CN105628482B - Blood cell analyzer uses the dilution process and device of concentration and dilution liquid - Google Patents
Blood cell analyzer uses the dilution process and device of concentration and dilution liquid Download PDFInfo
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- CN105628482B CN105628482B CN201610059586.8A CN201610059586A CN105628482B CN 105628482 B CN105628482 B CN 105628482B CN 201610059586 A CN201610059586 A CN 201610059586A CN 105628482 B CN105628482 B CN 105628482B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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Abstract
Blood cell analyzer uses the dilution process and device of concentration and dilution liquid, including upper water box (8) through connection solenoid valve (9) connection lower header (11), equipped with through water injecting electromagnetic valve (3) connection pure water making machine (2) on upper water box (8), concentration and dilution liquid packet (13) are connected through peristaltic pump (16), and upper water box level probe (14) and agitating device (22) are housed, equipped with the detection system being made of conductivity sensing electrodes (15) and PH meter probe (19), equipped with by heater (23), temperature protection switch (5), the constant temperature system of temperature sensor (6) composition；Lower part outlet (12) in lower header (11) connects blood cell analyzer dilution import (1) by the road, and lower header liquid level float sensor (10) is housed.The concentration and dilution liquid of inner wrapping can be used at common dilution for instrument in user terminal automatic dilution, greatly save logistics cost, automation dilution also makes user experience unaffected.
Description
Technical field
The present invention relates to blood of human body detecting instrument technical field, especially a kind of blood cell analyzer is dilute using being concentrated
Release the dilution process and device of liquid.
Background technique
Blood cell analyzer belongs to medical precision instrument, for measuring the relevant information of blood cell.In routine use
In, dilution is the main wash agent during Principal diluent and instrument maintenance in its detection process, so dilution
Daily consumption is bigger.At present instrument using when be all directly by instrument pipette insertion drum packaging common dilution bucket in
It uses, it is every to have used one barrel just to replace once.The common common packaging of dilution is every barrel of 20L, will replace one within general 5-6 days
Bucket, thus user often to buy common dilution or once make a big purchase in large quantities, according to the weight and volume of common dilution
It calculates, logistics transportation cost is very high, undoubtedly increases the use cost of user.And the concentration and dilution liquid packaging volume of high concentration
Small, convertible dilution liquid measure is more, and then its transportation cost will greatly reduce.If user has the pure water system of other instruments adapted
Water dispenser, then selection will will be greatly reduced the testing cost of blood cell analyzer using the concentration and dilution liquid of high concentration.
Summary of the invention
The object of the present invention is to provide a kind of blood cell analyzer uses for reducing blood cell analyzer use cost
The dilution process and device of concentration and dilution liquid.
The purpose of the present invention is what is realized by following approach:
Blood cell analyzer uses the dilution device of concentration and dilution liquid, it includes being connected by upper water box 8 through being connected to solenoid valve 9
Lower header 11 is connect, equipped with pure water making machine 2 is connected through water injecting electromagnetic valve 3 on the upper water box 8, connects and is concentrated through peristaltic pump 16
Dilution packet 13, and upper water box level probe 14 and agitating device 22 are housed, equipped with by conductivity sensing electrodes 15 and PH meter
The detection system that probe 19 forms, equipped with the constant temperature system being made of heater 23, temperature protection switch 5, temperature sensor 6；
Blood cell analyzer dilution import 1 is connected by the road in the lower part outlet 12 of the lower header 11, and lower header is housed
Liquid level float sensor 10.
It is further that the peristaltic pump 16 connects on the pipeline of upper water box 8 equipped with detection optocoupler 17.
It is further that upper water box gas outlet 20 and lower header gas outlet 24 are housed on the upper water box 8 and lower header 11,
Connection three-way connection 18 is followed by exhaust outlet by the road.
Be further the agitating device 22 be driven by a motor agitating paddle composition.
It is further the volume of the lower header 11 greater than upper water box 8.
