CN105671090A - Method for producing ethanol by carrying out high temperature enzymolysis and fermentation on lignocellulose - Google Patents
Method for producing ethanol by carrying out high temperature enzymolysis and fermentation on lignocellulose Download PDFInfo
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
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- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
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- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
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- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
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Abstract
The invention discloses a method for producing ethanol by carrying out high temperature enzymolysis and fermentation on lignocellulose. The method for producing the ethanol by carrying out the high temperature enzymolysis and fermentation on the lignocellulose comprises the following steps: (1) pretreating a lignocellulose raw material, so as to obtain a pretreated raw material; (2) adding the pretreated raw material, cellulase, water and xanthan gum into an enzymatic vessel for carrying out pre-enzymolysis, and controlling dry material concentration of an enzymolysis system to be 15-25wt%; (3) pumping an enzymolyzed feed liquid into a fermentation tank, adding temperature-resistant saccharomyces cerevisiae, and carrying out simultaneous saccharification and fermentation; and (4) pumping fermentation liquor into a rectification device and separating ethanol. The method disclosed by the invention has the advantages that the xanthan gum is added into the enzymolysis system, high temperature tolerance of cellulase is improved, activity of the cellulase under high temperature enzymolysis condition is high, usage amount of the cellulase is reduced, enzymolysis efficiency of the lignocellulose is improved, and economy is improved.
Description
Technical field
The invention belongs to biomass energy source domain, a kind of method being specifically related to lignocellulose high temperature enzyme hydrolysis and fermentation producing and ethanol.
Background technology
Increasing along with world population and various countries' industrialization degree improves, energy resource consumption continues to rise. Oil is the Main Resources meeting energy demand, but petroleum resources are limited, and scientist predicts the year two thousand fifty crude output and will be dropped to 5,000,000,000 barrels by 25,800,000,000 barrels (35.25 hundred million tons) of 2009. Bioenergy is as a kind of reproducible transportation fuel, it is possible to effectively reduces room temperature effect, slows down environmental pollution, changes existing unequal oil supply and demand relation simultaneously, continues to supply, have clear superiority than mineral nitrogen fuel. Compared with other bioenergy such as biodiesel, the production of alcohol fuel has been provided with considerable scale (about 17,000,000 tons of the whole world in 2002), mainly to pass through fermentative Production containing sugar substance for raw material. The dehydrated alcohol (E10) mixing 10% in the oil is possible not only to alleviation Pressure on Energy, moreover it is possible to improves octane number, improves exhaust emissions quality. In the U.S. and Brazil, alcohol fuel has been widely used as traffic fuel as oil substitutes.
The Chinese government attaches great importance to energy diversification and problem of environmental pollution, takes the incentives such as financial subsidies and deductions and exemptions of taxes, carries forward vigorously technology and the industry development of the diversification petroleum replacing energy. The appearance of " Renewable Energy Law " and National Program for Medium-to Long-term Scientific and Technological Development, greatly advances the development process of the biological liquid fuel such as biodiesel and alcohol fuel. China is planned for the year two thousand twenty, and bio-fuel consumption figure accounts for about the 15% of whole traffic fuel, it is established that have the bio-fuel industry of international competitiveness, and this brings good opportunity to develop to China's fuel ethanol industrial. Current China ethanol petrol has covered 9 provinces and cities in the whole nation, existing alcohol fuel production capacity 1,520,000 tons, and actual production alcohol fuel more than 1,800,000 tons in 2010, with Semen Maydis and Semen Tritici aestivi for primary raw material. China has a large population and a few land, cultivated land resource is in short supply, and staple food supply is nervous, will threaten the grain security of country with Semen Maydis, Semen Tritici aestivi for raw material production alcohol fuel, cause the chain reactions such as agricultural product price rise, thus China strictly control with grain be raw material alcohol fuel newly-built and expand can project.
For saccharide and amylum crops, lignocellulose belongs to non-grain raw material, and aboundresources.It can derive from agricultural wastes, such as wheat straw, corn straw, corn cob, soybean residue, bagasse etc.; Trade waste, such as slurrying and the fibrous residue in paper mill, sawdust etc.; Forestry waste; Urban waste, such as waste paper, wrapping paper etc. Lignocellulosic material accounts for the 50% of world's Biomass 10,000,000,000-500 hundred million tons according to estimates, China with regard to agricultural crop straw annual output namely up to 700,000,000 tons, go out of use in a large number and do not add the fibre object (sprig, bark, leaves, bits and waste paper etc.) of utilization there are about 500,000,000 tons/annual, only also to produce the alcohol fuel of 20,000,000 tons per year with 100,000,000 tons therein. As can be seen here, the new technology developing Production of Alcohol from Lignocellulose has good prospect.
