CN105541603B - A method of separating natural falls off acid crude from abscisic acid fermentation liquid - Google Patents
A method of separating natural falls off acid crude from abscisic acid fermentation liquid Download PDFInfo
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- CN105541603B CN105541603B CN201510972958.1A CN201510972958A CN105541603B CN 105541603 B CN105541603 B CN 105541603B CN 201510972958 A CN201510972958 A CN 201510972958A CN 105541603 B CN105541603 B CN 105541603B
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
- C07C51/42—Separation; Purification; Stabilisation; Use of additives
- C07C51/43—Separation; Purification; Stabilisation; Use of additives by change of the physical state, e.g. crystallisation
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- Crystallography & Structural Chemistry (AREA)
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Abstract
A method of separating natural falls off acid crude from abscisic acid fermentation liquid, belong to biofermentation --- biochemical pesticides production field, abscisic acid fermentation liquid is obtained sheet frame filtrate through plate-frame filtering by this method, sheet frame filtrate ultrafiltration membrane, concentration film process obtain concentrate, by concentrate tune acid crystal, the acid crude that falls off that content is 80% ~ 90% is obtained after filtering, drying.This method extracted from fermentation liquid the acid crude that falls off have many advantages, such as it is high-efficient, at low cost.
Description
Technical field
The invention belongs to biofermentations --- and biochemical pesticides production field is related to one kind and divides from abscisic acid fermentation liquid
Method from natural abscisic acid.
Background technique
Abscisic acid is a kind of Endogenous hormones with comprehensive physiological function, is one of five big plant hormones, is plant
Important growth regulator is to balance plant endogenous hormones and the key factor in relation to the metabolism of growth activity substance, has and promotes to plant
Object Balance Absorption water, fertilizer and coordinate the ability that is metabolized in vivo, can Effective Regulation plant root/hat and nutrient growth and reproduction it is raw
It is long, play a significant role to the quality, the yield that improve crops.Abscisic acid is the extraordinary product of market prospects, in agriculture
Have broad application prospects in industry production, huge economic benefit and social benefit can be generated.Abscisic acid is in the market at present
Price is still very high, and either still international market, the acid product that falls off will all be chronically at the situation that supply falls short of demand at home.
In the method for acid crude that falls off obtained in traditional slave abscisic acid fermentation liquid, abscisic acid content in crude product is low, quality
Difference does not meet demand, while processing step is cumbersome, low efficiency.
Summary of the invention
The present invention for current natural abscisic acid low separation efficiency, at high cost, ropy deficiency, provide it is a kind of it is simple,
Efficiently separating natural falls off the method for acid crude from abscisic acid fermentation liquid.
The specific technical solution of the present invention is as follows.
A method of separating natural falls off acid crude from abscisic acid fermentation liquid, successively comprises the steps of:
(1) abscisic acid fermentation liquid obtains sheet frame filtrate through plate-frame filtering；
(2) sheet frame filtrate ultrafiltration membrane treatment obtains that liquid and ultrafiltration dialysis liquid is concentrated by ultrafiltration；
(3) ultrafiltration dialysis liquid is concentrated by the way that film is concentrated, and obtains concentrate；
(4) by concentrate tune acid crystal in step (3), the acid crude that falls off is obtained after filtering, drying.
Sheet frame filtrate pH is adjusted to 4.8 ~ 7.0 in step (1).
The molecular cut off of ultrafiltration membrane is 3000 ~ 30000 in step (2).
The molecular cut off of concentration film is 50 ~ 200 in step (3), and concentration film can be reverse osmosis membrane or nanofiltration membrane or both
Combination.
The pH of concentrate tune acid crystal is 1.5 ~ 3.0 in step (4).
Abscisic acid product content in step (4) is 80% ~ 90%.
The invention has the benefit that method of the invention is simply, conveniently, it is thick by the abscisic acid for adjusting acid crystal to obtain
Product, content are 80% ~ 90%, and product is loose, fine and smooth, and color is canescence, color, uniform granularity, can satisfy client for
Content is the demand of 80% or more crude product.The technological operation is simple, production cost is low, high-efficient.
Specific embodiment
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..
Embodiment 1.
Abscisic acid fermentation liquid obtains sheet frame filtrate through plate-frame filtering, and sheet frame filtrate pH is adjusted to 4.8, then uses molecular cut off
For 3000 ultrafiltration membrane treatments, obtain that liquid and dialyzate is concentrated by ultrafiltration, ultrafiltration dialysis liquid is 50 reverse osmosis membranes by molecular cut off
Be concentrated, obtain concentrate, concentrate is adjusted to pH 1.8 and is crystallized, filter, dry after obtain content be 83% abscisic acid it is thick
Product.
Embodiment 2.
Abscisic acid fermentation liquid obtains sheet frame filtrate through plate-frame filtering, and sheet frame filtrate pH is adjusted to 5.5, then uses molecular cut off
For 5000 ultrafiltration membrane treatments, obtain that liquid and dialyzate is concentrated by ultrafiltration, ultrafiltration dialysis liquid is 50 reverse osmosis membranes by molecular cut off
Be concentrated, obtain concentrate, concentrate is adjusted to pH 2.1 and is crystallized, filter, dry after obtain content be 84% abscisic acid it is thick
Product.
Embodiment 3.
Abscisic acid fermentation liquid obtains sheet frame filtrate through plate-frame filtering, and sheet frame filtrate pH is adjusted to 6.0, then uses molecular cut off
For 3000 ultrafiltration membrane treatments, obtain being concentrated by ultrafiltration liquid and dialyzate, ultrafiltration dialysis liquid by molecular cut off be 100 nanofiltration membranes into
Row concentration, obtain concentrate, concentrate be adjusted to pH 2.5 and is crystallized, filter, dry after obtain content be 89% abscisic acid it is thick
Product.