Blood cell analyzer uses the dilution process of concentration and dilution liquid, it includes being connected by upper water box 8 through being connected to solenoid valve 9
Lower header 11 is connect, dilution process is as follows:
A. concentration and dilution liquid is diluted in upper water box 8:
1., when the dilution in lower header 11 uses setting value, liquid level float sensor 10 will in lower header 11
Request signal is issued, peristaltic pump 16 is opened and quantitatively adds in concentration and dilution liquid to upper water box 8, opens simultaneously water injecting electromagnetic valve 3 certainly
In dynamic injection pure water to upper water box 8, level probe 14 closes water injecting electromagnetic valve 3 after detecting signal immediately, opens agitating device
22 are mixed, and when liquid level covers heater 23, constant temperature system is started to work；
2. is when mixing completion, when temperature is heated to set temperature T, the conductivity sensing electrodes 15 of detection system start to examine
The conductivity of dilution is surveyed, PH counts probe 19 and detects dilution pH value, and parameter meets range of set value and then completes concentration and dilution liquid
Dilution, otherwise alarm；
3. after completes dilution, opening the connection solenoid valve 9 of connection lower header 11, the dilution in upper water box 8 being discharged into
Lower header 11 is used for instrument；
B. concentration and dilution liquid packet is needed to change after concentration and dilution liquid packet is finished:
1. just alarms when peristaltic pump 16 connects after the detection optocoupler 17 on 8 pipeline of upper water box detects no concentration and dilution liquid,
The request signal of lower header liquid level sensor 10 is closed, replaces concentration and dilution liquid packet manually；
2. click upper computer software replacement dilution packet function, starting peristaltic pump 16 perfusion concentration and dilution liquid to its pipeline,
After dilution detection sensor detects no dilution in blood cell analyzer, upper water box 8 and lower header 11 are emptied, is opened
Water injecting electromagnetic valve 3 injects pure water rinsing into upper water box 8, then opens connection solenoid valve 9 and pour down the liquid in upper water box 8
Water tank 11 takes the liquid in upper water box 8 and lower header 11 away emptying with the waste drains pump in blood cell analyzer；
3. the dilution in upper water box 8 is discharged into lower header 11 for instrument after diluting concentration and dilution liquid in upper water box 8
It uses.
When being further that the pure water is automatically injected upper water box 8, pure water injection rate is controlled by feed liquor flow resistance, upper water
Rate of release is controlled by flow resistance when dilution in case 8 is discharged into lower header 11, realizes that liquid level steadily rises.
The positive effect of the present invention: scheme proposed by the present invention and its blood cell analyzer use the dilute of concentration and dilution liquid
Method and device is released, the concentration and dilution liquid of inner wrapping can be used at common dilution for instrument in user terminal automatic dilution,
User's common dilution for purchasing ship bulky high-frequency for a long time is saved in this way, logistics cost is greatly saved, automatically
The dilution of change also makes user experience unaffected, is supplied to the common dilution that blood cell analyzer uses by daily and changes
It for concentration and dilution liquid and realizes user terminal automatic dilution, and does not have to change instrument interior structure, that is, reduce user cost again not
User experience is influenced, realizes serial dilution and use with packet concentration and dilution liquid, when without interruption instrument test and avoiding replacement
Dilution is wasted, replaces concentration and dilution liquid packet dilution process, sufficiently and reasonably considering makes under concentration and dilution liquid different situations
With mode, the accuracy of the dilution parameter after dilution can be detected under isoperibol, in ensuring property of concentration and dilution liquid factory
One-time detection is carried out under the premise of energy again, is further ensured that the performance of dilution.
