CN105646689A - Polypeptide and detection device and detection kit containing polypeptide - Google Patents
Polypeptide and detection device and detection kit containing polypeptide Download PDFInfo
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- CN105646689A CN105646689A CN201410628804.6A CN201410628804A CN105646689A CN 105646689 A CN105646689 A CN 105646689A CN 201410628804 A CN201410628804 A CN 201410628804A CN 105646689 A CN105646689 A CN 105646689A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
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Abstract
The invention relates to a polypeptide and a detection device and detection kit containing the polypeptide. The polypeptide disclosed by the invention is made up of an amino acid sequence represented by SEQ ID NO: 1 shown in the description. The polypeptide and the detection device and detection kit containing the polypeptide, disclosed by the invention, are useful in malaria diagnosis.
Description
Technical field
The present invention relates generally to polypeptide, comprises the detection means of this polypeptide and detection kit, belongs to biological technical field.
Background technology
Malaria is a kind of acute infectious disease, is mosquito matchmaker's Protozool diseases in the world that mankind's harm is serious at present, and pathogenic agent is plasmodium, and symptom is heating of periodically feeling cold. Owing to the life cycle of plasmodium is complicated, antigen has the features such as phasic specificity, and the control of malaria becomes international one of difficult problem. Wherein, pernicious malaria infects 3-5 hundred million people every year, captures the life of millions of people. In November, 2011, World Health Organization's recent statistics has 2.16 hundred million malaria cases to occur, and mortality ratio is up to 655,000 people. Plasmodium is mainly distributed in the earth torrid zone, subtropics various countries, and namely the area such as Africa, South East Asia, South America, about has 2,000,000,000 populations still to live in epidemic-stricken area, die from the children about 1,000,000 of malaria every year. China in south China, the certain areas, particularly Yunnan in Central China and Hainan Province be district occurred frequently. Therefore, malaria also seriously hinders the Economic development of developing country.
Due to the singularity of malaria treatment medicine, accurately diagnosis fast can not only Timeliness coverage and treat malaria patients, greatly reduce case fatality rate, also by getting rid of malaria in early days, save in time the life having infected other communicable disease patient. With this simultaneously, accurately early diagnosis fast is also the necessary means to the real-time prevailing disease monitor and forecast of malaria. At present, the classical way of diagnosis malaria is still blood smear microscopy, and the method is very high to the technical ability of proofer and survey requirement, cannot meet most needs living in remote districts patient in the developing countries such as Africa and South America far away.
Summary of the invention
It is an object of the invention to provide the purposes in the disease (such as malaria) that a kind of useful polypeptide of diagnosis to malaria, the antibody of this polypeptide anti-, the detection means comprising this polypeptide, the detection kit comprising this polypeptide or this detection means and this polypeptide cause detection plasmodium, this polypeptide purposes in the test kit or detection means of the disease (such as malaria) caused for the preparation of detection plasmodium.
That is, the present invention comprises following technical proposals:
1. the polypeptide being made up of the aminoacid sequence shown in SEQIDNO:1.
2. a detection means, comprising:
Solid carrier, and
The polypeptide described in item 1 being connected on this solid carrier.
3. detection means according to item 2, wherein, described solid carrier is SJ modified silica-gel.
4. a detection kit, it comprises the polypeptide described in item 1 or the detection means described in item 2 or 3.
5. the antibody of polypeptide described in anti-item 1.
6. purposes in the test kit or detection means of the disease caused for the preparation of detection plasmodium of polypeptide described in 1.
7. purposes described in 6, wherein, described disease is malaria.
8. purposes described in 6 or 7, wherein, described plasmodium is subtertian malaria.
9. encode the nucleic acid of the polypeptide described in item 1.
10. comprise the expression vector of the nucleic acid described in item 9.
11. have imported the host cell of the expression vector described in item 10.
The polypeptide of the present invention is applied to the diagnosis of the disease (such as malaria) that plasmodium causes, it is possible to obtain gratifying effect.
Accompanying drawing explanation
Polypeptide is fixed on the schematic diagram on SJ modified silica-gel surface by Fig. 1 by chemistry covalency.
The schematic diagram that the making processes of SJ modified silica-gel (iPDMS film) is described by Fig. 2.
The figure that polypeptide microarray chemistry fixation procedure is described by Fig. 3.
Fig. 4 illustrates the schematic diagram of polypeptide microarray spot sample mode.
