CN105699370B - Amniotic fluid detects director composition, its preparation and corresponding amniotic fluid detection protection pad - Google Patents
Amniotic fluid detects director composition, its preparation and corresponding amniotic fluid detection protection pad Download PDFInfo
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- CN105699370B CN105699370B CN201610036848.9A CN201610036848A CN105699370B CN 105699370 B CN105699370 B CN 105699370B CN 201610036848 A CN201610036848 A CN 201610036848A CN 105699370 B CN105699370 B CN 105699370B
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- amniotic fluid
- protection pad
- fluid detection
- director composition
- detection protection
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/775—Indicator and selective membrane
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Abstract
The invention discloses amniotic fluid to detect director composition, it is prepared and corresponding amniotic fluid detection protection pad, the amniotic fluid detection director composition includes with water, ethyl alcohol, acetone, N, one or both of N dimethylformamides are solvent, with pulullan polysaccharide, sodium alginate, ethyl cellulose, one or more in sodium carboxymethylcellulose are film forming agent, using dioctyl phthalate as plasticiser, with ethylene glycol, glycerine, ethylene glycol ethyl ether is wetting agent, with bromthymol blue sodium salt, xylene bromide phenol is blue, sodium alizarine sulphonate, bromocresol purple is indicator, amniotic fluid detection indicator is prepared at normal temperatures, then it is coated onto on medical sanitary pad, drying is prepared into amniotic fluid detection protection pad.The present invention is easy to use, is not easy to decolourize, it is easier to be received by patient, and can distinguish amniotic fluid and urine, entire technological operation is simple, it is easy to accomplish large-scale production.
Description
Technical field
The invention belongs to medical dressing field, it is related to a kind of amniotic fluid detection director composition, preparation method and corresponding
Amniotic fluid detection protection pad.
Background technology
Rupture of membranes (ROM) may at any time occur during the pregnancy period, be ruptured under normal circumstances in term birth, but such as
Fruit occurs surprisingly suspender for pregnant woman will being given to carry out various pains in antenatal too early rupture.Fetal membrane is ruptured before term birth, referred to as tire
Film is early broken or PROM；The incidence of premature rupture of fetal membranes PROM is about 10% after 37 pregnant weeks under normal conditions, the generation of ROM before 37 pregnant weeks
Rate is 2-3.5%, referred to as PPROM.PROM is to cause an antenatal and postpartum complication principal element, is to lead to premature labor and must
The main reason for neonatal complications of NICU treatments must be moved in.Due to medical worker have to extend pregnant week with it is in utero and pregnant
Woman infection risk and fetus lung development problem between seek balance, difficulty not only of high cost to the management of PROM and PPROM patients but also
Greatly.Thus, correct, timely diagnosis is very crucial.
At this stage for the diagnosis of PROM, only often it is not enough to confirm the state of fetal membrane with medical history and physical examination.At present
Detection method is broadly divided into：1. supersonic testing method；2.pH test paper detection methods；3. insulin-like growth factor binding protein detects
Method.But amniotic fluid leakage rate is smaller, and the amniotic fluid assessed may be can't see in vagina, amniotic fluid is also possible to viscous with urine, uterine neck
The pollutions such as liquid, vaginal fluid, blood or meconium, to cause the error of detection.Patent CN201220572286.7 is disclosed
PH test paper is positioned in protection pad by a kind of amniotic fluid detection protection pad, and the pH by detecting vagina omission timber, which changes to detect amniotic fluid, is
No leakage, but when containing urine in vagina omission timber, pH test paper may still change colour, therefore amniotic fluid and urine cannot be distinguished,
To easily cause erroneous judgement, and pH test paper encounters easy decoloration when urine or amniotic fluid, to pollute contacted skin and clothing
Object.Patent CN201520302623.4 is disclosed a kind of amniotic fluid detection health-care protection pad and is passed through using anthocyanidin as indicator
The pH of detection vagina omission timber changes to judge whether amniotic fluid leaks, but amniotic fluid and urine still cannot be distinguished in the method, and
And color change unobvious, it be easy to cause erroneous judgement.Patent CN03824113.7 discloses amniotic fluid in a kind of detection vaginal fluid
Device and method, antibody is detected by PAMG-1 and detects a small amount of amniotic fluid in vagina, detection method sensitivity is higher, still
Detection swab, which generally requires, gos deep into vagina 5-7cm, is easy to carry out sense of discomfort to suspender for pregnant woman, and there are certain risks.
