CN105686006B - One seed oyster composite function food and preparation method thereof - Google Patents
One seed oyster composite function food and preparation method thereof Download PDFInfo
- Publication number
- CN105686006B CN105686006B CN201610072106.1A CN201610072106A CN105686006B CN 105686006 B CN105686006 B CN 105686006B CN 201610072106 A CN201610072106 A CN 201610072106A CN 105686006 B CN105686006 B CN 105686006B
- Authority
- CN
- China
- Prior art keywords
- oyster
- lys
- parts
- composite function
- gly
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000237502 Ostreidae Species 0.000 title claims abstract description 69
- 235000020636 oyster Nutrition 0.000 title claims abstract description 69
- 235000013305 food Nutrition 0.000 title claims abstract description 34
- 239000002131 composite material Substances 0.000 title claims abstract description 31
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- MVORZMQFXBLMHM-QWRGUYRKSA-N Gly-His-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)CN)CC1=CN=CN1 MVORZMQFXBLMHM-QWRGUYRKSA-N 0.000 claims abstract description 26
- 108010038983 glycyl-histidyl-lysine Proteins 0.000 claims abstract description 25
- 241000208340 Araliaceae Species 0.000 claims abstract description 20
- 235000008434 ginseng Nutrition 0.000 claims abstract description 20
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims abstract description 19
- 235000003140 Panax quinquefolius Nutrition 0.000 claims abstract description 19
- 241001165494 Rhodiola Species 0.000 claims abstract description 16
- 239000000203 mixture Substances 0.000 claims abstract description 14
- 239000002994 raw material Substances 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 239000000284 extract Substances 0.000 claims description 11
- 239000000843 powder Substances 0.000 claims description 11
- 239000002775 capsule Substances 0.000 claims description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 9
- 239000006228 supernatant Substances 0.000 claims description 9
- 239000012528 membrane Substances 0.000 claims description 7
- 239000000047 product Substances 0.000 claims description 7
- 235000019441 ethanol Nutrition 0.000 claims description 6
- 239000000796 flavoring agent Substances 0.000 claims description 6
- 235000019634 flavors Nutrition 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 6
- 238000010992 reflux Methods 0.000 claims description 6
- 150000003384 small molecules Chemical class 0.000 claims description 6
- 238000000227 grinding Methods 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 5
- 238000005119 centrifugation Methods 0.000 claims description 4
- 238000001728 nano-filtration Methods 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 4
- 108091005804 Peptidases Proteins 0.000 claims description 3
- 239000004365 Protease Substances 0.000 claims description 3
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 3
- 238000009835 boiling Methods 0.000 claims description 3
- 239000000919 ceramic Substances 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 239000006071 cream Substances 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims description 3
- 238000004108 freeze drying Methods 0.000 claims description 3
- 238000005469 granulation Methods 0.000 claims description 3
- 230000003179 granulation Effects 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 230000001376 precipitating effect Effects 0.000 claims description 3
- 238000004064 recycling Methods 0.000 claims description 3
- 238000007873 sieving Methods 0.000 claims description 3
- 239000011122 softwood Substances 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 238000005507 spraying Methods 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 238000000108 ultra-filtration Methods 0.000 claims description 3
- 239000002671 adjuvant Substances 0.000 claims description 2
- 238000006243 chemical reaction Methods 0.000 claims description 2
- 238000005138 cryopreservation Methods 0.000 claims description 2
- 239000002904 solvent Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 27
- 239000003814 drug Substances 0.000 abstract description 8
- 230000002195 synergetic effect Effects 0.000 abstract description 2
- 238000009472 formulation Methods 0.000 abstract 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 38
- 230000006870 function Effects 0.000 description 29
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 24
- 229920002527 Glycogen Polymers 0.000 description 17
- 229940096919 glycogen Drugs 0.000 description 17
- 230000009182 swimming Effects 0.000 description 17
- 239000004310 lactic acid Substances 0.000 description 12
- 235000014655 lactic acid Nutrition 0.000 description 12
- 210000002966 serum Anatomy 0.000 description 11
- PNNCWTXUWKENPE-UHFFFAOYSA-N [N].NC(N)=O Chemical compound [N].NC(N)=O PNNCWTXUWKENPE-UHFFFAOYSA-N 0.000 description 10
- 210000004369 blood Anatomy 0.000 description 10
- 239000008280 blood Substances 0.000 description 10
- 235000013376 functional food Nutrition 0.000 description 10
- 230000002929 anti-fatigue Effects 0.000 description 8
- 230000002440 hepatic effect Effects 0.000 description 8
- 230000033001 locomotion Effects 0.000 description 8
- 239000000463 material Substances 0.000 description 8
- 238000011049 filling Methods 0.000 description 7
- 210000003205 muscle Anatomy 0.000 description 7
- 210000003734 kidney Anatomy 0.000 description 6
- 210000004185 liver Anatomy 0.000 description 6
- 210000000988 bone and bone Anatomy 0.000 description 5
- 230000007423 decrease Effects 0.000 description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- 230000007812 deficiency Effects 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 150000004676 glycans Chemical class 0.000 description 4
- 229920001282 polysaccharide Polymers 0.000 description 4
- 239000005017 polysaccharide Substances 0.000 description 4
- 101710088194 Dehydrogenase Proteins 0.000 description 3
- 244000241838 Lycium barbarum Species 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 241000699670 Mus sp. Species 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 230000004099 anaerobic respiration Effects 0.000 description 3
- 208000006673 asthma Diseases 0.000 description 3
