CN105675460A - Method for accelerating blood sedimentation by virtue of voltage - Google Patents
Method for accelerating blood sedimentation by virtue of voltage Download PDFInfo
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- CN105675460A CN105675460A CN201610130330.1A CN201610130330A CN105675460A CN 105675460 A CN105675460 A CN 105675460A CN 201610130330 A CN201610130330 A CN 201610130330A CN 105675460 A CN105675460 A CN 105675460A
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- blood
- sedimentation rate
- erythrocyte sedimentation
- voltage
- electric field
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/04—Investigating sedimentation of particle suspensions
- G01N15/05—Investigating sedimentation of particle suspensions in blood
Abstract
The invention discloses a method for accelerating blood sedimentation by virtue of voltage. The method comprises the steps of mixing blood with an anticoagulant, injecting the mixture into a blood sedimentation pipe, vertically arranging the blood sedimentation pipe to a test frame, and applying voltage to the upper and lower ends of the blood sedimentation pipe, so as to accelerate the downward sedimentation of electronegative red blood cells in blood by virtue of an electric field action force, wherein the upper end of the blood sedimentation pipe is a cathode, and the lower end of the blood sedimentation pipe is an anode. According to the method, the voltage is applied to the upper and lower ends of the blood sedimentation pipe to form the electric field, and the electronegative red blood cells in the electric field are influenced by the downward electric field action force similar to a centrifugal force, so that the sedimentation of the red blood cells can be accelerated, and the rapid detection is realized. The detection method is simple and low in cost. Compared with an existing full-automatic erythrocyte sediment rate analyzer, a detection device designed according to the method has the advantages that moving parts are greatly reduced, the work is reliable, and the maintenance is easy.
Description
Technical field
The present invention relates to medical science detection, specifically refer to a kind of method utilizing voltage to accelerate erythrocyte sedimentation rate, belong to medical treatment detection and medical equipment technical field.
Background technology
Erythrocyte sedimentation rate (ESR) i.e. erythrocyte sedimentation rate, though the diagnosis and treatment of disease are lacked specificity by erythrocyte sedimentation rate detection, but it is to the generation of infectious and autoimmune disorder, development, and observation of curative effect and Index for diagnosis are still significant, are still one of conventional Clinical laboratory test so far.
Erythrocyte sedimentation rate principle is the factors such as the negative charge that has of the saliva on red cell membrane surface in blood flow and mutually exclusive makes cell spacing be about 25nm, higher than blood plasma containing protein content, proportion is greater than blood plasma, and double intended circle plate-like makes it by certain blood plasma reverse blocking power, thus each other dispersion suspension and sink slowly. If blood plasma or red corpuscle itself change, then erythrocyte sedimentation rate can be made to change. Red corpuscle sinks to dividing a three phases: the 1. red corpuscle string for stringing up cash in ancient times money shape accumulative phase: " disc plane " of red corpuscle is fitted each other and formed red corpuscle string for stringing up cash in ancient times cash strings, namely two red corpuscle laminatings eliminate two " disc plane ", often increase the red corpuscle of a laminating on this basis, i.e. many eliminations two " disc plane ". This process about needs 10min; 2. the red corpuscle rapid subsidence phase: red corpuscle bonded to each other increases gradually, sinking velocity is accelerated, and this stage about continues 40min; 3. red corpuscle piles up the phase: this phase erythrocyte number of laminating reaches capacity and slowly declines, tightly packed and container bottom.
The esr detection method that the international hematology stdn council (ICSH) is recommended is westergren method, but westergren method length consuming time, experimental factors is many, condition is not easily grasped unanimously, and it is not easy to laboratory streamline operration, it is difficult to meet clinical emergency treatment testing requirement, is substantially replaced by full-automatic esr analyzer at present. Full-automatic esr analyzer adopts EDTA-K2 anticoagulant tube to be placed in and detects with on the test-tube stand on automatic mixing device, instrument blocks a shot puncture suction Trace Blood automatically in kapillary, after centrifugal, detect under 37 DEG C of constant temperature, with noctovisor scan, changed by detection of dynamic erythrocyte aggregation and sedimentation, it is converted into the result relevant to westergren method with optical density(OD) change. Full-automatic esr analyzer makes red corpuscle move downward based on centrifugal force, bottom erythrocyte sedimentation rate pipe, due to the stop of tube wall, red corpuscle string for stringing up cash in ancient times money shape aggregation velocity is accelerated, and then weaken the mutual repulsion to upper strata red corpuscle, thus accelerate red corpuscle and sink and pile up at the bottom of erythrocyte sedimentation rate pipe, therefore can accelerate esr detection speed. Full-automatic esr analyzer accelerates erythrocyte sedimentation based on centrifugal principle, therefore corresponding accessory motor, moving parts are more, and energy expenditure is relatively big, and failure rate is also high. Therefore, this area testing staff is finding the erythrocyte sedimentation rate detection method of a kind of efficient, economic and system stability newly always.
