CN105535365A - Component medicine for generating essence and tonifying yang and application thereof - Google Patents

Abstract

The invention provides a component medicine for generating essence and tonifying yang. The medicine is prepared from radix rehmanniae preparata, radix polygoni multiflori preparata, rhiizoma dioscoreae, fructus lycii, morinda officinalis how, cistanche salsa, herba epimedii, eucommia bark, fructus psoraleae, radix dipsaci, roots of bidentate achyranthes, poria cocos, lotus seeds, semen euryales, cornus officinalis, fructus schizandrae, polygala roots, calamendiol and fennel fruit extracts. The invention provides a medicine combination used for tonifying yang of men and treating male impotence. Compared with a traditional five-seed offspring-multiplying medicine combination, the medicine combination has a better curative effect.

Description

A kind of essence generating and YANG strengthening prescription medicine and application thereof

Technical field

The present invention relates to a kind of Chinese prescription medicine, relate to a kind of for essence generating and YANG strengthening prescription medicine and application thereof in particular.

Technical background

Cause the cause of disease more complicated of impotence due to deficiency of the kidney.Now, by a large amount of clinical practices and epidemiological investigation, find that sexual impotence is real how empty few, morbidity internal organs relate to the five internal organs centered by Liver and kidney.Kidney storing essence, main reproduction.Congenital defect, or take good care of inappropriate, sexual strain, prolonged illness is touching, all can cause deficiency of kidney-essence, deficiency of kidney-YIN.

The traditional Chinese medical science " Plain Questions six save Tibetan resemble fall " medium cloud: " kidney person, mainly stings, and the dominator of storing essence, smart part also." sperm source is in kidney.Cloud again in " Plain Questions ancient times innocence opinion ": " sixteen, kidney qi is contained, the menarche, spermatic ejeculation, negative and positive and, therefore can son be had." ZHU Dan-xi cloud: " there is the weak person that can not become tire of the empty essence of essence." the visible traditional Chinese medical science thinks that the virtual loss of the essence that kidney institute hides causes the basic reason of azoospermia, is also to adopt the kidney invigorating and essence nourishing to be the foundation of few smart, the azoospermia of main treatment, therefore treatment azoospermia is main mainly with the kidney invigorating.Radix Rehmanniae Preparata, Fructus Corni, Rhizoma Dioscoreae nourishing kidney mend essence; Fructus Lycii, Radix Achyranthis Bidentatae invigorating the liver and kidney, strong waist knee joint, the in love product of Colla cornus cervi, Colla Plastri Testudinis flesh and blood are with benefiting essence and marrow, grow Kidney-Yin, compatibility Semen Cuscutae warming Yang and benefiting Yin, closes Colla cornus cervi " treating YIN within YANG " simultaneously, makes the generation of cloudy essence in kidney have yang-energy as the lively power of change continuously.Full side is to the generation promoting sperm, and the quantity of raising sperm, the energy of enhancing sperm have good effect.Immune infertility is divided into kidney yin deficiency type, kidney yang deficiency syndrome, damp-heat type to carry out determination for the treatment of based on pathogenesis obtained through differentiation of symptoms and signs.The traditional Chinese medical science thinks kidney storing essence, main reproduction, and " the strange sick opinion of Plain Questions " is said: " collaterals of the uterus person, lies in kidney." kidney yang deficiency syndrome due to suffer from a deficiency of the kidney punching appoint lose support, menoxenia due to insufficiency of CHONG meridian; Kidney yin deficiency type, due to deficiency of YIN-blood, causes yang-energy partially prosperous, sea of blood intrinsic heat; Damp-heat type is due to damp invasion of lower energizer collaterals of the uterus, therefore loss of fecundity of a specified duration of wedding.So middle treatment is with the kidney invigorating, damp eliminating heat.Blood stasis hinders also to wed loss of fecundity of a specified duration in vessels of the uterus, therefore often adds blood circulation promoting and blood stasis dispelling Chinese medicine in treatment.Modern pharmacology proves: " kidney-nourishing tcm drug " has the effect of immunity moderation function.Poria, Radix Codonopsis, Fructus Lycii, Rhizomadioscoreae, the Rhizoma Atractylodis Macrocephalae, Herba Epimedii etc. have immunity moderation function equally.Radix Glycyrrhizae, Radix Angelicae Sinensis, Rhizoma Chuanxiong, capillary wormwood, Radix Scutellariae, Rhizoma Coptidis, Radix Salviae Miltiorrhizae, Radix Paeoniae Rubra, Cortex Moutan have the effect suppressing antibody to produce, and Rhizoma Coptidis, Cortex Phellodendri also have immunoregulation effect.