- 235000009508 confectionery Nutrition 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 229930003944 flavone Natural products 0.000 description 3
- 235000011949 flavones Nutrition 0.000 description 3
- 238000003304 gavage Methods 0.000 description 3
- 201000001881 impotence Diseases 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 229940100688 oral solution Drugs 0.000 description 3
- 230000000144 pharmacologic effect Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 241000208688 Eucommia Species 0.000 description 2
- 235000015459 Lycium barbarum Nutrition 0.000 description 2
- LCTONWCANYUPML-UHFFFAOYSA-N Pyruvic acid Chemical compound CC(=O)C(O)=O LCTONWCANYUPML-UHFFFAOYSA-N 0.000 description 2
- 230000001093 anti-cancer Effects 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000017531 blood circulation Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 150000002213 flavones Chemical class 0.000 description 2
- 235000013402 health food Nutrition 0.000 description 2
- 230000001965 increasing effect Effects 0.000 description 2
- 210000003127 knee Anatomy 0.000 description 2
- 210000004072 lung Anatomy 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 229940096978 oral tablet Drugs 0.000 description 2
- 230000035479 physiological effects, processes and functions Effects 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 238000005728 strengthening Methods 0.000 description 2
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- LTTQKYMNTNISSZ-MWTRTKDXSA-N (2S)-(-)-kurarinone Chemical compound C1([C@H]2OC=3C(C[C@@H](CC=C(C)C)C(C)=C)=C(O)C=C(C=3C(=O)C2)OC)=CC=C(O)C=C1O LTTQKYMNTNISSZ-MWTRTKDXSA-N 0.000 description 1
- YLRXAIKMLINXQY-ZDUSSCGKSA-O (S)-magnoflorine Chemical compound C1=C(OC)C(O)=C2C3=C(O)C(OC)=CC=C3C[C@@H]3[N+](C)(C)CCC1=C23 YLRXAIKMLINXQY-ZDUSSCGKSA-O 0.000 description 1
- 206010002383 Angina Pectoris Diseases 0.000 description 1
- 208000006820 Arthralgia Diseases 0.000 description 1
- 241000150786 Athletes Species 0.000 description 1
- 102100026189 Beta-galactosidase Human genes 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 206010008190 Cerebrovascular accident Diseases 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 241000220284 Crassulaceae Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000893536 Epimedium Species 0.000 description 1
- 241001362421 Epimedium brevicornu Species 0.000 description 1
- 206010019280 Heart failures Diseases 0.000 description 1
- 206010019468 Hemiplegia Diseases 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- 108010059881 Lactase Proteins 0.000 description 1
- KYEAXNAYHSCLMT-CVVGWEDFSA-N Magnoflorine Natural products C[C@H]1OC=C2[C@@H]3[C@@H]1CN4CCc5c([nH]c6ccccc56)[C@@H]4[C@@H]3OC2=O KYEAXNAYHSCLMT-CVVGWEDFSA-N 0.000 description 1
- 241000218922 Magnoliophyta Species 0.000 description 1
- 108010070551 Meat Proteins Proteins 0.000 description 1
- 206010062575 Muscle contracture Diseases 0.000 description 1
- DRBBFCLWYRJSJZ-UHFFFAOYSA-N N-phosphocreatine Chemical compound OC(=O)CN(C)C(=N)NP(O)(O)=O DRBBFCLWYRJSJZ-UHFFFAOYSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 206010067482 No adverse event Diseases 0.000 description 1
- 208000002193 Pain Diseases 0.000 description 1
- 206010033557 Palpitations Diseases 0.000 description 1
- 235000002791 Panax Nutrition 0.000 description 1
- 241000208343 Panax Species 0.000 description 1
- 240000004371 Panax ginseng Species 0.000 description 1
- 235000002789 Panax ginseng Nutrition 0.000 description 1
- 241001529246 Platymiscium Species 0.000 description 1
- 206010035664 Pneumonia Diseases 0.000 description 1
- 230000006819 RNA synthesis Effects 0.000 description 1
- 208000025747 Rheumatic disease Diseases 0.000 description 1
- 208000006011 Stroke Diseases 0.000 description 1
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
- 208000009205 Tinnitus Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000003178 anti-diabetic effect Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000003005 anti-senility effect Effects 0.000 description 1
- 239000003472 antidiabetic agent Substances 0.000 description 1
- 239000006286 aqueous extract Substances 0.000 description 1
- 238000003149 assay kit Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 108010005774 beta-Galactosidase Proteins 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000005252 bulbus oculi Anatomy 0.000 description 1
- 206010007625 cardiogenic shock Diseases 0.000 description 1
- 210000000748 cardiovascular system Anatomy 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000009194 climbing Effects 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 208000006111 contracture Diseases 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000006837 decompression Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000005059 dormancy Effects 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 235000018905 epimedium Nutrition 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 210000003414 extremity Anatomy 0.000 description 1
- 230000004438 eyesight Effects 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 150000002212 flavone derivatives Chemical class 0.000 description 1
- 230000034659 glycolysis Effects 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 210000003128 head Anatomy 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 235000008216 herbs Nutrition 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000001660 hyperkinetic effect Effects 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 230000007954 hypoxia Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 231100000567 intoxicating Toxicity 0.000 description 1
- 230000002673 intoxicating effect Effects 0.000 description 1
- XQVFLLMCNGKXSM-UHFFFAOYSA-N kurarinone Natural products COc1cc(O)c(C(CC=C(C)C)C(=C)C)c2OC(CC(=O)c12)c3ccc(O)cc3O XQVFLLMCNGKXSM-UHFFFAOYSA-N 0.000 description 1
- 229940116108 lactase Drugs 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000003212 lipotrophic effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 210000003470 mitochondria Anatomy 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000004118 muscle contraction Effects 0.000 description 1
- KDADHLPROOOPIC-UHFFFAOYSA-N neokurarinol Natural products COc1cc(O)ccc1C1CC(=O)c2c(OC)cc(O)c(CC(CCC(C)(C)O)C(C)=C)c2O1 KDADHLPROOOPIC-UHFFFAOYSA-N 0.000 description 1