The cost of cellulase hydrolysis process cellulase, ethanol distillation process energy consumption, and the seriality of enzymolysis process is always up the industrialized restraining factors of cellulose ethanol. Chinese patent CN200910098877.8 discloses a kind of cellulase producing bacteria and preparation thereof and application, and this bacterial strain has resistant to elevated temperatures ability, and the cellulase of its secretion has resistant to elevated temperatures feature. Chinese patent CN201110025776.5 discloses a kind of high-temperature acidic cellulase EgG5 and gene thereof and application, and the cellulase of this invention has the property that optimum pH 3.5～5.0, optimum temperature 70 DEG C, and ratio is lived as 60.3U/mg; Good stability, still keeps high enzyme to live within the scope of Extreme acid. CN200810189465.0 discloses the production method of a kind of cellulosic ethanol, including following steps: the culture medium containing cellulose and/or hemicellulosic material is joined in fermentation reaction still by (1); (2) in fermentation reaction still, add cellulase, and inoculate Candida lusitaniae; (3) under the combined effect of cellulase and Candida lusitaniae, carry out simultaneous saccharification and fermentation, separate and obtain cellulosic ethanol.
The hydrolysis temperature that the cellulase that traditional cellulase technique adopts relatively optimizes is 45-50 DEG C, research shows that higher temperature enzymolysis can improve enzymolysis efficiency, that reduces cellulase makes consumption, existing high temperature-resisting cellulase may be used for high temperature enzyme hydrolysis and fermentation ethanol, but also immature, arrive far away industrialized degree. Develop a kind of method that conventional commercial cellulase can be made to improve hydrolysis temperature cellulosic ethanol industrial expansion is significant.
Summary of the invention
For the deficiencies in the prior art, a kind of method that the invention provides lignocellulose high temperature enzyme hydrolysis and fermentation producing and ethanol. The inventive method can improve activity and the utilization rate of enzyme, increase fermentation product yield, it is possible to decrease enzymolysis and fermentation costs.
Lignocellulose high temperature enzymolysis of the present invention and fermentation producing and ethanol method, including following content:
(1) lignocellulosic material is carried out pretreatment, it is thus achieved that pretreated feedstock;
(2) pretreated feedstock, cellulase, water and xanthan gum being joined and carry out pre-enzymolysis in enzymatic vessel, the dry substance concentration of controlled enzymatic hydrolysis system is 15wt%-25wt%, and pre-hydrolysis temperature controls at 54-62 DEG C; The time of staying of controlled enzymatic hydrolysis feed liquid is 8-48h; The pH of pre-enzymolysis is 4.5-5.5;
(3) after pre-enzymolysis, feed liquid is driven in fermentation tank, adds heatproof saccharomyces cerevisiae and carries out simultaneous saccharification and fermentation, and fermentation temperature controls at 36-42 DEG C;
(4) fermentation liquid is driven into rectifier unit separating alcohol.
In step (1), lignocellulose is preferably corn straw, and described pretreatment mode preferably employs dilute acid steam explosion combination pretreatment.
In step (2), xanthan gum addition is 0.08-0.24g/L.
In step (2), the time of staying of controlled enzymatic hydrolysis feed liquid is preferably 12-24h; The pH of pre-enzymolysis is preferably 4.8-5.2; Temperature is preferably 56-60 DEG C.
In step (2), the addition of described cellulase makes cellulase be 5-25IU/g cellulose with cellulosic ratio in pretreated feedstock.
In step (3), simultaneous saccharification and fermentation refer to pre-enzymolysis after residual cellulose at glucose fermentation producing and ethanol while continuing to the process that is hydrolyzed.
In step (3), described heatproof saccharomyces cerevisiae preferably can tolerate the heatproof saccharomyces cerevisiae of 36-42 DEG C; The inoculum concentration of heatproof alcohol fermentation bacterium seed liquor is 1v%-5v%; Being made directly simultaneous saccharification and fermentation after enzymolysis, control fermentation temperature and be 36-42 DEG C, fermentation time is 12-96h.