As it can be seen that in this field there are still for it is easy to use, be not easy to decolourize, be easy to be received, compared with the Supreme People's Procuratorate by patient
It surveys sensitivity, amniotic fluid can be distinguished and the demand of the amniotic fluid detection product of urine.
Invention content
Present invention aims at provide it is a kind of it is easy to use, be not easy to decolourize, significantly and amniotic fluid and urine can be easily distinguished
The amniotic fluid of liquid detects product, specifically includes amniotic fluid detection director composition and is prepared by amniotic fluid detection director composition
Amniotic fluid detection protection pad.
To achieve the above object, the present invention uses following technical scheme：Amniotic fluid detects director composition comprising：
80%-99%（w/w）The solvent selected from water, ethyl alcohol, acetone, one or both of N,N-dimethylformamide；0.1%-5%
（w/w）The film forming selected from pulullan polysaccharide, ethyl cellulose, sodium alginate, one or both of sodium carboxymethylcellulose
Agent；0.05%-3%（w/w）The plasticiser selected from dioctyl phthalate；0.5%-10%（w/w）Selected from ethylene glycol, the third three
The wetting agent of one or more of alcohol, ethylene glycol ethyl ether；And 0.01%-1%（w/w）Selected from bromthymol blue sodium salt,
The indicator of two or more in xylene bromide phenol indigo plant, sodium alizarine sulphonate, bromocresol purple.
The invention further relates to the preparation methods that above-mentioned amniotic fluid detects director composition comprising by the amniotic fluid indicator group
The each component for closing object is uniformly dissolved to obtain amniotic fluid detection director composition at normal temperatures.
The invention further relates to the amniotic fluid detection protection pads obtained by amniotic fluid detection director composition, pass through such as lower section
Method obtains：
(1) preparation of amniotic fluid detection director composition：
The each component that amniotic fluid in claim 1 is detected to director composition is uniformly dissolved to obtain amniotic fluid inspection at normal temperatures
Survey director composition；
(2) preparation of amniotic fluid detection protection pad：
The amniotic fluid being prepared in step (1) detection director composition is uniformly applied on medical sanitary pad, so
After dry 3-30h, obtain amniotic fluid detection protection pad.
Protection pad drying temperature is in the present invention：20-50℃.
Applying amount of the amniotic fluid detection indicator on Medical protective pad is in the present invention：0.01-0.5ml/cm2。
The advantage of the invention is that：
The amniotic fluid detection protection pad of preparation is easy to use, is not easy to decolourize, it is easier to be received by patient, and can distinguish amniotic fluid
And urine, entire technological operation are simple, it is easy to accomplish large-scale production.
Specific implementation mode：
Embodiment 1
1.0g pulullan polysaccharides are weighed, are dissolved in 67g water, after stirring and dissolving is uniform, sequentially add 0.73g neighbours
Dioctyl phthalate, 0.93g ethylene glycol are uniformly mixing to obtain solution A under room temperature (25 DEG C).
16mg bromthymol blues sodium salt and 10mg sodium alizarine sulphonates are weighed, is placed in 1.5g water and is uniformly dissolved to obtain solution B,
Then solution A and B solution are mixed evenly to obtain amniotic fluid detection indicator under room temperature (25 DEG C).Take above-mentioned indicator molten
Liquid is according to 0.1ml/cm2Dosage be equably applied on medical sanitary pad, be then made after 50 DEG C of dry 10h amniotic fluid detection
Protection pad.
