CN105624832B - The method that the calcium alginate microfibre of tool bat structure is prepared based on microflow control technique - Google Patents
The method that the calcium alginate microfibre of tool bat structure is prepared based on microflow control technique Download PDFInfo
- Publication number
- CN105624832B CN105624832B CN201410623961.8A CN201410623961A CN105624832B CN 105624832 B CN105624832 B CN 105624832B CN 201410623961 A CN201410623961 A CN 201410623961A CN 105624832 B CN105624832 B CN 105624832B
- Authority
- CN
- China
- Prior art keywords
- sodium alginate
- drop
- fiber
- microfibre
- oil phase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Materials For Medical Uses (AREA)
Abstract
The invention provides a kind of method for the calcium alginate microfibre that tool bat structure is prepared based on microflow control technique.This method produces uniform oil phase drop using drop micro fluidic device first, and in linear array in continuous phase sodium alginate soln；Secondly the sodium alginate soln for including drop is continuously passed through in aqueous isopropanol by chip outlet, sodium alginate solute is separated out, form sodium alginate fiber, fiber is immersed in calcium chloride, by ion sequestration, the internal bat structure calcium alginate microfibre for including single-size oil phase drop is formed.The advantage of the invention is that the drop micro fluidic device can realize the arrangement to " ball " type structure inside above-mentioned calcium alginate microfibre, spacing, the height control of size；It can be achieved to control the height of above-mentioned calcium alginate microfibre " rod " type diameter of movement；The height maintenance and anti-swelling of tool bat structure calcium alginate microfibre can be achieved.
Description
Technical field
The invention belongs to new material technology and the crossing domain of microflow control technique, and in particular to one kind is based on micro-fluidic skill
The method that art prepares the calcium alginate microfibre of tool bat structure.
Background technology
The present invention is using microflow control technique as support.Micro-fluidic chip is that one kind manipulates microbody in minim channel or structure
The system of product flow of fluid, the wherein yardstick of passage and component are tens to hundreds of microns.Each operation of micro-fluidic chip is single
Member is connected each other by the flowing of fluid in microchannel network, and fluid flows in a certain way in the microchannel network, micro-
Fluidic chip can realize specific function on the whole.Microfluidic chip technology is in biological, chemistry, the sample of medical analysis process
It is widely used in terms of product preparation, reaction, separation, detection.In recent years with the hair of material science, micro-nano process technology
Exhibition, microfluidic chip technology also achieves breakthrough in micron order Material Field.It is highly integrated due to micro-fluidic chip
Change and homogeneity so that microsize, pattern are various, the preparation of height homogeneous material structure is achieved, biomedical and
Material Field possesses very big development prospect.
Fiber refers to the material being made up of continuous or discrete filament, and flexible modulus is big, and plastic deformation is small, intensity
High the features such as.The multilevel hierarchy (blood vessel, muscle fibre, nerve tract, hepatic cell cords etc.) of human body is all made up of fibre structure, therefore
Fiber plays an important role maintaining organizational aspects.The preparation method of current fibrous material it is many using spinning and microflow control technique as
It is main.Spinning can prepare submicron order, or even nano-scale fiber, and what polymer solution was extruded through spinning head pore is generally single group
The single-size solid fibre silk divided, forms variously-shaped fibrous material, the fiber formed by adjusting receiver path
Material is by many filament accumulation moldings；It is fine that microflow control technique one-time formed can prepare submicron order of different shapes
Dimension, can prepare porous, hollow and complicated fibre structure, and the preparation of one pack system or multi-component fiber can be achieved.Miniflow
Control technology is occupied an leading position with its Highgrade integration, small but excellent thin platform advantage in the research of fibrous material.
Sodium alginate is similar to the glycosaminoglycan in extracellular matrix, possesses good biocompatibility.Sodium alginate holds
Easily combined with some bivalent cations, form gel, sodium alginate and Ca2+Chelating, can form stable calcium alginate gel.