Summary of the invention

For solving the problem, the invention provides a kind of essence generating and YANG strengthening prescription medicine, described prescription is made up of the extract of Radix Rehmanniae Preparata, Radix Polygoni Multiflori Preparata, Rhizoma dioscoreae, Fructus Lycii, Radix Morindae Officinalis, Herba Cistanches, Herba Epimedii, the Cortex Eucommiae, Fructus Psoraleae, Radix Dipsaci, Radix Achyranthis Bidentatae, Poria, Semen Nelumbinis, Semen Euryales, Fructus Corni, Fructus Schisandrae Chinensis, Radix Polygalae, Rhizoma Acori Graminei, Fructus Foeniculi.

The weight ratio of described Radix Rehmanniae Preparata, Radix Polygoni Multiflori Preparata, Rhizoma dioscoreae, Fructus Lycii, Radix Morindae Officinalis, Herba Cistanches, Herba Epimedii, the Cortex Eucommiae, Fructus Psoraleae, Radix Dipsaci, Radix Achyranthis Bidentatae, Poria, Semen Nelumbinis, Semen Euryales, Fructus Corni, Fructus Schisandrae Chinensis, Radix Polygalae, Rhizoma Acori Graminei and Fructus Foeniculi is 1-10:1-10:1-10:1-10:1-10:1-10:1-10:1-10:1-10:1-10:1-10:1-10:1-10:1-10:1-10:1-10:1-10:1-10:1-10.

The weight ratio of described Radix Rehmanniae Preparata, Radix Polygoni Multiflori Preparata, Rhizoma dioscoreae, Fructus Lycii, Radix Morindae Officinalis, Herba Cistanches, Herba Epimedii, the Cortex Eucommiae, Fructus Psoraleae, Radix Dipsaci, Radix Achyranthis Bidentatae, Poria, Semen Nelumbinis, Semen Euryales, Fructus Corni, Fructus Schisandrae Chinensis, Radix Polygalae, Rhizoma Acori Graminei and Fructus Foeniculi is 4:4:4:4:3:3:3:3:3:3:3:3:3:3:3:3:2:2:2.

Described essence generating and YANG strengthening prescription medicine is preparing the application in Yang invigorating medicine.

The application of described essence generating and YANG strengthening prescription medicine in preparation treatment impotence medicine.

Advantageous Effects of the present invention is: the invention provides a kind of drug regimen for male Yang tonifying and treatment impotence, the more traditional five-seed Yanzong drug regimen curative effect of this drug regimen is better.

Detailed description of the invention

The activity of embodiment 1 prescription 1 is investigated

According to the formula recorded in claims, the weight ratio being 4:4:4:4:3:3:3:3:3:3:3:3:3:3:3:3:2:2:2 by the weight ratio of Radix Rehmanniae Preparata, Radix Polygoni Multiflori Preparata, Rhizoma dioscoreae, Fructus Lycii, Radix Morindae Officinalis, Herba Cistanches, Herba Epimedii, the Cortex Eucommiae, Fructus Psoraleae, Radix Dipsaci, Radix Achyranthis Bidentatae, Poria, Semen Nelumbinis, Semen Euryales, Fructus Corni, Fructus Schisandrae Chinensis, Radix Polygalae, Rhizoma Acori Graminei and Fructus Foeniculi takes medical material, with the alcohol reflux 1 hour of 3-5 60-70% doubly, reclaim extracting solution, concentrate drying extracting solution, prepares group I.

According to the formula recorded in claims, the weight ratio being 10:10:10:10:8:8:8:8:8:8:8:8:8:8:8:8:1:1:1 by the weight ratio of Radix Rehmanniae Preparata, Radix Polygoni Multiflori Preparata, Rhizoma dioscoreae, Fructus Lycii, Radix Morindae Officinalis, Herba Cistanches, Herba Epimedii, the Cortex Eucommiae, Fructus Psoraleae, Radix Dipsaci, Radix Achyranthis Bidentatae, Poria, Semen Nelumbinis, Semen Euryales, Fructus Corni, Fructus Schisandrae Chinensis, Radix Polygalae, Rhizoma Acori Graminei and Fructus Foeniculi takes medical material, with the alcohol reflux 1 hour of 3-5 60-70% doubly, reclaim extracting solution, concentrate drying extracting solution, prepares experimental group II.

