CN105707765A - Liver protection plant oral liquid and preparation method thereof - Google Patents
Liver protection plant oral liquid and preparation method thereof Download PDFInfo
- Publication number
- CN105707765A CN105707765A CN201610083663.3A CN201610083663A CN105707765A CN 105707765 A CN105707765 A CN 105707765A CN 201610083663 A CN201610083663 A CN 201610083663A CN 105707765 A CN105707765 A CN 105707765A
- Authority
- CN
- China
- Prior art keywords
- liquid
- plant
- radix puerariae
- oral liquid
- hepatoprotective
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000007788 liquid Substances 0.000 title claims abstract description 364
- 238000002360 preparation method Methods 0.000 title claims abstract description 54
- 210000004185 liver Anatomy 0.000 title abstract description 34
- 241000196324 Embryophyta Species 0.000 claims abstract description 217
- 239000002994 raw material Substances 0.000 claims abstract description 118
- 238000000855 fermentation Methods 0.000 claims abstract description 87
- 230000004151 fermentation Effects 0.000 claims abstract description 87
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 80
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 40
- 239000008103 glucose Substances 0.000 claims abstract description 40
- 230000001954 sterilising effect Effects 0.000 claims abstract description 28
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 8
- 230000002443 hepatoprotective effect Effects 0.000 claims description 127
- 230000005070 ripening Effects 0.000 claims description 54
- 239000000706 filtrate Substances 0.000 claims description 38
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 36
- 239000000463 material Substances 0.000 claims description 35
- 241000218588 Lactobacillus rhamnosus Species 0.000 claims description 28
- 241000209051 Saccharum Species 0.000 claims description 27
- 238000003756 stirring Methods 0.000 claims description 27
- 244000199885 Lactobacillus bulgaricus Species 0.000 claims description 26
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 claims description 26
- 244000199866 Lactobacillus casei Species 0.000 claims description 26
- 235000013958 Lactobacillus casei Nutrition 0.000 claims description 26
- 241000194020 Streptococcus thermophilus Species 0.000 claims description 26
- 229940004208 lactobacillus bulgaricus Drugs 0.000 claims description 26
- 229940017800 lactobacillus casei Drugs 0.000 claims description 26
- 241000186016 Bifidobacterium bifidum Species 0.000 claims description 23
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 23
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 23
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 23
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 23
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 23
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 22
- 108010059892 Cellulase Proteins 0.000 claims description 22
- 229940106157 cellulase Drugs 0.000 claims description 22
- 238000012545 processing Methods 0.000 claims description 22
- 229930003270 Vitamin B Natural products 0.000 claims description 17
- 235000019156 vitamin B Nutrition 0.000 claims description 17
- 239000011720 vitamin B Substances 0.000 claims description 17
- 108091005508 Acid proteases Proteins 0.000 claims description 15
- 239000004382 Amylase Substances 0.000 claims description 15
- 102000013142 Amylases Human genes 0.000 claims description 15
- 108010065511 Amylases Proteins 0.000 claims description 15
- 235000019418 amylase Nutrition 0.000 claims description 15
- 210000000582 semen Anatomy 0.000 claims description 15
- 239000000428 dust Substances 0.000 claims description 14
- 238000005374 membrane filtration Methods 0.000 claims description 14
- 229940023492 oral liquid product Drugs 0.000 claims description 14
- 238000007789 sealing Methods 0.000 claims description 14
- 239000002253 acid Substances 0.000 claims description 12
- 235000001014 amino acid Nutrition 0.000 claims description 12
- 229940024606 amino acid Drugs 0.000 claims description 12
- 150000001413 amino acids Chemical class 0.000 claims description 12
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 12
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 11
- 240000000588 Hericium erinaceus Species 0.000 claims description 11
- 235000007328 Hericium erinaceus Nutrition 0.000 claims description 11
- 240000000599 Lentinula edodes Species 0.000 claims description 11
- 235000001715 Lentinula edodes Nutrition 0.000 claims description 11
- 229930003268 Vitamin C Natural products 0.000 claims description 11
- 244000309464 bull Species 0.000 claims description 11
- 235000019154 vitamin C Nutrition 0.000 claims description 11
- 239000011718 vitamin C Substances 0.000 claims description 11
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 10
- 241000220324 Pyrus Species 0.000 claims description 10
- 235000021017 pears Nutrition 0.000 claims description 10
- 239000004475 Arginine Substances 0.000 claims description 5
- 241000233866 Fungi Species 0.000 claims description 5
- 239000004471 Glycine Substances 0.000 claims description 5
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims description 5
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 claims description 5
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 claims description 5
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 claims description 5
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims description 5
- 229960000310 isoleucine Drugs 0.000 claims description 5
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 claims description 5
- 238000010129 solution processing Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 11
- 239000000203 mixture Substances 0.000 abstract description 11
- 239000003814 drug Substances 0.000 abstract description 3
- 238000002156 mixing Methods 0.000 abstract description 3
- 235000019640 taste Nutrition 0.000 abstract description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 2
- 238000001723 curing Methods 0.000 abstract 2
- 238000011049 filling Methods 0.000 abstract 2
- 238000001914 filtration Methods 0.000 abstract 2
- 238000004659 sterilization and disinfection Methods 0.000 abstract 2
- 241000675108 Citrus tangerina Species 0.000 abstract 1
- 235000009917 Crataegus X brevipes Nutrition 0.000 abstract 1
- 235000013204 Crataegus X haemacarpa Nutrition 0.000 abstract 1
- 235000009685 Crataegus X maligna Nutrition 0.000 abstract 1
- 235000009444 Crataegus X rubrocarnea Nutrition 0.000 abstract 1
- 235000009486 Crataegus bullatus Nutrition 0.000 abstract 1
- 235000017181 Crataegus chrysocarpa Nutrition 0.000 abstract 1
- 235000009682 Crataegus limnophila Nutrition 0.000 abstract 1
- 235000004423 Crataegus monogyna Nutrition 0.000 abstract 1
- 240000000171 Crataegus monogyna Species 0.000 abstract 1
- 235000002313 Crataegus paludosa Nutrition 0.000 abstract 1
- 235000009840 Crataegus x incaedua Nutrition 0.000 abstract 1
- 244000046146 Pueraria lobata Species 0.000 abstract 1
- 235000010575 Pueraria lobata Nutrition 0.000 abstract 1
- 240000004922 Vigna radiata Species 0.000 abstract 1
- 235000010721 Vigna radiata var radiata Nutrition 0.000 abstract 1
- 235000011469 Vigna radiata var sublobata Nutrition 0.000 abstract 1
- 239000004480 active ingredient Substances 0.000 abstract 1
- 235000018927 edible plant Nutrition 0.000 abstract 1
- 235000012055 fruits and vegetables Nutrition 0.000 abstract 1
- 235000021552 granulated sugar Nutrition 0.000 abstract 1
- 230000002195 synergetic effect Effects 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 33
- 239000003795 chemical substances by application Substances 0.000 description 24
- 238000000034 method Methods 0.000 description 8
- 241000699670 Mus sp. Species 0.000 description 7
- 238000003304 gavage Methods 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 6
- 208000007848 Alcoholism Diseases 0.000 description 5
- 201000007930 alcohol dependence Diseases 0.000 description 5
- 241000699666 Mus <mouse, genus> Species 0.000 description 4
- 230000037396 body weight Effects 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 238000009472 formulation Methods 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 230000007812 deficiency Effects 0.000 description 3
- 230000003203 everyday effect Effects 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 229930003231 vitamin Natural products 0.000 description 3
- 235000013343 vitamin Nutrition 0.000 description 3
- 239000011782 vitamin Substances 0.000 description 3
- 229940088594 vitamin Drugs 0.000 description 3
- -1 Compound vitamin Chemical class 0.000 description 2
- WSMYVTOQOOLQHP-UHFFFAOYSA-N Malondialdehyde Chemical compound O=CCC=O WSMYVTOQOOLQHP-UHFFFAOYSA-N 0.000 description 2
- 235000019658 bitter taste Nutrition 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 235000008216 herbs Nutrition 0.000 description 2
- 231100000957 no side effect Toxicity 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 230000008520 organization Effects 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- RXUWDKBZZLIASQ-UHFFFAOYSA-N Puerarin Natural products OCC1OC(Oc2c(O)cc(O)c3C(=O)C(=COc23)c4ccc(O)cc4)C(O)C(O)C1O RXUWDKBZZLIASQ-UHFFFAOYSA-N 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 210000000514 hepatopancreas Anatomy 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 230000003908 liver function Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000004570 mortar (masonry) Substances 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- HKEAFJYKMMKDOR-VPRICQMDSA-N puerarin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1C1=C(O)C=CC(C2=O)=C1OC=C2C1=CC=C(O)C=C1 HKEAFJYKMMKDOR-VPRICQMDSA-N 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/123—Bulgaricus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/125—Casei
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/175—Rhamnosus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/21—Streptococcus, lactococcus
- A23V2400/249—Thermophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
Provided is liver protection plant oral liquid.The oral liquid is mainly prepared from hawthorn fruits, red dates, mung beans, dried tangerine or orange peel, the root of kudzu vine, glucose, brown granulated sugar and water.The invention further discloses one preparation method of the liver protection plant oral liquid.The preparation method comprises the following steps of raw material treatment, enzymolysis, fermentation, filtering and curing, mixing and filling and sterilization.The invention further discloses the other preparation of the liver protection plant oral liquid.The preparation method comprises the following steps of raw material treatment, enzymolysis, primary fermentation, secondary fermentation, filtering and curing, mixing and filling and sterilization.Compared with the prior art, the medicinal and edible plant raw materials and multiple fruits and vegetables are preferred, the synergistic effect of the raw materials is fully achieved through the optimum mixture ratio and the preparation methods, active ingredients in the raw materials are released better, and the prepared liver protection plant oral liquid is fragrant and fresh in taste and easy to drink, has the remarkable effects of protecting the liver and dispelling the effects of alcohol, is free of side effects and can meet the needs of normal people who do not want to take medicine for liver protection and alcohol effect dispelling.
Description
Technical field
The invention belongs to health product technology field, relate to a kind of oral liquid, particularly a kind of hepatoprotective plant oral liquid and preparation method thereof.
