CN105685708A - Compound alfalfa herbal functional beverage and preparation method thereof - Google Patents
Compound alfalfa herbal functional beverage and preparation method thereof Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
- A23L2/04—Extraction of juices
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a compound alfalfa herbal functional beverage.The compound alfalfa herbal functional beverage is prepared from, by weight, 40-50 parts of alfalfa, 12-16 parts of stevia rebaudiana, 5-8 parts of fructus crataegi, 2-5 parts of aloe, 3-6 parts of fructus lycii, 1-4 parts of rhizoma dioscoreae and 150-200 parts of water.By adoption of whole alfalfa herbs as raw materials, alfalfa saponins, alfalfa amino acids and alfalfa polysaccharides are obtained by extraction, content of intrinsic nutrients of the raw materials is increased, and quality and nutrition of a final product are improved greatly.The prepared functional beverage has the advantages of high content of various essential amino acids for human bodies, freeness of additives due to purely natural extraction, improvement of physical functions and body immunity and efficacies of reduction of blood sugar and blood lipid and remarkable improvement of cardio-cerebral vessels.
Description
Technical field
The invention belongs to development of agricultural products technical field, be specifically related to a kind of Herba Medicaginis herbal compound drinks。
Background technology
The stem of Herba Medicaginis, leaf contain nutrient and the UGF of more than 50 kinds that include mineral nutrient, various nutritional labelings are enriched many than other plant, it is referred to as omnipotent plant, since ancient times, as improving the best herbage of oxen and horses body constitution and noticeable。Traditional Herba Medicaginis utilizes in structure, based on as feed stripped, and as natural health food application seldom。If using Herba Medicaginis as main material production Herba Medicaginis juice beverage, both can supplement the nutrients, maintain human nutrition balance, health can be promoted again, strengthen human body immunity。So Herba Medicaginis juice beverage is the another novel approach that Herba Medicaginis develops, it it is a kind of novel natural health drink。
Herba Medicaginis is with protein, and crude fiber content is high and famous, and aminoacid ratio of components relatively balances, and utilization rate is high, is the feed stripped of high-quality。Possibly together with nutritional labelings such as dietary fiber, immunocompetence polysaccharide, vitamin, calcium, phosphorus in Herba Medicaginis, add the content that can improve the nutrient substance such as Protein in Food and dietary fiber in food。
Saponin (saponin) has another name called Saponin, is the compound that a class is more complicated, owing to producing a large amount of persistency reticulated foams when its aqueous solution vibrates, similar to soap, therefore is referred to as Saponin。Alfalfa saponin has the cholesterol reduced in blood, atherosclerosis, the physiological action such as antibacterial and pest-resistant。The method extracting Saponin is more, is that the dissolubility according to Saponin is extracted mostly。The extracting method of Saponin currently mainly has following several: n-butanol extraction method, methanol or ethanol extraction acetone or ether sedimentation method and solid phase extraction, also there is employing supercritical ultrasonics technology in recent years to extract Saponin, the leaching that ultrasonic wave extraction accelerates effective ingredients in plant mainly by hyperacoustic cavitation is extracted, the advantage with time-saving energy-saving, current ultrasonic wave extraction is widely used in Chinese herbal medicine quality analysis and a small amount of, extracting, large-scale production is also little, needs to explore further。
Summary of the invention
It is an object of the invention to provide a kind of Herba Medicaginis herbal compound drinks, be a kind of novel natural health drink, be the new way of Herba Medicaginis exploitation, it is possible to significantly improve immunity of organisms, cholesterol reducing and blood fat reducing and improve effect of microcirculation in human body。