Due to the collosol and gel property that sodium alginate is gentle so that microflow control technique prepares calcium alginate fibre and is possibly realized.At present, lead to
The preparation of different size calcium alginate fibres can be realized by crossing microflow control technique, and the preparation of hollow calcium alginate fibre can be achieved, can
As release or the carrier of embedding medicinal, albumen and cell, micro-structural in analogue body.Microflow control technique is mutually tied with biomaterial
Close, the preparation of various micro-structurals can be achieved, there is very big development prospect in biomedical and field of tissue engineering technology.
The content of the invention
It is an object of the invention to provide a kind of calcium alginate microfibre that tool bat structure is prepared based on microflow control technique
Method, preparation process of the present invention is stable, and product morphology is homogeneous controllable, simple to operate.
The present invention produces uniform oil phase drop using drop micro fluidic device, and in linear array in continuous phase sea
In solution of sodium alginate.The sodium alginate soln that has oil phase drop will be forgiven continuously to be passed into by the liquid spout of micro fluidic device
In isopropanol, the polarity (3.9) of isopropanol is weaker than water (9.0), in low polar solvent ionic polymer due to ion dipole
The attraction effect of son is easily assembled, and therefore, sodium alginate soln is passed through in aqueous isopropanol, occurs dehydration aggregation, sea
Mosanom solute separates out solidification, forms sodium alginate solid fiber, and show the bat form of series connection；By the alginic acid of solidification
Sodium fiber, which is immersed in, makes sodium alginate chelate the stable calcium alginate fibre of generation with calcium ion in high calcium chloride concentration solution.This
The oil phase drop is invented for water insoluble liquid also insoluble in isopropanol, is passed into sodium alginate soln in isopropanol
Afterwards, oil phase drop is fixed in sodium alginate fiber, and along fiber in linear array.The micro-fluidic dress of drop of the present invention
Oil phase drop can be generated by putting, and by adjusting the spacing between droplet distribution phase and continuous phase flow velocity, control oil phase drop, make each liquid
Fixed range is spaced between drop or makes each drop closely adjacent；Droplet distribution phase flow rates are 0.5ul/min-2ul/min, even
Continuous phase flow rates are 3ul/min-10ul/min；The oil phase of drop micro fluidic device, which disperses facies unit, suitably to be increased and decreased, with
Increase or decrease oil phase dispersed phase species.
The formation of the bat form of series connection of the present invention, is due to that the oil phase drop diameter in sodium alginate soln is smaller
In the diameter of the sodium alginate soln liquid stream sprayed by micro fluidic device liquid spout, when sodium alginate soln is passed through by spout
After into isopropanol, the sodium alginate fiber for separating out solidification there occurs acutely relative to sodium alginate soln liquid stream in diametric(al)
Contraction, the sodium alginate soln of various concentrations, shrinkage degree is different, and concentration is smaller, shrinks more violent；In no oil phase drop
The fiber position of occupy-place, forms thin " rod " form, at the fiber position for having oil phase drop occupy-place, forms thick " ball " form,
" ball " " rod " spacing structure is arranged, and forms the sodium alginate fiber with bat structure.Sodium alginate ball of the invention by formation
Rod fiber is immersed in calcium chloride solution, fiber is changed into calcium alginate fibre, and fibre morphology structure can be made more to stablize,
Prevent when fiber is submerged in water or salting liquid in when, occur fiber swelling set even become solution.
Brief description of the drawings
The micro-fluidic single unit system schematic diagram of Fig. 1 drops.
Fig. 2 drop micro-fluidic chip schematic diagrames.
Wherein 1 micro-fluidic chip, 2 delivery hoses, 3 sodium alginate soln liquid spouts, 4 oil are as drop, and 5 isopropanols are molten
At liquid, 11 sodium alginate soln entrances, 12 oil phase entrances, the outlet of 13 chips, 14 oil phase drop generations.
The principle schematic of Fig. 3 generation tool bat structure calcium alginate microfibres.
Embodiment：
Embodiment 1
Include the preparation of the calcium alginate microfibre of the tool bat structure of single-size drop.
