solution 500L that ammonia nitrogen concentration is 450mg/L, after this time cultivating 30h, ammonia nitrogen concentration are added
Less than 20mg/L.Stop aeration, start vavuum pump and carry out draining.
During the 6th water inlet, the nutrient solution 500L that ammonia nitrogen concentration is 550mg/L, after this time cultivating 39h, ammonia nitrogen concentration are added
Less than 20mg/L.The sludge concentration now cultivated in unit is 2000mg/L.Stop aeration, the gauge pressure for adjusting vavuum pump is
0.1MPa, start vavuum pump and carry out draining.Then the valve of sludge-discharge pipe is opened, cultured sludge is completely or partially arranged
Go out, so far first culturing sludge terminates.Calculate total incubation time：4h+10h+16h+23h+30h+39h=122h, each draining
Time about 10min, six total water discharge time about 1h, therefore cultivate a collection of sludge and always take time about 123h.
Embodiment 2
Using the culture apparatus enrichment culture nitrifier shown in Fig. 1, wherein ceramic membrane thickness of slab 3cm, film hole diameter is 0.25 μ
m.It is that 300mg/L adds nitrifier activated sludge in ceramic membrane inner cylinder according to initial sludge concentration, while adds ammonia nitrogen concentration
For 100mg/L nutrient solution 500mL, start aerating system and be aerated.In incubation, maintain the dissolved oxygen of cultivating system dense
Degree is in 1.0~1.5mg/L, and temperature is at 35 DEG C, pH value 8.5.Because nitrifying process is acid process, pH value is low in incubation
When setting value, alkali lye is supplemented to cultivating system by measuring pump, alkali lye is saturated solution of sodium bicarbonate, when pH value reaches setting
During value, stop into alkali.
Ammonia nitrogen concentration is less than 20mg/L after culture 6h, stops aeration.The gauge pressure for adjusting vavuum pump is 0.1MPa, starts vacuum
Pump carries out draining, treats that water is all discharged, closes vavuum pump and draining valve, drainage procedure about 6min, go out clarification of water, suspension pole
It is few.Then take the mode for improving influent ammonium concentration step by step to carry out batch cultivation so that final ammonia nitrogen concentration improve to
600mg/L, each ammonia nitrogen concentration increase rate are 100mg/L.
Enter for 6th time after Aquaponic terminates, the sludge concentration now cultivated in unit is 2200mg/L.Stop aeration, regulation
The gauge pressure of vavuum pump is 0.1MPa, starts vavuum pump and carries out draining.Then the valve in sludge-discharge pipe road is opened, by cultured dirt
Mud is completely or partially discharged, and so far first culturing sludge terminates.Always incubation time is：6h+11h+18h+25h+33h+41h=
134h, each water discharge time about 6min, six total water discharge time about 36min, therefore cultivate a collection of sludge and always take time about 135h.
Embodiment 3
Using the culture apparatus enrichment culture nitrifier shown in Fig. 1, wherein ceramic membrane thickness of slab 5cm, film hole diameter is 0.5 μ
m.It is that 500mg/L adds nitrifier activated sludge in ceramic membrane inner cylinder according to initial sludge concentration, while adds ammonia nitrogen concentration
For 150mg/L nutrient solution 500mL, start aerating system and be aerated.In incubation, maintain the dissolved oxygen of cultivating system dense
Degree is in 1.0~1.5mg/L, and temperature is at 30 DEG C, pH value 7.5.Because nitrifying process is acid process, pH value is low in incubation
When setting value, alkali lye is supplemented to cultivating system by measuring pump, alkali lye is saturated solution of sodium bicarbonate, when pH value reaches setting
During value, stop into alkali.
Ammonia nitrogen concentration is less than 20mg/L after culture 4h, stops aeration.The gauge pressure for adjusting vavuum pump is 0.1MPa, starts vacuum
Pump carries out draining, treats that water is all discharged, closes vavuum pump and draining valve, drainage procedure about 5min, go out clarification of water.Then take by
The mode that level improves influent ammonium concentration carries out batch cultivation, and final ammonia nitrogen concentration is improved to 550mg/L, and each ammonia nitrogen concentration carries
High-amplitude is 100mg/L.
Enter for 5th time after Aquaponic terminates, the sludge concentration now cultivated in unit is 1800mg/L.Stop aeration, regulation
The gauge pressure of vavuum pump is 0.1MPa, starts vavuum pump and carries out draining.Then the valve in sludge-discharge pipe road is opened, by cultured dirt
Mud is completely or partially discharged, and so far first culturing sludge terminates.Always incubation time is：4h+9h+13h+25h+33h=84h, often
Secondary water discharge time about 5min, six total water discharge time about 25min, therefore cultivate a collection of sludge and always take time about 84.5h.