For achieving the above object, the present invention is by the following technical solutions:
A kind of Herba Medicaginis herbal compound drinks, it is made up of the raw material of following portions by weight: honeysuckle 20 ~ 50 parts, 20 ~ 50 parts of Herba Medicaginis leaf, Herba Medicaginis stem 20 ~ 50 parts, Folium Stevlae Rebaudianae 12 ~ 16 parts, Fructus Crataegi 5 ~ 8 parts, Aloe 2 ~ 5 parts, Fructus Lycii 3 ~ 6 parts, Rhizoma Dioscoreae 1 ~ 4 part, 150 ~ 200 parts of water。
Described Herba Medicaginis is the alfalfa of initial bloom stage, and honeysuckle, Herba Medicaginis leaf and Herba Medicaginis stem are butt。
The preparation method of described Herba Medicaginis herbal compound drinks, comprises the following steps:
(1) after the alfalfa of initial bloom stage being gathered in, natural air drying after the flower of alfalfa, stem and leaf are collected respectively, pulverized 50 ~ 80 mesh sieves after air-dry;
(2) method of ethanol extraction is adopted to obtain Alfalfa Polysaccharide extractum in the flower of alfalfa respectively by formula ratio, the method that the stem of alfalfa adopts microwave extraction obtains alfalfa saponin extractum, adopts alkali to carry on the leaf of alfalfa, the method for precipitate with ethanol and protease hydrolysis obtains Herba Medicaginis aminoacid extractum;
(3) Folium Stevlae Rebaudianae, Fructus Crataegi, Aloe, Fructus Lycii and Rhizoma Dioscoreae being crushed by formula ratio, the mixture obtained after being subsequently adding the water making beating of formula ratio, boil 20 ~ 25 minutes, gained boils thing and is performing centrifugal separation on, it is thus achieved that supernatant and residue;
(4) Alfalfa Polysaccharide extractum, alfalfa saponin extractum and Herba Medicaginis aminoacid extractum step (2) obtained adds in the supernatant obtained after step (3) separates, and then carries out homogenizing in homogenizer and obtains Herba Medicaginis herbal compound drinks crude product;
(5) by degassed for Herba Medicaginis herbal compound drinks crude product degasser, vacuum filling machine fill, capping, sterilization obtain finished beverage。
In described step (2), the preparation method of Alfalfa Polysaccharide extractum is as follows: be that 1:4 ~ 5 add in extraction pot by the flower of alfalfa after pulverizing and ethanol according to mass ratio, the reflux, extract, 1 ~ 2 hour when 60 ~ 70 DEG C, taking the supernatant after staticly settling, it is 1.2 ~ 1.5g/mL that the supernatant is concentrated into density。
In described step (2), the preparation method of alfalfa saponin extractum is as follows: be that 1:2 ~ 3 mixing is placed in microwave treater by the stem of alfalfa after pulverizing and ethanol according to mass ratio, microwave treater power is 300w, 20 ~ 30min is extracted when 40 ~ 60 DEG C, taking the supernatant after staticly settling, it is 1.2 ~ 1.5g/mL that the supernatant is concentrated into density。
In described step (2), the preparation method of Herba Medicaginis aminoacid extractum is as follows: after the leaf of the alfalfa after pulverizing and the mixing of NaOH solution 1:2 ~ 3 in mass ratio that concentration is 0.5 ~ 2mol/L, heat to 65 ~ 75 DEG C of extractions 2 ~ 3 hours, the supernatant is taken after being subsequently adding ethanol precipitation, adding 1 ~ 2g protease hydrolyzed 1 ~ 2 hour in every 100mL supernatant, being concentrated into density after adopting ultrafiltration membrance filter is 1.2 ~ 1.5g/mL。
Described ethanol is volume fraction is the ethanol of 80 ~ 85%。
Sterilization in described step (5) is to sterilize 3 ~ 5 minutes when temperature is 121 DEG C。
