CN105601357A - Application of chlorella extract in germination of cauliflower seeds, and preparation method of chlorella extract - Google Patents
Application of chlorella extract in germination of cauliflower seeds, and preparation method of chlorella extract Download PDFInfo
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- CN105601357A CN105601357A CN201510657894.6A CN201510657894A CN105601357A CN 105601357 A CN105601357 A CN 105601357A CN 201510657894 A CN201510657894 A CN 201510657894A CN 105601357 A CN105601357 A CN 105601357A
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Abstract
The invention discloses an application of chlorella extract in germination of cauliflower seeds, and a preparation method of the chlorella extract. The method concretely comprises the steps of preparation of the chlorella extract, and processing and culture of the cauliflower seeds. Industrial wastewater adopted as a chlorella medium provides various abundant nitrogen and phosphorus elements for chlorella and realizes sewage purification, so basis is provided for realizing industrial green emission reduction production. The chlorella extract is rich in various functional nutritional components, and provides green, pure-natural and various nutritional substances needed by germination of the cauliflower seeds, so the germination period of the seeds is shortened, and the germination rate of the seeds is improved.
Description
Technical field
The present invention relates to the application of chlorella extract at agricultural technology field, be specifically related to chlorella extractApplication and preparation method thereof in cauliflower seed is sprouted.
Background technology
Chlorella (ChlorellaIryrenoidosa) is under the jurisdiction of Chlorophyta Chlorphyta, ChlorococcaleChloroeoecales, the egg capsule algae Oocystaceae of section, Chlorella Chlorella are the common list in oceanCell algae, has Important Economic and is worth and huge application and potentiality to be exploited. Chlorella is natural in abundantProtein, lipid, dietary fibre, biotin, cellulose, unrighted acid, bioactive polysaccharide,Nucleic acid, vitamin, trace element, mineral matter, folic acid and chlorophyll. These special active ingredients havePrevent and treat peptic ulcer, antitumor, strengthen immunity, radioresistance, resisting pathogenic microbes, prevent and treat anaemia, fallThe health care such as blood fat and antiatherosclerosis and pharmacological action. The protein content of its dry powder more than 50%,Be good unicellular forage protein source, in feed, add the productivity that can improve pork pig, broiler and layerCan, be applied to aquatic feeds and can improve fish growth speed. Chlorella extract is with 500-1000 times of dilutions,Spray in the blade face such as vegetables, fruit tree, have strengthen plant photosynthesis, impel root growth vigorous, carryThe absorbability of height to nutrient and the effect of the resistance of raising to bacterium. Therefore, in recent years to chlorellaAttention rate also more and more higher. All further investigate from the various aspects such as cultivation, application of chlorella. Patent CN102586116B discloses " a kind of chlorella vulgaris and cultural method thereof and application ", this invention cultural methodSewage disposal and produce bioenergy and other byproducts (as, protein, cosmetic material and animal are raisedMaterial etc.) combine, reduce production costs, reach the comprehensive utilization of waste resource, realize the green of energy-saving and emission-reductionLook is produced. Patent CN103519035B discloses " a kind of preparation method of chlorella fermented type jelly " shouldInvention the present invention, taking chlorella as raw material, utilizes the high protein and the natural colouring matter that itself have, without interpolationEssence and pigment, the jelly making has natural green, the delicate fragrance of acidified milk, and sour-sweet suitable mouthfeel,There is again very high nutritive value simultaneously. Learn according to related data, in recent years, the grinding of China chlorellaStudy carefully focus in the biological culture of chlorella, biodiesel and in the research aspect health products, food, in agricultureThe research of industry technical field is very few.
In order to solve above-mentioned the deficiencies in the prior art part, primary and foremost purpose of the present invention is to provide and a kind of easily cultivates,The chlorella extract easily extracting. This invention improved cauliflower seed germination percentage, shortened seed germinationCycle.
Another object of the present invention is to provide the preparation method of above-mentioned chlorella extract.
A further object of the present invention is to provide above-mentioned chlorella extract to answer in cauliflower seed is sproutedWith.