Mineral oil is passed through chip liquid drop dispersed phase entrance, droplet distribution phase and continuous phase flow velocity are respectively set to：
A. the corresponding flow velocity of droplet distribution phase entrance is 0.5ul/min, continuous phase and cross folder circulation road alginic acid
The flow velocity of sodium solution is 3ul/min；
B. the corresponding flow velocity of droplet distribution phase entrance is 1ul/min, continuous phase and cross folder circulation road sodium alginate
The flow velocity of solution is 6ul/min；
C. the corresponding flow velocity of droplet distribution phase entrance is 2ul/min, continuous phase and cross folder circulation road sodium alginate
The flow velocity of solution is 10ul/min；
It is respectively 1% (w/w), 2% (w/w), 4% (w/w) sodium alginate soln to test concentration, and chip is exported
Vertical with the receiving flask bottleneck holding containing aqueous isopropanol, chip exit is immersed under aqueous isopropanol liquid level, outlet width,
Height is all 100um.By syringe pump pushing syringe, make mineral oil by T-shaped passage, form the drop of size uniformity, pass through
Spacing between cross clevis circulation road sodium alginate soln continuous phase flow velocity, control drop is adjusted, the marine alga that has mineral oil will be forgiven
Acid sodium solution is continuously passed into isopropanol by the liquid spout of micro fluidic device, and dehydration clustering phenomena occurs for sodium alginate,
Sodium alginate fiber is formed, fiber is immersed in 4% (w/w) calcium chloride, by ion sequestration, forms internal comprising homogeneous
The bat structure calcium alginate microfibre of the mineral oil drop of size.This experiment finally prepares 9 groups of marine algas by orthogonal test
Sour calcium microfibre.
Embodiment 2
Have cell culture and the assay of bat structure calcium alginate microfibre
Using laboratory designed, designed and 9 groups of tool bat structure calcium alginate microfibres of preparation, configuration is as shown in Figure 3.
9 groups of bat structure calcium alginate microfibres are subjected to Gradient elution using ethanol (20%, 40%, 60%, 80%, 100%) respectively, soaked
Steep in 75% ethanol, ultraviolet sterilization 2h, containing 10% dual anti-PBS solution soaked overnight, surface modification is carried out using NTx
Bat structure calcium alginate microfibre is inoculated with NSCs cells afterwards, and inoculum density is 1 × 106Liquid is changed after individual/mL, 24h cell attachment,
After cell culture 3 days, the related dyeing in terms of cell life or death, apoptosis, cell propagation, differentiation are detected, Image Pro softwares point
Analysis.
Claims (7)
1. a kind of method for the calcium alginate microfibre that tool bat structure is prepared based on microflow control technique, it is characterised in that：
(1) uniform oil phase drop is produced using drop micro fluidic device, and in linear array in continuous phase sodium alginate
In solution；
(2) sodium alginate soln for having oil phase drop will be forgiven and is continuously passed into isopropanol by the liquid spout of micro fluidic device
In, sodium alginate soln is dehydrated, sodium alginate solute separates out solidification, forms sodium alginate solid fiber, and show series connection
Bat form；
(3) the sodium alginate fiber of solidification is immersed in high calcium chloride concentration solution makes sodium alginate chelate generation with calcium ion
Stable calcium alginate fibre；The concentration of the sodium alginate soln is 1%-4% (w/w)；The concentration of the calcium chloride solution
For 4% (w/w).
2. according to described in claim 1 based on microflow control technique prepare have bat structure calcium alginate microfibre method, its
It is characterised by：The oil phase drop is water insoluble hydrophobic liquid also insoluble in isopropanol, is passed into sodium alginate soln
After in isopropanol, oil phase drop is fixed in sodium alginate fiber, and along fiber in linear array.
3. according to described in claim 2 based on microflow control technique prepare have bat structure calcium alginate microfibre method, its
It is fluorinated oil to be characterised by the hydrophobic liquid.
4. according to described in claim 3 based on microflow control technique prepare have bat structure calcium alginate microfibre method, its
It is FC-40 to be characterised by the fluorinated oil.
5. according to described in claim 1 based on microflow control technique prepare have bat structure calcium alginate microfibre method, its
It is characterised by：The drop micro fluidic device can generate oil phase drop, by adjusting droplet distribution phase and continuous phase flow velocity, control
Spacing between oil phase drop processed, interval is fixed or makes each drop closely adjacent between making each drop；Droplet distribution phase flow rates
It is 0.5ul/min-2ul/min, continuous phase flow rates are 3ul/min-10ul/min.