Background technology
In recent years, along with the pressure of modern life fast development people is increasing, the hepatopaths such as the people drunk also gets more and more, alcoholic liver also increase many.Research shows, the sub-health state of modern has the reason of more than half to come from " deficiency of the liver and kindey ", wherein, liver is one of most important organ of human body, also it is digestive gland maximum in human body, being the most vigorous organ of metabolism, the quality of liver directly affects the running of whole physical function, so hepatoprotective is particularly significant.At present, the liver-protection health-care product that market exists are of a great variety, but are Chinese traditional herbs preparation mostly, it is impossible to the liver-protecting and alcoholism-relieving demand of the satisfied normal person being not desired to take medicine；And wherein still mix and do not possess liver-protecting function even there is the product of inferior quality of side effect, do not reach desirable hepatoprotective effect.
Summary of the invention
The technical problem to be solved is for the deficiencies in the prior art, it is provided that the hepatoprotective plant oral liquid that a kind of compatibility rationally, easily absorbs and has no side effect.
To be solved by this invention another technical problem is that a kind of preparation method additionally providing aforementioned hepatoprotective plant oral liquid.
It is to be solved by this invention that another technical problem is that the another kind of preparation method additionally providing aforementioned hepatoprotective plant oral liquid.
The technical problem to be solved is to be realized by following technical scheme.The present invention is a kind of hepatoprotective plant oral liquid, is characterized in, primary raw material and the weight proportion thereof of making this oral liquid include:
Fructus Crataegi 1 ~ 10；Fructus Jujubae 1 ~ 10；Semen phaseoli radiati 1 ~ 10；Pericarpium Citri Reticulatae 0.5 ~ 5；
Radix Puerariae 5 ~ 15；Glucose 2 ~ 8；Brown sugar (Saccharum Sinensis Roxb.) 3 ~ 12；Water 65 ~ 85.
Hepatoprotective plant oral liquid of the present invention: the weight proportion of each raw material is preferably:
Fructus Crataegi 2；Fructus Jujubae 2；Semen phaseoli radiati 2；Pericarpium Citri Reticulatae 1；
Radix Puerariae 10；Glucose 4；Brown sugar (Saccharum Sinensis Roxb.) 6；Water 73.
Hepatoprotective plant oral liquid of the present invention, further, the raw material making this oral liquid can also include the edible fungi of following weight proportion: Hericium erinaceus (Bull. Ex Fr.) Pers. 5 ~ 15；Lentinus Edodes 5 ~ 10；The weight proportion of described Hericium erinaceus (Bull. Ex Fr.) Pers. more preferably 6；The weight proportion of described Lentinus Edodes more preferably 6.
Hepatoprotective plant oral liquid of the present invention, further, the raw material making this oral liquid can also include one or more fruit and vegerable of following weight proportion: Fructus Musae 1 ~ 5；Pears 1 ~ 5；Fructus Lycopersici esculenti 1 ~ 5；Fructus Citri junoris 1 ~ 5；Fresh Rhizoma Nelumbinis 1 ~ 5；Herba Apii graveolentis 1 ~ 5；The weight proportion of described Fructus Musae more preferably 2；The weight proportion of described pears more preferably 2；The weight proportion of described Fructus Lycopersici esculenti more preferably 2；The weight proportion of described Fructus Citri junoris more preferably 2；The weight proportion of described fresh Rhizoma Nelumbinis more preferably 2；The weight proportion of described Herba Apii graveolentis more preferably 2.
Hepatoprotective plant oral liquid of the present invention, further, when vitamin B, Vitamin C content deficiency in detecting described hepatoprotective plant oral liquid, the raw material making this oral liquid can also include vitamin B, vitamin C, make the content of vitamin B in described hepatoprotective plant oral liquid reach 0.05 ~ 0.2mg/100g, make ascorbic content in described hepatoprotective plant oral liquid reach 10 ~ 100mg/100g；The content more preferably 0.1mg/100g of vitamin B in described hepatoprotective plant oral liquid；Ascorbic content more preferably 100mg/100g in described hepatoprotective plant oral liquid.
Vitamin B of the present invention, vitamin C can adopt the product of commercially available any dosage form.
Hepatoprotective plant oral liquid of the present invention, further, make one or more aminoacid that the raw material of this oral liquid can also include in glycine, arginine, leucine, isoleucine, make the content of described hepatoprotective plant Amino Acids in Oral Liquid reach 50 ~ 500mg/100g；The content more preferably 100mg/100g of described hepatoprotective plant Amino Acids in Oral Liquid.
The technical problem to be solved can also be realized further by following technical scheme.Present invention also offers a kind of preparation method of hepatoprotective plant oral liquid described in one technical scheme of any of the above, be characterized in, it comprises the steps:
(1) Feedstock treating: clean and avoid Radix Puerariae to soak in water the plant raw material in above-mentioned raw materials with water, puts into refiner and adds water homogenate than 1:4 by material water quality, obtain homogenate after draining；
(2) enzymolysis: be separately added in described homogenate and respectively account for the amylase of its quality 0.1 ~ 1%, pectase and cellulase, enzymolysis 4 ~ 16h at 37 ~ 45 DEG C, obtain primary enzymolysis liquid；In primary enzymolysis liquid, add the acid protease accounting for its quality 0.1 ~ 1%, enzymolysis 4 ~ 10h at 37 ~ 45 DEG C, obtain secondary enzymolysis liquid；
(3) fermentation: adding the glucose in above-mentioned raw materials and brown sugar (Saccharum Sinensis Roxb.) in described secondary enzymolysis liquid, add water, stirring to sugar is completely dissolved, then is added to 0.1 × 109~1×109The leaven of CFU/L, reaches 3 ~ 4 at 37 ~ 40 DEG C of bottom fermentations to material liquid pH value, obtains fermentation liquid；
Described leaven is the combination strain being made up of two or three in Lactobacillus bulgaricus, lactobacillus casei, streptococcus thermophilus, bacillus bifidus, Lactobacillus plantarum, lactobacillus rhamnosus, bacillus acidophilus；
(4) filter, ripening: be filtered described fermentation liquid processing, filtrate be placed at 4 DEG C ~ 10 DEG C ripening at low temperature 4 ~ 60 days, obtain ripening liquid；
(5) allotment: add other raw materials except plant raw material and sugar, stirring and dissolving in described ripening liquid, adds appropriate citric acid and glucose regulates sugar-acid ratio, moderate to mouthfeel；
(6) fill sterilizing: liquid deployed in step (5) is carried out fill, sealing at dust proof workshop with 0.45 μm of membrane filtration and by every bottle of 30ml specification, then at 80 ~ 120 DEG C sterilizing 20 ~ 30min, namely obtain hepatoprotective plant oral liquid product.
In a kind of preparation method of hepatoprotective plant oral liquid of the present invention, it is preferred that technical scheme or technology is characterized in that
1., in step (2), it is separately added in described homogenate and respectively accounts for the amylase of its quality 0.1%, pectase and cellulase, enzymolysis 4h at 45 DEG C, obtain primary enzymolysis liquid；In primary enzymolysis liquid, add the acid protease accounting for its quality 0.1%, enzymolysis 4h at 40 DEG C, obtain secondary enzymolysis liquid.
2. in step (3), adding the sugar in above-mentioned raw materials and water in described secondary enzymolysis liquid, stirring to sugar is completely dissolved, then is added to 1 × 109The leaven of CFU/L, reaches 3.5 at 37 DEG C of bottom fermentations to material liquid pH value, obtains fermentation liquid.
3. in step (3), described leaven is mass ratio is Lactobacillus bulgaricus: lactobacillus casei: streptococcus thermophilus=2:1.5:1, or mass ratio is bacillus bifidus: Lactobacillus plantarum: the combination strain of lactobacillus rhamnosus=2:2:1.
4., in step (4), it is filtered described fermentation lixiviating solution processing, is placed at 6 DEG C by the fermentation liquid being filtrated to get ripening at low temperature 10 days, obtains ripening liquid.
5., in step (6), liquid deployed for step (5) is carried out fill, sealing at dust proof workshop with 0.45 μm of membrane filtration and by every bottle of 30ml specification, then at 85 DEG C sterilizing 30min, namely obtain hepatoprotective plant oral liquid product.
The technical problem to be solved can also be realized further by following technical scheme.Present invention also offers the another kind of preparation method of hepatoprotective plant oral liquid described in one technical scheme of any of the above, be characterized in, it comprises the steps:
(1) Feedstock treating: clean and avoid Radix Puerariae to soak in water the plant raw material in above-mentioned raw materials with water, after draining, Radix Puerariae and the other plant class raw material except Radix Puerariae are separately used refiner homogenate, all add water homogenate than 1:4 by material water quality during homogenate, respectively obtain Radix Puerariae homogenate and the mixed plant homogenate except Radix Puerariae；
(2) enzymolysis: be separately added in described Radix Puerariae homogenate and respectively account for the amylase of its quality 0.1 ~ 1%, cellulase, it is separately added in the described mixed plant homogenate except Radix Puerariae and respectively accounts for the pectase of its quality 0.1 ~ 1%, cellulase, enzymolysis 4 ~ 16h at 37 ~ 45 DEG C, obtains Radix Puerariae primary enzymolysis liquid and the mixed plant primary enzymolysis liquid except Radix Puerariae；In above two primary enzymolysis liquid, add the acid protease accounting for its quality 0.1 ~ 1%, enzymolysis 4 ~ 10h at 37 ~ 45 DEG C respectively, obtain Radix Puerariae secondary enzymolysis liquid and the mixed plant secondary enzymolysis liquid except Radix Puerariae；
(3) one time fermentation: add the glucose in above-mentioned raw materials in described Radix Puerariae secondary enzymolysis liquid, the brown sugar (Saccharum Sinensis Roxb.) in above-mentioned raw materials is added in described mixed plant secondary enzymolysis liquid except Radix Puerariae, adding water, stirring to sugar is completely dissolved, then is added to 0.1 × 10 respectively9~1×109The leaven of CFU/L, reaches 3.5 ~ 4 at 37 ~ 40 DEG C of bottom fermentations to material liquid pH value, obtains Radix Puerariae fermentation liquid and the mixed plant fermentation liquid except Radix Puerariae；
Described leaven is the combination strain being made up of two or three in Lactobacillus bulgaricus, lactobacillus casei, streptococcus thermophilus, bacillus bifidus, Lactobacillus plantarum, lactobacillus rhamnosus, bacillus acidophilus；
(4) ferment in second time: the Radix Puerariae fermentation liquid respectively step (3) obtained and the mixed plant fermentation liquid except Radix Puerariae are filtered processing, the Radix Puerariae fermentating liquid filtrate obtained and the mixed plant fermentating liquid filtrate except Radix Puerariae are respectively taken in right amount and mix according to a certain percentage, in mixed filtrate, adds 0.1 × 109~1×109The leaven of CFU/L, reaches 3 ~ 3.5 at 37 ~ 40 DEG C of bottom fermentations to material liquid pH value, obtains ferment in second time liquid；
Described leaven is the combination strain being made up of two or three in Lactobacillus bulgaricus, lactobacillus casei, streptococcus thermophilus, bacillus bifidus, Lactobacillus plantarum, lactobacillus rhamnosus, bacillus acidophilus；
(5) filter, ripening: be filtered described ferment in second time liquid processing, the filtrate obtained be placed at 4 DEG C ~ 10 DEG C ripening at low temperature 4 ~ 60 days, obtain ripening liquid；
(6) allotment: add other raw materials except plant raw material and sugar, stirring and dissolving in described ripening liquid, adds appropriate citric acid and glucose regulates sugar-acid ratio, moderate to mouthfeel；
(7) fill sterilizing: liquid deployed for step (6) is carried out fill, sealing at dust proof workshop with 0.45 μm of membrane filtration and by every bottle of 30ml specification, then at 80 ~ 120 DEG C sterilizing 20 ~ 30min, namely obtain hepatoprotective plant oral liquid product.