- 231100000862 numbness Toxicity 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 238000001543 one-way ANOVA Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 229940023488 pill Drugs 0.000 description 1
- 239000006187 pill Substances 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 229940107700 pyruvic acid Drugs 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000000284 resting effect Effects 0.000 description 1
- 230000000552 rheumatic effect Effects 0.000 description 1
- 241001233863 rosids Species 0.000 description 1
- 210000001908 sarcoplasmic reticulum Anatomy 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 210000002027 skeletal muscle Anatomy 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 229960003080 taurine Drugs 0.000 description 1
- 230000035922 thirst Effects 0.000 description 1
- 231100000886 tinnitus Toxicity 0.000 description 1
- 230000004102 tricarboxylic acid cycle Effects 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
- 235000020985 whole grains Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/01—Hydrolysed proteins; Derivatives thereof
- A61K38/012—Hydrolysed proteins; Derivatives thereof from animals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/618—Molluscs, e.g. fresh-water molluscs, oysters, clams, squids, octopus, cuttlefish, snails or slugs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/29—Berberidaceae (Barberry family), e.g. barberry, cohosh or mayapple
- A61K36/296—Epimedium
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/41—Crassulaceae (Stonecrop family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/46—Eucommiaceae (Eucommia family), e.g. hardy rubber tree
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
- A61K36/815—Lycium (desert-thorn)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
- A61K9/1605—Excipients; Inactive ingredients
- A61K9/1617—Organic compounds, e.g. phospholipids, fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/4841—Filling excipients; Inactive ingredients
- A61K9/4858—Organic compounds
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Marine Sciences & Fisheries (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a seed oyster composite function foods, including following raw material to be made: 5~15 parts of oyster Gly-His-Lys, 1~3 part of ginseng, 2~6 parts of Herba Epimedii, 2~6 parts of root of kirilow rhodiola, 2~5 parts of fructus lycii, 3~6 parts of Cortex Eucommiae.The invention also discloses the preparation methods of the oyster composite function food.Inventive formulation makes various raw materials play good synergistic effect, can preferably play the curative effect of composition, and each Chinese medicine is made to play its unique effect, the significant effect in terms of alleviating physical fatigue.
Description
Technical field
The present invention relates to technical field of aquatic product processing, and in particular to a seed oyster composite function food and its preparation side
Method.
Background technique
In the health food of China's approval, fatigue resistant health food quantity comes third, it is sufficient to embody to antifatigue
Actual demand.Initially, it is believed that fatigue is that a series of intoxicating phenomenons caused by chemical change, performance occur because of body cell
For the even entire body ability to work decline of tissue, organ.With the exploration deeply and to tired mechanism of research, final science
Fatigue is not defined as to be continued by the caused organism physiology process of body movement itself its function in specified level or not by family
The phenomenon that capable of maintaining scheduled exercise intensity and locomitivity is caused to decline.
Oyster is Fresh & Tender in Texture, delicious flavour, there is nutritive value abundant, is known as " marine milk ".Modern scientific research table
Bright oyster meat protein, glycogen, taurine and microelement rich in have antifatigue, anti-oxidant, hypoglycemic and anti-swollen
The effects of tumor, acts not only as food, also has great medical value.Oyster be also China first be both food and
One of food materials of drug.
It extracts to obtain oyster polypeptide by complex enzyme hydrolysis from natural marine product oyster, further studies oyster active peptides
Physiological action, exploitation oyster health care product be of great significance.
Summary of the invention
The purpose of the present invention is to provide seed oyster composite function foods and preparation method thereof, and the functional food is with oyster
Gly-His-Lys are primary raw material, reasonably combined in conjunction with 5 taste extracts, have good antifatigue effect.
To achieve the above object, the invention adopts the following technical scheme:
One seed oyster composite function food, including following raw material are made: 5~15 parts of oyster Gly-His-Lys, ginseng 1~3
Part, 2~6 parts of Herba Epimedii, 2~6 parts of root of kirilow rhodiola, 2~5 parts of fructus lycii, 3~6 parts of Cortex Eucommiae.
Ginseng: also known as circle ginseng, kurarinone, wooden club, people's rank, terrible lid, god grass, soil essence, Goblin, belonging to Angiospermae has pipe to have embryo
Plant subphylum, Magnoliatae, Rosidae, Umbellales, Araliaceae, ginseng race, Panax.It is mild-natured for rare Chinese medicine, it is sweet in flavor, it is micro-
It is bitter.It breathes with cough for physically weak soft de-, cold weak pulse of limb, spleen eating less, the deficiency syndrome of the lung, injury thirst, Heat Diabetes, prolonged illness virtual winning, palpitation with fear mistake
Dormancy, impotence palace cold, heart failure, cardiogenic shock.Show that ginseng is tired to resisting for animal in the antifatigue experimental study of ginseng
Labor, resist oxygen lack, resisting stress and haematochrome increasing etc. all have apparent effect.Ginseng can enhance mouse hypoxia-bearing capability,
One of the reason of walking weight load extends, and antioxidation is raising fatigue resistance.The mechanism of Anti-Fatigue Effects of Panax ginseng can
Blood lipid can be increased with it and promotes protein, RNA synthesis related.Research shows that panaxoside and lipotropic component, can increase mouse
Autogenic movement, show antifatigue effect.