Fig. 5 shows the photo to the result that healthy normal people or non-malaria disease human serum detect.
Fig. 6 shows the photo to the result that malaria disease human serum detects.
Embodiment
The polypeptide of the present invention
The polypeptide of the present invention is 30 peptides. 30 peptides of the present invention are made up of the aminoacid sequence shown in SEQIDNO:1, that is: KDVIEQEIVSEEVNEKDTKNNDKKIGKRVK. As shown in the Examples, its reaction that serum of malaria patient is positive, and reaction that healthy normal people or non-malaria disease human serum are negative. Therefore, the diagnostic tool of the disease (such as malaria) that this 30 peptide causes as plasmodium (such as subtertian malaria) is useful.
The polypeptide of the present invention is when having commercially available, it is possible to use commercially available product, in addition, it is also possible to the suitable employing known method such as (1) chemical synthesis process or (2) enzyme reaction synthetic method obtains, and wherein chemosynthesis is more easy. In the polypeptide situation of chemosynthesis the present invention, carry out with the use of the synthesis of peptide symthesis instrument or this polypeptide semi-synthetic. As chemical synthesis process, it is possible to list such as peptide solid-phase synthesis etc. The peptide of synthesis can adopt conventional means such as ion-exchange chromatography, reversed phase high efficiency liquid color spectrum, affinity chromatography etc. to carry out purifying like this. Such peptide solid phase synthesis process with and subsequent peptide purification be all well-known in the art.
In addition, when by the polypeptide of enzyme reaction production the present invention, it is possible to adopt such as method described in No. WO2004/011653, International Publication brochure. Namely; can produce like this: the amino acid (amino acid of such as carboxy protective) that the amino acid esterified for the C-terminal of the amino acid of a side or dipeptides or amidation obtained or dipeptides and amino acid are in unbound state reacts under the existence of peptide synthetase, the dipeptides of generation or tripeptides. As peptide synthetase, it is possible to list: bacterial disposing thing or this microbe-derived peptide synthetase with the culture of the microorganism of the ability generating peptide, the microbial cells being separated by this culture or this microorganism.
And, except above-mentioned enzyme method, chemical synthesis process, in some situation, the polypeptide of the present invention may be also natural existence (but not being separated). In naturally occurring situation, it is also possible to be separated.
The nucleic acid of the present invention, expression vector host cell, and the antibody of the polypeptide of anti-the present invention
The present invention also relates to the coding nucleic acid (nucleic acid of the present invention) of this polypeptide, the expression vector (expression vector of the present invention) comprising this nucleic acid, the host cell (host cell of the present invention) that imported this expression vector, and they preferably can be used for the polypeptide of production the present invention.The nucleic acid of the present invention, expression vector, host cell can adopt the method for well known to a person skilled in the art to prepare. The present invention also relates to the antibody of the polypeptide of anti-the present invention, and it can be used for detecting the antibody of the present invention. The antibody of the present invention can adopt the method for well known to a person skilled in the art to prepare.
The detection means of the present invention
The present invention also relates to a kind of detection means (detection means of the present invention), it polypeptide of the present invention comprising solid carrier and being connected on this solid carrier.
In the present invention, solid carrier is not particularly limited, as long as the carrier of solid or insoluble material (being such as the material can being separated from reaction mixture by filtration, precipitation, magnetic resolution etc.).
The material forming solid carrier includes but not limited to: silica gel (polydimethylsiloxane, PDMS), Mierocrystalline cellulose, teflonTM, Nitrocellulose, agarose, dextran, chitosan, polystyrene, polyacrylamide, polyester, polycarbonate, polymeric amide, polypropylene, nylon, poly(vinylidene fluoride), latex, silicon-dioxide, glass, glass fibre, gold, platinum, silver, copper, iron, stainless steel, ferrite, silicon wafer, polyethylene, polymine, poly(lactic acid), resin, polyose, albumen (albumin etc.), carbon or their combination etc.
The shape of solid carrier comprises but is not limited to: pearl, magnetic bead, film, microcapillary, filter membrane, plate, micro plate, carbon nanotube, sensor chip etc. Just as known in the art, the solid carrier that film or plate etc. are smooth can be arranged bottom pit, groove, filter membrane etc.
In the present invention, magnetic bead can have the sphere diameter of about 25nm ~ about 1mm scope. In a preferred embodiment, magnetic bead has the diameter of about 50nm ~ about 10 �� m. The size of magnetic bead can be selected according to specific purposes.
