CN105651656B - The device and its working method of grain shape are analyzed based on laser hologram imaging method - Google Patents
The device and its working method of grain shape are analyzed based on laser hologram imaging method Download PDFInfo
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Abstract
The present invention relates to grading analysis technical fields, disclose a kind of device and its working method for analyzing grain shape based on laser hologram imaging method.Described device includes laser, microlens, diaphragm, transparent observation part and imaging sensor, wherein is provided with fluid manhole in the transparent observation part.Using laser hologram imaging method directly to the particle in fluid to be measured into the holographic imaging information passed through interference effect and generated, then data inversion processing is carried out to holographic imaging information, it can be obtained the aerial image information of reflection grain shape, therefore without using filter membrane, avoidable sample is contaminated, and then measurement error and consumptive material expense can be reduced, simplify the operating process of equipment.In addition, described device also has the advantages that real-time, particle resolution ratio is high and detection object is wide, it is convenient for practical application and popularization.
Description
Technical field
The present invention relates to grading analysis technical fields, and in particular, to one kind analyzing particle based on laser hologram imaging method
The device and its working method of shape.
Background technology
Currently, in order to cope with, solve industrial production and people occupies problem of environmental pollution caused by life style, especially endanger
The serious haze problem of evil is needed to carry out continuous, accurate and extensive monitoring to the particulate matter characteristic in air, such as is carried out
The measurement work of PM2.5 and PM10.The existing measurement method for particulate in air matter characteristic mainly has gravimetric method, concussion
Sedimentation balance method and Beta ray methods etc..Wherein, the principle of the gravimetric method is respectively by the sampler of certain Cutting feature, with perseverance
Speed extracts determined volume air, so that PM2.5 and PM10 in surrounding air is trapped on the filter membrane of known quality, according to sampling
The of poor quality and sampling volume of front and back filter membrane, calculates the concentration of PM2.5 and PM10；The principle of the concussion sedimentation balance method is in matter
Using an oscillation hollow conical pipe in quantity sensor, and in its vibration end replaceable filter membrane is installed (frequency of oscillation depends on
Conical pipe feature and its quality), when sampling air flow is by filter membrane, particulate matter therein is deposited on filter membrane, and the quality of filter membrane becomes
Changing leads to the variation of frequency of oscillation, the quality for being deposited on particulate matter on filter membrane is calculated by frequency of oscillation variation, further according to stream
Amount, site environment temperature and air pressure calculate the mass concentration of the period particulate matter mark；The principle of the Beta ray methods is
Using the principle of Beta ray attenuations, surrounding air sucks sampling pipe by sampling pump, goes out through filter membrane heel row, particulate matter is deposited in
On filter membrane, when β rays are by the filter membrane that deposits particulate matter, the energy attenuation of Beta rays passes through the measurement to attenuation
The concentration of particulate matter can be calculated.
But these types of mode is required for being filtered screening to the particle in air in advance using filter membrane, therefore carry out
It is to damage detection, is easy that sample is made to be contaminated, such as influenced by air humidity, thus there are measurement errors big, consumptive material
Costly, instrument needs often calibration and problem complicated for operation.
Invention content
That there are measurement errors is big, consumptive material is costly, instrument needs often calibration for above-mentioned existing several method at present and
Problem complicated for operation, the present invention provides a kind of device and its work sides analyzing grain shape based on laser hologram imaging method
Method, can be using laser hologram imaging method directly to the particle in fluid to be measured into the holographic imaging passed through interference effect and generated
Then information carries out data inversion processing to holographic imaging information, you can the aerial image information of reflection grain shape is obtained, by
This is contaminated without using filter membrane, avoidable sample, and then can reduce measurement error and consumptive material expense, simplifies the behaviour of equipment
Make process.In addition, described device also has the advantages that real-time, particle resolution ratio is high and detection object is wide, convenient for actually answering
With and promote.