In step (3), adding nitrogenous source in simultaneous saccharification and fermentation system, one or more in yeast extract, peptone, Semen Maydis pulp, ammonium sulfate or carbamide of nitrogenous source, nitrogenous source addition is the 0.02wt%-0.2wt% of system gross mass.
In step (3), the preferred carbamide of nitrogenous source.
Compared with prior art, the invention have the advantages that
1, in enzymatic hydrolysis system, add xanthan gum, improve the ability of the tolerance high temperature of cellulase, improve the temperature of enzymolysis, be conducive to improving the activity of cellulase, reduce the consumption that makes of cellulase, improve the enzymolysis efficiency of lignocellulose, improve economy.
2, adopt thermotolerant yeast bacterium that cellulose remaining after the pre-enzymolysis of high temperature is carried out simultaneous saccharification and fermentation, it is possible to reduce the feedback suppression of Fructus Vitis viniferae sugar mutual-cellulose enzyme, improve cellulosic enzymolysis efficiency further, reduce enzyme cost.
Accompanying drawing explanation
Fig. 1 is the process chart of the present invention;
Wherein, 1-enzymatic vessel, 2 simultaneous saccharification and fermentation tanks, 3-product separation unit.
Detailed description of the invention
By the examples below the inventive method is described further. In the present invention, wt% is mass fraction, and v% is volume fraction.
Embodiment 1
The lignocellulosic material that the embodiment of the present invention uses is Semen Maydis dry straw, wherein cellulose 38.2wt%, and hemicellulose 22.1wt%, lignin 20.2wt%, ash 3.9wt%, being crushed to granular size with pulverizer is 1-5mm. Adopting dilute acid steam explosion to carry out pretreatment, reaction temperature is 190 DEG C, response time 5min, and solid-to-liquid ratio is 1:2, and dilute sulfuric acid concentration is 2.0wt%. Out regulating pH with NaOH afterwards from steam blasting device is 5.0, and wherein dry substance concentration is 32wt%, and in dry, content of cellulose is 40wt%, and the pretreatment dry response rate is 96wt%.
Yeast starter liquid culture medium is 2wt% glucose, 2wt% peptone and 1wt% yeast extract, and 115 DEG C of sterilizing 30min are standby. The preparation of yeast starter liquid is divided into 3 grades of cultivations: the first order utilizes inoculating loop to scrape 1-2 ring bacterium mud from inclined-plane, is linked in the 25mL seed culture medium being placed in 100mL triangular flask, 37 DEG C, 100r/m, shaken cultivation 24h; The second level is cultivated and first order culture fluid is all inoculated in the 500mL seed culture medium in 1L fermentation tank, 37 DEG C, 100r/m, cultivates 24h; The third level is cultivated and second level culture fluid is all inoculated in the 25L seed culture medium in 50L fermentation tank, 37 DEG C, 100r/min, cultivates 24h.
By the flow process shown in Fig. 1, the dischargeable capacity adopting vertical enzymatic vessel is 240L, and the dischargeable capacity of simultaneous saccharification and fermentation tank is 240L.The corn straw good by pretreatment, cellulase (believes Bioisystech Co., Ltd purchased from Novi, model is Ctec2, filter paper enzyme activity 135IU/g) and water be added continuously in enzymatic vessel to carry out pre-enzymolysis in proportion, wherein the addition of pretreatment corn straw is 150kg, cellulase addition 1.152kg is equivalent to 8.1IU/g cellulose after adding), xanthan gum addition 0.0384kg, tap water addition 88.8096kg, in enzymatic hydrolysis system, dry substance concentration is 20wt%, in enzymatic vessel, temperature is 58 DEG C, pH5.0, stir speed (S.S.) 50r/min, pre-enzymolysis time is 24h. then it is pumped into simultaneous saccharification and fermentation tank from enzymatic vessel, is concurrently accessed heatproof yeast starter liquid 24L. nitrogenous source adopts carbamide, addition 0.24kg. in simultaneous saccharification and fermentation tank, temperature is 37 DEG C, and stir speed (S.S.) is 100r/min, and the simultaneous saccharification and fermentation time is 96h.
By liquid chromatographic detection, the concentration of alcohol in final fermentation liquid is 4.65wt%. Being 90% through calculating glucose yield, alcohol getting rate is 92%.
