CN105632875B - Desorption ionization mass spectrometer interface for mass spectrographic imaging - Google Patents
Desorption ionization mass spectrometer interface for mass spectrographic imaging Download PDFInfo
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- CN105632875B CN105632875B CN201610056147.1A CN201610056147A CN105632875B CN 105632875 B CN105632875 B CN 105632875B CN 201610056147 A CN201610056147 A CN 201610056147A CN 105632875 B CN105632875 B CN 105632875B
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- ion source
- pipeline
- ion
- sample port
- transmission channel
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/26—Mass spectrometers or separator tubes
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/02—Details
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- Analytical Chemistry (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
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Abstract
The invention discloses a kind of desorption ionization mass spectrometer interfaces for mass spectrographic imaging, including ontology, ontology has a funnelform sample port, there is ion source channels and ion transmission channel in ontology, the first end of ion source channels with ion source for connecting, for the first end of ion transmission channel for being connect with mass spectrum, the second end of ion source channels and the second end of ion transmission channel are intersected in sample port.The second end of ion source channels of the present invention and the second end of ion transmission channel are intersected in sample port, this structure type, so that desorption ionization mass spectrum is switched to semi-enclosed mass spectrum by open type mass spectrum, reduce the pollution to sample around, imaging resolution and result accuracy can be effectively improved.
Description
Technical field
The present invention relates to mass spectrometer interface technical fields, and in particular to is used for the desorption ionization mass spectrometer interface of mass spectrographic imaging.
Background technology
Mass spectrograph is a kind of fast, high sensitivity with analyze speed and the scientific analysis instrument with molecular specificity, extensively
It is general to be applied to the various fields such as chemistry, biology, environmental monitoring, health care.And mass spectrum imaging technology is by means of mass spectrographic side
Method analyzes the standard molecule of biomolecule using one under mating mass spectrum imaging software control by measuring mass-to-charge ratio
The mass spectrograph of amount is come the method that is imaged.There are two types of mass spectrum imaging commercial apparatus more mature at present：Secondary Ion Mass Spectrometry and base
Matter assisted laser desorption.And normal pressure open type ionization techniques be since it can carry out ionization process in open environment,
Without sample pre-treatments, can be combined with overwhelming majority commercialization mass spectrograph, ionization mode is soft, generates the advantages that fragment is few, close several
Year has obtained many concerns.
Wherein, desorption electrospray ionization is a kind of side of current normal pressure open type ionization massspectrum imaging field most study
Method.Its principle is：Under the drive of atomization gas, solvent forms electron spray under high pressure, and purges sample surfaces at a certain angle；
During the molecule contacts with sample surfaces, by part analyte molecular melting, and secondary charged drop beam is formed；It is secondary
Grade charged drop beam sprays into mass spectrum entrance at an appropriate angle, and then is detected and analyzed.Since desorption electrospray ionizes base
Originally test substance can be detected by not needing sample pre-treatments or additional Matrix-assisted, thus can use it for sample
The in situ detection of product.
But so far, there are still some shortcomings for desorption electrospray ionization：
One, the electron spray sprayed in sprayer can it is larger due to the internal diameter of sprayer and cause its mass spectrum imaging resolution ratio compared with
It is low；
Two, existing desorption electrospray ionization interface is open type, therefore the diffusion sprayed can cause except sample
Part be contaminated, influence mass spectrum imaging, make imaging repeatability reduce；
Three, part of spray can be adsorbed on sample surfaces, be not easy to remove, and also influence whether ionization and imaging effect.
Invention content
The present invention is in view of the above-mentioned problems, overcome at least one deficiency, it is proposed that a kind of desorption electricity for mass spectrographic imaging
From mass spectrometer interface.
The technical solution adopted by the present invention is as follows：
A kind of desorption ionization mass spectrometer interface for mass spectrographic imaging, including ontology, the ontology are funnelform with one
Sample port, ontology is interior to have ion source channels and ion transmission channel, and the first end of the ion source channels is used for and ion
Source connects, and for the first end of ion transmission channel for being connect with mass spectrum, second end and the ion transmission of the ion source channels are logical
The second end in road is intersected in the sample port.
The second end of ion source channels and the second end of ion transmission channel are intersected in sample port, this structure type,
So that maldi ion ionization mass spectrometry is switched to semi-enclosed mass spectrum by open type mass spectrum, reduces the pollution to sample around so that at
As result is more accurate.Funnelform sample port can prevent spraying from spreading, and effectively avoid the pollution to external sample.
Further, the minimum diameter of the sample port ranging from 10 μm~100mm.
It can be made a change for different resolution demand when practice.
Further, the cross-sectional area of the ion source channels and ion transmission channel at sample port gradually becomes
Change, and closer to sample port, cross-sectional area is smaller.
The variation of ion source channels and this cross-sectional area of ion transmission channel, enables to spread of spray to reduce,
Realize the imaging of higher resolution.
Further, the sample port is connect by exhaust tube with vacuum pump.
The effect of vacuum pump be so that sample port be low pressure, avoid the reagent entered from ion source channels from being adsorbed on sample
Product surface.
Further, the sample port has the mounting hole for installing the exhaust tube.
Further, the ontology includes Y tube and funnelform sampling member, and Y tube includes the first pipeline, the second pipe
Road and third pipeline, wherein the first pipeline of third piping connection and the second pipeline have partition board, third pipeline in third pipeline
Cross section gradually change, and closer to sample port, the cross-sectional area of third pipeline is smaller；The partition board is by Y tube
Inner space is separated into two parts, respectively ion source channels and ion transmission channel.
The ion that ion source can be inhibited to generate by partition board is directly entered mass spectrum.
Sampling member is detachably arranged at the port of third pipe, can replace various sizes of sampling member as needed,
When practice, sampling member can also be integrally formed with Y tube, constitute a single piece.
Further, the interface connect for sample ionization and with mass spectrum use.
Further, the ion source is electric spray ion source, atmospheric pressure chemical ionization ion source, plasma class ion
Source or desorption ionization ion source.
The beneficial effects of the invention are as follows：The second end of ion source channels and the second end of ion transmission channel are intersected in sampling
Port, this structure type make desorption ionization mass spectrum switch to semi-enclosed mass spectrum, funnelform sample port by open type mass spectrum
Sampling area can be reduced, while reducing the pollution to sample around, imaging resolution can be effectively improved and result is accurate
Property.
Description of the drawings：
Fig. 1 is structural schematic diagram of the present invention for the desorption ionization mass spectrometer interface of mass spectrographic imaging；
Fig. 2 is sectional view of the present invention for the desorption ionization mass spectrometer interface of mass spectrographic imaging；
Fig. 3 is operating diagram of the present invention；
Fig. 4 is the enlarged drawing at A in Fig. 3.
Each reference numeral is in figure：
1, Y tube, the 2, first pipeline, the 3, second pipeline, 4, third pipeline, 5, ion source channels, 6, ion transmission channel,
7, partition board, 8, sampling member, 9, exhaust tube, 10, vacuum pump, 11, analysis target, 12, ion, 13, ion source, 14, mass spectrum takes over.
Specific implementation mode：
With reference to each attached drawing, the present invention is described in detail.
As shown in Fig. 2, a kind of desorption ionization mass spectrometer interface for mass spectrographic imaging, including ontology, ontology have a leakage
Bucket-shaped sample port, has ion source channels 5 and ion transmission channel 6 in ontology, the first ends of ion source channels 5 be used for
Ion source connects, and the first end of ion transmission channel 6 with mass spectrum for connecting, second end and the ion transmission of ion source channels 5
The second end in channel 6 is intersected in sample port.
Ion source channels 5 and ion transmit cross-sectional area of 6 channels at sample port and gradually change, and closer to
Sample port, cross-sectional area are smaller.The variation of ion source channels and this cross-sectional area of ion transmission channel, can make
It obtains sample range to reduce, realizes the imaging of higher resolution.
The minimum diameter of sample port ranging from 10 μm~100mm, when practice, can do for different resolution demand
Go out to change.
As shown in Figures 3 and 4, when practice, sample port is connect by exhaust tube 9 with vacuum pump 10, sample port tool
There is the mounting hole of installation exhaust tube.The effect of vacuum pump be so that sample port be low pressure, avoid ion source channels enter
Reagent is adsorbed on sample surfaces, influences test sample process.
As illustrated in fig. 1 and 2, in this present embodiment, ontology includes Y tube 1 and funnelform sampling member 8, and Y tube 1 wraps
Including the first pipeline 2, the second pipeline 3 and third pipeline 4, wherein third pipeline 4 connects the first pipeline 2 and the second pipeline 3, the
There are partition board 7, the cross section of third pipeline 4 to gradually change in three pipelines 4, and closer to sample port, the cross section of third pipeline 4
Area is smaller；The inner space of Y tube is separated into two parts, respectively ion source channels 5 and ion transmission channel 6 by partition board 7.
The ion that ion source can be inhibited to generate by partition board is directly entered mass spectrum.
The miner diameter end of sampling member 8 is mounted on the port of third pipeline 4, and sampling member and the port of third pipeline constitute sampling end
Mouthful.Sampling member is detachably arranged at the port of third pipe, various sizes of sampling member can be replaced as needed, in reality
With when, sampling member can also be integrally formed with Y tube, constitute a single piece.
The interface of the present embodiment is for sample ionization and use is connect with mass spectrum.
Ion source in the present embodiment is electric spray ion source, atmospheric pressure chemical ionization ion source, plasma class ion
Source or desorption ionization ion source.
As shown in Figures 3 and 4, sampling member alignment analysis target 11 when the present embodiment practice, the first pipe and ion source 13
Connection, for receive ion source generation ion 12, ion 12 by after ion source channels 5 and sample port with analysis target 11
On sample contact, during the molecule contacts with sample surfaces, sample molecule is ionized；Sample molecule after ionization
Enter ion transmission channel 6 by sample port, and mass spectrum is entered by mass spectrum take over 14.
The second end of the ion source channels of the present embodiment and the second end of ion transmission channel are intersected in sample port, this
Structure type makes maldi ion ionization mass spectrometry switch to semi-enclosed mass spectrum by open type mass spectrum, reduces the dirt to sample around
Dye, can effectively improve imaging resolution and result accuracy.
It these are only the preferred embodiment of the present invention, not thereby limit the scope of patent protection of the present invention, every fortune
The equivalent structure transformation made by description of the invention and accompanying drawing content is directly or indirectly used in other relevant technology necks
Domain includes similarly within the scope of the present invention.
Claims (5)
1. a kind of desorption ionization mass spectrometer interface for mass spectrographic imaging, including ontology, which is characterized in that the ontology has one
Funnelform sample port, ontology is interior, and there is ion source channels and ion transmission channel, the first end of the ion source channels to use
Connect in ion source, the first end of ion transmission channel for being connect with mass spectrum, the second end of the ion source channels and from
The second end of sub- transmission channel is intersected in the sample port；
The sample port is connect by exhaust tube with vacuum pump, and the sample port has the installation for installing the exhaust tube
Hole；
The ion source is electric spray ion source, atmospheric pressure chemical ionization ion source, plasma class ion source or desorption electricity
Luxuriant component.
2. being used for the desorption ionization mass spectrometer interface of mass spectrographic imaging as described in claim 1, which is characterized in that the sampling end
The minimum diameter ranging from 10 μm~100mm of mouth.
3. being used for the desorption ionization mass spectrometer interface of mass spectrographic imaging as described in claim 1, which is characterized in that the ion source
The cross-sectional area of channel and ion transmission channel at sample port gradually changes, and closer to sample port, cross section
Area is smaller.
4. being used for the desorption ionization mass spectrometer interface of mass spectrographic imaging as described in claim 1, which is characterized in that the ontology packet
Y tube and funnelform sampling member are included, Y tube includes the first pipeline, the second pipeline and third pipeline, wherein third pipe
Road connects the first pipeline and the second pipeline, has partition board, the cross section of third pipeline to gradually change in third pipeline, and closer to adopting
The cross-sectional area of sample port, third pipeline is smaller；The inner space of Y tube is separated into two parts by the partition board, respectively
Ion source channels and ion transmission channel.
5. being used for the desorption ionization mass spectrometer interface of mass spectrographic imaging as claimed in claim 4, which is characterized in that the interface is used
It connect in sample ionization and with mass spectrum use.
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| CN109298063A (en) * | 2018-10-15 | 2019-02-01 | 广东工业大学 | A kind of method of quick detection facial mask natural component |
| CN113764257B (en) * | 2021-08-25 | 2024-01-12 | 清华大学深圳国际研究生院 | Desorption electrospray ionization device based on 3D printing |
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| CN2462385Y (en) * | 2000-09-22 | 2001-11-28 | 中国科学院安徽光学精密机械研究所 | Fast sampling and taking device of mass analyzer |
| CN101201335B (en) * | 2006-12-29 | 2011-06-15 | 东华理工学院 | Surface desorption atmospheric chemical ionization source of mass spectrometer |
| US9595428B2 (en) * | 2014-06-17 | 2017-03-14 | The Board Of Regents Of The University Oklahoma | Cellular probe device, system and analysis method |
| CN205542703U (en) * | 2016-01-27 | 2016-08-31 | 浙江大学 | A desorption ionization mass spectroscopy interface that is used for high -resolution of mass spectrum to form images |
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Effective date of registration: 20160520 Address after: 310027 Hangzhou, Zhejiang Province, Xihu District, Zhejiang Road, No. 38, No. Applicant after: Zhejiang University Address before: 310027 Hangzhou, Zhejiang Province, Xihu District, Zhejiang Road, No. 38, No. Applicant before: Zhejiang University Applicant before: Pan Yuanjiang |
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