In the present invention, the pearl being made up of the contour crosslinked spherical agarose of Sepharose has the diameter of about 24 ��m ~ about 165 �� m. Preferably, high crosslinked spherical sepharose 4B has the diameter of about 24 ��m ~ about 44 �� m. The size of high crosslinked spherical sepharose 4B can be selected according to specific purposes.
The example with the solid carrier of water repellent surface comprises can from polystyrene latex beads such as Polysciences, Warrington, PA or Spherotech, the goods that Liberville, IL buy.
Silicon-dioxide (SiO2)-process or silicon-dioxide (SiO2) example of solid carrier of base comprises the extraordinary magnetic silica pearl etc. can bought from Polysciences, Warrington, PA, it may be used for catching nucleic acid (such as DNA). Or, it is also possible to use the M-280 etc. that can buy from DynalBiotech.
The magnetic bead with hydrophilic surface can be used for catching the bacterial cell of propagation phase, nucleic acid and other composition. As the example of this magnetic bead, it is possible to list Polysciences, Warrington, the pearl (title: Biomag (registered trademark) carboxyl) that PA sells or BangsLaboratory, Inc., the name of Fishers, IN is called the pearl of MC02N/2928. Or, it is possible to use the M-270 etc. that DynalBiotech sells.
In a preferred embodiment of the present invention, described solid carrier is SJ modified silica-gel. The microarray solid support material (iPDMS film, see Chinese patent CN101265329A) of a kind of silicon rubber material of Suzhou consor thing Materials Co., Ltd exploitation.This kind of material is based on the conventional PDMS of biological study, add specific initiator composition (making this material realize surface-functionalized modification by surface initiated polymerization (SIP)) wherein, obtain through polyethylene glycol methacrylate-styrene polymer (poly (oligo (ethyleneglycol) methacrylate), pOEGMA) finishing again. SJ modified silica-gel has outstanding anti-protein non-specific adsorption (Nonspecificproteinadsorption, NPA) ability, non-specific protein absorption in can being detected by complicated protein immunization controls to close to " absolute 0 " level (being near or below the limit of detection of instrument), it is possible not only to exempt the trouble closed and repeatedly clean, it is also possible to improve the susceptibility of protein microarray with the use of stronger amplification of signal means. And the essence of its silicon rubber imparts the stronger mechanical property of this material and good operability. SJ modified silica-gel has successfully been applied to the combination assay microarray ELISA kit of 11 tumor markers compositions by the poly-company in Suzhou, achieving high-throughput and highly sensitive detection, demonstrating this kind of material is a kind of outstanding protein microarray solid support material. Meanwhile, this kind of material also has the adjustable characteristic of surface properties, it is possible to adjust its surface topography within the specific limits by the controlled modification reaction times.
The polypeptide of the present invention and the connection of solid carrier can adopt and well known to a person skilled in the art that the method for attachment of polypeptide and solid carrier carries out. such as, for the connection on protein/polypeptide and modified silica-gel surface, 1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimide [1-ethyl-3-(3-dimethylami-nopropyl) carbodiimide can be passed through, EDC] and N-hydroxy-succinamide (N-hydroxysuccinimide, NHS) reaction changes the carboxyl (-COOH) group on the macromolecular chain of modified silica-gel surface into activating group, this activating group can with protein/polypeptide on amino (-NH2) reaction thus realize being fixed on protein/polypeptide solid carrier surface (see Fig. 1).
Being not particularly limited for the concentration of the polypeptide of the present invention in the sampling liquid used during sample, those skilled in the art can conventionally select, it is preferable to 1 �� g ~ 1000 �� g/mL, it is more preferable to 10 �� g ~ 500 �� g/mL. In addition, the density that polypeptide for the present invention distributes on a solid support is not particularly limited, and those skilled in the art can conventionally select, it is preferable to 1 ~ 100 points/10mm2, it is more preferable to 5 ~ 50 points/10mm2��
The test kit of disease (such as malaria) that the detection means of the present invention may be used for detecting the disease (such as malaria) that causes of plasmodium or causes for the preparation of detection plasmodium.
The detection kit of the present invention
The present invention also relates to a kind of detection kit (detection kit of the present invention), and it comprises polypeptide or the detection means of the present invention. This detection kit is preferred for the disease (such as malaria) that detection plasmodium causes.