In the another kind of preparation method of hepatoprotective plant oral liquid of the present invention, it is preferred that technical scheme or technology is characterized in that
1. in step (2): be separately added in described Radix Puerariae homogenate and account for the amylase of its quality 0.2%, cellulase, it is separately added in described mixed plant homogenate and accounts for the pectase of its quality 0.1%, cellulase, enzymolysis 4h at 45 DEG C, obtains Radix Puerariae primary enzymolysis liquid and the mixed plant primary enzymolysis liquid except Radix Puerariae；In above two primary enzymolysis liquid, add the acid protease accounting for its quality 0.1%, enzymolysis 4h at 40 DEG C respectively, obtain Radix Puerariae secondary enzymolysis liquid and the mixed plant secondary enzymolysis liquid except Radix Puerariae；
2. in step (3): add the glucose in above-mentioned raw materials in described Radix Puerariae enzymolysis solution, adding the brown sugar (Saccharum Sinensis Roxb.) in above-mentioned raw materials, add water in described mixed plant enzymolysis solution except Radix Puerariae, stirring to sugar is completely dissolved, then is added to 1 × 10 respectively9The leaven of CFU/L, reaches 4 at 37 DEG C of bottom fermentations to material liquid pH value, obtains Radix Puerariae fermentation liquid and mixed plant fermentation liquid.
3. in step (3): described leaven is mass ratio is Lactobacillus bulgaricus: lactobacillus casei: streptococcus thermophilus=2:1.5:1.
4., in step (4): the Radix Puerariae fermentation liquid respectively step (3) obtained and the mixed plant fermentation liquid except Radix Puerariae are filtered processing, the Radix Puerariae fermentating liquid filtrate obtained and the mixed plant fermentating liquid filtrate except Radix Puerariae respectively taken appropriate and mix according to volume ratio 1:2 or 1:1.
5. in step (4): add 0.1 × 10 in mixed filtrate9The leaven of CFU/L, reaches 3.5 at 40 DEG C of bottom fermentations to material liquid pH value, obtains ferment in second time liquid.
6. in step (4): described leaven is mass ratio is bacillus bifidus: Lactobacillus plantarum: lactobacillus rhamnosus=2:2:1, or mass ratio is lactobacillus rhamnosus: the combination strain of bacillus acidophilus=2:1.
7. in step (5): be filtered described ferment in second time liquid processing, be placed at 10 DEG C by the filtrate obtained ripening at low temperature 5 days, obtain ripening liquid.
8. in step (7) a metallic: liquid deployed for step (6) is carried out fill, sealing at dust proof workshop with 0.45 μm of membrane filtration and by every bottle of 30ml specification, then at 85 DEG C sterilizing 30min, namely obtain hepatoprotective plant oral liquid product.
Compared with prior art, the present invention passes through the plant material of preferred integration of edible and medicinal herbs and multiple fruit and vegerable, as: Fructus Crataegi, Fructus Jujubae, Semen phaseoli radiati, Pericarpium Citri Reticulatae, Radix Puerariae, Hericium erinaceus (Bull. Ex Fr.) Pers., Lentinus Edodes, Fructus Musae, pears, Fructus Lycopersici esculenti, Fructus Citri junoris, fresh Rhizoma Nelumbinis, Herba Apii graveolentis etc., optimized proportioning gives full play to the synergism of each raw material, reaches significant relieving alcoholism and protecting the liver effect.By adopting preparation method of the present invention, by the active component in raw material, such as puerarin, polysaccharide etc., discharged better than conventional water extraction method, and the hepatoprotective plant oral liquid taste perfume (or spice) prepared is felt well, overcome the bitter taste of the oral liquid adopting prior art to prepare, the especially bitterness of Radix Puerariae.Hepatoprotective plant oral liquid of the present invention not only has liver-protecting and alcoholism-relieving effect significantly, and is prone to drink, and has no side effect, and can meet the liver-protecting and alcoholism-relieving demand of normal person being not desired to take medicine.
Detailed description of the invention
The concrete technical scheme of the present invention is further described, in order to those skilled in the art is further understood that the present invention, and does not constitute the restriction to its right below in conjunction with embodiment.
Embodiment 1, a kind of hepatoprotective plant oral liquid, primary raw material and the weight proportion thereof of making this oral liquid include:
Fructus Crataegi 1；Fructus Jujubae 1；Semen phaseoli radiati 1；Pericarpium Citri Reticulatae 0.5；
Radix Puerariae 5；Glucose 2；Brown sugar (Saccharum Sinensis Roxb.) 3；Water 65.
Embodiment 2, a kind of hepatoprotective plant oral liquid, primary raw material and the weight proportion thereof of making this oral liquid include:
Fructus Crataegi 10；Fructus Jujubae 10；Semen phaseoli radiati 10；Pericarpium Citri Reticulatae 5；
Radix Puerariae 15；Glucose 8；Brown sugar (Saccharum Sinensis Roxb.) 12；Water 85.
Embodiment 3, a kind of hepatoprotective plant oral liquid, primary raw material and the weight proportion thereof of making this oral liquid include:
Fructus Crataegi 5；Fructus Jujubae 5；Semen phaseoli radiati 5；Pericarpium Citri Reticulatae 2.5；
Radix Puerariae 10；Glucose 6；Brown sugar (Saccharum Sinensis Roxb.) 8；Water 75.
Embodiment 4, a kind of hepatoprotective plant oral liquid, primary raw material and the weight proportion thereof of making this oral liquid include:
Fructus Crataegi 2；Fructus Jujubae 2；Semen phaseoli radiati 2；Pericarpium Citri Reticulatae 1；
Radix Puerariae 10；Glucose 4；Brown sugar (Saccharum Sinensis Roxb.) 6；Water 73.
Embodiment 5, a kind of hepatoprotective plant oral liquid described in embodiment 14 any one, the raw material making this oral liquid also includes the edible fungi of following weight proportion: Hericium erinaceus (Bull. Ex Fr.) Pers. 5；Lentinus Edodes 5.
Embodiment 6, a kind of hepatoprotective plant oral liquid described in embodiment 14 any one, the raw material making this oral liquid also includes the edible fungi of following weight proportion: Hericium erinaceus (Bull. Ex Fr.) Pers. 15；Lentinus Edodes 10.
Embodiment 7, a kind of hepatoprotective plant oral liquid described in embodiment 14 any one, the raw material making this oral liquid also includes the edible fungi of following weight proportion: Hericium erinaceus (Bull. Ex Fr.) Pers. 6；Lentinus Edodes 6.
Embodiment 8, a kind of hepatoprotective plant oral liquid described in embodiment 17 any one, the raw material making this oral liquid also includes one or more fruit and vegerable of following weight proportion: Fructus Musae 1；Pears 1；Fructus Lycopersici esculenti 1；Fructus Citri junoris 1；Fresh Rhizoma Nelumbinis 1；Herba Apii graveolentis 1.
Embodiment 9, a kind of hepatoprotective plant oral liquid described in embodiment 17 any one, the raw material making this oral liquid also includes one or more fruit and vegerable of following weight proportion: Fructus Musae 5；Pears 5；Fructus Lycopersici esculenti 5；Fructus Citri junoris 5；Fresh Rhizoma Nelumbinis 5；Herba Apii graveolentis 5.
Embodiment 10, a kind of hepatoprotective plant oral liquid described in embodiment 17 any one, the raw material making this oral liquid also includes one or more fruit and vegerable of following weight proportion: Fructus Musae 2；Pears 2；Fructus Lycopersici esculenti 2；Fructus Citri junoris 2；Fresh Rhizoma Nelumbinis 2；Herba Apii graveolentis 2.
Embodiment 11, a kind of hepatoprotective plant oral liquid described in embodiment 1 10 any one, the raw material making this oral liquid also includes vitamin B, vitamin C, make the content of vitamin B in described hepatoprotective plant oral liquid reach 0.05mg/100g, make ascorbic content in described hepatoprotective plant oral liquid reach 10mg/100g.
Embodiment 12, a kind of hepatoprotective plant oral liquid described in embodiment 1 10 any one, the raw material making this oral liquid also includes vitamin B, vitamin C, make the content of vitamin B in described hepatoprotective plant oral liquid reach 0.2mg/100g, make ascorbic content in described hepatoprotective plant oral liquid reach 50mg/100g.
Embodiment 13, a kind of hepatoprotective plant oral liquid described in embodiment 1 10 any one, the raw material making this oral liquid also includes vitamin B, vitamin C, make the content of vitamin B in described hepatoprotective plant oral liquid reach 0.1mg/100g, make ascorbic content in described hepatoprotective plant oral liquid reach 100mg/100g.
Embodiment 14, a kind of hepatoprotective plant oral liquid described in embodiment 1 13 any one, make one or more aminoacid that the raw material of this oral liquid can also include in glycine, arginine, leucine, isoleucine, make the content of described hepatoprotective plant Amino Acids in Oral Liquid reach 50mg/100g.
Embodiment 15, a kind of hepatoprotective plant oral liquid described in embodiment 1 13 any one, make one or more aminoacid that the raw material of this oral liquid can also include in glycine, arginine, leucine, isoleucine, make the content of described hepatoprotective plant Amino Acids in Oral Liquid reach 500mg/100g.