Herba Epimedii: Herba Epimedii is the help article of Chinese tradition, and history tree is on the books, nature and flavor acrid, sweet, warm；Return liver,
Kidney channel；With kidney-replenishing, the effect of strengthening bones and muscles, dispelling wind and dampness；For decline of kidney-YANG, impotence and seminal emission, muscles and bones impotence, rheumatic arthralgia,
Numbness contracture.Herba Epimedii has got tens of kinds of flavones ingredients, including Herba Epimedii using epimedium flavone as main component so far
Glycosides.Herba Epimedii also contains epimedium brevicornum polysaccharide.The Aqueous extracts of Folium Epimedii have apparent effect of scavenging radical.It is reported that in Herba Epimedii
Flavones ingredient magnoflorine has beneficial effect to biochemical indicators such as Hb, Glue, BLA, BUN before and after Athletess, and makes
Cardiovascular system is in hyperkinetic state, maintains high-intensity exercise ability conducive to sportsman's long period, and has resisting kinetic
The effect of fatigue.
Root of kirilow rhodiola: for Crassulaceae renascent herb or shrub plant, root of kirilow rhodiola is classified as in medicine in Shennong's Herbal
Product.It is sweet, it is bitter, it puts down, return lung, the heart channel of Hang-Shaoyin.With qi and activate blood circulation, the effect of promotes blood circulation and relievings asthma, it to be used for qi deficiency to blood stasis, chest impediment and cardialgia, apoplexy
Hemiplegia, burnout asthma.Modern pharmacological studies have shown that root of kirilow rhodiola can enhance exercise tolerance, anti-oxidant, anti-diabetic, anti-pneumonia and
The effects of asthma, anticancer.
Fructus lycii: fructus lycii is the dry mature fruit of Lycium barbarum L. of solanaceae Lycium barbarum L., wild
Have with cultivation, be distributed in the ground such as North China, northwest, also there is cultivation in other areas.Fructus lycii master contains polysaccharides, has extensive
Biological activity.Sterling polysaccharides can increase significantly mouse muscle glycogen, liver glycogen reserves amount；It improves before moving and swims
Serum lactic dehydrogenase (SLDH) total activity afterwards；Serum urea nitrogen increment after reduction mouse strenuous exercise accelerates blood urea nitrogen after moving
Regeneration rate.The result shows that sterling polysaccharides generates and adds in adaptability, resisting fatigue of the raising body to exercise load
Have the function of in terms of the elimination of fast fatigue fairly obvious.Show that there is lycium barbarum reduction blood lactase acid to contain by trial population
Amount effect, has anti-sports fatigue effect.
Cortex Eucommiae: being peculiar Dan Shu section, China, single platymiscium for famous Chinese medicine.It is managed according to chinese tradition integration of drinking and medicinal herbs
By Compendium of Material Medica is recorded, and Cortex Eucommiae has the health-care effect of " QI invigorating, hard muscles and bones " to human body.The Chinese patent drug made of Cortex Eucommiae is gone through
There is discussion for medical book is also more: being said in " longevity parent endowment new book ", Cortex Eucommiae ball can mend lower member, black beard and hair, strong foot knee, happy color, long term usage
Anti-aging.Says in " General Records of Holy Universal Relief ", Cortex Eucommiae drink can warm kidney sun, strengthening the bones and muscles, suitable for year the old and the weak insufficiency of the kidney yang, waist and knee crymodynia without
Power, curative effect are rather good.Says in " Renzhai Zhi Zhi Fang, Effective Recipes from Renzhai House opinion ", " Huanshao pill " (is formed) by medicinal material processings such as Cortex Eucommiaes, have nourishing liver and kidney, acute hearing ear,
The function of improving eyesight, body-building, by heart kidney deficiency, essence and blood is deficient, body virtual winning, the dark tinnitus person of mesh are common.Cortex Eucommiae has nourishing liver and kidney, strong
Muscles and bones, anti-senility control pain in the loins and other effects, the modern pharmacology proves that Cortex Eucommiae has immune, strong decompression, enhancing, anticancer, anti-grease
Various pharmacological actions such as matter peroxidating.It is confirmed through animal tissue's decomposition experiment, mouse mouse after taking Cortex Eucommiae decocting liquid
Showing the feature in skeletal muscle micro-structure is that connective tissue is flourishing, fibroblast activity is vigorous, collagenous fibres quantity increases
More, mitochondria and sarcoplasmic reticulum obviously enrich, this is adaptable with the supply of energy when contraction of muscle and the offer of Ca++, and
The structure basis of locomitivity is improved, to collagen synthesis and comprehensive locomitivity etc. that improves can be promoted to mention for Cortex Eucommiae
Direct morphological evidence is supplied.The swimming with a load attached to the body experimental study of mouse is also indicated that, eucommia wine extract can effectively extend weight bearing
Swimming time improves body glycogen deposit or reduces the consumption of glycogen when moving, enhances body to the adaptability of exercise load.
Therefore Cortex Eucommiae has antifatigue effect.
The invention also discloses the preparation methods of above-mentioned oyster composite function food, comprising the following steps:
S1. ginseng, Yin Yang Yu, root of kirilow rhodiola, fructus lycii, Cortex Eucommiae are taken according to the weight proportion of claim 1 and powdery is made.
S2. oyster Gly-His-Lys are prepared according to the following steps: (1) being beaten after taking fresh oyster cleaning to drain, the water of 2 times of volumes is added
Stirring；(2) trypsase 7500U/g, flavor protease 450U/g is added, adjusting pH is 8.0, and temperature is 40 DEG C~60 DEG C, instead
3h~4h is answered, until supernatant is extracted in supernatant and precipitating boundary clear, centrifugation；(3) supernatant obtained above is passed through into ceramics
Film removal of impurities, ultrafiltration membrance filter obtain small molecule oyster Gly-His-Lys of the molecular weight between 300~10000Da；(4) small molecule oyster peptide
Powder is concentrated by nanofiltration membrane, finally spray-dried to obtain oyster Gly-His-Lys.