On the one hand the technical solution adopted by the present invention provides and a kind of analyzing grain shape based on laser hologram imaging method
Device, including laser, microlens, diaphragm, transparent observation part and imaging sensor, wherein opened in the transparent observation part
There is fluid manhole；The laser, the microlens, the diaphragm, the transparent observation part and described image sensing
Device sequentially coaxially heart line is arranged, and the optical focus of the microlens is located in the axle center hole of the diaphragm, the diaphragm
The first end face of end face and the transparent observation part offsets, the second end face and described image sensor of the transparent observation part
Sensitive surface offsets.
Specifically, the laser is pulse laser of the optical maser wavelength between 355~635 nanometers；Alternatively, described
Laser is modulated continuous wave laser of the optical maser wavelength between 355~635 nanometers.
Specifically, being embedded with anti-reflection coating between the end face of the diaphragm and the first end face of the transparent observation part；With/
Or, being embedded with anti-reflection coating between the second end face and the sensitive surface of described image sensor of the transparent observation part.Further
Specifically, the anti-reflection coating is optical adhesive layer or immersion oil layer.
Specifically, the distance of the diaphragm to the fluid manhole is set as L1, the fluid manhole is to described
The distance of imaging sensor is set as L2, then the two segment distances L1And L2Meet following relationship：
In formula, λ0For optical maser wavelength, η is the refractive index of the transparent observation part, and d is the pixel ruler of described image sensor
Very little size, D are the actual induction area size of described image sensor.
Specifically, transparent material of the transparent observation part by refractive index more than 1.5 is made.
Specifically, a diameter of 1.22 λ of the axle center hole of the diaphragm0, in formula, λ0For optical maser wavelength.
Specifically, described image sensor is ccd sensor or cmos sensor.
On the other hand the technical solution adopted by the present invention provides aforementioned based on laser hologram imaging method analysis grain shape
Device working method, include the following steps：S101. the fluid manhole into transparent observation part imports fluid to be measured；
S102. start laser, emit pulse type laser；S103. so that the laser is passed through microlens, and focus on the axle center of diaphragm
Kong Zhong, then diffraction formed point source spherical wave；S104. the point source spherical wave passes through transparent observation part, and across the stream
When body manhole particle diffracted wave is formed with the Interaction between particles in the fluid to be measured；S105. the point source spherical wave
It interferes, and is incident upon in the sensitive surface of imaging sensor with the particle diffracted wave, generate holographic imaging information；S106.
The computer receives the holographic imaging information from described image sensor, handles to obtain reflection by data inversion
The aerial image information of particle shape shape.
Specifically, the fluid to be measured is liquid or gas.
To sum up, using device and its work side provided by the present invention for analyzing grain shape based on laser hologram imaging method
Method has the advantages that：(1) laser hologram imaging method can be utilized directly to interfere into passing through the particle in fluid to be measured
Then the holographic imaging information of effect and generation carries out data inversion processing to holographic imaging information, you can obtain reflection particle
The aerial image information of shape, therefore without using filter membrane, avoidable sample to be contaminated, and then can reduce measurement error and
Consumptive material expense simplifies the operating process of equipment；(2) it is convenient for carrying out induction image forming information online data using computer system
Inversion procedure is conveniently made instantly available measurement result, real-time；(3) 0.2 micron of even higher resolution ratio can be reached, it can
With solve the problems, such as it is existing limited by optical limit based on microscopy and its resolution ratio, airborne particulate substance is measured
It is of great significance；(4) described device not only can carry out image checking to the particle in gas, can also be in liquid
Grain carries out image checking, and object to be measured is wider, is convenient for practical application and popularization.
Description of the drawings
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technology description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with
Obtain other attached drawings according to these attached drawings.
Fig. 1 is the structural schematic diagram of the device provided by the invention that grain shape is analyzed based on laser hologram imaging method.
Fig. 2 is the workflow schematic diagram of the device provided by the invention.
In above-mentioned attached drawing：1, laser 2, microlens 3, diaphragm 4, transparent observation part 401, fluid manhole
5, imaging sensor 6, computer 7, anti-reflection coating.
