CN105638739A - Pesticide composition for preventing and treating fungal disease - Google Patents
Pesticide composition for preventing and treating fungal disease Download PDFInfo
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- CN105638739A CN105638739A CN201610031394.6A CN201610031394A CN105638739A CN 105638739 A CN105638739 A CN 105638739A CN 201610031394 A CN201610031394 A CN 201610031394A CN 105638739 A CN105638739 A CN 105638739A
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- Prior art keywords
- tebuconazole
- agent
- composition pesticide
- bacillus subtilis
- dispersant
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
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- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Biotechnology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a pesticide composition for preventing and treating a fungal disease. Tebuconazole and bacillus subtilis are used as effective ingredients, the weight ratio of tebuconazole to bacillus subtilis is 10:1-1:4, additives and filler acceptable for pesticide are adopted, and a dispersing agent, a wetting agent, a disintegrating agent and/or a binding agent are adopted as the additives. The effective ingredients, tebuconazole and bacillus subtilis in the pesticide composition account for 1-5% of the product by weight. The drug form is a water dispersing agent. The composition has the advantages of not influencing the seed germinating stage of crops and having a synergistic effect of preventing and treating the fungal disease.
Description
Technical field
The invention belongs to technical field of pesticide, particularly to a kind of composition pesticide preventing and treating fungal disease, wherein effective ingredient is Tebuconazole and bacillus subtilis.
Background technology
Tebuconazole; chemical name: (RS)-1-(4-chlorphenyl)-4; 4-dimethyl-3-(1H-1; 2; 4 triazol-1-yl methyl) penta-3-alcohol; being a kind of efficient, wide spectrum, interior absorption triazole type sterilization pesticide, have protection, treat, root out three zones, fungicidal spectrum is wide, the lasting period is long. Tebuconazole is mixed even with seed stirring before being mainly used in crop seeding, then sows seed, to reach preventing and treating farm crop fungus infection effect, is particularly useful for preventing and treating loose smut of wheat. Dressing seed crop seeds is sprouted due to Tebuconazole and have inhibitory action, causing crop growth cycle stretch-out, thus affecting proportion of crop planting cost.
Bacillus subtillis is a kind of gram-positive bacterium being distributed widely in various different living environment, can sprout in producing born of the same parents, at the surface ubiquity of soil and plant, simultaneously or a kind of endophyte common in plant, to person poultry harmless, free from environmental pollution. Bacillus subtilis is applied to prevent and treat wheat scab relevant report; the preliminary study of wheat scab " the bacillus subtilis bacterial strain A014 bacterial strain prevent and treat " delivered by force such as Wang Yaping, Liu Yi is pointed out; gibberella saubinetii is had stronger bacteriostasis by bacillus subtilis bacterial strain A014; and have certain protection effect; make infection process rate reduce by 50%, can protect the wheat head not by the extension of infection process or restriction fringe portion pathogenic bacteria preferably.
At present, using Tebuconazole, bacillus subtilis as the preventing and treating crops germination period fungal infection of effective ingredient but without relevant report, both are optionally combined crops germination period without impact also to there is no prior art enlightenment, and prevent and treat and have potentiation on pathogenic bacteria.
Summary of the invention
It is an object of the invention to, it is provided that a kind of composition pesticide preventing and treating fungal disease, there is high-efficiency low-toxicity, reduce the using dosage of Tebuconazole and do not affect the advantages such as crops germination period.
To achieve these goals, the technical solution used in the present invention is:
A kind of composition pesticide preventing and treating fungal disease, including effective ingredient Tebuconazole and Bacillus subtillis, wherein Tebuconazole is 10:1��1:4 with the weight ratio of Bacillus subtillis, and acceptable auxiliary agent and filler on pesticide.
The weight ratio of described effective ingredient Tebuconazole and Bacillus subtillis is 5:1��1:2, it is preferred to 2:1.
Described auxiliary agent is dispersant, wetting agent, disintegrating agent and/or binding agent, the optional dodecyl sodium naphthalene sulfonate of wherein said dispersant or sodium methylene bis-naphthalene sulfonate, the optional sodium lauryl sulphate of wetting agent or dodecyl sodium sulfate, disintegrating agent may select carbamide, and binding agent may select xanthan gum.
Described filler may select Kaolin.
In described composition pesticide, shared by each component, mass percent is: effective ingredient Tebuconazole and Bacillus subtillis account for 1��5%, dispersant accounts for 3��12%, wetting agent accounts for 2��8%, disintegrating agent accounts for 1��8%, binding agent accounts for 0.001��5%, and surplus is filler.
In described composition pesticide percent mass shared by each component preferably than accounting for 2% for: effective ingredient Tebuconazole and Bacillus subtillis, dispersant accounts for 8%, wetting agent accounts for 5%, disintegrating agent accounts for 4.5%, binding agent accounts for 3%, surplus is filler.