Beneficial effects of the present invention: (1) present invention adopts the method for microwave extraction fully to be extracted by the saponin in Herba Medicaginis stem, microwave-aided synthesis improves a kind of mode of extraction rate by destroying cell wall and intercellular substance to reduce composition leach resistance, penetrate frequency electromagnetic waves by good fortune and penetrate spe medium, arrive inside vascular bundle and glandular cell's system of Herba Medicaginis stem, under the impact of microwave energy, cell interior temperature rises rapidly, pressure increases, cell wall ability to bear has been exceeded once pressure, cell will break, functional component freely flows out from the cell broken, then it is extracted solvent to dissolve, reduce extraction time, increase substantially extracting efficiency, and the destruction that Herba Medicaginis is caused is only small and microwave extraction thing purity high, Extracting temperature is low, save the energy, safety non-pollution。(2) the present invention is directed to nutrient substance contained by Herba Medicaginis Herb, stem, leaf and emphasize particularly on different fields thus adopting diverse ways to extract respectively, thus functional materials is concentrated, can preparing the Herba Medicaginis herbal compound drinks that various effect is different, wherein Alfalfa Polysaccharide has the physiological functions such as enhancing Immune Function In Animals, infection, antitumor, blood fat reducing, radioprotective;Can there is esterification in alfalfa saponin, reduce lipids contents effectively under one's belt, blocks the human body absorption to food lipid and cholesterol, the contents level of cholesterol regulating;Herba Medicaginis aminoacid meets human body to amino acid whose demand, can strengthen matrix immunity simultaneously。(3) Fructus Crataegi appetite-stimulating indigestion-relieving, offsets meat stagnation effect especially and has prevention and cure of cardiovascular disease better simultaneously, reduces blood pressure and cholesterol, vessel softening and diuresis and sedation;(4) Rhizoma Dioscoreae has spleen reinforcing nourishing the stomach, and promote the production of body fluid lung benefiting, effect of the kidney invigorating arresting seminal emission;(5) Fructus Lycii contains the special dietary compositions such as abundant carotene, multivitamin and calcium, ferrum and aminoacid, glycine betaine, beautiful another name for Sichuan Province flavin, physalin, leukocyte activity can be strengthened, suppress fat to deposit in hepatocyte, promote that hepatocyte is newborn, there is the effect improving the aspects such as immunity of organisms, QI invigorating essence by force, nourishing the liver and kidney, slow down aging, adjustment blood fat and blood glucose;(6) Folium Stevlae Rebaudianae natural sweetener, there is the feature of high sugariness, low calorie, its sugariness is 200 ~ 300 times of sucrose, calorific value is only the 1/300 of sucrose, prove through high amount of drug experiment, often edible can the disease such as prophylaxis of hypertension, diabetes, obesity, heart disease, dental caries, stevioside also has health, it it is a kind of good health care sweetening agent, the present invention is aided with the natural material such as Fructus Crataegi, Folium Stevlae Rebaudianae and is seasoned, effectively cover its blue foreign smell taste, be maintained with the Herba Medicaginis distinctive grass pleasant local flavor of delicate fragrance itself。(7) present invention dries after being gathered in by Herba Medicaginis in good time and stores, and the Herba Medicaginis herbal compound multifunctional beverage prepared hence with the method for the present invention can realize large-scale batch production, and pure natural extracts without adding, and special taste is nutritious。
Detailed description of the invention
Embodiment 1
The Herba Medicaginis herbal compound drinks of the present embodiment, it is made up of following raw material:
Honeysuckle 20kg, Herba Medicaginis leaf 50kg, Herba Medicaginis stem 20kg, Folium Stevlae Rebaudianae 16kg, Fructus Crataegi 5 parts, Aloe 5kg, Fructus Lycii 3kg, Rhizoma Dioscoreae 4kg, water 150kg, described Herba Medicaginis is the alfalfa of initial bloom stage, and honeysuckle, Herba Medicaginis leaf and Herba Medicaginis stem are butt。
The preparation method of the Herba Medicaginis herbal compound drinks of the present embodiment, comprises the following steps:
(1) after the alfalfa of initial bloom stage being gathered in, natural air drying after the flower of alfalfa, stem and leaf are collected respectively, pulverized 50 ~ 80 mesh sieves after air-dry;