Embodiment 16, a kind of hepatoprotective plant oral liquid described in embodiment 1 13 any one, make one or more aminoacid that the raw material of this oral liquid can also include in glycine, arginine, leucine, isoleucine, make the content of described hepatoprotective plant Amino Acids in Oral Liquid reach 100mg/100g.
Embodiment 17, the preparation method of a kind of hepatoprotective plant oral liquid as described in embodiment 1-16 any one, it comprises the steps:
(1) Feedstock treating: clean and avoid Radix Puerariae to soak in water the plant raw material in above-mentioned raw materials with water, puts into refiner and adds water homogenate than 1:4 by material water quality, obtain homogenate after draining；
(2) enzymolysis: be separately added in described homogenate and respectively account for the amylase of its quality 0.1%, pectase and cellulase, enzymolysis 4h at 37 DEG C, obtain primary enzymolysis liquid；In primary enzymolysis liquid, add the acid protease accounting for its quality 0.1 ~ 1%, enzymolysis 4h at 37 DEG C, obtain secondary enzymolysis liquid；
(3) fermentation: adding the glucose in above-mentioned raw materials and brown sugar (Saccharum Sinensis Roxb.) in described enzymolysis solution, add water, stirring to sugar is completely dissolved, then is added to 0.1 × 109The leaven of CFU/L, reaches 3 at 37 DEG C of bottom fermentations to material liquid pH value, obtains fermentation liquid；
Described leaven is the combination strain being made up of two or three in Lactobacillus bulgaricus, lactobacillus casei, streptococcus thermophilus, bacillus bifidus, Lactobacillus plantarum, lactobacillus rhamnosus, bacillus acidophilus；
(4) filter, ripening: be filtered described fermentation liquid processing, filtrate be placed at 4 DEG C ripening at low temperature 4 days, obtain ripening liquid；
(5) allotment: add other raw materials except plant raw material and sugar, stirring and dissolving in described ripening liquid, adds appropriate citric acid and glucose regulates sugar-acid ratio, moderate to mouthfeel；
(6) fill sterilizing: liquid deployed in step (5) is carried out fill, sealing at dust proof workshop with 0.45 μm of membrane filtration and by every bottle of 30ml specification, then at 80 DEG C sterilizing 20min, namely obtain hepatoprotective plant oral liquid product.
Embodiment 18, the preparation method of a kind of hepatoprotective plant oral liquid as described in embodiment 1-16 any one, it comprises the steps:
(1) Feedstock treating: clean and avoid Radix Puerariae to soak in water the plant raw material in above-mentioned raw materials with water, puts into refiner and adds water homogenate than 1:4 by material water quality, obtain homogenate after draining；
(2) enzymolysis: be separately added in described homogenate and respectively account for the amylase of its quality 1%, pectase and cellulase, enzymolysis 16h at 45 DEG C, obtain primary enzymolysis liquid；In primary enzymolysis liquid, add the acid protease accounting for its quality 1%, enzymolysis 10h at 45 DEG C, obtain secondary enzymolysis liquid；
(3) fermentation: adding the glucose in above-mentioned raw materials and brown sugar (Saccharum Sinensis Roxb.) in described enzymolysis solution, add water, stirring to sugar is completely dissolved, then is added to 1 × 109The leaven of CFU/L, reaches 4 at 40 DEG C of bottom fermentations to material liquid pH value, obtains fermentation liquid；
Described leaven is the combination strain being made up of two or three in Lactobacillus bulgaricus, lactobacillus casei, streptococcus thermophilus, bacillus bifidus, Lactobacillus plantarum, lactobacillus rhamnosus, bacillus acidophilus；
(4) filter, ripening: be filtered described fermentation liquid processing, filtrate be placed at 10 DEG C ripening at low temperature 60 days, obtain ripening liquid；
(5) allotment: add other raw materials except plant raw material and sugar, stirring and dissolving in described ripening liquid, adds appropriate citric acid and glucose regulates sugar-acid ratio, moderate to mouthfeel；
(6) fill sterilizing: liquid deployed in step (5) is carried out fill, sealing at dust proof workshop with 0.45 μm of membrane filtration and by every bottle of 30ml specification, then at 120 DEG C sterilizing 30min, namely obtain hepatoprotective plant oral liquid product.
Embodiment 19, the preparation method of a kind of hepatoprotective plant oral liquid as described in embodiment 1-16 any one, it comprises the steps:
(1) Feedstock treating: clean and avoid Radix Puerariae to soak in water the plant raw material in above-mentioned raw materials with water, puts into refiner and adds water homogenate than 1:4 by material water quality, obtain homogenate after draining；
(2) enzymolysis: be separately added in described homogenate and respectively account for the amylase of its quality 0.5%, pectase and cellulase, enzymolysis 10h at 40 DEG C, obtain primary enzymolysis liquid；In primary enzymolysis liquid, add the acid protease accounting for its quality 0.5%, enzymolysis 7h at 40 DEG C, obtain secondary enzymolysis liquid；
(3) fermentation: adding the glucose in above-mentioned raw materials and brown sugar (Saccharum Sinensis Roxb.) in described enzymolysis solution, add water, stirring to sugar is completely dissolved, then is added to 0.5 × 109The leaven of CFU/L, reaches 3.5 at 40 DEG C of bottom fermentations to material liquid pH value, obtains fermentation liquid；
Described leaven is the combination strain being made up of two or three in Lactobacillus bulgaricus, lactobacillus casei, streptococcus thermophilus, bacillus bifidus, Lactobacillus plantarum, lactobacillus rhamnosus, bacillus acidophilus；
(4) filter, ripening: be filtered described fermentation liquid processing, filtrate be placed at 6 DEG C ripening at low temperature 32 days, obtain ripening liquid；
(5) allotment: add other raw materials except plant raw material and sugar, stirring and dissolving in described ripening liquid, adds appropriate citric acid and glucose regulates sugar-acid ratio, moderate to mouthfeel；
(6) fill sterilizing: liquid deployed in step (5) is carried out fill, sealing at dust proof workshop with 0.45 μm of membrane filtration and by every bottle of 30ml specification, then at 100 DEG C sterilizing 25min, namely obtain hepatoprotective plant oral liquid product.
Embodiment 20, the preparation method of a kind of hepatoprotective plant oral liquid as described in embodiment 1-16 any one, it comprises the steps:
(1) Feedstock treating: clean and avoid Radix Puerariae to soak in water the plant raw material in above-mentioned raw materials with water, puts into refiner and adds water homogenate than 1:4 by material water quality, obtain homogenate after draining；
(2) enzymolysis: be separately added in described homogenate and respectively account for the amylase of its quality 0.1%, pectase and cellulase, enzymolysis 4h at 45 DEG C, obtain primary enzymolysis liquid；In primary enzymolysis liquid, add the acid protease accounting for its quality 0.1%, enzymolysis 10h at 40 DEG C, obtain secondary enzymolysis liquid；
(3) fermentation: adding the glucose in above-mentioned raw materials and brown sugar (Saccharum Sinensis Roxb.) in described enzymolysis solution, add water, stirring to sugar is completely dissolved, then is added to 1 × 109The leaven of CFU/L, reaches 3.5 at 37 DEG C of bottom fermentations to material liquid pH value, obtains fermentation liquid；
Described leaven is the combination strain being made up of two or three in Lactobacillus bulgaricus, lactobacillus casei, streptococcus thermophilus, bacillus bifidus, Lactobacillus plantarum, lactobacillus rhamnosus, bacillus acidophilus；
(4) filter, ripening: be filtered described fermentation liquid processing, filtrate be placed at 6 DEG C ripening at low temperature 10 days, obtain ripening liquid；
(5) allotment: add other raw materials except plant raw material and sugar, stirring and dissolving in described ripening liquid, adds appropriate citric acid and glucose regulates sugar-acid ratio, moderate to mouthfeel；
(6) fill sterilizing: liquid deployed in step (5) is carried out fill, sealing at dust proof workshop with 0.45 μm of membrane filtration and by every bottle of 30ml specification, then at 85 DEG C sterilizing 30min, namely obtain hepatoprotective plant oral liquid product.
Embodiment 21, in the step (3) of the preparation method of a kind of hepatoprotective plant oral liquid described in embodiment 17 19 any one: described leaven is mass ratio is Lactobacillus bulgaricus: lactobacillus casei: streptococcus thermophilus=2:1.5:1, combination strain.All the other are identical.
Embodiment 22, in the step (3) of the preparation method of a kind of hepatoprotective plant oral liquid described in embodiment 20: described leaven is mass ratio is Lactobacillus bulgaricus: lactobacillus casei: streptococcus thermophilus=2:1.5:1, combination strain.All the other are identical.
Embodiment 23, in the step (3) of the preparation method of a kind of hepatoprotective plant oral liquid described in embodiment 17 20 any one: described leaven is mass ratio is bacillus bifidus: Lactobacillus plantarum: lactobacillus rhamnosus=2:2:1 combines strain.All the other are identical.