6. in accordance with the method for claim 1, it is characterised in that：The formation of the bat form of the series connection, is due to marine alga
Oil phase drop diameter in acid sodium solution is slightly less than by the straight of the sodium alginate soln liquid stream of micro fluidic device liquid spout ejection
Footpath, after sodium alginate soln is passed into isopropanol by spout, separates out the sodium alginate fiber of solidification relative to alginic acid
Sodium solution liquid stream there occurs violent contraction in diametric(al)；The sodium alginate soln of various concentrations, shrinkage degree is different, concentration
It is smaller, shrink more violent；At the fiber position without oil phase drop occupy-place, thin " rod " form is formd, is accounted for there is oil phase drop
The fiber position of position, forms thick " ball " form, and the arrangement of " ball " " rod " spacing structure forms the sodium alginate with bat structure
Fiber.
7. in accordance with the method for claim 1, it is characterised in that：The sodium alginate bat fiber of formation is immersed in calcium chloride
In solution, fiber is changed into calcium alginate fibre, fibre morphology structure can be made more to stablize, prevented when fiber is immersed in water
In or salting liquid in when, occur fiber swelling set even become solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410623961.8A CN105624832B (en) | 2014-11-07 | 2014-11-07 | The method that the calcium alginate microfibre of tool bat structure is prepared based on microflow control technique |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410623961.8A CN105624832B (en) | 2014-11-07 | 2014-11-07 | The method that the calcium alginate microfibre of tool bat structure is prepared based on microflow control technique |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105624832A CN105624832A (en) | 2016-06-01 |
| CN105624832B true CN105624832B (en) | 2017-09-22 |
Family
ID=56040161
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410623961.8A Active CN105624832B (en) | 2014-11-07 | 2014-11-07 | The method that the calcium alginate microfibre of tool bat structure is prepared based on microflow control technique |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105624832B (en) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017219305A1 (en) * | 2016-06-23 | 2017-12-28 | The University Of Hong Kong | Cavity-microfibers, methods and apparatuses for the fabrication of microfibers, threads, and filaments |
| CN108149334B (en) * | 2016-12-05 | 2021-03-09 | 中国科学院大连化学物理研究所 | Method for preparing microfiber with complex shape based on microfluidic chip and special chip |
| CN109652359A (en) * | 2017-10-12 | 2019-04-19 | 中国科学院大连化学物理研究所 | A kind of preparation method of the cell 3D culture hydrogel microsphere based on aqueous two-phase drop |
| CN108071007B (en) * | 2017-11-08 | 2020-07-17 | 韩金玲 | Method for preparing silver nanowire and graphene-based calcium alginate composite conductive fiber |
| CN108360088B (en) * | 2018-02-28 | 2020-03-03 | 清华大学深圳研究生院 | Method and device for preparing calcium alginate fibers |
| JP7388722B2 (en) * | 2018-08-10 | 2023-11-29 | 国立大学法人 東京大学 | alginate hollow microfiber |
| CN111250009B (en) * | 2018-12-03 | 2022-12-20 | 成都市银隆新能源有限公司 | Method for preparing lithium ion battery material by using microfluidic technology |
| KR102382612B1 (en) * | 2020-02-03 | 2022-04-05 | (주)인벤티지랩 | A microparticle producing system which comprises carrying fluid, and a controlling method thereof |
| CN113355754A (en) * | 2021-07-07 | 2021-09-07 | 中国计量大学 | Method for preparing multiphase fiber based on microfluidic-electrostatic spinning technology |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05209318A (en) * | 1992-01-30 | 1993-08-20 | Teijin Ltd | Calcium alginate fiber having excellent liquid absorbing property and covering material for medical use |
| JP2000239919A (en) * | 1999-02-19 | 2000-09-05 | Yasuo Hatate | Composite fiber containing shirasu balloon |
| CN101555637A (en) * | 2009-05-06 | 2009-10-14 | 东华大学 | Method for preparing alginate microsphere/high polymer composite nanometer fiber by electrostatic spinning |