According to the formula recorded in claims, the weight ratio being 2:2:2:2:1:1:1:1:1:1:1:1:1:1:1:1:9:9:9 by the weight ratio of Radix Rehmanniae Preparata, Radix Polygoni Multiflori Preparata, Rhizoma dioscoreae, Fructus Lycii, Radix Morindae Officinalis, Herba Cistanches, Herba Epimedii, the Cortex Eucommiae, Fructus Psoraleae, Radix Dipsaci, Radix Achyranthis Bidentatae, Poria, Semen Nelumbinis, Semen Euryales, Fructus Corni, Fructus Schisandrae Chinensis, Radix Polygalae, Rhizoma Acori Graminei and Fructus Foeniculi takes medical material, with the alcohol reflux 1 hour of 3-5 60-70% doubly, reclaim extracting solution, concentrate drying extracting solution, prepares experimental group III.

According to the formula recorded in claims, the weight ratio being 6:6:6:6:9:9:9:9:9:9:9:9:2:2:2:9:1:1:1 by the weight ratio of Radix Rehmanniae Preparata, Radix Polygoni Multiflori Preparata, Rhizoma dioscoreae, Fructus Lycii, Radix Morindae Officinalis, Herba Cistanches, Herba Epimedii, the Cortex Eucommiae, Fructus Psoraleae, Radix Dipsaci, Radix Achyranthis Bidentatae, Poria, Semen Nelumbinis, Semen Euryales, Fructus Corni, Fructus Schisandrae Chinensis, Radix Polygalae, Rhizoma Acori Graminei and Fructus Foeniculi takes medical material, with the alcohol reflux 1 hour of 3-5 60-70% doubly, reclaim extracting solution, concentrate drying extracting solution, prepares experimental group IV.

The activity of erection disturbance (sexual impotence) disease is investigated

The male 2 monthly age SD rats of healthy SPF level, about body weight 250g, confirms all have normal sexual function through mating test.Normal nursing, puts after laboratory conforms 1 week and tests.Adopt essence generating and YANG strengthening prescription medicine group I described above, II, III, IV group (hereinafter referred to as: I group, II group, III group and IV group), five subgroups adopt commercially available Wuziyanzong tablets (2010 editions " Chinese Pharmacopoeia " first); Become concentration be respectively 1.6g (crude drug)/ml with distilled water dissolving, dilution in proportion during use, prostatitis element E1 injected dose every day is 2 μ g/kg.

Low-oxygen environment model prepare: rat feed flint glass make hypoxia cabin in, free diet, day alternates with night raise.Cabin body bulk 600L, holds 14 mouse cages, feeds 5 rats in each mouse cage.Humiture: temperature 22-28 DEG C, humidity 50%-60% in the built-in Hygrothermograph in cabin, two window air conditioner control cabinets.Be filled with nitrogen oxygen mixed gas according to predetermined value in cabin, form the normal pressure low oxygen environment of 10% oxygen concentration.Place discolour silica gel absorbing moisture in hypoxia cabin, the carbon dioxide that rats breathing produces is absorbed by sodica calx, and the carbon dioxide volume number in maintenance cabin is lower than 3%.Matched group is raised in home, and other conditions are identical.Each group of rat was put to death after 6 weeks, got stage casing corpus cavernosum tissue and tested.

Get rat 80, after adaptability raises 1 week, be divided into 8 groups at random by weight, be respectively normal oxygen group group, model group, prostate E1 group, five subgroups, IV group, III group, II group, I group, often organize 10.

Corresponding experimental period is weighed, and inspection box conforms 10min.Every rat to relax skin place injection apomorphine at neck, and with normal saline and the preparation of 0.5% vitamin C injection, by l00 μ g/kg administration, observe 30min at once after administration, record penis is with or without erection and erection number of times.Judge index: Glans penis hyperemia and end body of penis appear as erection once.

Pcr amplification reaction process is under 75V voltage conditions, carry out electrophoretic analysis, after ethidium bromide staining, its product utilization Labworks image acquisition and analysis software carries out scanning analysis respectively, and result is expressed as each gene mRNA relative expression levels with the Reinhoit Zahl of rat α-actin, OPN gene and internal reference β-actin photograph.Utilize rat α-actin, the OPN and internal reference β-actin sequence that have reported in NCBI gene database, adopt Oligo6.71 Primer Analysis Software to design.