Summary of the invention
For prior art above shortcomings, the voltage that utilizes it is an object of the invention to propose a kind of to detect simple, reliable operation, being easy to safeguard, cost is low accelerates the method for erythrocyte sedimentation rate.
The technical scheme of the present invention is achieved in that
A kind of method utilizing voltage to accelerate erythrocyte sedimentation rate, erythrocyte sedimentation rate pipe is injected after blood and antithrombotics being mixed, again erythrocyte sedimentation rate pipe is vertically placed on testing jig, voltage is applied at the upper and lower go-and-retum of erythrocyte sedimentation rate pipe, wherein erythrocyte sedimentation rate pipe upper end is negative pole, lower end is positive pole, utilizes electric field action power to make red corpuscle electronegative in blood accelerate settle downward.
Described applying voltage is 40～120V.
Described antithrombotics is potassium oxalate, Sodium Citrate or EDTA-K2.
The volume ratio of described blood and antithrombotics is 1:1～1:4.
Described erythrocyte sedimentation rate pipe internal diameter is 1.0～1.8mm.
The present invention applies voltage up and down at erythrocyte sedimentation rate pipe, forms electric field, and the red corpuscle being arranged in electric field is electronegative because of it, is subject to the electric field action power that similar centrifugal force is equally downward, therefore can accelerate erythrocyte sedimentation, makes detection quick.
Compared to existing technology, the present invention has following useful effect:
1, detection method is simple, and only need to apply voltage can accelerate sedimentation, it is achieved efficient esr detection.
2, the detection device detecting thought design according to the present invention is compared with existing full-automatic esr analyzer, and movable parts reduce in a large number, reliable operation, are easy to safeguard.
3, the present invention adopts electric field action in red corpuscle, the same realizes efficient esr detection with centrifugal, and cost is low.
Accompanying drawing explanation
Fig. 1-principle of the invention schematic diagram.
Embodiment
Owing to electronegative red corpuscle can be formed reactive force by electric field, namely electric field can accelerate esr detection as centrifugal, and therefore, the present invention utilizes red corpuscle electronegative, can move to positive pole fast under electric field action.
When specifically operating, injecting erythrocyte sedimentation rate pipe after first blood and antithrombotics being mixed, erythrocyte sedimentation rate pipe internal diameter is the volume ratio of 1.0～1.8mm, blood and antithrombotics is 1:1～1:4; Vertically being placed on testing jig by erythrocyte sedimentation rate pipe, apply voltage at the upper and lower go-and-retum of erythrocyte sedimentation rate pipe, voltage is 40～120V, and wherein erythrocyte sedimentation rate pipe upper end is negative pole, and lower end is positive pole, utilizes electric field action power to make red corpuscle electronegative in blood accelerate settle downward.
Below in conjunction with drawings and Examples, the present invention is described in further detail.
The principle accelerating erythrocyte sedimentation in erythrocyte sedimentation rate of the present invention detection is as shown in Figure 1, as can be seen from figure is upper, it forms primarily of erythrocyte sedimentation rate pipe 1, the blood being positioned at erythrocyte sedimentation rate pipe and antithrombotics mixed solution 2, two battery lead plate 3, photorectifier 4 and direct supply 5, erythrocyte sedimentation rate Guan Shuxiang arrange and between two battery lead plates 3. Two battery lead plates 3 connect power positive cathode, and the battery lead plate 3 being wherein positioned at erythrocyte sedimentation rate pipe upper end connects power cathode, and the battery lead plate 3 being positioned at the lower end of erythrocyte sedimentation rate pipe connects positive source. Like this under electrical forces effect, electronegative red corpuscle will be accelerated to move to positive pole direction, thus improves detection efficiency.
In order to the effect of present method being described and finds out better embodiment, inventive design 6 embodiments detect. The blood that all embodiments adopt is New Zealand's daytime blood, antithrombotics is potassium oxalate, Sodium Citrate or EDTA-K2, being analytical pure, blood sampling uses medical vacuum heparin tube, and applying voltage source is aobvious doubleway output DC voltage-stabilizing power supply (APS3003S30V3A) of the farsighted safe and sound letter number of state.