Embodiment 2
1.0g ethyl celluloses are weighed, are dissolved in 40g ethyl alcohol, after stirring and dissolving is uniform, sequentially add 0.52g
Dioctyl phthalate, 1.02g glycerine, are uniformly mixing to obtain solution A.
12mg xylene bromide phenol indigo plants and 15mg bromocresol purples are weighed, is placed in 2g water and is uniformly dissolved to obtain solution B, then will
Solution A is mixed evenly to obtain amniotic fluid detection indicator with B solution.Take above-mentioned indicator solution according to 0.07ml/cm2Use
Amount is equably applied on medical sanitary pad, and amniotic fluid detection protection pad then is made after 45 DEG C of dry 15h.
Embodiment 3
1.0g sodium alginates are weighed, are dissolved in 70g water, after stirring and dissolving is uniform, sequentially add 0.63g neighbour's benzene
Diformazan dioctyl phthalate, 0.75g ethylene glycol ethyl ethers, are uniformly mixing to obtain solution A.
7mg bromthymol blues sodium salt, 5mg xylene bromides phenol indigo plant, 10mg bromocresol purples are weighed, is placed in 2.5g water and dissolves
It is even to obtain solution B, then solution A and B solution are mixed evenly to obtain amniotic fluid detection indicator.Take above-mentioned indicator solution
According to 0.1ml/cm2Dosage be equably applied on medical sanitary pad, be then made after 35 DEG C of dry 20h amniotic fluid detection shield
Pad.
Embodiment 4
1.0g sodium carboxymethylcelluloses are weighed, are dissolved in 80g water, after stirring and dissolving is uniform, are sequentially added
0.94g dioctyl phthalates, 1.21g ethylene glycol, are uniformly mixing to obtain solution A.
It is blue to weigh blue 15mg xylene bromides phenol, 5mg bromocresol purples and 13mg xylene bromides phenol, is placed in 1.5g water and dissolves
It is even to obtain solution B, then solution A and B solution are mixed evenly to obtain amniotic fluid detection indicator.Take above-mentioned indicator solution
According to 0.2ml/cm2Dosage be equably applied on medical sanitary pad, be then made after 30 DEG C of dry 30h amniotic fluid detection shield
Pad.
Comparative example 1
16mg bromthymol blues sodium salt and 10mg sodium alizarine sulphonates are weighed, is placed in 1.5g water and is uniformly dissolved to obtain solution,
Take above-mentioned indicator solution according to 0.1ml/cm2Dosage be equably applied on medical sanitary pad, then 50 DEG C of dry 10h
After amniotic fluid detection protection pad is made.
Comparative example 2
Commercially available pH test paper（Range is 1-14）.
Comparative example 3
1 detection kit of commercially available insulin-like growth factor binding protein-.
Product decoloration contrast test
The product of Example 1-4 and comparative example 1-3 are cut into strip of the same area（1cm×4cm）,
Then it is immersed in respectively and is filled in the buffer solution that 100ml pH are 5.0, pull sample after separated in time out
Then product stir the water in beaker with glass bar, the color change of water in the cup is burnt in observation after making color diffusion uniformly, and records number
According to：
Compared with contrast groups 1-2, the color fastness of sample prepared by embodiment 1-4 is higher, only has after 30 minutes slight
Decoloration, and with the growth of time, bleaching level does not increase, and product prepared by contrast groups 1-2 goes out rapidly into the water
It now decolourizes phenomenon, and decoloration gradually increases in the short time, color throw is than more serious, and product is not due to having color in comparative example 3
Plain ingredient, therefore without decoloration phenomenon.