The detection means of the present invention or the polypeptide of the present invention are the important documents of the detection kit of the present invention. The detection kit of the present invention can also comprise:
1. the serum dilution prepared or serum dilution component solution: serum dilution, such as have the Sample dilution (production code member 070021-S2) of Sai Chi bio tech ltd, Beijing, Bo Weijia bio tech ltd, Zhengzhou add sample variable color Sample dilution (production code member bwj010103) etc.This serum dilution is used for dilute serum, and the serum of test kit detection to be diluted suitable multiple, such as 2 ~ 200 times, it is preferable that 10 ~ 100 times.
The detection kit of the present invention can also comprise:
2. concentrated washing lotion: after solid carrier surface hatches serum and ELIAS secondary antibody, need to wash, by washing lotion, antibody and the ELIAS secondary antibody that solid carrier surface does not combine. Concentrated washing lotion is such as the polysorbas20 aqueous solution of 1%, it may also be useful to time need to dilute 2 ~ 40 times, preferably 5 ~ 20 times.
The detection kit of the present invention can also comprise:
3. ELIAS secondary antibody solution: the plasmodium autoantibody in disease (such as malaria) patients serum that plasmodium causes can the polypeptide of the present invention on solid carrier (such as SJ modified silica-gel) be combined, two anti-can with antibodies, and two markers resisted can react with luminous substrate, thus send the light that can detect. ELIAS secondary antibody can be the goat anti-human igg of such as horseradish peroxidase-labeled. As ELIAS secondary antibody solution, it is possible to list the Goat anti human IgG (H+L) of the horseradish peroxidase-labeled that Beijing Bioisystech Co., Ltd of Zhong Shan Golden Bridge produces, production code member ZB-2304. The concentration of ELIAS secondary antibody in ELIAS secondary antibody solution is not particularly limited, it is possible to be such as 1ng ~ 1000ng/mL.
The detection kit of the present invention can also comprise:
4. luminescent solution component solution: luminescent solution can react with the horseradish peroxidase of mark in ELIAS secondary antibody so that reaction sends the chemical light that instrument can detect. Luminescent solution is mixed by two kinds of solution, is A liquid superoxol respectively, and B liquid luminol solution. Luminol (luminol,3-aminophthalic acid cyclic hydrazide) is only crossed by oxidizer treatment just can be luminous. Usually use the mixed aqueous solution of hydrogen peroxide and a kind of hydroxide bases as exciting agent. Under horseradish peroxidase enzyme catalytic, decomposing hydrogen dioxide solution is oxygen and water:
2H2O2��O2+2H2O
Luminol,3-aminophthalic acid cyclic hydrazide and oxyhydroxide generate a pairs of anion, the dioxygen oxidation that it can be decomposited by hydrogen peroxide when reacting, and product is an organo-peroxide. This superoxide is very unstable, decomposites nitrogen immediately, generates the 3-aminophthalic acid of excited state. During excited state to ground state transforms, the energy of release exists with the form of photon, and wavelength is positioned at the blue light part of visible ray. The SuperSignal ELISAFemtoMaximumSensitivitySubstrate of the example of luminescent solution component solution such as ThermoSeientific company, article No. 37074.
The detection kit of the present invention can also comprise:
5. one or more reaction cavity (such as Chinese patent Granted publication CN202054829U).
The detection kit of the present invention can also comprise:
6. the detection molecules (such as polypeptide, protein, nucleic acid etc.) of other diseases caused for detecting plasmodium (such as malaria).
The detection kit of the present invention can also comprise:
7. working instructions.
Embodiment
Hereinafter, by embodiment, the present invention is carried out more specific description, but it is not the restriction to the technology of the present invention scope. By the record of this specification sheets, those skilled in the art can be easy to the present invention is modified/changed, and these are included in the technical scope of the present invention.
The preparation of 1.30 peptides and confirmation
30 peptides used in embodiment have the aminoacid sequence shown in SEQIDNO:1, and by the synthesis of Shanghai Ji Er biochemical company limited, the sign of this polypeptide confirms to have synthesized described polypeptide by hydrogen spectrum and mass spectrum.Liquid phase chromatography detection purity: 96.33% (area normalization method): mass spectroscopy detection (ESI-MS): calculate molecular weight: 3513.95, test value 3512.8.