Above-mentioned purpose of the present invention is to be achieved through the following technical solutions:
1, preparation liquid of glucose cultivate based on 120 DEG C, 1kpa, autoclaving 1.5h under condition, cooling afterAccess chlorella algae kind, is placed in incubator illumination cultivation, and cultivation temperature 25-30 DEG C, after incubation time 3dSpread cultivation to test tube in 1:1 ratio, grow to when dark green and further spread cultivation to 100ml conical flask;
2, the preparation of chlorella extract: get the chlorella liquid 30mL that growth conditions is good, density is 2.0 ×107CFU/ml, shakes up, and centrifugal 5min (2500r/min) is precipitated as algae mud after centrifugal. To chlorellaIn mud, add 95% ethanol to make suspension, add cellulase and pectase (ratio is 1:1) 2-6ml, enzymeSeparate 24h, 25 DEG C of hydrolysis temperatures. Enzymolysis carries out again centrifugal afterwards, time 10min, and rotating speed is 4000r/Min. Get supernatant, decompression distillation is removed alcohol solvent and is obtained chlorella extract;
3, the processing of cauliflower seed and cultivation: get in the same sizely, the cauliflower seed of full grains, with 2%Hydrogen peroxide sterilization 2 times, distilled water flushing 3 times, is then evenly placed in the glass culture dish that is covered with filter paper,Add chlorella extract to the seed that is mixed with 4% concentration to soak completely, put into incubator quiet in 25 DEG C of constant temperaturePut 24h, be placed on room temperature natral light cahure and germinate.
Described liquid of glucose culture medium is: food industrial wastewater 100ml, glucose 10.0g, trace unitElement.
Described trace element is FeC6H50·5H203.9g、NaEDTA4.35g、MnCI·4H202.188g、ZnS04·7H200.0278g、CoCl·6H200.0687g、CuS04·5H200.0102g、H3BO30.08gNa2MoO42.0g。
Described cellulase is 1.0 ten thousand units with the ratio of pectinase activity: 2.0 ten thousand units.
Through the immersion treatment of chlorella extract, cauliflower seed germination percentage is higher than 95%, and the germination cycle shortens2 to 3 days.
Chlorella extract promote the seed germination mechanism of action be chlorella extract contain amino acid, polysaccharide,The functional components such as protein, vitamin, has supplemented the needed nutritional labeling of during Seed Germination, activationSeed cell, promotes seed cell differentiation and growth, thereby impels seed germination.
The present invention has following beneficial effect:
1, the present invention adopts industrial wastewater as chlorella culture medium, for chlorella provides various abundant nitrogen phosphorusWhen element, reach the effect of purifying waste water, provide foundation for realizing Industrial Green reduction of discharging production.
2,, owing to being rich in various functional nutrient compositions in chlorella extract, sprout and provide for cauliflower seedThe natural various nutriments of required green pure, shorten the germination cycle, have improved percentage of seedgermination.
Detailed description of the invention
Embodiment 1
1, preparation liquid of glucose cultivate based on 120 DEG C, 1kpa, autoclaving 1.5h under condition, cooling afterAccess chlorella algae kind, is placed in incubator illumination cultivation, and 25 DEG C of cultivation temperature, press after incubation time 3d1:1 ratio spreads cultivation to test tube, grows to when dark green and further spreads cultivation to 100ml conical flask;
2, the preparation of chlorella extract: get the chlorella liquid 30mL that growth conditions is good, density is 2.0 ×107CFU/ml, shakes up, and centrifugal 5min (2500r/min) is precipitated as algae mud after centrifugal. To chlorellaIn mud, add 95% ethanol to make suspension, add cellulase and pectase (ratio is 1:1) 4ml, enzymolysis24h, 25 DEG C of hydrolysis temperatures. Enzymolysis carries out again centrifugal afterwards, time 10min, and rotating speed is 4000r/min.Get supernatant, decompression distillation is removed alcohol solvent and is obtained chlorella extract;
3, the processing of cauliflower seed and cultivation: choose in the same size, 100 cauliflower kinds of full grainsSon, with 2% hydrogen peroxide sterilization 2 times, distilled water flushing 3 times, is then evenly placed in the glass training that is covered with filter paperSupport in ware, add chlorella extract to the seed that is mixed with 4% concentration to soak completely, put into incubator in 25 DEG CConstant temperature leaves standstill 24h, is placed on room temperature natral light cahure and germinates.
Cauliflower seed germination percentage is 96% after measured, and the germination cycle is 4 days.
