CN105638407A - Method for acer palmatum gold thread twig cuttage - Google Patents
Method for acer palmatum gold thread twig cuttage Download PDFInfo
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- CN105638407A CN105638407A CN201511023577.5A CN201511023577A CN105638407A CN 105638407 A CN105638407 A CN 105638407A CN 201511023577 A CN201511023577 A CN 201511023577A CN 105638407 A CN105638407 A CN 105638407A
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- cutting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- Environmental Sciences (AREA)
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Abstract
The invention discloses a method for acer palmatum gold thread twig cuttage. The method comprises the steps of cuttage bed preparation, cutting slip collecting and processing, cuttage, management after cuttage. Gold thread twigs are utilized for cuttage inside a greenhouse, and high-quality gold thread nursery stocks are obtained through early-stage cutting slip clipping and hormone treatment and control over relative humidity, relative temperature and illumination of the growing environment of the later stage and the water content of a substrate. According to the method for cuttage, the culture rooting percentage is high, the survival rate of transplanting is high, the features of the good strain can be well kept, and the method can be directly applied to actual production and has the good economic, social and ecological benefits.
Description
Technical field
The present invention relates to technical field of plant asexual propagation, a kind of method being specifically related to Acer palmatum gold thread softwood cutting propagation.
Background technology
Acer palmatum (Acerpalmatum) has another name called Japanese maple, belongs to Aceraceae Acer L defoliation small arbor or shrub. Viewing and admiring color leaf plant as excellent, the applicating history in China gardens is long, and the application in city trees and shrubs now is then more general. Gold thread (Acerpalmatum ' Osakazuki ') is one of Acer palmatum gardening mutational variety. Deciduous tree, the speed of growth is grown up with plant and is slowed down, its blade keratin, and when liquid manure is suitable, blade is not easily burnt. Spring, blade was that yellowish green colour band is micro-red, and summer is bottle green, presents strong peony autumn, is compared to " shrub of burning ". The one that gold thread (Acerpalmatum ' Koto-no-ito ') is the numerous variation cultivar of Acer palmatum. It addition, gold thread young leaves is peony when just growing but rapidly goes to green; Summer, blade was gradually become bottle green by yellow green; Autumn, blade was then present pale yellow, orange, orange and red mixing, was very attractive in appearance, and plant is ornamental value more. In recent years, along with the raising of people's living standard, color leafed plants is increasingly favored by people. The concern of people makes gold thread become the focus viewed and admired.
The normal cutting propagation propagation method of current gold thread is long for breeding cycle, and rooting rate is unstable, and root system is less-developed, shows as that main root is obvious, lateral root development is bad, is unfavorable for large-scale production and the exploitation application of gold thread cottage propagation. Therefore, the effective way exploring an energy Fast-propagation gold thread is problem demanding prompt solution.
Summary of the invention
In view of this, the application provides a kind of method of Acer palmatum gold thread softwood cutting propagation, and described method cultivation period is short, root system is good, survival rate is high.
For solving above technical problem, technical scheme provided by the invention is a kind of method of Acer palmatum gold thread softwood cutting propagation, including management operation after cutting bed preparation, cutting collection and process, cuttage, cuttage, comprises the following steps:
A, using Vermiculitum, perlite as cutting medium, described cutting medium is placed in cuttage seedbed; And to described cutting medium disinfection;
B, on scion-seed tree, gather raw semi-lignified branch then, described semi-lignified branch is cut into cutting, as cuttage fringe bar, and to described cutting disinfection; Cutting base portion after sterilization is soaked 11-13h in the process solution of (50-150) mg/LNAA+ (250-350) mg/LIBA;
C, the cutting cuttage obtained by described step B are to described cuttage seedbed;
D, the cuttage seedbed after step C cuttage is placed under first condition and every 57 days, described cuttage seedbed is carried out disinfection; Described first condition is relative air humidity 80% 95%, air themperature 25 30 DEG C, intensity of illumination 3000 5000lx.