Embodiment 24, the preparation method of a kind of hepatoprotective plant oral liquid as described in embodiment 1-16 any one, it comprises the steps:
(1) Feedstock treating: clean and avoid Radix Puerariae to soak in water the plant raw material in above-mentioned raw materials with water, after draining, Radix Puerariae and the other plant class raw material except Radix Puerariae are separately used refiner homogenate, all add water homogenate than 1:4 by material water quality during homogenate, respectively obtain Radix Puerariae homogenate and the mixed plant homogenate except Radix Puerariae；
(2) enzymolysis: be separately added in described Radix Puerariae homogenate and respectively account for the amylase of its quality 0.1%, cellulase, it is separately added in the described mixed plant homogenate except Radix Puerariae and respectively accounts for the pectase of its quality 0.1%, cellulase, enzymolysis 4h at 37 DEG C, obtains Radix Puerariae primary enzymolysis liquid and the mixed plant primary enzymolysis liquid except Radix Puerariae；In above two primary enzymolysis liquid, add the acid protease accounting for its quality 0.1%, enzymolysis 4h at 37 DEG C respectively, obtain Radix Puerariae secondary enzymolysis liquid and the mixed plant secondary enzymolysis liquid except Radix Puerariae；
(3) one time fermentation: add the glucose in above-mentioned raw materials in described Radix Puerariae secondary enzymolysis liquid, the brown sugar (Saccharum Sinensis Roxb.) in above-mentioned raw materials is added in described mixed plant secondary enzymolysis liquid except Radix Puerariae, adding water, stirring to sugar is completely dissolved, then is added to 0.1 × 10 respectively9The leaven of CFU/L, reaches 3.5 at 37 DEG C of bottom fermentations to material liquid pH value, obtains Radix Puerariae fermentation liquid and the mixed plant fermentation liquid except Radix Puerariae；
Described leaven is the combination strain being made up of two or three in Lactobacillus bulgaricus, lactobacillus casei, streptococcus thermophilus, bacillus bifidus, Lactobacillus plantarum, lactobacillus rhamnosus, bacillus acidophilus；
(4) ferment in second time: the Radix Puerariae fermentation liquid respectively step (3) obtained and the mixed plant fermentation liquid except Radix Puerariae are filtered processing, the Radix Puerariae fermentating liquid filtrate obtained and the mixed plant fermentating liquid filtrate except Radix Puerariae are respectively taken in right amount and mix according to a certain percentage, in mixed filtrate, adds 0.1 × 109The leaven of CFU/L, reaches 3 at 37 DEG C of bottom fermentations to material liquid pH value, obtains ferment in second time liquid；
Described leaven is the combination strain being made up of two or three in Lactobacillus bulgaricus, lactobacillus casei, streptococcus thermophilus, bacillus bifidus, Lactobacillus plantarum, lactobacillus rhamnosus, bacillus acidophilus；
(5) filter, ripening: be filtered described ferment in second time liquid processing, be placed at 4 DEG C by the filtrate obtained ripening at low temperature 4 days, obtain ripening liquid；
(6) allotment: add other raw materials except plant raw material and sugar, stirring and dissolving in described ripening liquid, adds appropriate citric acid and glucose regulates sugar-acid ratio, moderate to mouthfeel；
(7) fill sterilizing: liquid deployed for step (6) is carried out fill, sealing at dust proof workshop with 0.45 μm of membrane filtration and by every bottle of 30ml specification, then at 80 DEG C sterilizing 20min, namely obtain hepatoprotective plant oral liquid product.
Embodiment 25, the preparation method of a kind of hepatoprotective plant oral liquid as described in embodiment 1-16 any one, it comprises the steps:
(1) Feedstock treating: clean and avoid Radix Puerariae to soak in water the plant raw material in above-mentioned raw materials with water, after draining, Radix Puerariae and the other plant class raw material except Radix Puerariae are separately used refiner homogenate, all add water homogenate than 1:4 by material water quality during homogenate, respectively obtain Radix Puerariae homogenate and the mixed plant homogenate except Radix Puerariae；
(2) enzymolysis: be separately added in described Radix Puerariae homogenate and respectively account for the amylase of its quality 1%, cellulase, it is separately added in the described mixed plant homogenate except Radix Puerariae and respectively accounts for the pectase of its quality 1%, cellulase, enzymolysis 16h at 45 DEG C, obtains Radix Puerariae primary enzymolysis liquid and the mixed plant primary enzymolysis liquid except Radix Puerariae；In above two primary enzymolysis liquid, add the acid protease accounting for its quality 1%, enzymolysis 10h at 45 DEG C respectively, obtain Radix Puerariae secondary enzymolysis liquid and the mixed plant secondary enzymolysis liquid except Radix Puerariae；
(3) one time fermentation: add the glucose in above-mentioned raw materials in described Radix Puerariae secondary enzymolysis liquid, the brown sugar (Saccharum Sinensis Roxb.) in above-mentioned raw materials is added in described mixed plant secondary enzymolysis liquid except Radix Puerariae, adding water, stirring to sugar is completely dissolved, then is added to 1 × 10 respectively9The leaven of CFU/L, reaches 4 at 40 DEG C of bottom fermentations to material liquid pH value, obtains Radix Puerariae fermentation liquid and the mixed plant fermentation liquid except Radix Puerariae；
Described leaven is the combination strain being made up of two or three in Lactobacillus bulgaricus, lactobacillus casei, streptococcus thermophilus, bacillus bifidus, Lactobacillus plantarum, lactobacillus rhamnosus, bacillus acidophilus；
(4) ferment in second time: the Radix Puerariae fermentation liquid respectively step (3) obtained and the mixed plant fermentation liquid except Radix Puerariae are filtered processing, the Radix Puerariae fermentating liquid filtrate obtained and the mixed plant fermentating liquid filtrate except Radix Puerariae are respectively taken in right amount and mix according to a certain percentage, in mixed filtrate, adds 0.1 × 109The leaven of CFU/L, reaches 3 at 37 DEG C of bottom fermentations to material liquid pH value, obtains ferment in second time liquid；
Described leaven is the combination strain being made up of two or three in Lactobacillus bulgaricus, lactobacillus casei, streptococcus thermophilus, bacillus bifidus, Lactobacillus plantarum, lactobacillus rhamnosus, bacillus acidophilus；
(5) filter, ripening: be filtered described ferment in second time liquid processing, be placed at 10 DEG C by the filtrate obtained ripening at low temperature 60 days, obtain ripening liquid；
(6) allotment: add other raw materials except plant raw material and sugar, stirring and dissolving in described ripening liquid, adds appropriate citric acid and glucose regulates sugar-acid ratio, moderate to mouthfeel；
(7) fill sterilizing: liquid deployed for step (6) is carried out fill, sealing at dust proof workshop with 0.45 μm of membrane filtration and by every bottle of 30ml specification, then at 120 DEG C sterilizing 30min, namely obtain hepatoprotective plant oral liquid product.
Embodiment 26, the preparation method of a kind of hepatoprotective plant oral liquid as described in embodiment 1-16 any one, it comprises the steps:
(1) Feedstock treating: clean and avoid Radix Puerariae to soak in water the plant raw material in above-mentioned raw materials with water, after draining, Radix Puerariae and the other plant class raw material except Radix Puerariae are separately used refiner homogenate, all add water homogenate than 1:4 by material water quality during homogenate, respectively obtain Radix Puerariae homogenate and the mixed plant homogenate except Radix Puerariae；
(2) enzymolysis: be separately added in described Radix Puerariae homogenate and respectively account for the amylase of its quality 0.5%, cellulase, it is separately added in the described mixed plant homogenate except Radix Puerariae and respectively accounts for the pectase of its quality 0.5%, cellulase, enzymolysis 10h at 42 DEG C, obtains Radix Puerariae primary enzymolysis liquid and the mixed plant primary enzymolysis liquid except Radix Puerariae；In above two primary enzymolysis liquid, add the acid protease accounting for its quality 0.5%, enzymolysis 4h at 40 DEG C respectively, obtain Radix Puerariae secondary enzymolysis liquid and the mixed plant secondary enzymolysis liquid except Radix Puerariae；
(3) one time fermentation: add the glucose in above-mentioned raw materials in described Radix Puerariae secondary enzymolysis liquid, the brown sugar (Saccharum Sinensis Roxb.) in above-mentioned raw materials is added in described mixed plant secondary enzymolysis liquid except Radix Puerariae, adding water, stirring to sugar is completely dissolved, then is added to 0.5 × 10 respectively9The leaven of CFU/L, reaches 3.5 at 37 DEG C of bottom fermentations to material liquid pH value, obtains Radix Puerariae fermentation liquid and the mixed plant fermentation liquid except Radix Puerariae；
Described leaven is the combination strain being made up of two or three in Lactobacillus bulgaricus, lactobacillus casei, streptococcus thermophilus, bacillus bifidus, Lactobacillus plantarum, lactobacillus rhamnosus, bacillus acidophilus；
(4) ferment in second time: the Radix Puerariae fermentation liquid respectively step (3) obtained and the mixed plant fermentation liquid except Radix Puerariae are filtered processing, the Radix Puerariae fermentating liquid filtrate obtained and the mixed plant fermentating liquid filtrate except Radix Puerariae are respectively taken in right amount and mix according to a certain percentage, in mixed filtrate, adds 0.1 × 109The leaven of CFU/L, reaches 3 at 37 DEG C of bottom fermentations to material liquid pH value, obtains ferment in second time liquid；
Described leaven is the combination strain being made up of two or three in Lactobacillus bulgaricus, lactobacillus casei, streptococcus thermophilus, bacillus bifidus, Lactobacillus plantarum, lactobacillus rhamnosus, bacillus acidophilus；
(5) filter, ripening: be filtered described ferment in second time liquid processing, be placed at 6 DEG C by the filtrate obtained ripening at low temperature 32 days, obtain ripening liquid；
(6) allotment: add other raw materials except plant raw material and sugar, stirring and dissolving in described ripening liquid, adds appropriate citric acid and glucose regulates sugar-acid ratio, moderate to mouthfeel；
(7) fill sterilizing: liquid deployed for step (6) is carried out fill, sealing at dust proof workshop with 0.45 μm of membrane filtration and by every bottle of 30ml specification, then at 100 DEG C sterilizing 25min, namely obtain hepatoprotective plant oral liquid product.
Embodiment 27, the preparation method of a kind of hepatoprotective plant oral liquid as described in embodiment 1-16 any one, it comprises the steps:
(1) Feedstock treating: clean and avoid Radix Puerariae to soak in water the plant raw material in above-mentioned raw materials with water, after draining, Radix Puerariae and the other plant class raw material except Radix Puerariae are separately used refiner homogenate, all add water homogenate than 1:4 by material water quality during homogenate, respectively obtain Radix Puerariae homogenate and the mixed plant homogenate except Radix Puerariae；
(2) enzymolysis: be separately added in described Radix Puerariae homogenate and respectively account for the amylase of its quality 0.2%, cellulase, it is separately added in the described mixed plant homogenate except Radix Puerariae and respectively accounts for the pectase of its quality 0.1%, cellulase, enzymolysis 4h at 45 DEG C, obtains Radix Puerariae primary enzymolysis liquid and the mixed plant primary enzymolysis liquid except Radix Puerariae；In above two primary enzymolysis liquid, add the acid protease accounting for its quality 0.1%, enzymolysis 4h at 40 DEG C respectively, obtain Radix Puerariae secondary enzymolysis liquid and the mixed plant secondary enzymolysis liquid except Radix Puerariae；
(3) one time fermentation: add the glucose in above-mentioned raw materials in described Radix Puerariae secondary enzymolysis liquid, the brown sugar (Saccharum Sinensis Roxb.) in above-mentioned raw materials is added in described mixed plant secondary enzymolysis liquid except Radix Puerariae, adding water, stirring to sugar is completely dissolved, then is added to 1 × 10 respectively9The leaven of CFU/L, reaches 4 at 37 DEG C of bottom fermentations to material liquid pH value, obtains Radix Puerariae fermentation liquid and the mixed plant fermentation liquid except Radix Puerariae；
Described leaven is the combination strain being made up of two or three in Lactobacillus bulgaricus, lactobacillus casei, streptococcus thermophilus, bacillus bifidus, Lactobacillus plantarum, lactobacillus rhamnosus, bacillus acidophilus；
(4) ferment in second time: the Radix Puerariae fermentation liquid respectively step (3) obtained and the mixed plant fermentation liquid except Radix Puerariae are filtered processing, the Radix Puerariae fermentating liquid filtrate obtained and the mixed plant fermentating liquid filtrate except Radix Puerariae are respectively taken in right amount and mix according to a certain percentage, in mixed filtrate, adds 0.1 × 109The leaven of CFU/L, reaches 3 at 37 DEG C of bottom fermentations to material liquid pH value, obtains ferment in second time liquid；
Described leaven is the combination strain being made up of two or three in Lactobacillus bulgaricus, lactobacillus casei, streptococcus thermophilus, bacillus bifidus, Lactobacillus plantarum, lactobacillus rhamnosus, bacillus acidophilus；
(5) filter, ripening: be filtered described ferment in second time liquid processing, be placed at 10 DEG C by the filtrate obtained ripening at low temperature 5 days, obtain ripening liquid；
(6) allotment: add other raw materials except plant raw material and sugar, stirring and dissolving in described ripening liquid, adds appropriate citric acid and glucose regulates sugar-acid ratio, moderate to mouthfeel；
(7) fill sterilizing: liquid deployed for step (6) is carried out fill, sealing at dust proof workshop with 0.45 μm of membrane filtration and by every bottle of 30ml specification, then at 85 DEG C sterilizing 30min, namely obtain hepatoprotective plant oral liquid product.