Embodiment 2
1, preparation liquid of glucose cultivate based on 120 DEG C, 1kpa, autoclaving 1.5h under condition, cooling afterAccess chlorella algae kind, is placed in incubator illumination cultivation, and 28 DEG C of cultivation temperature, press after incubation time 3d1:1 ratio spreads cultivation to test tube, grows to when dark green and further spreads cultivation to 100ml conical flask;
2, the preparation of chlorella extract: get the chlorella liquid 30mL that growth conditions is good, density is 2.0 ×107CFU/ml, shakes up, and centrifugal 5min (2500r/min) is precipitated as algae mud after centrifugal. To chlorellaIn mud, add 95% ethanol to make suspension, add cellulase and pectase (ratio is 1:1) 6ml, enzymolysis24h, 25 DEG C of hydrolysis temperatures. Enzymolysis carries out again centrifugal afterwards, time 10min, and rotating speed is 4000r/min.Get supernatant, decompression distillation is removed alcohol solvent and is obtained chlorella extract;
3, the processing of cauliflower seed and cultivation: choose in the same size, 150 cauliflower kinds of full grainsSon, with 2% hydrogen peroxide sterilization 2 times, distilled water flushing 3 times, is then evenly placed in the glass training that is covered with filter paperSupport in ware, add chlorella extract to the seed that is mixed with 4% concentration to soak completely, put into incubator in 25 DEG CConstant temperature leaves standstill 24h, is placed on room temperature natral light cahure and germinates.
Cauliflower seed germination percentage is 98% after measured, and the germination cycle is 5 days.
Comparative example 1
Choose in the same sizely, 100 cauliflower seeds of full grains, with 2% hydrogen peroxide sterilization 2 times, steamHeat up in a steamer water and rinse 3 times, be then evenly placed in the glass culture dish that is covered with filter paper, add common seed culture fluidSoak completely to seed, put into incubator and leave standstill 24h in 25 DEG C of constant temperature, be placed on room temperature natral light cahureGerminate.
Comparative example 2
Choose in the same sizely, 150 cauliflower seeds of full grains, with 2% hydrogen peroxide sterilization 2 times, steamHeat up in a steamer water and rinse 3 times, be then evenly placed in the glass culture dish that is covered with filter paper, add common seed culture fluidSoak completely to seed, put into incubator and leave standstill 24h in 25 DEG C of constant temperature, be placed on room temperature natral light cahureGerminate.
Described common seed culture fluid is: potassium nitrate 3g, calcium nitrate 5g magnesium sulfate 3g, ammonium phosphate 2g;Potassium sulfate 1g, di(2-ethylhexyl)phosphate hydrogen clock 1g, b diammonium disodium edta 100mg, ferrous sulfate 75mg; BoronAcid 30mg, manganese sulfate 20mg, zinc sulfate 5mg, copper sulphate 1mg, ammonium molybdate 2mg, distilled water 100mL.
The assay method of cauliflower seed germination percentage, germinating energy is as follows:
Germination percentage=(germinate and finish chitting piece number/sowing seed number) × 100%
Germinating energy=[the 2nd day chitting piece number/sowing seed number] × 100%；
In embodiment 1, comparative example 1, cauliflower seed germination percentage is as following table:
| Processing mode | Germination quantity | Germination percentage % |
| Embodiment 1 | 98 | 98 |
| Comparative example 1 | 82 | 82 |
In embodiment 1, comparative example 1, cauliflower seed germinating energy is as following table:
| Processing mode | Second day germination quantity | Germinating energy % |
| Embodiment 1 | 85 | 85 |
| Comparative example 1 | 60 | 60 |
In embodiment 2, comparative example 2, cauliflower seed germination percentage is as following table:
| Processing mode | Germination quantity | Germination percentage % |
| Embodiment 2 | 148 | 98.7 |
| Comparative example 2 | 84 | 84 |
In embodiment 2, comparative example 2, cauliflower seed germinating energy is as following table:
| Processing mode | Second day germination quantity | Germinating energy % |
| Embodiment 2 | 135 | 90 |
| Comparative example 2 | 118 | 78.7 |
Claims (4)
1. chlorella extract application and preparation method thereof in cauliflower seed is sprouted, is characterized in that comprisingFollowing steps:
1), preparation liquid of glucose cultivate based on 120 DEG C, 1kpa, autoclaving 1.5h under condition, cooling afterAccess chlorella algae kind, is placed in incubator illumination cultivation, and cultivation temperature 25-30 DEG C, after incubation time 3dSpread cultivation to test tube in 1:1 ratio, grow to when dark green and further spread cultivation to 100ml conical flask;
2), the preparation of chlorella extract: get the chlorella liquid 30mL that growth conditions is good, density is 2.0×107CFU/ml, shakes up, and centrifugal 5min (2500r/min) is precipitated as algae mud after centrifugal. To beadIn algae mud, add 95% ethanol to make suspension, add cellulase and pectase (ratio is 1:1) 2-6ml,Enzymolysis 24h, 25 DEG C of hydrolysis temperatures. Enzymolysis carries out again centrifugal afterwards, time 10min, and rotating speed is 4000r/ min. Get supernatant, decompression distillation is removed alcohol solvent and is obtained chlorella extract;
3), processing and the cultivation of cauliflower seed: get in the same sizely, the cauliflower seed of full grains, with 2%Hydrogen peroxide sterilization 2 times, distilled water flushing 3 times, is then evenly placed in the glass culture dish that is covered with filter paper,Add chlorella extract to the seed that is mixed with 4% concentration to soak completely, put into incubator quiet in 25 DEG C of constant temperaturePut 24h, be placed on room temperature natral light cahure and germinate.