Specific implementation mode
Hereinafter reference will be made to the drawings, is described in detail by way of example provided by the invention based on laser hologram imaging method
Analyze the device and its working method of grain shape.It should be noted that the explanation for these way of example is used for
Help understands the present invention, but does not constitute limitation of the invention.
Various technologies described herein can be used for but be not limited to grading analysis technical field, can be also used for other classes
Like field.
The terms "and/or", only a kind of incidence relation of description affiliated partner, indicates that there may be three kinds of passes
System, for example, A and/or B, can indicate：Individualism A, individualism B exist simultaneously tri- kinds of situations of A and B, the terms
"/and " it is another affiliated partner relationship of description, indicate may exist two kinds of relationships, for example, A/ and B, can indicate：Individually deposit
In A, two kinds of situations of individualism A and B, in addition, character "/" herein, it is a kind of "or" pass to typically represent forward-backward correlation object
System.
Embodiment one
Fig. 1 shows the structural representation of the device provided by the invention that grain shape is analyzed based on laser hologram imaging method
Figure, Fig. 2 shows the workflow schematic diagrams of the device provided by the invention.It is provided in this embodiment described based on laser hologram
Imaging method analyzes the device of grain shape, including laser 1, microlens 2, diaphragm 3, transparent observation part 4 and imaging sensor
5, wherein be provided with fluid manhole 401 in the transparent observation part 4；The laser 1, the microlens 2, the light
Door screen 3, the transparent observation part 4 and described image sensor 5 sequentially coaxially heart line are arranged, and the optics of the microlens 2 is burnt
In the axle center hole of the diaphragm 3, the end face of the diaphragm 3 and the first end face of the transparent observation part 4 offset point, described
The sensitive surface of the second end face and described image sensor 5 of transparent observation part 4 offsets.
Further include the computer 6 for communicating to connect described image sensor 5 as shown in Figure 1, in the structure of described device.
The laser 1 is used to emit pulse type laser to 5 Directional discontinuity of described image sensor along axial line, and the pulsed swashs
The frequency requirement of light and the picture-taken frequency of described image sensor 5 match, and the pulse width of the pulse type laser is wanted
The Minimum Exposure Time more than described image sensor 5 is sought, to which preferable induction can be generated so as to described image sensor 5
Image；The microlens 2 are used to carry out further focusing light effect to laser, to spread out at the axle center hole of the diaphragm 3
It penetrates to form point source spherical wave；The transparent observation part 4 makes point source spherical wave across the stream for providing particle diffraction place
When body manhole, particle diffracted wave can be formed with the Interaction between particles in the fluid to be measured, while so as to generation
Particle diffracted wave is interfered with point source spherical wave, and is projected in the sensitive surface of described image sensor 5, and holographic induction is formed
Image, the fluid manhole 401 therein wait for side liquid for importing and exporting；Described image sensor 5 is for carrying out
Light wave induction image forming, and obtain holographic imaging information corresponding with holographic sensed image by opto-electronic conversion；The computer 6 is used
In utilizing existing data inversion treatment technology, mathematics is carried out to the holographic imaging information from described image sensor 5
Inversion procedure obtains the aerial image information of reflection grain shape, such as the three-dimensional image information etc. of particle.It is possible thereby to utilize
Laser hologram imaging method is then right directly to the particle in fluid to be measured into the holographic imaging information passed through interference effect and generated
Holographic imaging information carries out data inversion processing, you can obtains the aerial image information of reflection grain shape, is not necessarily in application
Using filter membrane, avoidable sample is contaminated, and then can reduce measurement error and consumptive material expense, simplifies the operation of equipment
Journey, described device also has the advantages that real-time wide with detection object in addition, is convenient for practical application and popularization.