Product comfort level compares
The sample of Example 1-4 and comparative example 1-3 obtain the comfortable degrees of data of product according to the analysis of application method：
Compared with contrast groups 2-3, the amniotic fluid detection protection pad and the conventional protection pad that are prepared with comparative example 1 using embodiment 1-4 are made
Consistent with method, comfort level is higher, but amniotic fluid detection protection pad prepared by comparative example 1 has a slight decoloration, and comparative example 2
In commercially available pH test paper operability it is poor, it is not only unhygienic but also inconvenient.3 product of comparative example is invasive relatively strong, is easy to make to pregnant woman
At the sense of urgency, comfort is poor.
Amniotic fluid and urine detection experiment
It is first used wetted with urine by the product of Example 1-4 and comparative example 1-3, observes color change situation, then will
It is respectively placed in dry place and dries 30min, then observes color change situation, and records data.
Test result：
The product of Example 1-4 and comparative example 1-2 first soak it with amniotic fluid, observe color change situation, then will
It is respectively placed in dry place and dries 30min, then observes color change situation, and records data.
Test result：
It compared with contrast groups 2, meets amniotic fluid using amniotic fluid detection protection pad prepared by embodiment 1-4 and becomes blue-green, and meet urine
Non-discolouring or change blue-green, after meeting urine change blue-green, bluish-green decoloration becomes faint yellow after placing 30 minutes, and meets amniotic fluid
After becoming blue-green, become dark blue-green after placing 30 minutes, so amniotic fluid detection protection pad prepared by embodiment 1-4 completely can be with area
Amniotic fluid and urine are separated, and the amniotic fluid in comparative example 1 monitors protection pad and meets urine as commercially available pH Test papers in comparative example 2
Both all become light blue with amniotic fluid, so cannot be distinguished.3 product of comparative example chance urine is non-discolouring, meets amniotic fluid and becomes red, can be with area
Divide amniotic fluid and urine, but is detected since swab is goed deep into vagina 5-7cm by its needs, so be easy to carry out sense of discomfort to suspender for pregnant woman,
Inconvenient to use, comfort is poor.
In conclusion the amniotic fluid detection protection pad that is produced of method of the present invention has easy to use, it is not easy to decolourize, it is easier to
Received by patient, and amniotic fluid and urine can be distinguished, the product being significantly better than in comparative example 1-3.
The preferred embodiment of the present invention has been described above in detail, still, during present invention is not limited to the embodiments described above
Detail can carry out a variety of simple variants to technical scheme of the present invention within the scope of the technical concept of the present invention, this
A little simple variants all belong to the scope of protection of the present invention.
It is further to note that specific technical features described in the above specific embodiments, in not lance
In the case of shield, can be combined by any suitable means, in order to avoid unnecessary repetition, the present invention to it is various can
The combination of energy no longer separately illustrates.
In addition, various embodiments of the present invention can be combined randomly, as long as it is without prejudice to originally
The thought of invention, it should also be regarded as the disclosure of the present invention..
Claims (5)
1. a kind of amniotic fluid detects director composition comprising：80%-99%'s (w/w) is selected from water, ethyl alcohol, acetone, N, N- bis-
The solvent of one or both of methylformamide；0.1%-5%'s (w/w) is selected from pulullan polysaccharide, ethyl cellulose, alginic acid
The film forming agent of one or both of sodium, sodium carboxymethylcellulose；0.05%-3% (w/w) selected from dioctyl phthalate
Plasticiser；The wetting agent selected from one or more of ethylene glycol, glycerine, ethylene glycol ethyl ether of 0.5%-10% (w/w)；
With the indicator chosen from the followings of 0.01%-1% (w/w)：The combination of bromthymol blue sodium salt and sodium alizarine sulphonate, xylene bromide phenol
Blue and bromocresol purple combination or the combination of bromthymol blue sodium salt, xylene bromide phenol indigo plant and bromocresol purple.
2. the preparation method of the amniotic fluid director composition of claim 1 comprising by each group of the amniotic fluid director composition
Divide and is uniformly dissolved to obtain amniotic fluid detection director composition at normal temperatures.