Further, the formula material volume ratio proportioning of described cutting medium: Vermiculitum: perlite is 1:2, mix standby.
Further, described step A concentration is that the potassium permanganate solution of 5% is to substrate disinfection.
Further, gathering cutting in the annual mid-August the first tenday period of a month in May in described step B, the diameter of the described fringe bar of collection is 0.3cm-0.5cm.
Further, semi-lignified branch is cut into cutting by described step B method particularly includes:
Described semi-lignified branch cutting into the cutting of 8cm 12cm, removes taper children's tender part during shearing, the upper clip of cutting is horizontal section, lower clip is angle of inclination is the short inclined-plane of 30 45 ��, lower clip distance joint 1 1.5cm, cutting upper end retains 23 leaves, cuts the blade of lower end.
Further, cutting is carried out disinfection by described step B method particularly includes: described cutting is soaked 10 15min in 700 1200 times of solution of the carbendazim that effective ingredient is 80%.
Further, cutting is carried out disinfection by described step B method particularly includes: 10 15min are soaked in the two ends that described cutting has wound in 800 1000 times of solution of the carbendazim that effective ingredient is 80%.
Further, the cutting base portion after sterilization is soaked 12h by described step B in the process solution of (50-150) mg/LNAA+ (250-350) mg/LIBA.
Further, the process solution of described step B is: the solution of 100mg/LNAA+300mg/LIBA.
Further, by cutting cuttage to the cuttage seedbed getting out substrate in described step C method particularly includes:
During cuttage, it is not overlapping that cutting density is that described cutting blade connects, and the cuttage degree of depth is the 1/3 1/2 of whole described cutting, by the cutting medium compacting around described cutting after cuttage, water immediately 1 time permeable, make described cutting and cutting medium combine closely simultaneously;
Wherein, described cutting density is spacing in the rows 4cm 5cm, and line-spacing is 4cm 6cm; The described cuttage degree of depth is 4cm 6cm.
Further, line-spacing is 5cm.
Further, described step D is by described step C cuttage post processing method particularly includes:
Described cuttage seedbed is carried out disinfection 1 time by the potassium permanganate solution adopting concentration to be 5% every 57 days after cuttage; Described cuttage seedbed is made to be maintained under the environment of intensity of illumination 2500 3000lx and water after cuttage in 40 60 days.
Compared with prior art, the invention has the beneficial effects as follows:
Cuttage breeding method of the present invention is compared with type of seeding, have simple, effect high inexpensive, reproduction speed is fast, breeding coefficient is high a little, need not sowing saving seed and it is bred primary growth speed after planting and substantially increases, increment is big, strong adaptability.
Utilizing the nursery stock that cuttage breeding method of the present invention is bred to have the amount of taking root big, the obvious feature of lateral root development, after flourishing side root is conducive to planting, seedling root recovers rapidly, grows, is conducive to raising survival rate.
Cuttage breeding method of the present invention, cultivates rooting rate height, transplanting survival rate height, it is possible to the good feature keeping excellent strain, it is possible to directly apply to actual production, have good economic benefit, social benefit and ecological benefits.
Detailed description of the invention
In order to make those skilled in the art be more fully understood that technical scheme, below in conjunction with specific embodiment, the present invention is described in further detail.
The method of Acer palmatum gold thread softwood cutting propagation of the present invention, including management operation after cutting bed preparation, cutting collection and process, cuttage, cuttage. Adopt of the present invention cuttage breeding method cuttage Acer palmatum gold thread branch and apply its cultivate Acer palmatum gold thread nursery stock detailed process as follows:
1, the preparation in cuttage seedbed
Cutting medium, as cutting medium, is placed in cuttage seedbed by the mixture being made up of the Vermiculitum that volume ratio is 1:2 and perlite; Cuttage concentration the last week is that the potassium permanganate solution of 5% is to substrate disinfection.
