CN105709898A - Method for obtaining grape hybrid fruit seeds - Google Patents
Method for obtaining grape hybrid fruit seeds Download PDFInfo
- Publication number
- CN105709898A CN105709898A CN201610063878.9A CN201610063878A CN105709898A CN 105709898 A CN105709898 A CN 105709898A CN 201610063878 A CN201610063878 A CN 201610063878A CN 105709898 A CN105709898 A CN 105709898A
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- seed
- fruit
- seeds
- hybrid grape
- clear water
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C18/00—Disintegrating by knives or other cutting or tearing members which chop material into fragments
- B02C18/06—Disintegrating by knives or other cutting or tearing members which chop material into fragments with rotating knives
- B02C18/16—Details
- B02C18/18—Knives; Mountings thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
- B08B3/10—Cleaning involving contact with liquid with additional treatment of the liquid or of the object being cleaned, e.g. by heat, by electricity or by vibration
- B08B3/102—Cleaning involving contact with liquid with additional treatment of the liquid or of the object being cleaned, e.g. by heat, by electricity or by vibration with means for agitating the liquid
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- Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Medicines Containing Plant Substances (AREA)
- Storage Of Fruits Or Vegetables (AREA)
- Preparation Of Fruits And Vegetables (AREA)
Abstract
The invention discloses a method for obtaining grape hybrid fruit seeds, and relates to the technical field of fruit breeding. The method comprises the following steps of obtaining grape hybrid seedling fruits from trees, and putting the grape hybrid seedling fruits at a cool and dry place for after-ripening so as to obtain grape seedling fruits; putting the seedling fruits in a pulp refiner to carry out grinding treatment until the seedling fruits are completely grinded into a mixture of seeds, pulps and peels; filtering the mixture to obtain a seed-containing initial extract; washing the initial extract by clear water until the waste water after the washing is clear water, so as to obtain seeds of the grape hybrid fruits; and air-drying the seeds, and carrying out sand storage and stratification on the seeds and wet sand so as to complete the obtaining of the grape hybrid fruit seeds. The method disclosed in the invention has the advantages of being simple, convenient, rapid and effective and yielding twice the result with half the effort.
Description
Technical field
The present invention relates to fruit breeding technical field, particularly relate to a kind of method obtaining Hybrid Grape fruit and seed.
Background technology
Fructus Vitis viniferae, as one of most important fruit tree species of China, both can eat raw, can make wine again, make juice dry, processed and tank processed, simultaneously as its strong adaptability, result early, high efficiency, make Fructus Vitis viniferae industry have become as the special advantage industries in the many places of China and pillar industry that increasing peasant income are got rich.In the production process of Fructus Vitis viniferae, excellent grape variety is the essential condition improving grape quality and planting benefit, also it is the important leverage of its industry sustainable development, look back the development course of China's Fructus Vitis viniferae industry, fast-developing all along with the introduction of new varieties, selection-breeding and popularization each time, new varieties are to promoting that the development that Fructus Vitis viniferae produces plays more and more important effect.
Cross-breeding is the conventional route of grape breeding, is also one of most widely used general and effective breeding method on current fruit tree both at home and abroad.First specific parent is selected according to breeding objective, then pass through control emasculation, pollination link makes the combination of Parent gene obtain true seed, after seed carries out Stratificated treatment, accelerating germination is sowed, and then obtain hybridization seedling, seedling is carried out primary election, final election and final election, finally selects the new grape variety meeting people's target.Since 2000, the new grape variety that cross breeding method is cultivated has been utilized to account for more than the 70% of China's selection-breeding new grape variety, it may be said that cross-breeding is the main path of China's new grape variety selection-breeding.
In cross-breeding process, the current domestic methods adopting craft that obtain of true seed directly strip out more from the fruit that grows directly from seeds of hybridization, next one seed that only can strip more than 5000 grains working day of individual of ordinary circumstance, efficiency is low, speed is slow, it is difficult to meet the demand of modernization breeding.
Summary of the invention
It is an object of the invention to provide a kind of method obtaining Hybrid Grape fruit and seed, thus solving the foregoing problems existed in prior art.
