CN105532646A - 肝细胞保存液及其制备方法和应用 - Google Patents
肝细胞保存液及其制备方法和应用 Download PDFInfo
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- CN105532646A CN105532646A CN201610054463.5A CN201610054463A CN105532646A CN 105532646 A CN105532646 A CN 105532646A CN 201610054463 A CN201610054463 A CN 201610054463A CN 105532646 A CN105532646 A CN 105532646A
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- Prior art keywords
- cell
- liver cell
- conserving liquid
- amino acid
- mixed liquor
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- 239000012530 fluid Substances 0.000 title abstract 4
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- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 36
- 239000011259 mixed solution Substances 0.000 claims abstract description 20
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- 239000008354 sodium chloride injection Substances 0.000 claims abstract description 20
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 19
- PXEDJBXQKAGXNJ-QTNFYWBSSA-L disodium L-glutamate Chemical compound [Na+].[Na+].[O-]C(=O)[C@@H](N)CCC([O-])=O PXEDJBXQKAGXNJ-QTNFYWBSSA-L 0.000 claims abstract description 19
- 239000008103 glucose Substances 0.000 claims abstract description 19
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- UYUXSRADSPPKRZ-SKNVOMKLSA-N D-glucurono-6,3-lactone Chemical compound O=C[C@H](O)[C@H]1OC(=O)[C@@H](O)[C@H]1O UYUXSRADSPPKRZ-SKNVOMKLSA-N 0.000 claims abstract description 16
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- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 claims description 16
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 claims description 16
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 16
- 239000004472 Lysine Substances 0.000 claims description 16
- DWAQJAXMDSEUJJ-UHFFFAOYSA-M Sodium bisulfite Chemical compound [Na+].OS([O-])=O DWAQJAXMDSEUJJ-UHFFFAOYSA-M 0.000 claims description 16
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- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 claims description 15
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- 239000008223 sterile water Substances 0.000 claims description 13
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- 238000000034 method Methods 0.000 claims description 12
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- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 claims description 3
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims description 3
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- QDGAVODICPCDMU-UHFFFAOYSA-N 2-amino-3-[3-[bis(2-chloroethyl)amino]phenyl]propanoic acid Chemical compound OC(=O)C(N)CC1=CC=CC(N(CCCl)CCCl)=C1 QDGAVODICPCDMU-UHFFFAOYSA-N 0.000 description 9
- 230000006870 function Effects 0.000 description 9
- IFGCUJZIWBUILZ-UHFFFAOYSA-N sodium 2-[[2-[[hydroxy-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyphosphoryl]amino]-4-methylpentanoyl]amino]-3-(1H-indol-3-yl)propanoic acid Chemical compound [Na+].C=1NC2=CC=CC=C2C=1CC(C(O)=O)NC(=O)C(CC(C)C)NP(O)(=O)OC1OC(C)C(O)C(O)C1O IFGCUJZIWBUILZ-UHFFFAOYSA-N 0.000 description 9
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0226—Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Physiology (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Biophysics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (7)
Priority Applications (1)
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CN201610054463.5A CN105532646B (zh) | 2016-01-27 | 2016-01-27 | 肝细胞保存液及其制备方法和应用 |
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CN201610054463.5A CN105532646B (zh) | 2016-01-27 | 2016-01-27 | 肝细胞保存液及其制备方法和应用 |