2. the preparation of detection means
Detection chip is taking SJ modified silica-gel (iPDMS film) as solid support material, is prepared from by a sample immobilized polypeptide solution thereon. Modified silica-gel be add in traditional polydimethylsiloxane material band olefin-terminal, the initiator of surface initiated polymerization, and it is fixed in the three-dimensional structure of polydimethylsiloxane by heat cross-linking (si-h bond bonding), obtain a kind of new material and SJ modified silica-gel. Its making processes is as shown in Figure 2.
A and B wherein is two components of polydimethylsiloxane, polydimethylsiloxane (Poly (dimethylsiloxane), Sylgard184) buy from Dow corning (DowCorning) company, comprise the two first siloxanes polymer precursor mixtures that liquid composition A(composition is metal platinum catalyzer and band vinyl) and crosslinking agent B (composition is the dimethyl siloxane precursor with vinyl and Si--H) two kinds of compositions. C is the initiator of end strips vinyl, is purchased from Hangzhou Dong Wei company. Polymer on finally modifying is that oligomeric ethylene glycol methacrylate monomer (Oligo (ethyleneglycol) methacrylate, hereinafter referred to as OEGMA, molecular weight Mw=526) is bought in Aldrich. Polydimethylsiloxane precursor A and crosslinking agent B are fully mixed with A:B:C=10:1:0.5 ratio with the initiator C of band vinyl end. Make transparent elastic silicone rubber by curing reaction, then carry out finishing by sip technique and can obtain SJ modified silica-gel. Experiment shows, the surface of SJ modified silica-gel have enough highdensity, by the initiator of covalently immobolization, its can pass through surface initiated polymerization (SIP) realize surface macromolecule modified. Poly (OEGMA) (polyethylene glycol methacrylate-styrene polymer) is used to carry out reacting the surface obtaining polyoxyethylene glycol (PolyethyleneGlycol, PEG) and modifying, it is achieved the ability of stronger anti-albumen non-specific adsorption.
The SJ modified silica-gel film made need to be kept in 4 DEG C of refrigerators.
Adopt brilliant core?PersonalArrayerTM16 people's point sample instrument prepares polypeptide microarray on modified silica-gel, and process is:
1) pre-treatment
By SJ modified silica-gel thin slice (15 �� 15mm2) be immersed in activation solution, after 30min, taking-up deionized water drip washing 3 times, blows dry with nitrogen, is used for some sample at once.
2) sample is put
Sampling liquid is diluted and gets well and transfer in the 384 corresponding micropores of orifice plate, 384 orifice plates of band sample are placed on point sample instrument Ji Tai, pretreated modified silica-gel thin slice are placed on the Ji Tai of point sample instrument simultaneously, carry out a sample at once. Point sample envrionment conditions is room temperature (25 DEG C), and humidity set is 50%. On the polypeptide microarray made, the some sample amount of each point is about 0.6nL, and sampling point radius is 200 ��m.
3) chemistry is fixing
The polypeptide microarray just made to be placed in climatic chamber (26 DEG C, 60% humidity) to fix at least 6h. Chemistry fixation procedure is as shown in Figure 3.
First the damping fluid point catching peptide molecule will be included on modified silica-gel film by point sample instrument, then damping fluid starts evaporation, catch peptide molecule and SJ modified silica-gel surface intimate contact and interact, by Chemical bond, the end-COOH of ploy (OEGMA) polymer and the NH of peptide molecule on modified silica-gel surface2Formed and stablize covalent linkage, and then the peptide molecule having chemically reactive is fixed on SJ modified silica-gel surface.
5) assemble
The polypeptide microarray of fixing 6h must in-built at two days prepare. First by back of the body glue, SJ modified silica-gel thin slice is attached on special reaction column, covers reaction cavity. A reactor is made up of two reaction columns and a reaction cavity.
6) preserve
The polypeptide microarray assembled, it is necessary to vacuumize sealing, is kept in the refrigerator of 4 DEG C, for subsequent use.
3. detect by detection means
Checking procedure
1, before starting detection, concentrated cleaning solutions is added purified water in the ratio of 1:10 or distilled water dilutes, directly use after dilute. Use shifting liquid rifle that 2mL scavenging solution is added to chip surface, soak chip 3 minutes, ensure that chip surface is fully wet out.
2, serum sample Sample dilution to be measured is mixed even according to 1:40 dilution.
3, abandon soak chip scavenging solution, when chip surface is completely moistening, each serum sample draw 200 �� L dilute after serum join in chip reactor.