Detailed description of the invention
Fig. 1 is technical solution of the present invention schematic diagram
Fig. 2 is that concentration and dilution liquid of the invention dilutes flow chart
Fig. 3 is that replacement concentration and dilution liquid packet of the invention dilutes flow chart
Fig. 4 is the device of the invention schematic diagram
In figure: 1. blood cell analyzer dilution imports, 2. pure water making machines, 3. water injecting electromagnetic valves, 4. connectors, 5. temperature
Degree protective switch, 6. temperature sensors, 7. dilution water tanks, 8. upper water boxs, 9. connection solenoid valves, 10. liquid level float sensors,
11. lower header, 12. lower header bottom fittings, 13. concentration and dilution liquid packets, 14. upper water box level probes, 15. conductivity detection electricity
Pole, 16. peristaltic pumps, 17. detection optocouplers, 18. three-way connections, 19.PH count probe, 20. upper water box gas outlets, and 21. upper water boxs connect
Head, 22. agitating devices, 23. heaters, 24. lower header gas outlets
Specific embodiment
It elaborates with reference to the accompanying drawings of the specification to the present invention:
To make the purpose of the present invention, technical solution and effect clearer, clear and definite, a kind of device is devised to the present invention
Scheme is further described in detail.It should be appreciated that described device is only one kind that the technical solution is realized, it is not used to
Limit the present invention.
Overall plan realization means of the invention are: designing the dilution that a kind of blood cell analyzer uses concentration and dilution liquid
Device and dilution process make user terminal be mixed well and be completed in a vessel using the quantitative pure water amount of reordering concentration and dilution liquid
Parameter detecting becomes common dilution, resupplies blood cell analyzer use, as shown in Figure 1.
The blood cell analyzer that the present invention program is related to uses the dilution process and device of concentration and dilution liquid, and device is such as
Shown in Fig. 4, comprising: blood cell analyzer dilution import 1, pure water making machine 2, water injecting electromagnetic valve 3, temperature protection switch 5,
Temperature sensor 6, dilution water tank 7 wherein include upper water box 8 and lower header 11, are connected to solenoid valve 9, lower header liquid level float sensing
Device 10, concentration and dilution liquid packet 13, upper water box level probe 14, conductivity sensing electrodes 15, peristaltic pump 16 detect optocoupler 17, PH
Count probe 19, agitating device 22, heater 23, connector and pipeline.Blood cell analyzer dilution import 1 is by pipeline under
Water tank bottom connector 12 is connected to, and pure water making machine 2 is connect by pipeline with water injecting electromagnetic valve 3, so with upper water box top contact 4
Connection, when water injecting electromagnetic valve 3 is opened, pure water is injected into upper water box 8 by pure water by the pressure in pure water making machine 2；?
Upper water box top surface is embedded with a upper water box level probe 14, when pure water injection liquid level reaches 14 check bit of upper water box level probe
When setting, water injecting electromagnetic valve 3 is automatically closed, realizes that pure water quantitatively injects；Concentration and dilution liquid packet 13 passes through pipeline and upper water box top
Connector 21 is connected to, and a peristaltic pump 16 and a detection optocoupler 17 around the home on its connecting pipeline, detects optocoupler 17 close
21 end of water tank connector, for detecting with or without concentration and dilution liquid, peristaltic pump 16 is used to quantitatively add concentration and dilution liquid；Upper water box 8
Top is also embedded with an agitating device 22, and a motor drives agitating paddle, to mix pure water and concentration and dilution liquid；Upper water box
Top is also embedded with one group of conductivity sensing electrodes 15, the conductivity of detection mixed liquor after mixing sufficiently；One PH counts probe
19, the pH value of detection mixed liquor after mixing sufficiently；Upper water box top surface is additionally provided with a gas outlet 20 and is connected to atmosphere.In upper water box
8 sides constitute thermostatic control system embedded with a heater 23, a temperature sensor 6, a temperature protection switch 5, three,
In 23 heating process of heater, just stop heating immediately when temperature sensor 6 detects set temperature T, if temperature
It is out of control just to cut off heating power supply when reaching temperature set by temperature protection switch 5 immediately and alarm.In upper water box 8 and lower header
A connection solenoid valve 9 is arranged between 11 to be connected to upper lower header.A liquid level float sensor is embedded at the top of lower header 11
10, and be equipped with a connector 24 and be connected to the open to atmosphere connector 20 of upper water box and three-way connection 18.When upper water box dilution mixes
And after detecting well, while after 10 trigger signal of lower header liquid level float sensor, system automatically opens connection solenoid valve 9 will be upper
The dilution of water tank 8 is put into lower header 11.11 side bottom of lower header have a connector 12 and blood cell analyzer dilution into
Liquid mouth 1 is connected to, and blood cell analyzer proportioning device directly draws dilution from lower header.11 volume of lower header is greater than water
Case 8；Upper water box level probe 14 be arranged status requirement be: its alarm when, the water injection rate of upper water box 8 just with the concentration of addition
Dilution is at agreement proportionate relationship；The status requirement that the liquid level float sensor 10 of lower header 11 is arranged is: it is lauched when alarming
The dilution liquid measure of the case 11 enough time that a concentration and dilution liquid is diluted to upper water box 8, to realize same packet concentration and dilution liquid
Continuous use.Heater 23 is to carry out being heated to set temperature T, temperature sensor 6 to 8 liquid of upper water box after pure water is added
It is the temperature in real-time monitoring upper water box 8, heater 23 is allowed to stop heating when the set temperature is reached.The device can be external in
Outside blood cell analyzer, it can also be inscribed in inside blood cell analyzer and use.
