CN105542759A - Method for preparing fluorescence carbon dots based on sweet potato extract - Google Patents
Method for preparing fluorescence carbon dots based on sweet potato extract Download PDFInfo
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- CN105542759A CN105542759A CN201511004643.4A CN201511004643A CN105542759A CN 105542759 A CN105542759 A CN 105542759A CN 201511004643 A CN201511004643 A CN 201511004643A CN 105542759 A CN105542759 A CN 105542759A
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- C09K11/08—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials
- C09K11/65—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials containing carbon
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Abstract
The invention discloses a method for preparing fluorescence carbon dots based on sweet potato extract. The fluorescence carbon dots are prepared by a hydrothermal method adopting sweet potato extract as a carbon source. The method comprises the following steps: adding the sweet potato extract into a reaction kettle and heating for several hours; and centrifuging the reaction liquid, filtering with a filtering membrane and dialyzing to obtain a solution of fluorescence carbon dots. According to the method disclosed by the invention, the adopted raw material comes from natural resources and is easily available; and the preparation method is simple and green and environmentally friendly. The obtained carbon dots have perfect water solubility and high fluorescence intensity and are applicable to the fields such as bio-labeling and cell imaging.
Description
Technical field
The invention belongs to technical field of nano material, relate to one using the sweet potato extraction solution as carbon source, adopt hydrothermal method to prepare the method for carbon point.
Background technology
Carbon point is the class Novel Carbon Nanomaterials that development in recent years is got up.After Xu in 2004 etc. adopt electrophoretic method to obtain carbon point from Single Walled Carbon Nanotube separation and purification first, the research about carbon point receives increasing concern.The grain diameter of carbon point is less than 10nm usually, has the optical property that fluorescence intensity is high, good light stability, resistance to photobleaching etc. are excellent.Because carbon point forms primarily of carbon, not containing metallic element, and its surface is containing the water soluble functional group such as hydroxyl and carboxyl, therefore carbon point has hypotoxicity and good water-soluble, thus make carbon point have desirable biocompatibility, have a wide range of applications in cell marking, cell imaging etc.
The preparation method of carbon point can be divided into " from top to bottom " and " from bottom to top " two kinds of methods substantially." from top to bottom " method is that macroscopical carbon structure material is prepared carbon point, as arc process, laser irradiation, electrochemical oxidation process, chemical oxidization method etc. by the method for fracture, pulverizing." from bottom to top " method is as precursor synthesis carbon point using organic molecule.The method comprises high temperature pyrolytic cracking (HTP), hydrothermal method, microwave method and supersonic method etc.Although develop the preparation method of numerous carbon point at present, often there is the shortcomings such as raw materials cost is high, conversion unit is expensive, severe reaction conditions, reaction process are loaded down with trivial details in these methods.Therefore, need to find a kind of cheaper starting materials and be easy to get, preparation process is simple, the carbon point preparation method of environmental protection.
Summary of the invention
Content of the present invention there are provided that a kind of raw material is easy to get, preparation method's environmental protection, carbon point preparation method simple to operate.Ipomoea batatas is the large economical crops in the world one, and its output is high, cheap and easy to get, and is rich in carbon and oxygen element, for the carbon point preparing high optical property provides possibility.
For achieving the above object, a kind of method preparing fluorescent carbon point based on the sweet potato extraction solution provided by the invention, take the sweet potato extraction solution as carbon source, prepares carbon point by hydrothermal method.The invention provides following technical scheme:
(1) with organizing puddler to be pounded pulpous state at a high speed after Ipomoea batatas chopping being added a certain amount of deionized water, heating, centrifugal, get supernatant liquor, be extracting solution;
(2) the sweet potato extraction solution is added in reactor react certain hour at a certain temperature;
(3) reaction solution is centrifugal, through membrane filtration, then with namely obtaining the carbon point solution of blue-fluorescence after dialysis tubing dialysis.
Wherein, in described step (1), the mass ratio of Ipomoea batatas fragment and deionized water is 1: 1-1: 3.5.
Wherein, in described step (1), Heating temperature is 60-90 DEG C, and heat-up time is 2-3.5h.
Wherein, in described step (2), the temperature of reaction of the sweet potato extraction solution in reactor is 160-220 DEG C, and the reaction times is 4-24h.