2. chlorella extract according to claim 1 application and preparation thereof in cauliflower seed is sproutedMethod is characterized in that: liquid of glucose culture medium is: food industrial wastewater 100ml, glucose 10.0g,Trace element.
3. chlorella extract according to claim 2 application and preparation thereof in cauliflower seed is sproutedMethod is characterized in that: trace element is FeC6H50·5H203.9g、NaEDTA4.35g、MnCI·4H202.188g、ZnS04·7H200.0278g、CoCl·6H200.0687g、CuS04·5H200.0102g、H3BO30.08gNa2MoO42.0g。
4. chlorella extract according to claim 1 application and preparation thereof in cauliflower seed is sproutedMethod is characterized in that: step (2) cellulase is 1.0 ten thousand units with the ratio of pectinase activity: 2.0Ten thousand units.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107080045A (en) * | 2017-05-25 | 2017-08-22 | 甘肃凯源生物技术开发中心 | A kind of healthy pig feed of the cultivation containing chlorella and preparation method |
| CN107788202A (en) * | 2016-08-31 | 2018-03-13 | 深圳市菲赛迪投资发展有限公司 | A kind of biological feedstuff critical functionality nutrients and its production method |
| CN109006432A (en) * | 2018-08-01 | 2018-12-18 | 李慧 | Application of the chlorella in cultivation technique without soil |
| CN110214794A (en) * | 2019-05-10 | 2019-09-10 | 海盐县凌特生物科技有限公司 | Accelerate the preparation method for the regulator that seed is sprouted |
| CN110859192A (en) * | 2019-11-08 | 2020-03-06 | 宕昌县福江源药业科技有限责任公司 | Preparation for preventing and treating root rot of codonopsis pilosula and preparation method thereof |
| CN111635273A (en) * | 2020-05-14 | 2020-09-08 | 西宁市农业技术推广服务中心 | Soil fertilizer for improving soil activity and production method thereof |
| CN114451235A (en) * | 2022-02-22 | 2022-05-10 | 山西农业大学高粱研究所 | Germination accelerating breeding method for sorghum |
| CN114656301A (en) * | 2022-04-19 | 2022-06-24 | 烟台泓源生物肥料有限公司 | Chlorella biofertilizer capable of promoting growth and improving disease resistance |
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2015
- 2015-10-12 CN CN201510657894.6A patent/CN105601357A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107788202A (en) * | 2016-08-31 | 2018-03-13 | 深圳市菲赛迪投资发展有限公司 | A kind of biological feedstuff critical functionality nutrients and its production method |
| CN107080045A (en) * | 2017-05-25 | 2017-08-22 | 甘肃凯源生物技术开发中心 | A kind of healthy pig feed of the cultivation containing chlorella and preparation method |
| CN109006432A (en) * | 2018-08-01 | 2018-12-18 | 李慧 | Application of the chlorella in cultivation technique without soil |
| CN110214794A (en) * | 2019-05-10 | 2019-09-10 | 海盐县凌特生物科技有限公司 | Accelerate the preparation method for the regulator that seed is sprouted |
| CN110859192A (en) * | 2019-11-08 | 2020-03-06 | 宕昌县福江源药业科技有限责任公司 | Preparation for preventing and treating root rot of codonopsis pilosula and preparation method thereof |
| CN111635273A (en) * | 2020-05-14 | 2020-09-08 | 西宁市农业技术推广服务中心 | Soil fertilizer for improving soil activity and production method thereof |
| CN114451235A (en) * | 2022-02-22 | 2022-05-10 | 山西农业大学高粱研究所 | Germination accelerating breeding method for sorghum |
| CN114656301A (en) * | 2022-04-19 | 2022-06-24 | 烟台泓源生物肥料有限公司 | Chlorella biofertilizer capable of promoting growth and improving disease resistance |
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