Comparative example 1
Handling process is identical with embodiment 1 with process conditions, is different in that: be added without xanthan gum in enzymatic vessel, and pre-hydrolysis temperature is 50 DEG C.
By liquid chromatographic detection, the concentration of alcohol in final fermentation liquid is 4.24wt%. Being 83% through calculating glucose yield, alcohol getting rate is 91%.
Comparative example 2
Handling process is identical with embodiment 1 with process conditions, is different in that: be added without xanthan gum in enzymatic vessel, and pre-hydrolysis temperature is 58 DEG C.
By liquid chromatographic detection, the concentration of alcohol in final fermentation liquid is 3.62wt%. Being 71% through calculating glucose yield, alcohol getting rate is 91%.
Embodiment 2
Lignocellulose high temperature enzymolysis of the present invention and fermentation producing and ethanol method, including following content:
(1) lignocellulosic material corn straw is carried out dilute acid steam explosion combination pretreatment, it is thus achieved that pretreated feedstock;
(2) pretreated feedstock, cellulase, water and xanthan gum being joined and carry out pre-enzymolysis in enzymatic vessel, the dry substance concentration of controlled enzymatic hydrolysis system is 15wt%, and pre-hydrolysis temperature controls at 54 DEG C; The time of staying of controlled enzymatic hydrolysis feed liquid is 8h; The pH of pre-enzymolysis is 4.5; Xanthan gum addition is 0.08g/L; The addition of described cellulase makes cellulase be 5IU/g cellulose with cellulosic ratio in pretreated feedstock;
(3) after pre-enzymolysis, feed liquid is driven in fermentation tank, adds heatproof saccharomyces cerevisiae and carries out simultaneous saccharification and fermentation, simultaneous saccharification and fermentation refer to pre-enzymolysis after residual cellulose at glucose fermentation producing and ethanol while continuing to the process that is hydrolyzed; Described heatproof saccharomyces cerevisiae is can tolerate the heatproof saccharomyces cerevisiae of 36 DEG C; The inoculum concentration of heatproof alcohol fermentation bacterium seed liquor is 1v%; Being made directly simultaneous saccharification and fermentation after enzymolysis, controlling fermentation temperature is 36 DEG C, and fermentation time is 96h; Adding nitrogenous source in simultaneous saccharification and fermentation system, nitrogenous source is selected from yeast extract, and nitrogenous source addition is the 0.02wt% of system gross mass;
(4) fermentation liquid is driven into rectifier unit separating alcohol.
Embodiment 3
Lignocellulose high temperature enzymolysis of the present invention and fermentation producing and ethanol method, including following content:
(1) lignocellulosic material corn straw is carried out dilute acid steam explosion combination pretreatment, it is thus achieved that pretreated feedstock;
(2) pretreated feedstock, cellulase, water and xanthan gum being joined and carry out pre-enzymolysis in enzymatic vessel, the dry substance concentration of controlled enzymatic hydrolysis system is 25wt%;The time of staying of controlled enzymatic hydrolysis feed liquid is 48h; The pH of pre-enzymolysis is 5.5, and temperature is 62 DEG C; Xanthan gum addition is 0.24g/L; The addition of described cellulase makes cellulase be 25IU/g cellulose with cellulosic ratio in pretreated feedstock;
(3) after pre-enzymolysis, feed liquid is driven in fermentation tank, adds heatproof saccharomyces cerevisiae and carries out simultaneous saccharification and fermentation, simultaneous saccharification and fermentation refer to pre-enzymolysis after residual cellulose at glucose fermentation producing and ethanol while continuing to the process that is hydrolyzed; Described heatproof saccharomyces cerevisiae is can tolerate the heatproof saccharomyces cerevisiae of 42 DEG C; The inoculum concentration of heatproof alcohol fermentation bacterium seed liquor is 5v%; Being made directly simultaneous saccharification and fermentation after enzymolysis, controlling fermentation temperature is 42 DEG C, and fermentation time is 12h; Adding nitrogenous source in simultaneous saccharification and fermentation system, nitrogenous source is selected from peptone, and nitrogenous source addition is the 0.2wt% of system gross mass;
(4) fermentation liquid is driven into rectifier unit separating alcohol.