S3. the ginseng of the obtained powdery of step S1, Yin Yang Yu, root of kirilow rhodiola, fructus lycii, Cortex Eucommiae and step S2 are obtained male
Oyster Gly-His-Lys are mixed to get composition powder according to the weight ratio.
S4. capsule or oral solution or tablet is made in obtained composition powder and customary adjuvant.
Wherein, in step S1 each raw material be made powdery process it is as follows: take dry Cortex Eucommiae, fructus lycii and root of kirilow rhodiola mixed
It closes, the water of 6 times of volumes is added, heating and refluxing extraction keeps 2h after reaching slight boiling condition, decocts and extract 2 times, before extracting for the first time
It impregnates；Merge 2 decoction liquors, filter, be concentrated with Rotary Evaporators, temperature is controlled at 50 DEG C~70 DEG C, is obtained just cream, is lyophilized into powder
Shape；Dry ginseng and Herba Epimedii mixing are taken, crushes, crosses 40 meshes, it is 6~10 times of matter of volume of mixture that volume is added after sieving
It measures the alcohol reflux that concentration is 80% to extract 2 times, recycling design, obtained paste is precipitated into freeze-drying powdering.
Wherein, step S2 small molecular oyster Gly-His-Lys are concentrated into solid content up to 10% by nanofiltration membrane, are finally spray-dried,
Controlling spraying inlet air temperature is 180 DEG C, and leaving air temp is 80 DEG C, obtains active oyster Gly-His-Lys, packs low temperature using initial vacuum
It saves.
Further, in step S4 capsule preparation process are as follows: the obtained composition powder of step S3 is crossed into 80 meshes, use
The ethyl alcohol that mass concentration is 85% is fabricated to softwood, the granulation of 16 meshes, and drying at a temperature of 50 DEG C~60 DEG C, 14 meshes are whole
Grain is packed into capsule shells to get capsule finished product.
After adopting the above technical scheme, the present invention has the advantage that the present invention wants raw material based on oyster Gly-His-Lys, in conjunction with
5 taste extracts are reasonably combined, and for the formula provided so that various raw materials play good synergistic effect, i.e., each Chinese medicine plays it
Unique effect, combining in terms of alleviating physical fatigue, effect is more significant, and prescription is more optimized, and active principle is extracted
More completely, the curative effect of composition is preferably played；And have no adverse reaction, pharmacological property is mild, and it is very high to take safety for a long time.Preparation
Equipment is simple, and cost is relatively low, can be realized industrialized production.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention
It is further elaborated.
The invention discloses a seed oyster composite function foods, want raw material based on oyster Gly-His-Lys, in conjunction with 5 taste extracts.
It is formulated as shown in table 1 below.
1. oyster composite function food list of ingredients of table
| Group | Oyster Gly-His-Lys (g) | Ginseng (g) | Herba Epimedii (g) | Root of kirilow rhodiola (g) | Fructus lycii (g) | Cortex Eucommiae (g) |
| One group | 5 | 1 | 2 | 2 | 2 | 3 |
| Two groups | 10 | 1 | 3 | 4 | 3 | 5 |
| Three groups | 15 | 3 | 6 | 6 | 5 | 6 |
The invention also discloses the preparation process of above-mentioned oyster composite function food, comprising the following steps:
S1. ginseng, Yin Yang Yu, root of kirilow rhodiola, fructus lycii, Cortex Eucommiae are taken according to the weight proportion of table 1 and powdery is made.Specifically such as
Under:
(1) dry Cortex Eucommiae, fructus lycii and root of kirilow rhodiola mixing are taken, the water of 6 times of volumes is added, heating and refluxing extraction reaches
2h is kept after slight boiling condition, decocts and extracts 2 times, is impregnated before extracting for the first time；Merge 2 decoction liquors, filters, use Rotary Evaporators
Concentration, temperature are controlled at 50 DEG C~70 DEG C, obtain just cream, powdering is lyophilized.
(2) dry ginseng and Herba Epimedii mixing are taken, crushes, crosses 40 meshes, it is volume of mixture 6 that volume is added after sieving
The alcohol reflux that~10 times of mass concentrations are 80% extracts 2 times, recycling design, and obtained paste is precipitated freeze-drying powdering.
S2. oyster Gly-His-Lys are prepared according to the following steps:
(1) it is beaten after taking fresh oyster cleaning to drain, the water stirring of 2 times of volumes is added.
(2) trypsase 7500U/g, flavor protease 450U/g is added, adjusting pH is 8.0, and temperature is 40 DEG C~60 DEG C,
3h~4h is reacted, until supernatant is extracted in supernatant and precipitating boundary clear, centrifugation.
(3) by supernatant obtained above by ceramic membrane clean, ultrafiltration membrance filter obtain molecular weight be 300~
Small molecule oyster Gly-His-Lys between 10000Da.
(4) small molecule oyster Gly-His-Lys are finally spray-dried, control spraying by being concentrated into solid content up to 10% to filter membrane
Inlet air temperature is 180 DEG C, and leaving air temp is 80 DEG C, obtains active oyster Gly-His-Lys, packs cryo-conservation using initial vacuum.
S3. the ginseng of the obtained powdery of step S1, Yin Yang Yu, root of kirilow rhodiola, fructus lycii, Cortex Eucommiae and step S2 are obtained male
Oyster Gly-His-Lys are mixed to get composition powder according to the weight proportion of table 1.