Wherein, in described step (3), centrifugal rotating speed is 12000r/min, and the time is 20min.
Wherein, in described step (3), the molecular weight cut-off of dialysis tubing is 3500Da, and dialysis time is 48h.
Compared with prior art, the beneficial effect that the present invention realizes: the present invention is that carbon source prepares fluorescent carbon point with the sweet potato extraction solution, and cheaper starting materials is easy to get, preparation method is simple, environmental protection; Carbon point good water solubility prepared by the present invention, good in optical property, is applicable to the fluorescent mark of cell.
Accompanying drawing explanation
Fig. 1 is the transmission electron microscope picture of carbon point prepared by embodiment 4
Fig. 2 is the infrared spectra of carbon point prepared by embodiment 4
Fig. 3 is the ultraviolet-visible absorption spectroscopy of carbon point prepared by embodiment 4
Fig. 4 is carbon point fluorescence maximum excitation and the emmission spectrum of embodiment 4 preparation
Fig. 5 is the fluorescence spectra emission spectrum of carbon point under different excitation wavelength prepared by embodiment 4
Embodiment
Embodiment 1
Take the new fresh sweet potatoes 100g cleaning peeling, add 100mL deionized water, with organizing puddler to smash to pieces at a high speed, the lower 60 DEG C of heating 2h of magnetic agitation.Centrifugal (10000r/min, 20min), gets supernatant liquor and is the sweet potato extraction solution.The sweet potato extraction solution is added in reactor, at 160 DEG C, react 4h.By reaction solution first centrifugal (10000r/min, 20min) with the membrane filtration using 0.22 μm after removing solid macrobead again.Gained solution molecular weight cut-off is the dialysis tubing dialysis 48h of 3500Da, finally obtains carbon point solution.
Embodiment 2
Take the new fresh sweet potatoes 100g cleaning peeling, add 170mL deionized water, with organizing puddler to smash to pieces at a high speed, the lower 80 DEG C of heating 3h of magnetic agitation.Centrifugal (10000r/min, 20min), gets supernatant liquor and is the sweet potato extraction solution.The sweet potato extraction solution is added in reactor, at 160 DEG C, react 14h.By reaction solution first centrifugal (10000r/min, 20min) with the membrane filtration using 0.22 μm after removing solid macrobead again.Gained solution molecular weight cut-off is the dialysis tubing dialysis 48h of 3500Da, finally obtains carbon point solution.
Embodiment 3
Take the new fresh sweet potatoes 100g cleaning peeling, add 350mL deionized water, with organizing puddler to smash to pieces at a high speed, the lower 90 DEG C of heating 3.5h of magnetic agitation.Centrifugal (10000r/min, 20min), gets supernatant liquor and is the sweet potato extraction solution.The sweet potato extraction solution is added in reactor, at 180 DEG C, react 12h.By reaction solution first centrifugal (10000r/min, 20min) with the membrane filtration using 0.22 μm after removing solid macrobead again.Gained solution molecular weight cut-off is the dialysis tubing dialysis 48h of 3500Da, finally obtains carbon point solution.
Embodiment 4
Take the new fresh sweet potatoes 100g cleaning peeling, add 350mL deionized water, with organizing puddler to smash to pieces at a high speed, the lower 80 DEG C of heating 3.5h of magnetic agitation.Centrifugal (10000r/min, 20min), gets supernatant liquor and is the sweet potato extraction solution.The sweet potato extraction solution is added in reactor, at 180 DEG C, react 18h.By reaction solution first centrifugal (10000r/min, 20min) with the membrane filtration using 0.22 μm after removing solid macrobead again.Gained solution molecular weight cut-off is the dialysis tubing dialysis 48h of 3500Da, finally obtains carbon point solution.
Embodiment 5
Take the new fresh sweet potatoes 100g cleaning peeling, add 350mL deionized water, with organizing puddler to smash to pieces at a high speed, the lower 80 DEG C of heating 3.5h of magnetic agitation.Centrifugal (10000r/min, 20min), gets supernatant liquor and is the sweet potato extraction solution.The sweet potato extraction solution is added in reactor, at 180 DEG C, react 24h.By reaction solution first centrifugal (10000r/mm, 20min) with the membrane filtration using 0.22 μm after removing solid macrobead again.Gained solution molecular weight cut-off is the dialysis tubing dialysis 48h of 3500Da, finally obtains carbon point solution.