2, cutting collection and process
First gather on the scion-seed tree of the annual mid-August the first tenday period of a month in May and give birth to semi-lignified branch then as cuttage cutting; Branch thick ranges for 0.3 0.5cm. Preferably, carry out adopting fringe time not dry at cloudy day or fine day dew in morning, and gather before cutting and gather in cutting, it is necessary to sampling instrument adopts the ethanol of 70%-80% carry out disinfection; More excellent, sampling instrument adopt the ethanol of 75% carry out disinfection. After gathering cutting, fringe bar is put into equipped with in the bucket of clear water.
Then the cutting collected is pruned: the fringe bar of semi-lignified is cut into the cutting of 8 12cm, taper children's tender part is removed during shearing, retain 2-4 lateral bud of cutting, the upper clip of cutting is horizontal section, distance lateral bud 1.5-2.0cm, lower clip is angle of inclination is the short inclined-plane of 30 45 ��, lower clip distance joint 1 1.5cm, cutting upper end retains 23 leaves, cuts the blade of lower end.
Subsequently to the cutting disinfection after pruning: cutting is soaked 10 15min in the carbendazim solution that effective ingredient is 80%, taking-up clear water rinses 2-3 time, removes medicine unnecessary on cutting stand-by. Preferably, 10 15min are soaked in two ends cutting with wound in 700 1200 times of solution of the carbendazim that effective ingredient is 80%; More preferably, 10 15min are soaked in two ends cutting with wound in 800 1000 times of solution of the carbendazim that effective ingredient is 80%.
Finally cutting is carried out HORMONE TREATMENT: cutting base portion is soaked in the process solution of (50-150) mg/LNAA+ (250-350) mg/LIBA 11-13h, preferably, cutting base portion is soaked in the process solution of 100mg/LNAA+300mg/LIBA 12h, then takes out and carry out cuttage immediately;
3, cuttage
Carrying out cuttage: will be inserted on the cuttage seedbed getting out substrate through hormone-treated cutting, it is not overlapping that cutting density is that cutting blade connects, and the cuttage degree of depth is the 1/3 1/2 of whole cutting. Preferably, cutting density is spacing in the rows 4 5cm, and line-spacing is 4 6cm; The cuttage degree of depth is 4 6cm; More excellent, cuttage line-spacing is 5cm. By the cutting medium compacting around cutting after cuttage, water immediately 1 time permeable, make cutting and cutting medium combine closely simultaneously.
4, manage after cuttage
Cuttage seedbed after cuttage is placed in relative air humidity 80% 95%, so that cutting can be taken root when air themperature 25 30 DEG C, intensity of illumination 3000 5000lx.
Taking root, period needs cutting is carried out disease control: the disinfectant of the potassium permanganate solution adopting concentration to be 5% every 57 days after cuttage 1 time. Under the environment of intensity of illumination 2500 3000lx, liquid manure is enriched in 40 60 days after cuttage.
In being embodied as, the present invention is using the cottage propagation of Acer palmatum gold thread as an application of the present invention.
Matched group 1 existing seed propagation Acer palmatum gold thread nursery stock
Adopt seed propagation Acer palmatum gold thread to first have to be loosened in nursery, and mix fertilizer, then seed sowed and suitably soak so that it is sprouted. It is carried out the operation such as illumination, fertilising and obtains seedling by the later stage, then transplants and makes it take root.
Experimental group 1 cottage propagation of the present invention Acer palmatum gold thread nursery stock
Cutting cuttage May the first tenday period of a month-mid-August, spray in warmhouse booth in full exposure batch (-type), cutting medium, as cutting medium, is placed in cuttage seedbed by the mixture being made up of the Vermiculitum that volume ratio is 1:2 and perlite; The potassium permanganate solution that cuttage adopts concentration to be 5% the last week is to substrate disinfection.