Publications (2)
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CN105532646A true CN105532646A (zh) | 2016-05-04 |
CN105532646B CN105532646B (zh) | 2017-10-13 |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109856089A (zh) * | 2018-11-09 | 2019-06-07 | 合肥诺森医学检验有限公司 | 一种细胞保存液的质量检测方法 |
CN111972399A (zh) * | 2020-08-06 | 2020-11-24 | 温州医科大学 | 维持肝细胞活性的保存液 |
CN113383768A (zh) * | 2021-05-22 | 2021-09-14 | 武汉仝干医疗科技股份有限公司 | 一种肝细胞冻存液及其制备方法和应用 |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2000030442A1 (en) * | 1998-11-25 | 2000-06-02 | Abbott Laboratories | Organ preservation solution containing pyruvate |
CN101491238A (zh) * | 2009-03-06 | 2009-07-29 | 李立 | 一种器官保存液 |
CN101919381A (zh) * | 2010-09-06 | 2010-12-22 | 南方医科大学珠江医院 | 一种生物人工肝用肝细胞的保存液及其制备方法 |
WO2013047666A1 (ja) * | 2011-09-29 | 2013-04-04 | 石原産業株式会社 | 生物材料の低温保存用の保存剤及び低温での生物材料の保存方法 |
CN103952370A (zh) * | 2014-04-29 | 2014-07-30 | 周平 | 生物人工肝用细胞源的培养液 |
CN104996396A (zh) * | 2015-07-27 | 2015-10-28 | 中山大学附属第一医院 | 一种离体肝脏灌注保存液 |
-
2016
- 2016-01-27 CN CN201610054463.5A patent/CN105532646B/zh active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
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WO2000030442A1 (en) * | 1998-11-25 | 2000-06-02 | Abbott Laboratories | Organ preservation solution containing pyruvate |
CN101491238A (zh) * | 2009-03-06 | 2009-07-29 | 李立 | 一种器官保存液 |
CN101919381A (zh) * | 2010-09-06 | 2010-12-22 | 南方医科大学珠江医院 | 一种生物人工肝用肝细胞的保存液及其制备方法 |
WO2013047666A1 (ja) * | 2011-09-29 | 2013-04-04 | 石原産業株式会社 | 生物材料の低温保存用の保存剤及び低温での生物材料の保存方法 |
CN103952370A (zh) * | 2014-04-29 | 2014-07-30 | 周平 | 生物人工肝用细胞源的培养液 |
CN104996396A (zh) * | 2015-07-27 | 2015-10-28 | 中山大学附属第一医院 | 一种离体肝脏灌注保存液 |
Non-Patent Citations (2)
Title |
---|
刘鸿凌 等: "原代乳猪肝细胞低温保存的初步探索", 《肝脏》 * |
苏志良 等: "供移植肝脏保存液及肝细胞保护剂研究进展", 《临床肝胆病杂志》 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109856089A (zh) * | 2018-11-09 | 2019-06-07 | 合肥诺森医学检验有限公司 | 一种细胞保存液的质量检测方法 |
CN111972399A (zh) * | 2020-08-06 | 2020-11-24 | 温州医科大学 | 维持肝细胞活性的保存液 |
CN113383768A (zh) * | 2021-05-22 | 2021-09-14 | 武汉仝干医疗科技股份有限公司 | 一种肝细胞冻存液及其制备方法和应用 |
Also Published As
Publication number | Publication date |
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CN105532646B (zh) | 2017-10-13 |
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Address after: 430206 East Lake high tech Development Zone, Hubei Province, high and new avenue, No. 818, No. Applicant after: WUHAN TOGO MEDITECH Co.,Ltd. Address before: 430206 East Lake high tech Development Zone, Hubei Province, high and new avenue, No. 818, No. Applicant before: WUHAN TOGO BIOTECHNOLOGY CO.,LTD. |
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Denomination of invention: Hepatocyte preservation solution and its preparation method and Application Effective date of registration: 20220629 Granted publication date: 20171013 Pledgee: Pudong Shanghai Development Bank Limited by Share Ltd. Wuhan branch Pledgor: WUHAN TOGO MEDITECH Co.,Ltd. Registration number: Y2022420000204 |
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Address after: 4th floor, podium building, Gongtou Building, No. 433-505 Chunhui Road, Lingxi Town, Cangnan County, Wenzhou City, Zhejiang Province, 325000 Patentee after: Zhejiang Tonggan Medical Technology Co.,Ltd. Country or region after: China Address before: No. 818, Gaoxin Avenue, Donghu high tech Development Zone, Wuhan, Hubei 430206 Patentee before: WUHAN TOGO MEDITECH Co.,Ltd. Country or region before: China |
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