Embodiment 28, in the step (3) of the preparation method of a kind of hepatoprotective plant oral liquid described in embodiment 24 26 any one: described leaven is mass ratio is Lactobacillus bulgaricus: lactobacillus casei: the combination strain of streptococcus thermophilus=2:1.5:1.All the other are identical.
Embodiment 29, in the step (3) of the preparation method of a kind of hepatoprotective plant oral liquid described in embodiment 27: described leaven is mass ratio is Lactobacillus bulgaricus: lactobacillus casei: the combination strain of streptococcus thermophilus=2:1.5:1.All the other are identical.
Embodiment 30, in the step (4) of the preparation method of a kind of hepatoprotective plant oral liquid described in embodiment 24 29 any one: the Radix Puerariae fermentation liquid respectively step (3) obtained and the mixed plant fermentation liquid except Radix Puerariae are filtered processing, the Radix Puerariae fermentating liquid filtrate obtained and the mixed plant fermentating liquid filtrate except Radix Puerariae respectively taken appropriate and mix according to volume ratio 1:2.All the other are identical.
Embodiment 31, in the step (4) of the preparation method of a kind of hepatoprotective plant oral liquid described in embodiment 24 29 any one: the Radix Puerariae fermentation liquid respectively step (3) obtained and the mixed plant fermentation liquid except Radix Puerariae are filtered processing, the Radix Puerariae fermentating liquid filtrate obtained and the mixed plant fermentating liquid filtrate except Radix Puerariae respectively taken appropriate and mix according to volume ratio 1:1.All the other are identical.
Embodiment 32, in the step (4) of the preparation method of a kind of hepatoprotective plant oral liquid described in embodiment 24 31 any one: described leaven is mass ratio is Lactobacillus bulgaricus: lactobacillus casei: streptococcus thermophilus=2:2:1, combination strain.All the other are identical.
Embodiment 33, in the step (4) of the preparation method of a kind of hepatoprotective plant oral liquid described in embodiment 24 28 any one: described leaven is mass ratio is lactobacillus rhamnosus: the combination strain of bacillus acidophilus=2:1.All the other are identical.
Embodiment 34, in the step (4) of the preparation method of a kind of hepatoprotective plant oral liquid described in embodiment 29: described leaven is mass ratio is lactobacillus rhamnosus: the combination strain of bacillus acidophilus=2:1.All the other are identical.
Embodiment 35, in the step (4) of the preparation method of a kind of hepatoprotective plant oral liquid described in embodiment 30 31 any one: described leaven is mass ratio is lactobacillus rhamnosus: the combination strain of bacillus acidophilus=2:1.All the other are identical.
Adopt the present invention to carry out animal experiment in order to further illustrate effect of the present invention, probe into the impact that formula and preparation method effect on hepatoprotective plant oral liquid produces simultaneously.
One, test packet and gavage
Test sets Normal group, model control group, given the test agent group (formula and/or preparation method are different), totally 8 groups.Take 48 healthy adult mices, be randomly divided into 8 groups, often group 6.
1. given the test agent
1.1 given the test agent 1
(1) formula and proportioning: hepatoprotective plant oral liquid, raw material and weight proportion thereof following (unit: kg):
Fructus Crataegi 1；Fructus Jujubae 1；Semen phaseoli radiati 1；
Pericarpium Citri Reticulatae 0.5；Glucose 2；Brown sugar (Saccharum Sinensis Roxb.) 3；
Water is appropriate, final volume 50L.
(2) preparation method: adopt the method described in embodiment 22, obtain hepatoprotective plant oral liquid A, i.e. given the test agent 1.
1.2 given the test agent 2
(1) formula and proportioning: hepatoprotective plant oral liquid, raw material and weight proportion thereof following (unit: kg):
Fructus Crataegi 1；Fructus Jujubae 1；Semen phaseoli radiati 1；Pericarpium Citri Reticulatae 0.5；
Radix Puerariae 5；Glucose 2；Brown sugar (Saccharum Sinensis Roxb.) 3；
Water is appropriate, final volume 50L.
(2) preparation method: adopt the method described in embodiment 22, obtain hepatoprotective plant oral liquid B, i.e. given the test agent 2.
1.3 given the test agent 3
(1) formula and proportioning: hepatoprotective plant oral liquid, raw material and weight proportion thereof following (unit: kg):
Fructus Crataegi 1；Fructus Jujubae 1；Semen phaseoli radiati 1；Pericarpium Citri Reticulatae 0.2；Radix Puerariae 3；
Hericium erinaceus (Bull. Ex Fr.) Pers. 3；Lentinus Edodes 3；Glucose 2；Brown sugar (Saccharum Sinensis Roxb.) 3；
Water is appropriate, final volume 50L.
(2) preparation method: adopt the method described in embodiment 22, obtain hepatoprotective plant oral liquid C, i.e. given the test agent 3.
1.4 given the test agent 4
(1) formula and proportioning: hepatoprotective plant oral liquid, raw material and weight proportion thereof following (unit: kg):
Fructus Crataegi 1；Fructus Jujubae 1；Semen phaseoli radiati 1；Pericarpium Citri Reticulatae 0.2；Radix Puerariae 3；
Hericium erinaceus (Bull. Ex Fr.) Pers. 3；Lentinus Edodes 3；Fructus Musae 1；Pears 1；Fructus Lycopersici esculenti 1；
Fructus Citri junoris 1；Fresh Rhizoma Nelumbinis 1；Herba Apii graveolentis 1；Glucose 2；Brown sugar (Saccharum Sinensis Roxb.) 3；
Water is appropriate, final volume 50L.
(2) preparation method: adopt the method described in embodiment 34, obtain hepatoprotective plant oral liquid D, i.e. given the test agent 4.
1.5 given the test agent 5
(1) formula and proportioning: hepatoprotective plant oral liquid, raw material and weight proportion thereof following (unit: kg):
Fructus Crataegi 1；Fructus Jujubae 1；Semen phaseoli radiati 1；Pericarpium Citri Reticulatae 0.2；Radix Puerariae 3；
Hericium erinaceus (Bull. Ex Fr.) Pers. 3；Lentinus Edodes 3；Fructus Musae 1；Pears 1；Fructus Lycopersici esculenti 1；
Fructus Citri junoris 1；Fresh Rhizoma Nelumbinis 1；Herba Apii graveolentis 1；Glucose 2；Brown sugar (Saccharum Sinensis Roxb.) 3；
Vitamin B appropriate (in the hepatoprotective plant oral liquid that guarantee prepares, vitamin B reaches 0.1mg/100g)；Vitamin C appropriate (in the hepatoprotective plant oral liquid that guarantee prepares, vitamin C reaches 100mg/100g)；
Water is appropriate, final volume 50L.
(2) preparation method: adopt the method described in embodiment 34, obtain hepatoprotective plant oral liquid E, i.e. given the test agent 5.
1.6 given the test agent 6
(1) formula and proportioning: hepatoprotective plant oral liquid, raw material and weight proportion thereof following (unit: kg):
Fructus Crataegi 1；Fructus Jujubae 1；Semen phaseoli radiati 1；Pericarpium Citri Reticulatae 0.2；Radix Puerariae 3；
Hericium erinaceus (Bull. Ex Fr.) Pers. 3；Lentinus Edodes 3；Fructus Musae 1；Pears 1；Fructus Lycopersici esculenti 1；
Fructus Citri junoris 1；Fresh Rhizoma Nelumbinis 1；Herba Apii graveolentis 1；Glucose 2；Brown sugar (Saccharum Sinensis Roxb.) 3；
Vitamin B appropriate (in the hepatoprotective plant oral liquid that guarantee prepares, vitamin B reaches 0.1mg/100g)；Vitamin C appropriate (in the hepatoprotective plant oral liquid that guarantee prepares, vitamin C reaches 100mg/100g)；
Compound vitamin appropriate (in the hepatoprotective plant oral liquid that guarantee prepares, compound vitamin content reaches 100mg/100g)；
Water is appropriate, final volume 50L.
(2) preparation method: adopt the method described in embodiment 34, obtain hepatoprotective plant oral liquid F, i.e. given the test agent 6.
2. mouse stomach experiment
The normal saline of Normal group gavage 0.1mL/10g every day body weight；52 degree of Chinese liquor of model control group gavage 0.1mL/10g every day body weight；After each given the test agent group elder generation's every day gavage 0.1mL/10g body weight (with physiological saline solution) each given the test agent, then the 52 of gavage 0.1mL/10g body weight degree of Chinese liquor.Continuous gavage 20 days, observes mice movable, records dead mouse situation.
Two, the impact on mouse liver malonaldehyde (MDA) level
Hepatoprotective plant oral liquid efficacy assessments damages the index MDA level of liver function for representative in each group of mouse liver, and in liver, MDA content is more high, illustrates that in liver, relevant enzyme and membranous system degree of injury are more high.