S4. the obtained composition powder of step S3 is crossed into 80 meshes, is fabricated to softwood with the ethyl alcohol that mass concentration is 85%,
The granulation of 16 meshes, and it is dry at a temperature of 50 DEG C~60 DEG C, and 14 mesh sieves are packed into capsule shells to get capsule finished product.Or with it is normal
It advises auxiliary material and oral solution or tablet is made.
Stomach-filling mouse experiment is carried out to the oyster composite function food for using preparation method of the invention to be prepared below,
Functional food of the present invention is inquired into the active function of mouse anti-reflecting fatigue by the measurement to mouse movement index and biochemical indicator.
1. animal packet and medication
SPF grades male ICR mouse 80, adaptable fed is randomly divided into 2 groups, every group 40 after a week, according to mouse weight
Only.That is blank control group, functional food group.Oral solution made from formula of the functional food group using two groups in table 1.Wherein blank
The daily stomach-filling 0.5ml distilled water of control group, the daily stomach-filling 1.0g/kg dosage oyster composite function food of functional food group.Simultaneously
It feeds mouse and maintains feed, free water is tested after 30d.
2. experimentation
(1) swimming time measures
Orally continuously give tested material 30d, after last stomach-filling 30min, by the mouse of 5% weight sheet lead of tail portion load as
Swimming trunk went swimming.The depth of water is no less than 30cm, 25 ± 1.0 DEG C of water temperature, sinks to the water surface with mouse head and continues 8s and be unable to emersion and be
Determine terminal, record mouse from swimming to the time of death, i.e. mouse swimming time.
(2) urea nitrogen content measures
Orally continuously give tested material 30d, after last stomach-filling 30min, the not swimming with a load attached to the body in the water that temperature is 30 DEG C
Eyeball is pulled out after 90min, rest 60min and takes blood, and 2000 turns of centrifugation 15min after waiting for blood to solidify take serum, with urea nitrogen testing cassete
Measurement.
(3) hepatic glycogen content measures
Tested material 30d is continuously orally given, cervical dislocation is put to death after last stomach-filling 30min, is taken liver, is floated through physiological saline
It washes, filter paper blots, and accurately weighs 100mg liver, and 30 μ L lye are added and boil rear flowing water cooling, is prepared into glycogen detection liquid, uses
The measurement of hepatic glycogen assay kit.
(4) Serum lactic acid content measures
Tested material 30d is continuously orally given, makes each group mouse in 30 DEG C of water temperature of water tank without negative after last stomach-filling 30min
Revisit swimming.Different times, the orbital venous plexus such as rest 60min take blood before swimming, after swim 90min, swimming, use reagent
Box measures mouse BLA content.
(5) data statistics and analysis
Data analysis carries out one-way analysis of variance using SPSS19.0 software and carries out LSD Multiple range test.
3. experimental result
(1) influence of the oyster composite function food to mouse weight
During test, mouse claims a weight every other week and records.Table 2 is it is found that the weight of each group mouse has together
Etc. degree growth, illustrate that the influence to the weight of mouse has no significant difference (p > 0.05), this guarantees mouse in biochemistry
It avoids influencing to brought by weight differences when index determining.
2. oyster composite function food of table to mouse weight influence (n=10,)
(2) influence of the oyster composite function food to mouse swimming time
Swimming time performance is mouse movement endurance size, directly the degree of reaction fatigue, walking weight load
Longer, locomitivity is stronger.For table 3 as can be seen that after continuous gavage 30 days, functional food group mouse swimming time compares blank
Compare group leader.
3. oyster composite function food of table to mouse swimming time influence (n=10,)
Note: " * " and blank control group ratio, p < 0.05；" * * " and blank control group ratio, p < 0.01.
Fatigue refers to that organism physiology function caused by body movement cannot be maintained at normal level or cannot maintain its original
Some exercise intensities and show body movement ability decline the phenomenon that.Exercise tolerance can most direct, most objective reflection machine
The degree of fatigue of body, the length of mouse negative gravity swimming time are a kind of stabilizations and common evaluation index.The experiment table
Bright, oyster composite function food of the invention has a degree of antifatigue effect.
(3) influence of the oyster composite function food to mice serum urea nitrogen content
Table 4 can be seen that the relationship of each dosage group and mice serum urea nitrogen content after continuous gavage 30d.With blank pair
It is compared according to group, the serum urea nitrogen content of functional food group is lower than blank control group (p < 0.01).
4. oyster composite function food of table to mice serum urea nitrogen content influence (n=10,)
Note: " * " and blank control group ratio, p < 0.05；" * * " and blank control group ratio, p < 0.01.
When body carries out long-time strenuous exercise, when being not enough to maintain by the energy that glycogen provides, protein just starts
It participates in metabolism and energy is provided, can rise significantly as metabolite urea nitrogen content, oyster composite function food energy of the invention
It is enough to reduce urea nitrogen content when moving, the generation of delay fatigue state.
(4) influence of the oyster composite function food to mouse hepatic glycogen content
As can be seen from Table 5, after 30d continuous gavage, compared with blank control group, functional food group mouse hepatic glycogen
Content has extremely significant increase (p < 0.01).
5. oyster composite function food of table to mouse hepatic glycogen content influence (n=10,)
Note: " * " and blank control group ratio, p < 0.05；" * * " and blank control group ratio, p < 0.01.
When organism fatigue, intracorporal hepatic glycogen can be converted to consumption of the blood glucose to make up muscle glycogen, so hepatic glycogen
Content can reduce rapidly.Therefore the deposit content of hepatic glycogen may indicate that body can be with the generation of delayed motion fatigue.By this
Measuring illustrates that oyster composite function food of the present invention can provide enough energy supplements to body, helps moving
Resisting fatigue under state.