Adopt SPSS13.0 statistical package to add up, each group data acquisition mean adds standard deviation represent, adopt t inspection to carry out significance analysis according to data information character, inspection level is with P ﹤ 0.05 for there being statistical significance, and P ﹤ 0.01 has significance for difference.

Namely experimental group rat just for one day in hypoxia cabin has obvious hypoxic conditions to occur, shows as lassitude, rapid breathing, anorexia, body weight increases slowly, even lose weight.After 6 weeks, each group rat injection APO evaluates its erection function, normal oxygen group is that (5.31 ± 1.77) are secondary, hypoxia group is that (1.43 ± 0.89) are secondary, IV group is that (3.42 ± 1.35) are secondary, III group is that (3.62 ± 1.31) are secondary, II group is that (4.53 ± 1.23) are secondary, and I group is that (5.42 ± 0.85) are secondary.Model group compares with matched group, and erection number of times obviously reduces (P ﹤ 0.001), and the success of hypoxia experiments Animal Model is described.

The impact of α-actin and OPN relative expression quantity is as shown in table 1.Compare with normal oxygen group, the α-actin content of hypoxia model group is remarkable.Reduce (P ﹤ 0.01), OPN content obviously raises (P ﹤ 0.01).Compare with hypoxia model group, five subgroups, IV group, essence generating and YANG strengthening prescription medicine I, II, III group of α-actin content rising (P ﹤ 0.05), wherein I group of content rises obviously (P ﹤ 0.01); OPN content reduces (P ﹤ O.05), and wherein I, II group of content declines obviously (P ﹤ 0.O1).

Table 1 essence generating and YANG strengthening prescription medicine is on the impact of hypoxia CCSM α-actin, OPN relative expression quantity

Note: compare with normal oxygen model group, P ﹤ 0.05; Compare with hypoxia group, * * P ﹤ 0.01

Although smooth muscle only accounts for 45% in corpus cavernosum tissue, it plays an important role in adjustment erection and maintenance are erected, and is the main effects device position of penile nerve regulation and control.Therefore, the research about CCSMC seems particularly important.According to the difference of 26S Proteasome Structure and Function, smooth muscle is divided into shrinkage type and synthesis type two type by people.Smooth muscle cell is converted into synthesis type from shrinkage type and obtains multiplication capacity, and this process is called Phenotypic Change (phenotypicmodulation).Under pathological conditions, the Phenotypic Change of cell has d cell biological significance to body.At acute pathological changes or chronic disease initial stage, the cell of Phenotypic Change carries out necessary tissue repair by compensation responses such as propagation and extracellular matrix secretions (ECM), is favourable to body.But in the progression of chronic disease, due to paathogenic factor continuous action, the Phenotypic Change of cell often lose regulation and control and cause cell hyperproliferation or hypertrophy, secretion and degraded ECM ability unbalance, some cell even can be converted into another kind of cell phenotype completely, and these cells are by active participate, promotion or amplify pathological changes process.

Research finds: α-actin (α-actin) dyeing in CCSMC obviously, infer that the contractile element of corpus cavernosum smooth muscle is similar to the contractile element of vascular smooth muscle accordingly, CCSMC should have similar contraction diastolic function to vascular smooth muscle cell.Smooth muscle only α-actin globulin is the protein of ubiquitous rich content in SMC, in predominant expression in shrinkage type cell, and content is very micro-in synthesis type cell, it is shrink the important symbol of phenotype that smooth muscle α-actin expresses, and is also the shrinkage type marker protein applied.And osteopontin (OPN) is the significant albumen of synthesis type, research shows, the vascular smooth muscle high expressed OPNmRNA of synthesis type, and find with differential display technique, OPN is the marker gene of vascular smooth muscle contraction type to synthesis type Phenotypic Change.In view of the critical role of α-actin and OPN in vascular smooth muscle Phenotypic examination, this experiment observes essence generating and YANG strengthening prescription medicine on the impact of smooth muscle Phenotypic Change by detecting these two specific markers.