Above-mentioned experiment mice after gavage 20 days, overnight fast but can't help water, next day takes liver.Weigh each experiment mice liver 0.2g in mortar, grind with 2mLPBS and make homogenate, after 5000r/min is centrifugal, takes supernatant, detect liver organization MDA.In process of the test, dead mice is not tested.
The preparation of detection liquid: 0.6g thiobarbituricacidα-adds (about 5mL) 1MNaOH solution on a small quantity and dissolves, then is settled to 100mL with 20% trichloroacetic acid, namely obtains the thiobarbituricacidα--trichloroacetic acid detection liquid of 0.6%.
Detection method: detection liquid and the mixing of liver organization supernatant equal-volume, boiling water bath 15min, is then rapidly cooled to room temperature, and 12000r/min is centrifuged 10min, takes supernatant and surveys light absorption value at 532nm place.
After testing, in each group experiment mice liver, the testing result of MDA level (in absorbance OD532) is as shown in table 1:
MDA level in experiment mice liver respectively organized by table 1.
Note: *: compare P < 0.05, * * with Normal group: compare P < 0.001 with Normal group；
#: P < 0.05 is compared with model control group,##: P < 0.001 is compared with model control group.
Through statistical analysis it can be seen that the liver MDA level of model control group and Normal group exists pole significant difference (P < 0.001), it was shown that modeling success.
Compared with Normal group, the liver MDA level of given the test agent group 4 ~ 6 and Normal group there was no significant difference (P > 0.05)；The liver MDA level of given the test agent group 2,3 and Normal group has significant difference (P < 0.05)；Given the test agent group 1, the test group of the hepatoprotective plant oral liquid A namely applying raw material described in non-invention but adopt the preparation method of the present invention to prepare, its liver MDA level is significantly higher than the liver MDA level of Normal group, has pole significant difference (P < 0.001).
Compared with model control group, given the test agent group all can reduce liver MDA level, and all there is pole significant difference (P < 0.001) except given the test agent group 1 with the liver MDA level of model control group.Wherein, given the test agent group 5,6 liver MDA level relatively model control group declines the most obvious, namely drinks the test group of hepatoprotective plant oral liquid E and F of the present invention, practically drops to the liver MDA level of Normal group, illustrate that liver is protected completely；Next to that given the test agent group 4, namely drink the test group of hepatoprotective plant oral liquid D of the present invention；Given the test agent group 4 ~ 6 all adopts hepatoprotective plant oral liquid of the present invention, it was shown that the present invention has significant hepatoprotective effect.
By testing it can be seen that relieving alcoholism and protecting the liver effect of hepatoprotective plant oral liquid is had certain impact by the difference of formula and/or preparation method.Add Radix Puerariae, fruit and vegerable and supplementary appropriate trace element (vitamin and aminoacid) in the feed and advantageously reduce liver MDA level, hepatoprotective better；The formulation ratio of optimization can be obtained the significant hepatoprotective plant oral liquid of effect in conjunction with the preparation method of the present invention.
Claims (13)
1. a hepatoprotective plant oral liquid, it is characterised in that primary raw material and the weight proportion thereof of making this oral liquid include:
Fructus Crataegi 1 ~ 10；Fructus Jujubae 1 ~ 10；Semen phaseoli radiati 1 ~ 10；Pericarpium Citri Reticulatae 0.5 ~ 5；
Radix Puerariae 5 ~ 15；Glucose 2 ~ 8；Brown sugar (Saccharum Sinensis Roxb.) 3 ~ 12；Water 65 ~ 85.
2. hepatoprotective plant oral liquid according to claim 1, it is characterised in that the weight proportion of each raw material is: Fructus Crataegi 2；Fructus Jujubae 2；Semen phaseoli radiati 2；Pericarpium Citri Reticulatae 1；Radix Puerariae 10；Glucose 4；Brown sugar (Saccharum Sinensis Roxb.) 6；Water 73.
3. hepatoprotective plant oral liquid according to claim 1 and 2, it is characterised in that the raw material making this oral liquid also includes the edible fungi of following weight proportion: Hericium erinaceus (Bull. Ex Fr.) Pers. 5 ~ 15；Lentinus Edodes 5 ~ 10.
4. the hepatoprotective plant oral liquid in any of the one of claim 1 or 2 or 3, it is characterised in that the raw material making this oral liquid also includes one or more fruit and vegerable of following weight proportion: Fructus Musae 1 ~ 5；Pears 1 ~ 5；Fructus Lycopersici esculenti 1 ~ 5；Fructus Citri junoris 1 ~ 5；Fresh Rhizoma Nelumbinis 1 ~ 5；Herba Apii graveolentis 1 ~ 5.
5. the hepatoprotective plant oral liquid in any of the one of claim 14, it is characterized in that, the raw material making this oral liquid also includes vitamin B, vitamin C, make the content of vitamin B in described hepatoprotective plant oral liquid reach 0.05 ~ 0.2mg/100g, make ascorbic content in described hepatoprotective plant oral liquid reach 10 ~ 100mg/100g.
6. the hepatoprotective plant oral liquid in any of the one of claim 15, it is characterized in that, make one or more aminoacid that the raw material of this oral liquid also includes in glycine, arginine, leucine, isoleucine, make the content of described hepatoprotective plant Amino Acids in Oral Liquid reach 50 ~ 500mg/100g.
7. the preparation method of the hepatoprotective plant oral liquid as in any of the one of claim 16, it is characterised in that it comprises the steps:
(1) Feedstock treating: clean and avoid Radix Puerariae to soak in water the plant raw material in above-mentioned raw materials with water, puts into refiner and adds water homogenate than 1:4 by material water quality, obtain homogenate after draining；
(2) enzymolysis: be separately added in described homogenate and respectively account for the amylase of its quality 0.1 ~ 1%, pectase and cellulase, enzymolysis 4 ~ 16h at 37 ~ 45 DEG C, obtain primary enzymolysis liquid；In primary enzymolysis liquid, add the acid protease accounting for its quality 0.1 ~ 1%, enzymolysis 4 ~ 10h at 37 ~ 45 DEG C, obtain secondary enzymolysis liquid；
(3) fermentation: adding the glucose in above-mentioned raw materials and brown sugar (Saccharum Sinensis Roxb.) in described secondary enzymolysis liquid, add water, stirring to sugar is completely dissolved, then is added to 0.1 × 109~1×109The leaven of CFU/L, reaches 3 ~ 4 at 37 ~ 40 DEG C of bottom fermentations to material liquid pH value, obtains fermentation liquid；
Described leaven is the combination strain being made up of two or three in Lactobacillus bulgaricus, lactobacillus casei, streptococcus thermophilus, bacillus bifidus, Lactobacillus plantarum, lactobacillus rhamnosus, bacillus acidophilus；
(4) filter, ripening: be filtered described fermentation liquid processing, filtrate be placed at 4 DEG C ~ 10 DEG C ripening at low temperature 4 ~ 60 days, obtain ripening liquid；
(5) allotment: add other raw materials except plant raw material and sugar, stirring and dissolving in described ripening liquid, adds appropriate citric acid and glucose regulates sugar-acid ratio, moderate to mouthfeel；
(6) fill sterilizing: liquid deployed in step (5) is carried out fill, sealing at dust proof workshop with 0.45 μm of membrane filtration and by every bottle of 30ml specification, then at 80 ~ 120 DEG C sterilizing 20 ~ 30min, namely obtain hepatoprotective plant oral liquid product.
8. the preparation method of hepatoprotective plant oral liquid according to claim 7, it is characterized in that, in step (2), be separately added in described homogenate and respectively account for the amylase of its quality 0.1%, pectase and cellulase, enzymolysis 4h at 45 DEG C, obtains primary enzymolysis liquid；In primary enzymolysis liquid, add the acid protease accounting for its quality 0.1%, enzymolysis 4h at 40 DEG C, obtain secondary enzymolysis liquid.
9. the preparation method of hepatoprotective plant oral liquid according to claim 7, it is characterised in that in step (3), adds the sugar in above-mentioned raw materials and water in described secondary enzymolysis liquid, and stirring to sugar is completely dissolved, then is added to 1 × 109The leaven of CFU/L, reaches 3.5 at 37 DEG C of bottom fermentations to material liquid pH value, obtains fermentation liquid.
10. the preparation method of hepatoprotective plant oral liquid according to claim 7, it is characterized in that, in step (3), described leaven is mass ratio is Lactobacillus bulgaricus: lactobacillus casei: streptococcus thermophilus=2:1.5:1, or mass ratio is bacillus bifidus: Lactobacillus plantarum: the combination strain of lactobacillus rhamnosus=2:2:1.
11. the preparation method of hepatoprotective plant oral liquid according to claim 7, it is characterised in that in step (4), it is filtered described fermentation lixiviating solution processing, is placed at 6 DEG C by the fermentation liquid being filtrated to get ripening at low temperature 10 days, obtains ripening liquid.
