CN105602717A - Refining method of horse oil - Google Patents
Refining method of horse oil Download PDFInfo
- Publication number
- CN105602717A CN105602717A CN201510995070.XA CN201510995070A CN105602717A CN 105602717 A CN105602717 A CN 105602717A CN 201510995070 A CN201510995070 A CN 201510995070A CN 105602717 A CN105602717 A CN 105602717A
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- CN
- China
- Prior art keywords
- house oil
- house
- oil
- purification
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/001—Refining fats or fatty oils by a combination of two or more of the means hereafter
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/006—Refining fats or fatty oils by extraction
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/10—Refining fats or fatty oils by adsorption
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention relates to the technical field of animal oil refining, in particular to a refining method of horse oil. Crude horse is washed with water for three times to remove most impurity protein, and then passes by a filling column filled with an amino functional material, one aldehyde group in glutaraldehyde and the amino on the amino functional material are subjected to schiff's reaction, and another aldehyde group and the amino on the impurity protein are subjected to schiff's reaction to remove the impurity protein in the horse oil; finally, the horse oil is decolored and dehydrated to obtain the final refined horse oil. The refining method has simple steps, is easy to operate, avoids the complex refining operation of the traditional horse oil refining and loss of effective components in the horse oil, and is suitable for popularize and apply, and the cost is reduced.
Description
(1) technical field
The present invention relates to animal fat purification techniques field, particularly a kind of process for purification of house oil.
(2) background technology
The applicating history of house oil for a long time, modern more deep about the research of house oil, becomes for active component, the fat of house oilPoint, transdermal function, extracting method all carried out more detailed research. Research discovery, house oil has effect widely really: house oilThere is a stronger affine permeability to mucocutaneous, be easily absorbed by the skin, and there is the skin metabolism of acceleration, strengthen cell and liveThe effect such as power, anti aging effect, and color and smell very good, therefore multiplex house oil is made various U.S.s in recent yearsHold skin-protection product, as perfumed soap, toner, moisturiser, face cream, shampoo, hair conditioner, bath oil etc.; Medical aspect in addition, horseOil is also no lack of effective illness in inside and outside, Gynecology & Pediatrics Dept., such as flu, headache, constipation, rheumatic fever, neuralgia, gynaecology's sympotoms caused by cold factors, bitterlyWarp, allergic rhinitis etc., and mainly with coordinating other important prescriptions to use as well. Therefore, house oil has vast potential for future development.
The house oil of tradition decoction method, enzymatic isolation method or extract by solvents, have that acid number is too high, stink is heavier, albumen andThe impurity contents such as phosphatide are too high, pigment content is crossed the shortcomings such as high, therefore its refinery practice comprise come unstuck, depickling, decolouring, deodorization etc.Step. The patent that for example patent No. is CN102191127, complex operation step, operating cost is high, and active ingredient is lost serious.
Amino functional carrier can obtain conventionally by the following method: (1) utilizes natural products to obtain through simple processing, such as chitin, shitosan etc.; (2) direct polymerization amino functional base monomer; (3) first polymerization is with the monomer of inertia group,Carry out afterwards chemical modification; (4) by chemical reaction, be that other functional genes are converted into amino functional base etc. Double-functional group examinationAgent refers to the bifunctional group of two reactive sites, can be separated by again and hand between two nearer amino acid residuesConnection reaction. Glutaraldehyde cross-linking is that the amino generation nucleophilic addition in double-functional group aldehyde radical and amino functional material and protein molecular is anti-Should produce western Buddhist alkali (Schiffbase) and pass through covalent bonds.
(3) summary of the invention
The present invention, in order to make up the deficiencies in the prior art, provides the horse that a kind of step is simple, product quality is high, production cost is lowThe process for purification of oil.
The present invention is achieved through the following technical solutions:
A process for purification for house oil, taking thick house oil as handling object, comprises the steps:
(1) in thick house oil, add the distilled water of 80 DEG C to wash, centrifugal under 4000r/min after washing three times, get upper strataHouse oil;
(2) make house oil after centrifugal by being filled with the packed column of amino functional carrier and glutaraldehyde cross-linking agent, remove thick house oilIn the foreign protein that contains, obtain the house oil after chromatography;
(3) in house oil, add the atlapulgite that accounts for its weight 5%, 30min decolours at 60 DEG C;
(4) use drier to dewater to house oil, filter and obtain product.