Specifically, the laser 1 can be, but not limited to as optical maser wavelength between 355~635 nanometers (such as wavelength
For 355 nanometers of laser, wavelength be 375 nanometers laser, the laser that wavelength is 405 nanometers, the laser that wavelength is 473 nanometers,
The laser etc. that laser, the laser that wavelength is 532 nanometers and the wavelength that wavelength is 488 nanometers are 635 nanometers) pulse laser；
Alternatively, the laser 1 can be, but not limited to as optical maser wavelength between 355~635 nanometers, (such as wavelength is 355 nanometers
Laser, the laser that wavelength is 375 nanometers, the laser that wavelength is 405 nanometers, the laser that wavelength is 473 nanometers, wavelength 488
Nanometer laser, the laser that wavelength is 532 nanometers and wavelength be 635 nanometers laser etc.) modulated continuous wave laser,
In, the modulation system of the modulated continuous wave laser can be, but not limited to as acousto-optic modulation mode (i.e. in continuous wave laser
Transmitting terminal configure an acousto-optic modulator) or Electro-optical Modulation mode (i.e. the transmitting terminal of continuous wave laser configuration one electric light tune
Device processed).According to Rayleigh criterion in optical theory (the discernmible shortest distance is approximately equal to the radius of Airy between two picture points),
Understand the greatest optical resolution ratio σ of described deviceMaxFor：
In formula, λ0For optical maser wavelength, η be the transparent observation part 4 refractive index (refer to the absolute index of refraction of relative vacuum,
It is identical in full), NA is numerical aperture.Therefore the greatest optical resolution ratio σ of described deviceMaxNumerical values recited directly and laser wave
Long λ0It is directly proportional：I.e. optical maser wavelength is shorter, σMaxNumerical value it is smaller, the resolution ratio of reflection is higher.From there through to the laser
1 carries out the restriction of the laser, it can be ensured that described device has higher optical resolution.
Specifically, being embedded with anti-reflection coating 7 between the end face of the diaphragm 3 and the first end face of the transparent observation part 4；
And/or it is embedded with anti-reflection coating 7 between the second end face and the sensitive surface of described image sensor 5 of the transparent observation part 4.
As shown in Figure 1, by the way that the anti-reflection coating 7 is arranged, light wave can be alleviated and produced at the corresponding end surface of the transparent observation part 4
Raw reflection is lost from that can reduce light-wave energy, inductive effects of the guarantee light wave in described image sensor 5.It is further specific
, the anti-reflection coating 7 can be but not limited to as optical adhesive layer or immersion oil layer.As an optimization, in the present embodiment, institute
It states anti-reflection coating 7 and uses immersion oil layer, the transparent observation part 4 can be also convenient on the basis of ensuring anti-reflection effect
It replaces and cleans.
Specifically, the distance of the diaphragm 3 to the fluid manhole 401 is set as L1, the fluid manhole 401
Distance to described image sensor 5 is set as L2, then the two segment distances L1And L2Meet following relationship：
In formula, λ0For optical maser wavelength, η is the refractive index of the transparent observation part 4, and d is the pixel of described image sensor 5
Size, D are the actual induction area size of described image sensor 5.As shown in Figure 1, in the structure of described device,
The actual induction area of described image sensor 5 is located in the sensitive surface of described image inductor 5, can be, but not limited to be described
The border circular areas or square area that the second end face of transparent observation part 4 and the sensitive surface of described image sensor 5 offset, when this
When region is round, corresponding actual induction area size D is round diameter, corresponding to have when the region is square
Effect induction zone size D is square the length of side.(such as it is when FFT is handled for the ease of subsequently carrying out data inversion processing
Convenient for determining areas imaging), the actual induction area is preferably square, while requiring single-row in the actual induction area
Or single row of pixels number is 2nA (n is natural number), such as when the actual induction area is 512 × 512 pixel size, it is corresponding
Actual induction area size D=512d；It is corresponding or when the actual induction area is 1024 × 1024 pixel size
Actual induction area size D=1024d；It is corresponding or when the actual induction area is 2048 × 2048 pixel size
Actual induction area size D=2048d.