Embodiment 4
Handling process and operating condition are adding nitrobacteria growth promotion with embodiment 1, difference in incubation
Agent, the growth promoter include metal salt and polyamines, and wherein metal salt is 50 parts by weight, and polyamines are essence
Amine, 20 parts by weight, metal salt are calcium sulfate, magnesium sulfate, ferrous sulfate and copper sulphate, wherein Ca2+、Mg2+、Fe2+And Cu2+Rub
You are than being 9:15:4:2.
Enter for 6th time after Aquaponic terminates, the sludge concentration cultivated in unit is 2200mg/L.Calculate total incubation time：3h
+ 9h+14h+20h+25h+33h=104h, each water discharge time about 10min, six total water discharge time about 1h, therefore cultivate a collection of
Sludge always takes time about 105h.
Embodiment 5
Handling process and operating condition are adding the life of nitrobacteria growth with embodiment 2, difference in incubation
Long accelerator, the growth promoter include metal salt and polyamines, and wherein metal salt is 80 parts by weight, polyamines
For spermidine, 10 parts by weight, metal salt is calcium sulfate, magnesium sulfate, ferrous sulfate and copper sulphate, wherein Ca2+、Mg2+、Fe2+With
Cu2+Mol ratio be 9:15:4:2.
Enter for 6th time after Aquaponic terminates, the sludge concentration cultivated in unit is 2400mg/L.Calculating total incubation time is：
5h+10h+16h+22h+29h+35h=117h, each water discharge time about 11min, six total water discharge time about 36min, therefore train
A collection of sludge is supported always to take time about 118h.
Comparative example 1
Handling process and operating condition are with embodiment 1, and difference is to use conventional culturing sludge device, without true
Idle discharge water, but draining after natural subsidence.After culture terminates, the sludge concentration cultivated in unit is 1700mg/L.Calculate total training
The foster time is：4h+10h+17h+24h+31h+40h=126h, each water discharge time about 5h, six total water discharge time about 30h, because
This cultivates a collection of sludge and always taken time about 156h.This is due to that each draining is not thorough, a certain amount of nitration product of meeting remaining, with
The increase of water inlet number, influences the efficient removal of ammonia nitrogen and the steady growth of sludge, causes cultivation cycle to extend, and water outlet
It is not limpid enough.
Comparative example 2
With embodiment 1, difference is using filtering draining, it is necessary to set special mistake for handling process and operating condition
Equipment is filtered, culture needs sludge and waste water being concomitantly introduced into filter plant after terminating, it is necessary to which filter cake is returned into culture dress after centrifugation
After putting resuspension, cultivated next time.After culture terminates, the sludge concentration cultivated in unit can reach 2000mg/L.Calculate
Always incubation time is：4h+10h+16h+23h+30h+39h=122h, each water discharge time about 2h, six total water discharge times are about
12h, therefore cultivate a collection of sludge and always take time about 134h.Although sludge concentration and incubation time can be ensured, due to needing
To run between different devices, operating process is relatively complicated.
By above-mentioned it is demonstrated experimentally that apparatus of the present invention carry out culturing sludge using ceramic Membrance cuiture unit, using vacuum mode
Draining, it can not only reduce the time in culturing sludge cycle, additionally it is possible to strengthen the clarity of water outlet, contribute to efficiently going for ammonia nitrogen
Except the steady growth with sludge.
Claims (11)
- A kind of 1. nitrifying bacteria enrichment culture apparatus, it is characterised in that including outer barrel reactor and ceramic membrane inner cylinder, outer barrel reactor For opened type structure, inside sets the ceramic membrane inner cylinder made of ceramic diaphragm plate；Aeration is set in ceramic membrane inner tank theca and bottom System, aerating system and sludge are located at ceramic membrane inner cylinder both sides respectively, and during aeration, sludge is cultivated in ceramic membrane inner cylinder, Gas enters inner cylinder by ceramic fenestra and realizes oxygen supply；External reactors bottom is provided with discharge outlet, passes through vavuum pump and sealing Header tank is connected, and after culture terminates, starts vavuum pump and water is drained into closed header tank, sludge is then by including ceramic membrane interception In cylinder.
- 2. device according to claim 1, it is characterised in that：The geometry of ceramic membrane inner cylinder is identical with outer barrel reactor Or it is different, inner cylinder volume is 0.5~0.8 times of outer barrel volume；The wall thickness of ceramic membrane inner cylinder is 1~5cm, film hole diameter 0.05 ~0.5 μm.
- 3. device according to claim 1, it is characterised in that：Aerating system is arranged at the outer surface of ceramic membrane inner cylinder, Ceramic membrane inner cylinder bottom is arranged in matts shape, is arranged on surrounding outer wall in horizontal bar；Aerating system and sludge are located at respectively The both sides of ceramic diaphragm plate, during aeration, gas passes through ceramic fenestra and realizes orientation oxygen supply.