Gather fringe bar be May the first tenday period of a month-mid-August, the branch base portion profit long robust growth of clip 8��12cm of trying one's best on scion-seed tree without pest and disease damage, semi-lignified twig as cuttage cutting. Removing taper children's tender part during shearing, retain 2-4 lateral bud of cutting, the upper clip of cutting is horizontal section, distance lateral bud 1.5-2.0cm, lower clip is angle of inclination is the short inclined-plane of 30 45 ��, lower clip distance joint 1 1.5cm, cutting upper end retains 23 leaves, cuts the blade of lower end. 10 15min are soaked in the two ends then cutting with wound in 700 1200 times of solution of the carbendazim that effective ingredient is 80%. Finally cutting base portion is soaked in the process solution of (50-150) mg/LNAA+ (250-350) mg/LIBA 11-13h, takes out and carry out cuttage immediately;
To be inserted on the cuttage seedbed getting out substrate through hormone-treated cutting, it is not overlapping that cutting density is that cutting blade connects, and the cuttage degree of depth is the 1/3 1/2 of whole cutting. Preferably, cutting density is spacing in the rows 4 5cm, and line-spacing is 4 6cm; The cuttage degree of depth is 4 6cm; More excellent, cuttage line-spacing is 5cm. By the cutting medium compacting around cutting after cuttage, water immediately 1 time permeable, make cutting and cutting medium combine closely simultaneously.
Cuttage seedbed after cuttage keeps relative air humidity 80% 95%, so that cutting can be taken root when air themperature 25 30 DEG C, intensity of illumination 3000 5000lx. Taking root, period needs cutting is carried out disease control: every the disinfectant that 57 days spray concentrations are the potassium permanganate solution of 5% 1 time after cuttage, and sterilization selects that at candlelight carries out, till after nursery stock all takes root. Preferably, the disinfectant of the potassium permanganate solution adopting concentration to be 5% every 57 days after cuttage 1 time. Under the environment of intensity of illumination 2500 3000lx, liquid manure is enriched in 40 60 days after cuttage.
Embodiment 1 Acer palmatum gold thread nursery stock takes root controlled trial
For cottage propagation Acer palmatum gold thread nursery stock, respectively above-described embodiment is tested, bred the Acer palmatum gold thread nursery stock obtained after cuttage rooting, when same, all adopt same mode to manage, managed in conventional liquid manure mode until after cuttage about 50 days start seedling exercising. Seedling exercising is upper pot transplanting after about 20 days, and the cycle data list 1 of taking root measuring Acer palmatum gold thread nursery stock is as follows:
Table 1 Acer palmatum gold thread nursery stock takes root cycle experimental
| Experimental subject | Matched group 1 | Experimental group 1 |
| Take root the cycle (my god) | 180-200 | 50-70 |
Embodiment 2 Acer palmatum gold thread plant percent controlled trial
For cottage propagation Acer palmatum gold thread nursery stock, respectively above-described embodiment is tested, bred the Acer palmatum gold thread nursery stock obtained after cuttage rooting, when same, all adopt same mode to manage, manage in conventional liquid manure mode.The survival rate that after cuttage, 70 days measure gained Acer palmatum gold thread nursery stock, the data obtained list 2 is as follows:
Table 2 Acer palmatum gold thread plant percent is tested
| Experimental subject | Matched group 1 | Experimental group 1 |
| Survival rate | 41.2% 68.6% | More than 90.3% |
The specific embodiment of the present invention is as follows:
Detailed description of the invention 1: described cutting medium is the mixture being made up of the Vermiculitum that volume ratio is 1:2 and perlite; Described process solution is the process solution of (50-150) mg/LNAA+ (250-350) mg/LIBA; Described cutting acquisition time is the 3-4 month.
Detailed description of the invention 2: described cutting medium is the mixture being made up of the Vermiculitum that volume ratio is 1:2 and perlite; Described process solution is the process solution of (20-40) mg/LNAA+ (200-250) mg/LIBA; Described cutting acquisition time be May the first tenday period of a month-mid-August.
Detailed description of the invention 3: described cutting medium is the mixture being made up of the Vermiculitum that volume ratio is 1:2 and perlite; Described process solution is the process solution of (50-150) mg/LNAA+ (250-350) mg/LIBA; Described cutting acquisition time be May the first tenday period of a month-mid-August.