Simultaneously as shown in Figure 1, in the structure of the transparent observation part 4, since its correspondence can be obtained according to optical theory
Numerical aperture NA' be：
, then it is described it is transparent observation part 4 greatest optical resolution ratio σ 'MaxFor：
, then further, the maximum pixel resolution ratio on described image bed sensor 5 can be obtainedFor：
, while in order to enable the minimum sensing unit on described image inductor 5 and the maximum pixel resolution ratioMatch, needs to meet following relationship：
, from there through the limited number of time derivation of equation, the two segment distances L can be obtained1And L2Following relationship limit：
, the transparent observation part 4 is configured according to relationship restriction, the whole device can be made to obtain one
Rational Optical System Design, and obtain higher pixel resolution on described image bed sensor 5.
The available maximum pixel resolution ratio on described image bed sensor 5 is illustrated with an example below：It is described to swash
Light device 1 is the pulse laser that launch wavelength is 405 nanometer lasers, and the microlens 2 are that amplification factor is 40 times and numerical value
The camera lens that aperture is 0.75；The diaphragm 3 to the fluid manhole 401 distance L1=0.6mm, the fluid access hole
Road 401 to described image sensor 5 distance L2=9.3mm, the cell wide of the fluid manhole 401 be 0.1mm (
Calculate maximum pixel resolution ratio when ignore), it is described it is transparent observation part 4 refractive index be 1.74 (the transparent observation part 4 can with but
It is not limited to be made of 1.74 resin material of refractive index), the Pixel Dimensions d=3.45um of described image sensor 5, sensitive surface
On actual induction area size D=2048d=7.0656mm, then meeting the two segment distances L1And L2The relationship
It is available maximum as number resolution ratio is on the basis of restriction(at this time), Ke Yida
To 0.2 micron of rank.If using the shorter laser emitter of optical maser wavelength, it can also further make maximum as number resolution ratioNumerical value it is lower.
Specifically, the transparent material that the transparent observation part 4 can be, but not limited to by refractive index more than 1.5 is made.According to
The greatest optical resolution ratio σ of described device above-mentionedMax：
It is found that the greatest optical resolution ratio σ of described deviceMaxNumerical values recited be directly inversely proportional with refractive index η：Reflect
Rate η is higher, σMaxNumerical value it is smaller, the resolution ratio of reflection is higher.The material is carried out from there through to the transparent observation part 4
Restriction, it can be ensured that described device have higher optical resolution.
Specifically, the axle center bore dia of the diaphragm 3 can be, but not limited to as 1.22 λ0, in formula, λ0For optical maser wavelength.Institute
Although the axle center bore dia for stating diaphragm 3 might be less that 1.22 λ0, but laser light energy can be caused to lose in this way, therefore in this reality
It applies in example, the axle center bore dia of the diaphragm 3 is preferably 1.22 λ0。
Specifically, described image sensor 5 can be, but not limited to as CCD (Charge-coupledDevice, Charged Couple
Element) (Complementary Metal-Oxide Semiconductor, Complimentary Metal-Oxide are partly led by sensor or CMOS
Body) sensor.As an optimization, in the present embodiment, described image sensor 5 uses cmos sensor, and high quality can be obtained
Hologram image and holographic imaging information on the basis of, so that described device is had the advantages that small power consumption and price are low etc..
As shown in Fig. 2, it is aforementioned based on laser hologram imaging method analyze grain shape device working method, can with but not
It is limited to include the following steps：S101. the fluid manhole into transparent observation part imports fluid to be measured；S102. start laser
Device emits pulse type laser；S103. so that the laser is passed through microlens, and focus in the axle center hole of diaphragm, then diffraction
Form point source spherical wave；S104. the point source spherical wave pass through transparent observation part, and when across the fluid manhole with
Interaction between particles in the fluid to be measured form particle diffracted wave；S105. the point source spherical wave and the particle diffraction
Wave interferes, and is incident upon in the sensitive surface of imaging sensor, generates holographic imaging information；S106. by the holographic imaging
Information is sent to computer, carries out data inversion processing, finally obtains the aerial image information of reflection grain shape.