Embodiment 4.
Abscisic acid fermentation liquid obtains sheet frame filtrate through plate-frame filtering, and sheet frame filtrate pH is adjusted to 6.5, then uses molecular cut off
For 10000 ultrafiltration membrane treatments, obtain that liquid and dialyzate is concentrated by ultrafiltration, ultrafiltration dialysis liquid is 150 nanofiltration membranes by molecular cut off
Be concentrated, obtain concentrate, concentrate is adjusted to pH 3.0 and is crystallized, filter, dry after obtain content be 87% abscisic acid it is thick
Product.
Embodiment 5.
Abscisic acid fermentation liquid obtains sheet frame filtrate through plate-frame filtering, and sheet frame filtrate pH is adjusted to 7.0, then uses molecular cut off
For 30000 ultrafiltration membrane treatments, obtain that liquid and dialyzate is concentrated by ultrafiltration, ultrafiltration dialysis liquid is 100 nanofiltration membranes by molecular cut off
It is concentrated, obtains concentrate, concentrate is adjusted to pH2.7 crystallization, it is thick that the abscisic acid that content is 80% is obtained after filtering, drying
Product.
Embodiment 6.
Abscisic acid fermentation liquid obtains sheet frame filtrate through plate-frame filtering, and sheet frame filtrate pH is adjusted to 5.5, then uses molecular cut off
For 3000 ultrafiltration membrane treatments, obtain that liquid and dialyzate is concentrated by ultrafiltration, ultrafiltration dialysis liquid is 50 reverse osmosis membranes by molecular cut off
And molecular weight be 150 nanofiltration membranes be concentrated, obtain concentrate, concentrate be adjusted to pH 2.0 and is crystallized, filter, dry after obtain
The acid crude that falls off that content is 88%.
Embodiment 7.
Abscisic acid fermentation liquid obtains sheet frame filtrate through plate-frame filtering, and sheet frame filtrate pH is adjusted to 6.8, then uses molecular cut off
For 10000 ultrafiltration membrane treatments, obtain that liquid and dialyzate is concentrated by ultrafiltration, ultrafiltration dialysis liquid is 50 reverse osmosis membranes by molecular cut off
And molecular weight be 150 nanofiltration membranes be concentrated, obtain concentrate, concentrate be adjusted to pH 2.4 and is crystallized, filter, dry after obtain
The acid crude that falls off that content is 86%.
Claims (2)
- A kind of method of acid crude 1. separating natural from abscisic acid fermentation liquid falls off, it is characterised in that comprise the steps of:(1) abscisic acid fermentation liquid obtains sheet frame filtrate through plate-frame filtering, and sheet frame filtrate pH is adjusted to 4.8 ~ 7.0；(2) ultrafiltration membrane treatment that sheet frame filtrate molecular cut off is 3000 ~ 30000 obtains that liquid and ultrafiltration dialysis is concentrated by ultrafiltration Liquid；(3) ultrafiltration dialysis liquid is concentrated by the concentration film that molecular cut off is 50 ~ 200, obtains concentrate, the concentration Film is the combination of reverse osmosis membrane or nanofiltration membrane or both；(4) by concentrate tune acid crystal in step (3), the acid crude that falls off is obtained after filtering, drying, the concentrate tune acid PH is 1.5 ~ 3.0.
- The method of acid crude 2. a kind of separating natural from abscisic acid fermentation liquid according to claim 1 falls off, feature The content of acid crude of being to fall off is 80% ~ 90%.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510972958.1A CN105541603B (en) | 2015-12-23 | 2015-12-23 | A method of separating natural falls off acid crude from abscisic acid fermentation liquid |
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| CN201510972958.1A CN105541603B (en) | 2015-12-23 | 2015-12-23 | A method of separating natural falls off acid crude from abscisic acid fermentation liquid |
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| CN105541603A CN105541603A (en) | 2016-05-04 |
| CN105541603B true CN105541603B (en) | 2019-04-19 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106905142A (en) * | 2017-04-18 | 2017-06-30 | 四川龙蟒福生科技有限责任公司 | A kind of isolation and purification method of S abscisic acids |
| CN107047552A (en) * | 2017-04-18 | 2017-08-18 | 四川龙蟒福生科技有限责任公司 | The recoverying and utilizing method of accessory substance in a kind of S abscisic acids production |
| JP2020534815A (en) | 2017-09-18 | 2020-12-03 | エウロメッド・エセ・ア | Method for preparing plant extract of abscisic acid |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102241653A (en) * | 2011-07-08 | 2011-11-16 | 江西新瑞丰生化有限公司 | Membrane filtration and concentration technology of gibberellin |
| CN104817452A (en) * | 2014-12-19 | 2015-08-05 | 三达膜科技（厦门）有限公司 | Method for extracting abscisic acid from abscisic acid fermentation liquor |
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- 2015-12-23 CN CN201510972958.1A patent/CN105541603B/en active Active
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102241653A (en) * | 2011-07-08 | 2011-11-16 | 江西新瑞丰生化有限公司 | Membrane filtration and concentration technology of gibberellin |
| CN104817452A (en) * | 2014-12-19 | 2015-08-05 | 三达膜科技（厦门）有限公司 | Method for extracting abscisic acid from abscisic acid fermentation liquor |
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Address after: 331399 Salt Chemical Industry City, Xingan County, Ji'an City, Jiangxi Province Patentee after: Jiangxi new Ruifeng biochemical Limited by Share Ltd Address before: 331399 Salt Chemical Industry City, Xingan County, Ji'an City, Jiangxi Province Patentee before: Jiangxi Xinruifeng Biochemical Co., Ltd. |
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