To achieve these goals, the method for acquisition Hybrid Grape fruit and seed of the present invention, the method includes:
S1, obtains Hybrid Grape from tree body and grows directly from seeds fruit, is placed on shady and cool dry place's after-ripening, obtains Fructus Vitis viniferae and grows directly from seeds fruit；
S2, fruit of being grown directly from seeds by Fructus Vitis viniferae is placed in refiner and carries out break process, till fruit of growing directly from seeds is completely broken, obtains the mixture of seed, sarcocarp and peel；
S3, filtering mixt, obtain comprising the first extract of seed；
S4, rinses first extract clear water, till the waste water after rinsing is clear water, obtains the seed of Hybrid Grape fruit；
S5, by air-dry for seed dry in the sun, then carry out husky Tibetan Stratificated treatment together with wet sand, completes the acquisition of Hybrid Grape fruit and seed.
Preferably, the grow directly from seeds color of fruit and seed of described Hybrid Grape is brown.
Preferably, in step S2, during described break process, the speed of refiner is 600r/min-800r/min, and the time of homogenate is 2min-3min.
Preferably, the blade installed in described refiner is cross angled blade.
Preferably, filter concrete realization as steps described below described in step S3: select the aperture screen cloth at least above 8mm to filter the bulk sarcocarp in mixture and peel, obtain comprising seed just extract.
Preferably, step S4, specifically realize as steps described below:
S41, will comprise seed just extract and be placed in container, and add clear water, be sufficiently stirred in described container, and remove filtrate, obtain solids after filtration, then by solids again by clean water till the filtrate obtained is clear water, obtain primary election seed；
S42, is placed in clear water by primary election seed, stands 2min-3min, obtains the seed sunk to bottom clear water, as target seed, enters S5.
Preferably, in step S5, the air-dry seed moisture content of dry in the sun is 4%-6%.
Preferably, in step S5, the humidity of described wet sand is 5%～10%.
Preferably, in step S5, the husky temperature hiding Stratificated treatment is 2 DEG C～5 DEG C, hides the Stratificated treatment later stage at sand, is both needed to weekly stir seed 1 time.
The invention has the beneficial effects as follows:
The present invention utilizes refiner and mesh screen Fructus Vitis viniferae is grown directly from seeds fruit to carry out crushing and filtering, and obtains clean and full Hybrid Grape true seed, improves Hybrid Grape seed and obtains efficiency.Compared with prior art, the present invention has simple and convenient, quickly effectively and the advantage got twice the result with half the effort, and present disclosure additionally applies for obtaining other and grows directly from seeds the quick obtaining of fruit and seed such as hybridization such as Fructus actinidiae chinensis, Fructus Fragariae Ananssae, Fructus Mali pumilae, pears.
Accompanying drawing explanation
Fig. 1 is the schematic flow sheet of the method obtaining Hybrid Grape fruit and seed.
Detailed description of the invention
In order to make the purpose of the present invention, technical scheme and advantage clearly understand, below in conjunction with accompanying drawing, the present invention is further elaborated.Should be appreciated that detailed description of the invention described herein is only in order to explain the present invention, is not intended to limit the present invention.
Embodiment
The method obtaining Hybrid Grape fruit and seed described in the present embodiment, the method includes:
S1, obtains Hybrid Grape from tree body and grows directly from seeds fruit, is placed on shady and cool dry place's after-ripening, obtains Fructus Vitis viniferae and grows directly from seeds fruit；
S2, fruit of being grown directly from seeds by Fructus Vitis viniferae is placed in refiner and carries out break process, till fruit of growing directly from seeds is completely broken, obtains the mixture of seed, sarcocarp and peel；
S3, filtering mixt, obtain comprising the first extract of seed；
S4, rinses first extract clear water, till the waste water after rinsing is clear water, obtains the seed of Hybrid Grape fruit；
S5, by air-dry for seed dry in the sun, then carry out husky Tibetan Stratificated treatment together with wet sand, completes the acquisition of Hybrid Grape fruit and seed.
It is explained in more detail explanation:
(1) Hybrid Grape grows directly from seeds fruit full maturity, and the grow directly from seeds color of fruit and seed of Hybrid Grape is brown.
(2) in step S2, during described break process, the speed of refiner is 600r/min-800r/min, and the time of homogenate is 2min-3min.The blade installed in described refiner is cross angled blade.
The fruit that will grow directly from seeds is put in refiner, broken fruit, makes seed and sarcocarp, peel be easily separated, notices that homogenate speed can not be too high, and the time is unsuitable long, and homogenate blade is not wanted too sharp, it is prevented that is damaged by seed, is broken for fruit suitable completely.