Embodiment 6
Take the new fresh sweet potatoes 100g cleaning peeling, add 350mL deionized water, with organizing puddler to smash to pieces at a high speed, the lower 80 DEG C of heating 3.5h of magnetic agitation.Centrifugal (10000r/min, 20min), gets supernatant liquor and is the sweet potato extraction solution.The sweet potato extraction solution is added in reactor, at 200 DEG C, react 18h.By reaction solution first centrifugal (10000r/min, 20min) with the membrane filtration using 0.22 μm after removing solid macrobead again.Gained solution molecular weight cut-off is the dialysis tubing dialysis 48h of 3500Da, finally obtains carbon point solution.
Embodiment 7
Take the new fresh sweet potatoes 100g cleaning peeling, add 350mL deionized water, with organizing puddler to smash to pieces at a high speed, the lower 80 DEG C of heating 3.5h of magnetic agitation.Centrifugal (10000r/min, 20min), gets supernatant liquor and is the sweet potato extraction solution.The sweet potato extraction solution is added in reactor, at 220 DEG C, react 18h.By reaction solution first centrifugal (10000r/min, 20min) with the membrane filtration using 0.22 μm after removing solid macrobead again.Gained solution molecular weight cut-off is the dialysis tubing dialysis 48h of 3500Da, finally obtains carbon point solution.
Claims (6)
1. prepare a method for fluorescent carbon point based on the sweet potato extraction solution, it is characterized in that comprising the steps:
(1) with organizing puddler to be pounded pulpous state at a high speed after Ipomoea batatas chopping being added a certain amount of deionized water, heating, centrifugal, get supernatant liquor, be extracting solution;
(2) the sweet potato extraction solution is added in reactor react certain hour at a certain temperature;
(3) reaction solution is centrifugal, through membrane filtration, then with namely obtaining the carbon point solution of blue-fluorescence after dialysis tubing dialysis.
2. the method preparing fluorescent carbon point based on the sweet potato extraction solution according to claim 1, is characterized in that, the mass ratio of the Ipomoea batatas fragment described in step (1) and deionized water is 1: 1-1: 3.5.
3. the method preparing fluorescent carbon point based on the sweet potato extraction solution according to claim 1, is characterized in that, the Heating temperature described in step (1) is 60-90 DEG C, and heat-up time is 2-3.5h.
4. the method preparing fluorescent carbon point based on the sweet potato extraction solution according to claim 1, is characterized in that, the temperature of reaction of the sweet potato extraction solution described in step (2) in reactor is 160-220 DEG C, and the reaction times is 4-24h.
5. the method preparing fluorescent carbon point based on the sweet potato extraction solution according to claim 1, is characterized in that, the centrifugal rotating speed described in step (3) is 12000r/min, and the time is 20min.
6. the method preparing fluorescent carbon point based on the sweet potato extraction solution according to claim 1, is characterized in that, the molecular weight cut-off of the dialysis tubing described in step (3) is 3500Da, and dialysis time is 48h.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106395789A (en) * | 2016-09-27 | 2017-02-15 | 安徽师范大学 | Method for synthesizing carbon dots |
| CN106967425A (en) * | 2016-11-23 | 2017-07-21 | 云南省烟草公司文山州公司 | Using cigarette stalk as the preparation method and application of the fluorescent carbon quantum dot of raw material |
| CN114854402A (en) * | 2022-03-22 | 2022-08-05 | 桂林师范高等专科学校 | Preparation method of fluorescent nano material capable of promoting growth of cherokee rose group culture seedling |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106395789A (en) * | 2016-09-27 | 2017-02-15 | 安徽师范大学 | Method for synthesizing carbon dots |
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| CN106967425A (en) * | 2016-11-23 | 2017-07-21 | 云南省烟草公司文山州公司 | Using cigarette stalk as the preparation method and application of the fluorescent carbon quantum dot of raw material |
| CN114854402A (en) * | 2022-03-22 | 2022-08-05 | 桂林师范高等专科学校 | Preparation method of fluorescent nano material capable of promoting growth of cherokee rose group culture seedling |
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Application publication date: 20160504 |