- 4. using the nitrifying bacteria enrichment cultural method of any culture apparatuses of claim 1-3, it is characterised in that including following Step：（1）Nitrifier activated sludge is added in the ceramic membrane inner cylinder of culture apparatus；（2）Nutrient solution is injected into ceramic membrane inner cylinder so that influent ammonium concentration is 50~150mg/L, starts aerating system and exposes Gas；（3）When ammonia nitrogen concentration is less than 20mg/L, stop aeration, start vacuum pump drainage；Fresh medium is supplemented after draining, is adopted The mode for improving influent ammonium concentration step by step is taken to carry out batch cultivation so that final ammonia nitrogen concentration is improved to 500~600mg/L；（4）When ammonia nitrogen removal frank is up to more than 90%, stops aeration, vacuum drainage, collect nitrifying sludge.
- 5. according to the method for claim 4, it is characterised in that：Step（1）According to the volume of ceramic membrane inner cylinder, control culture The initial sludge concentration of system is 200~500mg/L.
- 6. according to the method for claim 4, it is characterised in that：Step（2）Controlled in cultivating system in ceramic membrane inner cylinder Dissolved oxygen concentration is 1.0~1.5mg/L, and temperature is 30~35 DEG C, and pH value is 7.0~9.0.
- 7. according to the method for claim 4, it is characterised in that：Step（2）The nutrient solution be autogamy nitrogen-containing wastewater or The good nitrogen-containing wastewater of caused various biodegradabilities in person's production and living, B/C ratios are not higher than 500mg/ more than 0.4, COD concentration L。
- 8. according to the method for claim 4, it is characterised in that：Additional nitration bacteria growing accelerator, described in incubation Accelerator includes metal salt and polyamines, and wherein metal salt is 40~100 parts by weight, and polyamines are 5~30 weight Part；Described metal salt is calcium salt, magnesium salts, molysite and mantoquita, Ca2+、Mg2+、Fe2+And Cu2+Mol ratio be（5~15）:（5~ 25）:（1~8）:（0.5~5）.
- 9. according to the method for claim 8, it is characterised in that：Metal salt is 50~80 parts by weight in the accelerator, more Amine substance is 10~20 parts by weight；Ca2+、Mg2+、Fe2+And Cu2+Mol ratio be（8~12）:（10~20）:（2~6）:（1 ~4）.
- 10. according to the method for claim 4, it is characterised in that：Step（3）Take the side for improving influent ammonium concentration step by step Formula carries out batch cultivation, and the increase rate of each ammonia nitrogen concentration is 50~100mg/L.
- 11. according to the method for claim 4, it is characterised in that：Step（3）After stopping aeration, the row of device for opening bottom The mouth of a river and vavuum pump, start vacuum pump drainage, the gauge pressure for adjusting vavuum pump is 0.1~0.2MPa.
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| CN111268793B (en) * | 2020-03-02 | 2022-05-10 | 浙江富春紫光环保股份有限公司 | Enrichment device and emergency feeding method of nitrobacteria for sewage treatment |
| CN111269837B (en) * | 2020-03-11 | 2022-04-05 | 天津创业环保集团股份有限公司 | Sequencing batch anaerobic ammonium oxidation bacterium culture method and device |
| CN112174306B (en) * | 2020-10-28 | 2022-01-04 | 深圳中清环境科技有限公司 | Ceramic membrane sewage treatment equipment and method based on domesticated active microorganisms |
| CN113264587A (en) * | 2021-06-02 | 2021-08-17 | 知和环保科技有限公司 | Culture method of high-load efficient nitrifying bacteria |
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| CN1123769A (en) * | 1994-11-30 | 1996-06-05 | 中国石油化工总公司抚顺石油化工研究院 | Treatment for organic wastewater containing ammonia from oil refinery |
| CN1424265A (en) * | 2003-01-17 | 2003-06-18 | 清华大学 | Process and apparatus for wastewater by batched membrane-bioreactor |
| CN101709278A (en) * | 2009-11-23 | 2010-05-19 | 浙江省环境保护科学设计研究院 | Large-scaled culture method of high-concentration nitrosobacteria and application thereof |
| CN102952765A (en) * | 2011-08-31 | 2013-03-06 | 中国石油化工股份有限公司 | Enrichment culture method of salt-tolerant efficient nitrosobacteria |
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| KR100769659B1 (en) * | 2007-04-25 | 2007-10-23 | 강민기 | Microbe cultivation apparauts |
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| CN1123769A (en) * | 1994-11-30 | 1996-06-05 | 中国石油化工总公司抚顺石油化工研究院 | Treatment for organic wastewater containing ammonia from oil refinery |
| CN1424265A (en) * | 2003-01-17 | 2003-06-18 | 清华大学 | Process and apparatus for wastewater by batched membrane-bioreactor |
| CN101709278A (en) * | 2009-11-23 | 2010-05-19 | 浙江省环境保护科学设计研究院 | Large-scaled culture method of high-concentration nitrosobacteria and application thereof |
| CN102952765A (en) * | 2011-08-31 | 2013-03-06 | 中国石油化工股份有限公司 | Enrichment culture method of salt-tolerant efficient nitrosobacteria |
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