(3) filter concrete realization as steps described below described in step S3: select the aperture screen cloth at least above 8mm to filter the bulk sarcocarp in mixture and peel, obtain comprising seed just extract.Most sarcocarp and peel is removed by the mesh screen of different pore size size, obtaining the mixed liquid of seed and a small amount of sarcocarp, note different types of hybridization fruit, seed size is different, the aperture of the mesh screen required is also just different, it is necessary to select suitable mesh screen according to the size of seed.
(4) step S4, specifically realizes as steps described below:
S41, will comprise seed just extract and be placed in container, and add clear water, be sufficiently stirred in described container, and remove filtrate, obtain solids after filtration, then by solids again by clean water till the filtrate obtained is clear water, obtain primary election seed；
S42, is placed in clear water by primary election seed, stands 2min-3min, obtains the seed sunk to bottom clear water, as target seed, enters S5.Impurity and not full exalbuminose seed swim in top.
(5), in step S5, the air-dry seed moisture content of dry in the sun is 4%-6%.
In step S5, the humidity of described wet sand is 5%～10%.The humidity of described wet sand is not dripped to hold agglomerating, and one touches namely scattered being as the criterion.
In step S5, the husky temperature hiding Stratificated treatment is 2 DEG C～5 DEG C, hides the Stratificated treatment later stage at sand, it is necessary to notes running check, is both needed to weekly stir seed 1 time, in case it is rotten to go rotten.Accelerating germination sowing in next year is awaited after sand hides Stratificated treatment.
By adopting technique scheme disclosed by the invention, obtain following beneficial effect: the present invention utilizes refiner and mesh screen Fructus Vitis viniferae is grown directly from seeds fruit to carry out crushing and filtering, obtain clean and full Hybrid Grape true seed, improve Hybrid Grape seed and obtain efficiency.Compared with prior art, the present invention has simple and convenient, quickly effectively and the advantage got twice the result with half the effort, and present disclosure additionally applies for obtaining other and grows directly from seeds the quick obtaining of fruit and seed such as hybridization such as Fructus actinidiae chinensis, Fructus Fragariae Ananssae, Fructus Mali pumilae, pears.
The above is only the preferred embodiment of the present invention; it should be pointed out that, for those skilled in the art, under the premise without departing from the principles of the invention; can also making some improvements and modifications, these improvements and modifications also should look protection scope of the present invention.
Claims (9)
1. the method obtaining Hybrid Grape fruit and seed, it is characterised in that the method includes:
S1, obtains Hybrid Grape from tree body and grows directly from seeds fruit, is placed on shady and cool dry place's after-ripening, obtains Fructus Vitis viniferae and grows directly from seeds fruit；
S2, fruit of being grown directly from seeds by Fructus Vitis viniferae is placed in refiner and carries out break process, till fruit of growing directly from seeds is completely broken, obtains the mixture of seed, sarcocarp and peel；
S3, filtering mixt, obtain comprising the first extract of seed；
S4, rinses first extract clear water, till the waste water after rinsing is clear water, obtains the seed of Hybrid Grape fruit；
S5, by air-dry for seed dry in the sun, then carry out husky Tibetan Stratificated treatment together with wet sand, completes the acquisition of Hybrid Grape fruit and seed.
2. the method obtaining Hybrid Grape fruit and seed according to claim 1, it is characterised in that the color of the real carpogenic seed of described Hybrid Grape is brown.
3. the method obtaining Hybrid Grape fruit and seed according to claim 1, it is characterised in that in step S2, during described break process, the speed of refiner is 600r/min-800r/min, and the time of homogenate is 2min-3min.
4. the method obtaining Hybrid Grape fruit and seed according to claim 1, it is characterised in that the blade installed in described refiner is cross angled blade.
5. the method obtaining Hybrid Grape fruit and seed according to claim 1, it is characterised in that filter concrete realization as steps described below described in step S3:
Select the aperture screen cloth at least above 8mm to filter the bulk sarcocarp in mixture and peel, obtain comprising seed just extract.
6. the method obtaining Hybrid Grape fruit and seed according to claim 1, it is characterised in that step S4, specifically realizes as steps described below:
S41, will comprise seed just extract and be placed in container, and add clear water, be sufficiently stirred in described container, and remove filtrate, obtain solids after filtration, then by solids again by clean water till the filtrate obtained is clear water, obtain primary election seed；
S42, is placed in clear water by primary election seed, stands 2min-3min, obtains the seed sunk to bottom clear water, as target seed, enters S5.