| CN102974410A (en) * | 2012-11-06 | 2013-03-20 | 中国科学院大连化学物理研究所 | Method and dedicated chip for preparation of micron calcium alginate filament based on microfluidic chip |
-
2014
- 2014-11-07 CN CN201410623961.8A patent/CN105624832B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05209318A (en) * | 1992-01-30 | 1993-08-20 | Teijin Ltd | Calcium alginate fiber having excellent liquid absorbing property and covering material for medical use |
| JP2000239919A (en) * | 1999-02-19 | 2000-09-05 | Yasuo Hatate | Composite fiber containing shirasu balloon |
| CN101555637A (en) * | 2009-05-06 | 2009-10-14 | 东华大学 | Method for preparing alginate microsphere/high polymer composite nanometer fiber by electrostatic spinning |
| CN102974410A (en) * | 2012-11-06 | 2013-03-20 | 中国科学院大连化学物理研究所 | Method and dedicated chip for preparation of micron calcium alginate filament based on microfluidic chip |
Non-Patent Citations (2)
| Title |
|---|
| 海藻酸钙纤维的制备及阻燃性能研究;姜丽萍等;《阻燃材料与技术》;20080815(第04期);14 * |
| 海藻酸钙纤维的阻燃性能;张传杰等;《印染》;20110831(第08期);1-5 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105624832A (en) | 2016-06-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105624832B (en) | The method that the calcium alginate microfibre of tool bat structure is prepared based on microflow control technique | |
| He et al. | Microfluidic fabrication of bio-inspired microfibers with controllable magnetic spindle-knots for 3D assembly and water collection | |
| Fang et al. | Gradient-sized control of tumor spheroids on a single chip | |
| US10928382B2 (en) | Microfluidic device and method for analysis of tumor cell microenvironments | |
| Shao et al. | Responsive inverse opal scaffolds with biomimetic enrichment capability for cell culture | |
| Choi et al. | One-step generation of cell-laden microgels using double emulsion drops with a sacrificial ultra-thin oil shell | |
| CN106215987B (en) | The controllable spinning process of multichannel cocurrent micro-fluid chip and linear multiphase heterojunction structure fiber based on the chip | |
| Konwarh et al. | Silk-microfluidics for advanced biotechnological applications: A progressive review | |
| JP6534380B2 (en) | Device for producing spheroid, recovery method and production method of spheroid | |
| Zhao et al. | A flexible microfluidic strategy to generate grooved microfibers for guiding cell alignment | |
| Angelozzi et al. | Composite ECM–alginate microfibers produced by microfluidics as scaffolds with biomineralization potential | |
| Zhu et al. | Engineering microcapsules to construct vascularized human brain organoids | |
| CN108360088B (en) | Method and device for preparing calcium alginate fibers | |
| CN109195698A (en) | The method and apparatus of single drop, composite droplet and controlled shape (compound) particle or fiber are manufactured in the air | |
| Munaz et al. | A lab-on-a-chip device for investigating the fusion process of olfactory ensheathing cell spheroids | |
| US9957481B2 (en) | Scaffolds and other cell-growth structures using microfluidics to culture biological samples | |
| WO2020066306A1 (en) | Cell structure, method for producing cell structure, method for culturing cells, and micro-flow path | |
| KR20190051096A (en) | Microfluidic chip and preparation method of free-standing porous membrane using the same | |
| Jeong et al. | Microfluidic spinning of grooved microfiber for guided neuronal cell culture using surface tension mediated grooved round channel | |
| CN106479868A (en) | A kind of microdevice based on multistage dialysis scavenger-cell cryoprotective agent | |
| Miyama et al. | A droplet-based microfluidic process to produce yarn-ball-shaped hydrogel microbeads | |
| EP3852821A1 (en) | Self-assembling graphene oxide-protein matrix | |
| KR101856379B1 (en) | Method for seeding cell | |
| Li et al. | Facile fabrication of a 3D electrospun fibrous mat by ice-templating for a tumor spheroid culture | |
| KR102429707B1 (en) | Automatic apparatus for supplying cell culture media with osmosis pump |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |