CN105547145A - Super-resolution structure detection confocal coherent imaging device and imaging method thereof - Google Patents
Super-resolution structure detection confocal coherent imaging device and imaging method thereof Download PDFInfo
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- 238000001514 detection method Methods 0.000 title claims abstract description 43
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- 230000001427 coherent effect Effects 0.000 title claims abstract description 29
- 230000010354 integration Effects 0.000 claims abstract description 21
- 230000035945 sensitivity Effects 0.000 claims abstract description 10
- 238000005286 illumination Methods 0.000 claims abstract description 5
- 239000000523 sample Substances 0.000 claims description 37
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- 238000005259 measurement Methods 0.000 abstract description 6
- 238000010226 confocal imaging Methods 0.000 abstract description 2
- 238000010586 diagram Methods 0.000 description 22
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01B—MEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
- G01B9/00—Measuring instruments characterised by the use of optical techniques
- G01B9/02—Interferometers
- G01B9/02041—Interferometers characterised by particular imaging or detection techniques
- G01B9/02042—Confocal imaging
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01B—MEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
- G01B9/00—Measuring instruments characterised by the use of optical techniques
- G01B9/02—Interferometers
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- G01B9/02048—Rough and fine measurement
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Abstract
A super-resolution structure detection confocal coherent imaging device and an imaging method thereof relate to an imaging device and an imaging method thereof. The objective of the invention is to solve the problems that the resolution of the confocal spacing technology is difficult to improve and the confocal imaging is not clear. The device provided by the invention comprises a laser source; a collimation device, a beam splitter prism, a 1/4 wave plate, a scanning system, an illumination object lens, an industrial sample, a collection lens and a CCD detector are arranged in order in the ray propagation direction of the laser source; and integration is performed on the detection surface to change the luminous sensitivity corresponding to the detection position so as to allow the system CTF bandwidth to be enlarged. The spatial-cut-off frequency of a confocal coherent imaging system is improved, and the space frequency domain bandwidth is broadened, so that the transverse resolution of the imaging system is substantially improved; and moreover, the super-resolution structure detection confocal coherent imaging device and the imaging method thereof are suitable for the measurement field of the industry morphology imaging.
Description
Technical field
The present invention relates to imaging device and formation method thereof, be specifically related to a kind of super-resolution structure and detect confocal coherent imaging device and formation method thereof, belong to technical field of optical precision measurement.
Background technology
Optical microscopy is a kind of with a long history and very important in destructive technology, is widely used in the fields such as biological and material science.Confocal micro-measurement technology is a kind of three-dimensional optical microtechnic being applicable to micron and submicron-scale measurement.The chromatography ability of reflection-type confocal microscopic system makes it to seem very important in three-dimensional imaging field.
In middle and later periods the 1950's, confocal microscope is invented by Minsky, 1977, C.J.R.Sheppard and A.Choudhury illustrates confocal microscope system first under the effect of a pinhole mask, to sacrifice visual field for cost, lateral resolution is made to bring up to 1.4 times of same apertures simple microscope.After this, confocal micro-measurement technology is subject to common concern, becomes the important branch in micrology field.
But conventional confocal technology is subject to the impact of detector size always, the resolving power of confocal microscopy is difficult to improve.
Summary of the invention
The resolving power that the object of the invention is to solve existing confocal microscopy is difficult to improve, the unsharp problem of confocal imaging.
Technical scheme of the present invention is: a kind of super-resolution structure detects confocal coherent imaging device, comprise LASER Light Source, be provided with collimator and extender device, Amici prism, quarter wave plate, scanning system, illumination objective lens, production piece, collecting lens and ccd detector successively along LASER Light Source light transmition direction, the imaging process of whole light path is coherent imaging.
Described scanning system comprises scanning galvanometer, and scanning galvanometer changes beam deflection angle and scans at the object plane of production piece.
Detect the formation method of confocal coherent imaging device based on described a kind of super-resolution structure, comprise the following steps:
Step one, the probe function of ccd detector is utilized to obtain the integration light intensity of confocal system;
Step 2, obtain the three-dimensional amplitude points spread function of confocal system according to the integration light intensity described in step one;
Step 3, two-dimensional Fourier transform is carried out to the three-dimensional amplitude points spread function described in step 2, obtain the two-dimentional coherence transfer function of confocal system;
Step 4, the result of detection that will obtain, be reconstructed with three phase linearity separation methods, obtain super resolution image.