Component concentration in each embodiment and processing mode are as shown in table 1,2:
The each embodiment erythrocyte sedimentation rate sample of table 1
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | Embodiment 6 | |
| Potassium oxalate | 0.5ml | 2.0ml | ||||
| Sodium Citrate | 1ml | 0.5ml | ||||
| EDTA-K2 | 1.5ml | 1ml | ||||
| Blood | 0.5ml | 0.5ml | 0.5ml | 0.5ml | 0.5ml | 0.5ml |
| Blood and antithrombotics volume ratio | 1:1 | 2:1 | 3:1 | 4:1 | 1:1 | 2:1 |
The each embodiment condition of table 2
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | Embodiment 6 | |
| Erythrocyte sedimentation rate pipe internal diameter | 1.0mm | 1.5mm | 1.8mm | 1.0mm | 1.5mm | 1.8mm |
| Volts DS | 40V | 60V | 80V | 90V | 100V | 120V |
Blood in table 1 and corresponding antithrombotics are mixed in proportion by each embodiment, inject the erythrocyte sedimentation rate pipe such as table 2 correspondence, vertically it is placed on testing jig, adopting copper sheet as battery lead plate, upper and lower two ends apply the voltage such as table 2, and test environment maintains 37 ± 2 DEG C, interval 6min, adopt photorectifier detection Blood Sedimentation face height, get 18min, 1h and measure Blood Sedimentation speed, as shown in table 3.
Table 3 each embodiment Blood Sedimentation speed (mm.min-1)
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | Embodiment 6 | |
| 18min | 0.401 | 0.513 | 0.585 | 0.695 | 0.487 | 0.884 |
| 1h | 0.493 | 0.662 | 0.785 | 0.531 | 0.601 | 0.368 |
Being shown by above-described embodiment, what the present invention proposed accelerates to measure erythrocyte sedimentation rate method under electric field action is feasible. And preferred detection condition is: antithrombotics to be potassium oxalate or Sodium Citrate, blood and antithrombotics volume ratio be 1:1, voltage 80V, detection time 18min.
Finally it should be noted that, above embodiment is only in order to illustrate the technical scheme of the present invention and unrestricted, although applicant's better embodiment of reference is to invention has been detailed explanation, it will be understood by those within the art that, the technical scheme of the present invention is modified or equivalent replacement, and do not depart from objective and the scope of the technical program, all should be encompassed in the middle of the right of the present invention.
Claims (5)
1. one kind utilizes the method for voltage quickening erythrocyte sedimentation rate, it is characterized in that: after blood and antithrombotics being mixed, inject erythrocyte sedimentation rate pipe, again erythrocyte sedimentation rate pipe is vertically placed on testing jig, voltage is applied at the upper and lower go-and-retum of erythrocyte sedimentation rate pipe, wherein erythrocyte sedimentation rate pipe upper end is negative pole, lower end is positive pole, utilizes electric field action power to make red corpuscle electronegative in blood accelerate settle downward.
2. a kind of method utilizing voltage to accelerate erythrocyte sedimentation rate according to claim 1, it is characterised in that: described applying voltage is 40～120V.
3. a kind of method utilizing voltage to accelerate erythrocyte sedimentation rate according to claim 1, it is characterised in that: described antithrombotics is potassium oxalate, Sodium Citrate or EDTA-K2.
4. a kind of method utilizing voltage to accelerate erythrocyte sedimentation rate according to claim 1, it is characterised in that: the volume ratio of described blood and antithrombotics is 1:1～1:4.
5. a kind of method utilizing voltage to accelerate erythrocyte sedimentation rate according to claim 1, it is characterised in that: described erythrocyte sedimentation rate pipe internal diameter is 1.0～1.8mm.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610130330.1A CN105675460A (en) | 2016-03-08 | 2016-03-08 | Method for accelerating blood sedimentation by virtue of voltage |
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| CN201610130330.1A CN105675460A (en) | 2016-03-08 | 2016-03-08 | Method for accelerating blood sedimentation by virtue of voltage |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110456046A (en) * | 2019-08-26 | 2019-11-15 | 刘海波 | Immune rapid detection card |
| CN112033868A (en) * | 2020-09-01 | 2020-12-04 | 湖南伊鸿健康科技有限公司 | Method for accelerating cell precipitation, metal plate and microscope |
| CN112557267A (en) * | 2020-12-25 | 2021-03-26 | 浙江师范大学 | Blood sedimentation detection device and detection method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110456046A (en) * | 2019-08-26 | 2019-11-15 | 刘海波 | Immune rapid detection card |
| CN112033868A (en) * | 2020-09-01 | 2020-12-04 | 湖南伊鸿健康科技有限公司 | Method for accelerating cell precipitation, metal plate and microscope |
| CN112557267A (en) * | 2020-12-25 | 2021-03-26 | 浙江师范大学 | Blood sedimentation detection device and detection method thereof |
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