It is thick that the present invention obtains through methods such as decoction method, cooking process, enzymatic isolation method or solvent extractions with fresh horse fat fat pieceHouse oil is handling object, after 3 washings, removes most of foreign protein, then passes through packed column; In packed column, fill amino meritCan material, there is schiff ' s with the amino on amino functional material and react in an aldehyde radical in glutaraldehyde, another aldehyde radical andThere is schiff ' s reaction in the amino on foreign protein, thereby the foreign protein in house oil is removed; Last house oil is through decolouringTo final refined horse oil.
More excellent scheme of the present invention is:
In step (2), amino functional carrier is chitin, shitosan or synthetic vectors.
In step (2), the cross-linking method of amino functional carrier and glutaraldehyde cross-linking agent is, by mass fraction 2% for shitosanAcetic acid solution dissolve, obtain chitosan gum liquid solution, solution is added drop-wise under condition of ice bath again to volume ratio after ultrasonic 10minIn mass fraction 7.5% sodium hydroxide solution/95.0% ethanolic solution for 4:1, form micelle, sealing is placed at 4 DEG C and preserves16h, takes out afterwards with physiological saline washing 3 times, and physiological saline soaks to be placed at 4 DEG C places 3h, and then suction filtration leaches micelle,Micelle is placed in to 30 DEG C, the glutaraldehyde solution of mass fraction 5% 2h that hardens, finally, with physiological saline washing, obtains crosslinked glueGrain.
In step (2), house oil with the flow velocity of 1ml/min to sample introduction in packed column.
In step (4), drier is anhydrous sodium sulfate, anhydrous magnesium sulfate, dead plaster or silica gel.
Step of the present invention is simple, and easy operating has reduced cost, has avoided numerous and diverse operation and the behaviour of traditional house oil refiningIn work, the loss of house oil active ingredient, is suitable for wide popularization and application.
(4) detailed description of the invention
Embodiment:
(1) preparation of thick house oil
Get fresh horse fat fat 30kg, be cut into the approximately fritter of 2 × 2cm, put into heatable container, after heating water bath to 50 DEG CPour meat grinder into and be twisted into meat gruel, then squeeze and filter obtains thick house oil 11.2kg;
(2) washing
In the thick house oil obtaining above, add 10L distilled water, water temperature is controlled at 80 DEG C, fully stirs, and leaves standstill 30min, 4000Rev/min centrifuging and taking upper strata grease, repeated washing 3 times, removes the water-solubility protein and other the water-soluble impurity that in thick house oil, contain;
(3) cross pillar
A. fill post, first by amino functional carrier and glutaraldehyde cross-linking: shitosan is dissolved with 2% acetic acid solution, obtain shitosanColloidal solution; Solution is added drop-wise to after ultrasonic 10min under condition of ice bath again the 7.5%NaOH solution that volume ratio is 4:1/In 95.0%vol ethanolic solution, form micelle, sealing is placed on 4 DEG C and preserves 16h, washs 3 times physiology after taking-up with physiological salineSaline sook is placed on 4 DEG C and places after 3h, and suction filtration leaches micelle, inserts in the glutaraldehyde of 30 DEG C 5%, and sclerosis 2h, uses physiology saltUnnecessary glutaraldehyde is removed in washing;
B. loading, with the flow velocity sample introduction of 1ml/min, and collects the house oil filtered solution flowing out.
(4) decolouring
In house oil, add the atlapulgite that accounts for its weight 5%, 30min decolours at 60 DEG C;
(5) dry
Use drier to dewater to house oil, filter and obtain product, wherein, drier be anhydrous sodium sulfate, anhydrous magnesium sulfate,Dead plaster or silica gel.
Claims (5)
1. a process for purification for house oil, taking thick house oil as handling object, is characterized by, and comprises the steps: that (1) is to thick house oilIn add the distilled water of 80 DEG C to wash, centrifugal under 4000r/min after washing three times, get upper strata house oil; (2) make centrifugal afterHouse oil by being filled with the packed column of amino functional carrier and glutaraldehyde cross-linking agent, obtain the house oil after chromatography; (3) toward house oilMiddle interpolation accounts for the atlapulgite of its weight 5%, and 30min decolours at 60 DEG C; (4) use drier to dewater to house oil, filterObtain product.