Described step one specifically comprises: described test surface adopts non-homogeneous detection mode, detection sensitivity coefficient in test surface is made to become Sine distribution, detection hot spot light intensity is multiplied by the detection coefficient of Sine distribution in the circular function that radius is Airy disk radius, obtains the integration light intensity of confocal system.
Described test surface adopts non-homogeneous detection mode, integration is carried out in test surface region, change the luminous sensitivity coefficient of corresponding detecting location, and then make probe function become Sine distribution, within the system, because probe function is Sine distribution, probe function frequency spectrum effective width compared with common confocal system increases, thus system CTF bandwidth can be made to increase, system transverse resolution significantly improves the transverse direction of fully excavating confocal system while playing the chromatography ability of reflection-type confocal microscopic system and differentiates potentiality.
The method that described step 2 obtains the three-dimensional amplitude points spread function of confocal system comprises: root of making even after converting the integration light intensity described in step one to Three dimensional convolution form, in thin sample is measured, equiphase in focal beam spot can be thought, then the expression distribution of amplitudes that the square root of light distribution that ccd detector collects can be similar to.
Described scanning system detects the invariant position of hot spot at test surface in scanning process.
Described detection hot spot light intensity root is the circular function of Airy disk radius at radius and realizes pin hole detection to light intensity integration in the circular function after calculating, and the pin hole before the detector utilizing the method to instead of in existing confocal system, achieves pin hole detecting function.
The present invention compared with prior art has following effect: super-resolution structure of the present invention detect confocal coherent imaging device with in, do not need the pin hole of test surface in common confocal system; Carry out integration in test surface specific region, change the luminous sensitivity of corresponding detecting location, probe function becomes Sine distribution, makes search coverage identical with common confocal middle pin hole region; The invention structure detection imaging method is combined with confocal relevant microscopic system, improve the spatial-cut-off frequency of confocal coherence imaging system, widen spatial frequency domain bandwidth, thus significantly improve imaging system transverse resolution, be applicable to the fields of measurement of industrial pattern imaging.
Accompanying drawing explanation
Fig. 1 is that superstructure of the present invention detects confocal coherent imaging device structural representation;
Fig. 2 is NA=0.1, λ=660nm, test surface pin hole radius
time, the test surface frequency spectrum normalization analogous diagram of substantially confocal relevant microscopic system;
Fig. 3 is NA=0.1, λ=660nm, test surface pin hole radius
probe function
Time, structure detects confocal coherent system test surface frequency spectrum normalization analogous diagram;
Fig. 4 is NA=0.1, λ=660nm, test surface pin hole radius
time, the CTF normalization analogous diagram of substantially confocal relevant microscopic system;
Fig. 5 is NA=0.1, λ=660nm, test surface pin hole radius
probe function
Time, structure detects confocal coherent system CTF normalization analogous diagram;
Fig. 6 is NA=0.1, λ=660nm, test surface pin hole radius
probe function
Time, structure detects confocal coherent system CTF with substantially confocal coherent system CTF at f
xdirection contrast normalization analogous diagram;
Fig. 7 is striped sample analogous diagram x direction and y direction being spaced apart 3.5um;
Fig. 8 be striped sample at NA=0.1, λ=660nm, test surface pin hole radius
time substantially confocal relevant microscopic system in the frequency spectrum analogous diagram that detects;
Fig. 9 be striped sample at NA=0.1, λ=660nm, test surface pin hole radius
time substantially confocal relevant microscopic system in imaging amplitude normalization analogous diagram;
Figure 10 be striped sample at NA=0.1, λ=660nm, test surface pin hole radius
probe function
Time, the confocal relevant microscopic system of structure detection in the frequency spectrum analogous diagram that detects;
Figure 11 be striped sample at NA=0.1, λ=660nm, test surface pin hole radius
probe function
Time, imaging amplitude normalization analogous diagram in the confocal aobvious relevant micro-system of structure detection;
Figure 12 be striped sample and its detect imaging in confocal microscope system at basic confocal microscope system and structure and contrast normalization analogous diagram at x direction amplitude;
In figure: 1, LASER Light Source, 2, collimator and extender device, 3, Amici prism, 4, collecting lens, 5, quarter wave plate, 6, ccd detector, 7, scanning system, 8, illumination objective lens, 9, production piece.
Embodiment
Accompanying drawings the specific embodiment of the present invention, a kind of super-resolution structure of the present invention detects confocal coherent imaging device, comprise LASER Light Source 1, be provided with collimator and extender device 2, Amici prism 3, quarter wave plate 5, scanning system 7, illumination objective lens 8, production piece 9, collecting lens 4 and ccd detector 6 successively along LASER Light Source 1 light transmition direction.