7. the method obtaining Hybrid Grape fruit and seed according to claim 1, it is characterised in that in step S5, the air-dry seed moisture content of dry in the sun is 4%-6%.
8. the method obtaining Hybrid Grape fruit and seed according to claim 1, it is characterised in that in step S5, the humidity of described wet sand is 5%～10%.
9. the method obtaining Hybrid Grape fruit and seed according to claim 1, it is characterised in that in step S5, the husky temperature hiding Stratificated treatment is 2 DEG C～5 DEG C, hides the Stratificated treatment later stage at sand, is both needed to weekly stir seed 1 time.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610063878.9A CN105709898B (en) | 2016-01-29 | 2016-01-29 | A kind of method for obtaining Hybrid Grape fruit and seed |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610063878.9A CN105709898B (en) | 2016-01-29 | 2016-01-29 | A kind of method for obtaining Hybrid Grape fruit and seed |
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| Publication Number | Publication Date |
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| CN105709898A true CN105709898A (en) | 2016-06-29 |
| CN105709898B CN105709898B (en) | 2018-06-29 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610063878.9A Expired - Fee Related CN105709898B (en) | 2016-01-29 | 2016-01-29 | A kind of method for obtaining Hybrid Grape fruit and seed |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106385867A (en) * | 2016-09-29 | 2017-02-15 | 中国科学院昆明植物研究所 | Cleaning method of saussurea obvallata fruits |
| CN114762472A (en) * | 2020-12-30 | 2022-07-19 | 北京林业大学 | Method for germinating vitis amurensis seeds and application thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101438644A (en) * | 2008-12-26 | 2009-05-27 | 四川师范大学 | Method for treating seed for seedling culture of Idesia polycarpa Maxim. var. vestita Diels |
| CN101507383A (en) * | 2009-04-03 | 2009-08-19 | 湖北省农业科学院果树茶叶研究所 | Germinating breeding method of Chinese pear cross-breed |
| MD508Y (en) * | 2012-01-06 | 2012-05-31 | Inst De Genetica Si Fiziol A Plantelor Al Academiei De Stiinte A Moldovei | Process for presowing treatment of grape seeds |
| CN103329662A (en) * | 2013-07-25 | 2013-10-02 | 北京市农林科学院林业果树研究所 | Sowing and seedling raising method for grape hybrid seeds |
| CN104012206A (en) * | 2013-03-03 | 2014-09-03 | 四川农业大学 | Method for rapidly obtaining seeds from mature nightshade fruits |
| CN105028082A (en) * | 2015-06-17 | 2015-11-11 | 中国林业科学研究院亚热带林业研究所 | Common sassafras seed treating and sowing method |
-
2016
- 2016-01-29 CN CN201610063878.9A patent/CN105709898B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101438644A (en) * | 2008-12-26 | 2009-05-27 | 四川师范大学 | Method for treating seed for seedling culture of Idesia polycarpa Maxim. var. vestita Diels |
| CN101507383A (en) * | 2009-04-03 | 2009-08-19 | 湖北省农业科学院果树茶叶研究所 | Germinating breeding method of Chinese pear cross-breed |
| MD508Y (en) * | 2012-01-06 | 2012-05-31 | Inst De Genetica Si Fiziol A Plantelor Al Academiei De Stiinte A Moldovei | Process for presowing treatment of grape seeds |
| CN104012206A (en) * | 2013-03-03 | 2014-09-03 | 四川农业大学 | Method for rapidly obtaining seeds from mature nightshade fruits |
| CN103329662A (en) * | 2013-07-25 | 2013-10-02 | 北京市农林科学院林业果树研究所 | Sowing and seedling raising method for grape hybrid seeds |
| CN105028082A (en) * | 2015-06-17 | 2015-11-11 | 中国林业科学研究院亚热带林业研究所 | Common sassafras seed treating and sowing method |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106385867A (en) * | 2016-09-29 | 2017-02-15 | 中国科学院昆明植物研究所 | Cleaning method of saussurea obvallata fruits |
| CN106385867B (en) * | 2016-09-29 | 2019-11-19 | 中国科学院昆明植物研究所 | The method for cleaning of Saus-surea obvallata (DC.) Edgew fruit |
| CN114762472A (en) * | 2020-12-30 | 2022-07-19 | 北京林业大学 | Method for germinating vitis amurensis seeds and application thereof |
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| Publication number | Publication date |
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| CN105709898B (en) | 2018-06-29 |
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Granted publication date: 20180629 Termination date: 20190129 |