(2) method of ethanol extraction is adopted to obtain Alfalfa Polysaccharide extractum in the flower of alfalfa respectively by formula ratio, the method that the stem of alfalfa adopts microwave extraction obtains alfalfa saponin extractum, adopts alkali to carry on the leaf of alfalfa, the method for precipitate with ethanol and protease hydrolysis obtains Herba Medicaginis aminoacid extractum;Wherein,
The preparation method of Alfalfa Polysaccharide extractum is as follows: be that 1:4 adds in extraction pot by the flower of alfalfa after pulverizing and ethanol (80 ~ 85%) according to mass ratio, the reflux, extract, 2 hours when 60 DEG C, taking the supernatant after staticly settling, it is 1.2g/mL that the supernatant is concentrated into density;
The preparation method of alfalfa saponin extractum is as follows: be that 1:2 mixing is placed in microwave treater by the stem of alfalfa after pulverizing and ethanol (80 ~ 85%) according to mass ratio, microwave treater power is 300w, 30min is extracted when 40 DEG C, taking the supernatant after staticly settling, it is 1.2g/mL that the supernatant is concentrated into density;
The preparation method of Herba Medicaginis aminoacid extractum is as follows: after the leaf of the alfalfa after pulverizing and NaOH solution 1:3 in mass ratio that concentration is 0.5mol/L mixing, heat to 65 DEG C of extractions 3 hours, the supernatant is taken after being subsequently adding ethanol (80 ~ 85%) precipitation, adding 2gProtamex enzyme enzymolysis 1 hour in every 100mL supernatant, being concentrated into density after adopting ultrafiltration membrance filter is 1.2g/mL;
(3) Folium Stevlae Rebaudianae, Fructus Crataegi, Aloe, Fructus Lycii and Rhizoma Dioscoreae being crushed by formula ratio, the mixture obtained after being subsequently adding the water making beating of formula ratio, boil 20 minutes, gained boils thing and is performing centrifugal separation on, it is thus achieved that supernatant and residue;
(4) Alfalfa Polysaccharide extractum, alfalfa saponin extractum and Herba Medicaginis aminoacid extractum step (2) obtained adds in the supernatant obtained after step (3) separates, and then carries out homogenizing in homogenizer and obtains Herba Medicaginis herbal compound drinks crude product;
(5) by degassed for Herba Medicaginis herbal compound drinks crude product degasser, vacuum filling machine fill, capping, when 121 DEG C sterilization within 3 minutes, obtain finished beverage。
Embodiment 2
The Herba Medicaginis herbal compound drinks of the present embodiment, it is made up of following raw material:
Honeysuckle 50kg, Herba Medicaginis leaf 20kg, Herba Medicaginis stem 20kg, Folium Stevlae Rebaudianae 16kg, Fructus Crataegi 5 parts, Aloe 5kg, Fructus Lycii 3kg, Rhizoma Dioscoreae 4kg, water 150kg, described Herba Medicaginis is the alfalfa of initial bloom stage, and honeysuckle, Herba Medicaginis leaf and Herba Medicaginis stem are butt。
The preparation method of the Herba Medicaginis herbal compound drinks of the present embodiment, comprises the following steps:
(1) after the alfalfa of initial bloom stage being gathered in, natural air drying after the flower of alfalfa, stem and leaf are collected respectively, pulverized 50 ~ 80 mesh sieves after air-dry;
(2) method of ethanol extraction is adopted to obtain Alfalfa Polysaccharide extractum in the flower of alfalfa respectively by formula ratio, the method that the stem of alfalfa adopts microwave extraction obtains alfalfa saponin extractum, adopts alkali to carry on the leaf of alfalfa, the method for precipitate with ethanol and protease hydrolysis obtains Herba Medicaginis aminoacid extractum;Wherein,
The preparation method of Alfalfa Polysaccharide extractum is as follows: be that 1:5 adds in extraction pot by the flower of alfalfa after pulverizing and ethanol (80 ~ 85%) according to mass ratio, the reflux, extract, 1 hour when 70 DEG C, taking the supernatant after staticly settling, it is 1.5g/mL that the supernatant is concentrated into density。
The preparation method of alfalfa saponin extractum is as follows: be that 1:3 mixing is placed in microwave treater by the stem of alfalfa after pulverizing and ethanol (80 ~ 85%) according to mass ratio, microwave treater power is 300w, 20min is extracted when 60 DEG C, taking the supernatant after staticly settling, it is 1.5g/mL that the supernatant is concentrated into density;