Described scanning system comprises scanning galvanometer, and scanning galvanometer changes beam deflection angle and scans at the object plane of production piece.
Detect the formation method of confocal coherent imaging device based on described a kind of super-resolution structure, comprise the following steps:
Step one, described step one specifically comprise: described test surface adopts non-homogeneous detection mode, detection sensitivity coefficient in test surface is made to become Sine distribution, detection hot spot light intensity is multiplied by the detection coefficient of Sine distribution in the circular function that radius is Airy disk radius, obtains the integration light intensity of confocal system:
Due to reality substantially confocal coherent system detection light intensity be test surface amplitude to the integration of test surface in limited range square, so the potentiality of the lateral resolution of confocal relevant microscopic system will be affected, namely the detection of finite size will cause system lateral resolution to be deteriorated.
Wherein D (r) is probe function, r in formula
1, r
s, r
2represent object space coordinate respectively; M
1, M
2represent illuminator and detection system enlargement ratio respectively; Scanning position coordinate and image space coordinate, h
1(r), o (r) and h
2r () represents illuminator point spread function respectively, thing function and detection system point spread function.
Described test surface adopts non-homogeneous detection mode, in test surface region, carry out integration, changes the luminous sensitivity coefficient of corresponding detecting location, and then makes probe function become Sine distribution.
Step 2, convert the integration light intensity described in step one to Three dimensional convolution form:
In formula
for Three dimensional convolution symbol, (2) formula evolution being obtained three-dimensional amplitude points spread function (APSF) h (r) is:
Step 3, suppose that axial defocusing amount z=0 carries out two-dimensional Fourier transform to three-dimensional amplitude points spread function (APSF) h (r), the two-dimentional coherence transfer function (CTF) of system can be obtained:
From CTF angle analysis, CTF and the probe function frequency spectrum product of collecting object lens cause the equivalent CTF bandwidth of collecting object lens to diminish, thus whole system CTF bandwidth diminishes.Under some detection condition, system CTF bandwidth is maximum, is 2 times of simple microscope.Under the infinitely great condition of detection area, system CTF bandwidth is the most minimum.
In basic confocal system, probe function is D (r)=circ (r/r
d) δ (z), its Fourier transform normalization analogous diagram is as shown in Figure 2.
Step 4, the result of detection that will obtain, be reconstructed with three phase linearity separation methods, obtain super resolution image.
Described scanning system 7 detects the invariant position of hot spot at test surface in scanning process.
Described test surface adopts non-homogeneous detection mode, makes detection sensitivity coefficient in test surface become Sine distribution, in test surface region, carries out integration, change the luminous sensitivity coefficient of corresponding detecting location, and then make probe function become Sine distribution.
Getting probe function in the present embodiment is:
F in formula
0represent the spatial frequency of cosine component in probe function;
represent the initial phase of different directions cosine component respectively; r
drepresent the radius of probe function.
In formula,
nA=0.1, λ=660nm, obtains integration light intensity to above formula, carries out Fourier transform after changing into convolution form:
In formula
represent probe function frequency spectrum; M, n represent x respectively, y direction frequency content.
Fig. 3 is probe function
fourier transform normalization analogous diagram.
Now, the CTF of confocal system becomes:
In above formula there is phase place aliasing in the cross one another part of five component frequency information, needs repeatedly to change
with
value, obtained image is reconstructed, removes the frequency information of a part of phase place overlapping area, obtain last super resolution image.
Described detection hot spot light intensity root is the circular function of Airy disk radius at radius and realizes pin hole detection to light intensity integration in the circular function after calculating.
Fig. 4 is NA=0.1, λ=660nm, test surface pin hole radius
time, the CTF normalization analogous diagram of substantially confocal relevant microscopic system;
Fig. 5 is NA=0.1, λ=660nm, test surface pin hole radius
probe function
Time, structure detects confocal coherent system CTF normalization analogous diagram;
Fig. 6 is NA=0.1, λ=660nm, test surface pin hole radius
probe function
Time, structure detects confocal coherent system CTF with substantially confocal coherent system CTF at f
xdirection contrast normalization analogous diagram;
By two curves in comparison diagram 6, can obviously find out, the confocal relevant microscopic system CTF cutoff frequency of structure detection is improved relative to substantially confocal relevant microscopic system.
Fig. 7 is striped sample analogous diagram x direction and y direction being spaced apart 3.3um;
Fig. 8 and Fig. 9 is the sample spectrum information detected in substantially confocal relevant microscopic system respectively, and sample imaging analogous diagram in substantially confocal relevant microscopic system.