Invention also provides the method preparing this composition pesticide, in proportion effective ingredient Tebuconazole is mixed together with Bacillus subtillis, dispersant, wetting agent, disintegrating agent and filler component, pulverizes; Add binding agent, obtain granulation materials, granulation materials are granulated, are drying to obtain, wherein granulate and dry employing fluidized bed process.
Described fluidized bed process is granulated and dry fluid is noble gas.
Described fluidized bed process is granulated and dry fluid temperature (F.T.) is 30��60 DEG C.
Described composition pesticide is water dispersant.
The present invention has the following advantages and significant technique effect:
(1) medicament high-efficiency low-toxicity, compares Tebuconazole, and the toxicity of people and animals is substantially reduced by medicament, it is to avoid the risk of production and application person poultry poisoning.
(2) active component Tebuconazole and Bacillus subtillis Synergistic, use with in the reasonable scope, can reduce the amount of application of each single dose, advantageously reduces drug cost and reduces environmental pollution.
(3) avoid because Tebuconazole suppresses seed germination to extend the agronomic crop seed germination phase, thus reducing proportion of crop planting cost.
Detailed description of the invention
Embodiments of the present invention are described in detail below in conjunction with embodiment.
One, indoor joint toxicity measuring test
The indoor joint toxicity measuring test of Tebuconazole, bacillus subtilis and mixed agent thereof, active constituent content gradient series 5 concentration of dilution are pressed with Tebuconazole, bacillus subtilis and mixed agent thereof, compare with sterilized water, test with fusarium graminearum for for examination antibacterial, blank is set, Tebuconazole, bacillus subtilis single dose and mixture preparation thereof are adopted dip method, carries out toxicity test and do statistical analysis.
Evaluate the potentiation of pharmacy mix with the co-toxicity coefficient method (CTC) of the abundant method of Sun Yun, namely CTC��80 are antagonism, and 80<CTC<120 is summation action, and CTC>=120 are potentiation. Statistical result is as shown in table 1:
Table 1: Tebuconazole, bacillus subtilis and mixed agent thereof the Toxicity Determination table to fusarium graminearum
Showing according to table 1 measurement result, when the mixed ratio of Tebuconazole and bacillus subtilis is 12:1, co-toxicity coefficient (CTC) is 114.21, and both mixtures are summation action; When the mixed ratio of Tebuconazole and bacillus subtilis is 1:8, co-toxicity coefficient (CTC) is less than 80, and both mixtures are antagonism; When the mixed ratio of Tebuconazole and bacillus subtilis is 10:1��1:4 scope, co-toxicity coefficient (CTC) is all higher than 120, and namely potentiation is obvious, and wherein 2:1 potentiation is best.
Two, product embodiments
Cfu/ gram of bacillus subtilis water dispersant of embodiment 1:1% Tebuconazole 100,000,000
Formula: the weight ratio according to Tebuconazole with bacillus subtilis is 10:1, weighs Tebuconazole crude drug 0.91Kg, the 100000000 cfu/ gram of former powder 0.09Kg of bacillus subtilis, dodecyl sodium naphthalene sulfonate 8Kg, sodium lauryl sulphate 5Kg, carbamide 4.5Kg, xanthan gum 3Kg, Kaolin complements to 100Kg.
Cfu/ gram of bacillus subtilis water dispersant of embodiment 2:2% Tebuconazole 100,000,000
Formula: the weight ratio according to Tebuconazole with bacillus subtilis is 2:1, weighs Tebuconazole crude drug 1.33Kg, the 1000000000 cfu/ gram of former powder 0.67Kg of bacillus subtilis, dodecyl sodium naphthalene sulfonate 8Kg, sodium lauryl sulphate 5Kg, carbamide 4.5Kg, xanthan gum 3Kg, Kaolin complements to 100Kg.
Cfu/ gram of bacillus subtilis water dispersant of embodiment 3:5% Tebuconazole 100,000,000
Formula: the weight ratio according to Tebuconazole with bacillus subtilis is 1:4, weighs Tebuconazole crude drug 1.00Kg, the 100000000 cfu/ gram of former powder 4.00Kg of bacillus subtilis, dodecyl sodium naphthalene sulfonate 8Kg, sodium lauryl sulphate 5Kg, carbamide 4.5Kg, xanthan gum 3Kg, Kaolin complements to 100Kg.
Cfu/ gram of bacillus subtilis water dispersant of embodiment 4:2% Tebuconazole 100,000,000
Formula: the weight ratio according to Tebuconazole with bacillus subtilis is 1:2, weighs Tebuconazole crude drug 0.67Kg, the 500000000 cfu/ gram of former powder 1.33Kg of bacillus subtilis, sodium methylene bis-naphthalene sulfonate 12Kg, sodium lauryl sulphate 2Kg, carbamide 8Kg, xanthan gum 0.001Kg, Kaolin complements to 100Kg.