In the step S101, the fluid to be measured can be, but not limited to as liquid or gas, for example, ambient air or
Person's tap water.In the step S106, the mode of the data inversion processing (also referred to as image reproducing processing) is existing number
According to treatment technology, can be, but not limited to as following processing mode：
Ir=iFFT (FFT (Ih)·FFT(Ipsf))
In formula, FFT () is Fourier transform function, and iFFT () is inverse-Fourier transform function, IrBelieve for aerial image
Breath, IhFor holographic imaging information, IpsfFor point source image information.In data inversion processing procedure, due to the fluid access hole
Road 401 has certain width (such as 0.1mm), and the size of particle is in contrast much smaller (about at um grades), therefore
It first needs to be focused automatically, inverting locks lateral position of the particle in the fluid manhole 401, then inverting obtains instead
Reflect the aerial image information of grain shape.The entity space image of particle is shown and is divided finally by the computer
Analysis, such as gray analysis or particle detections analysis, so that it may with observed results such as the size or the shapes that obtain particle.
The device and its working method that grain shape is analyzed based on laser hologram imaging method provided in this embodiment, tool
There is following technique effect：(1) laser hologram imaging method can be utilized directly to the particle in fluid to be measured into interference effect of passing through
And the holographic imaging information generated, data inversion processing then is carried out to holographic imaging information, you can obtain reflection grain shape
Aerial image information, therefore without using filter membrane, avoidable sample to be contaminated, and then measurement error and consumptive material can be reduced
Expense simplifies the operating process of equipment；(2) it is convenient for carrying out induction image forming information online data inversion using computer system
Processing, is conveniently made instantly available measurement result, real-time；(3) 0.2 micron of even higher resolution ratio can be reached, can be solved
Certainly existing the problem of being limited by optical limit based on microscopy and its resolution ratio, have for airborne particulate substance measurement
Significance；(4) described device not only can to the particle in gas carry out image checking, can also to the particle in liquid into
Row image checking, object to be measured is wider, is convenient for practical application and popularization.
As described above, the present invention can be realized preferably.For a person skilled in the art, religion according to the present invention
It leads, designs various forms of devices based on laser hologram imaging method analysis grain shape and its working method does not need to create
The labour for the property made.These embodiments are changed without departing from the principles and spirit of the present invention, are changed, are replaced,
It integrates and modification is still fallen in protection scope of the present invention.
Claims (9)
1. a kind of working method of device that analyzing grain shape based on laser hologram imaging method, which is characterized in that described to be based on
The device that laser hologram imaging method analyzes grain shape includes laser (1), microlens (2), diaphragm (3), transparent observation part
(4) and imaging sensor (5), wherein be provided with fluid manhole (401), the laser in the transparent observation part (4)
(1), the microlens (2), the diaphragm (3), the transparent observation part (4) and described image sensor (5) be sequentially coaxially
Heart line is arranged, and the optical focus of the microlens (2) is located in the axle center hole of the diaphragm (3), the end of the diaphragm (3)
Face and the first end face of the transparent observation part (4) offset, and the second end face of the transparent observation part (4) is sensed with described image
The sensitive surface of device (5) offsets；
The working method of the device that grain shape is analyzed based on laser hologram imaging method is included the following steps：
S101. the fluid manhole into transparent observation part imports fluid to be measured；
S102. start laser, emit pulse type laser；
S103. so that the laser is passed through microlens, and focus in the axle center hole of diaphragm, then diffraction forms point source spherical surface
Wave；
S104. the point source spherical wave passes through transparent observation part, and waits for flow measurement with described when across the fluid manhole
Interaction between particles in body form particle diffracted wave；
S105. the point source spherical wave and the particle diffracted wave interfere, and are incident upon in the sensitive surface of imaging sensor,
Generate holographic imaging information；
S106. the holographic imaging information is sent to computer, carries out data inversion processing, finally obtains reflection grain shape
Aerial image information.