Figure 10 and Figure 11 is the sample spectrum information detected in the confocal relevant microscopic system of structure detection respectively, and sample imaging analogous diagram in the confocal relevant microscopic system of structure detection.
Can find out that the highest sample frequency that the present embodiment can detect is apparently higher than substantially confocal relevant microscopic system by comparison diagram 8 and Figure 10.
By comparison diagram 9 and Figure 11, can find out that structure detects integral image resolving power that confocal ultra-resolution method obtains apparently higher than basic confocal microscope system, in conjunction with the comparing result of Figure 12, the present embodiment achieves the two-dimensional super-resolution of confocal relevant microscopic system, and the equivalent CTF bandwidth of confocal relevant microscopic system is expanded.
Claims (8)
1. a super-resolution structure detects confocal coherent imaging device, comprise LASER Light Source (1), it is characterized in that: be provided with collimator and extender device (2), Amici prism (3), quarter wave plate (5), scanning system (7), illumination objective lens (8), production piece (9), collecting lens (4) and ccd detector (5) along LASER Light Source (1) light transmition direction successively.
2. a kind of super-resolution structure detects confocal coherent imaging device according to claim 1, it is characterized in that: described scanning system (7) comprises scanning galvanometer, and scanning galvanometer changes beam deflection angle and scans at the object plane of production piece.
3. detect the formation method of confocal coherent imaging device based on super-resolution structure a kind of described in claim 1, it is characterized in that: comprise the following steps:
Step one, the probe function of ccd detector is utilized to obtain the integration light intensity of confocal system;
Step 2, obtain the three-dimensional amplitude points spread function of confocal system according to the integration light intensity described in step one;
Step 3, two-dimensional Fourier transform is carried out to the three-dimensional amplitude points spread function described in step 2, obtain the two-dimentional coherence transfer function of confocal system;
Step 4, the result of detection that will obtain, be reconstructed with three phase linearity separation methods, obtain super resolution image.
4. a kind of super-resolution structure according to claim 3 detects the formation method of confocal coherent imaging device, it is characterized in that: described step one specifically comprises: described test surface adopts non-homogeneous detection mode, detection sensitivity coefficient in test surface is made to become Sine distribution, detection hot spot light intensity is multiplied by the detection coefficient of Sine distribution in the circular function that radius is Airy disk radius, obtains the integration light intensity of confocal system.
5. a kind of super-resolution structure according to claim 4 detects the formation method of confocal coherent imaging device, it is characterized in that: described test surface adopts non-homogeneous detection mode, integration is carried out in test surface region, change the luminous sensitivity coefficient of corresponding detecting location, and then make probe function become Sine distribution.
6. a kind of super-resolution structure according to claim 3 detects the formation method of confocal coherent imaging device, it is characterized in that: the method that described step 2 obtains the three-dimensional amplitude points spread function of confocal system comprises: root of making even after converting the integration light intensity described in step one to Three dimensional convolution form.
7. a kind of super-resolution structure according to claim 3 detects the formation method of confocal coherent imaging device, it is characterized in that: described scanning system 7 detects the invariant position of hot spot at test surface in scanning process.
8. a kind of super-resolution structure according to claim 3 detects the formation method of confocal coherent imaging device, it is characterized in that: described detection hot spot light intensity root is multiplied by circular function that radius is Airy disk radius and realizes pin hole detection to light intensity integration in the circular function after calculating.
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Cited By (3)
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CN108535217A (en) * | 2018-04-08 | 2018-09-14 | 雄安华讯方舟科技有限公司 | optical coherence tomography system |
CN109883955A (en) * | 2019-03-04 | 2019-06-14 | 哈尔滨工业大学 | Obtain the device and method of the optimum structure probe function of structure detection micro imaging system |
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107085290A (en) * | 2017-06-12 | 2017-08-22 | 哈尔滨工业大学 | A kind of Laser Scanning Confocal Microscope parallel scan device and scan method based on scanning galvanometer and semiconductor laser |
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CN108535217A (en) * | 2018-04-08 | 2018-09-14 | 雄安华讯方舟科技有限公司 | optical coherence tomography system |
CN109883955A (en) * | 2019-03-04 | 2019-06-14 | 哈尔滨工业大学 | Obtain the device and method of the optimum structure probe function of structure detection micro imaging system |
CN109883955B (en) * | 2019-03-04 | 2021-10-08 | 哈尔滨工业大学 | Device and method for obtaining optimal structure detection function of structure detection microscopic imaging system |
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