3. a kind of amniotic fluid detection protection pad, obtains by the following method：
(1) preparation of amniotic fluid detection director composition：
The each component that amniotic fluid in claim 1 is detected to director composition is uniformly dissolved to obtain amniotic fluid and detect to refer at normal temperatures
Show agent composition；
(2) preparation of amniotic fluid detection protection pad：
The amniotic fluid being prepared in step (1) detection director composition is uniformly applied on medical sanitary pad, is then dried
Dry 3-30h, obtains amniotic fluid detection protection pad.
4. amniotic fluid detection protection pad according to claim 3, it is characterised in that drying temperature is in step (2)：20-50℃.
5. amniotic fluid detection protection pad according to claim 3, it is characterised in that amniotic fluid detection director composition is being cured in step (2)
It is with the applying amount on protection pad：0.01-0.5ml/cm2。
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610036848.9A CN105699370B (en) | 2016-01-20 | 2016-01-20 | Amniotic fluid detects director composition, its preparation and corresponding amniotic fluid detection protection pad |
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| CN201610036848.9A CN105699370B (en) | 2016-01-20 | 2016-01-20 | Amniotic fluid detects director composition, its preparation and corresponding amniotic fluid detection protection pad |
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| CN105699370A CN105699370A (en) | 2016-06-22 |
| CN105699370B true CN105699370B (en) | 2018-09-18 |
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| CN107576653B (en) * | 2017-06-24 | 2023-03-17 | 中瀚利加（北京）科技有限公司 | Composition for identifying amniotic fluid of pregnant woman |
| CN109900692B (en) * | 2019-04-04 | 2021-11-26 | 青岛大学 | Preparation method of gel for green and efficient detection of mercury ions and application of gel |
| CN109900691B (en) * | 2019-04-04 | 2021-11-19 | 青岛大学 | Test paper for rapidly detecting mercury ions, preparation method and application thereof |
| CN109900690B (en) * | 2019-04-04 | 2021-12-14 | 青岛大学 | Preparation method and application of sol for green rapid detection of sulfur ions |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101309929A (en) * | 2005-09-15 | 2008-11-19 | 创源生物科技（武汉）有限公司 | A marker for prolonged rupture of membranes |
| CN102297963A (en) * | 2011-05-30 | 2011-12-28 | 吉权 | Premature rupture of membrane colloidal gold test paper reaction buffer, and preparation method thereof |
| CN103999782A (en) * | 2014-06-05 | 2014-08-27 | 江苏中恒宠物用品股份有限公司 | Production technology for pet urinal pad having disease detection function |
| CN204636718U (en) * | 2015-05-12 | 2015-09-16 | 辛琰琰 | Amniotic fluid detects health-care protection pad |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20010025140A1 (en) * | 1998-07-22 | 2001-09-27 | Torok Brian A. | System for identifying premature rupture of membrane during pregnancy |
| US6627394B2 (en) * | 2001-07-19 | 2003-09-30 | Common Sense Ltd. | Diagnostic pad |
| SI1535068T1 (en) * | 2002-08-13 | 2010-07-30 | N Dia Inc | Devices and methods for detecting amniotic fluid in vaginal secretions |
| US7935538B2 (en) * | 2006-12-15 | 2011-05-03 | Kimberly-Clark Worldwide, Inc. | Indicator immobilization on assay devices |
-
2016
- 2016-01-20 CN CN201610036848.9A patent/CN105699370B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101309929A (en) * | 2005-09-15 | 2008-11-19 | 创源生物科技（武汉）有限公司 | A marker for prolonged rupture of membranes |
| CN102297963A (en) * | 2011-05-30 | 2011-12-28 | 吉权 | Premature rupture of membrane colloidal gold test paper reaction buffer, and preparation method thereof |
| CN103999782A (en) * | 2014-06-05 | 2014-08-27 | 江苏中恒宠物用品股份有限公司 | Production technology for pet urinal pad having disease detection function |
| CN204636718U (en) * | 2015-05-12 | 2015-09-16 | 辛琰琰 | Amniotic fluid detects health-care protection pad |
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