The preparation method of Herba Medicaginis aminoacid extractum is as follows: after the leaf of the alfalfa after pulverizing and NaOH solution 1:3 in mass ratio that concentration is 2mol/L mixing, heat to 75 DEG C of extractions 2 hours, the supernatant is taken after being subsequently adding ethanol (80 ~ 85%) precipitation, adding 1gProtamex enzyme enzymolysis 2 hours in every 100mL supernatant, being concentrated into density after adopting ultrafiltration membrance filter is 1.5g/mL;
(3) Folium Stevlae Rebaudianae, Fructus Crataegi, Aloe, Fructus Lycii and Rhizoma Dioscoreae being crushed by formula ratio, the mixture obtained after being subsequently adding the water making beating of formula ratio, boil 25 minutes, gained boils thing and is performing centrifugal separation on, it is thus achieved that supernatant and residue;
(4) Alfalfa Polysaccharide extractum, alfalfa saponin extractum and Herba Medicaginis aminoacid extractum step (2) obtained adds in the supernatant obtained after step (3) separates, and then carries out homogenizing in homogenizer and obtains Herba Medicaginis herbal compound drinks crude product;
(5) by degassed for Herba Medicaginis herbal compound drinks crude product degasser, vacuum filling machine fill, capping, when 121 DEG C sterilization within 5 minutes, obtain finished beverage。
Embodiment 3
The Herba Medicaginis herbal compound drinks of the present embodiment, it is made up of following raw material:
Honeysuckle 30kg, Herba Medicaginis leaf 40kg, Herba Medicaginis stem 50kg, Folium Stevlae Rebaudianae 14kg, Fructus Crataegi 6 parts, Aloe 3kg, Fructus Lycii 4kg, Rhizoma Dioscoreae 2kg, water 180kg, described Herba Medicaginis is the alfalfa of initial bloom stage, and honeysuckle, Herba Medicaginis leaf and Herba Medicaginis stem are butt。
The preparation method of the Herba Medicaginis herbal compound drinks of the present embodiment, comprises the following steps:
(1) after the alfalfa of initial bloom stage being gathered in, natural air drying after the flower of alfalfa, stem and leaf are collected respectively, pulverized 50 ~ 80 mesh sieves after air-dry;
(2) method of ethanol extraction is adopted to obtain Alfalfa Polysaccharide extractum in the flower of alfalfa respectively by formula ratio, the method that the stem of alfalfa adopts microwave extraction obtains alfalfa saponin extractum, adopts alkali to carry on the leaf of alfalfa, the method for precipitate with ethanol and protease hydrolysis obtains Herba Medicaginis aminoacid extractum;Wherein,
The preparation method of Alfalfa Polysaccharide extractum is as follows: be that 1:5 adds in extraction pot by the flower of alfalfa after pulverizing and ethanol (80 ~ 85%) according to mass ratio, the reflux, extract, 1.5 hours when 65 DEG C, taking the supernatant after staticly settling, it is 1.4g/mL that the supernatant is concentrated into density;
The preparation method of alfalfa saponin extractum is as follows: be that 1:2.5 mixing is placed in microwave treater by the stem of alfalfa after pulverizing and ethanol (80 ~ 85%) according to mass ratio, microwave treater power is 300w, 25min is extracted when 50 DEG C, taking the supernatant after staticly settling, it is 1.4g/mL that the supernatant is concentrated into density;
The preparation method of Herba Medicaginis aminoacid extractum is as follows: after the leaf of the alfalfa after pulverizing and NaOH solution 1:2.5 in mass ratio that concentration is 1mol/L mixing, heat to 70 DEG C of extractions 2.5 hours, the supernatant is taken after being subsequently adding ethanol (80 ~ 85%) precipitation, adding 1.5gProtamex enzyme enzymolysis 1.5 hours in every 100mL supernatant, being concentrated into density after adopting ultrafiltration membrance filter is 1.2 ~ 1.5g/mL;
(3) Folium Stevlae Rebaudianae, Fructus Crataegi, Aloe, Fructus Lycii and Rhizoma Dioscoreae being crushed by formula ratio, the mixture obtained after being subsequently adding the water making beating of formula ratio, boil 24 minutes, gained boils thing and is performing centrifugal separation on, it is thus achieved that supernatant and residue;
(4) Alfalfa Polysaccharide extractum, alfalfa saponin extractum and Herba Medicaginis aminoacid extractum step (2) obtained adds in the supernatant obtained after step (3) separates, and then carries out homogenizing in homogenizer and obtains Herba Medicaginis herbal compound drinks crude product;