(5) influence of the oyster composite function food to mouse Serum lactic acid content
Before movement, mouse is in resting state, and internal lactic acid content is in reduced levels.After swimming, since mouse is in
Strenuous exercise's state needs a large amount of energy supplies, needs to convert glucose into ATP by anaerobic respiration, anaerobic respiration causes
Internal lactic acid content steeply rises, functional food group mouse lactic acid content climbing it is low with blank control group compared with extremely significant
Difference (p < 0.01)；After rest, internal lactic acid is converted into pyruvic acid under the action of lactic dehydrogenase and is further metabolized, lactic acid
Horizontal to gradually decrease again, until restoring normal level, functional food group mouse phosphagen system is greater than blank control group.Such as table 6
It is shown.
6. oyster composite function food of table to mouse Serum lactic acid content influence (n=10,)
Table2.10The effected of Oyster peptide on serum lactic acid (n=10,)
Note: " * " and blank control group ratio, p < 0.05；" * * " and blank control group ratio, p < 0.01.
Under normal circumstances, glycogen is converted to glucose by glycolysis by body, then passes through tricarboxylic acid cycle oxygenolysis
Glucose generates ATP and provides energy.Under strenuous exercise, energy provides deficiency, will generate lactic acid by anaerobic respiration.Blood
Important indicator of the lactic acid content usually as detection degree of fatigue in liquid.Oyster composite function food of the invention may be by
Increase the activity of lactic dehydrogenase to promote the metabolism of lactic acid.
To sum up, the present invention wants raw material based on oyster Gly-His-Lys, and reasonably combined in conjunction with 5 taste extracts, various raw materials play it
Unique effect, the significant effect in terms of alleviating physical fatigue.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
In the technical scope disclosed by the present invention, any changes or substitutions that can be easily thought of by anyone skilled in the art,
It should be covered by the protection scope of the present invention.
Claims (3)
1. the preparation method of a seed oyster composite function food, which is characterized in that the oyster composite function food include with
Lower raw material is made: 5~15 parts of oyster Gly-His-Lys, 1~3 part of ginseng, and 2~6 parts of Herba Epimedii, 2~6 parts of root of kirilow rhodiola, fructus lycii 2
~5 parts, 3~6 parts of Cortex Eucommiae, preparation the following steps are included:
S1. ginseng, Yin Yang Yu, root of kirilow rhodiola, fructus lycii, Cortex Eucommiae are taken according to the weight ratio and powdery is made；Powdery is made in each raw material
Process it is as follows: take dry Cortex Eucommiae, fructus lycii and root of kirilow rhodiola mixing, the water of 6 times of volumes is added, heating and refluxing extraction reaches
2h is kept after slight boiling condition, decocts and extracts 2 times, is impregnated before extracting for the first time；Merge 2 decoction liquors, filters, use Rotary Evaporators
Concentration, temperature are controlled at 50 DEG C~70 DEG C, obtain just cream, powdering is lyophilized；Dry ginseng and Herba Epimedii mixing are taken, is crushed, mistake
40 meshes, it is that the alcohol reflux that 6~10 times of mass concentrations of volume of mixture are 80% extracts 2 times that volume is added after sieving, recycling
Obtained paste is precipitated freeze-drying powdering by solvent；
S2. oyster Gly-His-Lys are prepared according to the following steps:
(1) it is beaten after taking fresh oyster cleaning to drain, the water stirring of 2 times of volumes is added；
(2) trypsase 7500U/g, flavor protease 450U/g is added, adjusting pH is 8.0, and temperature is 40 DEG C~60 DEG C, reaction
3h~4h, until supernatant is extracted in supernatant and precipitating boundary clear, centrifugation；
(3) by supernatant obtained above by ceramic membrane clean, ultrafiltration membrance filter obtain molecular weight be 300~10000Da it
Between small molecule oyster Gly-His-Lys；
(4) small molecule oyster Gly-His-Lys are concentrated by nanofiltration membrane, finally spray-dried to obtain oyster Gly-His-Lys；
S3. the oyster peptide obtained the ginseng of the obtained powdery of step S1, Yin Yang Yu, root of kirilow rhodiola, fructus lycii, Cortex Eucommiae and step S2
Powder is mixed to get composition powder according to the weight ratio；
S4. capsule or tablet is made in obtained composition powder and customary adjuvant.
2. the preparation method of oyster composite function food as described in claim 1, it is characterised in that: step S2 small molecular is male
Oyster Gly-His-Lys are concentrated into solid content up to 10% by nanofiltration membrane, are finally spray-dried, and controlling spraying inlet air temperature is 180 DEG C, out
Air temperature is 80 DEG C, obtains active oyster Gly-His-Lys, packs cryo-conservation using initial vacuum.