12. the preparation method of the hepatoprotective plant oral liquid as in any of the one of claim 16, it is characterised in that it comprises the steps:
(1) Feedstock treating: clean and avoid Radix Puerariae to soak in water the plant raw material in above-mentioned raw materials with water, after draining, Radix Puerariae and the other plant class raw material except Radix Puerariae are separately used refiner homogenate, all add water homogenate than 1:4 by material water quality during homogenate, respectively obtain Radix Puerariae homogenate and the mixed plant homogenate except Radix Puerariae；
(2) enzymolysis: be separately added in described Radix Puerariae homogenate and respectively account for the amylase of its quality 0.1 ~ 1%, cellulase, it is separately added in the described mixed plant homogenate except Radix Puerariae and respectively accounts for the pectase of its quality 0.1 ~ 1%, cellulase, enzymolysis 4 ~ 16h at 37 ~ 45 DEG C, obtains Radix Puerariae primary enzymolysis liquid and the mixed plant primary enzymolysis liquid except Radix Puerariae；In above two primary enzymolysis liquid, add the acid protease accounting for its quality 0.1 ~ 1%, enzymolysis 4 ~ 10h at 37 ~ 45 DEG C respectively, obtain Radix Puerariae secondary enzymolysis liquid and the mixed plant secondary enzymolysis liquid except Radix Puerariae；
(3) one time fermentation: add the glucose in above-mentioned raw materials in described Radix Puerariae secondary enzymolysis liquid, the brown sugar (Saccharum Sinensis Roxb.) in above-mentioned raw materials is added in described mixed plant secondary enzymolysis liquid except Radix Puerariae, adding water, stirring to sugar is completely dissolved, then is added to 0.1 × 10 respectively9~1×109The leaven of CFU/L, reaches 3.5 ~ 4 at 37 ~ 40 DEG C of bottom fermentations to material liquid pH value, obtains Radix Puerariae fermentation liquid and the mixed plant fermentation liquid except Radix Puerariae；
Described leaven is the combination strain being made up of two or three in Lactobacillus bulgaricus, lactobacillus casei, streptococcus thermophilus, bacillus bifidus, Lactobacillus plantarum, lactobacillus rhamnosus, bacillus acidophilus；
(4) ferment in second time: the Radix Puerariae fermentation liquid respectively step (3) obtained and the mixed plant fermentation liquid except Radix Puerariae are filtered processing, the Radix Puerariae fermentating liquid filtrate obtained and the mixed plant fermentating liquid filtrate except Radix Puerariae are respectively taken in right amount and mix according to a certain percentage, in mixed filtrate, adds 0.1 × 109~1×109The leaven of CFU/L, reaches 3 ~ 3.5 at 37 ~ 40 DEG C of bottom fermentations to material liquid pH value, obtains ferment in second time liquid；
Described leaven is the combination strain being made up of two or three in Lactobacillus bulgaricus, lactobacillus casei, streptococcus thermophilus, bacillus bifidus, Lactobacillus plantarum, lactobacillus rhamnosus, bacillus acidophilus；
(5) filter, ripening: be filtered described ferment in second time liquid processing, the filtrate obtained be placed at 4 DEG C ~ 10 DEG C ripening at low temperature 4 ~ 60 days, obtain ripening liquid；
(6) allotment: add other raw materials except plant raw material and sugar, stirring and dissolving in described ripening liquid, adds appropriate citric acid and glucose regulates sugar-acid ratio, moderate to mouthfeel；
(7) fill sterilizing: liquid deployed for step (6) is carried out fill, sealing at dust proof workshop with 0.45 μm of membrane filtration and by every bottle of 30ml specification, then at 80 ~ 120 DEG C sterilizing 20 ~ 30min, namely obtain hepatoprotective plant oral liquid product.
13. the preparation method of hepatoprotective plant oral liquid according to claim 12, it is characterized in that, in step (4), described leaven is mass ratio is bacillus bifidus: Lactobacillus plantarum: lactobacillus rhamnosus=2:2:1, or mass ratio is lactobacillus rhamnosus: the combination strain of bacillus acidophilus=2:1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610083663.3A CN105707765A (en) | 2016-02-06 | 2016-02-06 | Liver protection plant oral liquid and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610083663.3A CN105707765A (en) | 2016-02-06 | 2016-02-06 | Liver protection plant oral liquid and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105707765A true CN105707765A (en) | 2016-06-29 |
Family
ID=56156619
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610083663.3A Pending CN105707765A (en) | 2016-02-06 | 2016-02-06 | Liver protection plant oral liquid and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105707765A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106721699A (en) * | 2016-11-30 | 2017-05-31 | 江苏食品药品职业技术学院 | A kind of sobering-up beverage and preparation method thereof |
| CN107006747A (en) * | 2017-04-20 | 2017-08-04 | 南昌大学 | A kind of preparation method of the haw fermented beverage of Soboring-up liver-protecting |
| CN108391813A (en) * | 2018-04-02 | 2018-08-14 | 齐鲁工业大学 | A kind of relieving alcoholism and protecting liver ferment and preparation method thereof |
| CN108704096A (en) * | 2018-06-04 | 2018-10-26 | 厦门元之道生物科技有限公司 | A kind of herb fermenting agent and preparation method thereof with reinforcing spleen to promote digestion function |
| CN108719989A (en) * | 2018-06-05 | 2018-11-02 | 济南航晨生物科技有限公司 | A kind of amino acid pattern containing pueraria lobata relieves the effect of alcohol ferment |
| CN114522218A (en) * | 2022-03-14 | 2022-05-24 | 唐建 | Composition for quickly dispelling effects of alcohol, protecting liver and preparation method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1110151A (en) * | 1994-04-07 | 1995-10-18 | 任贵兴 | Antialcoholic liver-protecting preparation and its preparing method |
| CN1698879A (en) * | 2005-06-21 | 2005-11-23 | 蔺益民 | Product having sobering up and liver protecting functions and its preparation method and usage |
| CN102188521A (en) * | 2010-03-19 | 2011-09-21 | 袁志辉 | Composition for relieving and neutralizing effect of alcohol |
| CN102335264A (en) * | 2011-10-09 | 2012-02-01 | 通化师范学院 | Actinidia arguta and kudzuvine root oral liquid for relieving alcoholism, and preparation method thereof |
| CN104643190A (en) * | 2013-11-16 | 2015-05-27 | 周国萍 | Liver-protection health drink |
| CN104839837A (en) * | 2015-03-30 | 2015-08-19 | 孙村镇中药材种植技术协会 | Partially fermented radix puerariae and Chinese mulberry juice helpful for dispelling alcohol and protecting liver and a preparation method thereof |
-
2016
- 2016-02-06 CN CN201610083663.3A patent/CN105707765A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1110151A (en) * | 1994-04-07 | 1995-10-18 | 任贵兴 | Antialcoholic liver-protecting preparation and its preparing method |
| CN1698879A (en) * | 2005-06-21 | 2005-11-23 | 蔺益民 | Product having sobering up and liver protecting functions and its preparation method and usage |
| CN102188521A (en) * | 2010-03-19 | 2011-09-21 | 袁志辉 | Composition for relieving and neutralizing effect of alcohol |
| CN102335264A (en) * | 2011-10-09 | 2012-02-01 | 通化师范学院 | Actinidia arguta and kudzuvine root oral liquid for relieving alcoholism, and preparation method thereof |
| CN104643190A (en) * | 2013-11-16 | 2015-05-27 | 周国萍 | Liver-protection health drink |
| CN104839837A (en) * | 2015-03-30 | 2015-08-19 | 孙村镇中药材种植技术协会 | Partially fermented radix puerariae and Chinese mulberry juice helpful for dispelling alcohol and protecting liver and a preparation method thereof |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106721699A (en) * | 2016-11-30 | 2017-05-31 | 江苏食品药品职业技术学院 | A kind of sobering-up beverage and preparation method thereof |
| CN107006747A (en) * | 2017-04-20 | 2017-08-04 | 南昌大学 | A kind of preparation method of the haw fermented beverage of Soboring-up liver-protecting |
| CN107006747B (en) * | 2017-04-20 | 2020-04-24 | 南昌大学 | Preparation method of sobering-up and liver-protecting hawthorn fermented beverage |
| CN108391813A (en) * | 2018-04-02 | 2018-08-14 | 齐鲁工业大学 | A kind of relieving alcoholism and protecting liver ferment and preparation method thereof |
| CN108704096A (en) * | 2018-06-04 | 2018-10-26 | 厦门元之道生物科技有限公司 | A kind of herb fermenting agent and preparation method thereof with reinforcing spleen to promote digestion function |
| CN108704096B (en) * | 2018-06-04 | 2021-01-12 | 厦门元之道生物科技有限公司 | Preparation method of traditional Chinese medicine leavening agent with spleen invigorating and digestion promoting functions |
| CN108719989A (en) * | 2018-06-05 | 2018-11-02 | 济南航晨生物科技有限公司 | A kind of amino acid pattern containing pueraria lobata relieves the effect of alcohol ferment |
| CN114522218A (en) * | 2022-03-14 | 2022-05-24 | 唐建 | Composition for quickly dispelling effects of alcohol, protecting liver and preparation method thereof |
| CN114522218B (en) * | 2022-03-14 | 2022-11-18 | 唐建 | Composition for rapidly dispelling effects of alcohol, protecting liver and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105707765A (en) | Liver protection plant oral liquid and preparation method thereof | |
| TWI500391B (en) | Composition for reliving and preventing hangover and beverage comprising composition | |
| CN104107217B (en) | The composition that prevention or treatment are still drank after a night | |
| CN103520577A (en) | Medicine-and-food dual-purpose preparation for treating pneumoconiosis, clearing away lung-heat and nourishing heart and preparation method for medicine-and-food dual-purpose preparation | |
| CN102726580A (en) | Lucid ganoderma tea, lucid ganoderma teabag and processing technology thereof | |
| CN106954843A (en) | A kind of composite plant enzyme stoste, feature composite plant enzyme stoste and preparation method thereof and functional food | |
| KR101687982B1 (en) | Composition for improving sexual functionality having effects of increasing of the number of sperm and protection of environmental hormone and manufacturing method thereof | |
| CN110432414A (en) | A kind of Dendrobidium huoshanness combined beverage and preparation method thereof adjusting function of human body | |
| CN107904103A (en) | A kind of dendrobium candidum health liquor and its preparation method and application | |
| WO2021114586A1 (en) | Immune response-improving composition, preparation method and application thereof | |
| CN106343567A (en) | Noni oral liquid and preparing method thereof | |
| CN110604800A (en) | Alcohol-dispelling liver-protecting plant beverage for abstinence of alcohol and preparation method thereof | |
| KR101588378B1 (en) | Method for manufacturing hangover curing agent and hangover curing agent manufactured by the same | |
| KR101394358B1 (en) | Health composition relieving alcohol hangover | |
| CN103828988A (en) | Lucid ganoderma tea and preparation method thereof | |
| CN103960663A (en) | Health care food for enhancing immunologic function and preparation method of food | |
| KR20180000874A (en) | Nutritional food composition helping detoxification or elimiation of fine dust, heavy metals and antibiotics, and its manufacturing method | |
| CN105379885A (en) | Moderate stomach-nourishing type ancient-prescription herbal tea | |
| CN105613848A (en) | Sober-up liver-protection health-care tea and preparation method thereof | |
| CN111643563B (en) | Composition for harmonizing stomach, protecting liver and dispelling effects of alcohol and preparation method thereof | |
| CN107440114A (en) | A kind of composition with anti-PM2.5 particulate matters effect | |
| CN112450396A (en) | Clausena lansium compound composition and preparation method and application thereof | |
| CN108338098A (en) | A kind of cultural method of selenium-rich bullhead | |
| CN115607629B (en) | Traditional Chinese medicine composition with liver protecting and alcohol effect dispelling functions and preparation method thereof | |
| CN102631487B (en) | Traditional Chinese medicine composition capable of improving immunity and resisting fatigue |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160629 |