(5) by degassed for Herba Medicaginis herbal compound drinks crude product degasser, vacuum filling machine fill, capping, when 121 DEG C sterilization within 4 minutes, obtain finished beverage。
Embodiment 4
The Herba Medicaginis herbal compound drinks of the present embodiment, it is made up of following raw material:
Honeysuckle 40kg, Herba Medicaginis leaf 25kg, Herba Medicaginis stem 35kg, Folium Stevlae Rebaudianae 15kg, Fructus Crataegi 7 parts, Aloe 4kg, Fructus Lycii 5kg, Rhizoma Dioscoreae 3kg, water 160kg, described Herba Medicaginis is the alfalfa of initial bloom stage, and honeysuckle, Herba Medicaginis leaf and Herba Medicaginis stem are butt。
The preparation method of the Herba Medicaginis herbal compound drinks of the present embodiment, comprises the following steps:
(1) after the alfalfa of initial bloom stage being gathered in, natural air drying after the flower of alfalfa, stem and leaf are collected respectively, pulverized 50 ~ 80 mesh sieves after air-dry;
(2) method of ethanol extraction is adopted to obtain Alfalfa Polysaccharide extractum in the flower of alfalfa respectively by formula ratio, the method that the stem of alfalfa adopts microwave extraction obtains alfalfa saponin extractum, adopts alkali to carry on the leaf of alfalfa, the method for precipitate with ethanol and protease hydrolysis obtains Herba Medicaginis aminoacid extractum;Wherein,
The preparation method of Alfalfa Polysaccharide extractum is as follows: be that 1:5 adds in extraction pot by the flower of alfalfa after pulverizing and ethanol (80 ~ 85%) according to mass ratio, the reflux, extract, 2 hours when 60 DEG C, taking the supernatant after staticly settling, it is 1.3g/mL that the supernatant is concentrated into density;
The preparation method of alfalfa saponin extractum is as follows: be that 1:3 mixing is placed in microwave treater by the stem of alfalfa after pulverizing and ethanol (80 ~ 85%) according to mass ratio, microwave treater power is 300w, 30min is extracted when 40 DEG C, taking the supernatant after staticly settling, it is 1.3g/mL that the supernatant is concentrated into density;
The preparation method of Herba Medicaginis aminoacid extractum is as follows: after the leaf of the alfalfa after pulverizing and NaOH solution 1:3 in mass ratio that concentration is 1.5mol/L mixing, heat to 65 DEG C of extractions 3 hours, the supernatant is taken after being subsequently adding ethanol (80 ~ 85%) precipitation, adding 2gProtamex enzyme enzymolysis 2 hours in every 100mL supernatant, being concentrated into density after adopting ultrafiltration membrance filter is 1.4g/mL;
(3) Folium Stevlae Rebaudianae, Fructus Crataegi, Aloe, Fructus Lycii and Rhizoma Dioscoreae being crushed by formula ratio, the mixture obtained after being subsequently adding the water making beating of formula ratio, boil 24 minutes, gained boils thing and is performing centrifugal separation on, it is thus achieved that supernatant and residue;
(4) Alfalfa Polysaccharide extractum, alfalfa saponin extractum and Herba Medicaginis aminoacid extractum step (2) obtained adds in the supernatant obtained after step (3) separates, and then carries out homogenizing in homogenizer and obtains Herba Medicaginis herbal compound drinks crude product;
(5) by degassed for Herba Medicaginis herbal compound drinks crude product degasser, vacuum filling machine fill, capping, when 121 DEG C sterilization within 4 minutes, obtain finished beverage。
The ultimate principle of the present invention and principal character and advantages of the present invention have more than been shown and described。Skilled person will appreciate that of the industry; the present invention is not restricted to the described embodiments; described in above-described embodiment and description is that principles of the invention is described; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements both fall within the claimed scope of the invention。Claimed scope is defined by appending claims and equivalent thereof。
Claims (8)