2. the process for purification of house oil according to claim 1, is characterized in that: in step (2), amino functional carrier is firstShell element, shitosan or synthetic vectors.
3. the process for purification of house oil according to claim 1, is characterized in that: in step (2), and amino functional carrier and pentaThe cross-linking method of dialdehyde crosslinking agent is, shitosan is dissolved with the acetic acid solution of mass fraction 2%, obtains chitosan gum liquid solution,Solution is added drop-wise to after ultrasonic 10min under condition of ice bath again mass fraction 7.5% sodium hydroxide solution that volume ratio is 4:1/In 95.0% ethanolic solution, form micelle, sealing is placed at 4 DEG C preserves 16h, takes out afterwards with physiological saline washing 3 times, physiologySaline sook is placed at 4 DEG C and places 3h, and then suction filtration leaches micelle, and micelle is placed in to 30 DEG C, the glutaraldehyde of mass fraction 5%The 2h that hardens in solution, finally, with physiological saline washing, obtains crosslinked micelle.
4. the process for purification of house oil according to claim 1, is characterized in that: in step (2), house oil is with 1ml/min'sFlow velocity is to sample introduction in packed column.
5. the process for purification of house oil according to claim 1, is characterized in that: in step (4), drier is anhydrous slufuric acidSodium, anhydrous magnesium sulfate, dead plaster or silica gel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510995070.XA CN105602717A (en) | 2015-12-28 | 2015-12-28 | Refining method of horse oil |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510995070.XA CN105602717A (en) | 2015-12-28 | 2015-12-28 | Refining method of horse oil |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105602717A true CN105602717A (en) | 2016-05-25 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510995070.XA Pending CN105602717A (en) | 2015-12-28 | 2015-12-28 | Refining method of horse oil |
Country Status (1)
| Country | Link |
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| CN (1) | CN105602717A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109666533A (en) * | 2019-01-25 | 2019-04-23 | 新疆天药生物科技有限公司 | Refined horse oil and its refining extracting method and application |
| CN111235190A (en) * | 2020-01-22 | 2020-06-05 | 新疆农业大学 | Horse oil rich in diglyceride and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1078490A (en) * | 1992-05-14 | 1993-11-17 | 吴风岑 | The production method of refined horse oil |
| CN1456648A (en) * | 2002-05-10 | 2003-11-19 | 曹泉海 | Horse fat extraction and use in industry |
| CN1540340A (en) * | 2003-10-25 | 2004-10-27 | 玉环县海洋生物化学有限公司 | Biologic chromatography gelatin and producing texhnique |
| CN102068965A (en) * | 2010-12-09 | 2011-05-25 | 浙江工业大学 | Method for preparing chitosan separation medium suitable for protein purification |
| CN104874362A (en) * | 2015-04-02 | 2015-09-02 | 中华人民共和国广州机场出入境检验检疫局 | Method for activating chitosan microspheres |
-
2015
- 2015-12-28 CN CN201510995070.XA patent/CN105602717A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1078490A (en) * | 1992-05-14 | 1993-11-17 | 吴风岑 | The production method of refined horse oil |
| CN1456648A (en) * | 2002-05-10 | 2003-11-19 | 曹泉海 | Horse fat extraction and use in industry |
| CN1540340A (en) * | 2003-10-25 | 2004-10-27 | 玉环县海洋生物化学有限公司 | Biologic chromatography gelatin and producing texhnique |
| CN102068965A (en) * | 2010-12-09 | 2011-05-25 | 浙江工业大学 | Method for preparing chitosan separation medium suitable for protein purification |
| CN104874362A (en) * | 2015-04-02 | 2015-09-02 | 中华人民共和国广州机场出入境检验检疫局 | Method for activating chitosan microspheres |
Non-Patent Citations (1)
| Title |
|---|
| 敬思群 等: "马油精炼工艺优选及抑菌性研究", 《食品工业科技》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109666533A (en) * | 2019-01-25 | 2019-04-23 | 新疆天药生物科技有限公司 | Refined horse oil and its refining extracting method and application |
| CN111235190A (en) * | 2020-01-22 | 2020-06-05 | 新疆农业大学 | Horse oil rich in diglyceride and preparation method thereof |
| CN111235190B (en) * | 2020-01-22 | 2023-11-07 | 新疆农业大学 | Horse oil rich in diglyceride and preparation method thereof |
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Application publication date: 20160525 |