Blood cell analyzer uses the dilution process of concentration and dilution liquid, and the dilution process is on device as described above
It carries out, specifically includes following content:
Dilution process in same packet concentration and dilution liquid.As shown in Figure 2
Because dilution water tank will not be made very big, same packet concentration and dilution liquid will will dilute can just be finished several times.Its
Steps are as follows:
A. request upper water box dilution: set amount is upper water box dilution one after the dilution of lower header 11 is only left set amount
Analyzer used diluent amount in the secondary time, lower header liquid level float sensor 10 is with regard to trigger request signal, then upper water box
Start mixed diluting liquid.
B. upper water box dilutes concentration and dilution liquid: after the request for receiving lower header liquid level float sensor 10, peristaltic pump 16 is opened
Quantitative addition concentration and dilution liquid is opened into upper water box 8,17 real-time monitoring of optocoupler on access whether there is or not, once discovery concentration and dilution
Liquid terminates operation without just alarm.Water injecting electromagnetic valve 3 is opened simultaneously, and pure water is automatically injected upper water box 8, and pure water injection rate passes through
The control of feed liquor flow resistance realizes that liquid level steadily rises, and closes first liang immediately after upper water box level probe 14 detects signal and leads to
Solenoid valve 3, to realize that matching for dilution ratio is fixed.Heater is started to work when liquid level covers heater 23 simultaneously.
C. it stirs and evenly mixs: after the completion of dilution proportion, opening agitating device 22 and mixed.
D. it parameter detecting: is completed and when temperature is heated to set temperature allowable error ± 1 DEG C T when mixing, conductivity
Detecting electrode 15 starts the conductivity of the dilution after detection mixes, while PH meter probe 19 detects the dilution after mixing
PH value.Only all meet this time of range of set value dilution there are two parameter and be just counted as function, otherwise alarms.
E. it transfers dilution: after the dilution whole ratio detection of upper water box is completed and met the requirements, opening connection electricity
The dilution of upper water box is discharged into lower header and used for instrument by magnet valve 9, and rate of release avoids lower header from rising by flow resistance control
Bubble.
Replace the dilution process of concentration and dilution liquid packet.As shown in Figure 3
Because difference packet concentration and dilution liquid it is variant between validity period and batch its replacement when dilution process
It is different.Its step are as follows:
A. concentration and dilution liquid packet is replaced in request: when the optocoupler 17 on 13 access of concentration and dilution liquid packet detects no concentration and dilution
Warning note replaces concentration and dilution liquid packet manually after liquid, while the dilution of lower header continues to use, and shields lower header
The request of liquid level sensor 10.
B. concentration and dilution perfusion: manually after replacement concentration and dilution liquid packet, clicking and replace dilution packet function on software, compacted
Dynamic 16 rotation of pump concentration and dilution liquid access is perfused full.
C. upper lower header is rinsed in request: when dilution sensor detects no dilution in blood cell analyzer machine,
Just opening water injecting electromagnetic valve 3 is that water purification machine feed water valve fills the water flushing into upper water box 8, then opens connection solenoid valve 9, by upper water box
8 liquid pours lower header 11, then takes the liquid of upper lower header away emptying using the waste drains pump in blood cell analyzer.
Lower header is repeatedly in pure water rinsing and emptying can clean up.
D. upper water box dilutes concentration and dilution liquid: after upper lower header clean-drain, shutting connection solenoid valve 9；Peristaltic pump
16 open quantitatively addition concentration and dilution liquid into upper water box 8,17 real-time monitoring of optocoupler on access whether there is or not, once discovery concentration
Dilution terminates operation without just alarm.Water injecting electromagnetic valve 3 is opened simultaneously, and pure water is automatically injected upper water box 8, pure water injection rate
It is controlled by feed liquor flow resistance, realizes that liquid level steadily rises, close water filling immediately after upper water box level probe 14 detects signal
Solenoid valve 3, to realize that matching for dilution ratio is fixed.Heater is started to work when liquid level covers heater 23 simultaneously.
E. it stirs and evenly mixs: after the completion of dilution proportion, opening agitating device 22 and mixed.
F. it parameter detecting: is completed and when temperature is heated to set temperature allowable error ± 1 DEG C when mixing, conductivity inspection
The conductivity that electrode 15 starts the dilution after detection mixes is surveyed, while PH meter probe 19 detects the dilution PH after mixing
Value.Only all meet this time of range of set value dilution there are two parameter and be just counted as function, otherwise alarms.
G. it transfers dilution: after the completion of the dilution whole ratio detection of upper water box 8, opening second liang of three-way electromagnetic valve 9
The dilution of upper water box 8 is discharged into lower header 11 to be perfused and use for instrument, rate of release avoids being lauched by flow resistance control
Case blistering.
The present invention program proposes to be supplied to the common dilution that blood cell analyzer uses by daily and be changed to concentration and dilution
Liquid simultaneously realizes user terminal automatic dilution, and does not have to change instrument interior structure, that is, reduce user cost and do not influence user's body
It tests.The device of the invention will dilute water tank and be divided into two parts water tank up and down, supplies and dilutes to blood cell analyzer in lower header
While liquid upper water box can carry out in advance concentration and dilution liquid dilution movement, thus realize with packet concentration and dilution liquid serial dilution and
It uses, without interruption instrument test and avoids wasting dilution when replacement.Heater, temperature sensor are provided on upper water box
And temperature protection switch, there are also conductivity sensing electrodes and PH to count electrode, and former three constitutes constant temperature system, and rear the two can be in perseverance
The accuracy of dilution parameter under warm environment after detection dilution carries out again under the premise of the factory of concentration and dilution liquid ensures performance
One-time detection is further ensured that the performance of dilution.It is provided with liquid level detection electrode on upper water box and guarantees that liquid total amount is matched in dilution,
Concentration and dilution liquid peristaltic pump quantitative filling simultaneously, so that it is guaranteed that the proportion relation of concentration and dilution liquid and pure water.On upper water box
It may insure that concentration and dilution liquid is mixed well with pure water embedded with an evenly mixing device.In the access of concentration and dilution liquid packet and upper water box
It above can be with the presence or absence of real-time detection concentration and dilution liquid equipped with detection optocoupler.In lower header top surface embedded with a liquid level float sensing
Device, when lower header dilution less than it is a certain amount of when automatic trigger request upper water box be diluted liquid proportion, to mend in time
Fill the dilution liquid measure of lower header, and then uninterrupted instrument test." the dilution in same packet concentration and dilution liquid that the present invention program proposes
Process " and " replacement concentration and dilution liquid packet dilutes process ", sufficiently and reasonably consider the use under concentration and dilution liquid different situations
Mode is compared with common mode does not influence instrument test and user experience.