Detailed description of the invention 4: described cutting medium is the mixture being made up of the Vermiculitum that volume ratio is 2:1 and perlite; Described process solution is the process solution of (50-150) mg/LNAA+ (250-350) mg/LIBA; Described cutting acquisition time be May the first tenday period of a month-mid-August.
Detailed description of the invention 5: described cutting medium is the mixture being made up of the Vermiculitum that volume ratio is 1:2 and perlite; Described process solution is that (50-150) mg/LNAA+ (250-350) mg/LIBA processes solution; Described cutting acquisition time is the 9-10 month.
Detailed description of the invention 6: described cutting medium is the mixture being made up of the Vermiculitum that volume ratio is 1:2 and perlite; Described process solution is that 100mg/LNAA+300mg/LIBA processes solution; Described cutting acquisition time be May the first tenday period of a month-mid-August.
Same to breed Acer palmatum gold thread nursery stock, adopt above-mentioned detailed description of the invention 1-6 to carry out cuttage. Aforementioned same mode is adopted to go management cuttage, the data that after cuttage, detection in 70 days is same, the data obtained list 3 is as follows:
Table 3 specific embodiment of the invention
| Detailed description of the invention | 1 | 2 | 3 | 4 | 5 | 6 |
| Take root the cycle (my god) | 50-60 | 60-70 | 55-60 | 60-70 | 50-60 | 50-55 |
| Survival rate (%) | 70.3%-78.4% | 64.2-76.1 | 74.6-80.2 | 84.2-87.6 | 78.9-82.6 | More than 90 |
From table 1, table 2 it can be seen that matched group the Acer palmatum gold thread nursery stock cycle of taking root be 18 200 days, survival rate is 41.2% 68.6%; In experimental group, the Acer palmatum gold thread nursery stock cycle of taking root is 50 70 days, and survival rate is more than 90%, substantially reduces rootage duration, and improves survival rate, improves the breeding cycle.
As can be seen from Table 3: adopt detailed description of the invention 6 i.e. best mode for carrying out the invention to be applied to cuttage Acer palmatum gold thread nursery stock, substantially reduce rootage duration, and improve survival rate, improve the breeding cycle.
Below being only the preferred embodiment of the present invention, it is noted that above-mentioned preferred implementation is not construed as limitation of the present invention, protection scope of the present invention should be as the criterion with claim limited range. For those skilled in the art, without departing from the spirit and scope of the present invention, it is also possible to make some improvements and modifications, these improvements and modifications also should be regarded as protection scope of the present invention.
Claims (10)
1. the method for an Acer palmatum gold thread softwood cutting propagation, it is characterised in that comprise the following steps:
A, using Vermiculitum, perlite as cutting medium, described cutting medium is placed in cuttage seedbed;And to described cutting medium disinfection;
B, on scion-seed tree, gather raw semi-lignified branch then, described semi-lignified branch is cut into cutting, as cuttage fringe bar, and to described cutting disinfection; Cutting base portion after sterilization is soaked 11-13h in the process solution of (50-150) mg/LNAA+ (250-350) mg/LIBA;
C, the cutting cuttage obtained by described step B are to described cuttage seedbed;
D, the cuttage seedbed after step C cuttage is placed under first condition and every 57 days, described cuttage seedbed is carried out disinfection; Described first condition is relative air humidity 80% 95%, air themperature 25 30 DEG C, intensity of illumination 3000 5000lx.
2. the method for Acer palmatum gold thread softwood cutting propagation according to claim 1, it is characterised in that the formula material volume ratio proportioning of described cutting medium: Vermiculitum: perlite is 1:2, mixes standby.
3. the method for Acer palmatum gold thread softwood cutting propagation according to claim 1, it is characterised in that described step A concentration is that the potassium permanganate solution of 5% is to substrate disinfection.