Cfu/ gram of bacillus subtilis water dispersant of embodiment 5:2% Tebuconazole 100,000,000
Formula: the weight ratio according to Tebuconazole with bacillus subtilis is 5:1, weighs Tebuconazole crude drug 1.67Kg, the 500000000 cfu/ gram of former powder 0.33Kg of bacillus subtilis, dodecyl sodium naphthalene sulfonate 2Kg, dodecyl sodium sulfate 8Kg, carbamide 1Kg, xanthan gum 5Kg, Kaolin complements to 100Kg.
Cfu/ gram of bacillus subtilis water dispersant of embodiment 6:1% Tebuconazole 100,000,000
Formula: the weight ratio according to Tebuconazole with bacillus subtilis is 2:1, weighs Tebuconazole crude drug 0.67Kg, the 1000000000 cfu/ gram of former powder 0.33Kg of bacillus subtilis, dodecyl sodium naphthalene sulfonate 8Kg, sodium lauryl sulphate 5Kg, carbamide 4.5Kg, xanthan gum 3Kg, Kaolin complements to 100Kg.
Cfu/ gram of bacillus subtilis water dispersant of embodiment 7:5% Tebuconazole 100,000,000
Formula: the weight ratio according to Tebuconazole with bacillus subtilis is 2:1, weighs Tebuconazole crude drug 3.33Kg, the 1000000000 cfu/ gram of former powder 1.67Kg of bacillus subtilis, dodecyl sodium naphthalene sulfonate 8Kg, sodium lauryl sulphate 5Kg, carbamide 4.5Kg, xanthan gum 3Kg, Kaolin complements to 100Kg.
Preparation method: by other component will be mixed together, pulverize except bonding agent described in above-described embodiment 1-7; Add binding agent, obtain granulation materials, adopt fluid bed process granulate and be drying to obtain water dispersant granulation materials.
Three, products application embodiment
Adopt product of the present invention, do preventing and treating gibberella saubinetii field efficacy experiment, often process in triplicate, the repetition of one, every community, adopt the group arrangement of random district, plot area 20m2. Before sowing, wheat seed sample adds water and is uniformly mixed, and sows immediately.
Embodiment 1 to embodiment 7 products obtained therefrom carries out the preventing and treating of gibberella saubinetii, and test processes and is provided that
Process 1: 1% cfu/ gram of bacillus subtilis water dispersant of Tebuconazole 100,000,000 of embodiment 1, amount of application is 2.2g/kg wheat seed.
Process 2: 2% cfu/ gram of bacillus subtilis water dispersant of Tebuconazole 100,000,000 of embodiment 2, amount of application is 0.75g/kg wheat seed.
Process 3: 5% cfu/ gram of bacillus subtilis water dispersant of Tebuconazole 100,000,000 of embodiment 3, amount of application is 2.5g/kg wheat seed.
Process 4: 2% cfu/ gram of bacillus subtilis water dispersant of Tebuconazole 100,000,000 of embodiment 4, amount of application is 1.35g/kg wheat seed.
Process 5: 2% cfu/ gram of bacillus subtilis water dispersant of Tebuconazole 100,000,000 of embodiment 5, amount of application is 0.9g/kg wheat seed.
Process 6: 1% cfu/ gram of bacillus subtilis water dispersant of Tebuconazole 100,000,000 of embodiment 6, amount of application is 1.5g/kg wheat seed.
Process 7: 5% cfu/ gram of bacillus subtilis water dispersant of Tebuconazole 100,000,000 of embodiment 7, amount of application is 0.3g/kg wheat seed.
Processing 8:2.0% Likeming Gel, amount of application is 1.5g/kg wheat seed.
Processing the cfu/ gram of former powder of bacillus subtilis of 9:5 hundred million, amount of application is 5g/kg wheat seed.
Process 10: blank.
After above-mentioned process is used, observe the germination period of Semen Tritici aestivi, germination percentage.
Before results, loose smut of wheat situation being added up, final statistical result is as shown in table 2:
Table 2: process 1-10 sample wheat germination period and prevent and treat wheat scab statistical effect table
Show according to table 2 result, composition pesticide of the present invention is when using effective ingredient Tebuconazole and the medicament that bacillus subtilis weight ratio is 20:1��5:1 more suitable than the dosing of every mu of use single active ingredient for every mu, and wheat seed germinating phase ratio is used alone Tebuconazole substantially to be shortened; Prevent and treat wheat smut effect ratio and be used alone Tebuconazole or bacillus subtilis is effective, thus wheat yield is significantly improved; Semen Tritici aestivi sprout rate slightly improves. Comprehensive data above, best results is the medicament of 2:1 for effective ingredient Tebuconazole and bacillus subtilis weight ratio.
