CN105660633A - Method for soaking flowers with medium-concentration NAA gel - Google Patents

Abstract

The invention discloses a method for soaking flowers with a medium-concentration NAA gel, and belongs to the technical field of plant biotechnology. The method comprises the steps: firstly, preparing 1 mg/mL NAA mother liquor; diluting the mother liquor to a 100 mg/L medium-concentration NAA working liquid; preparing a medium-concentration NAA gel sealing liquid with the sodium carboxymethyl cellulose mass concentration of 1% and the sodium alginate mass concentration of 8% from the working liquid; and soaking flowers blossomed after 1-2 days in the hormone gel sealing liquid for 2-3 s, and then taking out.

Description

A kind of middle concentration NAA gel be stained with colored method

Technical field

Middle concentration NAA gel be stained with a colored method, belong to plant biotechnology field.

Background technology

Being stained with flower is one of the important channel improving plant percentage of fertile fruit, the plurality of advantages such as the method has that cost is low, instant effect, cycle are short, percentage of fertile fruit height. Dissimilar hormone usually can be used, such as NAA, IBA, NAA, 2,4-D, GA when being stained with colored₃, 6-BA, KT, TDZ, TIBA, ethrel, B₉, PP333, MH etc., the interpolation of above-mentioned hormone can enable plant body or in vitro significantly Accelerate bloom, improve and bear fruit, agriculture production plays important purposes. But, under existence conditions, it is stained with, with hormone working fluid, the loss that flower fair causes moisture or nutritive substance due to long-time high temperature, extremely easily causes failure. Therefore, need a kind of fast and convenient method badly for improving the one-tenth flower of plant, fruit-setting rate etc.

Summary of the invention

A kind of middle concentration NAA gel be stained with colored method, it is characterised in that, described method includes hormone mother liquor, working fluid preparation, hormone gel preparation, be stained with flower, concrete operation steps is as follows:

1. hormone mother liquor: precise weight be the NAA of 0.1g with after the dissolve with ethanol of a little 95%, be settled to 100mL with distilled water, be the NAA mother liquor of 1mg/mL;

2. working fluid preparation: above-mentioned mother liquor is diluted to, according to the ratio of volume ratio 1:10, the middle concentration hormone working fluid that concentration is 100mg/L;

3. hormone gel preparation: accurately take 1g Xylo-Mucine and 8g sodium alginate, the hormone working fluid being 100mg/L by the concentration of 100mL under 50 DEG C of water bath condition dissolves for subsequent use;

It is stained with flower: be soaked in above-mentioned hormone gel confining liquid by flower open after 1-2 days and take out after 2-3s.

It is an advantage of the current invention that from the viewpoint of Bao Shui, short root, lure bud, bloom, bear fruit, taking hormone working fluid as solvent, Xylo-Mucine and sodium alginate are gel carrier, are mixed with containing gelating soln dissimilar, concentration hormone. During use, branch, flower are soaked in slightly above-mentioned solution or above-mentioned solution is dripped and be added in axillalry bud place, film after gel drying, can be formed rapidly, features such as reaching minimizing moisture evaporation, be continuously applied hormone, be easy and simple to handle.

Embodiment:

Below in conjunction with specific embodiment, the present invention is conducted further description.

Embodiment

A kind of middle concentration NAA gel be stained with colored method, it is characterised in that, described method includes hormone mother liquor, working fluid preparation, hormone gel preparation, be stained with flower, concrete operation steps is as follows:

1. hormone mother liquor: precise weight be the NAA of 0.1g with after the dissolve with ethanol of a little 95%, be settled to 100mL with distilled water, be the NAA mother liquor of 1mg/mL;

2. working fluid preparation: above-mentioned mother liquor is diluted to, according to the ratio of volume ratio 1:10, the middle concentration hormone working fluid that concentration is 100mg/L;

3. hormone gel preparation: accurately take 1g Xylo-Mucine and 8g sodium alginate, the hormone working fluid being 100mg/L by the concentration of 100mL under 50 DEG C of water bath condition dissolves for subsequent use;

It is stained with flower: be soaked in above-mentioned hormone gel confining liquid by capsicum flower open after 1-2 days and take out after 2-3s.

Claims (1)

1. in one kind concentration NAA gel be stained with colored method, it is characterised in that, described method includes hormone mother liquor, working fluid preparation, the preparation of hormone gel, is stained with flower, concrete operation steps is as follows:

1. hormone mother liquor: precise weight be the NAA of 0.1g with after the dissolve with ethanol of a little 95%, be settled to 100mL with distilled water, be the NAA mother liquor of 1mg/mL;

2. working fluid preparation: above-mentioned mother liquor is diluted to, according to the ratio of volume ratio 1:10, the middle concentration hormone working fluid that concentration is 100mg/L;

3. hormone gel preparation: accurately take 1g Xylo-Mucine and 8g sodium alginate, the hormone working fluid being 100mg/L by the concentration of 100mL under 50 DEG C of water bath condition dissolves for subsequent use;

It is stained with flower: be soaked in above-mentioned hormone gel confining liquid by flower open after 1-2 days and take out after 2-3s.