2. the working method of the device of grain shape, feature are analyzed based on laser hologram imaging method as described in claim 1
It is, the laser (1) is pulse laser of the optical maser wavelength between 355~635 nanometers；
Alternatively, the laser (1) is modulated continuous wave laser of the optical maser wavelength between 355~635 nanometers.
3. the working method of the device of grain shape, feature are analyzed based on laser hologram imaging method as described in claim 1
It is, anti-reflection coating (7) is embedded between the end face of the diaphragm (3) and the first end face of the transparent observation part (4)；
And/or it is embedded with anti-reflective between the second end face and the sensitive surface of described image sensor (5) of the transparent observation part (4)
Penetrate coating (7).
4. the working method of the device of grain shape, feature are analyzed based on laser hologram imaging method as claimed in claim 3
It is, the anti-reflection coating (7) is optical adhesive layer or immersion oil layer.
5. the working method of the device of grain shape, feature are analyzed based on laser hologram imaging method as described in claim 1
It is, the distance of the diaphragm (3) to the fluid manhole (401) is set as L1, the fluid manhole (401) to institute
The distance for stating imaging sensor (5) is set as L2, then the two segment distances L1And L2Meet following relationship：
In formula, λ0For optical maser wavelength, η is the refractive index of the transparent observation part (4), and d is the pixel of described image sensor (5)
Size, D are the actual induction area size of described image sensor (5).
6. the working method of the device of grain shape, feature are analyzed based on laser hologram imaging method as described in claim 1
It is, transparent material of the transparent observation part (4) by refractive index more than 1.5 is made.
7. the working method of the device of grain shape, feature are analyzed based on laser hologram imaging method as described in claim 1
It is, a diameter of 1.22 λ of axle center hole of the diaphragm (3)0, in formula, λ0For optical maser wavelength.
8. the working method of the device of grain shape, feature are analyzed based on laser hologram imaging method as described in claim 1
It is, described image sensor (5) is ccd sensor or cmos sensor.
9. the working method of the device of grain shape, feature are analyzed based on laser hologram imaging method as described in claim 1
It is, the fluid to be measured is liquid or gas.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN113495043B (en) * | 2020-03-18 | 2023-11-10 | 江苏长电科技股份有限公司 | Micro-scale diffraction image detection device |
| CN116337699A (en) * | 2021-12-24 | 2023-06-27 | 晋城三赢精密电子有限公司 | Particulate optical detection system and method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1564086A (en) * | 2004-03-29 | 2005-01-12 | 深圳市泛彩溢实业有限公司 | Mfg. method and appts. for digital speckle hologram |
| CN101943663A (en) * | 2010-07-09 | 2011-01-12 | 董珂 | Measuring analytical system and measuring analytical method for distinguishing diffraction image of particles automatically |
| KR20110127504A (en) * | 2010-05-19 | 2011-11-25 | (주)에이치시티 | Paticle measurement apparatus |
| CN205607811U (en) * | 2016-04-01 | 2016-09-28 | 黄恺 | Device based on laser holography formation of image method analysis grain shape |
-
2016
- 2016-04-01 CN CN201610207512.4A patent/CN105651656B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1564086A (en) * | 2004-03-29 | 2005-01-12 | 深圳市泛彩溢实业有限公司 | Mfg. method and appts. for digital speckle hologram |
| KR20110127504A (en) * | 2010-05-19 | 2011-11-25 | (주)에이치시티 | Paticle measurement apparatus |
| CN101943663A (en) * | 2010-07-09 | 2011-01-12 | 董珂 | Measuring analytical system and measuring analytical method for distinguishing diffraction image of particles automatically |
| CN205607811U (en) * | 2016-04-01 | 2016-09-28 | 黄恺 | Device based on laser holography formation of image method analysis grain shape |
Non-Patent Citations (2)
| Title |
|---|
| 激光全息技术在管道测量中的应用与研究;刘乾;《中国优秀硕士学位论文全文数据库 工程科技Ⅱ辑》;20140115(第1期);正文第1.3节和第3章 * |
| 粒度测试现状;胡荣泽;《粉末冶金技术》;19841231(第4期);第40-44页 * |
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