Embodiment 4
Lignocellulose high temperature enzymolysis of the present invention and fermentation producing and ethanol method, including following content:
(1) lignocellulosic material corn straw is carried out dilute acid steam explosion combination pretreatment, it is thus achieved that locate in advance
Reason raw material;
(2) pretreated feedstock, cellulase, water and xanthan gum being joined and carry out pre-enzymolysis in enzymatic vessel, the dry substance concentration of controlled enzymatic hydrolysis system is 15wt%, and pre-hydrolysis temperature controls at 62 DEG C; The time of staying of controlled enzymatic hydrolysis feed liquid is 24h; The pH of pre-enzymolysis is 5.5, and temperature is 54 DEG C; Xanthan gum addition is 0.15g/L; The addition of described cellulase makes cellulase be 15IU/g cellulose with cellulosic ratio in pretreated feedstock;
(3) after pre-enzymolysis, feed liquid is driven in fermentation tank, adds heatproof saccharomyces cerevisiae and carries out simultaneous saccharification and fermentation, simultaneous saccharification and fermentation refer to pre-enzymolysis after residual cellulose at glucose fermentation producing and ethanol while continuing to the process that is hydrolyzed; Described heatproof saccharomyces cerevisiae is can tolerate the heatproof saccharomyces cerevisiae of 40 DEG C; The inoculum concentration of heatproof alcohol fermentation bacterium seed liquor is 3v%; Being made directly simultaneous saccharification and fermentation after enzymolysis, controlling fermentation temperature is 40 DEG C, and fermentation time is 48h; Adding nitrogenous source, nitrogen source urea in simultaneous saccharification and fermentation system, nitrogenous source addition is the 0.1wt% of system gross mass;
(4) fermentation liquid is driven into rectifier unit separating alcohol.
Embodiment 5
In step (2), the time of staying of controlled enzymatic hydrolysis feed liquid is 12h; The pH of pre-enzymolysis is 4.8; Temperature is 56 DEG C. Other step is with embodiment 1.
Embodiment 6
In step (2), the time of staying of controlled enzymatic hydrolysis feed liquid is 24h; The pH of pre-enzymolysis is 5.2; Temperature is 60 DEG C. Other step is with embodiment 1.
Lignocellulosic material in step of the present invention (1) includes the biomass material of all cellulose, such as straw, wood flour, energy-source plant (such as switchgrass) and waste paper etc., it is preferred to corn straw. Described pretreatment mode can adopt all can improve the physics of lignocellulose enzymolysis performance, chemically and thermally chemical technology, including mechanical activation comminution, radiation, microwave, acid treatment, alkali process, steam explosion pretreatment and solvent pre-treatment, or the combination pretreatment etc. of said method, it is preferred to use dilute acid steam explosion combination pretreatment.
The xanthan gum added in step (2) can improve the ability of cellulase tolerance high temperature, and addition is 0.08-0.24g/L.
In step (2), the time of staying of controlled enzymatic hydrolysis feed liquid is 8-48h, it is preferable that 12-24h.The dry substance concentration of enzymatic hydrolysis system is 15wt%-25wt%; Dry substance concentration of the present invention refers to the percentage ratio of soluble solids and insolubility solid masses sum and system gross mass. Described cellulase adopts pheron or the pheron mixture of all hydrolyzable lignocellulose components, can generate cellulase online in factory, it is possible to adopt commercial goods cellulase, as Novi believes the raw enzyme of enzyme or pool. The addition controlling cellulase makes cellulase be 5-25IU/g cellulose with cellulosic ratio in pretreated feedstock. The pH of pre-enzymolysis is 4.5-5.5, it is preferred to 4.8-5.2; Temperature is 54-62 DEG C, it is preferred to 56-60 DEG C.
Simultaneous saccharification and fermentation in step (3) refer to pre-enzymolysis after residual cellulose at glucose fermentation producing and ethanol while continuing to the process that is hydrolyzed. Described heatproof saccharomyces cerevisiae adopts the bacterial strain that can utilize lignocellulosic material fermentation producing and ethanol at higher temperature being currently known, it is preferred to use can tolerate the heatproof saccharomyces cerevisiae of 36-42 DEG C. The training method adopting this area conventional prepares zymocyte seed liquor, and the inoculum concentration of seed liquor is 1v%-5v%. In the present invention, need not regulating pH after enzymolysis, be made directly simultaneous saccharification and fermentation, control fermentation temperature and be 36-42 DEG C, fermentation time is 12-96h. The nitrogenous source added in simultaneous saccharification and fermentation system is selected from one or more in yeast extract, peptone, Semen Maydis pulp, ammonium sulfate or carbamide etc., it is preferable that carbamide, addition is the 0.02wt%-0.2wt% of system gross mass.