The above, be only to presently preferred embodiments of the present invention, the present invention not do the restriction of other forms, and any those skilled in the art are changed or be modified as the equal Equivalent embodiments changed possibly also with the technology contents of the disclosure above. Every without departing from the present invention program content, the technical spirit of the foundation present invention, to any simple modification made for any of the above embodiments, equivalent variations and remodeling, all falls within protection scope of the present invention.
Claims (10)
1. the composition pesticide preventing and treating fungal disease, it is characterised in that include effective ingredient Tebuconazole and Bacillus subtillis, wherein the weight ratio of Tebuconazole and Bacillus subtillis is 10:1��1:4, and acceptable auxiliary agent and filler on pesticide.
2. composition pesticide according to claim 1, it is characterised in that the weight ratio of described effective ingredient Tebuconazole and bacillus subtilis is 5:1��1:2.
3. composition pesticide according to claim 2, it is characterised in that the weight ratio of described effective ingredient Tebuconazole and bacillus subtilis is 2:1.
4. the composition pesticide according to claims 1 to 3 any one, it is characterized in that, on described pesticide, acceptable auxiliary agent is dispersant, wetting agent, disintegrating agent and/or binding agent, described dispersant is dodecyl sodium naphthalene sulfonate or sodium methylene bis-naphthalene sulfonate, described wetting agent is sodium lauryl sulphate or dodecyl sodium sulfate, described disintegrating agent is carbamide, and described binding agent is xanthan gum; On described pesticide, acceptable filler is Kaolin.
5. composition pesticide according to claim 4, it is characterized in that, in composition pesticide, shared by each component, percentage by weight is: effective ingredient Tebuconazole and Bacillus subtillis account for 1��5%, dispersant accounts for 3��12%, wetting agent accounts for 2��8%, disintegrating agent accounts for 1��8%, binding agent accounts for 0.001��5%, and surplus is filler.
6. composition pesticide according to claim 5, it is characterized in that, in composition pesticide, shared by each component, percentage by weight is: effective ingredient Tebuconazole and Bacillus subtillis account for 2%, dispersant accounts for 8%, wetting agent accounts for 5%, disintegrating agent accounts for 4.5%, binding agent accounts for 3%, and surplus is filler.
7. the method for the composition pesticide prepared as described in claim 1��6 any one, it is characterized in that, the method is in described ratio, effective ingredient Tebuconazole to be mixed together with Bacillus subtillis, dispersant, wetting agent, disintegrating agent and filler component, pulverized; Add binding agent, obtain granulation materials, adopt fluidized bed process to granulate granulation materials, dry, to obtain final product.
8. method according to claim 7, it is characterised in that described fluidized bed process is granulated and the dry fluid adopted is noble gas.
9. method according to claim 8, it is characterised in that described fluidized bed process is granulated and the dry fluid temperature (F.T.) adopted is 30��60 DEG C.
10. method according to claim 7, it is characterised in that it is water dispersant that the method makes the dosage form of composition pesticide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610031394.6A CN105638739A (en) | 2016-01-19 | 2016-01-19 | Pesticide composition for preventing and treating fungal disease |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610031394.6A CN105638739A (en) | 2016-01-19 | 2016-01-19 | Pesticide composition for preventing and treating fungal disease |
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| CN105638739A true CN105638739A (en) | 2016-06-08 |
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| CN201610031394.6A Pending CN105638739A (en) | 2016-01-19 | 2016-01-19 | Pesticide composition for preventing and treating fungal disease |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102731217A (en) * | 2012-07-20 | 2012-10-17 | 褚贵德 | Rice seedling growing gasket |
| WO2014147534A1 (en) * | 2013-03-20 | 2014-09-25 | Basf Corporation | Synergistic compositions comprising a bacillus subtilis strain and a pesticide |
-
2016
- 2016-01-19 CN CN201610031394.6A patent/CN105638739A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102731217A (en) * | 2012-07-20 | 2012-10-17 | 褚贵德 | Rice seedling growing gasket |
| WO2014147534A1 (en) * | 2013-03-20 | 2014-09-25 | Basf Corporation | Synergistic compositions comprising a bacillus subtilis strain and a pesticide |
Non-Patent Citations (4)
| Title |
|---|
| 杨义 等: "芽孢杆菌Bv22菌株对茉莉花白绢病的防治效果试验", 《广西农业科学》 * |
| 杨阿丽 等: "生物药剂和化学药剂对苹果树腐烂病菌的抑制效应", 《中国农学通报》 * |
| 陈志谊 等: "戊唑醇和枯草芽孢杆菌协同作用防治蚕豆枯萎病及增效机理初探", 《农药学学报》 * |
| 韩丽 等: "枯草芽孢杆菌B26与化学药剂协同防治木材蓝变", 《东北林业大学学报》 * |
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