4, chip reactor is put into chip permanent seat, it is put on shaking table, open shaking table, frequency 150 revs/min, incubated at room 30 minutes.
5, the serum sample in chip reactor is abandoned, with 15mL washing lotion cleaning reaction cavity and chip surface 3 times.
6, after having cleaned, each chip reactor adds 200 �� L enzyme labelled antibody solution respectively, chip reactor is put into chip permanent seat, is put on shaking table, opens shaking table, frequency 150 revs/min, incubated at room 30 minutes.
7, the enzyme labelled antibody solution in chip reactor is abandoned, with 15mL washing lotion cleaning reaction cavity and chip surface 3 times.
8, after having cleaned, taking off reaction cavity, each chip surface adds 15 �� L luminous substrate liquid respectively, makes luminescent solution can be laid on chip surface uniformly.
9, the chip adding luminescent solution is placed in gel imaging instrument chemiluminescence imaging, and sentence read result.
Serum and other diseases patients serum's sample of the malaria patient that subtertian malaria causes are provided by chain hospital. Serum is transported by related personnel with parcels such as ice cube/dry ice or is handed to laboratory soon.
Negative control have PBS (namely in the 3rd step need not sera incubation to be measured, and hatch by PBS solution, all the other steps are identical) comparison, the comparison of serum dilution, and the comparison of negative patient (referring to healthy people and non-malaria patient) serum.
The spot sample mode of polypeptide microarray is as shown in Figure 4. Wherein, the sample of 17 points of trilateral is human IgG, as the locating point of experiment; The sample polypeptides of star point is the polypeptide SEQIDNO:1 of the present invention, and it is malaria autoantigen protein polypeptide, malaria patients' serum can be produced response; Circular dot sample be other malaria autoantigen protein polypeptide, as the Testing index (these polypeptide have response to illustrate in detection serum have malaria autoantibody) of experiment.
Experimental result is as shown in Fig. 5 ~ 6. Wherein, Fig. 5 shows the detected result of negative control, and only the sample of the point shown in trilateral has response. Fig. 6 shows the detected result of malaria disease human serum, and the sample of trilateral, star point and circle has response. It should be noted that, instrument records signal value from low to high, and corresponding signal point color is by black and white gradual change.
SEQUENCELISTING
<110>Chinese Academy of Sciences Suzhou nanotechnology and nano bionic institute
<120>polypeptide, the detection means comprising this polypeptide and detection kit
<130>S36-78
<160>1
<170>PatentInversion3.5
<210>1
<211>30
<212>PRT
<213>artificial sequence
<220>
<223>malaria antigen polypeptide
<400>1
LysAspValIleGluGlnGluIleValSerGluGluValAsnGluLys
151015
AspThrLysAsnAsnAspLysLysIleGlyLysArgValLys
202530
Claims (9)
1. a peptide species, its aminoacid sequence as shown in SEQIDNO:1, that is: KDVIEQEIVSEEVNEKDTKNNDKKIGKRVK.
2. a detection means, comprising:
Solid carrier, and
The polypeptide according to claim 1 being connected on this solid carrier.
3. detection means according to claim 2, wherein, described solid carrier is SJ modified silica-gel.
4. a detection kit, it comprises the detection means described in polypeptide according to claim 1 or Claims 2 or 3.
5. the antibody of anti-polypeptide according to claim 1.
6. the purposes of polypeptide according to claim 1 in the test kit of disease caused for the preparation of detection plasmodium or detection means.
7. purposes according to claim 6, wherein, described disease is malaria.
8. purposes described in claim 6 or 7, wherein, described plasmodium is subtertian malaria.
9. encode the nucleic acid of polypeptide according to claim 1, the expression vector comprising described nucleic acid, the host cell that imported described expression vector.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410628804.6A CN105646689A (en) | 2014-11-10 | 2014-11-10 | Polypeptide and detection device and detection kit containing polypeptide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410628804.6A CN105646689A (en) | 2014-11-10 | 2014-11-10 | Polypeptide and detection device and detection kit containing polypeptide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105646689A true CN105646689A (en) | 2016-06-08 |
Family
ID=56483519
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| CN201410628804.6A Withdrawn CN105646689A (en) | 2014-11-10 | 2014-11-10 | Polypeptide and detection device and detection kit containing polypeptide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105646689A (en) |
-
2014
- 2014-11-10 CN CN201410628804.6A patent/CN105646689A/en not_active Withdrawn
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