Step (4) adopts this area conventional method that the ethanol in fermentation liquid is easily separated.
Claims (9)
1. the method for a lignocellulose high temperature enzyme hydrolysis and fermentation producing and ethanol, it is characterised in that comprise the steps:
(1) lignocellulosic material is carried out pretreatment, it is thus achieved that pretreated feedstock;
(2) pretreated feedstock, cellulase, water and xanthan gum being joined and carry out pre-enzymolysis in enzymatic vessel, the dry substance concentration of controlled enzymatic hydrolysis system is 15wt%-25wt%, and pre-hydrolysis temperature controls at 54-62 DEG C; The time of staying of controlled enzymatic hydrolysis feed liquid is 8-48h; The pH of pre-enzymolysis is 4.5-5.5;
(3) after pre-enzymolysis, feed liquid is driven in fermentation tank, adds heatproof saccharomyces cerevisiae and carries out simultaneous saccharification and fermentation, and fermentation temperature controls at 36-42 DEG C;
(4) fermentation liquid is driven into rectifier unit separating alcohol.
2. the method for a kind of lignocellulose high temperature enzyme hydrolysis and fermentation producing and ethanol according to claim 1, it is characterised in that in step (1), lignocellulose is preferably corn straw, described pretreatment mode preferably employs dilute acid steam explosion combination pretreatment.
3. the method for a kind of lignocellulose high temperature enzyme hydrolysis and fermentation producing and ethanol according to claim 1, it is characterised in that in step (2), xanthan gum addition is 0.08-0.24g/L.
4. the method for a kind of lignocellulose high temperature enzyme hydrolysis and fermentation producing and ethanol according to claim 1, it is characterised in that in step (2), the time of staying of controlled enzymatic hydrolysis feed liquid is preferably 12-24h; The pH of pre-enzymolysis is preferably 4.8-5.2; Temperature is preferably 56-60 DEG C.
5. the method for a kind of lignocellulose high temperature enzyme hydrolysis and fermentation producing and ethanol according to claim 1, it is characterized in that in step (2), the addition of described cellulase makes cellulase be 5-25IU/g cellulose with cellulosic ratio in pretreated feedstock.
6. the method for a kind of lignocellulose high temperature enzyme hydrolysis and fermentation producing and ethanol according to claim 1, it is characterised in that in step (3), simultaneous saccharification and fermentation refer to pre-enzymolysis after residual cellulose at glucose fermentation producing and ethanol while continuing to the process that is hydrolyzed.
7. the method for a kind of lignocellulose high temperature enzyme hydrolysis and fermentation producing and ethanol according to claim 1, it is characterised in that in step (3), described heatproof saccharomyces cerevisiae preferably can tolerate the heatproof saccharomyces cerevisiae of 36-42 DEG C; The inoculum concentration of heatproof alcohol fermentation bacterium seed liquor is 1v%-5v%; Being made directly simultaneous saccharification and fermentation after enzymolysis, control fermentation temperature and be 36-42 DEG C, fermentation time is 12-96h.
8. the method for a kind of lignocellulose high temperature enzyme hydrolysis and fermentation producing and ethanol according to claim 1, it is characterized in that in step (3), simultaneous saccharification and fermentation system adds nitrogenous source, one or more in yeast extract, peptone, Semen Maydis pulp, ammonium sulfate or carbamide of nitrogenous source, nitrogenous source addition is the 0.02wt%-0.2wt% of system gross mass.
9. the method for a kind of lignocellulose high temperature enzyme hydrolysis and fermentation producing and ethanol according to claim 8, it is characterised in that in step (3), the preferred carbamide of nitrogenous source.
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| CN106967757A (en) * | 2017-03-12 | 2017-07-21 | 广西轻工业科学技术研究院 | A kind of preparation method of cellulosic ethanol |
| CN112159824A (en) * | 2020-09-27 | 2021-01-01 | 兴源环境科技股份有限公司 | Full resource recycling method for livestock manure |
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