Claims (2)
1. a kind of blood cell analyzer uses the dilution process of concentration and dilution liquid, it includes by upper water box (8) through being connected to electromagnetism
Valve (9) connects lower header (11), it is characterized in that dilution process is as follows:
A. concentration and dilution liquid is diluted in upper water box (8):
1., when the dilution in lower header (11) uses setting value, the interior liquid level float sensor (10) of lower header (11) is just
Request signal can be issued, peristaltic pump (16) is opened and quantitatively adds in concentration and dilution liquid to upper water box (8), open simultaneously water filling electromagnetism
Valve (3) is automatically injected in pure water to upper water box (8), and level probe (14) closes water injecting electromagnetic valve (3) after detecting signal immediately,
It opens agitating device (22) to be mixed, when liquid level covers heater (23), constant temperature system is started to work；
2. is when mixing completion, when temperature is heated to set temperature T, the conductivity sensing electrodes (15) of detection system start to detect
The conductivity of dilution, PH count probe (19) and detect dilution pH value, and parameter meets range of set value and then completes concentration and dilution liquid
Dilution, otherwise alarm；
3. after completes dilution, opening the connection solenoid valve (9) of connection lower header (11), the dilution in upper water box (8) is discharged
It is used to lower header (11) for instrument；
B. concentration and dilution liquid packet is needed to change after concentration and dilution liquid packet is finished:
1. is just reported after the detection optocoupler (17) on peristaltic pump (16) connection upper water box (8) pipeline detects no concentration and dilution liquid
It is alert, the request signal of lower header liquid level sensor (10) is closed, replaces concentration and dilution liquid packet manually；
2. click upper computer software replacement dilution packet function, starting peristaltic pump (16) perfusion concentration and dilution liquid to its pipeline, when
After dilution detection sensor detects no dilution in blood cell analyzer, upper water box (8) and lower header (11) are emptied, is beaten
Water injecting electromagnetic valve (3) are opened, pure water rinsing is injected into upper water box (8), then opening connection solenoid valve (9) will be in upper water box (8)
Liquid pours lower header (11), with the waste drains pump in blood cell analyzer by the liquid in upper water box (8) and lower header (11)
Take emptying away；
3. the dilution in upper water box (8) is discharged into lower header (11) for instrument after dilution concentration and dilution liquid in upper water box (8)
Device uses.
2. dilution process according to claim 1, it is characterised in that when the pure water is automatically injected upper water box (8), pure water
Injection rate is controlled by feed liquor flow resistance, and rate of release passes through stream when the dilution in upper water box (8) is discharged into lower header (11)
Resistance control realizes that liquid level steadily rises.
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| CN201610059586.8A CN105628482B (en) | 2016-01-28 | 2016-01-28 | Blood cell analyzer uses the dilution process and device of concentration and dilution liquid |
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| CN201610059586.8A CN105628482B (en) | 2016-01-28 | 2016-01-28 | Blood cell analyzer uses the dilution process and device of concentration and dilution liquid |
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| CN105628482B true CN105628482B (en) | 2019-04-05 |
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| JP6285998B1 (en) * | 2016-08-31 | 2018-02-28 | シスメックス株式会社 | Smear preparation apparatus and smear preparation method |
| CN106582377A (en) * | 2016-12-28 | 2017-04-26 | 天津通缘和泰科技发展有限公司 | Innovative blood purifier |
| CN106950096A (en) * | 2017-03-31 | 2017-07-14 | 天津中科先进技术研究院有限公司 | Lithium cell is anodal with combined material testing arrangement |
| CN106840833B (en) * | 2017-04-21 | 2019-09-03 | 青岛市中心血站（青岛市公民义务献血办公室青岛市输血医学研究所） | A kind of blood mixing auxiliary device |
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| EP3714252B1 (en) * | 2017-11-22 | 2023-05-31 | Beckman Coulter, Inc. | Diluent preparation module und method for supplying such diluent |
| CN108267356A (en) * | 2018-02-08 | 2018-07-10 | 中国科学院寒区旱区环境与工程研究所 | A kind of sample automatic dilution device |
| CN108318608A (en) * | 2018-02-08 | 2018-07-24 | 中国科学院寒区旱区环境与工程研究所 | A kind of method of sample automatic dilution |
| CN109085369A (en) * | 2018-08-09 | 2018-12-25 | 苏州长光华医生物医学工程有限公司 | Automatic liquid mixing system and method suitable for chemical illumination immunity analysis instrument |
| CN113310881A (en) * | 2021-05-11 | 2021-08-27 | 中国第一汽车股份有限公司 | Automatic sodium chloride corrosive liquid supplementing equipment and method |
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