1. a Herba Medicaginis herbal compound drinks, it is characterised in that: it is made up of the raw material of following portions by weight: honeysuckle 20 ~ 50 parts, 20 ~ 50 parts of Herba Medicaginis leaf, Herba Medicaginis stem 20 ~ 50 parts, Folium Stevlae Rebaudianae 12 ~ 16 parts, Fructus Crataegi 5 ~ 8 parts, Aloe 2 ~ 5 parts, Fructus Lycii 3 ~ 6 parts, Rhizoma Dioscoreae 1 ~ 4 part, 150 ~ 200 parts of water。
2. Herba Medicaginis herbal compound drinks according to claim 1, it is characterised in that: described Herba Medicaginis is the alfalfa of initial bloom stage, and honeysuckle, Herba Medicaginis leaf and Herba Medicaginis stem are butt。
3. the preparation method of Herba Medicaginis herbal compound drinks according to claim 2, it is characterised in that comprise the following steps:
(1) after the alfalfa of initial bloom stage being gathered in, natural air drying after the flower of alfalfa, stem and leaf are collected respectively, pulverized 50 ~ 80 mesh sieves after air-dry;
(2) flower to alfalfa is divided to adopt the method for ethanol extraction to obtain Alfalfa Polysaccharide extractum by formula ratio, the method that the stem of alfalfa adopts microwave extraction obtains alfalfa saponin extractum, adopts alkali to carry on the leaf of alfalfa, the method for precipitate with ethanol and protease hydrolysis obtains Herba Medicaginis aminoacid extractum;
(3) Folium Stevlae Rebaudianae, Fructus Crataegi, Aloe, Fructus Lycii and Rhizoma Dioscoreae being crushed by formula ratio, the mixture obtained after being subsequently adding the water making beating of formula ratio, boil 20 ~ 25 minutes, gained boils thing and is performing centrifugal separation on, it is thus achieved that supernatant and residue;
(4) Alfalfa Polysaccharide extractum, alfalfa saponin extractum and Herba Medicaginis aminoacid extractum step (2) obtained adds in the supernatant obtained after step (3) separates, and then carries out homogenizing in homogenizer and obtains Herba Medicaginis herbal compound drinks crude product;
(5) by degassed for Herba Medicaginis herbal compound drinks crude product degasser, vacuum filling machine fill, capping, sterilization obtain finished beverage。
4. the preparation method of Herba Medicaginis herbal compound drinks according to claim 3, it is characterized in that: in described step (2), the preparation method of Alfalfa Polysaccharide extractum is as follows: be that 1:4 ~ 5 add in extraction pot by the flower of alfalfa after pulverizing and ethanol according to mass ratio, the reflux, extract, 1 ~ 2 hour when 60 ~ 70 DEG C, taking the supernatant after staticly settling, it is 1.2 ~ 1.5g/mL that the supernatant is concentrated into density。
5. the preparation method of Herba Medicaginis herbal compound drinks according to claim 3, it is characterized in that: in described step (2), the preparation method of alfalfa saponin extractum is as follows: be that 1:2 ~ 3 mixing is placed in microwave treater by the stem of alfalfa after pulverizing and ethanol according to mass ratio, microwave treater power is 300w, 20 ~ 30min is extracted when 40 ~ 60 DEG C, taking the supernatant after staticly settling, it is 1.2 ~ 1.5g/mL that the supernatant is concentrated into density。
6. the preparation method of Herba Medicaginis herbal compound drinks according to claim 3, it is characterised in that:
In described step (2), the preparation method of Herba Medicaginis aminoacid extractum is as follows: after the leaf of the alfalfa after pulverizing and the mixing of NaOH solution 1:2 ~ 3 in mass ratio that concentration is 0.5 ~ 2mol/L, heat to 65 ~ 75 DEG C of extractions 2 ~ 3 hours, the supernatant is taken after being subsequently adding ethanol precipitation, adding 1 ~ 2g protease hydrolyzed 1 ~ 2 hour in every 100mL supernatant, being concentrated into density after adopting ultrafiltration membrance filter is 1.2 ~ 1.5g/mL。
7. the preparation method of the Herba Medicaginis herbal compound drinks according to claim 4 or 5 or 6, it is characterised in that: described ethanol is volume fraction is the ethanol of 80 ~ 85%。
8. the preparation method of Herba Medicaginis herbal compound drinks according to claim 3, it is characterised in that: the sterilization in described step (5) is to sterilize 3 ~ 5 minutes when temperature is 121 DEG C。
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