The investigation of effect of warming kidney and strengthening yang

Get mice 80, male, body weight 18-22g, is divided into 8 groups at random, only often organizes 1O.Except blank group intramuscular injection 0.9% sodium chloride injection, all the other respectively organize equal intramuscular injection oxidation cortisone 0.25mg/10g, and every day 1 time, continuous 10 days, the 11st day started gastric infusion, every day 1 time, blank group and model of yang asthenia group gavage deionized water 10ml/kg; 3rd group of positive controls, gavage methyltestosterone tablets 1mg/kg; Essence generating and YANG strengthening prescription medicine I, II, III, IV group of corresponding gavage (crude drug) 2.00g/kg; The commercially available Wuziyanzong tablets of five subgroup gavages (2010 editions " Chinese Pharmacopoeia " first) crude drug) 2.00g/kg; Continuous 10 days, at the 9th day of administration, O.5h mice is put in YL-1A Multifunctional mouse autonomic activities monitor after administration and adapts to 15min, by operation instruction counting mice 10min autonomic activities number of times.Fasting 12h before last administration, 0.5h after administration, claim Mouse Weight, Omron electric body-temperature measurement anus temperature, survey frozen water swimming time.

Measurement data adopts t inspection, with P<0.05 for there being significant difference.

The impact (n=10) that table 2 increases kidney-yang deficiency model Mouse Weight

Table 3 is putd question to kidney-yang deficiency model mice and the impact of the free movable number of times of mice

Table 4 is on the impact of mice with kidney-yang deficiency low temperature swimming exhaustion time

As described in table 2, table 3, table 4.Essence generating and YANG strengthening prescription medicine I, II, III and IV group all can make Mouse Weight increase, after medication treatment, methyltestosterone tablets and essence generating and YANG strengthening prescription medicine I, II, III and IV group of mice 10min autonomic activities number of times are all significantly higher than model control group (P<0.05); Methyltestosterone tablets and essence generating and YANG strengthening prescription medicine I group of mouse temperature are all significantly higher than model control group (P ﹤ 0.05).

This pharmacological evaluation shows, this product has obvious potentiation to spontaneous activity in mice, and kidney-yang deficiency model Mouse Weight is increased, and body temperature raises, and can extend the mice with kidney-yang deficiency low temperature swimming exhaustion time.Prompting essence generating and YANG strengthening prescription medicine can improve the effect of Mouse Kidney yang deficiency sign, has good therapeutical effect, have QI invigorating yang invigorating functions to sexual impotence.

Claims (5)

1. an essence generating and YANG strengthening prescription medicine, is characterized in that: described prescription is made up of the extract of Radix Rehmanniae Preparata, Radix Polygoni Multiflori Preparata, Rhizoma dioscoreae, Fructus Lycii, Radix Morindae Officinalis, Herba Cistanches, Herba Epimedii, the Cortex Eucommiae, Fructus Psoraleae, Radix Dipsaci, Radix Achyranthis Bidentatae, Poria, Semen Nelumbinis, Semen Euryales, Fructus Corni, Fructus Schisandrae Chinensis, Radix Polygalae, Rhizoma Acori Graminei and Fructus Foeniculi.

2. essence generating and YANG strengthening prescription medicine according to claim 1, is characterized in that: the weight ratio of described Radix Rehmanniae Preparata, Radix Polygoni Multiflori Preparata, Rhizoma dioscoreae, Fructus Lycii, Radix Morindae Officinalis, Herba Cistanches, Herba Epimedii, the Cortex Eucommiae, Fructus Psoraleae, Radix Dipsaci, Radix Achyranthis Bidentatae, Poria, Semen Nelumbinis, Semen Euryales, Fructus Corni, Fructus Schisandrae Chinensis, Radix Polygalae, Rhizoma Acori Graminei and Fructus Foeniculi is 1-10:1-10:1-10:1-10:1-10:1-10:1-10:1-10:1-10:1-10:1-10:1-10:1-10:1-10:1-10:1-10:1-10:1-10:1-10.

3. essence generating and YANG strengthening prescription medicine according to claim 2, is characterized in that: the weight ratio of described Radix Rehmanniae Preparata, Radix Polygoni Multiflori Preparata, Rhizoma dioscoreae, Fructus Lycii, Radix Morindae Officinalis, Herba Cistanches, Herba Epimedii, the Cortex Eucommiae, Fructus Psoraleae, Radix Dipsaci, Radix Achyranthis Bidentatae, Poria, Semen Nelumbinis, Semen Euryales, Fructus Corni, Fructus Schisandrae Chinensis, Radix Polygalae, Rhizoma Acori Graminei and Fructus Foeniculi is 4:4:4:4:3:3:3:3:3:3:3:3:3:3:3:3:2:2:2.

4. the arbitrary described essence generating and YANG strengthening prescription medicine of claim 1-3 is preparing the application in Yang invigorating medicine.

5. the application of the arbitrary described essence generating and YANG strengthening prescription medicine of claim 1-3 in preparation treatment impotence medicine.