4. the method for Acer palmatum gold thread softwood cutting propagation according to claim 1, it is characterised in that gathering cutting in described step B in the annual mid-August the first tenday period of a month in May, the diameter of the described fringe bar of collection is 0.3cm-0.5cm.
5. the method for Acer palmatum gold thread softwood cutting propagation according to claim 1, it is characterised in that in described step B, semi-lignified branch is cut into cutting method particularly includes:
Described semi-lignified branch cutting into the cutting of 8cm 12cm, removes taper children's tender part during shearing, the upper clip of cutting is horizontal section, lower clip is angle of inclination is the short inclined-plane of 30 45 ��, lower clip distance joint 1 1.5cm, cutting upper end retains 23 leaves, cuts the blade of lower end.
6. the method for Acer palmatum gold thread softwood cutting propagation according to claim 1, it is characterized in that, cutting is carried out disinfection by described step B method particularly includes: described cutting is soaked 10 15min in 700 1200 times of solution of the carbendazim that effective ingredient is 80%.
7. the method for Acer palmatum gold thread softwood cutting propagation according to claim 6, it is characterized in that, cutting is carried out disinfection by described step B method particularly includes: 10 15min are soaked in the two ends that described cutting has wound in 800 1000 times of solution of the carbendazim that effective ingredient is 80%.
8. the method for Acer palmatum gold thread softwood cutting propagation according to claim 1, it is characterised in that the process solution of described step B is: the solution of 100mg/LNAA+300mg/LIBA.
9. the method for Acer palmatum gold thread softwood cutting propagation according to claim 1, it is characterised in that by cutting cuttage to the cuttage seedbed getting out substrate in described step C method particularly includes:
During cuttage, it is not overlapping that cutting density is that described cutting blade connects, and the cuttage degree of depth is the 1/3 1/2 of whole described cutting, by the cutting medium compacting around described cutting after cuttage, water immediately 1 time permeable, make described cutting and cutting medium combine closely simultaneously;
Wherein, described cutting density is spacing in the rows 4cm 5cm, and line-spacing is 4cm 6cm; The described cuttage degree of depth is 4cm 6cm.
10. the method for Acer palmatum gold thread softwood cutting propagation according to claim 1, it is characterised in that described step D is by described step C cuttage post processing method particularly includes:
Described cuttage seedbed is carried out disinfection 1 time by the potassium permanganate solution adopting concentration to be 5% every 57 days after cuttage;Described cuttage seedbed is made to be maintained under the environment of intensity of illumination 2500 3000lx and water after cuttage in 40 60 days.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106171366A (en) * | 2016-06-28 | 2016-12-07 | 广西南宁十分园生态农业科技有限公司 | A kind of hair cuttage rapid propagating method |
| CN106922538A (en) * | 2017-04-26 | 2017-07-07 | 中国科学院合肥物质科学研究院 | A kind of method of Acer saccharum Regeneration in Vitro |
| CN112166847A (en) * | 2020-10-14 | 2021-01-05 | 四川七彩林科股份有限公司 | Efficient propagation method of snow fruits |
| CN112219577A (en) * | 2020-10-26 | 2021-01-15 | 四川七彩林科股份有限公司 | Cutting propagation method of wire netting |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106171366A (en) * | 2016-06-28 | 2016-12-07 | 广西南宁十分园生态农业科技有限公司 | A kind of hair cuttage rapid propagating method |
| CN106922538A (en) * | 2017-04-26 | 2017-07-07 | 中国科学院合肥物质科学研究院 | A kind of method of Acer saccharum Regeneration in Vitro |
| CN106922538B (en) * | 2017-04-26 | 2018-11-23 | 中国科学院合肥物质科学研究院 | A kind of method of Acer saccharum Regeneration in Vitro |
| CN112166847A (en) * | 2020-10-14 | 2021-01-05 | 四川七彩林科股份有限公司 | Efficient propagation method of snow fruits |
| CN112219577A (en) * | 2020-10-26 | 2021-01-15 | 四川七彩林科股份有限公司 | Cutting propagation method of wire netting |
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