3. the preparation method of oyster composite function food as described in claim 1, which is characterized in that the system of capsule in step S4
Standby process are as follows: the obtained composition powder of step S3 is crossed into 80 meshes, is fabricated to softwood with the ethyl alcohol that mass concentration is 85%, 16
Mesh granulation, and it is dry at a temperature of 50 DEG C~60 DEG C, and 14 mesh sieves are packed into capsule shells to get capsule finished product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610072106.1A CN105686006B (en) | 2016-02-02 | 2016-02-02 | One seed oyster composite function food and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610072106.1A CN105686006B (en) | 2016-02-02 | 2016-02-02 | One seed oyster composite function food and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105686006A CN105686006A (en) | 2016-06-22 |
| CN105686006B true CN105686006B (en) | 2019-03-19 |
Family
ID=56230073
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610072106.1A Active CN105686006B (en) | 2016-02-02 | 2016-02-02 | One seed oyster composite function food and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105686006B (en) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106173787A (en) * | 2016-08-09 | 2016-12-07 | 渤海大学 | One seed oyster effervescent tablet |
| CN106722993A (en) * | 2016-12-23 | 2017-05-31 | 浙江新维士生物科技有限公司 | A kind of functional food composite and oral products and preparation method thereof and their application |
| CN107259409A (en) * | 2017-06-05 | 2017-10-20 | 福建省水产研究所 | Health food containing the pitch-dark pigment of siphonopods and preparation method thereof |
| CN107691760A (en) * | 2017-09-21 | 2018-02-16 | 青岛海洋生物医药研究院股份有限公司 | A kind of oyster peptide of enhancing development and its preparation method and application |
| CN108244643B (en) * | 2017-12-13 | 2021-06-04 | 北京康比特体育科技股份有限公司 | Composition capable of controlling weight and improving athletic ability |
| CN107997184A (en) * | 2017-12-13 | 2018-05-08 | 国药肽谷有限公司 | The preparation method of one seed oyster oligopeptide product |
| CN109953319A (en) * | 2017-12-25 | 2019-07-02 | 山东御膳世家生物科技有限公司 | A kind of oyster peptide nutrient food and preparation method thereof |
| CN108402373A (en) * | 2018-04-23 | 2018-08-17 | 深圳市炜鑫生物科技有限公司 | A kind of yang-tonifying health food and preparation method thereof |
| CN115644331A (en) * | 2022-11-17 | 2023-01-31 | 大连鑫玉龙海洋生物种业科技股份有限公司 | Preparation method of sea cucumber and oyster compound peptide beverage capable of effectively improving anti-fatigue function |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1768765A (en) * | 2005-10-28 | 2006-05-10 | 大连水产学院 | Method for preparing antioxidation health-caring oyster product |
| CN101513437A (en) * | 2008-02-21 | 2009-08-26 | 中国科学院上海药物研究所 | Antifatigue Chinese pharmaceutical composition and preparation method thereof |
| CN102090692A (en) * | 2010-09-06 | 2011-06-15 | 山东好当家海洋发展股份有限公司 | Oyster polypeptide anti-fatigue beverage and preparation method thereof |
| CN102613555A (en) * | 2012-03-30 | 2012-08-01 | 石药集团中奇制药技术（石家庄）有限公司 | Maca powder and ginseng composition and preparation method thereof |
| CN103169079A (en) * | 2013-03-04 | 2013-06-26 | 无锡市天赐康生物科技有限公司 | Health-care food with anti-fatigue and immunity-enhancing effects, and preparation method thereof |
-
2016
- 2016-02-02 CN CN201610072106.1A patent/CN105686006B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1768765A (en) * | 2005-10-28 | 2006-05-10 | 大连水产学院 | Method for preparing antioxidation health-caring oyster product |
| CN101513437A (en) * | 2008-02-21 | 2009-08-26 | 中国科学院上海药物研究所 | Antifatigue Chinese pharmaceutical composition and preparation method thereof |
| CN102090692A (en) * | 2010-09-06 | 2011-06-15 | 山东好当家海洋发展股份有限公司 | Oyster polypeptide anti-fatigue beverage and preparation method thereof |
| CN102613555A (en) * | 2012-03-30 | 2012-08-01 | 石药集团中奇制药技术（石家庄）有限公司 | Maca powder and ginseng composition and preparation method thereof |
| CN103169079A (en) * | 2013-03-04 | 2013-06-26 | 无锡市天赐康生物科技有限公司 | Health-care food with anti-fatigue and immunity-enhancing effects, and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105686006A (en) | 2016-06-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105686006B (en) | One seed oyster composite function food and preparation method thereof | |
| CN101491334B (en) | Health food with immunity reinforcement function | |
| CN102048210B (en) | Red-date health protection beverage with function of alleviating fatigue | |
| CN102308988A (en) | Dendrobium health product having function of human body immunization enhancement | |
| CN102197878B (en) | Functional beverage | |
| CN104082658A (en) | Instant food for lowering high blood sugar, high blood pressure and high blood lipids and strengthening yang | |
| CN104322617A (en) | Biscuit assisting in regulating blood sugar level and preparation method thereof | |
| CN102114112A (en) | Effervescent tablet for improving immunity and preparation method thereof | |
| CN101142975A (en) | Rhodiola root and honey composition and preparation method thereof | |
| CN105454576A (en) | Jasmine-flower-comprising assorted Chinese herbal tea and preparation method thereof | |
| CN107212401A (en) | Composition for relieving physical fatigue and its preparation and preparation method | |
| CN109329834A (en) | A kind of fructus lycii fatigue resistant health food and preparation method thereof | |
| KR100947278B1 (en) | A carbohydrate and lipid absorption inhibitory nelumbo composition and its manufacturing process thereof | |
| CN103689152A (en) | Herbal tea for tonifying kidney and removing heat from liver and preparation method thereof | |
| CN1235601C (en) | Extractive of ocean star worm, its preparing method and application | |
| CN101401651A (en) | Culinary Chinese medicinal composition | |
| CN102960513A (en) | Healthy herbal tea and preparation method thereof | |
| CN109527577A (en) | A kind of composition with sobering up and liver protecting functions | |
| CN101574509A (en) | Agerasia preparation | |
| CN101143203B (en) | Compound oral liquid with liver-protecting and stomach-nourishing function | |
| CN101530210B (en) | Health preserving wine | |
| JP2004321087A (en) | Healthy food and method for producing the same | |
| CN111296599A (en) | Traditional Chinese medicine health tea with auxiliary blood sugar reducing effect and preparation method thereof | |
| CN109646665A (en) | Cortex Eucommiae clearing lung-heat anticancer solid tea and preparation method thereof | |
| CN106361978A (en) | Oral solution capable of relieving physical fatigue and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |